42 results about "Mitochondrial cytochrome" patented technology

The invention provides a method for rapidly identifying Lygus pratensis populations, especially Xinjiang populations, Gansu populations and Ningxia populations, by mitochondrial molecular markers. According to the method, specific primers are designed according to four gene segments including mitochondrial cytochrome oxidase II (COII), cytochrome b (Cytb), mitochondrial NADH dehydrogenase subunit 5(ND5) and 16S rDNA of Lygus pratensis respectively, and PCR (polymerase chain reaction) amplification products are subjected to series-connection comparison, so that identification for the Lygus pratensis populations in China is realized. The method is simple and reliable and has high operability, good stability and high repeatability.

The invention relates to a method for detecting the polymorphism of 12 loci of human mitochondrial cytochrome b genes simultaneously, and provides a method for detecting the polymorphism of 12 loci of mitochondrial cytochrome b genes (Cytochrome b, Cytb) simultaneously in the same system, which realizes the detection of the polymorphism of the 12 loci of the cytochrome cytb genes simultaneously in the same reaction system by combining the technology of polymerase chain reaction (PCR) with the technology of single basic group extension and fluorescence microarray hybridization. The fluorescence intensity and fluorescence type which are generated by single basic group extension only correspond to a genotype, and a polymorphic locus has only three kinds of characteristic fluorescence at most, and thus the genotypes of samples corresponding to all the same peak types can be obtained only by one-time DNA sequencing verification. Compared with a detection method by direct sequencing, the method of the invention has short time, namely experiments are finished within one day, and has high flux, namely the 12 polymorphic loci are detected directly in the same reaction; in addition, the method has low cost and visual result judgment.

The invention relates to a difunctional nanoprobe for detecting mitochondrial cytochrome C as well as a preparation method thereof. The preparation method comprises the following steps: taking a ligand of the mitochondrial cytochrome C; labeling fluorescein and aldehyde group on two ends of the ligand respectively; then adding magnetosome into the ligand again, and incubating overnight; then removing substances which do not react; then adding 1-(3-dimethyl amino propyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and methoxy-carboxylation-polyethylene glycol, and incubating overnight; and then removing substances which do not react, thereby obtaining the difunctional nanoprobe for detecting the mitochondrial cytochrome C. The difunctional nanoprobe and the preparation method thereof, which are provided by the invention, have good biocompatibility; the prepared probe for detecting mitochondrial cytochrome C has good dispersibility.

The invention belongs to a qualitative detection technology of animal-derived ingredients in feather down products and specifically relates to a primer / probe group for detecting a goose down component in the feather down product. A group of specific primer and probe is designed by selecting a mitochondrial cytochrome B (CYTB) gene sequence of goose down. By using the primer and the probe and adopting a real-time fluorescence PCR (polymerase chain reaction) technology, the goose down component in the feather down product can be fast, sensitively and specifically detected. The primer and the probe can be provided together with other reagents in the form of a kit and be used for nucleic acid amplification reaction. The method is simple and convenient to operate and good in repeatability.

The invention relates to a specific primer for amplifying the gene sequence of mitochondrial cytochrome c oxidase I (COI) of aleyrodidae insects. The sequence is represented by the SEQ ID No.1 and SEQ ID No.2. The provided amplification primer can rapidly and effectively amplify COI genes of multiple species of aleyrodidae insects, and thus can provide reliable references for the researches on growth relationship of aleyrodidae system, and population genetic differentials and genetic structure of specific insects.

A method is provided which, by applying a compound represented by formula (1) or an N-oxide or agriculturally acceptable salt thereof, controls soybean rust fungus having an amino acid substitution ofF129L in the mitochondrial cytochrome b protein (in the formula, Q represents a group represented by Q1, Q2, Q3, Q4 or Q5 (. represents the binding site to the benzene group), X represents an oxygenatom or NH; L represents CH2, anoxygen atom or NCH3;E represents a C6-C10 aryl group, etc.; R1 represents a C1-C3 chain hydrocarbon groupe, a cyclopropyl group, etc.; R2 represents a C1-C3 chain hydrocarbon group, a cyclopropyl group, etc.; R3 represents a C1-C3 alkoxy group or a C1-C3 chain hydrocarbon group; and n represents 0, 1, 2 or 3).

The present invention relates to a composition of bactericidal active compounds, and in particular to a composition comprising zinc 2-mercaptobenzothiazole and a mitochondrial cytochrome enzyme inhibitor (QoIs) bactericide and its formulation and use. A bactericidal composition comprising a bactericidal active compound I and a bactericidal active compound II. The bactericidal active compound I is zinc 2-mercaptobenzothiazole, and the bactericidal active compound II is at least one selected from mitochondrial cytochrome bactericides. Azoxystrobin, tristrostrobin, trifloxystrobin, pyraclostrobin, tristrostrobin, pyraclostrobin, tristrostrobin and cyanostrobin. The fungicidal active compound composition of the present invention can effectively slow down the development of drug resistance of diseases and greatly reduce the risk of resistance caused by the separate use of QoIs fungicide and 2-mercaptobenzothiazole zinc; the invention can also give full play to the QoIs fungicide and 2-mercaptobenzothiazole zinc Benzothiazole zinc has a preventive and therapeutic effect on fungal, bacterial and other diseases, as well as its good protective properties.

The invention belongs to the technical field of the detection of mouse original components, and particularly relates to a method for detecting the mouse original components by a specific primer. The craft process of method comprises the following five steps: specific primer design synthesis, DNA (Deoxyribonucleic Acid) extraction, reaction system establishment, PCR (Polymerase Chain Reaction) amplification and the analysis result evaluation of the mouse original components. The specific primer is designed and synthetized according to the mitochondrial cytochrome b gene sequence of a mouse in Genbank, wherein the base sequence of the forward primer F of the specific primer is 5'-GCCTTATAATTAATTGGAGGTA-3', and the base sequence of the downstream primer F of the specific primer is 5'-AGCTATATTATGTGCTTGAT-3'; on the basis of the mitochondrial cytochrome Cyt b gene sequence of the mouse, a real-time quantitative PCR method is used for detecting the mouse original components so as to bring good specificity and repeatability. When the real-time fluorescence quantitative PCR method carried out by the primer is used for detecting the mouse original components, operation is simple, short time is consumed, and specificity is high. Secondary pollution caused by product amplification when an amplification product is taken out to carry out electrophoresis identification in a traditional detection method is avoided, and a new approach is provided for the detection of the mouse original components.