The invention relates to a sterilizing composition containing active ester, which comprises effective components such as active ester (A) and a strobilurin bactericide (B), wherein the strobilurin bactericide is chosen from azoxystrobin, kresoxim-methyl, pyraclostrobine, metominostrobin, dimoxystrobin, trifloxystrobin, picoxystrobin, fluoxastrobin, enestroburin, 2-[2-(2,5-phenoxy-dimethyl) phenyl-methyl-3- methoxy acrylate (ZJ0712) or 2-imino-methyl-N-methyl-2-[[[[[[4-(2,6-dimethyl)phenyl]-3-butylene-2-yl]imino]epoxide]phenyl]amide (SYP-1620), the weight ratio of the A and B is 100:1-1:100, and the content of the active component in the composition is 5-80 in percentage by weight. The composition can be prepared into suspending agents, wetting powder, water dispersible granules, and the like. The sterilizing composition can be applied to farmlands, orchards and the like and is used for controlling various plantdiseases of grains, vegetables, fruit trees, flowers, and the like, in particular powdery mildew and downy mildew.