80 results about "Rhizophora apiculata" patented technology

The invention discloses a mangrove plant kandelia candel extraction method based on Sentinel-1 / 2 time sequence remote sensing images, and relates to satellite remote sensing image processing. The method comprises the following steps: generating year-by-year quantile data of an original spectral band and a spectral index by utilizing a Sentinel-1 / 2 time sequence image, and generating 10m elevation, gradient and slope direction data by adopting 30m global elevation data; establishing a research area classification system, and generating a training sample set according to a high-resolution image provided by Google Earth software; and generating a training model based on a random forest classification algorithm to obtain a mangrove plant kandelia candel distribution result. The mangrove plant kandelia candel extraction method based on percentile is designed by using Sentinel-1 / 2 time sequence remote sensing data, and high-precision extraction of the mangrove plant kandelia candel with plaque fragmentation and large scale is realized. The result shows that the spatial distribution condition of the kandelia candel community provides data support for mangrove forest ecological restoration, blue carbon evaluation, ecological system service evaluation and the like.

The invention relates to the technical field of ecological breeding of scylla paramamosain and protection and restoration of mangrove forests. The invention provides a method for culturing scylla paramamosain by using mangrove forest, which comprises the following steps: (1) carrying out simple engineering transformation on the basis of a coastal wetland traditional culture pond, constructing a planting island in the middle of the culture pond, and reserving an annular tidal creek between the planting island and the periphery of the culture pond; (2) planting mangrove plants in the planting island to form a man-made forest; (3) small aquatic plants are planted at the bottom of the artificial forest, and large algae are planted in the annular ditches; and (4) putting the young blue crabs into the culture pond, and carrying out daily management until the blue crabs are harvested. The shellfish is used for providing main food supply for the blue crabs, and meanwhile water is purified; excrement of the blue crabs can be fed back to promote growth of mangroves, algae, mussels and the like; large algae can perform photosynthesis at the bottom of the pond to increase dissolved oxygen in water, and small sea grass is placed in the planting island to make up for the defect of low coverage of newly planted mangrove forest plant seedlings.

The utility model relates to the technical field of mangrove planting, and discloses a mangrove plant planting device which comprises a baffle used for avoiding soil loss, a positioning seat used for locking the baffle and a connecting assembly used for adjusting the position of the positioning seat. The connecting assembly comprises a center base used for loading and supporting the contained structure, a driving gear rotationally arranged in the center base, a gear shaft rod fixedly installed on the driving gear, and a first rotating rod rotationally arranged on the gear shaft rod, wherein a connecting rod and a driven rack are arranged on the center base in a sliding mode. The end, away from the center base, of the connecting rod and the end, away from the center base, of the driven rack are fixedly connected with the positioning base. According to the mangrove plant planting device, the positioning seats are arranged to limit the positions of the baffles, the multiple baffles form a frame structure, the frame structure is inserted into the mud flat, protection is provided, soil around the saplings is prevented from being lost, the cultivation effect of the saplings is guaranteed, and then the survival rate of planting of the mangrove saplings is increased.

The invention discloses a seedling raising and transplanting method for mangrove plant Aegiceras corniculatum based on external application of gibberellin, and belongs to the technical field of saline-alkali plant cultivation and mud flat ecological restoration. The method mainly comprises the four stages of seed disinfection and gibberellin soaking, germination cultivation, seedling cultivation, salinity domestication and mud flat transplanting. After being disinfected, the seeds are soaked in a gibberellin solution with the concentration being 300 mg / L, gibberellin with the same concentration is sprayed regularly during germination, and the germination rate and the germination speed are increased. In the seedling stage, humus soil and mud flat bottom mud mixed matrix is adopted for cultivation, and growth is promoted by spraying 100 mg / L gibberellin to leaf surfaces and injecting 25 mg / L gibberellin to rhizosphere. After seedlings are formed, the seedlings are subjected to step-by-step salinity domestication (5 per thousand to 30 per thousand), gibberellin spraying and rhizosphere injection are carried out at regular intervals, and the stress resistance of the seedlings is enhanced. The method can effectively shorten the seedling raising period of Aegiceras corniculatum, improve the seedling raising efficiency, enhance the adaptive capacity of seedlings to the tidal zone environment, and facilitate rapid recovery of a mangrove forest ecosystem.

This invention discloses a method for hardwood cutting propagation of the mangrove plant *Avicennia marina*, comprising: S1: Selection and preparation of cuttings: In early July, select vigorous, healthy, and disease-free superior single plants, collect robust perennial branches, keep them moist after collection, bring them back to the cutting site, and cut them into cuttings on the same day; S2: Pretreatment of cuttings: Disinfect the cuttings obtained in step (1) by soaking them in a 700-fold carbendazim solution for 10 minutes, and then let them air dry naturally; subsequently, quickly dip the cuttings in a 1000 mg / L NAA and IBA hormone solution for 30 seconds, and insert them into the substrate of the seedbed to a depth of 3-4 cm. This method for hardwood cutting propagation of the mangrove plant *Avicennia marina* provides technical support for the industrialized and large-scale production of superior *Avicennia marina* seedlings, and can also ensure the demand for superior seedlings for the ecological restoration of coastal mangroves.

The invention discloses a preparation method and an instantaneous transformation method of a mangrove plant bamboo joint tree protoplast, and relates to the technical field of plant protoplast preparation and transformation. According to the method, seedling leaves of the mangrove plant bamboo joint tree protoplast serve as experimental materials, the protoplast is obtained through an enzyme lysis method, and then exogenous genes are transferred into the mangrove plant bamboo joint tree protoplast through a PEG-mediated transformation method for transient expression. The method comprises the following steps: firstly, selecting a bamboo joint tree with fewer mangrove family secondary metabolites as a material, and inducing through spectra with different illumination intensities and wavelengths, so that accumulation of leaf secondary metabolites is reduced while the mesophyll cell structure is adjusted, and further, the purposes of promoting cell wall lysis and protoplast separation are achieved. The invention develops a composite enzymolysis formula adaptive to cell wall components of the mangrove tree, and establishes a set of system for maintaining activity and culturing protoplast of the mangrove tree, so that an exogenous gene can be successfully and transiently expressed in the system.

The utility model discloses a surrounding net protection device for an external mangrove cleaning area. The surrounding net protection device comprises a wave dissipation plate, a guide rod, a sliding rod, a connecting transverse rod, a blocking net, a blocking piece, a longitudinal mounting rod, a cage body and a floating body. The blocking net adopts a lagging type floating design, so that floating garbage can be blocked outside the blocking net all the time and is not influenced by rising and falling tide; after flood tide, the lower portion of the blocking net provides a passage for organisms, after ebb tide, the device does not affect passage of benthonic animals, and ecological restoration of a cleaning area is facilitated.

The present invention disclosure relates to a rapid and cost-effective method for the isolation of genomic material from muscle tissues of mangrove crab for the molecular detection of bacterial pathogens. The methods increase the total recovery of genomic DNA from muscle tissues by heating to 100oC in addition to mechanical disruption of tissues by homogenization. The extracted and isolated genomic DNA using the present invention can 10 be used for the molecular detection of bacterial pathogens from muscle tissues of mangrove crab.

The invention discloses a seed preservation method of cryptophytic mangrove plant avicennia marina. The method comprises the following steps: (1) selecting healthy and full avicennia marina seeds without plant diseases and insect pests, removing outer skin, soaking and disinfecting in a carbendazim aqueous solution for 15 minutes, washing with clear water, and airing; (2) soaking the avicennia marina seeds treated in the step (1) in a chitosan solution for 1 min, taking out, and airing; and (3) embedding the avicennia marina seeds treated in the step (2) into a preservation matrix by taking wet sand as the preservation matrix, then putting the seeds and the preservation matrix into a plastic package bag for sealing, so that the seeds are still kept in a buried state in the bag, and finally storing in a dark environment at 14 DEG C. The germination rate of the avicennia marina seeds stored by the method can still reach 40% after 4 months, the storage time of the avicennia marina seeds is remarkably prolonged, and convenience is provided for seedling raising and ecological restoration of the avicennia marina.

The invention relates to the field of plant ecological risk assessment, and discloses a foreign mangrove species ecological influence assessment and risk prediction method, which comprises the following steps: performing biological characteristic information investigation and analysis on target foreign mangrove species to construct a multi-dimensional assessment index system, and performing risk judgment on the target foreign mangrove species according to different index score values, given by the system, of the target foreign mangrove species. And evaluating a risk evolution process after the risk judgment, and outputting a management scheme for the risk. According to the method, the comprehensive influence of external mangrove species can be comprehensively and comprehensively evaluated, key risk points are not missed, meanwhile, the management efficiency is improved, and the decision randomness and uncertainty are reduced.

The invention discloses a mangrove plant community net primary productivity monitoring method based on trunk flow, and relates to ecology and botany. General regulations of mangrove plant community net primary productivity monitoring based on trunk stem flow, mangrove plant stem flow monitoring content and method, plant leaf and crown atmospheric stability isotope monitoring, mangrove plant community net primary productivity calculation method and other basic contents and requirements are given. The method comprises the following steps: monitoring the stem flow density of a sample tree by adopting a plant stem liquid flow monitoring system, obtaining the composition condition of plant leaf juice and 13C in the canopy atmosphere by adopting an isotope analyzer, and obtaining the canopy leaf area index by adopting a fisheye lens and related software; and calculating the real-time net primary productivity of the mangrove plant community according to the monitored data. The method can be applied to monitoring of net primary productivity of various mangrove forests or land forests. The method is accurate in monitoring, high in popularization degree and good in real-time performance, and solves the problems of poor continuity, high operation difficulty and the like in current mangrove plant community net primary productivity measurement and calculation.

This invention provides a method for controlling Spartina alterniflora and promoting natural mangrove restoration based on rolling mulching and in-situ mud slurry, belonging to the field of ecological control technology. The method involves mechanically rolling Spartina alterniflora to flatten most of it and press it into the tidal flat soil. Then, a biodegradable mulch film is laid on top, and mud slurry is extracted and applied to form a mud slurry cover layer. A mangrove seed interception and planting device is then installed on the mud slurry cover layer to prevent mangrove seeds from growing naturally, thereby reducing the cost of Spartina alterniflora control and improving the efficiency of Spartina alterniflora control.

The invention relates to the technical field of internal circulation low-carbon systems, in particular to a mangrove plant acanthus ilicifolius and bostrichthys sinensis pond planting and breeding coupled internal circulation low-carbon system which comprises three biological components, namely mangrove plant acanthus ilicifolius, bostrichthys sinensis and seawater tilapia, and the three biological components coexist in a low-salt seawater environment with the salinity of 5-8 to form a substance circulation chain; according to the method, the tilapia mossambica culture pond serves as a hub, residual feed and excrement of the tilapia mossambica culture pond are conveyed to the acanthus ilicifolius planting area along with circulating water to provide fertility, chippings of mangrove plant acanthus ilicifolius overflow back to the tilapia mossambica culture pond, and therefore the medicinal and edible value of tilapia mossambica is increased, 70-80% of tilapia mossambica meat mixed feed is fed to Nitrogen and phosphorus-rich bottom mud in a tilapia pond cleaned in winter is recycled as acanthus ilicifolius organic fertilizer, so that resource circulation of'feeding trees with fishes and fattening the fishes with the trees' is realized, and input of exogenous pollution is reduced.

The invention relates to a degradable three-dimensional habitat protection body and a mangrove propagule planting method based on the three-dimensional habitat protection body, the three-dimensional habitat protection body comprises a plurality of three-dimensional structure units, each three-dimensional structure unit comprises a top wall and a bottom wall, the whole top wall is of a hollow structure arched towards the direction away from the bottom wall, and the bottom wall is of a hollow structure arched towards the direction away from the bottom wall. The whole bottom wall is of a hollow structure which is arched in the direction away from the top wall, and the bottom wall and the top wall jointly define a hollow cavity. In the tide scouring and wave impact process, the protection body can weaken the instantaneous dynamic pressure peak value perpendicular to the hollow structure, and the pull-out force and shear stress acting on seedlings or attached organisms in the cavity are reduced; meanwhile, the flow velocity and internal shear force in the cavity can be reduced, local scouring and sediment erosion are effectively inhibited, a relatively stable matrix environment is formed, and continuous support is provided for propagule germination and root growth.

This invention relates to a phenolic compound from mangrove-derived fungi and its preparation method, wherein the phenolic compound has the structure shown in compound 1:

The invention relates to a mangrove forest bionic root system synergistic remediation device, and relates to the technical field of ecological remediation and environmental protection, the mangrove forest bionic root system synergistic remediation device comprises a bionic artificial root system enhancing assembly, the bionic artificial root system enhancing assembly comprises an annular liquid preparation pipe and a hollow tubular network, and the upper surface of the annular liquid preparation pipe is fixedly connected with a vertical rod; a bearing frame is installed on the vertical rod, and a supply assembly is arranged on the bearing frame. Through cooperation of the bionic artificial root system enhancing assembly and the supplying assembly, the synchronous effect of directional development of the root system of the mangrove and efficient regulation and control of pollutants is achieved, the bionic artificial root system enhancing assembly serves as the core, the micro holes in the hollow tubular network release the remediation liquid conveyed by the liquid storage box to soil around the root system, growth nutrients are provided for seedlings, and the remediation effect is achieved. Soil heavy metal ions are fixed through components of the remediation liquid, meanwhile, a supporting frame for directional growth is provided, a root system is guided to extend towards the deep layer of the soil, and the problem that root system development is limited in the high-salt and high-temperature environment is solved.

The invention relates to the field of utilization of marine medicinal biological resources, in particular to a chromone derivative as well as a preparation method and application thereof.The chromone derivative Sonnerachromone A-G (1-7) is obtained by extracting and separating mangrove songarus apetala fruits, the chromone derivative Sonnerachromone A-G belongs to a natural active ingredient, the derivative is a new compound, and the chromone derivative Sonnerachromone A-G is a natural active ingredient and is a natural active ingredient. It is found that the chromone derivatives Sonnerachromone A-G have good activity of delaying senescence of nematodes, the senescence delaying activity of part of compounds is superior to that of a positive control group, and the chromone derivatives Sonnerachromone A-G can be used as medicinal or non-medicinal senescence delaying products independently or in a combined mode and have good medicinal and dietary supplement prospects.

The invention discloses an ecological cultivation seedling raising system and method for mangrove planting, and particularly relates to the technical field of seedling raising, and the system comprises a mangrove planting management system, a mangrove water cultivation seedling raising device, an environment adjusting device and a salinity adjusting device. According to the mangrove planting management system, mangrove seedling raising pictures are obtained through the image taking unit, the types and growth states are recognized through the planting analysis module, the planting control module generates irrigation and environment adjusting instructions accordingly, the mangrove hydroponic seedling raising device achieves spraying or tidal irrigation according to the instructions, and the environment adjusting device can adjust environment factors such as temperature, humidity and illumination. The salinity adjusting device can keep the suitable salinity of the water body, and the seedling raising method is based on the system and comprises the steps of obtaining mangrove seedling raising information, determining the maturity grade, generating an instruction, executing irrigation and environment adjustment, monitoring and adjusting the salinity at the same time, and recycling irrigation liquid. Accurate management and efficient cultivation of mangrove seedling raising are achieved, and protection and restoration of a mangrove forest ecological system are facilitated.

The invention provides a floatable mangrove plant support which comprises at least one standard unit, each standard unit comprises a first bamboo raft, a second bamboo raft, a connecting assembly and a bearing assembly, and the first bamboo raft is provided with a plurality of first penetrating holes at intervals; the second bamboo rafts are connected with the first bamboo rafts through fixing bamboo tubes and provided with a plurality of second penetrating holes corresponding to the first penetrating holes at intervals. The connecting assembly comprises a plurality of connecting rod pieces arranged at intervals, each connecting rod piece comprises a tension spring pipe, a tension spring and a transmission pull rod, the tension spring pipes are hinged to the second bamboo rafts, the transmission pull rods are connected with the tension spring pipes through the tension springs, and limiting stop levers are arranged on the transmission pull rods; the bearing assembly comprises a plurality of bearing rod pieces arranged at intervals, the two opposite ends of each bearing rod piece slidably penetrate through the corresponding first penetrating hole and the corresponding second penetrating hole respectively, the bearing rod pieces can be stressed to rotate, a plurality of clamping grooves are formed in the bearing rod pieces in the length direction at intervals, and the clamping grooves can be clamped with the limiting stop rods. The problem that mangrove plants are difficult to transplant in an intertidal zone can be solved.

The application discloses a kind of difficult stand beach intertidal alien mangrove plant Laganum community transformation method, belong to mangrove planting technical field;Steps include: S1, mangrove present situation investigation;S2, beach protection repair measure;S3, Laganum pruning or felling;S4, plant mangrove forest;S5, management and protection;S6, effect evaluation;The method can be grown to Laganum community renewal reconstruction in seawater scouring area, avoid reconstruction failure risk, compared with other conventional methods reconstruction repair early canopy forestation;After taking beach protection measure, the beach of repair area is stable, avoid being lost by tidal scouring and leading to sediment;Use old fishing net to protect, avoid that marine litter enters repair area;Use small block to level, keep original Laganum root system, there are few biologicals in woodland in early reconstruction period, such as crab, reduce the interference and damage to new planting seedling, guarantee mangrove seedling survival rate;Using seedling planting that is cultivated under the condition of high salinity environment, seedling resistance is strong, recovery growth speed is fast, survival rate is high.

The invention discloses an endogenous promoter AMGT1P11 of mangrove plant avicennia marina and application of the endogenous promoter AMGT1P11, and belongs to the technical field of gene engineering, and the nucleotide sequence of the endogenous promoter AMGT1P11 comprises a sequence as shown in SEQ ID NO: 1, a sequence as shown in SEQ ID NO: 2, a sequence as shown in SEQ ID NO: 3 and a sequence as shown in SEQ ID NO: 4; or a sequence which has at least 70% homology with the sequence as shown in SEQ ID NO: 1 and has the same function as the sequence as shown in SEQ ID NO: 1; or, one or more basic groups are added, substituted, inserted or deleted in the sequence shown in SEQ ID NO: 1, and the sequence has the same function as the sequence shown in SEQ ID NO: 1. The AMGT1P11 contains a reported salt-induced cis-acting element GT1-motif (GGTTAA, a new reference can be provided for research of a mangrove plant salt-tolerant mechanism, meanwhile, the promoter can regulate and control expression of exogenous target genes in dicotyledons and monocotyledons, a brand new tool and selection are provided for plant transgenic engineering, and an important foundation is laid for plant genetic improvement.

The present application relates to the field of remote sensing monitoring, in particular to a mangrove inter-specific classification method and device based on unmanned aerial vehicle remote sensing, adopting a decision tree learning algorithm to update feature threshold values in a feature corresponding relationship of a plurality of mangrove plant categories in an initial multi-modal plant knowledge graph, obtaining an updated multi-modal plant knowledge graph, performing inter-specific classification reasoning according to a plurality of types of feature data in the extracted feature data set of the region to be classified, feature values corresponding to the feature data, and the updated multi-modal plant knowledge graph, obtaining a mangrove inter-specific classification result, which integrates classification knowledge, overcomes the shortcomings of a method that simply relies on data, and improves the accuracy, efficiency and reliability of mangrove inter-specific classification.

The invention discloses a culture pond mangrove forest restoration appropriate elevation determination system based on tide level numerical simulation, and relates to the technical field of marine ecological restoration, and the system specifically comprises an acquisition processing module, a tide level model module, a scene simulation module, a relation analysis module, a screening comparison module and an optimization decision module. The system firstly collects basic data; establishing a three-dimensional hydrodynamic numerical model based on a hydrodynamic principle; simulating water level responses under different working conditions according to the basic data and the gate operation rule; calculating the daily average flooding time of any given elevation point in the culture pond in the simulation period through statistical analysis, and establishing a corresponding relation curve between the elevations of different positions and the daily average flooding time; comparing the flood-tolerant duration data of the target mangrove plant species with the corresponding relation curve as a screening condition; and finally, calculating a theoretical appropriate elevation range, and taking the appropriate elevation range determined under the working condition as a basis for guiding the appropriate elevation design for repairing the mangrove forest in the culture pond.

The invention discloses an indoor seedling raising method for mangrove plant red sea olive. The method comprises three stages of seed pretreatment, seed germination and seedling cultivation. The light environment, the temperature, the humidity, the nutrition supply and the salinity of the substrate are accurately regulated and controlled under the condition of full manual control by adopting the culture substrate with a specific proportion, and the dependence of traditional seedling culture on natural conditions is effectively overcome. According to the method, standardization and controllability of red sea olive seedling raising are achieved, the growth speed of red sea olive seedlings is remarkably increased, and the method is suitable for large-scale and annual seedling raising production and has important application value for mangrove forest wetland restoration and ecological engineering.

The invention belongs to the field of marine natural product chemistry, and particularly relates to a mangrove-derived fungus heterocyclic dipeptide compound and application thereof. According to the invention, a secondary metabolite of mangrove-derived fungus XLN32122 is researched, and a new heterocyclic dipeptide compound 1 containing 1, 2-oxa-dodecanoic ring, three analogues (2-4) and three known polyketone compounds (5-7) are separated from the secondary metabolite. An in-vitro anti-inflammatory activity result shows that the compound 3 has remarkable inhibitory activity on the generation of NO in RAW 264.7 cells induced by LPS, the IC50 value of the compound 3 is 13.13 + / -0.005 mu M and is higher than that of a positive control drug dexamethasone, and the IC50 value of the compound 3 is 136.84 + / -1.330 mu M.

The present invention discloses a method and apparatus for simulating the spatiotemporal changes of mangrove vegetation under the influence of a canal. The method includes: first, dividing the simulated area into multiple spatial units; acquiring remote sensing data and extracting relevant information; constructing a dynamic mangrove growth model and a spatial simulation model; and completing the calibration and verification of the model coupling. Multiple canal construction scenarios and post-construction environmental factors are obtained and input into the model to obtain the spatiotemporal simulation results of mangrove vegetation changes corresponding to each scenario. Finally, a target canal construction scenario is determined. This design provides a scientific decision-making basis for achieving a balance between canal construction and mangrove vegetation protection.

The invention discloses a salt-tolerant flooding-resistant mangrove plant variety breeding method, belongs to the technical field of mangrove plant breeding, and systematically carries out salt-tolerant flooding-resistant excellent mangrove variety breeding work through three links of germplasm resource collection, character evaluation and variety breeding. The method comprises the following steps: firstly, carrying out resource census on mangrove plants in a natural distribution area, comprehensively evaluating the growth and resistance performance of the mangrove plants, and collecting at least 50-60 parts of excellent germplasm for local planting and storage; secondly, carrying out salt-tolerant and flooding-tolerant experiments on the collected germplasm, analyzing phenotypic characters and physiological changes of the germplasm, deeply excavating stress-resistant key genes by applying multiple omics technologies such as transcriptome and metabolome, and verifying the expression quantity of the key genes in combination with a qPCR technology; finally, gene expression data and phenotypic resistance analysis results are integrated, salt-tolerant and flood-tolerant mangrove plant varieties rich in genetic background diversity are screened out, a complete technical route of resource collection, omics analysis and directional breeding is formed, and high-quality germplasm resources are provided for mangrove forest ecological restoration.

The invention discloses a cultivation pond mangrove forest reforestation carbon sink prediction method, relates to the technical field of carbon sink evaluation, and aims to solve the problem that related carbon sinks cannot be accurately predicted in the prior art. The method comprises the following steps: respectively measuring carbon dioxide and methane emissions of the mangrove forest and the coastal culture pond and performing conversion calculation to obtain carbon emission decrement; measuring mangrove forest plant carbon sink and soil carbon burial rate to obtain carbon sink increment; and measuring organic carbon reserves of different soils, and calculating the change of the reserves. And finally, integrating the three aspects of data, performing conversion by recovering the mangrove seedlings for 20 years, and calculating the reforestation carbon sink of the mangrove forest in the culture pond. According to the method, the difference of carbon emission and soil carbon sink neglected in the prior art is comprehensively considered, and an effective way is provided for accurately evaluating the reforestation carbon sink of the mangrove forest in the culture pond through an innovative calculation mode.

The present invention relates to the field of utilization of marine medicinal biological resources, and in particular to a class of chromone derivatives, a preparation method and an application thereof. The present invention obtains chromone derivatives SonnerachromoneA-G (1-7) by extracting and separating the fruit of Sonneratia apetala, a mangrove tree. The chromone derivatives SonnerachromoneA-G are natural active ingredients and are new compounds. It is found that the chromone derivatives SonnerachromoneA-G all have good nematode aging activity, and some compounds have better aging activity than a positive control group. The chromone derivatives can be used alone or in combination as medicinal or non-medicinal anti-aging products, and have good medicinal and dietary supplement prospects.

The present invention discloses a method for analyzing the sources of nitrogen pollution in mangroves based on the stable nitrogen isotope composition, which includes: culturing mangrove plants in the laboratory to ensure that the initial isotope values of each plant are consistent; placing cages in a series of mangroves with nitrogen concentration gradients and planting the mangrove plants into the cages; allowing the mangrove plants to grow in the cages for at least one month to ensure isotope integration of the mangrove plants; collecting mangrove plant samples from the cages multiple times, drying the mangrove plant samples, and grinding them into powders to make aluminum-tin capsules; using a stable isotope ratio mass spectrometer (IRMS) to analyze the δ<supgt;15< / supgt;N isotope ratio of the aluminum-tin capsules; drawing spatial variation diagrams, temporal variation diagrams, and hotspot diagrams based on the δ<supgt;15< / supgt;N isotope ratio; and determining the sources of nitrogen pollution according to the differences in the δ<supgt;15< / supgt;N isotope ratio of the mangrove plants. Through the method of the present invention, accurate judgment of the sources of nitrogen pollution in mangroves can be achieved, and it can also provide directions for environmental governance and environmental protection.