The invention relates to the technical field of food fresh keeping, and particularly discloses a composition with an antibacterial function, a preparation method and application in fresh keeping of hot fresh meat, the preparation method comprises the following steps: (S01) taking
lactobacillus plantarum,
lactobacillus reuteri and fructo-
oligosaccharide, mixing, stirring, setting constant temperature, and adjusting
humidity to obtain an antibacterial matrix; (S02) mixing
sodium alginate with the bacteriostatic substrate, stirring, and adjusting the pH value to obtain a bacteriostatic structure substrate; (S03) adding a
grape seed extract, adjusting the stirring speed and duration, measuring the concentration data of each component, and testing the
solubility to obtain a functional antibacterial component; and (S04) adopting
principal component analysis, gradually adjusting the proportion of each component, judging the influence of different proportions on
bacterial colony inhibition by monitoring and analyzing the number of bacterial colonies and the inhibition rate, and obtaining an optimal proportion combination according to experimental data to obtain a preparation scheme of the composition with the antibacterial function. The invention has the characteristics of being beneficial to maintaining the health and balance of intestinal
microecology, good in safety and better in stability.