368 results about "Brown planthopper" patented technology

The invention provides a brown planthopper resistant rice gene Bph9 as well as a molecular marker and application thereof. The brown planthopper resistant rice gene Bph9 has a nucleotide sequence as shown in SEQ ID No.1 and a cDNA (complementary Desoxvribose Nucleic Acid) sequence as shown in SEQ ID No.2. The Bph9 gene is located between molecular markers InD2 and RM28466. The molecular markers closely linked with the gene also includes one of RM28438, InD28450, InD28453, InD14, InD28432, RM28481 and RM28486 which can be used for sieving the rice containing the brown planthopper resistant gene Bph9. The Bph9 gene belongs to the NBS-LRR (Nucleotide Binding Site-Leucine Rich Repeat) gene family; the encoded protein is related to the plant disease resistance; the Bph9 gene is transferred into common rice by virtue of genetic transformation and hybridization, and thus the resistance of rice to brown planthopper can be improved, so that the damage of brown planthopper is decreased, and the purposes of yield increase and yield stabilization are realized.

The present invention belongs to the molecular genetics field and relates to the molecular marker method of the major gene for brown planthopper resistance in the rice variety Rathu Heenati. Via genetic linkage analysis of the individual F2 plant genotype and the brown planthopper resistance level of each F2:3 family produced by hybridizing the insect-resistant variety Rathu Heenati with insect-susceptible variety 02428, the molecular markers RM8213 and RM5953 of the major gene loci Qbph4(Bph3) in the insect-resistant variety Rathu Heenati are obtained, the loci Qbph4(Bph3) is 3.6cM and 3.2cM distant from the markers RM8213 and RM5953 respectively. Detection of this major gene loci in Rathu Heenati and its derivatives via these molecular markers will increase the selection efficiency of brown plant hopper resistant rices.

The invention provides a brown planthopper resistance gene Bph14 in rice. The gene has a nucleotide sequence shown in SEQ ID No. 1 and a cDNA sequence thereof is shown in SEQ ID No.2. The gene Bph14 of the invention belongs to CC-NBS-LRR gene family, and coded protein is related to plant disease resistance. The gene Bph14 has function of resisting the brown planthopper. Through genetic transformation and hybridization, the gene Bph14 is transferred into normal rice varieties, which can improve the resistance of the rice to the brown planthopper, thus reducing the damage caused by the brown planthopper and achieving the purposes of increasing and stabilizing the production of rice.

The invention relates to a molecule tagging method for a brown paddy plant hopper resistant master gene Bph3 of rice cultivars, which belongs to the technical field of genetic thremmatology. The brown paddy plant hopper resistant master gene Bph3 of an insect resistant variety Rathu Heenati is obtained by genetic linkage analysis of the brown paddy plant hopper resistant level of gene types of various single plants of F2 and various genealogies of F 2:3 obtained after hybridization of the insect resistant rice variety Rathu Heenati (female) and an insect susceptible variety 02428(male). The gene is positioned between a molecular marker A4 and a molecular marker RM16533, and the selection efficiency of three Indel indexes namely RH784, RH786 and RH007 of the interval is approximately 97 percent. Detection is made whether the insect resistant variety Rathu Heenati and derivative varieties (systems) of the insect resistant variety Rathu Heenati contain the master gene through the molecular markeres of the brown paddy plant hopper resistant master gene; the brown paddy plant hopper resistance level of the master gene can be predicted; and the selection efficiency of brown paddy plant hopper resistant rice can be greatly improved.

The invention discloses an ultra-low-volume liquid preparation containing clothianidin, which uses clothianidin, or clothianidin and active component II as the active ingredient, and supplements the rest to 100% with auxiliary agents and solvents. Low-volume liquid preparation; active component II is any one of beta-cypermethrin, beta-cyfluthrin, chlorantraniliprole, flubendiamide, abamectin or emamectin benzoate. The invention can be used to control crop pests such as rice planthopper, rice leaf roller, brown planthopper, white-backed planthopper, striatellus striatellus, stem borer and rice thrips, and has the advantages of convenient processing, water saving, high work efficiency and good drug effect , long-lasting, synergistic and other advantages.

The invention provides a molecular marker of major gene Bph6 resistant to brown planthopper and an application thereof. The invention can obtain a pest-resistant cultivar Swarnalata resistance gene Bph6 and position the resistance gene between molecular marker Y1 and molecular marker Y9 by hybridizing a rice pest-resistant cultivar Swarnalata with 9311 (female) to obtain the genetype of each F2 plant and performing genetic linkage analysis by combining resistance levels resistant to brown planthopper of each family of F2:3. The molecular marker linked with the resistance gene is one of RM16994, Y19, Y9, RM119, RM5757, RM16999, RM17004 and RM17008. The molecular marker in the invention can effectively detect whether the major gene locus exists in the pest-resistant cultivar Swarnalata and derived varieties (strains) thereof, thus greatly improving the selection efficiency of rice resistant to brown planthopper and obtaining brown planthopper resistant rice varieties containing Bph6 gene.

The invention relates to a gene marker of a major gene Bph14 for resisting brown planthopper in rice and application thereof. The gene marker Bph14-M1 of Bph14 has the following sequences of forward and reverse primers: a forward primer of (5'-3') AGCCACTTGGTGAACTTATT, and a reverse primer of (5'-3') GATTGACGATGAGGAGACTT. The application method includes 1) extracting rice genomic DNA; carrying out PCR amplification on the rice genomic DNA by using the forward and reverse primers; and carrying out electrophoresis detection on amplification products. The invention employs the marker to assist selection and can accurately transform the brown planthopper resistant gene Bph14 in B5 to a brown planthopper susceptible cultivar and greatly improve the breeding efficiency of brown planthopper resistant varieties.

The invention relates to a molecular marker of an anti-nilaparvata-lugens major gene Bph3 of rice and belongs to the technical field of genetic breeding. A genetic linkage analysis is preformed on a genotype of each single plant of F2 obtained by crossing an rice anti-insect variety RathuHeenati (female) with an insect-susceptible variety 02428 (male) as well as on an anti-nilaparvata-lugens level of each family of F2: 3, thereby obtaining the anti-nilaparvata-lugens major gene Bph3 of the anti-insect variety RathuHeenati. The gene is positioned between the molecular marker A4 and the molecular marker RM16533; the selection efficiencies of three Indel markers RH784, RH786 and RH007 in the interval are around 97%. The molecular marker of the anti-nilaparvata-lugens major gene is used for detecting whether the major gene is contained in the anti-insect variety RathuHeenati as well as derived varieties thereof (family), thereby forecasting the anti-nilaparvata-lugens level; therefore, the selection efficiency of the anti-nilaparvata-lugens rice is improved greatly.

The invention belongs to the technical field of preparation of a rice molecular marker, and in particular relates to a molecular marker for two rice brown planthopper-resistance major genes QBph3 and QBph4 which are simultaneously derived from a pest-resistance introgression line IR02W101. The molecular marker comprises the following steps: carrying out crossing, self-crossing and backcrossing on Zhenshan97, which is an herbivore-susceptible variety, and IR02W101 so as to obtain genotypes of various BC1F2 single plants; carrying out genetic linkage analysis in accordance with brown planthopper-resistance grades of various F2: 3 lines during seedling stage; precisely locating a resistance gene QBph3 of the IR02W101 between long-arm markers t6 and f3 of the 3rd chromosome so as to obtain a co-segregative marker c3-14 as well as closely linked markers q1 and m3; and precisely locating QBph4 between short-arm markers p17 and xc4-27 of the 4th chromosome so as to obtain closely linked markers p6, p9, c4-5, xc4-7, HJ16, J417 and IN156. The molecular marker disclosed by the invention can be used for effectively detecting whether the pest-resistance introgression line IR02W101 and derived varieties thereof contain the major gene site or not.

The invention discloses a carrier plant system for protecting and increasing the number of rice field rice planthopper parasitic wasps. The carrier plant system comprises three parts, namely, carrier plants, substitute hosts and the parasitic wasps, wherein the carrier plants are grassy weed blighted grain grass and clubhead cutgrass herbs, the substitute hosts are main phytophagous insect false brown planthopper on the grassy weed blighted grain grass and the clubhead cutgrass herbs, and the parasitic wasps are mainly anagrus nilapartvatae pang et wang. The system servers as a species pool of natural enemies of the rice planthopper and used for unceasingly breeding the parasitic wasps, the biological control efficiency of the rice planthopper can be effectively improved, and the number of the times of using pesticides can be lowered by 2 to 3 in one season. The system has the advantages of being long in action time, easy to operate and high in practicability.

The invention relates to an induction-enhanced tissue specific promoter and application thereof, belonging to the technical field of plant genetic engineering. A promoter (BPHP, i.e. Bph14 promoter) for Oryza Sativa L. genes resistant to brown plant hoppers is separated from Oryza Sativa L. and is cloned. The promoter has a nucleotide sequence shown as SEQ ID No. 1, can control Oryza Sativa L. genes resistant to the brown plant hoppers to be specifically expressed in vascular bundle tissues and can be expressed in a way that the feeding induction of the brown plant hoppers is enhanced. The promoter contains BS1EGCCR cis-acting elements and can enable the genes to be specifically expressed in plant vascular bundle tissues. Moreover, the promoter has defense-related response cis-acting elements which can be combined with WRKY transcription factors, and the gene expression is increased when Oryza Sativa L. is fed by the brown plant hoppers or is infected by pathogenic bacteria. The promoter can be used as the promoter for the specific expression in the vascular bundle tissues and can be used as the brown plant hopper and pathogenic bacteria induction-enhanced promoter.

The invention relates to serratia marcescens S-JS1 and application of serratia marcescens S-JS1 in pest control. Serratia marcescens S-JS1 solid culture bacteria or bacterium suspension obtained through fermentation culture can both be applied to prevention and treatment of brown planthopper, beet armyworm and prodenia litura. After bacterium suspension of serratia marcescens S-JS1 is sprayed to brown planthopper, the result shows that the correcting death rates of third-instar nymphs, long-wing adult insects and short-wing adult insects of brown planthopper can reach 67.47%, 63.58% and 78.28% within 5 days respectively through the bacterium suspension with concentration of 8*109 cfu/ml; after 100 micro litters of the strain fermentation liquid with concentration of 108 cfu/ml are added to feed blocks (1*1*1 cm), the correcting death rates of third-instar nymphs of beet armyworm and prodenia litura are 45.64% and 32.20% respectively five days after the nymphs eat the feed blocks; the correcting death rates of third-instar nymphs of beet armyworm and prodenia litura are 76.36% and 62.06% respectively 9 days after the nymphs eat the feed blocks.

The present invention relates to a multi-effect flowable concentrate for seed coating, which comprises the effective ingredients of thiamethoxam and fludioxonil. The multi-effect flowable concentrate for seed coating of the present invention contains 1-55% of thiamethoxam, preferably 5-40%, and 1-65% of fludioxonil, preferably 5-40%. The multi-effect flowable concentrate for seed coating of the present invention can be used for seed coating, has characteristics of long duration, low toxicity and remarkable synergism, and can be applied to control of soil insects such as grub, mole cricket and wireworm in peanut, corn, wheat and rice, and control of insects with sucking mouth part, such as seedling aphide, brown planthopper, thrips, powdery mildew and leafhopper, and control of diseases of fusarium wilt, banded sclerotial blight, full rot, rice blast, powdery mildew and bacterial wilt.

The invention discloses application of a NlInR gene of brown planthopper as a target point in preparation of a pesticide for preventing and treating the brown planthopper, and provides dsRNA used forinhibiting expression of the NlInR gene, wherein the NlInR gene is composed of a NlInR1 gene and a NlInR2 gene; the nucleotide sequence of the NlInR1 gene is shown as SEQ ID NO.1; the nucleotide sequence of the NlInR2 gene is shown as SEQ ID NO.2. The effect of the NlInR gene in preventing and treating the brown planthopper is discovered for the first time, the dsRNA of the NlInR gene is obtainedthrough an RNAi technology, and after the NlInR gene is silenced through a micro-injection mode, the individual development and the ovarian development of the brown planthopper can be effectively inhibited, and the spawning amount is reduced, so that the propagation of the brown planthopper is inhibited, and the prevention and treatment of the brown planthopper are realized.

The invention discloses serratia marcescens NlM280 and an application thereof as an insecticide. A bacterial suspension obtained by the fermentation culturing of the serratia marcescens NlM280 or a supernatant obtained by centrifuging the bacterial suspension can be used for preventing and treating brown planthopper or chilo suppressalis. The results of injecting or feeding the bacterial suspension or the fermented supernatant of the serratia marcescens NlM280 into the brown planthopper show that the fatality rate of the brown planthopper after being injected with the bacterial suspension for 2 days can reach 100%; the fatality rate of the brown planthopper after being fed with the bacterial suspension for 3 days can reach 79.8%; the pathogenic effects of the serratia marcescens NlM280 are not influenced if the supernatant of the fermentation liquor of the serratia marcescens NlM280 is treated at the temperature of below 55 DEG C; the fatality rate of the brown planthopper after being injected with the fermented supernatant for 2 days can reach 97.2%; the effect on the brown planthopper after being fed with the fermented supernatant is hysteretic, i.e., the fatality rate of the brown planthopper after being fed with the fermented supernatant for 6 days reaches 84.2%. After the bacterial suspension or the fermented supernatant is sprayed to rice, if the rice is fed to the chilo suppressalis, the fatality rate of the bacterial suspension or the fermented supernatant to the chilo suppressalis can reach above 80.0% at the 4th day.

The invention provides application of OsEXP10 gene in regulating rice growth and resisting nilaparvata lugens. Specifically, the invention finds an EXP10 gene or protein thereof unexpectedly for the first time. Analysis of the expression mode of the gene finds that increase or decrease of expression quantity or activity of EXP10 gene or protein thereof in plants (such as rice) can remarkably improve agronomic character of plants for the first time.

The invention discloses a brown planthopper gene Nl1860 as well as an encoding product and application thereof. The gene Nl1860 has the DNA sequence shown in SEQ ID No. 1. The DNA sequence is composed of 1454 nucleotide bases and contains a complete encoding frame composed of 1386 nucleotides, and the coded proteins contain a protein containing 461 amino acid residues. Research finds that the gene is related to the feeding, survival and egg laying amount of brown planthopper. An RNAi vector for the Nl1860 is constructed and then is transferred into rice to express dsRNA of the gene Nl1860 in the transgenic rice body, so as to achieve the purpose of improving the resistance to brown planthopper of rice. The brown planthopper gene Nl1860 can be widely applied to crop breeding, particularly breeding of insect-resistant rice.

The invention discloses a brown planthopper and whitebacked planthopper repellent as well as a preparation method and application thereof, and belongs to the technical field of agricultural production. The repellent comprises the following components: cinnamon essential oil, notopterygium incisum essential oil, citronella essential oil, eucalyptus oil essential oil, zanthoxylum essential oil, high lipid film and Tween-80. The repellent provided by the invention has the advantage of high safety, has two functions of protection and insect expelling, and is simple in preparation method and convenient to use; the repellent provided by the invention is prepared from natural plant essential oils, is safe and harmless to rice seedlings, repels feeding of adults and nymphs of brown planthopper and whitebacked planthopper and spawning of female adults so as to effectively reduce damage and population quantity of brown planthopper and whitebacked planthopper, does not repel a predator spider, and has advantages of low cost, high efficiency, environmental friendliness, safety for human and animals and the like; the repellent provided by the invention has a broad industrialization prospect, is particularly suitable for use in pollution-free production of rice, and has broad economic, social and ecological benefits.

The invention discloses a botanical synergistic compound pesticide. The botanical synergistic compound pesticide consists of ginkgo leaf methanol extract as an insecticidal agent, Chinese holly methanol extract as a synergist and an emulsion in water prepared by mixing other auxiliaries. The botanical synergistic compound pesticide is free from any chemical component, low-toxic to human and animals, and environment-friendly; and the botanical synergistic compound pesticide can prevent and control brown planthopper, small brown planthopper, bemisia tabaci, aphid, citrus fruit fly, ampullaria gigas, wiggler and other insects.

The invention provides a molecule marker of brown planthopper resistant gene. According to the present invention, through the hybridization and backcrossing of a resistant parent RBPH54 and a susceptible variety TN1, progenies are obtained. Through respective resistance identifications and molecule genetic linkage analysis of the progeny strains, a brown planthopper resistant major recessive genebph20(t) is obtained. Two nearest molecule markers linked beside bph20(t) are BYL7 and BYL8, which are self-developed SSR markers. The distances from the markers to bph20(t) are 1.3cM and 1.2 cM. With molecule markers linked to the brown planthopper resistant gene, the detection can be carried out to detect whether the pest resistant variety RBPH54 or the derivative varieties (systems) of RBPH54 contain the pest resistant genetic locus. Therefore, the selection of brown planthopper resistant paddy varieties or paddy qualities can be substantially improved in efficiency.

The invention discloses a method for preventing and controlling brown planthopper by using harpactor fuscipes. The method comprises the following steps: step one, sampling the brown planthopper in a target rice field by using a five-point sampling method and identifying the age of the brown planthopper; step two, counting the density of the brown planthopper at the age of 2-3 in the target rice field by using an investigation and sampling method; and step three, prevention and control operation: draining accumulated water of the target rice field, and releasing nymphs at the age of 4 and nymphs at the age of 5 of the harpactor fuscipes at single times, wherein the number ratio of the nymphs at the age of 4 and the nymphs at the age of 5 of the harpactor fuscipes to the brown planthopper at the age of 2-3 is 2: 1.5-2: 60. The user amount of chemical pesticide on rice is reduced, pesticide residue of the rice field is reduced, the ecological environment of a farmland is protected, and reliable technical guarantee is provided for food safety.