647results about How to "High detection throughput" patented technology

The invention provides a chemiluminescence immunoassay device which comprises a sample supply mechanism, a chemical reaction incubation mechanism, a sample adding mechanism and a luminescence detection mechanism, wherein the sample supply mechanism comprises a plurality of sample tubes, a sample disc, a plurality of reagent tubes and a reagent disc, wherein the sample disc and the reagent disc are respectively in a circular ring shape and can rotate. The chemical reaction incubation mechanism is set to be at constant temperature, and comprises a rotary table, a plurality of reaction discs, a plurality of reaction cups and a driving mechanism, wherein the rotary table is in a circular shape; the plurality of reaction discs are arranged on the rotary table at equal intervals along the circumferential direction of the rotary table; reaction cup containing cavities are respectively arranged along the circumferential directions of the reaction discs at equal intervals; the plurality of reaction cups are respectively arranged along the circumferential directions of the reaction discs at equal intervals; the driving mechanism is connected with the rotary table and the plurality of reaction discs by power, so that the rotary table can rotate, and the reaction discs are driven to make revolution or respectively rotate. The sample adding mechanism comprises a liner movement unit, a sample needle, a reagent needle and a cleaning groove. The luminescence detection mechanism comprises a cleaning needle, a substrate needle, a light insulation cover and a detection head.

The invention relates to a quantitative detection device based on fibrous-membrane gathering and separation and a detection method thereof. The quantitative detection device comprises a separation testing cup, a reaction cup, an analysis buffer solution and a cleaning solution; wherein the separation testing cup comprises a receiving tank, a water absorption cup and millipore filters between them. A capture agent and a labeling agent are solidified on the bottom of the reaction cup and the capture agent is fixed on the surface of latex beads. The detection method comprises: conjugation reaction, separation, cleaning and detection. In the invention, not only the characteristics of convenient operation and rapid detection of immune infiltration (IF) and immunochromatography (IC) are maintained, but also precision and sensitivity of the detection are obviously better than that of IF and IC. Therefore, quantitative immunodetection and nucleic acid hybridization analysis can be implemented effectively. The invention can be used for the quantitative detection of substances such as protein, nucleic acid, small molecule and microbe.

The invention provides an indirect competitive quantum dot fluorescence immunological detection method for synchronously detecting multiple small molecular compounds and detection kit, wherein the immunological detection method is a liquid phase immunological detection method which uses an encoded microsphere as a solid phase carrier and a quantum dot as a fluorescent marker and is used for competitive specificity reactions of a small molecular compound antigen. c Firstly, a captured antigen is covalently bound to the surface of the encoded microsphere, an indirect competitive fluorescent immune complex of microsphere-captured antigen-detected antibody-second antibody-quantum dot is formed in a filter film plate reaction hole through capturing the antigen, detecting the antibody and binding the second antibody specificity, and then the indirect competitive fluorescent immune complex flows across a suspension chip or a detection region of a flow analysis system one by one under the restraint of the sheath fluid, recognizes different encoded microspheres and detects the fluorescent intensity (or average fluorescence intensity) of the quantum dot to complete the detection. A plurality of small molecule compounds in the same sample can be synchronously detected, and one or more small molecule compounds in different samples can also be detected. The invention has the advantages of fast speed and high flux.

The invention belongs to the technical field of food quality and safety detection and particularly relates to a method for rapidly detecting pork, beef, mutton and chicken components in food at the same time through animal genomic DNA (deoxyribonucleic acid) extraction, primer design and UP-M-PCR (universal primers-multiplex-PCR). The four meat components can be distinguished rapidly at the same time according to differential sites of mitochondrial cytochrome b of the animal by using the forward primer sharing and reverse primer specificity strategy and an M-PCR system including five primers. The result shows that the method has the advantages that the detection limit can be up to the pictogram level, the components of the reaction system are free from cross interference and the meat products on market can be detected, verified and distinguished accurately by sampling 80 meat products randomly according to the specificity of the four target meat components. In a word, the invention provides a specific, sensitive and practical multiplex PCR method for rapidly screening four common meat components in food at the same time.

Disclosed are a method for analyzing high-throughput nucleic acid and an application thereof. Among n nucleic acid fragments to be analyzed, for each target nucleic acid fragment, at least two specific probes bound to different binding domains of the target nucleic acid fragment are provided, each specific probe has a specific binding domain and a universal sequence domain, a sequence of the specific binding domain and a sequence of the binding domain of the target nucleic acid fragment are complementary, and a sequence of the universal sequence domain is corresponding to a sequence of a sequencing primer.

The patent of the invention discloses a multi-parameter oil detecting device and a manufacturing method thereof. The detecting device is used for distinguished detecting ferromagnetic metal particles,non-ferromagnetic metal particles, water drops and bubbles in oil. Based on a manufacturing method of a micro-fluidic chip, the dead center of a micro-channel is filled with a metal rod for the firsttime, and a capacitance detection mode is introduced; a ring runner is arranged in the micro-fluidic chip for the first time, an inner hole of a planar inductance coil is sufficiently used, metal particles are enabled to pass through a most sensitive detection area, and the detection flux of the chip is improved when the detection precision is ensured; and a vertical runner is adopted to reduce the adhesion possibility of pollutants in oil on the wall of the runner, and the deposition and blockage of the pollutants in the runner are effectively prevented. The chip can complete the distinguished detection of particulate pollutants in lubricating oil and hydraulic oil, a new method is provided for comprehensive rapid detection of machines and devices on oil, and fault diagnosis can be performed on the machines and the devices.

The invention provides a method for fast detection of residual amount of restricted organic solvents in a textile. An extraction agent is utilized for extraction, and an extraction solution is determined by using gas chromatography-mass spectrometry. Compared with the prior art, a conventional chemical analytical instrument of gas chromatography-mass spectrometry is adopted for detecting the content of a variety of harmful organic solvents in the textile, the pretreatment is fast and simple, detection limits are low, the accuracy is high, and the detection throughput is high, so that the method is very suitable for batch detection of restricted substances; and in addition, by adopting the detection method provided by the invention, the current blank in the method for detecting the content of the variety of the harmful organic solvents in a light and textile product is filled up, important technical support is provided for product quality control and safety monitoring, and the method can be used for detecting the residual amount of the organic solvents in textile products, leather products and various raw materials and further has good economic and social benefits.

The invention provides a method and a kit for detecting a drug resistance gene mutant type of tuberculous bacillus (TB). Compared with the prior art, the invention has the following advantages: 1) reagents and consumables used in the invention are simple and stable, and expensive reagents like fluorescent dyes and special enzyme are not needed; 2) a reaction can be carried out in a microscale system, and usage of a sample and a variety of consumables is reduced; 3) since mass spectrometry is used for direct detection of molecular weight (a mass-to-charge ratio) of DNA and direct determination of types of bases (i.e., no need for signal conversion in any manner), it is theoretically practicable that identification can be realized as long as one copied amplified DNA fragment is amplified, so high sensitivity is obtained; and 4) since mass spectrometry also has the characteristics of automatic and high-flux detection and the like, combination of mass spectrometry and multi-primer extension enables a plurality of drug resistance sites to be simultaneously detected in one reaction system, thereby substantially reducing workload and increasing detection flux.

The invention provides a method for detecting the HPV genotypes, with the gene to be detected being one or several types of the type 17 HPV, comprising the following steps: according to the variant sites of the selected HPV genotype universal primer sequence to be detected, an amplification primer aiming at each type is designed; the specific extension primer of each type is designed; (2) PCR amplification is conducted; (3) SAP enzyme treatment is conducted; (4) extension reaction is conducted, among the extending products and the extending primers, the difference of the molecular weight among the extending products of each type is not less than 9D; (5) resin is used to purify extension reaction products; and (6) mass spectrometry detection is conducted, and the type of HPV gene to be detected is determined. By using the method, the invention solves the problems of some of existing detection methods that the typing can not be realized, the multiple infections can not be detected, the accuracy is limited, the throughput is low, the cost is high, and the stability of reaction may be affected as the probe is RNA.

The invention discloses a genome simplification and next-generation sequencing-based deoxyribose nucleic acid (DNA) library preparation method and a kit in the field of biotechnologies. The method and the kit aim to overcome the defects of the conventional library preparation method and can be used for whole genome single nucleotide polymorphism (SNP) detection and genetic typing of species of which the reference genomes are not perfect and the genealogies of research groups are not clear, and which do not have haplotype maps. The DNA library preparation method and the kit are simple in operation process, the prepared library is high in sequencing quality, the segment distribution among individuals is low in variability, the research cost is low, and the DNA library preparation method and the kit have very wide application prospect in the aspect of realizing the high-throughout whole-genome SNP detection and genetic typing research.

The invention discloses a non-small cell lung cancer targeted therapy gene detection method, and belongs to the field of gene detection. The method for detecting 466 mutations of 12 oncogenes is developed by multiplex PCR and high throughput sequencing technologies, wherein the oncogenes are AKT1, ALK, BRAF, EGFR, ERBB4, FGFR1, FGFR2, FGFR3 , KRAS, MET, PIK3CA, and PTEN, and the mutations may be substitutions, insertions and / or deletions of one or more bases. The detection method provided by the invention has the advantages of high detection sensitivity of up to 0.01%, and clear and objective detection results, can be directly used for reflecting specific mutation sites of the relevant gene, directly used for guiding clinical non-small cell lung cancer targeted dosage, and used for early diagnosis or auxiliary diagnosis and screening of cancers as well as post-cancer surveillance.

The invention discloses a primer, a kit and a method for determining a lung cancer gene mutation site based on a high-flux sequencing technology, which belongs to the field of biological molecular detection. The invention discloses a method and a kit for determining a lung cancer gene mutation site based on a high-flux sequencing technology. The method comprises the following steps: extracting tumor tissue DNA; designing a panel of a lung cancer targeting treatment molecular diagnosis associated gene; performing the PCR primer amplification; and establishing a library, and performing the high-flux sequencing. The specific primer and the kit for determining the lung cancer gene mutation site are used for detecting 316 mutation situations of 16 oncogenes, and the mutation can be the replacement, insertion and/or deletion of one or more alkaline groups. The sensitivity of the detection method and the kit of the invention can reach up to 1 percent, the detection result is definite and objective and can directly reflect the specific mutation site of the reaction associated gene and has important significance for early diagnosis or auxiliary diagnosis and screening of the cancer and theprognosis monitoring of the cancer.

The invention provides a construction method for a simplified genome next generation sequencing library based on double enzyme digestion and a kit. Aiming at defects of an existing construction method for the double enzyme digestion simplified genome next generation sequencing library, the double enzyme digestion combined range is expanded, and excessive dependence on expensive instruments of constructing the simplified genome library is reduced, the library construction flow path is simplified, library construction cost is reduced, the sequencing efficiency is improved, and meanwhile the technology is easy and flexible to operate and easier for researchers to master and can be realized in a common molecule lab. The construction method is particularly suitable for miniature or medium-scale labs needing to conduct SNP molecular marker development, genetic map construction, population genetics research, phylogeny biological research and the like on a great number of species with incomplete reference genomes. The construction method has good practical application value and application prospects in the fields of molecular breeding of agriculture, conservation biology and evolutionary biology.

The invention discloses a multiple PCR detection kit for 11 intestinal pathogen nucleic acid and application of the detection kit. The detection kit comprises primers for amplifying 11 intestinal pathogens which include vibrio cholerae serotype O1, vibrio cholerae serotype O139, salmonella, Shigella, vibrio parahaemolyticus, Yersinia enterocolitica, enterophathogenic escherichia coli (EPEC), enteroinvasive escherichia coli (EIEC), enteroaggregative escherichia coli (EAEC), enterotoxigenic escherichia coli (ETEC) and escherichia coli O157:H7. The multiple PCR detection kit has the advantages that the kit is capable of performing multiple detection, high in sensitivity and fast and convenient to use; specific primer sequences are used to guarantee detecting result reliability; the detection method is simple to operate, time saving, labor saving, high in detection throughput, low in reagent consumable cost, capable of directly detecting the nucleic acid extracted from encephalitis pathogens, low in detection platform and staff technical level requirements and capable of being widely popularized in conventional detection.

The invention discloses a multiplex nucleic acid visualization detection method and kit based on solid phase rolling circle amplification and particle aggregation. The provided method utilizes the technologies of solid phase rolling circle amplification on a sheet and magnetic particle aggregation to achieve the visualization detection on multiplex nucleic acid characteristic sequences of a sample. The provided method has the characteristics of high detection flux, high sensitivity, quick detection speed, and simple operation. Furthermore, the method does not need expensive thermal amplification cycler or fluorescence detection system, can be used in medical service platforms in different levels, and are especially suitable for different medical departments in remote areas or developing areas.

The invention provides a method, oligonucleotide and kit for detecting common high-risk HPV (human papilloma viruses). A fluorescence PCR (polymerase chain reaction) technology is adopted, HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 26, 53, 66, 73 and 82 which possibly exist in a sample are subjected to initial detection and type identification. By the method, oligonucleotide and kit for detecting the common high-risk HPV, 18 high-risk types can be detected simultaneously, and the HPV 16 and 18 can be subjected to genetic typing simultaneously. On the basis of 18 types of high-risk HPV and affinity of other types on a phylogenetic tree, a high-risk HPV primer and a probe are designed, a background fluorescence value is reduced remarkably while detection accuracy, sensitivity and specificity are guaranteed, detection flux is increased remarkably, and detection cost is greatly reduced.

The invention relates to a method for simultaneously detecting five kinds of steroid hormones in serum. The five kinds of steroid hormones are respectively testosterone, androstenedione, 11-deoxycortisol, cortisol and cortisone. The method for simultaneously detecting the five kinds of steroid hormones in the serum comprises the following steps: sample pretreatment: adding an acetonitrile solution of an internal standard substance in a serum sample for carrying out protein precipitation, then adding tert-butyl methyl ether for extracting, blow-drying supernatant, adding a combination solution, and taking the supernatant, thus obtaining a to-be-detected sample; enrichment, separation and detection: carrying out enrichment, separation and detection on the to-be-detected sample by adopting a two-dimensional liquid chromatography-tandem quadrupole mass spectrometer. According to the method for simultaneously detecting the five kinds of steroid hormones in the serum, disclosed by the invention, the method is adopted for simultaneously detecting five kinds of compounds of which the concentration ranges are inconsistent in the serum, and the time for simultaneously detecting the five kinds of the compounds is about 8.5 to 12.0 minutes; the method for simultaneously detecting the five kinds of steroid hormones in the serum is low in cost, high in flux, high in precision degree and strong in specificity.

The invention discloses a deafness susceptibility gene mutation detection kit as well as a preparation method and application thereof, and relates to gene mutation detection. The kit comprises a kit body, a cover, a partition, an HHL (Hereditary Hearing Loss) PCR (Polymerase Chain Reaction) mixed liquor bottle A, an HHL PCR mixed liquor bottle B, an HHL PCR mixed liquor bottle C, an HHL PCR mixed liquor bottle D, an HHL enzyme mixed liquor bottle, an HHL standard control bottle, and an HHL negative control bottle. The preparation method comprises the following steps: preparing an amplification reagent and a contrast reagent firstly, arranging the amplification reagent and the contrast reagent in the bottles and finally, obtaining the deafness susceptibility gene mutation detection kit. The kit can be applied to screening of hereditary deafness genes and identification of deafness causing genes, so as to prevent deafness.

The invention provides an alpha-globin gene mutation detection kit as well as a preparation method and a use thereof, and relates to protein gene mutation detection. The alpha-globin gene mutation detection kit is provided with a box, an amplification reagent bottle and a control reagent bottle; the amplification reagent bottle and the control reagent bottle are arranged in the box. The preparation method comprises the following steps: 1) preparing an amplification reagent which comprises an HBA PCR mixed solution and an HBA enzyme mixed solution; 2) preparing a control reagent which comprises HBA standard control and HBA negative control; and 3) putting the amplification reagent prepared in the step 1) and the control reagent prepared in the step 2) in the box, thereby obtaining the alpha-globin gene mutation detection kit. The alpha-globin gene mutation detection kit can be applied to detecting the alpha-globin gene mutation. The alpha-globin gene mutation detection kit is convenient and fast, capable of detecting a plurality of sites by use of one single reaction, short in time consumption, high in detection flux, high in detection specificity, and easy in result interpretation.

The invention provides a method and a kit for detecting individualized medication related genes in tumor chemotherapy. Compared with the prior art, the method and the kit have the following advantages: 1) the reagent consumables are simple and stable, and expensive reagents, such as fluorescent dyes, special enzymes and the like, are avoided; 2) the reaction can be carried in a micro scale system, thus reducing usage of the sample and the consumables; 3) theoretically, the genes can be recognized as long as one copied amplified DNA fragment is amplified since mass spectrum technology can directly detect the molecular weight (mass-to-charge ratio) of DNA and can directly determine the types of basic groups (i.e., signal conversion in any form is unnecessary), so that the method has high sensitivity; and 4) the mass spectrum technology also is automatic and allows high-throughput detection, so that when the mass spectrum is combined with multi-primer extension technology, several gene loci can be detected in the same reaction system, thereby greatly reducing work load and increasing detection throughput.

The invention relates to a detection chip, a detection kit and a detection method for bioterrorism factors. Probe molecules and PCR amplification primers are designed according to the gene conservation regions of various bioterrorism factors, and multiple probe molecules are integrated into one detection method. On the chip, the detection throughput is high. At the same time, due to the application of gene chip detection, the detection sensitivity is high and the speed is fast; according to the analysis of the designed PCR amplification primer sequence, multiple PCR technology can be used to detect nucleic acid substances of various bioterrorism factors at one time. Amplification, thereby further speeding up the detection speed.

The invention relates to the field of microbiological detection, in particular to a method and a device for microbiological analysis of a host sample. The method comprises the following steps: (1) performing first filtering processing on a sequencing data set from a host sample by adopting a host genome database so as to remove sequencing data which can be compared with the host genome database from the sequencing data set; (2) performing second filtering processing on the sequencing data set by adopting a homologous database so as to remove the sequencing data which can be compared with the homologous database from the sequencing data set; and (3) comparing the sequencing data set subjected to the first filtering treatment and the second filtering treatment with a microbial genome database so as to determine microbial sequencing data from the microorganisms in the sequencing data set. By applying the method and the device provided by the invention, rapid and accurate analysis and detection of microorganisms in a host can be realized.