Multiplex PCR method for distinguishing four meat components in food at the same time

Abstract

The invention belongs to the technical field of food quality and safety detection and particularly relates to a method for rapidly detecting pork, beef, mutton and chicken components in food at the same time through animal genomic DNA (deoxyribonucleic acid) extraction, primer design and UP-M-PCR (universal primers-multiplex-PCR). The four meat components can be distinguished rapidly at the same time according to differential sites of mitochondrial cytochrome b of the animal by using the forward primer sharing and reverse primer specificity strategy and an M-PCR system including five primers. The result shows that the method has the advantages that the detection limit can be up to the pictogram level, the components of the reaction system are free from cross interference and the meat products on market can be detected, verified and distinguished accurately by sampling 80 meat products randomly according to the specificity of the four target meat components. In a word, the invention provides a specific, sensitive and practical multiplex PCR method for rapidly screening four common meat components in food at the same time.

Description

1. Technical field [0001] The invention belongs to the technical field of food quality and safety detection, and in particular relates to a simultaneous and rapid detection method for four meat components of pigs, cattle, sheep and chickens in food by using a universal primer multiplex PCR method and an application thereof. 2. Background technology [0002] The adulteration of meat and meat products is one of the important challenges faced by my country's food quality control. Driven by profit, illegal enterprises use relatively cheap pork, chicken and other meat raw materials, and sell them as beef and mutton products, which seriously violates the legitimate rights and interests of consumers. The exposure of the "beef paste" incident has further brought meat adulteration into the focus of public attention. The traditional meat morphological identification methods relying on senses and experience are far from meeting the needs of controlling and supervising meat adulteratio...

AI Technical Summary

Problems solved by technology

However, at present, in view of the status quo of meat adulteration in my country, the rapid simultaneous identification and screening technology of meat using multiplex PCR has not been reported.

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