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Scylla serrata (often called mud crab or mangrove crab, although both terms are highly ambiguous, as well as black crab) is an ecologically important species of crab found in the estuaries and mangroves of Africa, Australasia and Asia. In their most common form, the shell colour varies from a deep, mottled green to very dark brown.
Method for extracting DNA of eriocheir sinensis without damage and application thereof
PendingCN122357689ACharalesScylla paramamosain
This invention belongs to the field of biotechnology, specifically relating to a non-harmful DNA extraction method for *Scylla serrata* (scuttica crab) and its application. This invention successfully achieves non-harmful DNA extraction from *Scylla serrata* by collecting its swimming leg setae, significantly reducing the impact on the crab's activity and ensuring its survival after sampling. The extraction method of this invention is non-lethal to *Scylla serrata* and possesses good repeatability and high accuracy. Extracting DNA from *Scylla serrata* using its swimming leg setae ensures that the crab's activity remains unchanged, allowing it to survive and perform its life activities normally. Obtaining the DNA sequence of *Scylla serrata* and performing gene markers provides a foundation for genomic selection breeding, facilitating parent selection and offspring breeding in mud crab genetics.
Owner:SHANTOU UNIV