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STR typing and paternity testing method and system based on second-generation sequencing
PendingCN122598767ASolve problems that cannot be directly used to determine paternityGuaranteed reliability
The application belongs to the technical field of biology and genetics, and particularly relates to a STR typing parentage identification method and system based on second-generation sequencing. The method comprises the following steps: performing quality filtering on second-generation sequencing raw data to obtain clean data; aligning the clean data to a human reference genome hg38 to obtain an alignment file; based on the alignment file, analyzing the number of core repeat sequences and sequence structure of a target STR locus to obtain an allele typing result; checking the sequencing depth of each STR locus to be greater than or equal to 300x and the proportion of heterozygous allele reads to be between 40:60 and 60:60; aligning the allele typing results of the offspring and the putative parents one by one to verify whether the Mendelian inheritance law is met; calculating the paternity index of each STR locus, multiplying the cumulative paternity index, calculating the paternity probability, and outputting a conclusion supporting the parent-child relationship when the paternity probability is greater than 99.99%; and outputting a conclusion excluding the parent-child relationship when the cumulative paternity index is less than 0.0001.
Owner:SUZHOU HUAQIAN TECH CO LTD
Multichannel microfluidic label-free pathogen detection system and method with internal reference calibration
A multi-channel microfluidic label-free pathogen detection system and method with internal reference correction is disclosed, relating to the field of pathogen detection. The method includes: acquiring the initial interference spectrum of each functional detection region before the reaction; injecting the sample to be tested and distributing it to multiple parallel microfluidic channels according to a preset flow path; specific binding of the target pathogen to the aptamer to form an aptamer-pathogen complex layer; acquiring the post-reaction interference spectrum of each functional detection region, comparing the post-reaction interference spectrum with the initial interference spectrum before the reaction, and calculating the change in optical path difference of each functional detection region; calculating the target detection signal and comparing it with a standard curve to obtain the concentration of the target pathogen in the sample. This invention achieves continuous, accurate, and repeatable spectral acquisition of multiple functional detection regions, without the need for nucleic acid amplification or labeling, realizing multi-channel parallel, automated acquisition, internal reference correction, and quantitative analysis of pathogen concentration, thus improving the accuracy and reliability of the detection results.
Owner:CHANGCHUN UNIV OF SCI & TECH
Primer probe combination for identifying E, J and K subgroups of avian leukosis virus, detection method and application of primer probe combination
PendingCN121951138Ano cross reactionStrong specificityMicrobiological testing/measurementMicroorganism based processesLeucosisAvian leukosis viruses
The invention relates to a primer probe combination for identifying E, J and K subgroups of avian leukosis viruses, a detection method and application of the primer probe combination. The detection system for identifying the avian leukosis virus subgroup comprises an upstream primer ALV-E-F, a downstream primer ALV-E-R and a probe ALV-E-P of a gp85 gene conserved region of targeted ALV-E, and an upstream primer ALV-J-F, a downstream primer ALV-J-R and a probe ALV-J-P of an env gene conserved region of targeted ALV-J, the upstream primer ALV-K-F, the downstream primer ALV-K-R and the probe ALV-K-P are used for targeting a gp85 gene conserved region of ALV-K. The detection system for identifying the avian leukosis virus subgroups has no cross reaction with other ALV subgroups and common avian pathogens, and has strong specificity.
Owner:FOSHAN UNIVERSITY +1
Automatic molding equipment for cement strength test
PendingCN121989339ARealize unmanned operationHigh detection throughputDrying gas arrangementsFeeding arrangmentsMolding machineCement mortar
The invention discloses automatic molding equipment for cement strength testing, and relates to the technical field of cement production, the automatic molding equipment comprises an electric cabinet, a first bracket, a second bracket, a carrying unit, a stirring and pouring unit, a vibration unit and a slicking unit; the carrying unit is mounted on the first bracket and is used for carrying the cement mold; the stirring and pouring unit is mounted on the second bracket and is used for stirring, molding and pouring the cement; the vibration unit is installed on the advancing path of the carrying unit and located in front of the stirring and pouring unit. The cement mortar molding machine integrates mold carrying, automatic stirring and pouring, vibration molding, strickling and cleaning, and unmanned operation of the whole cement mortar molding process is achieved through collaborative operation of the carrying unit, the stirring and pouring unit, the vibration unit and the strickling unit; and through equipment height difference layout and process interpenetration, multi-batch molding parallel operation can be realized, the molding period is greatly shortened, and the laboratory detection flux is improved.
Owner:SHANGHAI ZHIZHI TECH CO LTD
Universal molecular beacon kit for various animal-derived components
PendingCN121852551AEfficient identificationMismatch resolution optimizationMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologygenomic DNA
The invention discloses a universal molecular beacon kit for various animal-derived components, and belongs to the technical field of molecular biological detection. Aiming at gene segments with specific structural characteristics in 11 common livestock and poultry animal genomes, the invention designs a composition of a universal primer and a molecular beacon fluorescent probe. The combination can be used for carrying out real-time fluorescent PCR detection and melting curve analysis by using the kit and the method by taking genome DNA of a sample to be detected as a template. By comparing the shape of a melting peak of a detected sample and the characteristic melting temperature (Tm value) of the detected sample, 11 animal-derived components of livestock and poultry can be quickly and accurately identified under the condition of single-tube detection. Technical innovation is achieved in the field of molecular biological detection, an efficient and reliable solution is provided for animal-derived component detection, and the method has important practical value and popularization potential.
Owner:NINGBO PROD & FOOD QUALITY INSPECTION INST (NINGBO FIBER INSPECTION INST) +1
Modular radio frequency detection device
The utility model relates to radio frequency circuit test technical field, more particularly to a kind of modular radio frequency detection equipment, comprising: host computer, host computer is provided with main control unit and addressing routing circuit in it;At least one slave, slave and the addressing routing circuit of host computer are connected through first communication bus;Host computer, host computer and the addressing routing circuit of host computer are connected through second communication bus;Wherein, addressing routing circuit includes: first data port, is connected to second communication bus;Second data port, is connected to first communication bus;And third data port, is connected to main control unit;Addressing routing circuit is embedded with the gate circuit for the address information in the data packet input according to first data port, and the physical data transmission path between first, second and third data port is conducted through.This utility model is through the configuration switching and parallel communication structure of hardware, realizes quick change type and high-throughput parallel detection.
Owner:CHENGDU EBYTE ELECTRONICS TECH CO LTD
Glass insulator hidden damage nondestructive testing device based on infrared thermal wave technology
The application discloses a glass insulator hidden damage nondestructive testing device based on infrared thermal wave technology, which comprises a machine box, an electric control box fixed on the outer wall of one side of the machine box, and a rotary table machine electrically connected with the electric control box and controlled by the electric control box; at least four jointed slave molds are distributed on the rotary table machine in a ring shape at equal intervals, and each jointed slave mold is used for bearing one glass insulator to be detected; a position detection assembly is installed at the top end of the machine box and electrically connected with the electric control box, and is used for detecting whether each jointed slave mold reaches a predetermined detection station. The application constructs a parallel station layout through the plurality of jointed slave molds on the rotary table machine, fundamentally changes the traditional mode that detection and auxiliary operation are performed in series, sets a reasonable step angle, covers all areas in the entire circumferential direction of the insulator, and effectively avoids missed detection caused by defects located on the back or the edge of the field of view during fixed posture detection.
Owner:JIANGXI ZHONGCI ELECTRIC CO LTD
Method for detecting copy number variation of STRC gene based on whole genome sequencing
ActiveCN116453588BExpanding the range of genetic diseasesEasy to detectWhole genome sequencingPseudogene
The application provides a STRC gene copy number variation detection method based on whole genome sequencing. The STRC gene and the STRCP1 gene are subjected to sequence alignment to find each difference site of the STRC gene and the STRCP1 gene. For each difference site, the sequence of the corresponding STRC position and STRCP1 position in the genome is read from a variation detection file. The total copy number of the true gene and the false gene is calculated by taking the reference site in the genome as a reference. The STRC gene copy proportion on each difference site is calculated. The STRC gene copy number on each difference site is calculated according to the total copy number and the STRC gene copy proportion. The STRC gene copy number on each exon is determined according to the STRC gene copy number on each difference site. The method can realize the detection of the STRC copy number, simplifies the detection process, improves the detection throughput and reduces the cost.
Owner:BGI GENOMICS CO LTD +1
Soil organic matter analysis device capable of processing samples in batches
ActiveCN224202862UEfficient removalavoid accumulationPreparing sample for investigationEarth material testingSoil organic matterAgricultural engineering
The utility model provides a soil organic matter analysis device capable of processing samples in batches, which belongs to the technical field of soil analysis and comprises a bearing mechanism, a working box, a driving mechanism arranged at the top of the working box and a cleaning component arranged at the bottom of the driving mechanism. According to the utility model, the multi-station turntable is matched with the eight groups of annularly arranged bearing tables to realize batch sample circulating treatment, so that the detection flux is obviously improved; a fan and an air blowing pipe head of the soot blowing assembly are combined with a rolling brush cylinder of the sweeping assembly to form a dual cleaning system, and viscous substances and cakes on the surface of the sample are effectively removed; a detection probe of the analysis assembly realizes multi-point automatic detection through a linear guide rail; a guide plate and a collecting box of the collecting mechanism clean residues in time, and dirt accumulation is avoided; the problem that traditional single sample detection efficiency is low is solved, automatic batch processing is achieved, meanwhile, equipment blockage is effectively prevented through multiple cleaning and a waste collecting system, the detection efficiency is remarkably improved, and the service life of the equipment is remarkably prolonged.
Owner:TIANJIN GEOLOGICAL & MINERAL TESTING CENTER CO LTD
A microfluidic chip integrating nucleic acid extraction and high-throughput ECL detection, and a preparation method and application thereof
ActiveCN116586129Beasy to operateshorten the timeBioreactor/fermenter combinationsBiological substance pretreatmentsNucleic acid detectionElectrochemiluminescence
The application discloses a micro-fluidic chip integrating nucleic acid extraction and high-throughput ECL detection, and a preparation method and application thereof. The micro-fluidic chip comprises a cover sheet, an intermediate layer and a substrate from top to bottom. The intermediate layer is provided with a nucleic acid extraction reagent storage bin, an electrochemical luminescence reagent storage bin, an electrochemical luminescence reaction bin and a waste liquid bin. The substrate is provided with a gold electrode corresponding to the electrochemical luminescence reaction bin. The preparation method comprises the following steps: substrate cleaning, nano-gold preparation, substrate gold electrode preparation, PDMS glue preparation and micro-fluidic chip construction. The application has the advantages that the operation process of nucleic acid extraction reduces external pollution, improves nucleic acid detection throughput, shortens nucleic acid extraction and detection time, reduces detection cost, and greatly reduces professional requirements for nucleic acid extraction and detection.
Owner:DERMATOLOGY HOSPITAL SOUTHERN MEDICAL UNIV (GUANGDONG PROVINCIAL DERMATOLOGY HOSPITAL GUANGDONG PROVINCIAL CENT FOR STI & SKIN DISEASES CONTROL & PREVENTION RES CENT FOR LEPROSY CONTROL & PREVENTION CHINA) +2
A multiplex PCR primer set and method for simultaneously detecting multiple symbiotic bacteria and viruses in bemisia tabaci
The application discloses a multiplex PCR primer set for simultaneously detecting multiple symbiotic bacteria and viruses in Bemisia tabaci, comprising the following: a primer pair for detecting TYLCV, the nucleotide sequences of which are shown as SEQ ID NO:1 and SEQ ID NO:2; a primer pair for detecting Rickettsia , the nucleotide sequences of which are shown as SEQ ID NO:3 and SEQ ID NO:4; a primer pair for detecting Cardinium , the nucleotide sequences of which are shown as SEQ ID NO:5 and SEQ ID NO:6; and a primer pair for detecting Hamiltonella , the nucleotide sequences of which are shown as SEQ ID NO:7 and SEQ ID NO:8. The application solves the technical problems of low detection efficiency, large sample consumption, high reagent cost, inconvenient data comparison and poor compatibility of primers in a multiplex PCR system in the prior art.
Owner:QINGDAO AGRI UNIV
Fluorescence immunoassay reagent card detection device
ActiveCN224190031UHigh degree of intelligenceImprove detection stabilityBiological testingFluorescence/phosphorescenceTelecommunicationsBiology
The utility model discloses a fluorescence immunoassay reagent card detection device, which comprises a protective shell, a reagent card transmission inlet, a reagent card transmission outlet, a fluorescence immunoassay reagent card detection module, a fluorescence immunoassay reagent card detection module, a fluorescence immunoassay reagent card detection module and a fluorescence immunoassay reagent card detection module, the plurality of conveying channels are fixedly arranged in the protective shell, are used for conveying the reagent cards to be detected, are arranged in parallel, are positioned on the same horizontal plane, and are respectively communicated with the reagent card conveying inlet and the reagent card conveying outlet; the optical assembly is fixedly arranged in the protective shell, is positioned right above the plurality of conveying channels, and faces the detection areas of the conveying channels so as to perform fluorescence signal detection on the plurality of reagent cards at the same time; and the driving assemblies are arranged in the protective shell and are arranged in a one-to-one correspondence manner along the lower parts of the plurality of conveying channels so as to drive the reagent cards to move along the conveying channels.
Owner:四川国际旅行卫生保健中心(成都海关口岸门诊部)
Molecular marker combination for identifying wax gourd germplasm resources and application of molecular marker combination
PendingCN122081538AHigh typing qualityHigh polymorphism
The invention belongs to the technical field of molecular biology, and discloses a molecular marker combination for identifying wax gourd germplasm resources, the molecular marker combination comprises 48 SNP molecular markers, the physical positions of the 48 SNP molecular markers are determined by sequence alignment based on a wax gourd reference genome GCF009727055.1, and the chromosome positions and base variation of the 48 SNP molecular markers are shown in the specification table 1. The invention further discloses a primer group for detecting the molecular marker combination, the primer group comprises 48 pairs of primers, and the sequence of each pair of primers is shown in the specification table 2. The invention also discloses a kit which comprises the primer group. The invention also discloses applications of the molecular marker combination, the primer group and the kit. According to the molecular marker combination for identifying the wax gourd germplasm resources, 48 SNP markers are screened, and the 48 molecular markers are high in typing quality, single in copy and high in polymorphism.
Owner:HUNAN VEGETABLE RES INST +2
A chip for identifying polysaccharides in traditional Chinese medicine containing the structure →4)-β-D-Manp-(1→ and its application.
PendingCN122283122AAchieving Parallel Detectionimprove scienceBinding siteRelative fluorescence units
This invention relates to a chip for identifying polysaccharides from traditional Chinese medicine containing the structure →4)-β-D-Manp-(1→ and its application, belonging to the field of biochip technology. The chip of this invention comprises a solid-phase support and a microarray system, wherein the microarray system is fixed on the surface of the solid-phase support. Using a glass substrate treated with hydrazide as the carrier, the invention precisely spots the target polysaccharide, polysaccharide standards, and negative / positive controls using a spotting instrument to construct a polysaccharide chip containing 16 subarrays. After blocking the non-specific binding sites of the chip with a blocking buffer, fluorescently labeled LM21 antibody is added to the subarrays for incubation. By collecting fluorescence signals, the relative fluorescence unit value is obtained, directly reflecting the binding strength between the polysaccharide and the LM21 antibody. This invention is simple to operate, has high detection sensitivity, and strong structural recognition specificity, and can be widely used for the rapid screening and precise structural characterization of active polysaccharides from traditional Chinese medicine, providing an efficient technical means for the development and utilization of polysaccharides from traditional Chinese medicine.
Owner:NAT INST FOR FOOD & DRUG CONTROL
KASP molecular markers related to stem thickness of soybean and application thereof
This invention discloses a KASP molecular marker related to the thickness of soybean main stem and its application, belonging to the field of molecular genetics and breeding technology. The nucleotide sequence of the KASP molecular marker is shown in SEQ ID NO.1, and a T / C base mutation exists at position 26 of the sequence shown in SEQ ID NO.1. Compared with traditional markers, the development of the KASP molecular marker of this invention significantly improves the detection throughput, allowing a large number of samples to be processed in a single reaction, greatly improving the efficiency of genotyping and breeding selection; it can accurately distinguish SNP variations, and the genotyping results are accurate and reliable.
Owner:YANGZHOU UNIV
A molecular marker primer of pollock and its application
The application discloses a molecular marker primer for a narrow cod and application thereof, and relates to the technical field of molecular biology markers. The primer combination is composed of three pairs of primers, namely, GC6:TC14, GC8:GT15 and GC9:TG9, and the sequences of the primers are shown as SEQ ID NO. 1-6. The application develops, for the first time, the STR molecular marker primer combination specially used for sea area source identification of the narrow cod. Compared with the microsatellite marker used only for the population genetics basic research of the narrow cod in the prior art, the primer combination is optimized through multi-stage screening, can effectively distinguish the narrow cod samples from the Okhotsk Sea FAO 61 sea area and the Bering Sea FAO 67 sea area, and fills the technical blank in the sea area tracing field of the cross-border trade of the narrow cod.
Owner:SCIENCE & TECHNOLOGY RESEARCH CENTER OF CHINA CUSTOMS +1
Magnetic immune micro-fluidic chip
ActiveCN224086775Ueasy to distinguishImprove sealingLaboratory glasswaresMechanical engineeringMaterials science
The utility model discloses a magnetic immune micro-fluidic chip which comprises an upper chip layer, a middle glass substrate and a lower bottom plate, a plurality of detection channels are formed in the chip layer in the width direction, each detection channel comprises a capture area, and a sample inlet channel and a sample outlet channel are formed in the two sides of each capture area respectively; the sample inlet channel is provided with a sample inlet corresponding to the surface of the chip layer, and the sample outlet channel is provided with a sample outlet corresponding to the surface of the chip layer; and magnets are fixed on the bottom plate in positions corresponding to the capturing areas. The bottom plate of the chip is bonded and sealed by the tempered film, so that the chip has the advantages of good sealing performance, good fluorescence permeability, small thickness and the like. The bottom plate is detachably installed, the repeated utilization rate is higher, the chip layer is prone to blockage or cross contamination due to the fact that the caliber of the microfluidic channel is small, the upper side part can be detached, and the bottom plate and the magnet can be repeatedly used.
Owner:HEFEI MATERNAL & CHILD HEALTH HOSPITAL
Early warning evaluation method based on canceration caused by pulmonary nodule immune microenvironment disorder
PendingCN121885172AEasy to get materialsreduce dosageHealth-index calculationDisease diagnosisPulmonary noduleAutoantibody
The invention discloses an early warning evaluation method based on canceration caused by pulmonary nodule immune microenvironment imbalance, which comprises the following steps: S1, acquiring autoantibody detection data of normal, pulmonary nodule and lung cancer people from a database, and establishing a pulmonary nodule canceration data set Panne l containing 159 autoantibodies as risk early warning factors of pulmonary nodule canceration; s2, preparing a lung cancer risk factor Panne l protein chip according to the Panne l data, wherein the lung cancer risk factor Panne l protein chip comprises antigen proteins of 159 lung cancer related autoantibodies; s3, using a Panne l protein chip to detect the serum of the crowd; and S4, according to the detection data, establishing a risk early warning model of pulmonary nodule canceration for the analysis and early warning of the lung cancer occurrence risk of the crowd (mainly aiming at the pulmonary nodule crowd). According to the method, the cancer related autoantibodies are systematically detected through a protein chip method for people with high detection rate of pulmonary nodules in China, and the method is used for early warning of the occurrence risk of pulmonary nodule immune abnormality canceration of people.
Owner:GUANGZHOU RENXIN MEDICAL TECHNOLOGY CO LTD
Cigarette suction resistance detection device
ActiveCN224682047UHigh detection throughputAchieve positioningElectric machineryElectrical and Electronics engineering
Owner:HONGYUN HONGHE TOBACCO (GRP) CO LTD
Ultra-sensitive quantitative detection method of tumor driver genes based on digital PCR
The application discloses a tumor driving gene super-sensitive quantitative detection method based on digital PCR and belongs to the technical field of tumor gene detection in molecular biology. The application realizes accurate calculation of the average number of molecules in a microdroplet, the absolute concentration of nucleic acid and the frequency of variant alleles by means of directional pretreatment of clinical samples, targeted nucleic acid purification, multiple chemical modification of tumor driving gene primers and probes, microfluidic vibration atomization nanoscale microdroplet partitioning, six-color fluorescence channel multiple digital PCR amplification and machine learning assisted microdroplet intelligent typing. The application breaks through the bottleneck of existing digital PCR detection technology, avoids conventional processes and algorithms, realizes 0.001% VAF super-sensitive detection, has a quantitative relative deviation of less than or equal to 1%, simultaneously detects eight driving genes in a single tube, is suitable for multiple clinical samples such as plasma, FFPE tissue and body fluid, and is excellent in detection sensitivity, specificity and repeatability, and is suitable for early tumor screening, efficacy monitoring, recurrence early warning and targeted drug guidance.
Owner:CLAYYURE GENE TECH CO LTD
Method and system for rapidly determining residual quantity of meconazole enantiomers in watermelon sample
PendingCN121955240Aeliminate distractionsReduced Sensitivity RequirementsComponent separationWater methanolLinear correlation
The invention relates to the technical field of pesticide residue detection, in particular to a method and a system for rapidly determining the residual quantity of meconazole enantiomers in a watermelon sample. A sample is subjected to acetonitrile extraction and solid-phase extraction column purification, Acquity Trefoil CEL1 (150 mm * 3.0 mm, 2.5 [mu] m) separation is adopted, a supercritical carbonate-0. 5% (v / v) ammonia water methanol solution is adopted as a mobile phase, gradient elution is performed at the flow speed of 1.0 mL / min, and when the detection wavelength is 230 nm, the separation effect of the two meconazole enantiomers is the best. The linear correlation coefficient of the two meconazole enantiomers in the range of 0.5-20.0 mg / L is greater than 0.9998, and the limit of quantitation (RS / N = 10) of the method is 0.1 mg / kg. When the meconazole enantiomer in watermelons is in a range of 0.1 mg / kg to 1.0 mg / kg, the adding standard recovery rate is 80.2 percent to 105 percent, and the relative standard deviation is 2.8 percent to 7.8 percent. The method realizes the residue determination of the splitting of the meconazole enantiomer in the watermelon sample.
Owner:HANGZHOU CUSTOMS TECHNICAL CENTER +1
Method and kit for detecting anti-stem cell antibody
PendingCN122084887Ahigh sensitivityHigh detection throughputBiological testingAntiendomysial antibodiesCell biology
The invention provides an anti-stem cell antibody detection method and a kit. According to the method provided by the invention, the anti-stem cell antibody can be detected by adopting an MSD platform, and magnetic beads can be used for replacing stem cells to serve as an immunosuppression competition confirmation reagent to carry out a confirmation experiment. The detection method based on the MSD platform provided by the invention can improve the sensitivity of the method for detecting the stem cell antibody and / or improve the detection flux.
Owner:UNITED POWER PHARMA TECH CO LTD
Method for detecting volatile and semi-volatile organic compounds in heated cigarette aerosol and application thereof
PendingCN121878071AHigh detection throughputImprove analysis efficiencyComponent separationAir quality improvementInternal standardSorbent
The invention provides a method for detecting volatile and semi-volatile organic compounds in aerosol of a heated cigarette and application of the method. The method for detecting the volatile and semi-volatile organic compounds in the aerosol of the heated cigarette comprises the following steps: (1) smoking the heated cigarette to be detected, and trapping the aerosol by using a filter disc and an adsorbent; (2) mixing the filter disc capturing the aerosol, the adsorbent, an internal standard and a solvent, and extracting to obtain an extract; and (3) carrying out gas chromatography-tandem mass spectrometry detection on the extract, and obtaining the content of volatile and semi-volatile organic compounds in the heated cigarette to be detected according to the detection result. The method provided by the invention can simultaneously separate and analyze the content of volatile and semi-volatile organic compounds in various aerosols, improves the detection flux, improves the analysis efficiency, simplifies the pretreatment process, has the advantages of high flux, good peak shape, high separation degree, high sensitivity, good recovery rate and the like, and is suitable for industrial production. The method is suitable for analysis and determination of main volatile and semi-volatile organic compounds in heated cigarette aerosol.
Owner:CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
SNP molecular marker combination for breeding high-puffed corn and application thereof
This invention discloses a SNP molecular marker combination for breeding high-expansion-multiplicity popcorn and its application, belonging to the field of molecular breeding technology. The SNP molecular marker combination of this invention consists of 30 high-effect SNP loci located on the maize B73 reference genome v4 version. This combination was selected by performing genome-wide association analysis on 399 natural popcorn accessions and ranking the top 30 loci according to their SNP effect values. This invention allows for accurate prediction of the expansion-multiplicity trait by detecting the genotypes of these 30 SNP markers in the early stages of breeding, with a prediction accuracy of over 0.72. It has significant advantages such as high selection efficiency, short cycle, low cost, and insensitivity to environmental influences, and is suitable for molecular marker-assisted breeding and multi-trait aggregation breeding of popcorn.
Owner:SHANGHAI ACAD OF AGRI SCI
Multi-channel integrated capillary electrophoresis chip and flow path control method
ActiveCN121762658BHigh detection throughputImplement parallel detectionBioreactor/fermenter combinationsBiological substance pretreatmentsElectrophoresesCapillary electrophoresis
The application discloses a kind of multi-channel integrated capillary electrophoresis chip and flow path control method, belong to detection chip technical field.It includes: chip ontology, which is provided with sample inlet through hole, separation channel, plunger valve port, plunger valve channel;Sample inlet through hole is communicated with separation channel and is used for sample introduction;Separation channel is provided with multiple, any one separation channel is configured with a sample inlet through hole, plunger valve channel is communicated with separation channel by plunger valve port;Separation channel includes straight line section, first curve extension section and second curve extension section, and the total length of the straight line section, the first curve extension section and the second curve extension section of each separation channel is the same.Through integrating multiple separation channels with equal total length, each channel is equipped with independent sample inlet through hole and detection window, multi-channel synchronous detection is realized, and the unit length electric field intensity of each separation channel is consistent, effectively eliminates migration time drift, and improves multi-channel detection consistency.
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD
An egg spectrum acquisition device based on pre-scan
ActiveCN224399249UHigh detection throughputColor/spectral properties measurementsPhotovoltaic detectorsPhoto irradiation
The utility model discloses a kind of egg spectrum acquisition device based on pre-scanning, further include optical breadboard, optical breadboard is equipped with pre-position mechanism, the both sides of pre-position mechanism are provided with light source and dark box pillar, the upper end of dark box pillar is equipped with dark box, and the inside of dark box pillar is provided with spectrometer mechanism, the lower end of dark box is equipped with several light holes, light hole is used to place egg, and the inside wall of dark box is equipped with lens a and photoelectric detector, lens a is located between egg and photoelectric detector. By setting pre-position mechanism and spectrometer mechanism before light irradiation to egg, make light before entering egg carries out spectrometer, replace after egg emission and then carry out spectrometer, so as to replace spectrum camera by low-cost photoelectric detector and optical filter, the data acquisition card of acquisition end can directly collect corresponding wavelength light, and also can be expanded detection array by adding lens and photoelectric detector, so as to improve detection flux.
Owner:TIANFU JIANGXI LAB
Protein and RNA multiple in-situ detection method based on nucleic acid coupling antibody and in-situ hybridization
PendingCN121951009Aimprove throughputlow costMicrobiological testing/measurementBiological testingDiseaseProtein target
The invention belongs to the technical field of molecular biology in-situ detection, and particularly relates to a protein and RNA multiple in-situ detection method based on a nucleic acid coupling antibody and in-situ hybridization. According to the method, firstly, an antibody coupled with a specific nucleic acid tag is combined with a target protein, then the nucleic acid tag carried by the antibody is detected through a fluorescence in-situ hybridization technology, and in-situ detection of nucleic acid molecules is synchronously achieved; in the aspect of signal interpretation, a unique signal coding and interpretation mode is used, and high-throughput space co-detection of protein and nucleic acid on a single sample is realized. The method effectively breaks through the limitation of secondary antibody species sources in traditional immunodetection, overcomes the technical bottleneck that multiple in-situ analysis of protein and nucleic acid is difficult to perform in the same sample, and can synchronously obtain spatial distribution and expression information of target protein and nucleic acid in a single sample. The invention provides a powerful technical tool for life science research, disease pathological diagnosis, drug screening and treatment guidance.
Owner:KUNYU (SHENZHEN) BIOTECHNOLOGY CO LTD
Simultaneous ionization of organic and heavy metal elements by electrospray extraction-microwave plasma ion source
The application discloses a kind of organic matter and heavy metal element simultaneously ionization spray extraction-microwave plasma ion source, comprising: three-way pipe, the three-way pipe includes vertically arranged ionization channel and extraction channel;The side of the ionization channel is equipped with microwave plasma source, the other side is connected with mass spectrometer;Sample introduction joint, sample solution is injected into sample introduction joint, and the sample gas obtained after being atomized by auxiliary gas is introduced into extraction channel;Extraction liquid introduction joint, mixed extraction liquid is injected into extraction liquid introduction joint, and the extraction gas obtained after being atomized by auxiliary gas is introduced into extraction channel and mixed with sample gas, and the mixed extraction liquid includes EDTA and at least one of methanol or acetonitrile.The technical scheme is fast, the operation step is simple, the sample detection flux can be improved, and has very high practicality and economic benefits in actual application.
Owner:SUZHOU ZHIPU WEIXING INTELLIGENT TECH CO LTD
An rt-lamp virus nucleic acid semi-quantitative detection method and kit based on split freeze-dried microspheres and multi-channel microfluidic chip
This invention discloses a semi-quantitative RT-LAMP method and kit for viral nucleic acid detection based on dispensed lyophilized microspheres and a multi-channel microfluidic chip. The method includes: designing two independent LAMP primer sets (P1 and P2) for each of the SARS-CoV-2 Omicron strain N gene, Zika virus NS5 gene, H1N1 influenza virus M1 gene, and H3N2 influenza virus NP gene; preparing two independent types of lyophilized microspheres by independently mixing RT-LAMP premix containing Bst DNA polymerase and other components with LAMP primer mixture and a lyophilization protectant; and using an N-channel microfluidic chip to perform serial dilutions and parallel RT-LAMP amplification of the same sample, determining the concentration of viral nucleic acid in the sample by the positive / negative inflection points of each channel. This invention is simple to operate, requires no equipment such as a real-time PCR instrument, and is suitable for rapid virus detection in various scenarios.
Owner:SOUTH CHINA UNIV OF TECH +1
Rapid detection method for migration quantity of chloropropanol compounds in cigarette paper
PendingCN121978243AAvoid the impact of test duplicationeasy to operateComponent separationPolyethylene glycolDerivatization
The invention belongs to the technical field of material analysis, and provides a method for rapidly detecting the migration quantity of chloropropanol compounds in cigarette paper, which comprises the following steps: carrying out a water phase migration experiment on a cigarette paper sample to be detected to obtain a migration liquid; adding deuterated chloropropanol into the migration liquid, mixing with an internal standard stock solution, and carrying out salting-out auxiliary liquid-liquid extraction, dehydration purification and nitrogen blowing concentration to obtain a liquid to be detected; a gas chromatography-tandem mass spectrometry method is adopted to measure the to-be-measured liquid, a chromatographic column is an ultra-high inert bonded polyethylene glycol capillary chromatographic column, and data are collected in a multi-reaction monitoring mode; the migration amount of the chloropropanol compounds in the sample is calculated through an internal standard method, and the chloropropanol compounds comprise 1, 3-dichloro-2-propanol and 3-chloro-1, 2-propylene glycol. The method does not need a derivatization step, is high in detection accuracy, and can realize rapid detection and batch analysis.
Owner:CHINA TOBACCO YUNNAN IND