26 results about "Capillary electrophoresis" patented technology

The utility model relates to the capillary electrophoresis appearance field, concretely relates to a high -efficient capillary electrophoresis appearance with material detection, including material cabinet, the top of material cabinet is hanked with shell, the right side of shell inner chamber bottom is hanked with electrophoresis appearance, the left side of shell inner chamber bottom is hanked with water tank, the lower portion communication of water tank left side has first water supply pipe, the upper portion of shell left side is hanked with transverse board, the top of transverse board is hanked with air cylinder. The utility model discloses through water tank, first water supply pipe, transverse board, air cylinder, water collecting tank, first water pump etc. assembly, adds the cleaning component, is convenient for automatic cleaning to the detection pipe before and after the sample, when washing, only need to draw the liquid through first water supply pipe to the water collecting tank, through the water pressure and clean water flow extrude into the sampling needle, and through the mode of spraying to the detection pipe below flush, sets up first water pump on the sample tube, and the flow size is convenient for setting, and the sample number and total amount are convenient for controlling.

This invention describes systems, primers, kits, and methods for detecting microsatellite instabilities in biological samples. Signal data is received from a capillary electrophoresis genetic analysis instrument, wherein the signal data is determined based on the fluorescence of fragments comprising nucleic acid sequences amplified from the biological sample by polymerase chain reaction (PCR). The nucleic acid sequences correspond to multiple different microsatellite loci and are obtained using multiple PCR primers configured to side-join the multiple microsatellite loci of the biological sample. When the PCR primers are combined with the biological sample and subjected to PCR amplification, fluorescently labeled DNA fragments comprising the multiple microsatellite loci are generated. The microsatellite instabilities of the biological sample are classified using the fluorescence data obtained from the multiple fluorescently labeled microsatellite loci.

The invention provides a high-flux monosaccharide separation and quantification method and kit based on gel electrophoresis. The separation and quantification method comprises the following steps: (1) providing a fluorescence-labeled monosaccharide sample to be detected; (2) injecting the monosaccharide sample to be detected into a capillary electrophoresis system, and performing first electrophoretic separation under a first separation system to obtain a first electrophoretogram; the first separation system comprises borate-free first separation gel and a first buffer solution; (3) cleaning a capillary tube of the capillary electrophoresis system, injecting the monosaccharide sample to be detected into the capillary tube again, and performing electrophoretic separation for the second time in a second separation system to obtain a second electrophoretogram; the second separation system comprises second separation gel containing borate and a second buffer solution; and (4) carrying out qualitative identification and quantitative calculation on the mixed monosaccharide according to the migration time difference and peak overlapping condition of each monosaccharide component in the first electrophoretogram and the second electrophoretogram.

The application discloses a kit for detecting 87 respiratory tract pathogens and drug-resistant genes by using a single tube and application thereof. The kit comprises specific primers and probes for the 87 respiratory tract pathogens and drug-resistant genes and is based on a capillary electrophoresis platform. The kit can detect nearly 100 targets and has the advantages of high throughput, high sensitivity, high specificity, simple operation, short TAT time, simple result interpretation and the like, and is suitable for rapid screening and clinical diagnosis of respiratory tract pathogens and drug-resistant genes.

The present invention relates to a method for purifying labeled glycans by electroosmotic flow assisted capillary zone electrophoresis, or a method for on-line electrokinetic purification of a labeled reaction mixture of labeled glycans and free labeling agent, wherein the method is performed in a separation mode, wherein the free labeling agent and the labeled glycans migrate in opposite directions, thereby separating the free labeling agent from the glycans. The method according to the present invention allows for a purification-free capillary zone electrophoresis analysis of labeled glycans. Furthermore, the method allows for separation in a shorter analysis time without the need for a sample purification step.

This invention belongs to the field of microbial application technology, specifically relating to a method for preparing fermented *Lactobacillus limnoxicamis* extracellular vesicles, the product, and its applications, particularly the application of these fermented *Lactobacillus limnoxicamis* extracellular vesicles and their lyophilized formulations in the preparation of products with antioxidant or anti-aging effects. In this invention, *Lactobacillus limnoxicamis* is inoculated into a modified extracellular vesicle induction medium for fermentation. After culture, a two-stage solid-liquid separation is performed, and the supernatant is collected to obtain the extracellular vesicle processing solution. This solution is purified using a capillary electrophoresis system, and the positive extreme liquid is collected and filtered to obtain the purified product of fermented *Lactobacillus limnoxicamis* extracellular vesicles. The extracellular vesicles derived from fermented *Lactobacillus limnoxicamis* prepared by this invention can effectively scavenge free radicals. Somatic cell experiments have demonstrated its excellent anti-aging properties, and the transformation and application research of *Lactobacillus* extracellular vesicles has explored its development potential in the field of anti-aging products.

The application discloses a primer, a kit and a method for identifying Curcuma plants, and belongs to the technical field of species identification. The method comprises the following steps: (1) extracting DNA from Curcuma plants to be identified; (2) using the DNA obtained in step (1) as a template, performing PCR amplification on the template by using 26 pairs of primers to obtain amplification products; (3) performing fluorescence capillary electrophoresis detection on the amplification products obtained in step (2); and (4) analyzing the detection results obtained in step (3) by using data analysis software, performing cluster analysis on sample genotype data obtained in the analysis, determining whether the Curcuma plants to be identified can be clustered into one category with the Curcuma plants in a cluster map, and distinguishing different individuals of the Curcuma plants according to a cluster result. The primer and the kit can be used to realize rapid identification of the Curcuma plants, and can be used to identify Curcuma kwangsiensis, C. zedoaria, C. longa, C. wenyujin, C. rotundifolia and C. aromatica.

The application relates to a capillary electrophoresis system and method combined with a non-continuous sample injection mass spectrum, wherein the system comprises: an electrophoretic separation-electrospray ionization module for separating components in a target analyte according to different electrophoretic mobilities; a real-time adjustable high-voltage power supply device for providing a voltage required by capillary electrophoresis according to the requirements of an electroosmotic flow and an electrophoretic speed in a capillary; a target component signal feedback module for detecting a target analyte or a real-time electroosmotic flow speed; an electrophoretic current measurement and migration time correction module; a real-time feedback signal processing and control module; and a non-continuous sample injection mass spectrum system. Thus, the problems in the prior art that, when a continuous capillary electrophoresis technology is combined with a non-continuous sample injection small mass spectrum system, the accuracy is reduced, the analysis quality is poor, the sampling frequency is greatly limited, it is difficult to ensure that mass spectrum data at the highest point of a chromatographic peak can be collected, and the sensitivity of detection and the accuracy of quantitative analysis are affected are solved.

A method to quantity clay content may comprise flowing a rock sample with water, wherein the water is collected, and a capillary electrophoresis analysis or any water analysis method is run on the water. The method further comprises calculating a clay integrated area using constructed water analysis peaks. Lastly, a correlation to quantify the clay content in the rock sample, and another correlation to quantify the clay content in the water sample are formulated.

The application provides an automatic electrophoresis device based on a micro-pump valve integrated chip, and belongs to the technical field of capillary electrophoresis. The device comprises an electrophoresis chip and at least one glue filling control unit. The electrophoresis chip has a micro-pump valve unit, which comprises a glue filling plunger and a plunger valve for controlling the opening and closing of a flow path. The glue filling control unit is provided with corresponding plunger driving elements and valve core driving elements. The glue filling control unit can slide relative to the electrophoresis chip, so as to be switched between a first coupling position and a first decoupling position. Through the sliding coupling and decoupling between the driving mechanism and the chip, the application solves the problem that the chip consumables are difficult to replace due to the fixed connection of the driving assembly. Meanwhile, through decoupling avoidance, the spatial interference of the driving mechanism on the sample injection module, the detection module and other modules in different process stages is eliminated, and the overall automation level of the device is improved.

This invention relates to the field of biotechnology and discloses a multiplex fluorescent PCR detection system and its applications. The multiplex fluorescent PCR detection system includes 22 primer pairs, and discloses primer sequences targeting 22 sites, used to simultaneously amplify 15 detection sites and 7 internal reference sites. The multiplex fluorescent PCR detection system provided by this invention achieves effective detection of multiple methylation sites by combining capillary electrophoresis detection. Combined with corresponding detection methods and result interpretation methods, it enables highly sensitive and specific detection of colorectal cancer, possessing significant clinical application value and broad market prospects.

The application relates to a kind of four seasons antiviral mixture multi-component high-efficiency detection methods based on capillary electrophoresis, comprising: S1, standard solution preparation;S2, borax buffer preparation;S3, standard curve establishment;S4, sample pretreatment;S5, sample injection detection.The application realizes the high-efficiency separation of four seasons antiviral mixture by high-performance capillary electrophoresis (HPCE), and the sample consumption is less, environment-friendly, suitable for the rapid analysis of multi-component in traditional Chinese medicine complex system.

The application discloses a SSR molecular marker primer combination and method for identifying Morchella sexta ZJYDJ001 strain, and the SSR molecular marker primer combination comprises four pairs of SSR molecular marker primers, namely MSSR029, MSSR036, MSSR041 and MSSR092. The method for identifying the Morchella sexta ZJYDJ001 strain based on the SSR molecular marker primer combination is realized according to the following steps: firstly, extracting mycelium genomic DNA of the Morchella sexta strain to be detected; then, performing PCR amplification on the DNA by using the SSR molecular marker primer combination; performing fluorescent capillary electrophoresis detection on the amplified PCR product; and comparing the fluorescent peak diagram, so that the Morchella sexta ZJYDJ001 strain can be identified. The method provided by the application can be used for rapidly identifying the Morchella sexta strain at the mycelium stage, avoids mixing the fine strain selected through breeding with other Morchella sexta strains in the production and operation and market flow process, thereby effectively protecting intellectual property rights, and has important significance for identifying the authenticity of the Morchella sexta strain in the production process.

This invention discloses a molecular biology method and its application for simultaneously detecting five poultry-derived components. Using primers shown in SEQ ID NO.1 and SEQ ID NO.2, genomic DNA of the sample to be tested is amplified by PCR. After capillary electrophoresis, sequence alignment is performed. A peak value of 234 bp indicates a chicken-derived component; a peak value of 271 bp indicates a quail-derived component; a peak value of 229 bp indicates a turkey-derived component; a peak value of 252 bp indicates a partridge-derived component; and a peak value of 223 bp indicates a pheasant-derived component. Peak values ​​of 234 bp, 271 bp, 229 bp, 252 bp, and 223 bp indicate the simultaneous presence of five poultry-derived components. The detection kit developed based on this method can generate considerable economic benefits and good social value.

This application belongs to the field of molecular detection technology, specifically relating to a detection kit for respiratory pathogens and drug resistance genes based on a capillary electrophoresis detection platform and its application. The kit simultaneously detects the pathogens infecting patients with respiratory tract infections and the possible drug resistance genes through a single tube reaction. Compared with traditional methods, this application covers common respiratory pathogens and drug resistance genes, with more comprehensive detection sites, higher specificity, better sensitivity, and stronger reliability, and has the capability for batch detection.

The present invention relates to roadside analyzer for determination of illegal drugs abuse, including, but not limiting to detection of explosives, toxic industrial chemicals and other banned or regulated compounds, biomarkers and phytochemicals in a sample in situ in at least one human body fluid sample, specifically in oral fluid (saliva), but not limiting to other clinical samples of interest (urine, blood, exhaled breath, exhaled breath condensate, etc.) It consists of automatic processor for preparing samples suitable for analysis. Analysis part of the instrument implements three technologies, namely solid phase extraction prior to analysis, capillary electrophoresis for separation of analytes from the sample matrix and impedance (contactless conductivity) or fluorescence or both impedance (contactless conductivity) and fluorescence for detection of analytes of interest.

The application discloses a kind of multi-channel integrated capillary electrophoresis chip and flow path control method, belong to detection chip technical field.It includes: chip ontology, which is provided with sample inlet through hole, separation channel, plunger valve port, plunger valve channel;Sample inlet through hole is communicated with separation channel and is used for sample introduction;Separation channel is provided with multiple, any one separation channel is configured with a sample inlet through hole, plunger valve channel is communicated with separation channel by plunger valve port;Separation channel includes straight line section, first curve extension section and second curve extension section, and the total length of the straight line section, the first curve extension section and the second curve extension section of each separation channel is the same.Through integrating multiple separation channels with equal total length, each channel is equipped with independent sample inlet through hole and detection window, multi-channel synchronous detection is realized, and the unit length electric field intensity of each separation channel is consistent, effectively eliminates migration time drift, and improves multi-channel detection consistency.

This invention discloses a quantitative sample injection device and method for capillary electrophoresis, applied in the field of capillary electrophoresis technology. The key technical points are: it includes a sample vial and a buffer chamber, with a capillary tube fixedly connected between the sample vial and the buffer chamber; a three-way valve is also fixedly provided, with the capillary tube and the buffer chamber respectively fixedly connected to the three-way valve; a quantitative sample injection component for generating negative pressure to achieve sample injection is externally fixedly connected to the three-way valve; a shut-off valve is fixedly connected between the three-way valve and the buffer chamber; and a miniature stopcock valve is fixedly provided between the quantitative sample injection component and the three-way valve. The technical effect is: improving the accuracy of sample injection volume while reducing the cost of the injection device.

The application discloses a detection method of breast milk oligosaccharide. The application provides a detection method of breast milk oligosaccharide, which comprises the step of detecting a to-be-detected sample by using a capillary electrophoresis-laser-induced fluorescence-based detection method, wherein the running buffer used in the capillary electrophoresis-laser-induced fluorescence-based detection method comprises Tris-base, EDTA, urea, and more than 2% (m / V) and less than 4% (m / V) PVP and more than 5% (m / V) and less than 15% (m / V) boric acid. The detection method provided by the application can be applied to the separation and detection of acidic and neutral HMOs isomers in the to-be-detected sample (for example, breast milk and infant formula milk powder samples).

The invention discloses an SNP (Single Nucleotide Polymorphism) genetic marker system for genetic relationship identification of a highly degraded sample, a detection primer and application of the SNP genetic marker system and the detection primer, and belongs to the technical field of forensic medicine identification. According to the invention, through bioinformatics screening and experimental verification, a triallelic SNP genetic marker system of which the lengths of 25 amplicons all do not exceed 110 bp is constructed. The problem that it is difficult to give consideration to genetic marker typing and high system efficiency of highly degraded samples is solved. The random matching probability of the system in Han population in Hunan reaches 1.59 * 10 <-18 >, the cumulative paternity exclusion rate is 0.99979, complete typing of highly degraded DNA can be realized, triad paternity test can be completed, and a new technical scheme which is efficient and compatible with a capillary electrophoresis platform is provided for forensic practice.

A molecular biology identification method and application of different small white-feathered broilers, the molecular marker is that the genomic DNA of the small white-feathered broiler is amplified by using the sequence shown in SEQ ID NO. 1 and SEQ ID NO. 2, and the alleles are detected by capillary electrophoresis, the small white-feathered broiler containing 205 bp alleles is F type small white chicken, and the small white-feathered broiler not containing 205 bp alleles is M type small white chicken; the present application identifies different small white-feathered broilers by using the difference of the alleles, and the advantage lies in that the F type and M type small white chicken can be quickly and accurately identified. Moreover, the method is simple in operation, easy for laboratory operation, convenient for grass-roots promotion and use, has a wide market application prospect, in addition, the detection kit developed based on the method of the present application can produce considerable economic benefits and good social value.

The invention belongs to the technical field of molecular detection, and particularly relates to a respiratory tract pathogen detection kit based on capillary electrophoresis and application of the respiratory tract pathogen detection kit, the kit can detect common pathogens which are possibly infected by respiratory tract patients and cannot be detected and determined through a conventional technology or a kit, and the kit at least has the technical advantages of being high in sensitivity and specificity.

The application provides a molecular marker primer for molecular identification of an oil olive purple gold variety and application, and belongs to the field of molecular identification of the oil olive purple gold variety. Preparing materials, extracting DNA of a to-be-tested sample, amplifying a product of sample PCR, detecting fluorescence capillary electrophoresis, and identifying the sample by comparing peak values. The specific primer is used for PCR amplification, the amplified product is detected by fluorescence capillary electrophoresis, and the peak values of the sample amplification product are compared with the standard peak values of the specific primer, so that whether the test sample is the oil olive purple gold variety is quickly and effectively judged, and the work of hybrid breeding, variety protection and production technology popularization of the oil olive is facilitated.