392 results about "Capillary electrophoresis" patented technology

The present invention provides the method for the separation of at least more than two Low molecular weight (LMWs) impurities present in the protein mixture comprising fusion protein CTL4-IgG1 by using Capillary Electrophoresis-Sodium dodecyl sulfate (CE-SDS) wherein the loading amount of protein mixture is more than 270 μg. The invention further provides a ratio of protein to sodium dodecyl sulfate is 2.7:1 or higher.

The invention belongs to the field of biological detection, and particularly relates to an ewing sarcoma detection primer group, a kit and application thereof. The Ewing sarcoma detection primer group comprises a primer group A and a primer group B, and totally has 17 primer sequences. The primer group is designed, Ewing sarcoma characteristic variation genes are detected through multiple fluorescent PCR and a high-resolution capillary electrophoresis apparatus, 24 variation types fused by 7 types of genes can be detected at a time, the lowest detection limit is 10 copies, and the shortest detection time is 240 minutes. The detection result of the primer group disclosed by the invention can pass Sanger sequencing verification and is completely consistent with the morphological diagnosis result, the methodological consistency reaches 100%, and a sensitive and efficient Ewing sarcoma genetic variation detection method is provided.

The invention discloses a molecular biological method capable of simultaneously detecting duck-derived and goose-derived components and application of the molecular biological method, after genome DNA of a sample to be detected is subjected to PCR amplification and capillary electrophoresis through primers shown in SEQ ID NO.1 and SEQ ID NO.2, sequence comparison is carried out, when the peak value is 999bp, it is indicated that the sample contains the duck-derived component, and when the peak value is 992bp, it is indicated that the sample contains the goose-derived component, and when the peak value is 992bp, it is indicated that the sample contains the goose-derived component, it is indicated that the sample contains the duck-derived component. When the peak values are 999bp and 992bp, the duck-derived component and the goose-derived component are contained at the same time. The detection kit developed on the basis of the method can generate considerable economic benefits and good social values.

The invention discloses a 26 Multi-InDel genetic marker system for highly degraded sample typing, a detection primer and application of the 26 Multi-InDel genetic marker system, and belongs to the technical field of forensic medicine identification. According to the invention, through bioinformatics screening and experimental verification, a multi-InDel genetic marker system of which the lengths of 26 amplicons do not exceed 125 bp is constructed. The problem that degradation detection material detection and high system efficiency are difficult to consider at the same time is solved. The random matching probability of the system in Han population in Hunan reaches 2.25 * 10 <-17 >, the cumulative paternity exclusion rate is 0.999925, complete typing of highly degraded DNA can be achieved, triad paternity test can be completed, and a new technical scheme which is efficient and compatible with a capillary electrophoresis platform is provided for forensic practice.

The invention provides a primer / probe composition for capillary electrophoresis analysis, a detection method and application. The primer / probe composition comprises a forward primer, a reverse primer and a nucleic acid probe, the 5'end of the nucleic acid probe carries a fluorescent label, and both the forward primer and the reverse primer do not carry the fluorescent label. Different targets are identified by detecting the nucleic acid probe, so that the length of an amplification product is not limited, only the nucleic acid probe carries a fluorescence signal without generating a non-specific fluorescence signal, the generation of a supersaturated fluorescence signal can be effectively prevented by using the nucleic acid probe with a specific concentration, the fluorescence signals among different targets do not interfere with each other, and the detection sensitivity is high. And multicolor fluorescence can be used at the same time.

The invention provides a capillary electrophoresis analysis method of serum amino acid. The capillary electrophoresis analysis method comprises the following steps: S1, preparing an operation buffer solution; s2, selection and pretreatment of a quartz capillary column; s3, conditions are analyzed through electrophoresis; s4, drawing a quasi curve; s5, sample derivatization; s6, quantitative analysis. According to the method, a mixed reagent of alpha-cyclodextrin, beta-cyclodextrin and gamma-cyclodextrin is adopted for the first time, the amino acid migration behavior is cooperatively adjusted, a migration time window is expanded, the separation resolution of amino acids with similar structures is effectively improved, and the separation degree and the atlas definition are remarkably improved. According to the invention, sodium taurocholate is added, pH is regulated and controlled, a weakly alkaline buffer system with moderate conductivity and stable migration behavior is formed, separation and detection of FITC derived amino acids are facilitated, and balance of migration time distribution is optimized. The method is suitable for scenes of clinical sample analysis, disease marker screening and the like, and has good universality, stability and popularization value.

The invention relates to the field of bioinformatics and molecular biology, and discloses a gene single nucleotide polymorphism (SNP) site analysis method based on SNaPshot, which comprises the following steps: acquiring genome DNA sequence data of a sample to be detected, extracting SNP site information in the genome DNA sequence data, designing site specific primers, carrying out PCR amplification by using an SNaPshot technology, and determining the SNP site of the sample to be detected. And an electrophoretogram is obtained through capillary electrophoresis separation. By analyzing the maps, the genotype of each SNP site can be determined, and an SNP feature map can be constructed. And finally, performing statistical analysis based on the feature maps to obtain a target analysis result, evaluating gene polymorphism according to the target analysis result, and obtaining related information. According to the method, the efficiency and accuracy of SNP analysis can be improved, and support is provided for genetic research and individualized medical treatment.

The invention discloses a high-throughput composite STR (short tandem repeat) detection system based on next-generation sequencing. The high-throughput composite STR detection system comprises specific primer sequences designed for 291 STR loci; the 291 STR loci are composed of 106 autosome STR loci, one sex identification locus Amelogenin, 148 Y chromosome STR loci and 36 X chromosome STR loci, and the 291 STR loci are composed of 106 autosome STR loci, one sex identification locus Amelogenin, 148 Y chromosome STR loci and 36 X chromosome STR loci; the system is used for performing STR typing and is prepared into a kit for individual recognition, paternity identification and complex sample analysis. The STR detection system covers STR locus combinations of whole chromosomes (autosomes and X / Y chromosomes), breaks through the split design in the prior art, can overcome the limitation of the traditional capillary electrophoresis (CE) technology on complex samples, degraded samples and large-scale detection, improves the detection sensitivity, flux and accuracy, and has a good application prospect. And the requirements of identity identification of complex genetic relationships, mixed samples and disastrous events in judicial expertise are met.

The presently described and claimed disclosure relates to capillary electrophoresis methods for quantifying an intact AAV genome and protein components in an AAV using the same capillary electrophoresis system. The claimed and described approach offers an automated analysis of AAV samples and provides information to determine the AAV empty / full ratio.

The disclosure provides methods for analyzing two or more different proteins co-formulated in a sample mixture. The methods involve cation exchange-high performance liquid chromatography (CEX-HPLC), size exclusion ultra high performance liquid chromatography (SE-UHPLC), and / or capillary electrophoresis (CE) optimized to accurately determine the concentration, and assess certain critical quality attributes of, each protein in the sample.

The invention relates to the technical field of protein detection, in particular to a quantitative detection method for the content of soybean protein components. According to the quantitative detection method for the component content of the soybean protein, the capillary electrophoresis method is adopted, the detection method is optimized, efficient separation of all components of the soybean protein can be achieved, quantitative calculation can be achieved, the analysis time is short, the scheme is reliable, and reproducibility is good.

Electrophoresis device, including: a sample dish (112) on which a positive-electrode-side buffer solution container (103) containing a buffer solution and a phorese medium container (102) containing a phorese medium are arranged, and which is driven in a vertical and a horizontal direction; a thermostat oven unit (113) which holds a capillary arrangement (101) with a capillary head (403) in which a plurality of capillaries (401) are bundled in a single unit at one end thereof in a state in which the capillary head (403) protrudes downwards, and which maintains a constant internal temperature; a solution dispensing mechanism (106) for dispensing the phorese medium in the phorese medium container (102) to the capillary assembly (101) from the capillary head (403); and a current source (408) for applying a voltage to both ends of the capillary arrangement (101), wherein Openings for inserting the capillary head (403) are provided in the upper areas of the positive electrode-side buffer solution container (103) and the phorese medium container (102), and the thermostat oven unit (113) comprises a first lid element (207) which is arranged above the sample dish (112) and seals the upper part of the positive electrode-side buffer solution container (103) during the dispensing of the phorese medium by the solution dispensing mechanism (106), wherein the upper part of the positive electrode-side buffer solution container (103) is sealed by raising the sample dish (112) and pressing the positive electrode-side buffer solution container (103) against the first lid element (207).

The present invention relates to a clamp device and a detection method integrating PCR amplification and capillary electrophoresis analysis. The present invention adopts an integrated pipeline closed clamp, so the contamination source of high-concentration PCR products is effectively controlled, avoiding cross contamination between laboratory tests, and eliminating manual intervention between PCR amplification and capillary electrophoresis analysis, thereby improving test efficiency and having good application prospects.

Provided is a method and a means for improving multiplex simultaneous detection of a structural polymorphism mutation in capillary electrophoresis. In one embodiment, the present invention relates to a method for detecting a mutation in a test nucleic acid, the method including: providing a first primer set, containing at least one labeled first primer, for amplifying a nucleic acid with a first mutation; providing a second primer set, containing at least one labeled second primer, for amplifying a nucleic acid with a second mutation; amplifying the test nucleic acid with use of the first primer set and the second primer set; and subjecting an obtained amplification product to capillary electrophoresis to analyze the mutation in the test nucleic acid, the first primer and the second primer being individually labeled with combinations of different numbers and / or kinds of fluorescent dyes.

Provided is a capillary electrophoresis device in which a sample that contains a first component and a second component is injected into a capillary, the injected first component and second component are subjected to electrophoretic separation, and emission of light from the first component and the second component induced by irradiating the capillary with light is measured by a detector, whereby the signal intensity of the first component and the signal intensity of the second component are acquired, wherein: the concentration range of the first component contained in the sample includes, in addition to the concentration range in which the signal intensity of the first component is proportional to the concentration of the first component, a concentration range in which the signal intensity of the first component is lower than the saturation signal intensity of the detector and reaches the saturation signal intensity when disproportional to the concentration of the first component; and the capillary electrophoresis device quantifies the ratio of the concentration of the first component to the concentration of the second component in the sample on the basis of the ratio of the signal intensity of the first component to the signal intensity of the second component.

The invention discloses a tulip EST-SSR (expressed sequence tag-simple sequence repeat) molecular marker as well as a primer composition and application thereof. According to the invention, 15 pairs of tulip EST-SSR molecular markers and a primer composition thereof are obtained through screening, a to-be-detected tulip variety resource DNA is extracted, the 15 pairs of EST-SSR primers or synthetic fluorescent primers are respectively used for PCR amplification, a PCR product is subjected to PAGE detection or fluorescent capillary electrophoresis, and the EST-SSR fingerprint of the tulip variety resource is constructed. The method and the basis are provided for tulip variety breeding, variety authenticity identification, known variety library construction and the like.

An object of the present invention is to provide a capillary electrophoresis device and an optical power diagnostic method for the same, capable of verifying optical power without requiring any specific action. Accordingly, the capillary electrophoresis device according to the present invention comprises a capillary arrangement containing a plurality of capillaries, a light source that causes laser light to oscillate, a detection unit that detects light emitted when the capillary arrangement is irradiated with the laser light, and a control unit that performs predetermined processing based on a signal emanating from the detection unit.The control unit also extracts a predetermined absolute value with respect to an optical index based on an image captured by the detection unit and calculates the optical index by comparing the extracted absolute value with a predetermined reference value.

The invention relates to an orthogonal multichannel capillary electrophoresis gene analyzer, which is characterized in that a fluorescence excitation module is arranged to form two laser beams which are coaxially and crosswise emitted into an array capillary from two sides of the array capillary, and an optical fiber module is arranged to collect fluorescence signals emitted by all channels of the array capillary; according to the invention, fluorescence emitted from two ends of an array capillary tube deviates from an optical axis, so that aberration caused by deviation of fluorescence emitted from two ends of the array capillary tube in an objective lens collection scheme is avoided, and the number of channels of the capillary tube can be further increased, so that the period of large-scale gene sequencing is shortened; and the application range of the multichannel capillary electrophoresis gene analyzer is expanded.

The presently claimed and described technology provides methods for analyzing an encapsulated biomolecule by loading the encapsulated biomolecule on a capillary electrophoresis (CE) capillary, wherein the CE capillary is filled with a buffer comprising a polymer matrix; applying a voltage to the CE capillary to release the biomolecule from the encapsulating material; and detecting the biomolecule released from the encapsulating material. Kits for analyzing an encapsulated biomolecule are also disclosed.

Disclosed herein are methods for analyzing biological samples using capillary electrophoresis, including the characterization of genome integrity, assessment of genomic integrity and the sequencing of a nucleic acid genome, such as an RNA genome. Kits for characterizing genome integrity are also disclosed.

The application discloses a kind of molecular markers for identifying genetic sex of blue fish and its application, belong to the field of aquatic animal genetics and breeding.The molecular marker includes nucleotide sequence such as the common band of blue fish female and male fish shown in SEQ ID NO:1 and nucleotide sequence such as the unique band of blue fish male shown in SEQ ID NO:2, and the identification method comprises: taking the DNA of the blue fish sample to be identified as template for PCR amplification, after the band of PCR product is determined by agarose electrophoresis, capillary electrophoresis is carried out, if signal appears at 152bp and 168bp simultaneously, then it is male;If only signal appears at 168bp, then it is female.The molecular marker of the application can accurately obtain the genetic sex of different blue fish populations, has the advantages such as simple, fast, efficient, not affected by early undifferentiated gonad of blue fish, etc., is used for blue fish marker assisted breeding, which greatly saves the cost of aquaculture and improves the breeding efficiency.

The invention particularly relates to a capillary tube liquid cooling column oven and a capillary tube electrophoresis analyzer. The capillary tube liquid cooling column oven comprises a box body; the temperature adjusting container and the liquid storage container are arranged in the box body, a temperature adjusting device is arranged on one side of the temperature adjusting container and attached to the temperature adjusting container, and the temperature adjusting container and the liquid storage container form a first circulation loop through a circulation pump and a circulation pipeline; the liquid storage container and the peristaltic pump form a second circulation loop through thin pipes, the second circulation loop comprises a first thin pipe, a second thin pipe and a third thin pipe, one end of the first thin pipe is connected with a first liquid outlet of the liquid storage container, and one end of the third thin pipe is connected with a first liquid inlet of the liquid storage container; the other ends of the first slim tube and the third slim tube are respectively connected with two ends of the second slim tube through a tee joint; the capillary tube penetrates in from one end of the first tee joint, passes through the second thin tube and penetrates out from one end of the second tee joint. The device has the advantages that continuous and stable heat exchange cooling is provided for the capillary tube; and the temperature stability in the capillary cooling process is optimized.

The invention provides a method for detecting sulfonamides in animal-derived food through capillary electrophoresis-laser-induced fluorescence, and belongs to the technical field of detection. Comprising the following steps: (1) mincing a sample, adding an extracting solution for extraction, and carrying out solid-phase extraction column extraction and methanol elution to obtain an eluted sample solution; (2) preparing a sulfanilamide drug standard substance and fluorescamine into gradient solutions by using methanol and acetonitrile respectively, and stirring at room temperature for reaction to obtain a solution A; (3) mixing the elution sample solution with a fluorescent amine-acetonitrile solution, and stirring and reacting at room temperature to obtain a solution B; and (4) detecting the fluorescence intensity of the sulfonamides in the solution A by adopting capillary electrophoresis-laser induced fluorescence to obtain a linear equation for detecting the concentration of the sulfonamides in the solution B. The invention establishes a novel method for detecting sulfonamide residues in animal-derived food by using high-efficiency capillary electrophoresis-laser-induced fluorescence, and realizes high-sensitivity detection of three sulfonamides in animal-derived food.

The present disclosure provides methods for assessing the integrity of inverted terminal repeat sequences in AAV plasmids by restriction digestion followed by capillary electrophoresis.

A DNA analysis system is provided with: a flow channel device having a PCR chamber in which a thermal cycle is performed; and a capillary electrophoresis unit that performs electrophoretic analysis on a PCR reaction solution, in which the DNA analysis system stores values of m and n that are set in advance, thermal cycles are performed m times on the PCR reaction solution in the PCR chamber to generate a first reaction solution, a part of the first reaction solution is taken out from the PCR chamber without changing the composition, and the first reaction solution is taken out from the PCR chamber without changing the composition of the first reaction solution. Wherein, in the PCR chamber, the first reaction solution remaining in the PCR chamber is subjected to n-m (n-m is an integer of 2 or more) thermal cycles such that the total number of thermal cycles becomes n, and a second reaction solution is generated, and the first reaction solution is subjected to electrophoretic analysis in the capillary electrophoresis unit, and the first reaction solution remaining in the PCR chamber is subjected to n-m (n-m is an integer of 2 or more) thermal cycles in the PCR chamber. At least a portion of the second reaction solution is taken out from the PCR chamber without changing the composition, and the at least a portion of the second reaction solution is subjected to electrophoretic analysis in the capillary electrophoresis unit.

The utility model relates to the capillary electrophoresis appearance field, concretely relates to a high -efficient capillary electrophoresis appearance with material detection, including material cabinet, the top of material cabinet is hanked with shell, the right side of shell inner chamber bottom is hanked with electrophoresis appearance, the left side of shell inner chamber bottom is hanked with water tank, the lower portion communication of water tank left side has first water supply pipe, the upper portion of shell left side is hanked with transverse board, the top of transverse board is hanked with air cylinder. The utility model discloses through water tank, first water supply pipe, transverse board, air cylinder, water collecting tank, first water pump etc. assembly, adds the cleaning component, is convenient for automatic cleaning to the detection pipe before and after the sample, when washing, only need to draw the liquid through first water supply pipe to the water collecting tank, through the water pressure and clean water flow extrude into the sampling needle, and through the mode of spraying to the detection pipe below flush, sets up first water pump on the sample tube, and the flow size is convenient for setting, and the sample number and total amount are convenient for controlling.

To provide systems that are configured to perform multiple types of analysis, such as electrophoretic mobility and isoelectric electrophoresis within a single device, and that are automated so that multiple samples are analyzed simultaneously or in rapid succession with ease and robustness while consuming minimal volumes of expensive reagents and disposables.SOLUTION: A system includes: a housing; a cartridge retainer disposed within the housing; a detection assembly disposed within the housing; and a reagent tray holder movably disposed within the housing. The detection assembly is configured to transition between a first configuration, in which a first detector detects a first output of at least one emitter, and a second configuration, in which a second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of a capillary cartridge in fluid communication with a reagent volume.SELECTED DRAWING: Figure 6