144 results about "Brown planthopper" patented technology

The invention provides a closely linked molecular marker primer of a rice brown planthopper resistant major gene Bph56 as well as a marking method and application of the closely linked molecular marker primer. According to the invention, genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety C127 (or) and KW (or) are combined with brown planthopper resistance levels of an F3 family for genetic linkage analysis, a resistance major gene Bph56 carried by the insect-resistant variety C127 is identified, and a region of the resistance major gene Bph56 is reduced to a 77kb fragment limited by markers 6M26.278 and 6M26.355 on two sides and is closely linked with a molecular marker 6M26.355. The molecular marker 6M26.355 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C127 and derived varieties (lines) of the insect-resistant variety C127 contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper-resistant character is greatly improved, and the brown planthopper-resistant rice variety (line) containing Bph56 is obtained.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention belongs to the field of biological prevention and control, and discloses an insect specific virus NLBV3 and application thereof in reducing transmission of rice odontodwarf virus of brown planthopper. The NLBV3 virus genome comprises three chains, the L chain encodes RNA dependent RNA polymerase (RdRP), the M chain encodes glycoprotein precursor (GP), the S chain encodes nucleoprotein (NP), and the nucleotide sequences are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; the application is realized by releasing the NLBV3-carrying brown planthopper population, and according to the screening and detection method of the NLBV3-carrying brown planthopper high-virus population provided by the invention, natural brown planthoppers can be effectively infected after the NLBV3-carrying brown planthopper high-virus population is released, the RRSV transmission capability of the brown planthoppers is reduced, and the virus is prevented from damaging rice production. The NLBV3 provided by the invention is an insect specific virus, is harmless to mammals, fishes and shrimps, natural enemy insects and pollination insects, and has the advantages of specific action object, environment friendliness and the like.

The invention discloses brown planthopper resistant dominant complementary genes Bph50 and Bph51 as well as an encoding protein and application thereof, and belongs to the technical field of biology. The amino acid sequence of the protein coded by the Bph50 gene is as shown in SEQ ID NO. 2, and the nucleotide sequence of the Bph50 gene is as shown in SEQ ID NO. 1. The amino acid sequence of the protein coded by the Bph51 gene is as shown in SEQ ID NO.4, and the nucleotide sequence of the Bph51 gene is as shown in SEQ ID NO.3. The invention provides a novel brown planthopper resistance gene and a brown planthopper resistance identification method aiming at the gene, and a foundation is laid for the cultivation of a novel brown planthopper resistant rice variety. According to the invention, the strain Xinxiangzhan No.1 which is high in yield, rich in fragrance and good in brown planthopper resistance is obtained by culturing BPHR96 containing dominant complementary genes Bph50 and Bph51 resistant to brown planthoppers.

The present application relates to the field of biological genetic engineering technology, in particular to the application of silencing miR529b in the positive regulation of rice resistance to brown planthopper. The silencing vector of miR529b is obtained by short tandem target mimic (STTM) technology, which silences miR529b in the receptor rice, and obtains the transgenic plant STTM-miR529b with silenced miR529b. The T2 generation homozygous transgenic plants are identified for brown planthopper resistance by seedling group method. Compared with the transgenic receptor parent Nipponbare (control group, marked as NIP, same below), STTM-miR529b is more resistant to pests, which confirms that miR529b is related to the resistance of rice to brown planthopper, and can be used for creating new germplasm of rice resistance to brown planthopper.

The invention provides a molecular marker primer closely linked with a brown planthopper resistant major gene Bph48 of rice as well as a marking method and application of the molecular marker primer. A rice insect-resistant variety 75-9-3 (called C268 for short) and Yangdao 9 (called 9311 for short) are hybridized to obtain genotypes of F2 individual plants, genetic linkage analysis is performed in combination with the brown planthopper resistance level of an F3 family, a resistance major gene Bph48 carried by the insect-resistant variety C268 is identified, a Bph48 positioning region is reduced to a 370kb fragment limited by markers 1M30.093 and 1M30.471 on the two sides, and the Bph48 gene is identified to be a Bph48 gene. And the molecular marker is closely linked with the molecular marker 1M30.471. The molecular marker 1M30.471 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C268 and derived varieties (lines) thereof contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper resistant character is greatly improved, and the brown planthopper resistant rice variety (line) containing Bph48 is obtained.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker of an anti-brown planthopper major gene locus Bph33 of a rice variety W41123 and application of the SNP molecular marker. The invention discloses an SNP (Single Nucleotide Polymorphism) marker KBPH33 which is closely linked with a rice brown planthopper resistant gene locus Bph33 and has good amplification efficiency. The marker detection is located at the 19571017 site of the No.4 chromosome of Nipponbare rice, the polymorphism is A / G, and a marker primer developed on the basis of a KASP technology is as shown in SEQ ID No: 1-3. The SNP marker disclosed by the invention can be used for detecting a close linkage site of a Bph33 site, can be conveniently and efficiently used for efficiently identifying whether W41123 and derived varieties thereof contain the major gene site or not, can predict the resistance level of brown planthopper in a period, and greatly improves the breeding efficiency.

The invention belongs to the technical field of pesticides, and particularly relates to a heterocyclic substituted pyridine compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, wherein R1, R2, R4, R5, R6, R8, R9 and R10 respectively and independently represent hydrogen, halogen, alkyl, alkenyl and the like; r3 represents hydrogen, an alkyl group, a haloalkyl group, an alkenyl group or the like; and R7 represents halogen. The compound has excellent prevention and treatment effects on spodoptera frugiperda, armyworm, prodenia litura, beet armyworm, agrotis ypsilon, chilo suppressalis, plutella xylostella, brown planthopper, rhopalosiphum padi and the like.

This invention relates to biological control, and in particular to a method NlVHA- A Application and methods of dsRNA in the control of brown planthoppers. This invention clones the V-ATPase gene of the brown planthopper. NlVHA-A The encoded protein sequence was obtained through in vitro synthesis. NlVHA-A The dsRNA of the gene was injected into the brown planthopper. This silenced the brown planthopper. NlVHA-A Genes can reduce the survival rate of brown planthoppers, the honeydew production and weight gain of emerging females, thereby reducing the direct impact of brown planthoppers on rice and achieving the goal of prevention and control.

The present disclosure provides an application of a CE1 gene in improving resistance to brown planthopper, belonging to the technical field of biotechnology. The application comprises: using the CE1 gene to improve the resistance to brown planthopper. The present disclosure provides an application of a CE1 gene in improving resistance to brown planthopper, which comprises using the CE1 gene to improve the resistance to brown planthopper of rice, and the resistance to brown planthopper of the recipient plant can be significantly improved, and the effect is remarkable.

This invention discloses the application of the OsSMP3 gene in the control of rice brown planthopper, specifically disclosing that the OsSMP3 gene (LOC_Os11g05170) negatively regulates rice resistance to brown planthopper. By targeting this gene using CRISPR / Cas9 gene editing technology, a loss-of-function mutant was obtained. Compared to the wild type, the knockout mutant showed significantly enhanced resistance to brown planthopper, specifically manifested in increased rice plant survival rate. This invention provides new gene resources and targets for molecular breeding of rice resistant to brown planthopper.

The application discloses a gene for regulating brown planthopper resistance of rice METS2 and application thereof, and belongs to the technical field of genetic engineering. METS2 The CDS sequence of the gene is shown as SEQ ID NO. 3. Overexpression of the gene can significantly improve the resistance of rice to the brown planthopper, and provides important gene resources and technical support for rice pest resistance breeding. METS2 The gene overexpression type transgenic rice can be used as a new rice germplasm material, and is used for in-depth research on the brown planthopper resistance mechanism of rice, and for excavation of more genes for regulating the brown planthopper resistance, and has important application value for effectively improving the brown planthopper resistance of rice by means of genetic breeding and genetic engineering methods.

The present application provides a kind of brown planthopper RNA binding protein NlRBP gene inhibitor and application, the NlRBP gene inhibitor is the dsRNA capable of inhibiting brown planthopper NlRBP gene expression, the nucleotide sequence of the dsRNA is as shown in SEQ ID NO.3.The present application also provides the application of the above-mentioned brown planthopper RNA binding protein NlRBP gene inhibitor in the prevention and treatment of brown planthopper.The prevention and treatment of brown planthopper is to inhibit the survival rate of brown planthopper.The survival rate of brown planthopper is realized by inhibiting the expression of brown planthopper RNA binding protein NlRBP gene.The present application also provides the application of the above-mentioned dsRNA in preparation product.The present application provides a new prevention and treatment strategy and target for brown planthopper prevention and treatment, establishes the technology based on RNAi green control brown planthopper, provides foundation and reference for the precision, sustained pest control of brown planthopper, and has good application prospect.

The present application relates to the technical field of biological control of agricultural pests, and particularly relates to a method for improving pathogenicity of Metarhizium rileyi CQMa421 to Nilaparvata lugens by using carbon-hydrogen compounds on the body surface of the brown planthopper. The method for improving pathogenicity of Metarhizium rileyi to Nilaparvata lugens comprises mixing an effective dose of n-undecane liquid agent with the Metarhizium rileyi. The present application adopts the mixed use of n-undecane and Metarhizium rileyi, shortens the median germination time of Metarhizium rileyi, accelerates the initial process of infection, improves the pathogenicity to Nilaparvata lugens, and is conducive to rapid pest control. The n-undecane is a natural compound carried by insects, and the compound is safe to the environment and non-target organisms, and meets the development needs of green agriculture. The method only needs to simply mix the existing Metarhizium preparation with n-undecane before application, without the need to change the existing application equipment and process, and is easy to popularize and apply.

This invention provides a rice gene Bph54 for resistance to brown planthopper and its application. The rice provided by this invention... Bph54 Genes are of great significance for breeding new rice varieties resistant to brown planthoppers. This invention successfully cloned the rice Bph54 gene using map-based cloning, and performed functional annotation and identification. This gene exhibits complete dominance, and both homozygous and heterozygous genotypes confer equal resistance to brown planthoppers in rice. This characteristic is beneficial for the application of this gene in breeding. Based on this, the Bph54 gene can be introduced into brown planthopper-susceptible plant varieties through traditional breeding techniques such as hybridization and backcrossing, or through genetic engineering, to cultivate new varieties of plants resistant to brown planthoppers with stable genetic traits. This invention provides important germplasm resources for effectively reducing insect damage and achieving increased and stable crop yields.

The invention relates to the technical field of biology, in particular to a brown planthopper NlABCG1 gene, encoding protein thereof and application of dsRNA of the brown planthopper NlABCG1 gene in prevention and treatment of brown planthopper. The invention discloses an NlABCG1 gene. The function of the gene is inhibited, so that the survival rate of brown planthoppers is reduced; after the dsRNA of the NlABCG1 synthesized in vitro is used for silencing the NlABCG1 gene in a microinjection mode, the survival rate of the brown planthopper is remarkably reduced, and the shape and appearance of the brown planthopper are remarkably changed. The invention has a good application prospect, and is of great significance to prevention and treatment of brown planthopper.

This invention provides the application of the NlP21 protein and its encoding gene in regulating plant resistance to brown planthoppers, belonging to the field of genetic engineering technology. This invention provides the sequence of the NlP21 protein and its encoding gene in brown planthoppers. This gene is of great significance for regulating plant resistance to brown planthoppers and regulating the growth of brown planthoppers. Inhibiting the expression of the NlP21 gene in brown planthoppers can effectively reduce their survival ability, while overexpressing the NlP21 gene in plants can effectively improve plant resistance to brown planthoppers. The NlP21 protein and its encoding gene provided by this invention have excellent application prospects and significant application value for reducing pesticide use, maintaining ecological balance, and promoting sustainable development.

The present invention provides an indoxacarb-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is indoxacarb, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of chilo suppressalis. Experiments prove that the binary composition obtained by compounding flufenican amide and indoxacarb in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

The application belongs to the field of agricultural biotechnology, and particularly relates to a dsRNA of a brown planthopper gene and application of the dsRNA in preventing and treating the brown planthopper and related methods. NlUGT7 The dsRNA of the gene is prepared through in vitro transcription, and RNA interference treatment is performed on the brown planthopper through microinjection. NlUGT7 The results show that after the dsRNA is injected, the expression amount of the gene in the brown planthopper of biotype Y and biotype 1 is significantly reduced. NlUGT7 After the gene is silenced, the survival rate of the brown planthopper of biotype Y feeding on YHY15 rice containing a Bph15 resistance gene or the brown planthopper of biotype 1 feeding on TN1 rice not containing the resistance gene is significantly reduced. NlUGT7 The gene plays an important role in the survival process of the brown planthopper, and can be used as an effective target for preventing and treating the brown planthopper. NlUGT7 The gene and the dsRNA thereof provided by the application can be applied to research and development of biological pesticides for the brown planthopper and green prevention and control, and have good application prospects.

This invention belongs to the field of biological control technology for brown planthoppers, specifically relating to the application of the Tip60 gene and its encoded protein in the control of brown planthoppers. This invention is the first to discover that the Tip60 gene plays a crucial role in the control of brown planthoppers. Further experiments confirmed that reducing the expression level of the Tip60 gene significantly shortens the survival time of female brown planthoppers and reduces the number of offspring, indicating that inhibiting Tip60 gene expression can effectively control brown planthoppers, providing a theoretical basis for this strategy. Simultaneously, this invention uses RNAi technology to interfere with the expression of this gene during the adult stage of brown planthoppers, producing a significant inhibitory effect, thus providing a novel strategy and target for brown planthopper control, and demonstrating the application prospects of RNAi technology in silencing the Tip60 gene in brown planthopper control.

This invention provides rice OsPDRA3 Application of genes in improving insect resistance in rice. This invention clones genes from the rice genome. OsPDRA3 Gene (LOC_Os01g42380), analyzed using the SMART program, predicts that this protein possesses multiple transmembrane domains and ATPase domains (AAA+) associated with various cellular activities. Further investigation is needed. OsPDRA3 The effect on tolerance of rice brown planthopper was investigated by comparing wild-type and overexpression. OsPDRA3 The tolerance of transgenic plants to brown planthoppers was assessed, and the results showed that overexpression of [the planthopper species]... OsPDRA3 Compared to wild-type rice lines (0% survival rate), the survival rate of rice lines treated with brown planthoppers was significantly improved, reaching 75%–100%. This indicates that… OsPDRA3 Genes can significantly improve the insect resistance of rice and can be widely used in the fields of plant genetic engineering and plant disease resistance, with huge economic value and application prospects.

This invention relates to the field of agricultural technology, specifically to a mutant rice OsDSP1 gene and its applications. Compared to the wild-type rice OsDSP1 gene, the mutant OsDSP1 gene has a T base inserted after position 115 or a G base deleted at position 116. The mutant rice OsDSP1 gene provided by this invention can improve rice resistance to brown planthoppers, while also increasing grain length and thousand-grain weight, effectively increasing rice yield. This invention provides new gene resources and improvement targets for the breeding of insect-resistant rice varieties, and has significant application value for insect-resistant and yield-increasing plant breeding.

The present invention provides a chitin synthesis inhibitor compound-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is buprofezin, lufenuron, cyromazine, diafenthiuron, diflubenzuron, chlorbenzuron, hexaflumuron or chlorfluazuron, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially brown planthopper, beet armyworm or liriomyza sativae. The invention verifies that the binary composition of the inhibitor insecticide synthesized from the flufenutraniliprole and the chitin has a remarkable synergistic effect, effectively improves the control effect, reduces the dosage and expands the insecticidal spectrum. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I).

The invention provides indole C3-O functionalized derivatives as well as a preparation method and insecticidal and antibacterial application thereof, and relates to the technical field of pesticide development. The indole C3-O functionalized derivative provided by the invention has a structure of a general formula (1), is prepared through a free radical cyclization reaction catalyzed by N-heterocyclic carbene (NHC), does not need a transition metal catalyst, and efficiently constructs a C3-site oxygen functional group by taking o-nitroalkyne or cis-o-nitrostyrene and aldehyde as raw materials through multi-step series conversion in an inert atmosphere. The method has the advantages of being high in atom economy, good in functional group compatibility, easy and convenient to operate, environmentally friendly and the like. The obtained derivative shows remarkable activity in prevention and treatment of various agricultural diseases and insect pests such as rice sheath blight disease, aflatoxin and brown planthopper, can be prepared into various pesticide preparations, has the characteristics of broad spectrum, high efficiency and low toxicity, and is suitable for development of green pesticides.

The invention relates to the technical field of plant genetic engineering, and discloses a rice brown planthopper resistant gene Bph56, a molecular marker closely linked with the same and application of the rice brown planthopper resistant gene Bph56. The rice Bph56 gene provided by the invention is of great significance for cultivating a new variety of brown planthopper resistant rice. The rice Bph56 gene is successfully cloned through a map-based cloning method and is subjected to function annotation and identification, the gene is completely dominant, the homozygous and heterozygous genotypes endow rice with equal brown planthopper resistance, and the characteristic is beneficial to application of the gene in breeding. On the basis, the Bph56 gene can be introduced into a plant variety susceptible to brown planthopper through traditional breeding technologies such as hybridization, backcross and the like or genetic engineering means, and a novel brown planthopper-resistant plant variety with stable hereditary characters is obtained through cultivation. The invention provides important germplasm resources for effectively reducing insect pests and realizing yield increase and stable yield of crops.

The invention provides application of methyl selenocysteine in improving insect resistance of rice and a method, belongs to the technical field of agricultural plant protection, and particularly relates to application of methyl selenocysteine in treating rice in a root irrigation or stem and leaf spraying mode. During root irrigation, the concentration of the methyl selenocysteine is 2-10 [mu] M; when the stem leaves are sprayed, the concentration of the methyl selenocysteine is 20-100 [mu] M; the invention discloses that methyl selenocysteine has an excellent prevention and control effect on main piercing-sucking type pests, namely brown planthopper and sogatella furcifera, on rice for the first time, the survival rate of rice planthopper nymphs can be reduced to 10% or below, and the methyl selenocysteine has very high economic benefits.

The invention belongs to the technical field of pesticides, and particularly relates to a substituted heterocyclic compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, wherein Q represents X represents alkyl, halogenated alkyl and the like; y represents O or N-Y1; r4 and R5 each independently represent hydrogen, halogen, alkyl, or the like; r6 represents hydrogen, alkyl, haloalkyl and the like. The compound has excellent prevention and treatment effects on spodoptera litura, prodenia litura, armyworm, brown planthopper and the like.

The present invention provides a pyridaben-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is pyridaben, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention provides an application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of phyllotreta striolata. The invention verifies that the binary composition obtained by compounding the flufenoxanil and the pyridaben in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

The application discloses a breeding method of a salt-tolerant multi-resistance high-quality rice restorer line. A restorer line resistant to bacterial leaf blight, rice blast and brown planthopper is used as a female parent, a strong salt-tolerant germplasm is used as a male parent, and then F2 generation is obtained after self-crossing; 0‰, 3‰ and 5‰ salt gradient stress is carried out on the F2 generation under stable salt environment conditions, single plants with low glume degeneration rate and good plant leaf type are screened, and the single plants are crossed with Yuzhenxiang; the cross progeny is identified through multi-generation salt stress, the agronomic characters, resistance and rice quality of the cross progeny are screened under a non-salt environment, and salt-tolerant, multi-resistance, high-quality and fragrance characters are aggregated; the cross progeny is verified through natural salt stress in a coastal saline field, and a salt-tolerant multi-resistance high-quality rice restorer line with a relative yield loss rate of less than 40% in a 5‰ salt concentration, resistance to bacterial leaf blight, rice blast and brown planthopper, a grain length-width ratio greater than 4.0, a chalky grain rate lower than 7.0% and fragrance is obtained. The application integrates multi-gradient salt stress identification, intergenerational cross selection and multi-character aggregation, and creates the salt-tolerant multi-resistance high-quality rice restorer line.