Present invention relates to an organic light-emitting film electrochemical deposition preparation method and application in preparing electroluminescence device, belonging to organic luminescence field. Said Light-emitting film is formed by electrochemical deposition in electrolytic bath, the monomer for electrochemical polymerization formed by chemical bond connecting electricity activity unit and luminescence unit, wherein electricity activity unit consisting of carbazole, thiophene, pyrrole, ethene, ethyne or phenylamine, diphenylamine, and triphenylamine, luminescence unit being cinnamic dimer, tripolymer, tetramer or terphenyl, quaterphenyl, quinquephenyl, tetracene, pentacene, fluorene dimer, tripolymer or tetramer and terpyridyl (dipyridyl ) rhenium etc, prepared electroluminescence device having simple technology, high device luminous efficiency, easy control area adjustable luminous color etc advantages.