51 results about "Microfluidic Analysis" patented technology

A system for detecting microbial contamination in food processing environments, comprising: a sensor array with a variety of biosensors for detecting microbial markers that indicate a specific microbial contamination; a microfluidic analysis unit operatively coupled to the sensor array, the microfluidic unit comprising microchannels that direct environmental or food sample fluids through a series of analyzable stages, including sample concentration, enrichment, and pathogen identification; and a central processing unit (CPU) communicatively connected to the sensor array and the microfluidic device, the CPU being configured to receive and process real-time data from the sensor array, apply machine learning techniques to detect microbial contamination, and trigger an automated response based on predetermined contamination severity thresholds, the system being designed to continuously monitor microbial contamination at various stages of food processing, providing operators with real-time feedback and enabling immediate corrective action.

The invention relates to a microfluidic analysis device and method for electrochemical luminescence immunoassay detection, the device comprises a chip body and a heater, the chip body comprises a functional area, a capillary channel, an air connection channel, a detection chamber, an air pressure chamber and a three-electrode sensor, a fluid flowing unit with a height difference and a capillary structure is constructed on the basis of the chip body, every two adjacent functional areas are communicated through a corresponding air connecting channel, the capillary main channel is communicated with the detection cavity, the capillary main channel is provided with a plurality of branch pipe sections which are communicated with the corresponding functional areas one by one, and the branch pipe sections are communicated with the detection cavity. The capillary auxiliary channel enables the detection chamber to be communicated with the air pressure chamber, the three-electrode sensor is arranged in the detection chamber, and the heater is arranged on the back face of the air pressure chamber. According to the invention, dependence on an injection pump or an external mechanical clamp is eliminated, accurate control and automatic operation of liquid in the micro-fluidic chip are realized, and high-sensitivity biomolecule detection is completed on the micro-fluidic chip.

The invention belongs to the technical field of microfluidic detection, and relates to microfluidic analysis equipment which comprises a lower shell, an outer shell, an inner lower shell, an inner upper shell, a kit mounting seat, a driving device, a magnet moving structure, a pressing structure and other core components. A kit mounting seat in the inner lower shell can be driven by a driving device to slide, a magnet moving structure drives a magnet to move along a magnet slideway of the kit to promote a micro-flow reaction, and a pressing structure presses a pressing part of the kit to assist the reaction; meanwhile, structures such as slide rail and slide block matching, motor lead screw transmission, two-dimensional code scanning, light path adjustment and temperature control are integrated, so that accurate positioning, intelligent adaptation and stable reaction environment of the kit are realized, and the detection accuracy is improved. The problems that an existing device is single in driving mode and insufficient in detection precision are solved, and the device is compact in structure, high in universality and capable of being widely applied to the fields of medical diagnosis, environment monitoring and the like.

The invention discloses a large-scale closed public place aerosol virus enrichment monitoring system based on an STM32 main control platform, which combines an air sampler and a microfluidic analysis technology, adopts a temperature and humidity real-time monitoring module, optimizes an air sampling and virus enrichment process, and realizes that an integrated microfluidic analysis unit completes rapid determination of virus concentration. According to the system, virus concentration detection is carried out on an enriched sample by utilizing a double-antibody sandwich enzyme-linked immunoassay technology, and the virus concentration is determined through absorbance measurement. Experiments show that the detection error of the system is lower than 5%, and virus monitoring which is quick in response, convenient to operate and low in cost can be achieved. The system is suitable for monitoring viruses such as influenza, new coronavirus and norovirus in large closed public places, and provides efficient technical support for public health safety.

The invention discloses a paper-based microfluidic analysis device for beta-lactoglobulin detection as well as a preparation method and application of the paper-based microfluidic analysis device, and relates to the technical field of food detection. The paper-based microfluidic analysis device comprises a bottom plate and absorbent paper loaded on the bottom plate, the absorbent paper sequentially comprises a sample application area, an acidification area containing a buffer system with the pH range of 3-5, a deposition area overlaid with at least one layer of absorbent paper, a neutralization area containing a buffer system with the pH range of 6-8, and a detection area containing a fluorescent probe for detecting beta-lactoglobulin along the liquid flowing direction; the structure of the fluorescent probe is as follows: R1 and R2 are selected from hydrogen or C1-C5 alkoxy, and R3 is methyl or ethyl. When the paper-based microfluidic analysis device is applied, a detection sample passes through the acidification area to trigger casein precipitation, the deposition area is subjected to mechanical filtration, the neutralization area recovers the physiological pH condition before detection, and finally the detection area is combined with the fluorescent probe to generate a fluorescence detection signal.

The present disclosure relates to microfluidic analysis of biological samples. The present disclosure features methods and systems for processing a sample, the method including introducing a sample characterized by one or more components in a first electrolyte solution into a separation channel of a fluidic device; using an electrode for applying a potential difference across the sample to cause migration of at least one sample component toward an end of the separation channel; and adjusting at least one of a gas pressure in a reservoir comprising a second electrolyte solution and a gas pressure external to an orifice located at the end of the separation channel, such that the gas pressure in the reservoir is greater than the gas pressure external to the orifice; and directing a flow of the second electrolyte solution through a pumping channel and out of the orifice in response to the gas pressure to expel at least one sample component comprising a background electrolyte solution from the separation channel and the pumping channel through the orifice.

A continuous analysis method comprising generating microfluidic droplets comprising multiple copies of a same antigen, multiple copies of a same primary antibody potentially capable of specifically binding to said antigen, a multivalent crosslinking agent being a biomolecule or a support, and a fluorescent labeling agent, said continuous microfluidic analysis being a fluorescence-based sorting.

The application provides a wearable sweat micro-fluidic analysis chip and a preparation method and application thereof. The wearable sweat micro-fluidic analysis chip is sequentially provided with a sweat inlet layer 1, a branch channel layer 3 and a cover layer 5 from top to bottom. The sweat inlet layer is provided with a sweat inlet 6. The branch channel layer 3 is provided with a detection chamber 10, a channel 17 and a valve chamber 9. The sweat inlet 6, the valve chamber 9, the channel 17 and the detection chamber 10 are sequentially communicated. The cover layer 5 is provided with a sweat outlet 14. A water droplet type polymer valve is placed in the valve chamber 9. The opening time of the water droplet type polymer valve can be flexibly controlled by simply adjusting the polymer concentration. The sweat sample can be sequentially collected, and the contact time of the sweat sample and the detection reagent can be accurately controlled. A reliable premise is provided for subsequent accurate analysis. The application can be applied to the detection of cortisol and the detection of uric acid.

The invention relates to an analysis cartridge (1) for the detection and / or analysis of a species likely to be present in a liquid. The analysis cartridge (1) is provided with a front face and a rear face opposite the front face. The cartridge further comprises: - at least one microfluidic analysis chamber (5) for receiving the liquid, the at least one microfluidic chamber being formed within the cartridge and comprising a base (5a); - at least one first cluster (9) formed of magnetic nanoparticles held together and onto which capture agents are grafted, said capture agents being configured to bind specifically with the species, the at least one first cluster (9) being adherent to the base (5a). Figure 4b

A pressure supply unit for providing a first target pressure level and a second target pressure level, includes a pump for drawing in a fluid, a first fluid flow control component, and a second fluid flow control component. The first fluid flow control component is arranged on a first side of the pump that controls the fluid flow according to a first current pressure level and is thus configured to provide the first target pressure level on the first side of the pressure supply unit. The second fluid flow control component is arranged on a second side of the pump that controls the fluid flow according to a second current pressure level and is this configured to provide the second target pressure level on the second side of the pressure supply unit.

The application belongs to the field of food safety detection, and particularly relates to a food hazard double-mode detection method based on a three-dimensional folded paper-based microfluidic analysis device; the steps are as follows: step one, preparation of fluorescence-enhanced upconversion nanomaterial; step two, surface biological functionalization of the upconversion nanomaterial; step three, preparation of a double-metal organic framework with peroxidase activity; step four, preparation of a double-metal organic framework modified by a target aptamer; step five, preparation of a nano probe formed by coupling of the upconversion nanomaterial and the double-metal organic framework; step six, preparation of a three-dimensional folded paper-based microfluidic analysis device; step seven, rapid detection of food hazards by using the three-dimensional folded paper-based microfluidic analysis device; taking chlorpyrifos as an example, rapid and accurate detection of the content of chlorpyrifos in food is realized; meanwhile, the three-dimensional folded paper-based microfluidic analysis device with double-mode output has the capability of on-site real-time detection of hazards, and has a good practical prospect.

The invention discloses a kind of portable microfluidic analysis chip, belong to the field of microfluidic technology, including: sequential release functional area, micro-reaction functional area and fluid diversion functional area;Sequential release functional area includes M-level liquid storage chambers;The bottom of each liquid storage chamber is provided with micropore, and each micropore is connected with the input end of micro-reaction functional area;Only the top of the first-level liquid storage chamber is communicated with the atmosphere, and a float microvalve is provided in the front M-1 level liquid storage chambers;The bottom of the front-level liquid storage chamber is connected with the top of the rear-level liquid storage chamber;Fluid diversion functional area includes drainage flow channel, the recovery branch flow channel for recovering reaction solution, the drainage branch flow channel for discharging waste liquid, the first outlet and the second outlet, recovery branch flow channel and drainage branch flow channel are connected with the first outlet and the second outlet respectively, and are all connected with micro-reaction functional area by drainage flow channel.The present invention can automatically complete the sequential release of multiple reagents on chip, and flexibly control the flow rate and direction of microfluid.

A cell for a microfluidic analysis system is described wherein the cell is adapted to be traversed by a flow of a reagent fluid and comprising a hollow main container having an outer side wall defining an inner chamber, a lower base having a flow port and a well formation, a lower outlet port for inlet of the reagent fluids, a vertical outlet port for outlet of the reagent fluids from the cell with a lower inlet port arranged in the inner chamber adjacent to an upper edge of the well formation and an upper portion comprising an upper inlet port and an upper outlet port.

The application belongs to the technical field of urea nitrogen detection, and particularly relates to an integrated microfluidic chip device and method for detecting urea nitrogen; wherein a cover plate is bonded to a main plate, and the main plate is bonded to a base plate; the main plate is provided with a light path channel, a micro flow channel, a reaction chamber, a sample chamber, a control chamber and a groove; a photoelectric detector is fixed in the groove, an LED light source, a lens, a spectroscope and a reflecting mirror are fixed in the light path channel, the sample chamber and the reaction chamber are communicated through the micro flow channel, and the sample chamber and the outside are communicated through the micro flow channel; the cover plate is etched with an injection port communicated with the reaction chamber and the control chamber; the application adopts the microfluidic analysis chip idea, and through microfabrication technology, functional elements such as the LED light source, the lens, the spectroscope, the reflecting mirror, the reaction chamber, the sample chamber, the control chamber, the micro flow channel, the photoelectric detector and the main control chip are integrated on the chip like integrated circuits, and the idea of transferring the functions of the whole laboratory to the chip is concentratedly embodied.

The application discloses a kind of microfluidic titration device and titration method, it is related to microfluidic analysis detection technical field, including base, white background plate, black background plate, vertical column, horizontal beam, test tube clamp, titration test tube, background light box, background lamp, light shield, base level, adjustable foot, beam horizontal adjusting mechanism and beam micro level, automatic control unit, microfluidic flow control module, titration end point image recognition module, background driving module, data processing storage module;The bottom of base is provided with adjustable foot, base level is arranged on base, vertical column is fixed on base, horizontal beam is connected with vertical column by beam horizontal adjusting mechanism, beam micro level is fixed on horizontal beam, and test tube clamp is arranged on horizontal beam.A kind of microfluidic titration device and titration method, through the double leveling structure of base and beam, add automatic control module to realize intelligent regulation and control of whole process, eliminate benchtop and assembly error, and the batch detection efficiency of multi-channel clamping is improved.

The invention provides an edible mushroom heavy metal detection system, method and device, and relates to the technical field of food safety detection.The system comprises a detection cassette used for positioning a paper-based microfluidic analysis card and a smart phone slot; the paper-based micro-fluidic analysis card is provided with a filter layer, a solid-phase reagent release layer and a detection array layer which are sequentially arranged along the liquid permeation direction; a detection application program is installed on the smart phone; the detection method comprises the following steps: automatically completing filtration, reagent mixing and chromogenic reaction of a bacterial sample extracting solution through the analysis card, carrying out photographing and image analysis on a detection array area of the analysis card by using the smart phone arranged in the cassette, and calculating the heavy metal concentration; by combining the low-cost consumables with the smart phone, rapid, simple and low-cost field quantitative detection of the heavy metals in the edible mushrooms is realized, the operation is convenient, and the result is reliable.

The invention belongs to the technical field of paper-based microfluidic analysis, and particularly relates to a composite CaCO3-pullulan membrane as well as a preparation method and application thereof. The composite CaCO3-Pulullan film is formed by divalent calcium ions and Pulullan, the divalent calcium ions and the Pulullan can generate chelation reaction, the composite CaCO3-Pulullan film is a uniform opaque milk white film, and the thickness of the composite CaCO3-Pulullan film is 60-100 [mu] m. The composite CaCO3-Pulullan membrane is prepared by taking a Pulullan solution, calcium carbonate and polyvinyl alcohol mixed solution as precursors, has toughness and folding resistance, has the characteristics of flow control, timed flow closing and the like when being embedded into a paper-based microfluid device, and can realize control on flow rates of complexing solutions with different concentrations.

The utility model belongs to the technical field of kits, and particularly relates to a microfluidic analysis kit, which comprises an outer shell, a plurality of groups of first springs are arranged on the inner bottom surface of the outer shell, an inner shell is arranged at the top ends of the plurality of groups of first springs, a vibrating motor acts, and reagents in the inner shell are shaken, so that the reagents can be uniformly mixed, and the detection accuracy is improved. Insertion grooves are formed in the two sides in the inner shell, partition plates are arranged in the insertion grooves, insertion blocks are arranged at the two ends of each partition plate, the partition plates are easy, convenient and rapid to assemble and disassemble, the internal structure and layout of the kit can be flexibly adjusted according to experiment requirements, and the kit is convenient to use and popularize. Two sets of sliding strips are arranged at the bottom end of the shell, two sets of sliding grooves matched with the sliding strips are formed in the top face of the shell, and a plurality of kits are assembled and can be combined into a whole to be transported.

The disclosure features methods and systems for processing samples that include introducing a sample featuring one or more components in a first electrolyte solution into a separation channel of a fluidic device, applying an electrical potential difference across the sample to cause migration of at least one sample component toward an end of the separation channel, and adjusting at least one of a gas pressure in a reservoir comprising a second electrolyte solution, and a gas pressure external to an aperture positioned at the end of the separation channel, so that the gas pressure in the reservoir is greater than the gas pressure external to the aperture, and directing a flow of the second electrolyte solution in response to the gas pressures through the pumping channel and out of the aperture to discharge the at least one sample component through the aperture.

Fluid analysis system comprising: a handset that includes: a fluid analysis control module, wherein the fluid analysis control module is configured to initiate the taking of a sample of a fluid and to provide the sample to a microfluidic analyzer for performing a microfluidic analysis; and a telematics device connected to the fluid analysis control module and configured with a wireless transceiver; a management system located remotely from the telematics device, the management system being configured to wirelessly receive microfluidic analysis results transmitted from the telematics device; and an analysis controller connected to the fluid analysis control module and configured to initiate a first type of analysis of the sample in response to the occurrence of an analysis trigger and a second type of analysis of the sample in response to the results of the first type of analysis, wherein the first and second types of analysis are different; wherein the analysis trigger is selected from the group consisting of: altitude of the handset and geographic location of the handset as determined by a global navigation satellite system receiver.

The invention discloses a surface acoustic wave micro-fluidic chip and a cell compression ratio measuring method, and belongs to the technical field of micro-fluidic analysis. The at least two interdigital transducers are oppositely arranged and are parallel to each other; the micro-channel is fixed on the surface of the piezoelectric substrate and is positioned between the two interdigital transducers; the finger strips of the two interdigital transducers and the central axis of the micro-channel are obliquely arranged at a certain included angle; the surface acoustic wave generated by the interdigital transducer is propagated to the fluid in the micro-channel along the piezoelectric substrate to form a surface acoustic wave standing wave field; the surface acoustic wave standing wave field and the flow field in the micro-channel change the movement mode of the cell by adjusting the Stokes drag force and the acoustic radiation force borne by the cell so as to measure the cell compression ratio. Aiming at the limitation of an inclination angle surface acoustic wave standing wave micro-fluidic chip in measurement of mechanical properties of cells in the prior art, accurate regulation and control are realized.

The present invention discloses a programmable paper chip and a preparation method thereof, and a microfluidic analysis chip, which belongs to the field of paper-based microfluidic technology. The programmable chip is a laminated structure and includes a lower film layer, a paper layer, and an upper film layer from bottom to top; there is adhesion between the lower film layer and the upper film layer and the paper layer; the paper layer includes: a paper sheet made of a paper-based material; a flow channel boundary drawn with oil-based ink is provided on the paper sheet, and the area within the flow channel boundary is a fluid channel; the fluid channel is separated by a timing valve, and the timing valve is an area impregnated with water-based ink; a sample addition hole connected to the fluid channel on one side of the timing valve is provided on the upper film layer; wherein the oil-based ink and the water-based ink are both hydrophobic inks. The preparation of the timing valve specifically includes: pre-cutting the boundary of the preset area and then impregnating it with water-based ink to a saturated state. The present invention can accurately, quickly, and low-costly achieve fluid control without introducing substances outside the system and without relying on external large-scale equipment.

A method for assigning at least one specific reagent to a reaction space of a carrier device for the processing of a substance within a microfluidic analysis system includes: reading in a spatial pattern arrangement of the reaction spaces on the carrier device, at least a first reaction space assigned a first specific reagent and a second reaction space assigned a second specific reagent; determining a starting reaction space to which a specific reagent has not yet been assigned; measuring a first and second spatial distances, respectively, between the starting reaction space and the first and second reaction spaces; and defining the first specific reagent as the specific reagent of the starting reaction space if the first distance is greater than the second distance and / or defining the second specific reagent as the specific reagent of the starting reaction space if the second distance is greater than the first distance.

The application relates to the technical field of microfluidics, and discloses a modular tubular microfluidic analysis and detection device and a detection method, which comprises the following steps: manufacturing a fluid input module and a reaction and measurement module by using laser cutting, machining, injection molding or 3D printing technology; the fluid input module comprises a plurality of fluid input mechanisms; the fluid input mechanism comprises a liquid storage device, a first micropump and a first microvalve, and each fluid input mechanism is independent of each other; the reaction and measurement module comprises a microreactor and a signal reader; the signal reader is connected with the microreactor; a hose is used to connect the fluid input module and the microreactor; the liquid to be detected is stored in the liquid storage device; the liquid to be detected flows into the microreactor through the hose under the control of the first micropump and the first microvalve; the signal reader acquires signal changes when the liquid to be detected in the microreactor reacts; and analysis and detection results are obtained according to the signal changes. The application can realize a stable pipeline structure, facilitate function expansion and reduce costs.

The invention relates generally to biochemical assays and more particularly to digital microfluidics multi-dynamic range parallel biochemical assays. The invention provides methods that enable improved precision and linearity for digital microfluidics analyses (i.e., analyses performed on a droplet actuator using droplet operations), including for example, assays related to measuring analytes in a biological sample. Examples of assays that may be performed using a method of the invention include measuring analytes in blood, including analytes from blood components, such as analytes present in plasma. The invention provides a digital microfluidics device (or cartridge) and methods for performing a biochemical assay using the microfluidics device to measure an analyte in a sample.

The invention relates to a substrate (100) for the uptake and microfluidic analysis, in particular counting, of biological cells (40), especially circulating tumor cells (40), wherein the substrate (100) has cavities (120) on a top surface (110) for receiving the cells (40), characterized in that the substrate (100) comprises a textile fabric (160), wherein the textile fabric (160) is fluidically connected to at least some of the cavities (120). The invention further relates to a microfluidic device (200), and a method (500) for the uptake and microfluidic analysis of biological cells (40) with such a substrate (100), as well as a manufacturing method (600) of such a substrate (100).

This application provides a microfluidic analysis device, including a driving device, a detection device, and a control device. The control device is used to: control the driving device to rotate a microfluidic disk so that a diluted sample flows into the colorimetric well of the microfluidic disk; control the driving device to rotate the microfluidic disk to mix the sample in the colorimetric well to obtain a detection solution; and acquire detection data of the detection solution from the detection device, and determine the detection result corresponding to the detection data. Specifically, between the flow of the diluted sample into the colorimetric well and the activation of the detection device to detect the detection solution, the driving device drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well. This prevents air bubbles in the colorimetric well from interfering with the accuracy of the detection data of the detection liquid, thereby improving the accuracy of the sample detection results.

The invention belongs to the technical field of microfluidic analysis and detection, and particularly relates to a living cell sampling and detecting device, method and system. The device comprises a rotary micro-fluidic chip and an amplification detection reagent, the rotary micro-fluidic chip is formed by sequentially assembling an upper electrode, a nanopore film, a sampling layer, a lower electrode and a detection layer from top to bottom; the rotary micro-fluidic chip is of a detachable structure, and the structures can be connected through a central bearing; the nanopore membrane is arranged in the electric sampling area of the sampling layer and is used for continuously sampling living cells; a gene detection area is arranged on the detection layer, and an amplification detection reagent is placed in the gene detection area and is used for detecting the expression quantity of genes in cells. The invention provides a method capable of continuously sampling genes in living cells and accurately quantifying the genes in a simple environment.

A centrifugal microfluidic analysis chip comprises a disc-structured substrate and a chip body which consists of cover plates bonded on the upper surface and the lower surface of the substrate; a rotation positioning hole which is provided in a circle center area of the chip body and connected to a rotating shaft; a sample inlet hole, an exhaust hole and an exhaust channel which are provided on the substrate, wherein the sample inlet hole can be cylindrical or conical so as to realize a sample injection operation, and the exhaust hole is used for maintaining constant air pressure in the channel in a sample addition process; and multiple liquid equipartition and detection units which are arranged at intervals in the circumferential direction around the circle center of the chip body according to a set radius and are sequentially communicated to form a liquid equipartition detection system. The present invention aims to solve the problems that existing chip bodies have complex structures, centrifugal operations require multiple rotational speeds, liquid dispensing is inaccurate, detection flux is low under a given chip body size, and supporting instruments have high costs.

The invention relates to the field of physiological status detection, and provides a three-dimensional paper-based microfluidic electrochemical analysis device for sweat in-situ monitoring. The device is characterized in that the three-dimensional paper-based microfluidic network and the electrochemical sensing array are integrated, so that various substances in human sweat can be monitored at the same time, and real-time and accurate physiological state information can be provided. The core of the invention lies in that a layer-by-layer manufacturing method is adopted to realize direct preparation of the three-dimensional paper-based microfluidic network, and the three-dimensional paper-based microfluidic network has high customizability. The polydimethylsiloxane is used as a key material, not only serves as a hydrophobic barrier of the microchannel to effectively prevent fluid leakage, but also serves as an adhesive to enhance the overall structural stability of the device. In addition, the flexible electrochemical sensing array is embedded into the three-dimensional paper-based microfluidic network, so that synchronous monitoring of various substances in sweat is realized. The invention provides a new method for manufacturing the three-dimensional paper-based microfluidic analysis device, and has a wide application prospect in the fields of personalized diagnosis and physiological monitoring.