27 results about "Brown planthopper" patented technology

This invention relates to biological control, and in particular to a method NlVHA- A Application and methods of dsRNA in the control of brown planthoppers. This invention clones the V-ATPase gene of the brown planthopper. NlVHA-A The encoded protein sequence was obtained through in vitro synthesis. NlVHA-A The dsRNA of the gene was injected into the brown planthopper. This silenced the brown planthopper. NlVHA-A Genes can reduce the survival rate of brown planthoppers, the honeydew production and weight gain of emerging females, thereby reducing the direct impact of brown planthoppers on rice and achieving the goal of prevention and control.

This invention discloses the application of the OsSMP3 gene in the control of rice brown planthopper, specifically disclosing that the OsSMP3 gene (LOC_Os11g05170) negatively regulates rice resistance to brown planthopper. By targeting this gene using CRISPR / Cas9 gene editing technology, a loss-of-function mutant was obtained. Compared to the wild type, the knockout mutant showed significantly enhanced resistance to brown planthopper, specifically manifested in increased rice plant survival rate. This invention provides new gene resources and targets for molecular breeding of rice resistant to brown planthopper.

The application discloses a gene for regulating brown planthopper resistance of rice METS2 and application thereof, and belongs to the technical field of genetic engineering. METS2 The CDS sequence of the gene is shown as SEQ ID NO. 3. Overexpression of the gene can significantly improve the resistance of rice to the brown planthopper, and provides important gene resources and technical support for rice pest resistance breeding. METS2 The gene overexpression type transgenic rice can be used as a new rice germplasm material, and is used for in-depth research on the brown planthopper resistance mechanism of rice, and for excavation of more genes for regulating the brown planthopper resistance, and has important application value for effectively improving the brown planthopper resistance of rice by means of genetic breeding and genetic engineering methods.

This invention provides a rice gene Bph54 for resistance to brown planthopper and its application. The rice provided by this invention... Bph54 Genes are of great significance for breeding new rice varieties resistant to brown planthoppers. This invention successfully cloned the rice Bph54 gene using map-based cloning, and performed functional annotation and identification. This gene exhibits complete dominance, and both homozygous and heterozygous genotypes confer equal resistance to brown planthoppers in rice. This characteristic is beneficial for the application of this gene in breeding. Based on this, the Bph54 gene can be introduced into brown planthopper-susceptible plant varieties through traditional breeding techniques such as hybridization and backcrossing, or through genetic engineering, to cultivate new varieties of plants resistant to brown planthoppers with stable genetic traits. This invention provides important germplasm resources for effectively reducing insect damage and achieving increased and stable crop yields.

This invention provides the application of the NlP21 protein and its encoding gene in regulating plant resistance to brown planthoppers, belonging to the field of genetic engineering technology. This invention provides the sequence of the NlP21 protein and its encoding gene in brown planthoppers. This gene is of great significance for regulating plant resistance to brown planthoppers and regulating the growth of brown planthoppers. Inhibiting the expression of the NlP21 gene in brown planthoppers can effectively reduce their survival ability, while overexpressing the NlP21 gene in plants can effectively improve plant resistance to brown planthoppers. The NlP21 protein and its encoding gene provided by this invention have excellent application prospects and significant application value for reducing pesticide use, maintaining ecological balance, and promoting sustainable development.

The application belongs to the field of agricultural biotechnology, and particularly relates to a dsRNA of a brown planthopper gene and application of the dsRNA in preventing and treating the brown planthopper and related methods. NlUGT7 The dsRNA of the gene is prepared through in vitro transcription, and RNA interference treatment is performed on the brown planthopper through microinjection. NlUGT7 The results show that after the dsRNA is injected, the expression amount of the gene in the brown planthopper of biotype Y and biotype 1 is significantly reduced. NlUGT7 After the gene is silenced, the survival rate of the brown planthopper of biotype Y feeding on YHY15 rice containing a Bph15 resistance gene or the brown planthopper of biotype 1 feeding on TN1 rice not containing the resistance gene is significantly reduced. NlUGT7 The gene plays an important role in the survival process of the brown planthopper, and can be used as an effective target for preventing and treating the brown planthopper. NlUGT7 The gene and the dsRNA thereof provided by the application can be applied to research and development of biological pesticides for the brown planthopper and green prevention and control, and have good application prospects.

This invention belongs to the field of biological control technology for brown planthoppers, specifically relating to the application of the Tip60 gene and its encoded protein in the control of brown planthoppers. This invention is the first to discover that the Tip60 gene plays a crucial role in the control of brown planthoppers. Further experiments confirmed that reducing the expression level of the Tip60 gene significantly shortens the survival time of female brown planthoppers and reduces the number of offspring, indicating that inhibiting Tip60 gene expression can effectively control brown planthoppers, providing a theoretical basis for this strategy. Simultaneously, this invention uses RNAi technology to interfere with the expression of this gene during the adult stage of brown planthoppers, producing a significant inhibitory effect, thus providing a novel strategy and target for brown planthopper control, and demonstrating the application prospects of RNAi technology in silencing the Tip60 gene in brown planthopper control.

This invention relates to the field of agricultural technology, specifically to a mutant rice OsDSP1 gene and its applications. Compared to the wild-type rice OsDSP1 gene, the mutant OsDSP1 gene has a T base inserted after position 115 or a G base deleted at position 116. The mutant rice OsDSP1 gene provided by this invention can improve rice resistance to brown planthoppers, while also increasing grain length and thousand-grain weight, effectively increasing rice yield. This invention provides new gene resources and improvement targets for the breeding of insect-resistant rice varieties, and has significant application value for insect-resistant and yield-increasing plant breeding.

The application discloses a breeding method of a salt-tolerant multi-resistance high-quality rice restorer line. A restorer line resistant to bacterial leaf blight, rice blast and brown planthopper is used as a female parent, a strong salt-tolerant germplasm is used as a male parent, and then F2 generation is obtained after self-crossing; 0‰, 3‰ and 5‰ salt gradient stress is carried out on the F2 generation under stable salt environment conditions, single plants with low glume degeneration rate and good plant leaf type are screened, and the single plants are crossed with Yuzhenxiang; the cross progeny is identified through multi-generation salt stress, the agronomic characters, resistance and rice quality of the cross progeny are screened under a non-salt environment, and salt-tolerant, multi-resistance, high-quality and fragrance characters are aggregated; the cross progeny is verified through natural salt stress in a coastal saline field, and a salt-tolerant multi-resistance high-quality rice restorer line with a relative yield loss rate of less than 40% in a 5‰ salt concentration, resistance to bacterial leaf blight, rice blast and brown planthopper, a grain length-width ratio greater than 4.0, a chalky grain rate lower than 7.0% and fragrance is obtained. The application integrates multi-gradient salt stress identification, intergenerational cross selection and multi-character aggregation, and creates the salt-tolerant multi-resistance high-quality rice restorer line.

This invention belongs to the field of pesticide technology, specifically relating to a triazole compound, its preparation method, insecticidal composition, and application. The compound is shown in general formula I: wherein X represents O or S; A1 represents N or CH; A2 and A3 independently represent N or CR5, and only one of A1, A2, and A3 is N; R1 represents alkyl, etc.; R2, R3, R5, R6, and R8 independently represent hydrogen, halogen, alkyl, alkenyl, etc.; R4 and R7 independently represent hydrogen, alkyl, etc. The compound or its salts exhibit excellent control effects against brown planthoppers, cereal aphids, gray planthoppers, white-backed planthoppers, peach aphids, and peanut aphids.

This invention provides a molecular marker primer tightly linked to the major brown planthopper resistance gene Bph57 in rice, along with its marking method and application. This invention utilizes genetic linkage analysis of individual F2 plants obtained from crossing the insect-resistant rice variety “Daomushe Late Bengu” (C9) (♂) with 9311 (♀), combined with the brown planthopper resistance levels of F3 families. This analysis identifies the major resistance gene Bph57 carried by the insect-resistant variety C9. The Bph57 region is narrowed down to an approximately 90kb fragment defined by flanking markers 2M26.442 and 2M26.536, and tightly linked to molecular marker 2M26.762. The molecular marker 2M26.762 of this invention effectively detects whether the insect-resistant variety C9 and its derived varieties (lines) contain this major resistance gene locus, significantly improving the selection efficiency for brown planthopper resistance and obtaining brown planthopper-resistant rice varieties (lines) containing Bph57.

The application discloses a kind of Nilaparvata lugens NlCP6 proteins and its coding gene in the application of regulating plant resistance to Nilaparvata lugens, it is related to the genetic engineering prevention and control technical field of rice Nilaparvata lugens.The application can significantly inhibit the growth and development of Nilaparvata lugens by inhibiting the expression of the coding gene of NlCP6 protein in Nilaparvata lugens;in view of the overexpression of Nilaparvata lugens NlCP6 gene, the overexpression vector of the gene is constructed and is transferred into rice plant, so that the resistance of the plant to Nilaparvata lugens is greatly improved, and the weight gain and survival rate of Nilaparvata lugens after feeding the transgenic plant are significantly reduced.The application method provided by the application shows that Nilaparvata lugens NlCP6 gene plays a very important role in improving the Nilaparvata lugens resistance of rice and inhibiting the growth and development of Nilaparvata lugens, and has wide application prospect;it has great application value for reducing pesticide use, maintaining ecological balance and sustainable development.

This invention belongs to the field of agricultural nanoparticle formulations and green pest control technology, specifically relating to an insecticide nano-synergist Cu-TA, its preparation method, and its application. The Cu-TA consists of nanoparticles formed by the coordination assembly of copper ions and tannic acid. The Cu-TA can be used in combination with various insecticides to increase the sensitivity of brown planthoppers, white-backed planthoppers, cotton aphids, rice stem borers, and common thrips to pesticides, and to improve the field control effect on brown planthoppers. Furthermore, the combined use of Cu-TA and acetamiprid can reduce the actual heritability of resistance to acetamiprid in brown planthoppers, thereby delaying the development of resistance.

The present application belongs to the field of agricultural biotechnology, and relates to application and method of Nilaparvata lugens NlUGT4 gene in preventing and treating Nilaparvata lugens pest. The present application provides nucleotide and amino acid sequences of Nilaparvata lugens NlUGT4 gene, and can significantly reduce the survival rate of Nilaparvata lugens by silencing NlUGT4 gene through RNA interference technology. The present application has good killing effect on Nilaparvata lugens, and has good application prospect.

The application discloses a pesticide composition containing annonaceous acetogenins and triflumuron and application thereof, and belongs to the technical field of pesticides. Effective components of the composition are the mass ratio of 1:10 to 20:1 of the annonaceous acetogenins and the triflumuron, wherein the annonaceous acetogenins are ortho-bis-tetrahydrofuran lactones extracted from seeds of Annona squamosa, and the purity is greater than or equal to 90%. The total mass percentage content of the effective components in the composition is 10% to 30%, and the rest is a pesticide adjuvant. The composition can be prepared into various dosage forms such as suspending agents, emulsifiable concentrates, suspending seed-coating agents, soluble granules or micro-capsule suspending-suspending agents. The two effective components with different action mechanisms are scientifically compounded, and the composition shows a significant synergistic effect on piercing-sucking mouthpart pests such as wheat aphids, corn aphids and brown planthoppers, and the highest co-toxicity coefficient (CTC) can reach 176.31. The composition can effectively reduce the field chemical pesticide dosage, delay the development of pest resistance and reduce pesticide residues, and has the characteristics of high efficiency, low residue and environmental friendliness.

This invention belongs to the fields of plant genetic engineering and agricultural biotechnology, and discloses the application of the OsCCR21, OsPAL05, OsPAL1, OsPAL07, and 4CL1 genes in regulating the rice phyllosphere microbiome and resistance. The nucleotide sequence of the OsCCR21 gene is shown in SEQ ID NO:1, the OsPAL05 gene in SEQ ID NO:2, the OsPAL1 gene in SEQ ID NO:3, the OsPAL07 gene in SEQ ID NO:4, and the 4CL1 gene in SEQ ID NO:5. This invention reveals that the OsCCR21, OsPAL07, OsPAL05, OsPAL1, and 4CL1 genes positively regulate the abundance of specific beneficial microbial orders (Pseudomonas or Burkholderia) in the rice phyllosphere, thereby enhancing the resistance of rice to different stresses such as sheath blight, brown planthopper, and rice blast.

The application belongs to the technical field of biological control, and particularly relates to a gene fragment Nlug001825.1 of a brown planthopper and application of dsRNA of the gene fragment in preventing and treating the brown planthopper. The application provides the gene fragment Nlug001825.1 capable of causing death of the brown planthopper after interference and dsRNA of the gene fragment. The results of examples show that the dsRNA can effectively kill the brown planthopper after injection. The method for preventing and treating the brown planthopper provided by the application is friendly to the environment ecology and food safety, has the application value of green safety, and establishes a new way for controlling pests by using the RNA interference technology.

This invention discloses a rice gene for resistance to brown planthoppers. WRKY51 Its applications belong to the field of genetic engineering technology. WRKY51 The CDS sequence of the gene is shown in SEQ ID NO.3. This invention utilizes overexpression in rice... WRKY51 Genes can significantly improve rice resistance to brown planthoppers, providing new genetic resources and technical support for molecular breeding of rice resistant to brown planthoppers, helping to cultivate new rice varieties resistant to brown planthoppers, and promoting green pest control and safe production of rice.

This invention discloses a method for altering the reproductive pattern of *Trichogramma pseudooligophthalmus*. The method includes treating *Trichogramma pseudooligophthalmus* that is fully engaged in parthenogenesis with a rifampicin-honey aqueous solution at a concentration of 0.1-0.5 mg / ml. After treatment, the *Trichogramma pseudooligophthalmus* is used to parasitize brown planthopper eggs to obtain male F1 generation *Trichogramma pseudooligophthalmus*. This invention provides a model for exploring the reproductive methods of *Wolbachia* in hymenoptera, lays the foundation for further exploration of the reproductive patterns of *Trichogramma pseudooligophthalmus*, and also provides a model for the removal of endosymbiotic bacteria in other arthropods.

The application discloses application and method of rice gene OsPIE in improving rice insect resistance. The gene is a DNA sequence of SEQ ID No. 1, and encodes a protein with 576 amino acid residues. Research finds that the gene is closely related to rice insect resistance, and overexpression of the OsPIE gene in rice can effectively improve direct resistance of the rice to brown planthopper. The application will be widely applied in crop pest control and insect-resistant crop breeding, especially in rice pest control and insect-resistant rice breeding.

The present disclosure provides an application of ENO gene in improving resistance to brown planthopper and rice with resistance to brown planthopper, belonging to the field of biotechnology. The application of the ENO gene in improving resistance to brown planthopper includes: using the ENO gene to improve resistance to brown planthopper. The rice with resistance to brown planthopper contains a sequence as shown in SEQ ID NO: 1 in the sequence table. The present disclosure uses the importance of ENO in plants, uses the ENO gene to improve resistance to brown planthopper, and the recipient plant can effectively improve the resistance of rice to brown planthopper, and the effect is significant.

The present invention belongs to the technical field of pesticides, and specifically relates to a triazole compound and a preparation method therefor, and an insecticidal composition and the use thereof. The compound is as represented by general formula I, where X represents CR11 or N; X2 represents CR2 or N; W represents O or S; R1, R2, R3, R4, R5, R6, R7, R9 and R11 each independently represents hydrogen, halogen, alkyl, etc.; R8 represents hydrogen, alkyl, alkenyl, alkynyl, etc.; and R10 represents hydrogen, alkyl, alkenyl, alkynyl, etc. The compound exhibits a good control effect on Nilaparvata lugens, Rhopalosiphum padi, Laodelphax striatellus, Sogatella furcifera, Myzus persicae, Aphis craccivora, etc.

This invention belongs to the field of biotechnology, specifically relating to the brown planthopper. NIVHA-c Application and methods of genes and their dsRNA in the control of brown planthoppers. This was achieved through the preparation of... NIVHA-c RNAi treatment of the brown planthopper with the gene's dsRNA revealed that silencing this gene significantly reduced the planthopper's survival rate, and markedly decreased honeydew secretion and weight gain in adults. L4440- was constructed. NIVHA-c The dsRNA expression vector was transformed into *E. coli*, and dsRNA was produced after IPTG induction. The survival rate of brown planthoppers that fed on artificial feed containing dsRNA also decreased significantly; indicating that... NIVHA-c The gene plays an important role in the growth, development, and feeding process of the brown planthopper, and its dsRNA has a good pest control effect. This invention provides... NIVHA-c NIVHA-c Genes and their dsRNAs can serve as important targets for the development of biopesticides and the biological control of brown planthoppers, which is of great significance for the green control of brown planthoppers.

This invention belongs to the field of pest control technology, specifically relating to the application of nocaketone as an attractant for rice brown planthoppers and the attractant itself. The attractant is a solution containing the active ingredient of nocaketone, a natural and safe ingredient. In this invention, the application of nocaketone as an attractant for rice brown planthoppers can be achieved by directly spraying the attractant onto rice plants, utilizing the volatility of nocaketone, or by loading it onto a slow-release carrier to create a lure, which then releases nocaketone to attract rice brown planthoppers. Verification by this invention shows that nocaketone can serve as a "luring trap" for the green control of brown planthoppers, reducing the egg-laying and feeding rates of female adults by attracting them, thus producing a good preventative effect and providing a new direction for the green control of brown planthoppers.