40results about How to "Breed fast" patented technology

The invention discloses a tree shrew hypertension model making method. The method comprises the following steps: (1) solvent preparation: dissolving palmitic acid in absolute ethyl alcohol to prepare 24mg / ml storage liquid for later use; and (2) diluting the stock solution in the step (1) with 10% BSA (Bovine Serum Albumin) into a solution with the concentration of 6mg / ml, and injecting the solution into the abdominal cavity of the tree shrew according to the dosage of 20mg / kg for 21d to finish modeling. The model preparation method is simple, and the model forming rate is high.

The invention provides a soil conditioner for acidified soil remediation and a preparation method thereof, and belongs to the technical field of soil conditioners. The invention discloses a soil conditioner for acidified soil remediation. The soil conditioner is prepared from the following raw materials in parts by weight: 25-30 parts of decomposed organic matters, 15-23 parts of calcined alkaline slag powder, 20-30 parts of a biochar-based composite material, 6-13 parts of a microbial agent and 10-20 parts of a bio-based adhesive. According to the soil conditioner for acidified soil remediation, the unbalance state of acidified soil is improved, and the acidification process is relieved.

The invention discloses an indoor seedling raising method for mangrove plant acanthus ilicifolius. The indoor seedling raising method comprises the steps of seed pretreatment, seed germination and seedling indoor cultivation. The method comprises the following specific steps: putting pretreated acanthus ilicifolius seeds into a seedling raising tray filled with sand, and germinating by controlling temperature, salinity and nutrition conditions. Placing the germinated robust seedlings on a cultivation module, and harvesting acanthus ilicifolius seedlings after the seedlings are cultivated to the required height. According to the acanthus ilicifolius seedling cultivation method, indoor vertical cultivation facilities are utilized, specific light intensity, light period, temperature, salinity and the application amount of organic fertilizer, calcium, magnesium and microelements are set, the limitation on conditions such as soil fertility, climate, sunlight and salinity is eliminated, and rapid and high-density cultivation of acanthus ilicifolius seedlings can be achieved.

The invention relates to motherwort cultivation. The leonurus japonicus rooting induction method is characterized by comprising rooting induction: when adventitious buds grow to 1-3 cm, the adventitious buds are cut off from explants and transferred into a rooting culture medium to be cultured, the rooting culture medium takes an MS culture medium as a basic culture medium, and IBA with the concentration of 0.1 mg.L <-1 > is added into the rooting culture medium. The components of the culture medium are selected, and the rooting rate of the selected rooting culture medium is high.

The invention discloses a hydroponic seedling raising method for mangrove plant kandelia candel, and belongs to the technical field of mangrove plant breeding and seedling raising methods and hydroponic plants. The method comprises the four steps of seed pretreatment, seed field planting, seed germination and seedling water culture. By using the hydroponic seedling culture method, the culture environment conditions (illumination, nutrient elements of the hydroponic solution, temperature and salinity) can be manually regulated and controlled, the constraint of soil, climate, land fertility and sunlight can be got rid of, a new seedling culture thought is provided for artificial afforestation of kandelia candel, and the method has important social and scientific significance.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus buchneri strain LB-09 and application thereof. The preservation number of the lactobacillus buchneri strain LB-09 is CGMCC (China General Microbiological Culture Collection Center) No.31193, and the preservation date of the lactobacillus buchneri strain LB-09 is July 8, 2024. The lactobacillus buchneri strain LB-09 is subjected to submerged fermentation culture, fermentation liquor is applied to soil, leaf surfaces or seed surfaces, including soil application, leaf surface spraying, seed soaking or root dipping and the like, the disease resistance and the growth condition of crops are improved, and the lactobacillus buchneri strain LB-09 has the advantages of being good in biological safety, high in decomposition capacity, easy to apply and the like, and has a good application prospect. And green development and modernization of agriculture can be realized.

The invention discloses a seedling breeding method for improving the survival rate of turmeric. Belongs to the technical field of tissue culture. The turmeric plant is obtained by using a plant tissue culture technology through explant sampling, disinfection, in-vitro culture and out-of-bottle transplanting. According to the method, seedlings can be rapidly propagated, the seedlings are robust, root systems are developed, the survival rate is high, the content of effective components is high, and an effective way is provided for meeting the market requirement of turmeric.

This invention discloses PtoNAC028 The gene is used to regulate the ratio of pectin monomers in poplar trees. The coding region of this gene has the nucleotide sequence shown in SEQ ID NO:1. This is achieved through overexpression or silencing of the gene in poplar trees. PtoNAC028 Genes capable of increasing or decreasing the proportion of lignin monomers in poplar provide key genes for the genetic improvement of poplar processing traits. This invention also discloses a method for cultivating poplars with a high lignin monomer ratio, through overexpression of [gene name missing] in poplar trees. PtoNAC028 The gene can significantly increase the proportion of lignin monomers in poplar trees, achieving targeted regulation of monomeric lignin metabolism, which helps to rapidly cultivate new poplar varieties with excellent wood properties and fast growth.

The present invention provides an antifreeze polypeptide derived from bovine collagen, which can reduce the difference in solute concentration inside and outside the cell during the cooling process, thereby inhibiting the formation of ice crystals inside and outside the sperm cell, and further reducing ice crystal damage to cell membranes and organelles during deep cryopreservation and recovery, thereby improving the survival rate of sperm cells after deep cryopreservation and recovery. The frozen sperm has a higher in vitro fertilization cell development rate and fertility rate, and can reproduce rapidly. In addition, the semen freezing diluent provided by the present application does not contain any exogenous protein, which can effectively reduce exogenous protein interference and immunosuppression during in vitro fertilization, and further improve the fertilization rate and fertility rate of frozen semen.

The invention provides an autotrophic denitrification filter material and a preparation method and application thereof, and belongs to the technical field of water treatment.The autotrophic denitrification filter material comprises the following raw materials: a main body material, an adhesive, a foaming agent and a slow-release agent, and the main body material is sulfur powder, thiosulfate and pyrite powder; the preparation method comprises the following steps: mixing the main materials to obtain a component A, mixing the slow-release agent to obtain a component B, mixing the component A and the component B, adding water, uniformly stirring, adding the adhesive and the foaming agent, heating, uniformly stirring, and granulating to obtain the filter material. The invention provides an autotrophic denitrification filter material as well as a preparation method and application thereof, and aims of obtaining a light filter material and quickly starting biofilm formation are fulfilled.

The utility model relates to the technical field of fermentation tanks, and discloses a fermentation tank convenient for production of fishing yeast cultures, which comprises a tank body, a feed pipe, an exhaust pipe and a hollow shaft motor are mounted on the top surface of the tank body, a rotating rod is mounted on an output shaft of the hollow shaft motor, and a rotating joint is mounted at the top end of the rotating rod. The top end of the rotary connector is connected with a first air inlet pipe, one end, away from the rotary connector, of the first air inlet pipe is connected with a multi-filter, and the right side face of the multi-filter is provided with a second air inlet pipe. The air blower is started, the air blower can input outside air into the multiple filters through the second air inlet pipe, the multiple filters can remove particles, bacteria and other pollutants in the air, and then the pure breathing environment of microorganisms is guaranteed; afterwards, air can enter the air conveying pipe through the first air inlet pipe and the rotary connector and can be evenly sprayed into the tank body through the multiple spray heads.

The invention discloses a method for improving the fermentation level of lactobacillus acidophilus based on culture medium improvement and pH regulation and control, and belongs to the technical field of microbial fermentation. A seed solution of lactobacillus acidophilus is inoculated into an improved culture medium without buffer salt, fermentation is performed at the temperature of 37 DEG C under the constant pH range of 4.5-4.6, in the fermentation process, 2mol / L sodium hydroxide is fed into the solution to keep the pH constant, the fermentation level of lactobacillus acidophilus can be remarkably improved, the viable count of lactobacillus acidophilus CCFM137 fermentation liquor can reach 3.37 * 10 < 9 > CFU / mL, and the pH value of the lactobacillus acidophilus CCFM137 fermentation liquor can reach 3.7 * 10 < 9 > CFU / mL. Meanwhile, the raw material utilization rate and the freeze-drying survival rate are improved, and certain guiding significance is achieved for 100L or larger-scale lactobacillus acidophilus fermentation. The lactobacillus acidophilus culture medium has the advantages of simple formula, high nutrient substance utilization rate, high fermentation efficiency, short fermentation time and the like, and is beneficial to industrial production of lactobacillus acidophilus.

The invention belongs to the technical field of wheat molecular breeding, and particularly relates to an SNP molecular marker related to wheat grain hardness on a wheat chromosome. The SNP molecule is named as qHI.1B-1; the molecular marker contains a nucleotide sequence of which the polymorphism at the 393305782bp of the wheat 1B chromosome is A or G; the wheat with the polymorphic site genotype of GG has lower grain hardness compared with the wheat with the genotype of AA. Based on the particularity, the inventor carries out further BSR analysis on an F2 population constructed based on the two varieties, and the SNP site is obtained. Based on the site, a certain foundation can be laid for early and rapid detection and analysis of the wheat hardness character, the subsequent selection efficiency can be improved, the breeding period can be shortened, and wheat varieties with ideal grain hardness can be rapidly identified and cultivated.

The invention belongs to the technical field of composite microbial agents, and particularly relates to a composite microbial agent for liquid dung treatment and a preparation method and application thereof. The compound microbial agent is a mixed microbial agent of streptomyces isothiocyanate (HBU11), aspergillus niger (Aspergillus niger) HBUN19, bacillus subtilis (HBU29) and Hydrogenobacterium bissanensis HBU34, and the compound microbial agent is a mixed microbial agent of bacillus subtilis (HBU19), bacillus subtilis (HBU29), bacillus subtilis (HBU34), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29) and bacillus subtilis (HBU34). The compound microbial agent is efficient, non-toxic and biodegradable, and can effectively degrade organic matters in the liquid dung and remarkably reduce the content of pollutants such as ammonia nitrogen and total phosphorus in the liquid dung through compound use of various efficient strains, so that harmless treatment of the liquid dung is realized.

The invention discloses an application of an OsHDA701 gene in regulation and control of drought resistance of rice. According to the application of the OsHDA701 gene in regulating and controlling the drought resistance of the rice, the nucleotide sequence of the OsHDA701 gene is as shown in SEQ ID NO. 1. The rice drought resistance is specifically and negatively regulated by the OsHDA701 gene, and the rice drought resistance can be enhanced by knocking out the OsHDA701 gene or inhibiting the expression of the OsHDA701 gene. According to the application, it is clear that the OsHDA701 gene serves as a negative regulatory factor to control the drought resistance of the rice for the first time, and a new molecular target is provided for genetic improvement of the rice. By inhibiting the function of the negative regulation gene OsHDA701, the inherent stress resistance potential of the rice can be released, so that the drought resistance of the transgenic rice is enhanced, and the survival rate of the transgenic rice under drought stress is obviously higher than that of wild rice. According to the method disclosed by the invention, an OsHDA701 gene mutant plant is created through a mature gene editing technology, and the breeding process is accurate and rapid.

The application discloses a kind of based on three-dimensional point cloud image's Pinus massoniana partial phenotypic parameter automatic measurement method. Three-dimensional point cloud image of Pinus massoniana plant under different viewing angles is collected;Pretreatment obtains the three-dimensional point cloud of Pinus massoniana plant;Registration is carried out to obtain fused point cloud;Extract the minimum spanning tree and the skeleton point of main stem, and then obtain the main stem point cloud and leaf point cloud;According to the minimum spanning tree, main stem point cloud and leaf point cloud processing obtain main stem length, Pinus massoniana ground diameter, average leaf length and leaf number.The method of the application can automatically identify Pinus massoniana plant in three-dimensional point cloud image, and complete the strategy of main diameter length, ground diameter, average leaf length, leaf length, improve the detection efficiency, realize high-precision, high-efficiency crop phenotypic data acquisition, achieve fast, low-cost crop phenotypic parameter accurate and nondestructive testing.

The invention discloses paracoccum C4-4 and C5-3, the preservation number of the paracoccum C4-4 and C5-3 is CGMCC (China General Microbiological Culture Collection Center) NO: 31971, the paracoccum C5-4 and C5-3 are classified and named as Paracoccus communis, and the preservation number of the paracoccum C4-4 and C5-3 is CGMCC NO: 31972, and the paracoccum C5-3 is classified and named as Paracoccus communis. Researches show that C4-4 and C5-3 both have strong ecological adaptability and good growth promoting ability, and can improve the environment in the soil by combining with organic materials. The synthetic flora constructed by using the paracoccus comprises the C4-4 and the C5-3. Tests prove that the synthetic flora has the advantages of C4-4 and C5-3, and the flora has higher alkali production and growth promotion capabilities; good ecological adaptability is achieved, the pH adaptation range is 4-8, and manganese stress resistance can reach 5000 micromoles per liter; the pH of the culture medium can be obviously improved, and the alkalization effect is obvious. The soil conditioner capable of improving acid soil and promoting plant growth is prepared by taking the synthetic flora as a microbial inoculum and compounding the microbial inoculum with an organic material. When the modifier is applied to acid and lean soil, the soil pH can be remarkably increased, the content of organic matters and nitrogen phosphorus and potassium in the soil is increased, and root growth is promoted.

The invention discloses a preparation process of a royal jelly fermented bee product, which comprises the following steps: fermenting bee food to obtain a bee food product with high nutritional ingredients, enhancing the absorption of worker bees and the yield of royal jelly, and adding substances for increasing the tolerance of the bees into the bee food to improve the metabolism of the bees to pesticide residues, thereby reducing the pesticide residues in the royal jelly and improving the yield of the royal jelly. And finally, the collected royal jelly is subjected to modified protease treatment, so that the nutrition and health-care functions of the royal jelly are improved, the taste is improved, and the obtained bee product has the advantages of high oxidation resistance, immunity enhancement, liver protection and the like.

The invention relates to the technical field of mineral processing, in particular to a desulfurization smelting process of high-sulfur tin concentrate, which comprises the following steps: ore pretreatment: crushing, grinding and screening tin ore to obtain ore powder; culturing bacteria and inoculating, namely inoculating bacteria suitable for a high-sulfur environment into a small-scale culture tank, and culturing in a sterile environment; leaching reaction: mixing the pretreated mineral powder with water, adding a bacterial culture solution to prepare ore pulp, and leaching the ore pulp; and solution treatment and tin recovery: carrying out solid-liquid separation on the ore pulp, recovering tin metal and treating waste liquid. According to the desulfurization smelting process of the high-sulfur tin concentrate, the problems of environmental pollution and high cost existing in the traditional smelting process are effectively solved through a bacterial leaching method, and bacteria can rapidly grow and breed in a high-sulfur environment, so that the desulfurization efficiency is improved, and the production cost is reduced. The process provided by the invention is simple and convenient to operate, low in cost and environment-friendly, and the recovery rate of tin can be remarkably improved.

The present invention belongs to the technical field of microbial degradation, and relates to a strain of Bacillus sabii and its cultivation method and application. The bacterium is Bacillus sabii, which is preserved in the Guangdong Provincial Microbial Culture Collection Center and its taxonomic name is Bacillus safensis subsp .safensis The deposit number is GDMCC No: 64077. The Bacillus sabdariffa DWC-2 of the present invention can grow by using petroleum hydrocarbons as a single carbon source, has a high ability to degrade petroleum, produces biosurfactants, has a high oil washing efficiency, and can be applied to microbial enhanced oil recovery, production of biosurfactants / emulsifiers, and restoration of petroleum-contaminated environments.

The present invention relates to a Yunnan micrococcus and its application in passivating heavy metals in livestock and poultry excrement treatment, belonging to the field of heavy metal pollution remediation. The present invention provides Yunnan micrococcus TW001, and a microbial preparation is obtained after strain activation, liquid seed preparation and liquid fermentation. The Yunnan micrococcus TW001 microbial preparation for poultry excrement passivation treatment provided by the present invention is used in soil heavy metal passivation treatment, has the advantages of fast strain growth rate, high fermentation density, strong genetic stability, good compatibility with the environment, and passivation effect on multiple heavy metals such as arsenic, cadmium and mercury, etc., is a good heavy metal biological passivator, and has good application prospects.

The invention relates to the field of genetic engineering and plant genetic breeding, in particular to application of a wheat TaPND gene. According to the invention, a gene TaPND is taken as a target, an sgRNA sequence based on CRISPR / Cas9 is designed, and a DNA fragment for coding the sgRNA sequence is connected to a vector carrying CRISPR / Cas9, so that a plant binary expression vector capable of knocking out the TaPND gene in wheat is constructed; the TaPND gene knockout progeny is obtained through genetic transformation of wheat, and the wheat mutant with advanced flowering time is obtained. Experiments prove that the flowering time of the wheat is advanced by the transgenic wheat with the TaPND gene knocked out. The technical scheme provided by the invention provides a feasible method for realizing rapid wheat breeding by utilizing a genetic engineering technology, and has important breeding application value and wide market application prospect.

The invention discloses a method for modeling a tree shrew hypertension model, comprising: (1) preparing a solvent: dissolving palmitic acid in anhydrous ethanol to prepare a 24 mg / ml storage solution for standby use; (2) diluting the storage solution in step (1) with 10% BSA to prepare a 6 mg / ml solution, and then administering the solution to the tree shrew by intraperitoneal injection at a dose of 20 mg / kg for 21 days to complete the modeling. The model preparation method is simple and has a high modeling rate.

The invention provides a closely linked SSR marker and application thereof in barley helminthosporium leaf spot resistant QTL positioning, and relates to the technical field of molecular marker assisted breeding. The two QTL sites closely linked with the helminthosporium leaf spot resistance character are successfully positioned through the SSR marker technology, the accuracy of the SSR marker is further verified, the positioning work of the barley helminthosporium leaf spot resistance QTL is successfully completed within a short time by utilizing the SSR marker technology, timely and effective support is provided for disease-resistant breeding, and the method has the advantages of being high in practicability and high in practicability. According to the calculated QTL effect, the position of the QTL on the genetic map is determined, fine positioning of the QTL can be achieved, cross breeding is conducted through the screened helminthosporium leaf spot resistant genotype, a new helminthosporium leaf spot resistant barley variety can be more quickly cultivated in combination with a traditional breeding method, and the strategy of the modern genetic technology and the traditional breeding method is combined.

This invention discloses a method for promoting somatic embryogenesis and maturation in Cryptomeria japonica, belonging to the field of somatic embryogenesis technology. The method involves using immature seed embryos as explants to induce embryogenic callus; transferring the proliferated embryogenic callus to a somatic embryogenesis induction medium for further culture to obtain somatic embryos; transferring the somatic embryos to a somatic embryogenesis maturation medium for dark culture to obtain mature cotyledonary embryos; transferring the mature cotyledonary embryos to a somatic embryogenesis basal medium for light culture to obtain somatic embryonic seedlings; and hardening off the seedlings before transplanting to obtain regenerated plants. The results of this application show that embryogenic callus can be stably obtained from different families, with high somatic embryogenesis maturation capacity and rate, enabling the cultivation of a large number of high-quality seedlings with stable genetic traits in a short time; avoiding the phenotypic segregation problem caused by gene recombination during seed propagation, and ensuring that offspring plants inherit the superior characteristics of the parents.

The invention belongs to a method for preparing an organic fertilizer through continuous and rapid fermentation of furfural residues. The preparation method comprises the following steps: adjusting the pH value of furfural residues, and adding a fermentation detonating agent and a microbial agent into the furfural residues after the pH value is adjusted; uniformly mixing the materials to form a fermented material pile for fermentation; when the temperature of the fermentation material pile is higher than 60 DEG C, adding a new material into the fermentation material pile and mixing with the fermentation material pile to form a master batch; when the temperature of the master batch is higher than 60 DEG C, the sub-materials are moved out of the master batch pile, new materials are added into the master batch pile after the sub-materials are moved out, and the sub-materials are the first batch of sub-materials; when the temperature of the master batch is larger than 60 DEG C again, the sub-materials are moved out of the master batch pile, new materials are added into the master batch pile after the sub-materials are moved out, and the sub-materials are two batches of sub-materials; the fermentation completion time of the first batch of sub-materials is 8-10 days after the fermentation of the fermentation material pile, and the fermentation completion time of the second batch of sub-materials is 8-10 days after the new materials are added for the first time; the device has the advantages that the furfural residues can be continuously fed, and the organic fertilizer is continuously produced.

The utility model relates to composite culture jar technical field especially relates to bacillus cereus composite culture jar, including support platform and transparent incubation jar, the upper end of support platform is equipped with the transparent incubation jar for cultivating bacillus cereus and being convenient for observation.

The invention discloses a tissue culture and rapid propagation method for arrowroot. The tissue culture and rapid propagation method comprises the following steps: 1) obtaining and disinfecting an explant; 2) adventitious bud induction; (3) carrying out subculture multiplication; 4) rooting and seedling strengthening; and 5) transplanting test-tube plantlets. According to the method, explant adventitious bud induction, multiplication culture and rooting culture are carried out by selecting a new bud of arrowroot as an explant, optimizing a disinfection step and selecting an improved MS culture medium and an improved 1 / 2 MS culture medium to replace a common MS culture medium; the disinfection survival rate of arrowroot, the induction rate of adventitious buds, the multiplication coefficient of cluster buds, the rooting rate of seedlings and the transplanting survival rate are remarkably improved, and a large number of test-tube plantlets can be bred in a short time to meet the market requirements. The technology is simple, affordable, feasible and high in application value. The method can be implemented only by simple plant tissue culture equipment.

The application provides a method for improving the resistance of rice plants to rice black-streaked dwarf virus by using gene editing technology. By using the method, a rice variety with high resistance to rice black-streaked dwarf virus can be quickly bred, and the loss caused by the disease can be reduced.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.