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Composition for preventing or treating inflammatory diseases, containing, as active ingredient, stem cells overexpressing sod3

A technology of inflammatory diseases and active ingredients, which is applied in the field of pharmaceutical compositions for the prevention or treatment of inflammatory diseases, and can solve problems that have not been developed

Active Publication Date: 2018-08-21
KANGSTEM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, only the general anti-inflammatory effects of MSCs have been disclosed so far, and optimized MSC stem cell therapeutic formulations that have stronger effects on inflammation-related diseases have not yet been developed.

Method used

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  • Composition for preventing or treating inflammatory diseases, containing, as active ingredient, stem cells overexpressing sod3
  • Composition for preventing or treating inflammatory diseases, containing, as active ingredient, stem cells overexpressing sod3
  • Composition for preventing or treating inflammatory diseases, containing, as active ingredient, stem cells overexpressing sod3

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0158] Preparation and efficacy identification of mesenchymal stem cells (MSC) overexpressing SOD3

[0159] Confirm the overexpression of SOD3 in MSCs transfected with SOD3

[0160] The SOD3 protein expression pattern and SOD3 enzyme activity of mesenchymal stem cells (MSC) transformed with human SOD3 gene were detected.

[0161] The human SOD3 gene was transformed by electroporation, and the protein expression level was detected by RT-PCR and Western blotting after 24 hours. The SOD3 enzyme activity was measured by measuring the amount of superoxide free radicals present in the cell culture fluid after 24 hours of culture.

[0162] Such as figure 1 As shown, compared with untransformed MSC (MSC), MSC transformed with SOD3 (SOD3-MSC) showed overexpression of SOD3 mRNA ( figure 1 A) and SOD3 protein ( figure 1 B). In addition, for SOD3 activity, SOD3-MSC showed significantly higher SOD3 activity compared with MSC ( figure 1 C). This confirms that MSCs transfected with SOD3 overexp...

Embodiment 2

[0191] Biological model of chronic rapid skin inflammation induced by imiquimod

[0192] Inhibition of MSC transformed with SOD3 on skin inflammation

[0193] based on The regulatory effects of SOD3 overexpressing MSCs on T cell differentiation and proliferation were observed in, and it was tested whether MSCs transformed with SOD3 are effective in regulating inflammation in the body.

[0194] Imiquimod (IMQ) is known to induce psoriasis and similar acute skin inflammation through signals that activate the innate immune system. It is applied to the back skin of depilated mice daily to induce skin Inflammation. according to Picture 10 The summary of the experiment, before smearing IMQ, according to the processing conditions, the untreated MSC, LacZ-transduced MSC and SOD3-transduced MSC each press 2×10 6 After subcutaneous injection into mice in each experimental group (5 mice in each group), and continuous administration of IMQ for 12 days, the inflammatory response on the skin...

Embodiment 3

[0214] Biological model of atopic dermatitis induced by ovalbumin

[0215] By using other animal models, the effect of SOD3 overexpressing MSC on inflammation in vivo was confirmed. Ovalbumin (OVA) was used as an antigen to induce atopic dermatitis in Balb / C mice, and the inhibitory effect of MSC on inflammation was observed.

[0216] according to Picture 20 The summary of the experiment. At the beginning of the experiment (D0), day 7 (D7), and day 14 (D14), OVA protein and the antigen adjuvant aluminum hydroxide were mixed and injected into the abdominal cavity of mice raised under SPF conditions. Sensitize mice. From the 21st day of the experiment, dissolve 100μg of OVA in 100μl of PBS and immerse 1×1cm 2 The gauze preparation patch was attached to the back of depilated mice to induce an immune response for 7 days. Starting from day 35, the OVA patch was used to induce an immune response in the same way again for a week. On the 42nd day after the start of the experiment, M...

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PUM

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Abstract

The present invention provides a pharmaceutical composition for preventing or treating inflammatory diseases, containing, as an active ingredient, stem cells overexpressing SOD3. The present inventorhas ascertained that mesenchymal stem cells (MSCs) overexpressing SOD3 have a stronger antioxidant activity and immune regulatory function than normal MSCs, and thus the MSCs overexpressing SOD3 can be an effective treatment agent for inflammatory diseases, autoimmune diseases, organ transplant rejection and the like.

Description

Technical field [0001] This application claims the priority of Korean Patent Application No. 10-2015-0130377 filed on September 15, 2015. At the same time, the entire content of the description is the reference of the application. [0002] The present application relates to a composition for the prevention or treatment of an inflammatory disease comprising stem cells overexpressing SOD3 as an active ingredient, and more specifically, to an inflammatory disease comprising stem cells overexpressing SOD3 (extracellular superoxide dismutase) as an active ingredient A pharmaceutical composition for the prevention or treatment of diseases. Background technique [0003] Inflammatory diseases are caused by various external stimuli or internal factors that cause inflammatory immune cell infiltration, swelling, redness, and pain in various organs or tissues of the human body, and show histological changes. These inflammations are caused by a variety of chemical mediators produced by damaged...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K35/50A61K35/51A61P37/02A61P37/06A61P27/02A61P1/00A61P17/00A61P37/08C12N15/85C12N5/0775
CPCA61K35/50A61K35/51C12N15/85A61K35/28A61P17/00A61K38/446C12Y115/01001Y02A50/30C12N5/0662
Inventor 康景宣金泰润
Owner KANGSTEM BIOTECH
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