38 results about "Colocalization" patented technology

A novel optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described. Two unique families of fluorophores are used, namely energy-transfer fluorescent beads and semiconductor nanocrystal (NC) quantum dots, that can be excited by a single laser wavelength but emit at different wavelengths. A novel multicolor sample-scanning confocal microscope was constructed which allows one to image each fluorescent light emitter, free of chromatic aberrations, by scanning the sample with nanometer scale steps using a piezo-scanner. The resulting spots are accurately localized by fitting them to the known shape of the excitation point-spread-function of the microscope.

The present invention provides methods for detecting a target pathogenic agent, e.g., a virus, a bacterium, and/or a toxic substance, using colocalization detection. The invention also provides methods for parallel detection of different target pathogenic agents in a sample using multiplexed labeling and colocalization detection. The invention also provides kits comprising sets of probes for detecting pathogenic agents. The invention further provides computer systems and computer program products for carrying out the method of determining degrees of colocalization.

Methods of diagnosing whether an epithelial tissue is an abnormal tissue by determining an expression pattern for PML in the epithelial tissue; determining an expression pattern for nuclear bodies in the epithelial tissue; determining SUMO-1 colocalization and comparing the expression pattern for PML and the expression pattern for nuclear bodies with a control are disclosed. Also disclosed are methods for diagnosing whether a subject has mild dysplasia, moderate dysplasia, Type A severe dysplasia, Type B severe dysplasia, cervical squamous cell carcinoma, or poorly-differentiated cervical squamous cell carcinoma, by determining an expression pattern for PML, an expression pattern for nuclear bodies, and SUMO-1 colocalization and determining whether the sample is consistent with expression patterns expected for mild dysplasia, moderate dysplasia, Type A severe dysplasia, Type B severe dysplasia, cervical squamous cell carcinoma, or poorly-differentiated cervical squamous cell carcinoma.

The invention discloses a rhodamine B targeted lysosome pH fluorescent probe with a cysteine ethyl ester structure, wherein the rhodamine B targeted lysosome pH fluorescent probe has the structure as shown in a formula (I). Meanwhile, the invention discloses an application of the probe as a living cell lysosome pH fluorescent probe. Experiments show that the probe provided by the invention does not generate fluorescence under the neutral and alkaline conditions, the fluorescence intensity is rapidly enhanced along with the reduction of the pH value of the solution, is up to the maximum value when the pH value is about 4.0 and is enhanced by about 150 times when the pH value ranges from 7.51 to 3.53, and the probe has favorable antijamming capability and reversibility in the presence of various metal ions. An intracellular colocalization experiment and an interlysosome pH regulation experiment prove that the probe can specially mark a lysosome and sensitively monitor the small change of the interlysosome pH value. A cell survival rate experiment shows that the probe is nontoxic to cells, which indicates that the probe disclosed by the invention has an important application value in the aspects of imaging the cells and monitoring the change of the interlysosome pH value.

Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.

The invention provides a data transmission control method, a network side device and a terminal, wherein the method comprises the following steps: the network side device maps code words on at least one data layer; the network side device maps the data layer on at least one quasi colocalization QCL group, wherein the QCL group is an antenna port group established according to a QCL relation amongvarious antenna ports; and the network side device performs data transmission based on the QCL group. With the method, the device and the terminal provided by the invention, data transmission of a multi-array antenna is realized.

The invention provides a fluorescent probe for detecting the viscosity. The chemical structural formula of the fluorescent probe is shown in the description. The fluorescent probe can be used for detecting the viscosity of a solution or a cell. The donor of the probe is an imidazolyl group, the acceptor of the probe is a hemicyanine group, and the donor and the acceptor are combined through a phenyl ring. The probe has the advantages of short synthesis steps, and simplicity in post-treatment; the probe can realize precise location of mitochondria, and has a high colocalization coefficient; andthe enhancement of the fluorescence color can be observed under an ultraviolet lamp, so the fluorescent probe has a chromophoric sensing function. The probe of the invention is a simple and sensitiveaccurate-location viscosity specific detection reagent, and has a broad application prospect in the field of biomolecule detection.

The present invention provides a novel method called Sequential IMmunoPeroxidase Labeling and Erasing (SIMPLE) that enables the simultaneous visualization of at least five markers within a single tissue section. Utilizing the alcohol-soluble peroxidase substrate 3-amino-9-ethylcarbazole (AEC), combined with a rapid non-destructive method for antibody-antigen dissociation, the present application discloses the ability to erase the results of a single immunohistochemical stain while preserving tissue antigenicity for repeated rounds of labeling. The present invention also provides methods for visualizing multiple antigens simultaneously.

The present invention provides methods for utilizing a form of optimized suspension culture to examine the infectivity of pathogenic organisms and agents in human cells and tissues. Also provided are methods using a rotating wall vessel to predict chemosensitivity of cells and tissues to toxins and chemotherapeutic agents. These culture conditions potentiate spatial colocalization and three-dimensional assembly of individual cells into large aggregates which more closely resemble the in vivo tissue equivalent. In this environment, dissociated cells can assemble and differentiate into macroscopic tissue aggregates several millimeters in size. These culture conditions allow for better cellular differentiation and formation of three-dimensional cellular aggregates, more efficient cell-to-cell interactions, the in in vivo-like exchange of growth factors and greater molecular scaffolding facilitating mechanical stability for cells. The suspension culture system offers a new approach for studying microbial infectivity from the perspective of the host-pathogen interaction and also for analyzing chemosensitivity to toxins and chemotherapeutic agents.

The invention discloses a colocalization trigger chain type hybridization reaction based aptamer sensor for detecting adenosine. The colocalization trigger chain type hybridization reaction based aptamer sensor consists of an aptamer chain 1, an aptamer chain 2 and a hairpin probe which are biotinylated and a magnetic nano-sphere modified by streptavidin. The aptamer sensor is used for detecting adenosine, and a detection method comprises the following steps: (1) activation of a streptavidin modified magnetic sphere; (2) fixation of the biotinylated aptamer chain 1; (3) colocalization of adenosine-induced double aptamers; (4) colocalization trigger chain type hybridization reaction; (5) addition of a dye SYBR Green I and fluorescence detection; (6) calculation: calculating the concentration of adenosine in a to-be-detected object containing adenosine according to a measured fluorescence value. The aptamer sensor disclosed by the invention can be used for reducing the non-specific reaction of conventional chain type hybridization reaction by virtue of the colocalization-induced hybridization reaction, has the advantages of high detection sensitivity and less sample usage without enzyme amplification, and can achieve the analysis and detection of low-concentration adenosine.

The invention discloses a bimodal microscopic imaging system and an imaging method thereof. According to the bimodal microscopic imaging system combining two-photon fluorescence and optical diffraction tomography, synchronous control is achieved through the control subsystem, and the problems encountered by two-photon fluorescence imaging are solved by means of the characteristics of no mark, no invasion and small phototoxicity of optical diffraction tomography; meanwhile, a result in diffraction chromatography is calibrated by utilizing two-photon fluorescence, so that a biological sample is imaged from morphological and chemical specificity, and the two-photon fluorescence imaging device is simple and can realize relatively high resolution without a complicated imaging light path; the two modalities are fused based on the control subsystem, and parallel imaging characterization of local specificity and global morphology of the sample is achieved; and according to the invention, the three-dimensional high-resolution refractive index distribution of the to-be-detected sample can be recovered through optical diffraction tomography, and co-positioning is carried out on the two-photon fluorescence imaging of the to-be-detected sample at the same time, so that bimodal microscopic imaging is realized.

The invention discloses a mitochondria targeted membrane-permeable cyclopeptide as well as a preparation method and a use thereof, wherein the structure of a mitochondria targeted peptide is as follows: the mitochondria targeted membrane-permeable cyclopeptide can be specific to targeting cell mitochondria, and a confocal inverted fluorescence microscopic imaging experiment shows that the structure has good membrane permeability to Hela cells, and the colocalization coefficient Pr of the structure and a commercial mitochondrial dye is equal to 0.74, and the structure can be efficiently targeted and localized to mitochondria.

The invention discloses a ribosome rRNA two-photon fluorescent probe-quaternary ammonium salt terpyridine derivative as well as a preparation method and application thereof. The structural formula of the ribosome rRNA two-photon fluorescent probe-quaternary ammonium salt terpyridine derivative is as shown in the description. A target product disclosed by the invention can identify RNA by two-photon fluorescent enhancement and realizes a two-photon fluorescent enhancement identification effect; and low-energy long wave (710 nm) excitation has little damage to a living body. The rRNA in cell nucleolus is specifically identified. The colocalization effect of the target product disclosed by the invention and nucleolus Syto9 at the cell nucleolus is good, and a Pearson coefficient can reach 90 percent.

A computer-implemented method and a system are provided for visualising colocalised fluorescence signals. The method accesses signal intensity data obtained from a first fluorescence channel and a second fluorescence channel in which the signal intensity data is associated with voxels in an image. A regression factor on the signal intensity data is calculated to generate a regression parameter corresponding to a degree of correlation between the signal intensity data obtained from the first and second fluorescence channels The signal intensity data is mapped to the regression parameter and colourmap values are assigned to each voxel based on the mapped signal intensity data in which colourmap values of voxels embodying poorly correlated signal intensity data are reduced. The method renders the voxels in the image in colours according to their colourmap values to visualise colocalisation in the image.

The invention discloses a super-resolution imaging probe based on silicon dioxide and a preparation method and application of the probe.The super-resolution imaging probe includes silicon dioxide nanoparticles connected with an Alexa Fluor 647 dye, and the surface of the super-resolution imaging probe is coupled with an HER2 (human epidermal growth factor receptor-2) nucleic acid aptamer; the probe can be used for targeted recognition of HER2 overexpression cell exosomes, that is, the HER2 overexpression cell exosomes are subjected to fluorescence labeling; super-resolution optical imaging canbe carried out on the cell exosome which specifically recognizes the probe by utilizing an ultra-high resolution microscope; the probe is uniform in particle size, small in size, high in positioningprecision, excellent in scintillation performance and suitable for ultrahigh-resolution optical imaging based on a single-molecule positioning method; and the exosome is marked by the super-resolutionimaging probe, and the exosome of the cell can be accurately marked by using a single-molecule positioning microscope in a double-channel co-positioning mode.

The invention discloses an MDA-MB-231 exosome detection method based on two-color co-localization and application. The detection method comprises the following steps: marking an MDA-MB-231 cell exosome through a cell membrane probe CM-DiI; meanwhile, a PD-L1 nucleic acid aptamer probe with another fluorophore FAM is used, and the probe can specifically capture exosomes secreted by MDA-MB-231 cells highly expressed by PD-L1; the captured exosome can be subjected to fluorescence imaging through a total internal reflection (TIRF) imaging technology; and by combining a two-color fluorescence co-localization technology, a false positive event can be judged on a single particle level, and the accuracy of an exosome fluorescence immunoassay technology is improved.

The present invention generally relates to imaging cells, for example, to determine phenotypes and/or genotypes in populations of cells, e.g., to build genotype-phenotype corresponse for high-throughput screening. In some cases, the cells may be manipulated, e.g., using CRISPR or other techniques. In certain embodiments, nucleic acids may be introduced to the cell, e.g., using a lentivirus. The nucleic acids may contain a guide portion comprising a DNA or RNA recognition sequence, a reporter portion, and an identification portion comprising one or more read sequences. The guide portion may be used to alter the phenotype of the cells, e.g., using a sequence, e.g., an sgRNA sequence, that can be targeted using CRISPR or other techniques, and in some cases, the phenotype of the cells may be determined using various imaging approaches. The identification portion may be determined using MERFISH or other suitable techniques. In addition, in some cases, association or colocalization between determination of the reporter and the read sequences may substantially improve decoding accuracy, e.g., due to lowered misidentification of background signals. Other aspects are generally directed to compositions or devices for use in such methods, kits for use in such methods, or the like.

The present invention relates to the use of one or more biomarkers to evaluate the likelihood that a CDK4 inhibitor would produce an anti-cancer effect in a subject. Accordingly, in certain non-limiting embodiments, the present invention provides for methods, compositions and kits for a companion diagnostic for CDK4 inhibitors, and in particular, for the use of the colocalization of Enigma and CDH18 biomarkers to foci within the cancer for determining whether the cancer can be successfully treated by CDK4 inhibition.