268 results about "Capillary electrophoresis" patented technology

The invention belongs to the field of biological detection, and particularly relates to an ewing sarcoma detection primer group, a kit and application thereof. The Ewing sarcoma detection primer group comprises a primer group A and a primer group B, and totally has 17 primer sequences. The primer group is designed, Ewing sarcoma characteristic variation genes are detected through multiple fluorescent PCR and a high-resolution capillary electrophoresis apparatus, 24 variation types fused by 7 types of genes can be detected at a time, the lowest detection limit is 10 copies, and the shortest detection time is 240 minutes. The detection result of the primer group disclosed by the invention can pass Sanger sequencing verification and is completely consistent with the morphological diagnosis result, the methodological consistency reaches 100%, and a sensitive and efficient Ewing sarcoma genetic variation detection method is provided.

The invention discloses a 26 Multi-InDel genetic marker system for highly degraded sample typing, a detection primer and application of the 26 Multi-InDel genetic marker system, and belongs to the technical field of forensic medicine identification. According to the invention, through bioinformatics screening and experimental verification, a multi-InDel genetic marker system of which the lengths of 26 amplicons do not exceed 125 bp is constructed. The problem that degradation detection material detection and high system efficiency are difficult to consider at the same time is solved. The random matching probability of the system in Han population in Hunan reaches 2.25 * 10 <-17 >, the cumulative paternity exclusion rate is 0.999925, complete typing of highly degraded DNA can be achieved, triad paternity test can be completed, and a new technical scheme which is efficient and compatible with a capillary electrophoresis platform is provided for forensic practice.

The invention provides a primer / probe composition for capillary electrophoresis analysis, a detection method and application. The primer / probe composition comprises a forward primer, a reverse primer and a nucleic acid probe, the 5'end of the nucleic acid probe carries a fluorescent label, and both the forward primer and the reverse primer do not carry the fluorescent label. Different targets are identified by detecting the nucleic acid probe, so that the length of an amplification product is not limited, only the nucleic acid probe carries a fluorescence signal without generating a non-specific fluorescence signal, the generation of a supersaturated fluorescence signal can be effectively prevented by using the nucleic acid probe with a specific concentration, the fluorescence signals among different targets do not interfere with each other, and the detection sensitivity is high. And multicolor fluorescence can be used at the same time.

The invention provides a capillary electrophoresis analysis method of serum amino acid. The capillary electrophoresis analysis method comprises the following steps: S1, preparing an operation buffer solution; s2, selection and pretreatment of a quartz capillary column; s3, conditions are analyzed through electrophoresis; s4, drawing a quasi curve; s5, sample derivatization; s6, quantitative analysis. According to the method, a mixed reagent of alpha-cyclodextrin, beta-cyclodextrin and gamma-cyclodextrin is adopted for the first time, the amino acid migration behavior is cooperatively adjusted, a migration time window is expanded, the separation resolution of amino acids with similar structures is effectively improved, and the separation degree and the atlas definition are remarkably improved. According to the invention, sodium taurocholate is added, pH is regulated and controlled, a weakly alkaline buffer system with moderate conductivity and stable migration behavior is formed, separation and detection of FITC derived amino acids are facilitated, and balance of migration time distribution is optimized. The method is suitable for scenes of clinical sample analysis, disease marker screening and the like, and has good universality, stability and popularization value.

The presently described and claimed disclosure relates to capillary electrophoresis methods for quantifying an intact AAV genome and protein components in an AAV using the same capillary electrophoresis system. The claimed and described approach offers an automated analysis of AAV samples and provides information to determine the AAV empty / full ratio.

Electrophoresis device, including: a sample dish (112) on which a positive-electrode-side buffer solution container (103) containing a buffer solution and a phorese medium container (102) containing a phorese medium are arranged, and which is driven in a vertical and a horizontal direction; a thermostat oven unit (113) which holds a capillary arrangement (101) with a capillary head (403) in which a plurality of capillaries (401) are bundled in a single unit at one end thereof in a state in which the capillary head (403) protrudes downwards, and which maintains a constant internal temperature; a solution dispensing mechanism (106) for dispensing the phorese medium in the phorese medium container (102) to the capillary assembly (101) from the capillary head (403); and a current source (408) for applying a voltage to both ends of the capillary arrangement (101), wherein Openings for inserting the capillary head (403) are provided in the upper areas of the positive electrode-side buffer solution container (103) and the phorese medium container (102), and the thermostat oven unit (113) comprises a first lid element (207) which is arranged above the sample dish (112) and seals the upper part of the positive electrode-side buffer solution container (103) during the dispensing of the phorese medium by the solution dispensing mechanism (106), wherein the upper part of the positive electrode-side buffer solution container (103) is sealed by raising the sample dish (112) and pressing the positive electrode-side buffer solution container (103) against the first lid element (207).

Provided is a capillary electrophoresis device in which a sample that contains a first component and a second component is injected into a capillary, the injected first component and second component are subjected to electrophoretic separation, and emission of light from the first component and the second component induced by irradiating the capillary with light is measured by a detector, whereby the signal intensity of the first component and the signal intensity of the second component are acquired, wherein: the concentration range of the first component contained in the sample includes, in addition to the concentration range in which the signal intensity of the first component is proportional to the concentration of the first component, a concentration range in which the signal intensity of the first component is lower than the saturation signal intensity of the detector and reaches the saturation signal intensity when disproportional to the concentration of the first component; and the capillary electrophoresis device quantifies the ratio of the concentration of the first component to the concentration of the second component in the sample on the basis of the ratio of the signal intensity of the first component to the signal intensity of the second component.

The invention discloses a tulip EST-SSR (expressed sequence tag-simple sequence repeat) molecular marker as well as a primer composition and application thereof. According to the invention, 15 pairs of tulip EST-SSR molecular markers and a primer composition thereof are obtained through screening, a to-be-detected tulip variety resource DNA is extracted, the 15 pairs of EST-SSR primers or synthetic fluorescent primers are respectively used for PCR amplification, a PCR product is subjected to PAGE detection or fluorescent capillary electrophoresis, and the EST-SSR fingerprint of the tulip variety resource is constructed. The method and the basis are provided for tulip variety breeding, variety authenticity identification, known variety library construction and the like.

The invention relates to an orthogonal multichannel capillary electrophoresis gene analyzer, which is characterized in that a fluorescence excitation module is arranged to form two laser beams which are coaxially and crosswise emitted into an array capillary from two sides of the array capillary, and an optical fiber module is arranged to collect fluorescence signals emitted by all channels of the array capillary; according to the invention, fluorescence emitted from two ends of an array capillary tube deviates from an optical axis, so that aberration caused by deviation of fluorescence emitted from two ends of the array capillary tube in an objective lens collection scheme is avoided, and the number of channels of the capillary tube can be further increased, so that the period of large-scale gene sequencing is shortened; and the application range of the multichannel capillary electrophoresis gene analyzer is expanded.

The application discloses a kind of molecular markers for identifying genetic sex of blue fish and its application, belong to the field of aquatic animal genetics and breeding.The molecular marker includes nucleotide sequence such as the common band of blue fish female and male fish shown in SEQ ID NO:1 and nucleotide sequence such as the unique band of blue fish male shown in SEQ ID NO:2, and the identification method comprises: taking the DNA of the blue fish sample to be identified as template for PCR amplification, after the band of PCR product is determined by agarose electrophoresis, capillary electrophoresis is carried out, if signal appears at 152bp and 168bp simultaneously, then it is male;If only signal appears at 168bp, then it is female.The molecular marker of the application can accurately obtain the genetic sex of different blue fish populations, has the advantages such as simple, fast, efficient, not affected by early undifferentiated gonad of blue fish, etc., is used for blue fish marker assisted breeding, which greatly saves the cost of aquaculture and improves the breeding efficiency.

The invention provides a method for detecting sulfonamides in animal-derived food through capillary electrophoresis-laser-induced fluorescence, and belongs to the technical field of detection. Comprising the following steps: (1) mincing a sample, adding an extracting solution for extraction, and carrying out solid-phase extraction column extraction and methanol elution to obtain an eluted sample solution; (2) preparing a sulfanilamide drug standard substance and fluorescamine into gradient solutions by using methanol and acetonitrile respectively, and stirring at room temperature for reaction to obtain a solution A; (3) mixing the elution sample solution with a fluorescent amine-acetonitrile solution, and stirring and reacting at room temperature to obtain a solution B; and (4) detecting the fluorescence intensity of the sulfonamides in the solution A by adopting capillary electrophoresis-laser induced fluorescence to obtain a linear equation for detecting the concentration of the sulfonamides in the solution B. The invention establishes a novel method for detecting sulfonamide residues in animal-derived food by using high-efficiency capillary electrophoresis-laser-induced fluorescence, and realizes high-sensitivity detection of three sulfonamides in animal-derived food.

The present disclosure provides methods for assessing the integrity of inverted terminal repeat sequences in AAV plasmids by restriction digestion followed by capillary electrophoresis.

A DNA analysis system is provided with: a flow channel device having a PCR chamber in which a thermal cycle is performed; and a capillary electrophoresis unit that performs electrophoretic analysis on a PCR reaction solution, in which the DNA analysis system stores values of m and n that are set in advance, thermal cycles are performed m times on the PCR reaction solution in the PCR chamber to generate a first reaction solution, a part of the first reaction solution is taken out from the PCR chamber without changing the composition, and the first reaction solution is taken out from the PCR chamber without changing the composition of the first reaction solution. Wherein, in the PCR chamber, the first reaction solution remaining in the PCR chamber is subjected to n-m (n-m is an integer of 2 or more) thermal cycles such that the total number of thermal cycles becomes n, and a second reaction solution is generated, and the first reaction solution is subjected to electrophoretic analysis in the capillary electrophoresis unit, and the first reaction solution remaining in the PCR chamber is subjected to n-m (n-m is an integer of 2 or more) thermal cycles in the PCR chamber. At least a portion of the second reaction solution is taken out from the PCR chamber without changing the composition, and the at least a portion of the second reaction solution is subjected to electrophoretic analysis in the capillary electrophoresis unit.

The utility model relates to the capillary electrophoresis appearance field, concretely relates to a high -efficient capillary electrophoresis appearance with material detection, including material cabinet, the top of material cabinet is hanked with shell, the right side of shell inner chamber bottom is hanked with electrophoresis appearance, the left side of shell inner chamber bottom is hanked with water tank, the lower portion communication of water tank left side has first water supply pipe, the upper portion of shell left side is hanked with transverse board, the top of transverse board is hanked with air cylinder. The utility model discloses through water tank, first water supply pipe, transverse board, air cylinder, water collecting tank, first water pump etc. assembly, adds the cleaning component, is convenient for automatic cleaning to the detection pipe before and after the sample, when washing, only need to draw the liquid through first water supply pipe to the water collecting tank, through the water pressure and clean water flow extrude into the sampling needle, and through the mode of spraying to the detection pipe below flush, sets up first water pump on the sample tube, and the flow size is convenient for setting, and the sample number and total amount are convenient for controlling.

The present invention provides a, preferably fully automated and / or multiplex, method for the simultaneous detection of a plurality of molecular genetic analytes in a collective and continuous reaction set up and at least one analyte specific oligonucleotide TX probes with a cleavable hydrolysis product for use in said method. Through the method the plurality of cleavable hydrolysis products is specifically released by a nuclease from the respective TX probes. Following a separation step, preferably in a capillary electrophoresis, for each hydrolysis product a clear and distinguishable from others signal is achieved. Each separated hydrolysis product respectively results in a signal enabling the qualitative and / or quantitative detection of each analyte comprising a molecular variant, e.g. single nucleotide polymorphism (SNP), deletion-insertion polymorphism (DIP) or other, respectively, that was targeted specifically by the plurality of TX probes. Preferably, the method is suitable to detect qualitatively and / or quantitatively small molecular variants as such SNP.

Some embodiments described herein relate to capillary-containing cartridges suitable for use with capillary electrophoresis instruments. Embodiments described herein generally relate to cartridges that include a transfer capillary coupled to a separation capillary. The transfer capillary can be configured to be disposed in sample reservoirs and / or buffer reservoirs. Suction applied through a sheath interface of the transfer capillary and the separation capillary can draw sample / buffer from such reservoirs and bring the sample / buffer into contact with the separation capillary. The separation capillary can be configured for separation of analytes contained within the sample, for example when an electric potential (i.e., voltage) is applied across the separation capillary.

The invention belongs to the technical field of molecular detection, and particularly relates to a respiratory tract pathogen detection kit based on capillary electrophoresis and application of the respiratory tract pathogen detection kit, the kit can detect common pathogens which are possibly infected by respiratory tract patients and cannot be detected and determined through a conventional technology or a kit, and the kit at least has the technical advantages of being high in sensitivity and specificity.

This invention describes systems, primers, kits, and methods for detecting microsatellite instabilities in biological samples. Signal data is received from a capillary electrophoresis genetic analysis instrument, wherein the signal data is determined based on the fluorescence of fragments comprising nucleic acid sequences amplified from the biological sample by polymerase chain reaction (PCR). The nucleic acid sequences correspond to multiple different microsatellite loci and are obtained using multiple PCR primers configured to side-join the multiple microsatellite loci of the biological sample. When the PCR primers are combined with the biological sample and subjected to PCR amplification, fluorescently labeled DNA fragments comprising the multiple microsatellite loci are generated. The microsatellite instabilities of the biological sample are classified using the fluorescence data obtained from the multiple fluorescently labeled microsatellite loci.

The present invention provides a primer combination, a kit, a detection system, a detection method and an application for detecting pathogenic gene mutations of familial Alzheimer's disease and APOE genotypes by means of constant temperature multiplex rapid detection. The method of the present invention uses the amplification refractory mutation system technology combined with the nicking endonuclease nucleic acid detection reaction isothermal amplification system to perform multiplex amplification on 14 sites including 12 hot spot mutations and 2 risk sites of the Alzheimer's disease pathogenic gene, and at the same time combines capillary electrophoresis for product analysis to perform genotyping detection.

The present disclosure provides a capillary electrophoresis device including a light source, a first irradiation fiber configured to guide light from the light source, a detector configured to detect light from a capillary, and a first detection fiber configured to guide light to the detector to make an optical light detection system less susceptible to an external environment. A capillary cartridge including a capillary, a second irradiation fiber, and a second detection fiber is attached to the capillary electrophoresis device by connecting the first irradiation fiber and the second irradiation fiber, and connecting the first detection fiber and the second detection fiber. In the capillary cartridge, the second irradiation fiber and the second detection fiber are fixed such that their optical axes intersect each other in the lumen of the capillary.

The invention relates to the technical field of molecular markers, and particularly provides a kit for identifying the genotyping of a functional marker of a waxy gene of a corn variety. The multi-marker multi-detection platform compatible kit provided by the invention contains a primer combination as shown in SEQ ID NO.1-14, can detect and distinguish waxy genes wx-D7, wx-D10, wx-124 and wx-hAT at the same time, significantly improves the accuracy and efficiency of corn waxy gene detection, can be used on different molecular detection platforms, and has broad application prospects. Such as a KASP high-throughput platform and a capillary electrophoresis platform. According to the double-platform compatible kit provided by the invention, the waxy corn breeding period is greatly shortened, the breeding efficiency and accuracy are improved, and the use cost and the technical learning threshold are reduced, so that waxy corn breeding, variety identification and germplasm resource management are quicker and more accurate.

A method and gel buffer for capillary electrophoresis (CE) separation of proteins. The gel buffer solution comprises a buffer component, a hydrophilic polymer, an organic additive, a detergent and a metal chelating agent. By adopting a gel buffer solution, the separation degree of a main peak and an acromion can be improved when protein is separated by capillary electrophoresis (CE).

The present application relates to the technical field of forensic evidence, and particularly relates to a tumor tissue source identification method based on a second-generation sequencing platform. The present application uses the second-generation sequencing technology to count and statistically analyze the typing results of 15 STR markers. Compared with the commonly used STR marker combination based on capillary electrophoresis, the present application has better stability, higher accuracy and is more suitable for precise tracing of tumor tissues. The analysis method is simple, the influence of background signal does not need to be considered, and the result is easier to interpret. The present application can be used for tumor tissue source identification based on the second-generation sequencing platform.

The present disclosure provides a method for analyzing proteins and polypeptides by electrophoresis using standards as internal standards. In one aspect, the present disclosure provides a method for measuring an analyte by electrophoresis, the method including the steps of preparing standards that do not contain tryptophan, subjecting a sample containing the analyte and the standards to electrophoresis simultaneously in the same separation field, detecting the analyte with an optical signal derived from tryptophan and detecting the standards with an optical signal of a wavelength different from that of the analyte, and measuring the analyte based on the optical signal. In one embodiment, the electrophoresis is capillary electrophoresis.

The purpose of the present invention is to provide a capillary electrophoresis device that can confirm optical performance without performing a specialized task, and an optical performance diagnostic method for the capillary electrophoresis device. In order to achieve said purpose, the present invention is a capillary electrophoresis device comprising: a capillary array formed by a plurality of capillaries; a light source that oscillates laser light; a detector that detects light emitted when the capillary array is irradiated with the laser light; and a control unit that performs prescribed processing on the basis of signals from the detector. The control unit extracts a prescribed absolute value related to an optical index on the basis of an image captured by the detector, and calculates the optical index by comparing the extracted absolute value and a predetermined reference value.

A method for characterizing proteins in a biological sample is provided herein that includes adding one or more protein-binding agents, having a surface capable of binding proteins, to the biological sample and allowing a protein corona, including one or more proteins, to form on the surface of the one or more protein-binding agents to produce a complex including the protein corona and the one more protein-binding agents; separating the complex from the biological sample to generate a complex sample comprising the complex; separating the one or more protein-binding agents from the protein corona in the complex sample to generate a protein corona sample; and characterizing the proteins in the protein corona sample with Capillary Electrophoresis (CE) and Tandem Mass Spectrometry (MS / MS). Also provided herein are methods for detecting biomarkers and diagnosing a disease in a subject following the described characterization method.

In one aspect, a dual-mode capillary electrophoresis system is disclosed, which comprises a plurality of capillaries for receiving a plurality of samples, a UV radiation source for generating UV radiation along a first path, a laser light source for generating laser radiation along a second path, and a galvanometric mirror configured to receive radiation from said UV radiation source along said first path and to receive light from said laser light source along said second path, and to direct said received UV radiation and said laser light onto a common optical path, said galvanometric mirror further being configured to scan said UV radiation and said laser light sequentially over said plurality of capillaries. The system can further include detectors for detecting the UV radiation as well as fluorescent radiation emitted by the samples in response to laser excitation.