94 results about "Whitefly" patented technology

The present disclosure is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The disclosure discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The disclosure also discloses methods and compositions for targeted genome editing in the pest in order to achieve a reduction in pest infestation through disruption of protein activity. The disclosure is also directed to methods for making transgenic plants that express the double stranded RNA molecules and targeted genome editing constructs for use in protecting plants from pest infestation.

The present invention provides the use of 1-methoxyindole or its derivatives in repelling and / or killing pests, belonging to the field of pest control technology. The active ingredient provided by the present invention is 1-methoxyindole (1-MOI), which is found in Metarhizium robertsiense. When applied as a repellent, the concentration in a plane is 0.127 μg / cm 2 1-methoxyindole can repel a variety of ants, termites, and ticks, and the higher the 1-methoxyindole content, the stronger the repellent effect; when used as an insecticide, the concentration in the air is 0.14μg / cm 3 The 1-methoxyindole can kill a variety of ants, termites, ticks, thrips, mosquitoes, as well as whiteflies, brown planthoppers, spider mites and other pests, and the higher the concentration of 1-methoxyindole, the stronger the killing effect.

The invention relates to the technical field of pesticides, in particular to a pesticide composition, application thereof and a pesticide preparation. The pesticide composition comprises a first effective component and a second effective component, the first effective component is 1, 8-cineole, and the second effective component comprises at least one of flonicamid, cyantraniliprole, nitenpyram, flubendiamide, spirotetramat, pyriproxyfen, pymetrozine, dinotefuran and sulfoxaflor. The mass ratio of the first effective component to the second effective component is (50: 1)-(1: 100). Through the synergistic interaction of the first effective component and the second effective component, the prevention and treatment effect is remarkably improved, the prevention and treatment spectrum is expanded, and the field dosage can be greatly reduced; specifically, leaf back bemisia tabaci and trialeurodes vaporariorum can be well controlled, the control effect on bemisia tabaci, trialeurodes vaporariorum, thrips and the like is improved, and the application prospect is good.

The invention relates to a pesticide composition containing ecdysone compounds and application of the pesticide composition. The pesticide composition is composed of flufeniprole and methoxyfenozide or tebufenozide. The pesticide composition has an obvious synergistic effect, and the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The pesticide composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, tryporyza incertulas, rice leaf folders, plant hoppers, prodenia litura, beet armyworms, plutella xylostella, cabbage caterpillars, aphids, aleyrodids, thrips and flea beetles.

The present disclosure is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The disclosure discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The disclosure also discloses methods and compositions for targeted genome editing in the pest in order to achieve a reduction in pest infestation through disruption of protein activity. The disclosure is also directed to methods for making transgenic plants that express the double stranded RNA molecules and targeted genome editing constructs for use in protecting plants from pest infestation.

The present invention relates to tomato plants having improved insect resistance, more particularly whitefly resistance or mite resistance, wherein the plants comprise an ASAT3 gene encoding an acetyl-CoA-dependent acyltransferase and an AP2e gene encoding an APETALA2 ethylene response transcription factor. The invention also relates to a method for providing tomato plants with improved insect resistance, and the use of the ASAT3 gene in combination with the AP2e gene for providing insect resistant tomato plants.

The invention relates to a sulfite compound with chirality and isomerism and application thereof. The invention discloses a sulfite compound with chirality and isomerism as well as preparation, separation and application of a chiral structure of the sulfite compound, and belongs to the technical field of agriculture. The invention finds that the chiral and isomeric sulfite compound with a structure of a general formula (A) has excellent inhibitory activity on eggs, particularly has a very strong inhibitory effect on eggs of thrips, mites, lepidoptera moths, aleyrodids and harmonia axyridis, and also has bactericidal activity, and an absolute configuration of the compound is obtained through preparation and separation; the compound with the structure can be used for killing eggs and bacteria, has a very high pesticide research value, and has a wide application prospect in the field of agricultural pharmacology.

The invention discloses a botanical formula of a gerbera jamesonii thrip repellent and a preparation method thereof, and belongs to the technical field of botanical pesticides. The formula comprises the following components in parts by weight: 30-35 parts of tea saponin, 30-35 parts of cynanchum komarovii, 15-20 parts of rangooncreeper fruit, 5-8 parts of menthol, 3-5 parts of wormwood extract, polyoxyethylene sorbitan monooleate, ethylene glycol, citric acid or sodium hydroxide and the balance of deionized water, and the proportion of each core component in the optimal formula is accurately optimized. The preparation method comprises the steps of raw material pretreatment, ultrasonic-assisted extraction, slow fire decoction and filtration, concentration, compounding and emulsification, canning and storage and the like, and key process parameters are optimized to guarantee the extraction rate of effective components and product stability. Through the synergistic effect of multiple components, the thrip repellent rate reaches 95.3%-97.8%, the thrip repellent has a remarkable repellent effect on trialeurodes vaporariorum and red spiders, the lasting period is prolonged to 14-18 days, and the thrip repellent is suitable for various flowers and planting scenes.

This invention provides the application of dsRNA in the control of insecticide-resistant whiteflies, belonging to the field of pesticide technology, specifically a... BtUGPase The application of dsRNA of the gene in the control of bromocyanamide and fluoxazolamide-resistant whiteflies is discovered and confirmed in this invention. BtUGPase The gene is closely related to UGT-mediated resistance in whiteflies to bromocyanamide and fluoxazolamide. When whiteflies feed on... BtUGPase After dsRNA administration, resistance to bromocyanamide and fluoxazolamide was significantly reduced.

The application provides a G protein-coupled receptor (GPCR) inhibitor and application of a GPCR gene NPFF2 in pest control, especially in management of resistance of a new nicotine agent of a whitefly. The application is designed for a specific target GPCR, and development of a new insecticide or a related functional product, which can be applied to production and control of pests such as whiteflies, and especially provides application guidance for management of resistance of pests.

The application belongs to the technical field of medicine synthesis, and particularly relates to quinolinone derivatives, a synthesis method and application thereof. The quinolinone derivatives have a chemical structure as shown in formula (I). A series of novel quinolinone derivatives are first proposed in the application. In addition, the application further proposes a synthesis method of the quinolinone derivatives. The quinolinone derivatives are obtained through a series of reactions based on an indanone with different hydroxyl positions as a basic raw material. The product prepared by the synthesis method has high yield and is easy to separate, and the synthesis method is the optimal method for preparing the quinolinone derivatives. The application further provides a new use of the quinolinone derivatives. Biological tests prove that the quinolinone derivatives have good toxic killing activity on piercing-sucking insects such as aphids, armyworms, spider mites, rice planthoppers and whiteflies, and can be applied to plant pest control.

The application discloses application of a trnP gene in identifying C- and C+ strain whitefly monogynous lines, primer pairs and an identification method, and belongs to the technical field of agricultural biological detection. In the C- strain whitefly monogynous line, part of a trnP gene is shown as SEQ ID NO. 3; in the C+ strain whitefly monogynous line, part of the trnP gene is shown as SEQ ID NO. 4. According to the different bases of the mitochondrial trnP gene in the C- and C+ strain whitefly mtDNA, the application develops primer pairs containing the different base sequences, and further develops a method for identifying whitefly monogynous lines based on PCR-RFLP, so that different strains of whiteflies can be identified quickly, accurately and intuitively, and the application lays a foundation for identification of heat-resistant whitefly monogynous lines, population dynamic identification of whiteflies, biological research and research on an invasion mechanism of the whiteflies.

The present invention belongs to the technical field of pharmaceutical synthesis, and particularly relates to epiandrosterone pyrazole derivatives, their synthesis methods and applications. The epiandrosterone pyrazole derivatives have a chemical structure represented by the general formula (1), wherein R is an alkyl group or an aromatic ring-containing group. The alkyl group is an aliphatic chain or a cycloalkane, and the aromatic ring-containing group is a phenyl group or a substituted phenyl group. The synthesis method of the epiandrosterone pyrazole derivatives uses epiandrosterone as a basic raw material and obtains the epiandrosterone pyrazole derivatives of the present invention through a series of different reactions. The epiandrosterone pyrazole derivatives disclosed in the present invention are a series of brand-new compounds, which are proposed for the first time. The synthesis method has a high preparation yield, and the obtained product is easy to separate. It is confirmed by biological assays that the epiandrosterone pyrazole derivatives exhibit good insecticidal activity against pests such as aphids, oriental armyworms, diamondback moths and whiteflies, and can be applied to the control of plant pests.

The present disclosure relates to insecticidal combinations and compositions comprising two or more insecticide compounds. Particularly, the present disclosure provides insecticidal combinations for controlling insect pest selected from aphids, jassids, and whiteflies in crops such as cotton and okra. The disclosure also relates to corresponding methods and uses thereof.

The invention discloses a whole-cycle green comprehensive management method for waxberries, and belongs to the technical field of agricultural planting. According to the method, the compound microorganism fertilizer is applied underground, mineral oil is continuously sprayed on the ground in multiple growth stages to prevent and control aphids, whiteflies and scale insects, and multiple biostimulant combinations are accurately sprayed in the key phenological stage to comprehensively improve the quality of leaves and fruits. And the problems of demulsification, flocculation and phytotoxicity of mineral oil caused by water hardness in traditional mixing are effectively solved through an innovative barrel mixing process. According to the method, three technical means are systematically integrated to form a synergistic green management system, the problems that in traditional waxberry planting, chemical pesticide dependence is high, and the contradiction between pest control and quality improvement is prominent are effectively solved, and efficient, high-quality and sustainable development of waxberry production is achieved.

The present invention discloses the use of a BtRPSA gene in the control of whiteflies, as well as reagents and methods for controlling whiteflies, belonging to the technical field of insect pest control. The nucleic acid sequence of the whitefly BtRPSA gene is shown in SEQ ID NO. 1. A highly efficient silencing agent, dsBtRPSA, is prepared based on the BtRPSA gene. The dsBtRPSA is introduced into the whitefly through feeding, significantly reducing the heat tolerance of the whitefly, thereby achieving the purpose of control. This method is easy to operate, highly effective and specific, and has significant effects. It is also environmentally friendly and has many advantages, and has great application prospects in the control of whiteflies.

The invention discloses a kind of insecticidal composition containing methoxazole and D limonene and its application, the insecticidal composition includes active ingredient, the active ingredient is methoxazole and D limonene, and the mass ratio of the D limonene and the methoxazole is 1:50 to 50:1.Due to D limonene and methoxazole are compounded, so that the two can produce synergistic effect, then the insecticidal composition formed has good insecticidal effect, and has a larger prevention and control spectrum, especially for tomato whitefly and other piercing-sucking mouthparts pests have good prevention and control effect.In addition, D limonene is a plant-derived compound, and the combination of the two will not produce resistance while also delaying the development of other chemical resistance, and the insecticidal composition side effect is extremely low, will not affect crop safety and yield, etc., thus can solve the problem that tomato whitefly and other piercing-sucking mouthparts pests are difficult to prevent and control, achieve good insecticidal effect.

The invention discloses a cultivation method for promoting insect resistance and yield increase of tobacco through adjacent cropping of wormwood, and relates to the technical field of crop pest control. The method comprises the following steps: applying a base fertilizer in a row in to-be-cultivated soil, ploughing and ridging, transplanting cultivated wormwood seedlings and flue-cured tobacco seedlings in an adjacent cropping manner, after the wormwood seedlings are transplanted, watering rooting water for 1-2 times according to the soil moisture content, when the heights of the seedlings are 8-10cm, performing final singling according to the plant spacing of 25-35cm, and reserving one strong seedling in each hole; after the tobacco seedlings survive, timely uncovering the film, intertilling, weeding and ridging, timely topping and stubble wiping in the later period, reserving 20-24 effective leaves on tobacco plants, starting to harvest the tobacco leaves in the middle ten days of July, harvesting once every 10 days, and baking for 10 days; after the tobacco leaves are harvested, the tobacco stalks are removed, and the wormwood is harvested in early September. The method effectively reduces the harm rate of pests with chewing mouthparts to tobacco plants, increases the population number of natural enemies of myzus persicae to control the number of pests such as myzus persicae and bemisia tabaci, improves the yield, quality and safety of tobacco leaves, is environment-friendly, and is beneficial to continuously improving the ecological system of a tobacco field.

The invention discloses an insecticidal preparation based on rosemary-mint mixed oil and a preparation method and application thereof, and belongs to the crossing field of green agriculture and biotechnology. The mixed essential oil is efficiently extracted through an ultrasonic-subcritical coupling extraction process, the rosemary oil extraction rate can reach 12.1%-12.4%, the menthol retention rate reaches 95.2%-97.1%, and the solvent residue is lower than 0.01 mg / kg; a full-plant-source ternary compound system (mixed essential oil, sophora alopecuroide extract and citrus peel oil) is designed, the synergistic coefficients of 50 formulas are all larger than or equal to 1.25, and six types of pests including thrips, red spiders, aphids, whiteflies, plutella xylostella and leafhoppers can be covered; a special application technology for eight types of crops (including tea leaves and traditional Chinese medicinal materials) is established, the quality of the crops reaches the standard after pesticide application, for example, the water extract of the tea leaves is increased by 1.2%, and the astragaloside content meets the standard of Chinese Pharmacopoeia 2020 edition; the storage stability is optimized, the control effect retention rate can reach 94.7% after 6 months of normal-temperature storage, and the requirement of 2-year industrial shelf life can be met; finally, the average reduction rate of the chemical pesticide is 90.3%, and the preparation cost is less than or equal to 80 yuan / kg. The composition can replace chemical pesticides and is suitable for green prevention and control of insect pests of fruits and vegetables, fields, commercial crops and traditional Chinese medicinal materials.

This invention discloses an indoor breeding method for ladybugs, belonging to the field of biological control. This invention uses beet armyworms to partially replace whiteflies, the natural food source for ladybugs at different developmental stages, thereby reducing the amount of whiteflies required. Since the artificial feeding technology for beet armyworms is currently very mature, beet armyworm eggs can be obtained stably and efficiently. Therefore, this invention, by using beet armyworm eggs to partially replace whiteflies, the natural food source for ladybugs, simplifies the indoor breeding process of ladybugs, reduces breeding costs, and has good application prospects.

The invention relates to a compound with a cyclopropane carboxylic acid pyrazole ester structure and application thereof, and belongs to the technical field of pesticides. The structure of the compound is shown in the specification. The invention further provides application of the compound shown in the formula I in prevention and control of agricultural pests. The compound represented by the formula I provided by the invention shows an excellent effect on various pests, especially on prevention or control of pests such as aleyrodidae, aphididae, tetranychidae and glophidae, and the compound has low toxicity.

This invention discloses primer pairs and methods for identifying monosexual whitefly strains based on the mtCOXI gene, belonging to the field of agricultural biodetection technology. The primer pair sequences for identifying monosexual whitefly strains based on the mtCOXI gene are shown in SEQ ID NO.1 and SEQ ID NO.2. Based on the differential bases in the mitochondrial mtCOXI gene of C- and C+ strains of whiteflies, this invention developed primer pairs containing these differential base sequences, and subsequently developed a PCR-RFLP-based method for identifying monosexual whitefly strains. This method can rapidly, accurately, and intuitively identify different strains of whiteflies, laying the foundation for the identification of heat-resistant monosexual whitefly strains, the identification of whitefly population dynamics, and research on the biology and invasion mechanisms of whiteflies.

The utility model provides a device for collecting small insects in the open air, including air suction pump and the air suction hose of connecting air suction pump, still including with the detachable connection of air suction hose intermediate connecting pipe, and with the detachable connection of transparent insect collecting tube of intermediate connecting pipe, the opening is provided in the tail end of insect collecting tube, is fixedly provided with gauze near intermediate connecting pipe one end. The utility model is used for the suction collection of small volume insects, such as thrips, whitefly and the like small volume insects, provides suction force through air suction pump, and air suction hose can realize the operation of various angles, and intermediate connecting pipe is used for connecting air suction hose and insect collecting tube, in order to facilitate production insect collecting tube, avoids the escape of insects, and gauze is fixedly set in the end part, prevents the escape of insects that are sucked into insect collecting tube, and the number of collected insects and the monitoring of insect state are more convenient to observe with the transparent pipe of insect collecting tube, and the tail end opening is plugged after single collection is completed.

The present invention relates to tomato plants having improved insect resistance, more specifically whitefly or mite resistance, comprising the ASAT3 gene encoding an acetyl-CoA-dependent acyltransferase enzyme and the AP2e gene encoding the APETALA2 ethylene-responsive transcription factor. The present invention further relates to methods for preparing tomato plants with improved insect resistance and to the use of the ASAT3 gene in combination with the AP2e gene to provide insect-resistant tomato plants.

The invention discloses a gene related to cadmium sensitivity of bemisia tabaci and application of the gene, and relates to genetic engineering and biological control technologies, the gene is BtHSP1 and / or BtCTSB2, the nucleotide sequence of the BtHSP1 gene is as shown in SEQ ID No.1, and the nucleotide sequence of the BtCTSB2 gene is as shown in SEQ ID No.2. The invention also provides dsRNA (double-stranded ribonucleic acid) for interfering the expression of the BtHSP1 gene and the BtCTSB2 gene. The death rate and the cell damage degree of the bemisia tabaci under cadmium stress are improved by inhibiting the expression of the bemisia tabaci BtHSP1 gene or promoting the expression of the bemisia tabaci BtCTSB2 gene. The BtHSP1 and BtCTSB2 genes have the effect of regulating and controlling the cadmium stress resistance of the bemisia tabaci and can be used as targets for preventing and controlling the bemisia tabaci under the cadmium stress, and a new idea is provided for preventing and controlling the bemisia tabaci in a cadmium-polluted area.

The present invention discloses a tomato whitefly-resistant gene S1PR3 and its application, belonging to the field of plant genetic engineering technology. The nucleotide sequence of the gene is shown in SEQ ID NO.1; the CDS of the tomato whitefly-resistant gene S1PR3, whose CDS sequence is shown in SEQ ID NO.2; and the amino acid sequence of the expressed protein of the tomato whitefly-resistant gene S1PR3, whose amino acid sequence is shown in SEQ ID NO.3. The present invention demonstrates through real-time fluorescence quantitative technology that the expression level of the S1PR3 gene changes significantly after being attacked by whiteflies, indicating its role in tomato whitefly resistance. The present invention also constructs tomato plants overexpressing the tomato whitefly-resistant gene S1PR3 and detects the resistance phenotype of the S1PR3 gene when the plants are attacked by whiteflies, demonstrating its resistance to whiteflies. The present invention has important application value in the breeding of insect-resistant crop varieties.

The embodiment of the invention discloses a 4-(aryl / alkoxy)-2, 6-dialkyl aniline derivative, a preparation method and application of the 4-(aryl / alkoxy)-2, 6-dialkyl aniline derivative in trialeurodes vaporariorum resistance, and belongs to the technical field of pesticides. The 4-(aryl / alkoxy)-2, 6-dialkyl aniline derivative has a structure as shown in a general formula (I), R1 and R2 are isopropyl, cyclopropyl or n-propyl, R3 is pyridyl, pyrazinyl, phenyl, halogen or trifluoromethyl substituted phenyl and C3-C5 alkyl, and R4 is pyridyl, pyrazinyl, phenyl and C1-C5 alkyl. The 4-(aryl / alkoxy)-2, 6-dialkyl aniline derivative provided by the invention has better trialeurodes vaporariorum resistance activity. The synthesis method provided by the invention is simple, the raw materials are cheap and easy to obtain, and a novel aleyrodid-resistant pesticide is expected to be developed. (I)

The invention discloses an insecticidal composition of a compound medicament for preventing and controlling bemisia tabaci, pea aphid and corn aphid, and belongs to the technical field of pesticides. The invention relates to a plant source compound huperzine A, apigenin and psoralen B, which are respectively compounded with five different emulsifiers to form the plant source compound huperzine A, apigenin and psoralen B, and the emulsifiers mainly comprise any one of sulfosuccinic acid-1, 4-dioctyl ester, fatty alcohol-polyoxyethylene ether, NP-10, Tween 20 and Tween 500. Huperzine A, apigenin, psoralen B and emulsifiers with different concentrations are compounded to obtain the insecticide composition, and the synergistic effect is achieved through the interaction of the emulsifiers and the compound. The compound pesticide is used for preventing and controlling horticultural crop pests such as bemisia tabaci and aphids, the usage amount of the pesticide can be reduced, pesticide residues on crops can be reduced, the preparation process is simple, raw materials are input, all raw materials are converted into finished products, no pollution is caused to the environment, and wide market prospects are achieved.

The invention relates to the field of plant protection attractants, in particular to a ladybird attractant as well as a preparation method and application thereof, and the ladybird attractant comprises a compound of 100mg / mL of 3, 7-dimethyldecane and 100mg / mL of 2-octyldecane-1-alcohol in a volume ratio of 1: 1. The attractant provided by the invention can obviously attract the heteroladybird, the proportion of attracted female insects to attracted male insects is close to 1: 1, and the attractant is helpful to attract amphiprotic aphids, aleyrodids, lepidoptera larvae and other agricultural insect enrichment areas in a balanced manner, so that the mating search time is shortened, and the mating quality and the egg laying amount are improved. The main components of the paradise axyridis attractant are all derived from paradise axyridis volatile matters, so that the paradise axyridis attractant is environment-friendly and safe to non-target organisms, has a natural pest control effect on aphids, whiteflies, lepidoptera larvae and other agricultural pests on plants, and has the effects of protecting crops and reducing the invasion of the agricultural pests.

The present invention belongs to the technical field of crop pest control, and relates to a natural enemy attractant of Encarsia formosa for the green prevention and control of whitefly pests and its use. The present invention provides a natural enemy attractant of Encarsia formosa, which is composed of a main component of the attractant and a solvent; the main component of the attractant is composed of pest-induced plant volatiles, healthy plant volatiles, nectar flower fragrance volatiles, and whitefly-related volatiles of pests. The present invention also simultaneously provides the use of the above-mentioned natural enemy attractant of Encarsia formosa: the attractant can significantly attract Encarsia formosa while repelling whitefly pests. The attractant of the present invention has good selectivity and can repel pests.