262 results about "Brown planthopper" patented technology

According to the brown planthopper lethal gene Nlpfam and the application thereof in biological prevention and control, the brown planthopper lethal gene Nlpfam can cause brown planthopper death after being interfered, so that dsRNA or an interference reagent is prepared through transcription of the brown planthopper lethal gene Nlpfam, the Nlpfam gene can be effectively interfered after the dsRNA or the interference reagent is injected into the brown planthopper, the abdomen of the brown planthopper becomes black, and the brown planthopper death can be inhibited. The brown planthopper can be effectively killed, and the biological control on the brown planthopper is realized.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention belongs to the technical field of genetic engineering, and discloses a bacillus thuringiensis-sourced brown planthopper-resistant insecticidal protein and a coding gene and application thereof, the bacillus thuringiensis-sourced brown planthopper-resistant insecticidal protein is an insecticidal protein orf1715, the amino acid sequence is shown as SEQ ID NO: 1, and the nucleotide coding sequence of the gene is shown as SEQ ID NO: 2. The gene is well expressed in an escherichia coli system in a soluble form, and the purified protein has a remarkable specific insecticidal effect on rice pest brown planthopper, so that the gene can be applied to special prevention and control of brown planthopper, and a candidate gene resource is provided for cultivation of a new transgenic insect-resistant rice variety.

The invention discloses application of brown planthopper NlCP6 protein and a coding gene thereof in regulation and control of brown planthopper resistance of plants, and relates to the technical field of genetic engineering prevention and control of rice brown planthopper. By inhibiting the expression of the coding gene of the NlCP6 protein in the brown planthopper, the growth and development of the brown planthopper can be obviously inhibited; aiming at overexpression of the nilaparvata lugens NlCP6 gene, an overexpression vector of the gene is constructed and transferred into a rice plant, so that the nilaparvata lugens resistance of the plant is greatly improved, and the weight gain and survival rate of the nilaparvata lugens eating the transgenic plant are remarkably reduced. The application method provided by the invention shows that the nilaparvata lugens NlCP6 gene plays a very important role in improving the resistance of rice nilaparvata lugens and inhibiting the growth and development of nilaparvata lugens, and has a wide application prospect; the method has great application value for reducing pesticide use, maintaining ecological balance and realizing sustainable development.

The present invention discloses a method for controlling rice brown planthopper based on sustained-release nano-matrine, belonging to the field of nano-pesticides. In the present invention, matrine is loaded into the mesoporous structure of mesoporous silica, and then encapsulated with chitosan to obtain sustained-release nano-matrine. Then, the sustained-release nano-matrine is formulated into a sustained-release nano-matrine solution, which is then applied as a pesticide to the leaves of rice seedlings to control rice brown planthopper. The sustained-release nano-matrine adopted in the present invention can induce rice seedlings to produce the plant defense hormone jasmonic acid precursor 12-oxophytodienoic acid and the brown planthopper-resistant substances luteolin and cis-aconitine. These substances have a targeted toxic effect and can inhibit the reproduction of offspring, thereby better controlling rice brown planthopper.

The invention discloses application of a gene OsRGA2 to improvement of disease and insect pest resistance of rice, and the gene OsRGA2 can enhance the disease and insect pest resistance of rice to brown planthopper, bacterial leaf blight and the like, and is used for cultivating a new variety of disease and insect pest resistant rice.

The invention provides a closely linked molecular marker primer of a rice brown planthopper resistant major gene Bph56 as well as a marking method and application of the closely linked molecular marker primer. According to the invention, genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety C127 (or) and KW (or) are combined with brown planthopper resistance levels of an F3 family for genetic linkage analysis, a resistance major gene Bph56 carried by the insect-resistant variety C127 is identified, and a region of the resistance major gene Bph56 is reduced to a 77kb fragment limited by markers 6M26.278 and 6M26.355 on two sides and is closely linked with a molecular marker 6M26.355. The molecular marker 6M26.355 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C127 and derived varieties (lines) of the insect-resistant variety C127 contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper-resistant character is greatly improved, and the brown planthopper-resistant rice variety (line) containing Bph56 is obtained.

The present application provides a brown planthopper lethal gene Nlpfam and its application in biological control. After the brown planthopper lethal gene Nlpfam is interfered with, it can cause the death of brown planthoppers. Therefore, by transcribing the brown planthopper lethal gene Nlpfam to prepare dsRNA or an interfering reagent, injecting it into brown planthoppers can effectively interfere with the Nlpfam gene, causing the abdomen of brown planthoppers to turn black, effectively killing brown planthoppers, and achieving biological control of brown planthoppers.

The invention belongs to the technical field of application of miR-3032-z in preparation of a product for inhibiting expression of a brown planthopper CYP15A1 gene, and particularly relates to the technical field of pest control. The invention provides an application of miR-3032-z or a reagent for overexpressing miR-3032-z in preparation of a product for inhibiting the expression of a CYP15A1 gene of brown planthopper. The products include medicaments or kits. The miR-3032-z is combined with a target gene CYP15A13 'UTR region in a targeted manner to regulate the expression of the gene, the overexpression of the miR-3032-z can inhibit the expression of a cytochrome P450 family gene CYP15A1, the honeydew secretion amount and insect weight gain of biological type Y brown planthoppers on insect-resistant rice YHY15 can be remarkably reduced, the feeding behavior of the biological type Y brown planthoppers can be remarkably inhibited, and the sensitivity of harmful type brown planthoppers to the resistant rice YHY15 is recovered.

The invention discloses a brown planthopper gene NlCYP303A1 and a nano nucleic acid pesticide of the brown planthopper gene NlCYP303A1. The nucleotide sequence of the gene NlCYP303A1 disclosed by the invention is as shown in SEQ ID NO: 1; the nano nucleic acid pesticide is mainly prepared from zinc nitrate hexahydrate, dsN1CYP303A1 and 2-methylimidazole. The gene NlCYP303A1 closely related to the ecdysis process of the brown planthopper is identified, and silencing of the gene can cause ecdysis failure of the brown planthopper and reduce the survival rate of the brown planthopper; furthermore, a dsN1CYP303A1-coated ZIF-8 nano nucleic acid pesticide delivery system is constructed on the basis of a nano carrier ZIF-8, and the biological activity of the dsN1CYP303A1-coated ZIF-8 nano nucleic acid pesticide delivery system is measured; results show that the nano nucleic acid pesticide delivery system overcomes the defect that the service life of dsRNA is short, and the control effect of the nano nucleic acid pesticide delivery system on brown planthopper is remarkably improved. The potential application value of RNAi in pest control is improved, and a new strategy is provided for pest drug resistance treatment.

The present invention provides the use of 1-methoxyindole or its derivatives in repelling and / or killing pests, belonging to the field of pest control technology. The active ingredient provided by the present invention is 1-methoxyindole (1-MOI), which is found in Metarhizium robertsiense. When applied as a repellent, the concentration in a plane is 0.127 μg / cm 2 1-methoxyindole can repel a variety of ants, termites, and ticks, and the higher the 1-methoxyindole content, the stronger the repellent effect; when used as an insecticide, the concentration in the air is 0.14μg / cm 3 The 1-methoxyindole can kill a variety of ants, termites, ticks, thrips, mosquitoes, as well as whiteflies, brown planthoppers, spider mites and other pests, and the higher the concentration of 1-methoxyindole, the stronger the killing effect.

To provide an inhibitor for suppressing expression of an antibacterial peptide in brown planthopper and an application thereof.SOLUTION: A dsNlPCE4 inhibitor designed based on the NlPCE4 gene of the brown planthopper is obtained. The inhibitor, when applied to brown planthoppers inoculated on their body surface with Metarhizium anisopliae, significantly increases the mortality of the brown planthopper. The present invention combines two technical strategies for pest control, biocontrol fungi and RNA interference (RNAi), to provide a new target for brown planthopper control technology based on cooperative mediation of RNAi.SELECTED DRAWING: Figure 5

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention discloses application of nilaparvata lugens NlGRP protein in prevention and treatment of nilaparvata lugens, and belongs to the technical field of prevention and treatment of nilaparvata lugens. By inhibiting the expression of the coding gene of the NlGRP protein in the brown planthopper, the growth and development of the brown planthopper can be obviously inhibited. Wide application prospects are realized; the method has great application value for reducing pesticide use, maintaining ecological balance and realizing sustainable development.

The invention discloses a specific saliva gene NlSP55 of brown planthopper and application of the specific saliva gene NlSP55 in biological control of brown planthopper. Wherein the nucleotide sequence of the nilaparvata lugens saliva gene NlSP55 is as shown in SEQ ID NO. 1, and the coded amino acid sequence is as shown in SEQ ID NO. 2. The dsRNA of the synthesized saliva gene NlSP55 is introduced into the brown planthopper body by using an RNA interference technology, so that the NlSP55 gene can be silent in a targeted manner, the feeding capacity of the brown planthopper is inhibited, the survival rate of the brown planthopper on sensitive rice and resistant rice is quickly reduced, and finally, the biological control effect of the brown planthopper is achieved. The invention specifically aims at the brown planthopper, is harmless to mammals, fishes and shrimps, natural enemy insects and pollination insects, and provides a new way for preventing and treating the brown planthopper.

The invention discloses an agent for delaying development of drug resistance of brown planthopper and application thereof.The agent comprises 96.6% of pymetrozine original medicine and 12% of Zhongshengmycin original medicine, and the 96.6% of pymetrozine original medicine and the 12% of Zhongshengmycin original medicine are prepared through an organic solvent N, N-dimethyl formamide. The preparation method comprises the following steps: dissolving N, N-dimethylformamide analytically pure (DMF), diluting with distilled water to obtain a pymetrozine solution with the LC50 value of 3.80 mg / L and a zhongshengmycin solution with the LC50 value of 152.61 mg / L, and mixing the pymetrozine solution with the LC50 value of 3.80 mg / L and the zhongshengmycin solution with the LC50 value of 152.61 mg / L according to a volume ratio to prepare the medicament. The invention aims to provide an agent for preventing and treating brown planthopper and application of the agent for delaying development of drug resistance of brown planthopper, aiming at the defect that the chemical prevention and treatment effect is obviously reduced due to drug resistance of brown planthopper to conventional chemical insecticides.

This invention discloses the use of a DGAT1 gene and its dsRNA in the preparation of drugs for controlling brown planthoppers. This invention demonstrates that inhibiting the expression of the DGAT1 gene in brown planthoppers can significantly reduce the survival rate of brown planthoppers, thereby effectively controlling the brown planthopper population. This invention utilizes RNA interference (RNAi) technology to inhibit the expression of the DGAT1 gene in brown planthoppers. This invention provides a new strategy and target site for controlling brown planthoppers, making RNAi technology for inhibiting the expression of the DGAT1 gene in brown planthoppers broadly applicable in this field.

The invention discloses application of an F-box gene OsFBX49 to brown planthopper resistance of rice, and belongs to the technical field of plant genetic engineering. The amino acid sequence of a protein encoded by the gene OsFBX49 is as shown in SEQ ID NO. 1. According to the invention, through agrobacterium-mediated genetic transformation, the OsFBX49 gene is transferred into insect-sensitive rice, and the result shows that the resistance of a plant over-expressing the OsFBX49 gene to brown planthopper is enhanced. After the brown planthopper eats the transgenic plant, the survival rate of the brown planthopper is obviously reduced, the weight of the brown planthopper is increased, and the amount of secreted honeydew is obviously reduced. The gene provided by the invention provides a basis for research on interaction of rice and brown planthopper genes, and has reference significance for research on gene molecular functions and breeding.

The present invention provides an isoxazoline-substituted benzamide compound and a stereoisomer and agriculturally or veterinarily acceptable salt thereof. The compound is as represented by formula (I). In the formula, X represents hydrogen, halogen, hydroxyl, alkoxy, or alkylthio; Y is hydrogen, halogen, alkyl, or haloalkyl; Z is haloalkyl or haloalkoxy; M is CH or N; Q is hydrogen or halogen; R1 is hydrogen, halogen, hydroxyl, cyano, amino, nitro, formyl, cyanoalkyl, hydroxyalkyl, alkyl, etc.; and R2 is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, etc. The compound has an excellent control effect on pests such as Spodoptera frugiperda, Myzus persicae, Nilaparvata lugens, Tetranychus truncates, and Bradysia odoriphaga.

The invention belongs to the field of biological prevention and control, and discloses an insect specific virus NLBV3 and application thereof in reducing transmission of rice odontodwarf virus of brown planthopper. The NLBV3 virus genome comprises three chains, the L chain encodes RNA dependent RNA polymerase (RdRP), the M chain encodes glycoprotein precursor (GP), the S chain encodes nucleoprotein (NP), and the nucleotide sequences are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; the application is realized by releasing the NLBV3-carrying brown planthopper population, and according to the screening and detection method of the NLBV3-carrying brown planthopper high-virus population provided by the invention, natural brown planthoppers can be effectively infected after the NLBV3-carrying brown planthopper high-virus population is released, the RRSV transmission capability of the brown planthoppers is reduced, and the virus is prevented from damaging rice production. The NLBV3 provided by the invention is an insect specific virus, is harmless to mammals, fishes and shrimps, natural enemy insects and pollination insects, and has the advantages of specific action object, environment friendliness and the like.

The invention provides application of brown planthopper NlP21 protein and a coding gene thereof in regulation and control of brown planthopper resistance of plants, and belongs to the technical field of gene engineering. The invention provides a brown planthopper NlP21 protein and a sequence of a coding gene thereof. The gene is of great significance in regulating and controlling the resistance of plants to brown planthoppers and regulating and controlling the growth of the brown planthoppers. The viability of the brown planthopper can be effectively reduced by inhibiting the expression of the NlP21 gene in the brown planthopper, and the resistance of the plant to the brown planthopper can be effectively improved by overexpressing the NlP21 gene in the plant. The nilaparvata lugens NlP21 protein and the coding gene thereof provided by the invention have good application prospects, and have great application values for reducing pesticide use, maintaining ecological balance and realizing sustainable development.

The invention discloses brown planthopper resistant dominant complementary genes Bph50 and Bph51 as well as an encoding protein and application thereof, and belongs to the technical field of biology. The amino acid sequence of the protein coded by the Bph50 gene is as shown in SEQ ID NO. 2, and the nucleotide sequence of the Bph50 gene is as shown in SEQ ID NO. 1. The amino acid sequence of the protein coded by the Bph51 gene is as shown in SEQ ID NO.4, and the nucleotide sequence of the Bph51 gene is as shown in SEQ ID NO.3. The invention provides a novel brown planthopper resistance gene and a brown planthopper resistance identification method aiming at the gene, and a foundation is laid for the cultivation of a novel brown planthopper resistant rice variety. According to the invention, the strain Xinxiangzhan No.1 which is high in yield, rich in fragrance and good in brown planthopper resistance is obtained by culturing BPHR96 containing dominant complementary genes Bph50 and Bph51 resistant to brown planthoppers.

The invention discloses application of a rice brown planthopper resistant gene OsCYT2 in rice brown planthopper resistance, and relates to the technical field of brown planthopper resistant transgenic rice. According to the invention, through agrobacterium-mediated genetic transformation, the OsCYT2 gene is transferred into an insect-susceptible rice variety Nipponbare, and the resistance of a plant over-expressed with the OsCYT2 gene to brown planthopper is enhanced. After the brown planthopper eats the transgenic plant, the survival rate of the brown planthopper is reduced, the weight gain of the brown planthopper is reduced, and the amount of secreted honeydew is reduced. The new insect-resistant function of the gene OsCYT2 found by the invention has reference significance for researching gene molecular functions and breeding.

The invention discloses a sialoprotein gene for regulating and controlling the growth and development of brown planthoppers as well as dsRNA and application thereof. The nucleotide sequence of the sialoprotein gene SISP is shown as SEQ ID NO.1; the dsRNA sequence of the sialoprotein gene for regulating and controlling the growth and development of the brown planthopper is as shown in SEQ ID NO.3. According to the dsRNA disclosed by the invention, SISP can be effectively silenced, the dsRNA is prepared into a nucleic acid pesticide to be applied to brown planthopper, the inhibition of the brown planthopper on the insect resistance of rice can be reduced, and the dsRNA has a serious adverse effect on normal feeding, survival and reproduction of the brown planthopper. The method has the advantages of no pollution and reduction of the use amount of chemical pesticides. Besides, the SISP gene is overexpressed in the rice, so that the content of lignin and flavonoid compounds in the rice can be remarkably reduced, and the insect resistance of the rice is reduced.

The present invention belongs to the field of plant genetic engineering technology, and in particular to a rice cytokinin response regulator gene OsRR24 And application, the rice cytokinin response regulatory factor gene OsRR24 It encodes a protein containing a DNA binding domain and a conserved aspartate-containing signal receiving domain, and is a response regulator downstream of the rice CK signaling pathway. OsRR24 During the functional process in rice, it was transferred into Nipponbare and Bph6 In the NIL, overexpressing plants showed significantly enhanced resistance to brown planthoppers, while RNAi-inhibited plants showed significantly reduced resistance to brown planthoppers. The gene of this invention provides a good theoretical basis for studying how cytokinins participate in rice insect resistance through response regulatory factors. It has reference significance for studying gene molecular functions and also provides genetic resources for the design and breeding of insect-resistant rice varieties.

The present application relates to the field of biological genetic engineering technology, in particular to the application of silencing miR529b in the positive regulation of rice resistance to brown planthopper. The silencing vector of miR529b is obtained by short tandem target mimic (STTM) technology, which silences miR529b in the receptor rice, and obtains the transgenic plant STTM-miR529b with silenced miR529b. The T2 generation homozygous transgenic plants are identified for brown planthopper resistance by seedling group method. Compared with the transgenic receptor parent Nipponbare (control group, marked as NIP, same below), STTM-miR529b is more resistant to pests, which confirms that miR529b is related to the resistance of rice to brown planthopper, and can be used for creating new germplasm of rice resistance to brown planthopper.

The invention provides a molecular marker primer closely linked with a brown planthopper resistant major gene Bph48 of rice as well as a marking method and application of the molecular marker primer. A rice insect-resistant variety 75-9-3 (called C268 for short) and Yangdao 9 (called 9311 for short) are hybridized to obtain genotypes of F2 individual plants, genetic linkage analysis is performed in combination with the brown planthopper resistance level of an F3 family, a resistance major gene Bph48 carried by the insect-resistant variety C268 is identified, a Bph48 positioning region is reduced to a 370kb fragment limited by markers 1M30.093 and 1M30.471 on the two sides, and the Bph48 gene is identified to be a Bph48 gene. And the molecular marker is closely linked with the molecular marker 1M30.471. The molecular marker 1M30.471 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C268 and derived varieties (lines) thereof contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper resistant character is greatly improved, and the brown planthopper resistant rice variety (line) containing Bph48 is obtained.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker of an anti-brown planthopper major gene locus Bph33 of a rice variety W41123 and application of the SNP molecular marker. The invention discloses an SNP (Single Nucleotide Polymorphism) marker KBPH33 which is closely linked with a rice brown planthopper resistant gene locus Bph33 and has good amplification efficiency. The marker detection is located at the 19571017 site of the No.4 chromosome of Nipponbare rice, the polymorphism is A / G, and a marker primer developed on the basis of a KASP technology is as shown in SEQ ID No: 1-3. The SNP marker disclosed by the invention can be used for detecting a close linkage site of a Bph33 site, can be conveniently and efficiently used for efficiently identifying whether W41123 and derived varieties thereof contain the major gene site or not, can predict the resistance level of brown planthopper in a period, and greatly improves the breeding efficiency.

The invention discloses a rice highly resistant brown planthopper mutant arc3 and its application, and the preparation method thereof specifically comprises the following steps: screening the sgRNA target sequence of the rice gene Os03g50150; designing primers as shown in SEQ ID NO.2 and SEQ ID NO.3 according to the sgRNA target sequence; mixing the primers and performing annealing treatment to form double-stranded DNA; first constructing the double-stranded DNA fragment onto a pOs-sgRNA vector, and then transferring it onto a pH-Ubi-cas9 vector; transferring the vector into rice tissue for induction and obtaining a rice resistant brown planthopper mutant arc3 plant through genetic transformation. In the invention, the expression or activity of the Os03g50150 gene or the active fragment of the Os03g50150 gene in rice is reduced; and the resistance of rice to brown planthopper is significantly improved.