The application discloses application of a trnP
gene in identifying C- and C+ strain
whitefly monogynous lines, primer pairs and an identification method, and belongs to the technical field of agricultural biological detection. In the C- strain
whitefly monogynous line, part of a trnP
gene is shown as SEQ ID NO. 3; in the C+ strain
whitefly monogynous line, part of the trnP
gene is shown as SEQ ID NO. 4. According to the different bases of the mitochondrial trnP gene in the C- and C+ strain whitefly mtDNA, the application develops primer pairs containing the different base sequences, and further develops a method for identifying whitefly monogynous lines based on PCR-RFLP, so that different strains of whiteflies can be identified quickly, accurately and intuitively, and the application lays a foundation for identification of heat-resistant whitefly monogynous lines,
population dynamic identification of whiteflies, biological research and research on an invasion mechanism of the whiteflies.