9 results about "Microfluidic Analysis" patented technology

The present disclosure relates to microfluidic analysis of biological samples. The present disclosure features methods and systems for processing a sample, the method including introducing a sample characterized by one or more components in a first electrolyte solution into a separation channel of a fluidic device; using an electrode for applying a potential difference across the sample to cause migration of at least one sample component toward an end of the separation channel; and adjusting at least one of a gas pressure in a reservoir comprising a second electrolyte solution and a gas pressure external to an orifice located at the end of the separation channel, such that the gas pressure in the reservoir is greater than the gas pressure external to the orifice; and directing a flow of the second electrolyte solution through a pumping channel and out of the orifice in response to the gas pressure to expel at least one sample component comprising a background electrolyte solution from the separation channel and the pumping channel through the orifice.

A continuous analysis method comprising generating microfluidic droplets comprising multiple copies of a same antigen, multiple copies of a same primary antibody potentially capable of specifically binding to said antigen, a multivalent crosslinking agent being a biomolecule or a support, and a fluorescent labeling agent, said continuous microfluidic analysis being a fluorescence-based sorting.

The application provides a wearable sweat micro-fluidic analysis chip and a preparation method and application thereof. The wearable sweat micro-fluidic analysis chip is sequentially provided with a sweat inlet layer 1, a branch channel layer 3 and a cover layer 5 from top to bottom. The sweat inlet layer is provided with a sweat inlet 6. The branch channel layer 3 is provided with a detection chamber 10, a channel 17 and a valve chamber 9. The sweat inlet 6, the valve chamber 9, the channel 17 and the detection chamber 10 are sequentially communicated. The cover layer 5 is provided with a sweat outlet 14. A water droplet type polymer valve is placed in the valve chamber 9. The opening time of the water droplet type polymer valve can be flexibly controlled by simply adjusting the polymer concentration. The sweat sample can be sequentially collected, and the contact time of the sweat sample and the detection reagent can be accurately controlled. A reliable premise is provided for subsequent accurate analysis. The application can be applied to the detection of cortisol and the detection of uric acid.

A pressure supply unit for providing a first target pressure level and a second target pressure level, includes a pump for drawing in a fluid, a first fluid flow control component, and a second fluid flow control component. The first fluid flow control component is arranged on a first side of the pump that controls the fluid flow according to a first current pressure level and is thus configured to provide the first target pressure level on the first side of the pressure supply unit. The second fluid flow control component is arranged on a second side of the pump that controls the fluid flow according to a second current pressure level and is this configured to provide the second target pressure level on the second side of the pressure supply unit.

The application belongs to the technical field of urea nitrogen detection, and particularly relates to an integrated microfluidic chip device and method for detecting urea nitrogen; wherein a cover plate is bonded to a main plate, and the main plate is bonded to a base plate; the main plate is provided with a light path channel, a micro flow channel, a reaction chamber, a sample chamber, a control chamber and a groove; a photoelectric detector is fixed in the groove, an LED light source, a lens, a spectroscope and a reflecting mirror are fixed in the light path channel, the sample chamber and the reaction chamber are communicated through the micro flow channel, and the sample chamber and the outside are communicated through the micro flow channel; the cover plate is etched with an injection port communicated with the reaction chamber and the control chamber; the application adopts the microfluidic analysis chip idea, and through microfabrication technology, functional elements such as the LED light source, the lens, the spectroscope, the reflecting mirror, the reaction chamber, the sample chamber, the control chamber, the micro flow channel, the photoelectric detector and the main control chip are integrated on the chip like integrated circuits, and the idea of transferring the functions of the whole laboratory to the chip is concentratedly embodied.

The application discloses a kind of microfluidic titration device and titration method, it is related to microfluidic analysis detection technical field, including base, white background plate, black background plate, vertical column, horizontal beam, test tube clamp, titration test tube, background light box, background lamp, light shield, base level, adjustable foot, beam horizontal adjusting mechanism and beam micro level, automatic control unit, microfluidic flow control module, titration end point image recognition module, background driving module, data processing storage module;The bottom of base is provided with adjustable foot, base level is arranged on base, vertical column is fixed on base, horizontal beam is connected with vertical column by beam horizontal adjusting mechanism, beam micro level is fixed on horizontal beam, and test tube clamp is arranged on horizontal beam.A kind of microfluidic titration device and titration method, through the double leveling structure of base and beam, add automatic control module to realize intelligent regulation and control of whole process, eliminate benchtop and assembly error, and the batch detection efficiency of multi-channel clamping is improved.

The invention provides an edible mushroom heavy metal detection system, method and device, and relates to the technical field of food safety detection.The system comprises a detection cassette used for positioning a paper-based microfluidic analysis card and a smart phone slot; the paper-based micro-fluidic analysis card is provided with a filter layer, a solid-phase reagent release layer and a detection array layer which are sequentially arranged along the liquid permeation direction; a detection application program is installed on the smart phone; the detection method comprises the following steps: automatically completing filtration, reagent mixing and chromogenic reaction of a bacterial sample extracting solution through the analysis card, carrying out photographing and image analysis on a detection array area of the analysis card by using the smart phone arranged in the cassette, and calculating the heavy metal concentration; by combining the low-cost consumables with the smart phone, rapid, simple and low-cost field quantitative detection of the heavy metals in the edible mushrooms is realized, the operation is convenient, and the result is reliable.

The application relates to the technical field of microfluidics, and discloses a modular tubular microfluidic analysis and detection device and a detection method, which comprises the following steps: manufacturing a fluid input module and a reaction and measurement module by using laser cutting, machining, injection molding or 3D printing technology; the fluid input module comprises a plurality of fluid input mechanisms; the fluid input mechanism comprises a liquid storage device, a first micropump and a first microvalve, and each fluid input mechanism is independent of each other; the reaction and measurement module comprises a microreactor and a signal reader; the signal reader is connected with the microreactor; a hose is used to connect the fluid input module and the microreactor; the liquid to be detected is stored in the liquid storage device; the liquid to be detected flows into the microreactor through the hose under the control of the first micropump and the first microvalve; the signal reader acquires signal changes when the liquid to be detected in the microreactor reacts; and analysis and detection results are obtained according to the signal changes. The application can realize a stable pipeline structure, facilitate function expansion and reduce costs.

The invention relates generally to biochemical assays and more particularly to digital microfluidics multi-dynamic range parallel biochemical assays. The invention provides methods that enable improved precision and linearity for digital microfluidics analyses (i.e., analyses performed on a droplet actuator using droplet operations), including for example, assays related to measuring analytes in a biological sample. Examples of assays that may be performed using a method of the invention include measuring analytes in blood, including analytes from blood components, such as analytes present in plasma. The invention provides a digital microfluidics device (or cartridge) and methods for performing a biochemical assay using the microfluidics device to measure an analyte in a sample.