13results about How to "Breed fast" patented technology

This invention discloses PtoNAC028 The gene is used to regulate the ratio of pectin monomers in poplar trees. The coding region of this gene has the nucleotide sequence shown in SEQ ID NO:1. This is achieved through overexpression or silencing of the gene in poplar trees. PtoNAC028 Genes capable of increasing or decreasing the proportion of lignin monomers in poplar provide key genes for the genetic improvement of poplar processing traits. This invention also discloses a method for cultivating poplars with a high lignin monomer ratio, through overexpression of [gene name missing] in poplar trees. PtoNAC028 The gene can significantly increase the proportion of lignin monomers in poplar trees, achieving targeted regulation of monomeric lignin metabolism, which helps to rapidly cultivate new poplar varieties with excellent wood properties and fast growth.

The invention belongs to the technical field of wheat molecular breeding, and particularly relates to an SNP molecular marker related to wheat grain hardness on a wheat chromosome. The SNP molecule is named as qHI.1B-1; the molecular marker contains a nucleotide sequence of which the polymorphism at the 393305782bp of the wheat 1B chromosome is A or G; the wheat with the polymorphic site genotype of GG has lower grain hardness compared with the wheat with the genotype of AA. Based on the particularity, the inventor carries out further BSR analysis on an F2 population constructed based on the two varieties, and the SNP site is obtained. Based on the site, a certain foundation can be laid for early and rapid detection and analysis of the wheat hardness character, the subsequent selection efficiency can be improved, the breeding period can be shortened, and wheat varieties with ideal grain hardness can be rapidly identified and cultivated.

The invention discloses an application of an OsHDA701 gene in regulation and control of drought resistance of rice. According to the application of the OsHDA701 gene in regulating and controlling the drought resistance of the rice, the nucleotide sequence of the OsHDA701 gene is as shown in SEQ ID NO. 1. The rice drought resistance is specifically and negatively regulated by the OsHDA701 gene, and the rice drought resistance can be enhanced by knocking out the OsHDA701 gene or inhibiting the expression of the OsHDA701 gene. According to the application, it is clear that the OsHDA701 gene serves as a negative regulatory factor to control the drought resistance of the rice for the first time, and a new molecular target is provided for genetic improvement of the rice. By inhibiting the function of the negative regulation gene OsHDA701, the inherent stress resistance potential of the rice can be released, so that the drought resistance of the transgenic rice is enhanced, and the survival rate of the transgenic rice under drought stress is obviously higher than that of wild rice. According to the method disclosed by the invention, an OsHDA701 gene mutant plant is created through a mature gene editing technology, and the breeding process is accurate and rapid.

The application discloses a kind of based on three-dimensional point cloud image's Pinus massoniana partial phenotypic parameter automatic measurement method. Three-dimensional point cloud image of Pinus massoniana plant under different viewing angles is collected;Pretreatment obtains the three-dimensional point cloud of Pinus massoniana plant;Registration is carried out to obtain fused point cloud;Extract the minimum spanning tree and the skeleton point of main stem, and then obtain the main stem point cloud and leaf point cloud;According to the minimum spanning tree, main stem point cloud and leaf point cloud processing obtain main stem length, Pinus massoniana ground diameter, average leaf length and leaf number.The method of the application can automatically identify Pinus massoniana plant in three-dimensional point cloud image, and complete the strategy of main diameter length, ground diameter, average leaf length, leaf length, improve the detection efficiency, realize high-precision, high-efficiency crop phenotypic data acquisition, achieve fast, low-cost crop phenotypic parameter accurate and nondestructive testing.

This invention discloses a method for promoting somatic embryogenesis and maturation in Cryptomeria japonica, belonging to the field of somatic embryogenesis technology. The method involves using immature seed embryos as explants to induce embryogenic callus; transferring the proliferated embryogenic callus to a somatic embryogenesis induction medium for further culture to obtain somatic embryos; transferring the somatic embryos to a somatic embryogenesis maturation medium for dark culture to obtain mature cotyledonary embryos; transferring the mature cotyledonary embryos to a somatic embryogenesis basal medium for light culture to obtain somatic embryonic seedlings; and hardening off the seedlings before transplanting to obtain regenerated plants. The results of this application show that embryogenic callus can be stably obtained from different families, with high somatic embryogenesis maturation capacity and rate, enabling the cultivation of a large number of high-quality seedlings with stable genetic traits in a short time; avoiding the phenotypic segregation problem caused by gene recombination during seed propagation, and ensuring that offspring plants inherit the superior characteristics of the parents.

The utility model relates to composite culture jar technical field especially relates to bacillus cereus composite culture jar, including support platform and transparent incubation jar, the upper end of support platform is equipped with the transparent incubation jar for cultivating bacillus cereus and being convenient for observation.

The invention discloses a tissue culture and rapid propagation method for arrowroot. The tissue culture and rapid propagation method comprises the following steps: 1) obtaining and disinfecting an explant; 2) adventitious bud induction; (3) carrying out subculture multiplication; 4) rooting and seedling strengthening; and 5) transplanting test-tube plantlets. According to the method, explant adventitious bud induction, multiplication culture and rooting culture are carried out by selecting a new bud of arrowroot as an explant, optimizing a disinfection step and selecting an improved MS culture medium and an improved 1 / 2 MS culture medium to replace a common MS culture medium; the disinfection survival rate of arrowroot, the induction rate of adventitious buds, the multiplication coefficient of cluster buds, the rooting rate of seedlings and the transplanting survival rate are remarkably improved, and a large number of test-tube plantlets can be bred in a short time to meet the market requirements. The technology is simple, affordable, feasible and high in application value. The method can be implemented only by simple plant tissue culture equipment.

The application provides a method for improving the resistance of rice plants to rice black-streaked dwarf virus by using gene editing technology. By using the method, a rice variety with high resistance to rice black-streaked dwarf virus can be quickly bred, and the loss caused by the disease can be reduced.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

The invention belongs to the technical field of microbial remediation, and particularly relates to brevibacillus laterosporus M2H06 and application of the brevibacillus laterosporus M2H06 in degradation of zearalenone. The invention provides brevibacillus laterosporus for degrading zearalenone, the Latin name of the brevibacillus laterosporus is Brevibacillus laterosporus, the brevibacillus laterosporus is preserved in Guangdong Microbial Culture Collection Center, the preservation date is October 27, 2025, the preservation address is Guangdong, China, and the preservation number is GDMCC.No 67167. The brevibacillus laterosporus can be used for degrading zearalenone. And brevibacillus laterosporus M2H06. The brevibacillus laterosporus M2H06 provided by the invention can achieve a degradation rate of 99.25% for 5 [mu] g / mL zearalenone within 12 h, and is efficient, rapid and free of extra pollution. After the zearalenone is removed from the feed, the zearalenone does not need to be removed again.

The application belongs to the field of biotechnology and genetic engineering, and discloses a culture method of double-fluorescently labeled Caenorhabditis elegans for monitoring mitochondrial homeostasis, which comprises the following steps: culturing Caenorhabditis elegans ATU3301 strain to L4 larva stage, and selecting male as the father; culturing Caenorhabditis elegans MQD753 strain to L4 larva stage, and selecting hermaphrodite as the mother; placing them on a culture plate in a certain proportion, mating, and obtaining offspring; removing the parents and continuing to culture the offspring to L2 larva stage; screening Caenorhabditis elegans through a microscope to obtain target Caenorhabditis elegans; without an external staining agent, the fluorescent protein is stably integrated into the genome and specifically located in mitochondria, the signal is stable, the location is accurate, and the repeatability is high, which provides a new tool with physiological relevance, operational convenience and cost advantage for the research on mitochondrial homeostasis mechanism and the development of related disease drugs, and has great potential application value.

The application discloses a microbial remediation agent for heavy metal remediation and a preparation method and application thereof, and is prepared by loading pseudomonas aeruginosa on a carrier; the carrier comprises the following components in parts by weight: 8-22 parts of chlorella vulgaris algae liquid, 1-3 parts of silicon fertilizer, and 1-3 parts of nano zero-valent iron; the silicon fertilizer is obtained by calcining a mixture of granite stone powder, marble powder and alkali activator at a mass ratio of 8-12:0-12:0.5-4; and the alkali activator is solid sodium hydroxide or potassium hydroxide. The microbial remediation agent has high activity, fast reproduction, is environment-friendly and pollution-free, and is low in cost; can change the form of heavy metals, converts the heavy metals from a high-toxicity form into a low-toxicity form, reduces the toxicity and migration of the heavy metals, and significantly reduces the pollution caused by the heavy metals.

This invention discloses a highly efficient sgRNA combination for knocking out the MSTN gene in sheep and its application, belonging to the fields of genetic engineering and animal husbandry genetics and breeding. The sgRNA combination includes sg-M2 and sg-M3, wherein the nucleotide sequence of sg-M2 is shown in SEQ ID NO:1, and the nucleotide sequence of sg-M3 is shown in SEQ ID NO:2. Using the sg-M2 and sg-M3 sgRNA combination provided by this invention, offspring are obtained by microinjecting the sgRNA combination and Cas9 mRNA / protein into sheep embryos and then transferring the embryos. Genotyping of the offspring sheep showed that the MSTN gene editing efficiency reached 100%, with a functional domain knockout efficiency of up to 80.0%. This invention can effectively reduce the preparation cost of MSTN gene knockout sheep and provides an effective means for rapidly creating new breeds of double-muscular-rump mutton sheep.