97results about How to "High detection throughput" patented technology

The invention relates to the technical field of biological detection, and provides an inflammatory factor detection kit based on a liquid-based chip technology, and the inflammatory factor detection kit comprises six fluorescent microspheres coupled with capture antibodies, and six detection antibodies labeled by report fluorescence; in the six fluorescent microspheres containing the captured antibodies, the fluorescence intensities of all the fluorescent microspheres are different; according to the detection kit provided by the invention, on the basis of a liquid-based chip technology, fluorescein is adopted to carry out fluorescence coding on microspheres, and a captured antibody is connected to the fluorescent microspheres through a chemical reaction; a detection target is captured through the fluorescent microspheres, and then a signal is displayed through the detection antibody, so that the detection of the inflammatory factors is completed, the defects of large demand quantity of soluble inflammatory factor detection samples, long detection time, tedious detection steps and the like in the prior art are overcome, and great convenience is brought to scientific researchers at a hospital end and an industrial end.

The invention discloses a kit for rapidly detecting respiratory pathogens by combining multiple PCR (Polymerase Chain Reaction) with CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (Clustered Regularly Interspaced Short Palindromic Repeats). The kit comprises a multiple amplification primer combination, an sgRNA detection plate, DNA (Deoxyribose Nucleic Acid) polymerase, RNA reverse transcriptase, CRISPR / Cas The sequence of the multiple amplification primer combination is as shown in SEQ NO: 1-374; the sgRNA detection plate is an array type microporous plate, and each detection hole is filled with one sgRNA as shown in SEQ NO 383-569. The kit disclosed by the invention can be used for nucleic acid detection of respiratory pathogens and can be used for detecting viruses and bacteria at the same time, and the detection flux reaches up to 178 pathogens; the CRISPR-Cas is used for carrying out specific recognition on nucleic acid fragment targets in an amplification product, fluorescence detection is carried out, and the method has the advantages of being ultra-multiple, low in equipment requirement, low in cost, short in time, high in specificity, high in sensitivity and the like, and can provide molecular level judgment basis and help for clinical diagnosis of pathogen infection.

The invention provides a kit and a method for simultaneously detecting 11 pathogens of a urogenital system and application, and belongs to the field of molecular biology. The primer group is used for detecting urogenital tract pathogens, and the urogenital tract pathogens comprise ureaplasma urealyticum (UU), chlamydia trachomatis (CT), mycoplasma genitalium (MG), ureaplasma parvum (UP), mycoplasma hominis (MH), adenovirus (ADV), trichomonas vaginalis (TV), herpes simplex virus type I (HSV-I), herpes simplex virus type II (HSV-II), haemophilus influenzae (HI) and neisseria gonorrhoeae (NG). The primer group is used for multi-joint detection of non-gonococcal urethritis related pathogens, has high specificity and sensitivity, is adaptive to non-invasive urine specimens, and supports home self-sampling. The kit is used for clinical diagnosis and can quickly judge pathogen types. Precise medication is performed based on a detection result, the healing time of a patient is shortened, the burden of a doctor is relieved, and meanwhile the pathogen transmission risk is reduced.

The invention belongs to the technical field of micro-fluidic chips, and discloses a hepatic lobule chip which comprises a single cell capture array chip, a micropore array chip and an antibody slide, a capture array on the single cell capture array chip and a micropore array on the micropore array chip are in a polygon shape, perfusion culture solution channels are arranged in the diagonal directions of all vertexes of the polygon, and the capture array and the micropore array are in one-to-one correspondence after being assembled. The liver lobule chip overcomes the dependence of research on spatial heterogeneity of liver cells on precise liver anatomical slices, avoids the defects that the liver slices are difficult to obtain and store, can directly reveal spatial distribution characteristics of secretion activity of the liver cells in the liver lobule, and becomes an ideal tool for spatial heterogeneity secretion of the liver cells. The invention further discloses application of the hepatic lobule chip in hepatic lobule spatial heterogeneity single cell secretion analysis, operation is easy, large experimental instruments are not needed, cost is low, and the hepatic lobule chip is easy to master.

A space transcriptome in-situ sequencing method for tissue slices relates to the field of gene sequencing, and comprises the following steps: 1) capturing mRNA in situ; 2) carrying out reverse transcription to obtain cDNA; (3) removing tissues and retaining cDNA (complementary deoxyribonucleic acid); 4) carrying out cDNA probe hybridization; 5) performing in-situ amplification; 6) decoding and sequencing the fluorescent probe; and 7) cleaning the probe, and sequencing for multiple rounds. According to the invention, RNA is captured in situ and is reversely transcribed into cDNA, nucleic acid imprinting transfer is realized, background interference is removed, the problem of RNA degradation is solved, and multiple rounds of hybridization are allowed to increase the detection flux. The nucleic acid sequence is covalently bound with a slide to prevent a signal point from shifting and falling off. A DNA coding padlock probe is used for identifying genes, amplifying and amplifying signals, and gene expression is revealed through sequencing and decoding. After the probe is cleaned, the probe is replaced for hybridization amplification, so that the flux is improved, and space crowding and fluorescence signal crowding are reduced.

The invention provides a digital liquid drop micro-fluidic chip system and a bacteria detection method, and relates to the technical field of microbiological detection, the digital liquid drop micro-fluidic chip system comprises a micro-fluidic liquid drop generation chip, a micro-fluidic liquid drop fusion chip and a micro-fluidic liquid drop reaction chip. The bacterium detection method comprises the step of carrying out bacterium detection on a sample by using the digital droplet micro-fluidic chip system. The digital droplet micro-fluidic chip system and the bacterium detection method have the advantages of high detection flux, high stability, high sensitivity, rapidness, low cost and the like, are suitable for simultaneous detection of various bacteria in unknown samples, and can be used in the fields of environmental microorganism detection, food-borne pathogenic bacterium detection, clinical pathogenic bacterium diagnosis and the like.

The invention discloses a primer probe composition for detecting eight gene mutations of human gliocytoma and application of the primer probe composition. The primer probe composition comprises primers and probes for detecting IDH1, IDH2, TERT promoters, H3F3A, H3C2, BRAF, EGFR and MET genes and a distribution mode of the primers and the probes. By screening and combining multiple primers, a primer combination with the minimum mutual interference degree is determined, the specificity and sensitivity of the primers are improved, the gene mutation low-frequency variation detection performance (VAF) reaches 0.2%, the gene fusion reaches the detection limit of 30 copies, and the detection of 6 gene mutations and 2 gene fusion can be completed at a time by applying a fluorescent PCR method. The method can be used for detecting tissue samples and cerebrospinal fluid samples.

The invention discloses an LSPR (Localized Surface Plasmon Resonance) detection device and detection method based on wavelength phase co-modulation, and the device utilizes a liquid crystal variable delayer to carry out phase delay on polarized light so as to realize sinusoidal modulation of emergent light intensity. The difficulty of demodulating the LSPR phase signal is reduced, the acquisition speed of the LSPR phase signal is improved, and the detection sensitivity is greatly improved. A light intensity image is recorded through the area array detector, and each pixel of the area array detector corresponds to one sensing channel, so that the detection flux is effectively improved. The optical filter is used for carrying out tunable filtering on incident light, controllable wide-field excitation of the LSPR sensing chip with different wavelengths is realized, crosstalk among different spectrums caused by strong scattering of the LSPR nano-chip due to simultaneous excitation of a broadband light source is avoided, and the accuracy of the LSPR sensing technology is further improved. Moreover, a plurality of suitable wavelengths are spliced, and phase detection is performed on a specified site, so that the dynamic detection range of the LSPR phase signal is effectively expanded.

The invention relates to the technical field of molecular interaction analysis, in particular to a method for analyzing intermolecular interaction, which comprises the following steps of: adopting a rare earth material with ligand molecules modified on the surface as an up-conversion material, contacting the up-conversion material with target molecules in a solution system, and irradiating the solution system by using exciting light matched with the rare earth material to obtain the intermolecular interaction. The intensity of the emitted light is changed by continuously changing the intensity of the exciting light actually received by the up-conversion material in the dynamic process of continuously combining and dissociating target molecules with ligand molecules of the up-conversion material, and a molecular interaction dynamic constant can be calculated by recording a change curve of the intensity of the emitted light along with time. According to the present invention, the BLI technology has the binding rate constant (Kon), the dissociation rate constant (Koff), the affinity (KD) and the like, and the technical problems of low precision of the existing BLI technology, expensive SPR technology and the like are solved. Based on the principle, the invention also develops a series of experimental consumables, kits, sensors and analysis devices for molecular interaction analysis.

The invention discloses a KASP molecular marker, a detection method and an application of a wheat leaf rust resistance gene Lr16G216 in the adult stage, and belongs to the field of molecular breeding technology. The KASP molecular markers tightly linked to the wheat leaf rust resistance gene Lr16G216 in the adult stage are Ax109403980 and Ax95083494, and the above two molecular markers are co-localized with the wheat leaf rust resistance gene Lr16G216 in the adult stage on the long arm of wheat chromosome 6B, and the genetic distance between the two is 0.59cM. Compared with conventional molecular markers, the molecular markers provided by the invention can not only efficiently, accurately and high-throughput detect the genetic mapping population of Lr16G216, and be applied to the fine positioning and map cloning of Lr16G216, but also can perform high-throughput molecular marker-assisted selection, realize high-throughput screening of the disease resistance gene Lr16G216 in a large breeding population in a short time, and can effectively improve the efficiency of molecular breeding for wheat leaf rust resistance.

The invention belongs to the technical field of fish genetic identification, discloses construction of a large yellow croaker wild and domesticated population typing identification method based on 10 universal SNPs sites, and particularly discloses a group of SNP molecular markers for screening large yellow croaker wild and domesticated samples. The invention provides a group of SNP molecular markers for discriminating wild and domesticated samples of pseudosciaena crocea, and by detecting the SNP molecular markers, the identification of the wild and domesticated samples of the pseudosciaena crocea can be realized by only needing a multiple PCR reaction system and SNaPshot typing sequencing. The method can realize automatic typing and identification of large yellow croaker wild samples and large yellow croaker domestication samples only by operating on various genetic analysis instruments. Compared with the current method for identifying the pseudosciaena crocea population, the method provided by the invention has the advantages of quicker operation and higher detection flux, and more importantly, the method has extremely high accuracy and cumulative exclusion rate.

The application belongs to the technical field of biology and genetics, and particularly relates to a STR typing parentage identification method and system based on second-generation sequencing. The method comprises the following steps: performing quality filtering on second-generation sequencing raw data to obtain clean data; aligning the clean data to a human reference genome hg38 to obtain an alignment file; based on the alignment file, analyzing the number of core repeat sequences and sequence structure of a target STR locus to obtain an allele typing result; checking the sequencing depth of each STR locus to be greater than or equal to 300x and the proportion of heterozygous allele reads to be between 40:60 and 60:60; aligning the allele typing results of the offspring and the putative parents one by one to verify whether the Mendelian inheritance law is met; calculating the paternity index of each STR locus, multiplying the cumulative paternity index, calculating the paternity probability, and outputting a conclusion supporting the parent-child relationship when the paternity probability is greater than 99.99%; and outputting a conclusion excluding the parent-child relationship when the cumulative paternity index is less than 0.0001.

A multi-channel microfluidic label-free pathogen detection system and method with internal reference correction is disclosed, relating to the field of pathogen detection. The method includes: acquiring the initial interference spectrum of each functional detection region before the reaction; injecting the sample to be tested and distributing it to multiple parallel microfluidic channels according to a preset flow path; specific binding of the target pathogen to the aptamer to form an aptamer-pathogen complex layer; acquiring the post-reaction interference spectrum of each functional detection region, comparing the post-reaction interference spectrum with the initial interference spectrum before the reaction, and calculating the change in optical path difference of each functional detection region; calculating the target detection signal and comparing it with a standard curve to obtain the concentration of the target pathogen in the sample. This invention achieves continuous, accurate, and repeatable spectral acquisition of multiple functional detection regions, without the need for nucleic acid amplification or labeling, realizing multi-channel parallel, automated acquisition, internal reference correction, and quantitative analysis of pathogen concentration, thus improving the accuracy and reliability of the detection results.

The microfluidic device comprises a substrate, a micro-channel layer, a porous microelectrode array and a cell culture layer which are sequentially arranged from bottom to top, the micro-channel layer is fixed to the substrate, at least part of the porous microelectrode array is fixed between the micro-channel layer and the cell culture layer, and the cell culture layer is fixed to the micro-channel layer; a first through hole and a second through hole are formed in the cell culture layer, a first blind hole, a second blind hole and a microfluidic channel are formed in one surface, close to the cell culture layer, of the microfluidic channel layer, two ends of the microfluidic channel are communicated with the first blind hole and the second blind hole respectively, the first through hole is aligned with the first blind hole, and the second through hole is aligned with the second blind hole. A plurality of third through holes are formed in the part, located between the micro-channel layer and the cell culture layer, of the porous microelectrode array, the first through holes and the first blind holes are communicated with each other through at least part of the third through holes, and the second through holes and the second blind holes are aligned and communicated with each other.

The invention relates to a primer probe combination for identifying E, J and K subgroups of avian leukosis viruses, a detection method and application of the primer probe combination. The detection system for identifying the avian leukosis virus subgroup comprises an upstream primer ALV-E-F, a downstream primer ALV-E-R and a probe ALV-E-P of a gp85 gene conserved region of targeted ALV-E, and an upstream primer ALV-J-F, a downstream primer ALV-J-R and a probe ALV-J-P of an env gene conserved region of targeted ALV-J, the upstream primer ALV-K-F, the downstream primer ALV-K-R and the probe ALV-K-P are used for targeting a gp85 gene conserved region of ALV-K. The detection system for identifying the avian leukosis virus subgroups has no cross reaction with other ALV subgroups and common avian pathogens, and has strong specificity.

The invention discloses a mass spectrum identification method for a human recombinant erythropoietin source, which comprises the following steps: S1, preparing a biotin-bound erythropoietin antibody A, and binding the erythropoietin antibody A with EPO (erythropoietin) in a sample to obtain a mixture 1; s2, performing co-incubation on magnetic beads coated with streptavidin and the mixture 1 to realize purification of erythropoietin; s3, carrying out reductive alkylation and trypsin digestion on the purified erythropoietin; and S4, carrying out qualitative detection on the peptide fragment of the erythropoietin subjected to enzyme digestion by adopting a mass spectrometer. According to the method, the characteristic glycopeptide containing the characteristic sialic acid in the CHO cell-derived rhEPO is detected by utilizing a mass spectrometry technology, and the method has good accuracy. Compared with a general immunological detection method, the method provided by the invention has higher detection flux, and provides a new thought and direction for rhEPO stimulant screening and rhEPO source identification in the biological medicine field.

The present invention discloses an ELISA kit for detecting anti-NXP2 antibodies, characterized in that it comprises: a purified dominant antigen epitope NXP2 protein, wherein the amino acid sequence corresponding to the purified dominant antigen epitope NXP2 protein is shown in SEQ NO.1. The present invention also discloses a detection method and application thereof. The kit of the present invention can be used for anti-NXP2 antibody detection, fills the gap in the anti-NXP2 antibody detection method, has the characteristics of large detection flux, accurate results, high sensitivity, and simple operation, and is easier to promote and apply.

The invention discloses automatic molding equipment for cement strength testing, and relates to the technical field of cement production, the automatic molding equipment comprises an electric cabinet, a first bracket, a second bracket, a carrying unit, a stirring and pouring unit, a vibration unit and a slicking unit; the carrying unit is mounted on the first bracket and is used for carrying the cement mold; the stirring and pouring unit is mounted on the second bracket and is used for stirring, molding and pouring the cement; the vibration unit is installed on the advancing path of the carrying unit and located in front of the stirring and pouring unit. The cement mortar molding machine integrates mold carrying, automatic stirring and pouring, vibration molding, strickling and cleaning, and unmanned operation of the whole cement mortar molding process is achieved through collaborative operation of the carrying unit, the stirring and pouring unit, the vibration unit and the strickling unit; and through equipment height difference layout and process interpenetration, multi-batch molding parallel operation can be realized, the molding period is greatly shortened, and the laboratory detection flux is improved.

The invention discloses a universal molecular beacon kit for various animal-derived components, and belongs to the technical field of molecular biological detection. Aiming at gene segments with specific structural characteristics in 11 common livestock and poultry animal genomes, the invention designs a composition of a universal primer and a molecular beacon fluorescent probe. The combination can be used for carrying out real-time fluorescent PCR detection and melting curve analysis by using the kit and the method by taking genome DNA of a sample to be detected as a template. By comparing the shape of a melting peak of a detected sample and the characteristic melting temperature (Tm value) of the detected sample, 11 animal-derived components of livestock and poultry can be quickly and accurately identified under the condition of single-tube detection. Technical innovation is achieved in the field of molecular biological detection, an efficient and reliable solution is provided for animal-derived component detection, and the method has important practical value and popularization potential.

The invention discloses a Dean flow sorting device and method for on-line quantitative detection of cow's milk somatic cells, and belongs to the technical field of microfluidic cell detection. The device comprises a real-time cow milk sample collection unit, a sample inlet throttle valve, a Den flow micro-fluidic chip, a somatic cell enrichment milk sample outlet throttle valve, a somatic cell deficiency milk sample outlet throttle valve, an enrichment sample impedance detection unit, a deficiency sample impedance detection unit, a data processing unit and an online display unit. Interference suppression is realized by utilizing a sorting function of a Den flow micro-fluidic chip, somatic cells in cow milk are separated from other components, interference of complex conditions such as ion components in cow milk, environment temperature and the like on impedance data is effectively eliminated through comparative detection of somatic cell enrichment samples and somatic cell deletion samples, and the accuracy of a detection result is ensured.

The utility model relates to radio frequency circuit test technical field, more particularly to a kind of modular radio frequency detection equipment, comprising: host computer, host computer is provided with main control unit and addressing routing circuit in it;At least one slave, slave and the addressing routing circuit of host computer are connected through first communication bus;Host computer, host computer and the addressing routing circuit of host computer are connected through second communication bus;Wherein, addressing routing circuit includes: first data port, is connected to second communication bus;Second data port, is connected to first communication bus;And third data port, is connected to main control unit;Addressing routing circuit is embedded with the gate circuit for the address information in the data packet input according to first data port, and the physical data transmission path between first, second and third data port is conducted through.This utility model is through the configuration switching and parallel communication structure of hardware, realizes quick change type and high-throughput parallel detection.

The application discloses a glass insulator hidden damage nondestructive testing device based on infrared thermal wave technology, which comprises a machine box, an electric control box fixed on the outer wall of one side of the machine box, and a rotary table machine electrically connected with the electric control box and controlled by the electric control box; at least four jointed slave molds are distributed on the rotary table machine in a ring shape at equal intervals, and each jointed slave mold is used for bearing one glass insulator to be detected; a position detection assembly is installed at the top end of the machine box and electrically connected with the electric control box, and is used for detecting whether each jointed slave mold reaches a predetermined detection station. The application constructs a parallel station layout through the plurality of jointed slave molds on the rotary table machine, fundamentally changes the traditional mode that detection and auxiliary operation are performed in series, sets a reasonable step angle, covers all areas in the entire circumferential direction of the insulator, and effectively avoids missed detection caused by defects located on the back or the edge of the field of view during fixed posture detection.

The invention relates to biological detection, in particular to a microfluidic chip and an operation method and application thereof. The microfluidic chip includes: a lower housing including one or more microwell arrays formed on an inner surface of the lower housing; the upper shell comprises one or more reaction tanks which are formed on the inner surface of the upper shell and correspond to the corresponding micropore arrays, one or more waste liquid tanks which are formed on the inner surface of the upper shell and are communicated with the corresponding reaction tanks, and one or more sample injection holes which penetrate through the upper shell and are communicated with the corresponding reaction tanks; the air holes penetrate through the upper shell and are communicated with the corresponding waste liquid pools; wherein the inner surface of the lower shell and the inner surface of the upper shell are opposite to each other and are in sealing fit with each other, so that one or more independent reaction units are formed. The device has the advantages of high detection flux, wide dynamic range, anti-pollution function, reliable, sensitive and rapid detection, simple and compact structure and low manufacturing cost.

The application provides a STRC gene copy number variation detection method based on whole genome sequencing. The STRC gene and the STRCP1 gene are subjected to sequence alignment to find each difference site of the STRC gene and the STRCP1 gene. For each difference site, the sequence of the corresponding STRC position and STRCP1 position in the genome is read from a variation detection file. The total copy number of the true gene and the false gene is calculated by taking the reference site in the genome as a reference. The STRC gene copy proportion on each difference site is calculated. The STRC gene copy number on each difference site is calculated according to the total copy number and the STRC gene copy proportion. The STRC gene copy number on each exon is determined according to the STRC gene copy number on each difference site. The method can realize the detection of the STRC copy number, simplifies the detection process, improves the detection throughput and reduces the cost.

The utility model provides a soil organic matter analysis device capable of processing samples in batches, which belongs to the technical field of soil analysis and comprises a bearing mechanism, a working box, a driving mechanism arranged at the top of the working box and a cleaning component arranged at the bottom of the driving mechanism. According to the utility model, the multi-station turntable is matched with the eight groups of annularly arranged bearing tables to realize batch sample circulating treatment, so that the detection flux is obviously improved; a fan and an air blowing pipe head of the soot blowing assembly are combined with a rolling brush cylinder of the sweeping assembly to form a dual cleaning system, and viscous substances and cakes on the surface of the sample are effectively removed; a detection probe of the analysis assembly realizes multi-point automatic detection through a linear guide rail; a guide plate and a collecting box of the collecting mechanism clean residues in time, and dirt accumulation is avoided; the problem that traditional single sample detection efficiency is low is solved, automatic batch processing is achieved, meanwhile, equipment blockage is effectively prevented through multiple cleaning and a waste collecting system, the detection efficiency is remarkably improved, and the service life of the equipment is remarkably prolonged.

The utility model provides a sample analyzer which comprises a tip gun head consumable mechanism, a sample bin, a reagent bin, a reaction cup feeding bin, an incubation mechanism, a mixing mechanism, a magnetic separation mechanism, a gripper mechanism and a detection mechanism outside the base, the reaction cup feeding bin comprises a reaction cup arranging mechanism and a reaction cup temporary storage mechanism, the uniform mixing mechanism comprises a first uniform mixing station and a second uniform mixing station, the gripper mechanism comprises a first gripper mechanism and a second gripper mechanism, and the first gripper mechanism and the second gripper mechanism are located in different planes. All functional modules of the sample analyzer are reasonably arranged, the size is reduced, different scheduling processes can be met, the number of reaction cup containers on the input and output uniform mixing mechanism in unit time and the transfer efficiency of the reaction cup containers are improved, and finally the detection flux is improved.

The present invention relates to the field of biotechnology, and specifically to SSR marker detection primers and uses for potato homologous chromosome typing identification, which can identify homologous chromosomes of chromosome IV, chromosome V, chromosome VII and chromosome XII in "Cooperation 88", and can determine the ploidy of the test sample by combining the homologous chromosome typing results with other ploidy detection methods. Compared with chromosome karyotype analysis, chromosome banding technology and fluorescent in situ hybridization technology, the detection throughput is high, and the detection results are more objective, more accurate and reproducible. Compared with genome sequencing technology, no special dedicated instruments and equipment are required, the detection cost is lower, the time consumption is shorter, and the operation is simpler; homologous chromosome typing detection using PCR amplification results has basically no special requirements for detection materials, and compared with whole genome sequencing, experimental skills and related data processing capabilities are relatively much lower.

The application discloses a micro-fluidic chip integrating nucleic acid extraction and high-throughput ECL detection, and a preparation method and application thereof. The micro-fluidic chip comprises a cover sheet, an intermediate layer and a substrate from top to bottom. The intermediate layer is provided with a nucleic acid extraction reagent storage bin, an electrochemical luminescence reagent storage bin, an electrochemical luminescence reaction bin and a waste liquid bin. The substrate is provided with a gold electrode corresponding to the electrochemical luminescence reaction bin. The preparation method comprises the following steps: substrate cleaning, nano-gold preparation, substrate gold electrode preparation, PDMS glue preparation and micro-fluidic chip construction. The application has the advantages that the operation process of nucleic acid extraction reduces external pollution, improves nucleic acid detection throughput, shortens nucleic acid extraction and detection time, reduces detection cost, and greatly reduces professional requirements for nucleic acid extraction and detection.

The present invention discloses an integrated microfluidic chip for capturing and incubating circulating tumor cells, belonging to the field of microfluidic technology. The integrated microfluidic chip of the present invention comprises an upper chip, a middle chip and a bottom chip connected in sequence from top to bottom. The first microcolumn array in the upper chip realizes the primary capture of circulating tumor cells. The drainage channel arranged in the middle chip is used to introduce the blood sample from the upper chip into the center of the bottom chip, so that the blood sample flows radially into the U-shaped microcolumn unit for secondary capture. The radially distributed U-shaped microcolumn units make the fluid flux very large, the blood sample flow rate is fast, and the capture rate is high. The present invention overcomes the shortcomings of the prior art microfluidic chips with low flux, insufficient number and purity of enriched tumor cells, resulting in inaccurate detection process. The integrated microfluidic chip provided integrates the capture function and the incubation function, and has high throughput and high capture rate.

The present invention provides a carbon nanotube transistor flexible ion sensor array, comprising a flexible substrate and an ion sensitive array area and a transistor signal amplification area formed on the flexible substrate and connected by metal leads. The ion sensitive array area adopts a multi-sensitive electrode parallel structure with a common reference; the transistor signal amplification area includes a semiconductor single-walled carbon nanotube network film channel, a metal source and drain electrode, a high-K gate dielectric layer, and a metal gate electrode. The sensor array of the present invention adopts a structural form in which the sensitive area is separated from the channel interface, which gives full play to the intrinsic characteristics of the high carrier mobility of carbon nanotubes, improves the sensitivity of the device, and effectively improves its stability, accuracy and repeatability. The present invention also provides a method for preparing a carbon nanotube transistor flexible ion sensor array, which has the characteristics of mild process conditions, simple steps, and compatibility with flexible substrates.