25 results about "Tetramer" patented technology

The invention is applicable to the technical field of metal organic framework materials, and provides a pillararene-based metal organic framework material and a preparation method thereof.The pillararene-based metal organic framework material takes a compound PY2-P5 as a ligand, and the material is one of PMOF1, PMOF2 and PMOF3; the PMOF1 is of a Cu4I4 cubic tetramer structure; the PMOF2 is of a Cu4I4 ladder tetramer structure, and the Cu4I4 ladder tetramer structure is a And the PMOF3 has a CuI one-dimensional chain structure. By introducing the pillararene ligand and regulating and controlling the coordination mode of the pillararene ligand and cuprous iodide, the metal organic framework material with stable structure and functional characteristics is constructed, the metal organic framework material has the dual advantages of host recognition site and fluorescence signal response, and can selectively distinguish 1-bromoalkane and 2-bromoalkane; a remarkable fluorescent blue shift signal is shown in the adsorption process, so that the visualization of molecular recognition and sensing is realized; the excellent molecular recognition capability of pillararene is maintained, the stability and reusability of the material are improved through framing, and a new way is provided for selective detection and separation of volatile organic compounds.

The present invention provides an application of a highly homogeneous monovalent streptavidin tetramer in the field of detection. Specifically, the present invention provides a detection system, and the detection system includes: (a) a multi-component complex having a structure shown in Formula I, (A-B)-(C-D m ) n (Formula I) Each component in the formula includes: A is a second binding protein that can specifically bind to a target analyte; B is the biotin; C is a monovalent streptavidin tetramer; D is a fluorescent label; "-" is a bond or a linking group; and, m is a real number between 1 and 6, and n is a real number between 2 and 8; (b) a first binding protein crosslinked to a solid-phase carrier Z0, and the first binding protein can specifically bind to the target analyte; (c) an optional target analyte; the first binding protein and the second binding protein can respectively bind to the target analyte, and there is no competition between the two. The detection system provided by the present invention can improve the stability of the detection reagent and ensure the sensitivity and accuracy of the detection.

The present invention relates to a composition, a kit and a detection method for detecting hydrogen peroxide on the basis of self-biotinylation according to the peroxidation activity of a guanine quadruplex, and, more specifically, to a composition, a kit and a detection method for detecting hydrogen peroxide, the composition comprising: i) a guanine quadruplex-hemin complex (G-quadruplex-hemin complex); ii) biotinyl tyramide; and iii) a probe capable of binding to the guanine quadruplex.

Provided is a method for improving the stability of urate oxidase. The inventors have discovered that combining an active ingredient urate oxidase or chemically modified urate oxidase with a stabilizer xanthine through a non-covalent bond can significantly improve the in vivo and in vitro stability of urate oxidase and chemically modified urate oxidase in the form of a tetramer, thereby effectively improving the stability of urate oxidase.

The application discloses a construction method of a UPLC characteristic spectrum of a radix-lithospermi and application thereof, 1) preparation of a control medicinal material reference solution; 2) preparation of a control reference solution: a certain amount of protocatechuic acid, protocatechuic aldehyde, ferulic acid, rosmarinic acid, caffeic acid tetramer and danshensu are taken to prepare a mixed solution by adding methanol, serving as the control reference solution; 3) preparation of a test solution; 4) determination: the control medicinal material reference solution, the control reference solution and the test solution are respectively taken and injected into a liquid chromatograph to determine characteristic spectra, wherein the characteristic spectra include at least 12 characteristic peaks; 5) qualitative analysis of a sample to be determined is carried out by using the characteristic spectra obtained in the above steps. The characteristic spectrum includes 12 characteristic peaks, can effectively distinguish radix-lithospermi (Xinjiang radix-lithospermi) and its pseudo products of different origins, and embodies the integrity of quality control of radix-lithospermi.

Inventors describe herein the functional and structural characteristics of the MGL from the anaerobic alkaliphilic thermophile Thermobrachium celere (TcMGL), which was compared to that from Pseudomonas putida (PpMGL). Catalysis was limited to L-Met, L-homocysteine, and L-cysteine, with the highest catalytic efficiency (keat / KM) towards L-Met. The half-life of active TcMGL in human plasma at 37°C was 9.2 hour. Half-life increased from 11.3 hour to 66.7 hour with increasing PLP concentration from 2 to 200 µM, in much greater magnitude than in PpMGL case. TcMGL crystal structure resolution revealed that the enzyme is a tetramer of two catalytic dimers containing a mobile region with open and closed conformations resulting in differences for access to the active site. TcMGL possesses favourable characteristics which makes it a suitable candidate for treatment of cancer. The present invention relates to a polypeptide comprising an amino acid sequence as set forth by SEQ ID NO: 1 or a function-conservative variant thereof.

The disclosure herein relates, inter alia, to inventive cucurbituril compounds and glycoluril compounds having formula (II), glycoluril compounds having formula (II'), glycoluril bisethers of formula (III), glycoluril dimers of formula (IV), glycoluril tetramer compounds of formula (V), and novel methods for their preparation using sodium 4,4'-(1,4-phenylenebis(oxy))bis(butane-1-sulfonate), which result in significantly improved purity and yields of intermediates and final products.

The invention discloses a construction method and application of a lithospermum erythrorhizon UPLC (Ultra Performance Liquid Chromatography) specific chromatogram. The construction method comprises the following steps: 1) preparing a reference substance solution of a reference medicinal material; 2) preparation of a reference substance solution: taking a protocatechuic acid reference substance, a protocatechuic aldehyde reference substance, a ferulic acid reference substance, a rosmarinic acid reference substance, a caffeic acid tetramer reference substance and a tanshinol reference substance with set amounts, and adding methanol to prepare a mixed solution as the reference substance solution of the reference substance; (3) preparing a test solution; 4) determination: respectively sucking the reference substance solution of the reference material of the Sinkiang lithospermum, the reference substance solution of the reference substance and the solution of the test article, and injecting the solutions into a liquid chromatograph for determination to obtain a characteristic spectrum which comprises at least 12 characteristic peaks; and 5) carrying out qualitative analysis on the sample to be detected according to the characteristic chromatogram obtained in the above step. The specific chromatogram provided by the invention has 12 characteristic peaks, can effectively distinguish lithospermum (Sinkiang lithospermum) and counterfeit products thereof with different bases, and embodies the integrity of quality control of lithospermum.

The invention relates to the technical field of functional materials, and discloses a preparation method of an iron-based metal complex and application of the iron-based metal complex in peroxidase-like activity, the chemical formula of the iron-based metal complex is [Fe2 (H4L4) (H2O) 4]. (H2O) 2, H4L4 is a deprotonated tetramer ligand, and the iron-based metal complex is prepared by taking FeSO4. 7H2O as a metal salt to react with the tetramer ligand. The iron-based metal complex disclosed by the invention is simple and convenient in synthesis process, high in purity and stable in structure, has excellent peroxidase-like activity, keeps catalytic performance in a wide range of pH value of 3-8, achieves the best activity when the pH value is 3, breaks through the dependence of most nano-enzymes on an acidic environment, and has a wide application prospect. And a catalytic material with wider adaptability is provided for biological catalysis and in-vitro detection.

The invention belongs to the technical field of optical metasurfaces, and particularly relates to a rotationally symmetrical lithium niobate elliptical nano-column quaii-BIC metasurface and a design method and application of the rotationally symmetrical lithium niobate elliptical nano-column quaii-BIC metasurface. The method comprises the following steps: 1, providing a cylindrical tetramer symmetric BIC metasurface, wherein the metasurface comprises a plurality of square lattices and incident light; 2, when the incident light is x polarization, the radius length of the single side in the cylindrical tetramer is increased or decreased at the same time, asymmetric disturbance is introduced, and the cylindrical tetramer is changed into a windmill type elliptical nano-column tetramer; the elliptical nano-column tetramer is composed of four elliptical nano-columns, the radiuses of the fixed sides of the four elliptical nano-columns are 250nm, the heights of the four elliptical nano-columns are 450nm, and the windmill type elliptical nano-column tetramer is made of lithium niobate. The intensity and waveform of a transmission spectrum can be flexibly modulated by adjusting related structure parameters of the metasurface, and the formed metasurface is relatively high in sensitivity and can be applied to polarization-independent characteristic application and gas sensors.

The invention relates to an antistatic ABS (Acrylonitrile Butadiene Styrene) plate and a preparation method thereof, and belongs to the technical field of polymer composite materials. The ABS plate is prepared from the following raw materials in parts by weight: 100 parts of ABS resin, 5.8 to 7.2 parts of a modified antistatic agent, 0.25 to 0.3 part of an antioxidant and 0.4 to 0.5 part of a lubricating agent, according to the modified antistatic agent, triethanolamine and epichlorohydrin are subjected to ring-opening and ring-closing reactions to prepare an epoxy matrix, N-phenyl p-phenylenediamine is subjected to self-polymerization to form an amino-terminated aniline tetramer, then the epoxy matrix and the aniline tetramer are subjected to ring opening to prepare a modified intermediate, and finally quaternization is performed through 2-iodoethanol; umbrella-shaped distribution is formed in an ABS matrix, so that a deep-surface layer synergistic static dissipation network is realized, and the surface performance is enhanced.

This invention relates to a highly selective ethylene tetramer Cr(III) metal catalyst system and its application method. The catalyst system comprises: a chromium compound; a ligand with the general structural formula (Ph2)P-X-P(Ph2); and an activator or co-catalyst, wherein the chromium compound is one of an organic salt, inorganic salt, coordination complex, or organometallic complex of trivalent chromium. The ethylene tetramer Cr(III) metal catalyst system and its application method provided by this invention improve upon existing PNP chromium-based catalysts, increasing the selectivity for 1-octene, reducing waxy products, and maintaining high activity.

The invention discloses a preparation method of a 2, 2, 4-trimethyl-1, 2-dihydroquinoline monomer, which comprises the following steps: dropwise adding acetone into a solution containing aniline, an acid catalyst and a polymerization inhibitor at a condensation reaction temperature, and distilling after dropwise adding to obtain the 2, 2, 4-trimethyl-1, 2-dihydroquinoline monomer; the condensation reaction temperature is 100-160 DEG C; and the distillation comprises the following steps: firstly distilling out low-boiling-point substances, then heating to 160-200 DEG C under a 1mm mercury column, and distilling out the 2, 2, 4-trimethyl-1, 2-dihydroquinoline monomer. According to the method, the polymerization inhibitor is added in the reaction process, so that the polymerization reaction of the 2, 2, 4-trimethyl-1, 2-dihydroquinoline monomer is effectively avoided, the generation of dimer, trimer and tetramer is reduced, and meanwhile, the yield of more than 80% and the purity of more than 95% can be obtained by optimizing the reaction conditions. The method does not need to use a strong corrosive acid catalyst, is simple in purification operation, and is suitable for industrial production.

The invention relates to a process for preparing a tetramer of the formula (I), or salts thereof, wherein R1is hydrogen or is a link to a solid support and PROT is an ester protecting group, to a crystalline polymorph of the tetramer of formula I and its preparation and to its use as intermediate in the preparation of a GLP-1R / GIPR agonist.

The invention discloses a deuterated glycoluril tetramer compound with aryl groups incorporated at two ends as shown in a formula (1), and a pharmaceutical composition containing the compound. The deuterated compound can be used for antagonizing a clinically used non-depolarized muscle relaxant, the antagonistic activity on rocuronium bromide and vecuronium bromide is remarkably superior to that of sugammadex sodium under the same dosage, and the antagonistic activity on cisatracurium besilate and rocuronium bromide is remarkably superior to that of a non-deuterated control molecule. # imgabs0 #

The invention belongs to a 3-hydroxybutyric acid utilization technology, discloses a preparation method of an (R)-3-hydroxybutyric acid oligomer, and aims to solve the technical problems that the (R)-3-hydroxybutyric acid oligomer is difficult to synthesize and the tetramer content in the polymer is low. The method comprises the following steps: adding (R)-3-hydroxybutyric acid crystals, a catalyst thionyl chloride and a solvent chloroform or dichloromethane into a reactor, starting stirring (such as 500 RPM), reacting at room temperature for 12-72 hours, and then removing thionyl chloride and the solvent to obtain the (R)-3-hydroxybutyric acid oligomer. The technical process provided by the invention is simple, and the catalyst thionyl chloride and the solvent (chloroform or dichloromethane) can be recovered in a reduced pressure distillation manner. The method is simple in technological process, convenient to operate and wide in industrial application prospect.

Disclosure herein relates to, inter alia, inventive cucurbituril compounds and novel methods of preparing thereof using a glycoluril compound having the formula (II), a glycoluril compound having the formula (II′), a glycoluril bis-ether of formula (III), a glycoluril dimer of formula (IV), a glycoluril tetramer compound of formula (V), and Sodium 4,4′-(1,4-phenylenebis(oxy))bis(butane-1-sulfonate, which result in significantly improved purity and yield of the intermediates and of the final products.

The invention discloses a high performance liquid detection method for glycoluril tetramer. The invention provides a high performance liquid chromatography detection method for glycoluril tetramer, which comprises the following steps: carrying out gradient elution on a glycoluril tetramer sample solution in a chromatographic column by adopting high performance liquid chromatography; the filler of the chromatographic column is octadecyl silane bonded silica gel; the mobile phase A is prepared from the following components in percentage by volume: 5 percent to 50 percent of hexafluoroisopropanol and 50 percent to 95 percent of water; the mobile phase B is prepared from the following components in percentage by volume: 20 percent to 100 percent of hexafluoroisopropanol and 0 percent to 80 percent of water. The detection method can sensitively, accurately and quickly detect the related substances and purity of the glycoluril tetramer with good reproducibility, and has outstanding technical advantages.

The present invention relates to an antistatic ABS sheet and a preparation method thereof, belonging to the technical field of polymer composite materials. The ABS sheet comprises the following raw materials, measured by weight: 100 parts of ABS resin, 5.8-7.2 parts of a modified antistatic agent, 0.25-0.3 parts of an antioxidant, and 0.4-0.5 parts of a lubricant. The modified antistatic agent is formed by ring-opening and ring-closing reactions of triethanolamine and epichlorohydrin to form an epoxy matrix, which is then self-polymerized by N-phenyl-p-phenylenediamine to form an amino-terminated aniline tetramer. The epoxy matrix and the aniline tetramer are then ring-opened to form a modified intermediate, which is finally quaternized with 2-iodoethanol. The modified intermediate forms an "umbrella-shaped" distribution in the ABS matrix, achieving a deep-surface synergistic electrostatic dissipation network and enhancing surface properties.

The present invention discloses a tetramer of Met-Pro-Ser, namely [Met-Pro-Ser]<subgt;4< / subgt;, discloses its preparation method and the therapeutic effect on testes damaged by hydrocortisone. Experiments prove that the [Met-Pro-Ser]<subgt;4< / subgt; of the present invention can not only effectively treat testes damaged by hydrocortisone, but also the curative effect of treating testes damaged by hydrocortisone is significantly stronger than that of Met-Pro-Ser in treating testes damaged by hydrocortisone. Therefore, it is proposed that the present invention provides an effective technical means for treating testes damaged by hydrocortisone.

The invention relates to a tetramer of the formula (I), or salts thereof, wherein R1 is hydrogen or is a link to a solid support and PROT is an ester protecting group or hydrogen, to a process for the preparation of a tetramer of the formula I and to its use as intermediate in the preparation of a GLP-1R / GIPR agonist.

The present invention discloses a tetramer of arbutinyl-Arg-Gly-Asp-Ser, and the tetramer has the structure of the following formula. The structure of the following formula means that the tetramer is composed of four arbutinyl-Arg-Gly-Asp-Ser molecules through non-covalent bonds. In addition, the present invention also discloses a preparation method of the tetramer of arbutinyl-Arg-Gly-Asp-Ser, as well as the anti-osteoporosis activity of the tetramer of arbutinyl-Arg-Gly-Asp-Ser and its application in the preparation of anti-osteoporosis drugs.

Disclosed are deuterated glycoluril tetramer compounds having aryl groups incorporated at both ends represented by formula (1), pharmaceutical compositions containing the compounds. The deuterated compounds are useful for antagonizing clinically used non-depolarizing muscle relaxants, and have significantly superior antagonistic activity against rocuronium and vecuronium to sugammadex at the same dose, and significantly superior antagonistic activity against cisatracurium and rocuronium to a non-deuterated control molecule.

The present invention discloses a BODIPY compound, a preparation method thereof and an application thereof. The structural formula of the compound is shown in formula (I), wherein R1 is hydrogen, halogen, heterocyclic hydrocarbon group, aryl group with 6 to 8 carbon atoms or aliphatic hydrocarbon group with 1 to 10 carbon atoms; R2 is hydrogen, aryl group with 6 to 10 carbon atoms or aliphatic hydrocarbon group with 1 to 6 carbon atoms. In the present invention, a BODIPY dimer is connected through a sulfur bridge bond to obtain a BODIPY tetramer with the structure shown in formula (I), which has a very excellent ability to generate reactive oxygen species, and can effectively inhibit the proliferation of glioma cells under light conditions, and can be used as a potential phototherapeutic drug for treating glioma. #imgabs0#

An embodiment of the present specification provides an iron isomaltose anhydride complex comprising iron oxyhydroxide and isomaltose anhydride stably associated with the iron oxyhydroxide, the content of pentamer saccharides in the isomaltose anhydride being about 16% to about 25% based on the total weight of the isomaltose anhydride, the hexamer saccharide content is from about 14% to about 23%, and the tetramer saccharide content is from about 9% to about 17%.