The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum
tissue culture. The application uses
sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high
germination rate, establishes a regeneration
system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and
gametophyte culture of Brachythecium populeum.