Molecular marker method of rice variety brown planthopper resistance main gene Bph3

An anti-BPH and molecular marker technology, applied in the field of molecular genetics, can solve the problems of difficult-to-polymerize insect-resistant genes, etc., and achieve the effects of convenient identification, clear selection targets, and low reliability

Inactive Publication Date: 2009-04-29
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] Due to the complexity of insect resistance identification, it is often difficult to effectively aggregate different insect resistance genes using conventional breeding methods

Method used

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  • Molecular marker method of rice variety brown planthopper resistance main gene Bph3
  • Molecular marker method of rice variety brown planthopper resistance main gene Bph3
  • Molecular marker method of rice variety brown planthopper resistance main gene Bph3

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Embodiment Construction

[0032] Studies have shown that BPH-resistant gene resources mainly exist in indica and wild rice species in Sri Lanka and India (Ikeda and Vaughau, 1991). Athwal et al. (1971) reported that Mudgo, CO22 and MTU15 carried the same brown planthopper resistance gene Bph-1, and ASD7 carried a recessive insect resistance gene bph-2. Athwal and Pathak (1972) reported that MGL2 contains the insect resistance gene Bph-1, and Ptb18 contains bph-2. Martinez and Khush (1974) reported that IR747B2-6 contained Bph-1, and R1154-243 and IR4-93 contained bph-2. Lakshiminarayana and Khush (1977) reported that the Sri Lankan insect-resistant variety Rathu Heenati is controlled by a dominant gene Bph-3 that segregates independently from Bph-1; while the variety Babawee is controlled by a recessive gene bph-4 that segregates independently from bph-2 . Sidhu and Khush (1978) reported that rice varieties carrying Bph-3 or bph-4 were resistant to all BPH biotypes. The Thai rice varieties Col.5Thai...

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Abstract

The invention relates to a molecule tagging method for a brown paddy plant hopper resistant master gene Bph3 of rice cultivars, which belongs to the technical field of genetic thremmatology. The brown paddy plant hopper resistant master gene Bph3 of an insect resistant variety Rathu Heenati is obtained by genetic linkage analysis of the brown paddy plant hopper resistant level of gene types of various single plants of F2 and various genealogies of F 2:3 obtained after hybridization of the insect resistant rice variety Rathu Heenati (female) and an insect susceptible variety 02428(male). The gene is positioned between a molecular marker A4 and a molecular marker RM16533, and the selection efficiency of three Indel indexes namely RH784, RH786 and RH007 of the interval is approximately 97 percent. Detection is made whether the insect resistant variety Rathu Heenati and derivative varieties (systems) of the insect resistant variety Rathu Heenati contain the master gene through the molecular markeres of the brown paddy plant hopper resistant master gene; the brown paddy plant hopper resistance level of the master gene can be predicted; and the selection efficiency of brown paddy plant hopper resistant rice can be greatly improved.

Description

1. Technical field [0001] The invention provides a molecular marker method for rice varieties resistant to brown planthopper main gene Bph3, belongs to the field of molecular genetics, and is specially used for the selection of rice varieties resistant to brown planthoppers and the utilization of germplasm resources. 2. Background technology [0002] Rice is an important food crop in my country. The brown planthopper is a monophagous pest of rice, belonging to the family Plantagoidae of the order Homoptera. It is a typical piercing-sucking pest that sucks the phloem sap of rice stems through the stylet. In severe cases, the lower part of the rice plant will turn black, rotten and smelly, and paralyzed, which is called "lice burning", resulting in reduced or lost rice production. At present, the control of brown planthopper mainly depends on chemical pesticides, but this measure not only increases the production cost, but also pollutes the environment. Therefore, the most e...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 万建民刘裕强江玲吴寒何俊刘世家刘喜陈亮明
Owner NANJING AGRICULTURAL UNIVERSITY
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