The invention relates to the field of pesticide preparations, and discloses a sterilization composition and application thereof. The sterilization composition disclosed by the invention comprises an alternaria tenuissima activator protein and an active ingredient A, wherein the weight ratio of the alternaria tenuissima activator protein to the active ingredient A is (1:50)-(30:1), the active ingredient A is selected from amide fungicides, strobilurin fungicides, pyrrole fungicides, triazole fungicides, pyrimidine fungicides, pyridine fungicides or carbamate fungicides, and one or more than two fungicides is selected from each type of fungicides. According to the sterilization composition, the amide fungicides, the strobilurin fungicides, the pyrrole fungicides, the triazole fungicides, the pyrimidine fungicides, the pyridine fungicides or the carbamate fungicides and the alternaria tenuissima activator protein are compounded, and have complementary advantages, thus the resistance of vegetables on diseases can be improved through once application of the sterilization composition, the yield of vegetable crops can be increased, and the amount and frequency of applying the pesticide are reduced.