42 results about "Mitochondrial cytochrome" patented technology

The invention belongs to the technical field of food quality and safety detection and particularly relates to a method for rapidly detecting pork, beef, mutton and chicken components in food at the same time through animal genomic DNA (deoxyribonucleic acid) extraction, primer design and UP-M-PCR (universal primers-multiplex-PCR). The four meat components can be distinguished rapidly at the same time according to differential sites of mitochondrial cytochrome b of the animal by using the forward primer sharing and reverse primer specificity strategy and an M-PCR system including five primers. The result shows that the method has the advantages that the detection limit can be up to the pictogram level, the components of the reaction system are free from cross interference and the meat products on market can be detected, verified and distinguished accurately by sampling 80 meat products randomly according to the specificity of the four target meat components. In a word, the invention provides a specific, sensitive and practical multiplex PCR method for rapidly screening four common meat components in food at the same time.

The invention belongs to the field of agriculture, and relates to a bactericidal activity compound composition, in particular to a composition containing picarbutrazox and a mitochondrion-cytochrome-enzyme-inhibitor bactericide and a preparation thereof and applications of the composition and the preparation. The bactericidal composition comprises a bactericidal activity compound I and a bactericidal activity compound II, the bactericidal activity compound I is the picarbutrazox, and the bactericidal activity compound II is the mitochondrion-cytochrome-enzyme-inhibitor bactericide. The bactericide composition has the advantages of being wide in control spectrum and free of cross resistance, the using quantity and the using number of the picarbutrazox and the mitochondrion-cytochrome-enzyme-inhibitor bactericide can be obviously decreased, and the resistance is delayed. The invention also provides the preparation of the bactericidal composition, a preparation method suitable for the composition is designed, the effect of a medicament can be developed to a maximum degree, and the using quantity of the medicament is decreased. The invention also provides the applications of the bactericidal composition and the preparation, so that medicine is guided to be correctly used, and misusing of the medicament is reduced.

The invention discloses a method for detecting a duck-derived component in food through a fluorescent real-time PCR method. The invention relates to the animal species and animal-derived component identification technical field, particularly relates to the method for the duck-derived component in the food, and especially relates to the method for detecting the duck-derived component in the food through the fluorescent real-time PCR method. The invention aims to identify whether the food contains the duck-derived component. According to a duck mitochondrial cytochrome b (cytb) gene, a duck-derived specific primer and a probe system are designed, the duck-derived component in the food is detected, the operation is simple and fast, results are accurate and reliable, an identification system of a food meat type source is further improved, and consumer rights and interests are safeguarded.

The invention belongs to the technical field of molecular biology, and relates to a method for screening traditional Chinese medicine monomers, specifically relating to a method for screening monomer ingredients which have protective effect for oxidative damages to liver cells from Chinese traditional medicines by using mitochondrion index technique. In the invention, mitochondrial function detection is used as the core technology and methods of cell proliferation detection, mitochondrial reactive oxygen species generation detection, mitochondrial membrane potential detection, energy substance ATP in mitochondria detection and mitochondrial cytochrome C detection are adopted. Mitochondrial function changes are detected after H7702 cells, which are subject to oxidative damages induced by H2O2, are treated by the traditional Chinese medicine monomers of SalB, protocatechualdehyde, sodium danshensu, cordyceps polysaccharose, cordycepin and cordycepic acid, and the traditional Chinese medicine monomers, which have protective effect for oxidative damages to liver cells, are screened. The invention further provides applications of the traditional Chinese medicine monomers screened in preparing drugs for preventing and treating liver diseases such as fatty liver, viral hepatic diseases and liver fibrosis.

The invention discloses a method for identifying dried Pheretima aspergillum based on a PCR-RFLP technology. Universal primers of a COI gene are used to carry out PCR amplification to obtain mitochondrial cytochrome oxidase (COI) gene fragments of all kinds of earthworms, the sequences of the fragments are sequenced and compared, the complete sequences of the COI fragments of the different kinds of earthworms undergo restriction enzyme map analysis by DNAMAN software to finally screen a restriction enzyme RsaI, the restriction enzyme RsaI is used to carry out enzyme digestion on the above obtained PCR product, the enzyme digestion result of the PCR product is detected by 2% agarose gel, and the enzyme digestion maps are used to identify the dried Pheretima aspergillum and other varieties.The method is simple and reliable to operate, and provides a new technical way for the identification of dried Pheretima aspergillum products.

The invention provides a method for rapid identification of adelphocoris suturalis populations in middle area and edge area by virtue of a mitochondria molecular marker, and in particular identification of populations in Hebei and Hubei and populations in Gansu and Guizhou. According to the method disclosed by the invention, specific primers are respectively designed in accordance with three genes, namely mitochondria cytochrome oxidase I (COI), cytochrome b (Cytb) and mitochondria NADH dehydrogenase 5 (ND5), and PCR amplification products are subject to serial comparison, so that identification of the adelphocoris suturalis populations in middle area and edge area of China is achieved. The method is simple and reliable, strong in operability, good in stability and strong in repeatability.

The invention provides a method for rapidly identifying Lygus pratensis populations, especially Xinjiang populations, Gansu populations and Ningxia populations, by mitochondrial molecular markers. According to the method, specific primers are designed according to four gene segments including mitochondrial cytochrome oxidase II (COII), cytochrome b (Cytb), mitochondrial NADH dehydrogenase subunit 5(ND5) and 16S rDNA of Lygus pratensis respectively, and PCR (polymerase chain reaction) amplification products are subjected to series-connection comparison, so that identification for the Lygus pratensis populations in China is realized. The method is simple and reliable and has high operability, good stability and high repeatability.

The invention relates to qualitative detection methods of ingredients of animal origin in food, forage, fur, and products of food, forage and fur, and more specifically relates to primers and probes used for detecting ingredients of racoon dog origin, and a detection method of the ingredients of racoon dog origin. According to the detection method, gene sequences of mitochondrion cytochrome oxidase b are selected, a set of specific primers and probes is designed, and the specific primers and probes are used for real-time fluorescent PCR, so that simple, convenient and fast detection of the ingredients of racoon dog origin in food, forage, fur, and the products of food, forage and fur is realized.

The invention relates to a method for identifying pacific mackerel and slimy mackerel on basis of mitochondrial cytochrome b sequence, which comprises the following steps: respectively extracting genome DNA (deoxyribonucleic acid) in muscle tissues of pacific mackerel and slimy mackerel; carrying out PCR (Polymerase Chain Reaction) amplification reaction; sequencing the PCR product, and correcting; and constructing a Cytb base matrix. The method provided by the invention can be used for identifying the pacific mackerel and slimy mackerel in a simple, quick, accurate and efficient way.

The invention relates to a method for identifying Gaoyou duck varieties by utilizing molecular bioinformatics, belonging to the technical filed of biological variety identification. In the invention, the sequences of gene b of cytochrome of mitochondrial DNA of a Gaoyou duck and the duck variety to be tested are accurately obtained on the basis of gene cloning and DNA sequencing technologies, a plurality of bioinformatics software is utilized to accurately figure out the haplotype, variation locus, haplotype diversity, average nucleotide difference, nucleotide ramification degree, pure genetic distance, Kimura dual-parameter genetic distance among varieties, variety colony cluster result images and haplotype phylogenetic trees of each variety, and the genetic information difference among the varieties is utilized to identify the true and false of the Gaoyou duck varieties through software precise calculation and analysis results.

The invention relates to a method for detecting endotoxin poisoning of livestock by mutation of host liver mitochondrial gene, which saves time and effort and has credible results. According to the method, host liver mitochondrial DNA (deoxyribonucleic acid) is extracted, a gene specific primer of mitochondria cytochrome C oxidase (COI) is designed, and a liver COI gene segment is analyzed and sequenced by a PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) technology; and if a sample PCR stripe of a livestock poisoned by endotoxin swims abnormally, and the sequenced COI gene segment is subjected to T-G replacement point mutation simultaneously at 5322 and 5331, the host body is poisoned by the endotoxin and has toxic effect. The method has good repeatability, specificity and sensibility, and has good application value.

The invention belongs to the technical field of food quality and safety detection, and particularly relates to a PCR detection method for identifying duck meat mixed in mutton. A universal upstream primer F and two specific downstream primers R sheep and R duck are designed with mitochondrial cytochrome b gene of mutton and duck meat as characteristic target gene, a primer mixture is formed according to a certain concentration ratio, specific amplification fragments with different lengths can be obtained through one-time PCR, and analysis is conducted according to the size of molecular weight of strips obtained through electrophoresis detection. If specific strips appear on a sample to be detected at the positions of 367bp and 480bp, it proves that duck meat is mixed in the mutton sample; if specific strips appear on the sample to be detected at the position of 367bp and do not appear at the position of 480bp, it proves that no duck meat is mixed in the mutton sample. The method is accurate, reliable, simple and quick and can be used for screening species of meat in food and detecting and supervising meat adulteration.

The invention relates to a real-time fluorescence PCR detection method of Thelenota ananas; and moreover, primers and a probe with species specificity are designed on basis of a highly species-specific Thelenota ananas mitochondrial cytochrome oxidase subunit I sequence (GenBank: FJ589205.1) so as to realize species identification on the Thelenota ananas by adopting a Real-Time PCR method. Owe torelatively high specificity and sensitivity of the primers and the probe, the real-time fluorescence PCR detection method of the Thelenota ananas can be used for identifying authenticity of deep processed sea cucumber products.

The invention relates to a method for detecting the polymorphism of 12 loci of human mitochondrial cytochrome b genes simultaneously, and provides a method for detecting the polymorphism of 12 loci of mitochondrial cytochrome b genes (Cytochrome b, Cytb) simultaneously in the same system, which realizes the detection of the polymorphism of the 12 loci of the cytochrome cytb genes simultaneously in the same reaction system by combining the technology of polymerase chain reaction (PCR) with the technology of single basic group extension and fluorescence microarray hybridization. The fluorescence intensity and fluorescence type which are generated by single basic group extension only correspond to a genotype, and a polymorphic locus has only three kinds of characteristic fluorescence at most, and thus the genotypes of samples corresponding to all the same peak types can be obtained only by one-time DNA sequencing verification. Compared with a detection method by direct sequencing, the method of the invention has short time, namely experiments are finished within one day, and has high flux, namely the 12 polymorphic loci are detected directly in the same reaction; in addition, the method has low cost and visual result judgment.

The invention relates to a difunctional nanoprobe for detecting mitochondrial cytochrome C as well as a preparation method thereof. The preparation method comprises the following steps: taking a ligand of the mitochondrial cytochrome C; labeling fluorescein and aldehyde group on two ends of the ligand respectively; then adding magnetosome into the ligand again, and incubating overnight; then removing substances which do not react; then adding 1-(3-dimethyl amino propyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and methoxy-carboxylation-polyethylene glycol, and incubating overnight; and then removing substances which do not react, thereby obtaining the difunctional nanoprobe for detecting the mitochondrial cytochrome C. The difunctional nanoprobe and the preparation method thereof, which are provided by the invention, have good biocompatibility; the prepared probe for detecting mitochondrial cytochrome C has good dispersibility.

The present invention provides a method for controlling a soybean rust fungus having an amino acid substitution of F129L on mitochondrial cytochrome b protein, by applying a compound represented by formula (I) [wherein Q represents a group represented by the following Q1, Q2, Q3, Q4 or Q5 (in the formulae, ● represents a binding site to benzene ring); X represents an oxygen atom or NH; L represents CH2, an oxygen atom or NCH3; E represents a C6-C10 aryl group, etc.; R1 represents a C1-C3 chain hydrocarbon group or a cyclopropyl group, etc.; R2 represents a C1-C3 chain hydrocarbon group or a cyclopropyl group, etc.; R3 represents a C1-C3 alkoxy group or a C1-C3 chain hydrocarbon group, etc.; and n is 0, 1, 2, or 3] or its N oxide or an agriculturally acceptable salt thereof.

The invention discloses a molecular identification method of a mouse species, which comprises the following steps of: extracting genome DNA of the mouse species to be detected; respectively amplifyingmitochondrial cytochrome C oxidase subunit I gene and mitochondrial cytochrome b gene by using specific primers, and then sequencing; carrying out Blast nucleic acid sequence homology search on the NCBI website, wherein the sequence with the highest homology in the comparison result is the mouse species with the closest genetic relationship. According to the molecular identification method of themouse species, a PCR primer and a reaction program have strong universality, the method is simple and easy to operate, the mouse identification can be quickly and accurately performed, and the defectof morphological identification is overcome; meanwhile, the accuracy of the identification result is improved by mutually verifying two gene sequences of CO I and cyt b. In addition to taxonomicallyidentifying the mouse species, the molecular identification method of the invention is important for the study of biological genetic diversity and phylogenetic evolution by analyzing the sequence characteristics of the CO I and cyt b genes between species.

The invention provides application of rhIL-1Ra in preparing medicaments for treating acute liver failure. Compared with a control group, the rhIL-1Ra can obviously inhibit the activity of alanine aminotransferase and aspartate aminotransferase in serum, the death of liver cells is reduced, and the proliferation of the liver cells is promoted, so that the rhIL-1Ra has obvious therapeutic effects ofprotecting liver injury and promoting liver regeneration. In addition, the rhIL-1Ra inhibits apoptosis mediated by caspase-3, caspase-8 and caspase-9 activation in liver tissue by decreasing mitochondrial cytochrome c release. In summary, the rhIL-1Ra provides a strategy for the preparation of candidate drugs capable of reducing hepatocyte apoptosis for the treatment of APAP-induced acute liver failure and liver injury.

The invention belongs to a qualitative detection technology of animal-derived ingredients in feather down products and specifically relates to a primer / probe group for detecting a goose down component in the feather down product. A group of specific primer and probe is designed by selecting a mitochondrial cytochrome B (CYTB) gene sequence of goose down. By using the primer and the probe and adopting a real-time fluorescence PCR (polymerase chain reaction) technology, the goose down component in the feather down product can be fast, sensitively and specifically detected. The primer and the probe can be provided together with other reagents in the form of a kit and be used for nucleic acid amplification reaction. The method is simple and convenient to operate and good in repeatability.

The invention provides a fluorogenic quantitative PCR specific primer, a probe and a kit of the primer and relates to the technical field of food check and molecular biological detection. The primer, the probe combination, real-time fluorescence PCR and the detection kit are utilized and combined, and therefore the camel source ingredients contained in meat products can be identified fast. The real-time fluorescence quantitative PCR technology and the fluorescence probe nucleic acid DNA molecular marker technology are applied, the camel source specific primer and the specific probe are designed according to the mitochondrion cytochrome b (Cytb) gene height keeping area, and the primer has the advantages of being high in sensitivity, good in accuracy, fast, small in degradation degree (mtDNA is kept completely in the processing process), stable and easy to operate.

The present invention relates to a sterilization activity compound composition, particularly to a zinc 2-mercaptobenzothiazole and mitochondrial cytochrome enzyme inhibitor (QoIs) bactericide composition, a preparation and applications thereof. The sterilization composition contains a sterilization activity compound I and a sterilization activity compound II, wherein the sterilization activity compound I is zinc 2-mercaptobenzothiazole, and the sterilization activity compound II is at least one selected from mitochondrial cytochrome bactericides such as azoxystrobin, kresoxim-methyl, trifloxystrobin, pyraclostrobin, enestroburin, pyraoxystrobin, fenaminstrobin and cyazofamid. According to the present invention, the sterilization activity compound composition has effects of effectively reduction of drug resistance generation of diseases, and substantial reduction of resistance risk caused by the single use of the QoIs bactericide and the zinc 2-mercaptobenzothiazole; and the prevention and control effects of the QoIs bactericide and the zinc 2-mercaptobenzothiazole on fungal diseases, bacterial diseases and other diseases can be completely provided, and the good protection performance can be provided.

The invention relates to a specific primer for amplifying the gene sequence of mitochondrial cytochrome c oxidase I (COI) of aleyrodidae insects. The sequence is represented by the SEQ ID No.1 and SEQ ID No.2. The provided amplification primer can rapidly and effectively amplify COI genes of multiple species of aleyrodidae insects, and thus can provide reliable references for the researches on growth relationship of aleyrodidae system, and population genetic differentials and genetic structure of specific insects.

A method is provided which, by applying a compound represented by formula (1) or an N-oxide or agriculturally acceptable salt thereof, controls soybean rust fungus having an amino acid substitution ofF129L in the mitochondrial cytochrome b protein (in the formula, Q represents a group represented by Q1, Q2, Q3, Q4 or Q5 (. represents the binding site to the benzene group), X represents an oxygenatom or NH; L represents CH2, anoxygen atom or NCH3;E represents a C6-C10 aryl group, etc.; R1 represents a C1-C3 chain hydrocarbon groupe, a cyclopropyl group, etc.; R2 represents a C1-C3 chain hydrocarbon group, a cyclopropyl group, etc.; R3 represents a C1-C3 alkoxy group or a C1-C3 chain hydrocarbon group; and n represents 0, 1, 2 or 3).

The invention relates to a preparation method of polylactic-co-glycolic acid (PLGA) nano-particles encapsulated with nano insulin. In L6 skeletal muscle cells, the product disclosed by the invention has good effects in the field of treatment of glucose absorption function damage caused by arsenic, insulin resistance and mitochondrial dysfunction, and different biomarkers, for example, pyruvate kinase, glucokinase, ATP / ADP ratio, a mitochondrial membrane, cytoplasm release of mitochondria cytochrome, cell membrane potential and calcium ion level indicate that the function of mitochondria is improved. The product disclosed by the invention has a great application prospect in treatment of diabetes mellitus caused by arsenic.

The invention belongs to the technical field of the detection of mouse original components, and particularly relates to a method for detecting the mouse original components by a specific primer. The craft process of method comprises the following five steps: specific primer design synthesis, DNA (Deoxyribonucleic Acid) extraction, reaction system establishment, PCR (Polymerase Chain Reaction) amplification and the analysis result evaluation of the mouse original components. The specific primer is designed and synthetized according to the mitochondrial cytochrome b gene sequence of a mouse in Genbank, wherein the base sequence of the forward primer F of the specific primer is 5'-GCCTTATAATTAATTGGAGGTA-3', and the base sequence of the downstream primer F of the specific primer is 5'-AGCTATATTATGTGCTTGAT-3'; on the basis of the mitochondrial cytochrome Cyt b gene sequence of the mouse, a real-time quantitative PCR method is used for detecting the mouse original components so as to bring good specificity and repeatability. When the real-time fluorescence quantitative PCR method carried out by the primer is used for detecting the mouse original components, operation is simple, short time is consumed, and specificity is high. Secondary pollution caused by product amplification when an amplification product is taken out to carry out electrophoresis identification in a traditional detection method is avoided, and a new approach is provided for the detection of the mouse original components.

The present invention relates to the use of strobilurine type compounds of formula I and the N-oxides and the salts thereof for combating phytopathogenic fungi containing a mutation in the mitochondrial cytochrome b gene conferring resistance to Qo inhibitors, and to methods for combating such fungi. The invention also relates to novel compounds, processes for preparing these compounds, to compositions comprising at least one such compound, to plant health applications, and to seeds coated with at least one such compound.

The invention discloses a photobiological regulation therapeutic instrument for treating age-related macular degeneration and a use method. According to the photobiological regulation therapeutic instrument, by setting near-infrared light with a wavelength of 600-800 nm relative to a brightness of left and right eye sight glasses, two eyes of a patient are treated under a light-emitting viewpoint for 4 times every day and 40 seconds every time, so that activity of mitochondrial cytochrome c oxidase is improved, energy supply and cell metabolism are increased, tissue metabolism repair is enhanced, cone cell death of age-related macular degeneration patients is slowed down, and greater possibility is provided for vision recovery of the patients. The photobiological regulation therapeutic instrument is high in safety, low in price of a low-dose red light therapeutic instrument, low in treatment cost of patients and beneficial to popularization.

The invention discloses a DNA molecule identification method of scorpion medicinal materials in a Naoxintong preparation. According to the method, animal medicine components are digested by proteinaseK, and the plant components are extracted by a broad-spectrum plant genome DNA rapid extraction kit. Scorpion specific primers are designed based on mitochondrial cytochrome c oxidase subunit I (COI)for PCR amplification; and electrophoresis sequencing is conducted on the product, and result judgment is conducted on the obtained sequence in a GenBank database by using BLAST, wherein the speciescorresponding to the sequence with the highest similarity is the species closest to the sample to be detected. The specific primers used in the method can amplify a small fragment gene of about 250 bp, is not limited by the external form of scorpion, can identify a traditional Chinese medicine preparation containing scorpion powder, especially Naoxintong capsules, avoids the influence caused by DNA degradation, and has the characteristics of accuracy and universality. The quality control level of animal scorpion medicinal materials is improved, and the quality standard of Naoxintong capsules is further improved.