11results about How to "High detection throughput" patented technology

The utility model relates to radio frequency circuit test technical field, more particularly to a kind of modular radio frequency detection equipment, comprising: host computer, host computer is provided with main control unit and addressing routing circuit in it;At least one slave, slave and the addressing routing circuit of host computer are connected through first communication bus;Host computer, host computer and the addressing routing circuit of host computer are connected through second communication bus;Wherein, addressing routing circuit includes: first data port, is connected to second communication bus;Second data port, is connected to first communication bus;And third data port, is connected to main control unit;Addressing routing circuit is embedded with the gate circuit for the address information in the data packet input according to first data port, and the physical data transmission path between first, second and third data port is conducted through.This utility model is through the configuration switching and parallel communication structure of hardware, realizes quick change type and high-throughput parallel detection.

The application provides a STRC gene copy number variation detection method based on whole genome sequencing. The STRC gene and the STRCP1 gene are subjected to sequence alignment to find each difference site of the STRC gene and the STRCP1 gene. For each difference site, the sequence of the corresponding STRC position and STRCP1 position in the genome is read from a variation detection file. The total copy number of the true gene and the false gene is calculated by taking the reference site in the genome as a reference. The STRC gene copy proportion on each difference site is calculated. The STRC gene copy number on each difference site is calculated according to the total copy number and the STRC gene copy proportion. The STRC gene copy number on each exon is determined according to the STRC gene copy number on each difference site. The method can realize the detection of the STRC copy number, simplifies the detection process, improves the detection throughput and reduces the cost.

The invention belongs to the technical field of molecular biology, and discloses a molecular marker combination for identifying wax gourd germplasm resources, the molecular marker combination comprises 48 SNP molecular markers, the physical positions of the 48 SNP molecular markers are determined by sequence alignment based on a wax gourd reference genome GCF009727055.1, and the chromosome positions and base variation of the 48 SNP molecular markers are shown in the specification table 1. The invention further discloses a primer group for detecting the molecular marker combination, the primer group comprises 48 pairs of primers, and the sequence of each pair of primers is shown in the specification table 2. The invention also discloses a kit which comprises the primer group. The invention also discloses applications of the molecular marker combination, the primer group and the kit. According to the molecular marker combination for identifying the wax gourd germplasm resources, 48 SNP markers are screened, and the 48 molecular markers are high in typing quality, single in copy and high in polymorphism.

This invention relates to a chip for identifying polysaccharides from traditional Chinese medicine containing the structure →4)-β-D-Manp-(1→ and its application, belonging to the field of biochip technology. The chip of this invention comprises a solid-phase support and a microarray system, wherein the microarray system is fixed on the surface of the solid-phase support. Using a glass substrate treated with hydrazide as the carrier, the invention precisely spots the target polysaccharide, polysaccharide standards, and negative / positive controls using a spotting instrument to construct a polysaccharide chip containing 16 subarrays. After blocking the non-specific binding sites of the chip with a blocking buffer, fluorescently labeled LM21 antibody is added to the subarrays for incubation. By collecting fluorescence signals, the relative fluorescence unit value is obtained, directly reflecting the binding strength between the polysaccharide and the LM21 antibody. This invention is simple to operate, has high detection sensitivity, and strong structural recognition specificity, and can be widely used for the rapid screening and precise structural characterization of active polysaccharides from traditional Chinese medicine, providing an efficient technical means for the development and utilization of polysaccharides from traditional Chinese medicine.

This invention discloses a KASP molecular marker related to the thickness of soybean main stem and its application, belonging to the field of molecular genetics and breeding technology. The nucleotide sequence of the KASP molecular marker is shown in SEQ ID NO.1, and a T / C base mutation exists at position 26 of the sequence shown in SEQ ID NO.1. Compared with traditional markers, the development of the KASP molecular marker of this invention significantly improves the detection throughput, allowing a large number of samples to be processed in a single reaction, greatly improving the efficiency of genotyping and breeding selection; it can accurately distinguish SNP variations, and the genotyping results are accurate and reliable.

The application discloses a molecular marker primer for a narrow cod and application thereof, and relates to the technical field of molecular biology markers. The primer combination is composed of three pairs of primers, namely, GC6:TC14, GC8:GT15 and GC9:TG9, and the sequences of the primers are shown as SEQ ID NO. 1-6. The application develops, for the first time, the STR molecular marker primer combination specially used for sea area source identification of the narrow cod. Compared with the microsatellite marker used only for the population genetics basic research of the narrow cod in the prior art, the primer combination is optimized through multi-stage screening, can effectively distinguish the narrow cod samples from the Okhotsk Sea FAO 61 sea area and the Bering Sea FAO 67 sea area, and fills the technical blank in the sea area tracing field of the cross-border trade of the narrow cod.

The invention provides an anti-stem cell antibody detection method and a kit. According to the method provided by the invention, the anti-stem cell antibody can be detected by adopting an MSD platform, and magnetic beads can be used for replacing stem cells to serve as an immunosuppression competition confirmation reagent to carry out a confirmation experiment. The detection method based on the MSD platform provided by the invention can improve the sensitivity of the method for detecting the stem cell antibody and / or improve the detection flux.

This invention discloses a SNP molecular marker combination for breeding high-expansion-multiplicity popcorn and its application, belonging to the field of molecular breeding technology. The SNP molecular marker combination of this invention consists of 30 high-effect SNP loci located on the maize B73 reference genome v4 version. This combination was selected by performing genome-wide association analysis on 399 natural popcorn accessions and ranking the top 30 loci according to their SNP effect values. This invention allows for accurate prediction of the expansion-multiplicity trait by detecting the genotypes of these 30 SNP markers in the early stages of breeding, with a prediction accuracy of over 0.72. It has significant advantages such as high selection efficiency, short cycle, low cost, and insensitivity to environmental influences, and is suitable for molecular marker-assisted breeding and multi-trait aggregation breeding of popcorn.

The application discloses a kind of multi-channel integrated capillary electrophoresis chip and flow path control method, belong to detection chip technical field.It includes: chip ontology, which is provided with sample inlet through hole, separation channel, plunger valve port, plunger valve channel;Sample inlet through hole is communicated with separation channel and is used for sample introduction;Separation channel is provided with multiple, any one separation channel is configured with a sample inlet through hole, plunger valve channel is communicated with separation channel by plunger valve port;Separation channel includes straight line section, first curve extension section and second curve extension section, and the total length of the straight line section, the first curve extension section and the second curve extension section of each separation channel is the same.Through integrating multiple separation channels with equal total length, each channel is equipped with independent sample inlet through hole and detection window, multi-channel synchronous detection is realized, and the unit length electric field intensity of each separation channel is consistent, effectively eliminates migration time drift, and improves multi-channel detection consistency.

The utility model discloses a kind of egg spectrum acquisition device based on pre-scanning, further include optical breadboard, optical breadboard is equipped with pre-position mechanism, the both sides of pre-position mechanism are provided with light source and dark box pillar, the upper end of dark box pillar is equipped with dark box, and the inside of dark box pillar is provided with spectrometer mechanism, the lower end of dark box is equipped with several light holes, light hole is used to place egg, and the inside wall of dark box is equipped with lens a and photoelectric detector, lens a is located between egg and photoelectric detector. By setting pre-position mechanism and spectrometer mechanism before light irradiation to egg, make light before entering egg carries out spectrometer, replace after egg emission and then carry out spectrometer, so as to replace spectrum camera by low-cost photoelectric detector and optical filter, the data acquisition card of acquisition end can directly collect corresponding wavelength light, and also can be expanded detection array by adding lens and photoelectric detector, so as to improve detection flux.

This invention discloses a semi-quantitative RT-LAMP method and kit for viral nucleic acid detection based on dispensed lyophilized microspheres and a multi-channel microfluidic chip. The method includes: designing two independent LAMP primer sets (P1 and P2) for each of the SARS-CoV-2 Omicron strain N gene, Zika virus NS5 gene, H1N1 influenza virus M1 gene, and H3N2 influenza virus NP gene; preparing two independent types of lyophilized microspheres by independently mixing RT-LAMP premix containing Bst DNA polymerase and other components with LAMP primer mixture and a lyophilization protectant; and using an N-channel microfluidic chip to perform serial dilutions and parallel RT-LAMP amplification of the same sample, determining the concentration of viral nucleic acid in the sample by the positive / negative inflection points of each channel. This invention is simple to operate, requires no equipment such as a real-time PCR instrument, and is suitable for rapid virus detection in various scenarios.