284 results about "Tetramer" patented technology

The invention describes a process for tetramerisation of olefins wherein the product stream of the process contains more than 30% of the tetramer olefin. The process includes the step of contacting an olefinic feedstream with a catalyst system containing a transition metal compound and a heteroatomic ligand.

Provided herein is an oligomerization product formed from alpha-olefins having at least three carbon atoms comprising dimers, at least about 80 weight percent of which are linear. In an embodiment, an oligomerization product formed from alpha-olefins having at least three carbon atoms comprises trimers, at least about 20 weight percent of which are linear. In another embodiment, an oligomerization product formed from alpha-olefins having at least three carbon atoms comprises tetramers, at least about 5 weight percent of which are linear. In yet another embodiment, an oligomerization product formed from alpha-olefins having at least three carbon atoms comprises pentamers, at least about 5 weight percent of which are linear.

The invention relates to variant peptides which bind to HLA molecules, leading to lysis of cells via cytolytic T cell lines. The variants are based upon NY-ESO-1 peptides. The peptides can be incorporated into immune tetramers, which are useful as T cell sorters.

The invention relates to variant peptides which bind to HLA molecules, leading to lysis of cells via cytolytic T cell lines. The variants are based upon NY-ESO-1 peptides. The peptides can be incorporated into immune tetramers, which are useful as T cell sorters.

Solution-based methods for identifying an MHC-binding peptide or measuring affinity of MHC-binding peptides for an MHC monomer, or modified MHC monomer by incubating at least one MHC monomer or modified MHC monomer having a bound template MHC-binding peptide, an excess amount of a competitor peptide, and a tracer MHC-binding peptide tagged with a detectable label so as to allow competition binding between the three peptides At least a portion of the competitor peptide exchanges with the template peptide and a difference in signal produced by the detectable label in the total sample as compared with signal produced solely by monomers after the competition assay is obtained and used to calculate affinity of the competitor peptide for the monomer. These methods are useful in peptide discovery programs and exchanged monomers can be further tested for activity in tetramer cell staining assays.

The present invention relates to one kind of protocyanidin oligomer and its production process and application in food, health article, feed additive and animal medicine. The protocyanidin oligomer is dimer, trimer and tetramer, and is produced with condensated tannin as material and through oxidizing degradation with hydrogen peroxide or potassium chlorate as oxidant to obtain yellow or brown semi-transparent liquid and subsequent separation and purification. The protocyanidin oligomer of the present invention has powerful antioxidant function and antibiotic function, and my be used in food owing to no astringent taste and no negative effect on the activity of amylase in digestive tract, as feed additive to reduce coccidiosis infection of fowls and low cholesterol content in egg.

A bridging assay that utilizes a multivalent MHC binding molecule to enumerate the number of antigen-specific CTLs in a particular sample and also determines the functional capability of the CTL population in the sample is provided. In one embodiment, the assay is used to measure the effector function of any tetramer-positive CTL using a single non-MHC-containing target cell line that is adapted to form an antibody bridge with the tetramer. Furthermore, effector function and enumeration can be measured by flow cytometry, and additional markers residing on either effector or target cell populations may be detected using antibodies coupled with other fluorochromes. The tetramer bridging assay will allow investigators to easily determine the lytic capacity and antigenic specificity of CTLs using a commercially available reagent in a non-radioactive assay.

Endotoxin, also known as lipopolysaccharides (LPS), is the major mediator of septic shock due to Gram-negative bacterial infection. Chemically synthesized S3 peptide, derived from Sushi3 domain of Factor C, which is the endotoxin-sensitive serine protease of the limulus coagulation cascade, binds and neutralizes LPS activity. Fluorescent tagged-S3 is shown to detect LPS-containing bacteria. For large-scale production of S3 and to mimic other pathogen-recognizing molecules, tandem multimers of the S3 gene were constructed and expressed in E. coli. Tetramer of S3 for example is shown to display an enhanced inhibitory effect on LPS-induced activities. An affinity matrix based on tetramer of S3 is also shown to be particularly efficient at removing LPS.

Analogs of ShK toxin and methods for using such ShK analogs. The ShK analogs generally comprise ShK toxin attached to a chemical entity (e.g. an atom, molecule, group, residue, compound, moiety, etc.) that has an anionic charge. In some embodiments the chemical entity attached to the ShK toxin may comprise an amino acid residue. The ShK analogs may be administered to human or non-human animal subjects to cause inhibition of potassium channels or to otherwise treat diseases or disorders. In some embodiments, the chemical entity to which the ShK toxin is attached may be chosen to provide selective inhibition of certain potassium channels (e.g., Kv1.3 channels) over other potassium channels (e.g., Kv1.1 channels). In come embodiments, the chemical entity to which the ShK toxin is attached may include a fluorophore, thereby providing a fluorophore tagged ShK analog. Such fluorophore tagged ShK analogs may be used in flow cytometry alone, or in conjunction with class II tetramers that can detect autoreactive cells.

New protein coated surfaces, which have a high capacity for capturing target molecules, thus yielding assays with enhanced sensitivity, are disclosed. Surfaces prepared according to the present invention contain a coating consisting essentially of streptavidin, avidin or "NeutrAvidin" in polymeric form, wherein polymerization has been controlled to an extent such that the polymer is predominantly dimers, trimers and tetramers of the native molecule.

Fluoroelastomers are disclosed which have a glass transition temperature less than −10° C. The elastomers contain copolymerized units of a perfluorovinylpolyether that is derived from a trimer or tetramer of hexafluoropropylene oxide.

The invention relates to variant peptides which bind to HLA molecules, leading to lysis of cells via cytolytic T cell lines. The variants are based upon NY-ESO-1 peptides. The peptides can be incorporated into immune tetramers, which are useful as T cell sorters.

The invention belongs to the field of biotechnology development and application research, and discloses a preparation method for an HLA-A0201 limited antigen specificity CTL (cytotoxic T lymphocyte). The method comprises the following steps: collecting eripheral mononuclear cells one by one, so as to enrich and purify a CD8+T lymphocyte; stimulating the CD8+T lymphocyte with a mature dendritic cell bearing an HLA-A0201 limited target antigen polypeptide, and promoting the growth of the T lymphocyte through the combination of rhIL-2 and rhIL-7; purifying the target CTL according to a Tetramer marking method and a flow cell sorting method; stimulating the growth of the target CTL according to a solid phase coated anti-human-CD3mAb and IL-2; adding an autologous PBMC (peripheral blood mononuclear cell) to enhance the actification of the target CTL; and adding rhIL-15 for enlarging cultivation, collecting and identifying. The CTL prepared according to the method has the advantage that the purity, the proliferation capability, the cytotoxicity and the CTL-CM proportion are high, so as to be used for immunological therapy of tumors and the like.

The present disclosure is in relation to antiviral preparations obtained from plant sources namely Cinnamon, Litchi and Arachis. It provides a composition and a process to prepare the composition comprising pentameric procyanidin flavonoid, trimers and tetramers. The composition improves immunity response and found to be useful in treatment and management of HIV infection and AIDS and for the prevention, treatment and management of Influenza virus and infection.

The present invention provides a multimeric form of a Tie 2 binding peptide monomer, wherein the multimeric form has Tie 2 agonist activity. The multimeric form, preferably a tetramer, stimulates angiogenesis and promotes wound healing. The present invention also features pharmaceutical compositions comprising the multimeric Tie 2 agonists, including those suitable for topical or systemic administration. Methods of using the multimeric Tie 2 agonists of the invention for stimulating angiogenesis and for promoting healing of wounds, such as diabetic ulcers or skin grafts, are also provided.

The invention discloses a method for in vitro induction of specific cytotoxic T lymphocytes of hepatitis B virus (HBV). The method comprises the following steps of: 1, preparing artificial antigen presenting cells (aAPC) capable of loading any polypeptides; 2, loading aAPC on epitope peptides; and 3, loading aAPC in vitro induction HBV antigen specific CTL of antigen peptides; measuring the number of obtained antigen specific CTL by a Tetramer decoration method, namely combining streptavidin marked by fluorescein and four MHC-peptide compounds marked by biotin to form the Tetramer, namely MHC-peptide tetramer; and after the MHC-peptide tetramer is combined with TCR on the antigen specific CTL, measuring by a flow cytometry. The method can be applied to the amplification of T lymphocytes resisting the HBV.

The invention provides a qualitative and quantitive isothermal detection method of RNA (Ribonucleic Acid). The method comprises the steps of after cutting RNA at fixed points by dnazyme (DNAzyme), amplifying RNA through a strand displacement isothermal amplification technique (SDA); and carrying out qualitative or quantitive detection by inspecting a reporter group G-tetramer released from an SDA product. The method can measure trace RNA (comprising mRNA and miRNA) quickly, simply, conveniently, highly sensitively and specifically, and meanwhile, the risk of pollution is reduced.

The present invention discloses a 17beta-estradiol visualization detection method based on DNA nano-structure, and a 17beta-estradiol visualization detection kit based on DNA nano-structure. The working principle is that 17b-estradiol interacts with aptamer, a strand displacement reaction is started, three groups of stem-loop DNA probes are constantly opened to form DNA nano-structures, a G tetramer having catalysis activity is formed on the terminals of the three DNA arms in the DNA nano-structures, the G tetramer is bound with hemin so as to form a compound having catalysis activity and similar to HRP, TMB is subjected to catalytic oxidation, and a blue substrate is produced, wherein the result is visible, and the concentration of 17b-estradiol is directly related to the blue shade. According to the present invention, the operation is simple, the whole reaction can be completed at the room temperature, the high sensitivity is provided, the detection limit on 17b-estradiol is 100 fM, the good specificity is provided, the detection result is directly visible without any detection equipment, and the method and the kit can be used for the on-site rapid detection of 17b-estradiol.

The present invention provides aniline oligomers, their aliphatic polyester copolymer and their preparation. Two kinds of aniline oligomers are first synthesized with N-phenyl-1, 4-p-phenylene diamine as material, and then copolymerized with aliphatic polyester to obtain electrically active biodegradable polymers. During the preparation, N-phenyl-1, 4-p-phenylene diamine has its end amido group protected with butanedioic anhydride and is then reacted with end amido aniline dimer and phenylene diamine to obtain aniline tetramer with one end carboxyl group and one end amino group and aniline pentamer with two end carboxyl groups; and the aniline oligomers are finally polycondensated with double hydroxyl group terminated aliphatic polyester to obtain the copolymers containing electrically active aniline oligomer block. The copolymers possess the advantages of both aniline oligomer and aliphatic polyester, and is used as biomedicine material mainly.