77 results about "Ovalbumin" patented technology

The present invention belongs to the technical field of cancer immunotherapy, and particularly relates to a cholesterol-modified cationic liposome tumor vaccine, a preparation method therefor, and use thereof. In order to solve the problems of poor targeting and strong side effects of TLR agonists in anti-tumor treatment, the present invention provides a cationic liposome prepared from a cholesterol-modified 1V209 molecule, a cationic lipid component, cholesterol, and DSPE-PEG2000, and then the cationic liposome and ovalbumin 5 form the tumor vaccine by electrostatic adsorption. Animal experiments show that the vaccine can induce antigen-specific CD8+ T cells, activate lymphocytes, and generate stronger antigen cross-presentation, more memory T cells, antibodies, and cytokines. Prophylactic inoculation with the vaccine can significantly delay the progression of mouse melanoma and lymphoma and prolong the survival of mice. The combination use of the vaccine and a PD-1 checkpoint inhibitor can further enhance the anti-tumor effect. Therefore, the vaccine is a promising cancer vaccine.

The application belongs to the field of nanometer material in environmental monitoring application, and discloses a fluorescence immunoassay method for detecting ovalbumin concentration based on carbon quantum dots, which comprises the following steps: using L-glutamic acid as a precursor, one-step preparation of carbon quantum dots with amino groups by ultrasonic hydrothermal method; using a reduced graphene oxide method to prepare graphene sheets, and optimizing the graphene by modifying polydiallyldimethylammonium chloride to obtain graphene sheets which can have good fluorescence resonance energy transfer with the carbon quantum dots with amino groups; and coupling the prepared carbon quantum dots with amino groups with a monoclonal antibody. The application directly prepares carbon quantum dots with amino groups by one-step hydrothermal method, and the quantum dots can be directly coupled with a monoclonal antibody. The carbon quantum dots involved in the application avoid the aminoization step, and the linear range can reach 0.1 ng / mL-3000 ng / mL.

The invention provides a pH response type composite hydrogel dressing as well as a preparation method and application thereof, and belongs to the technical field of medical materials. According to the pH response type composite hydrogel dressing, gel formed by crosslinking glycyrrhetinic acid and ovalbumin amyloid fibrils is used as a carrier, and a zinc ion modified metal organic framework material is loaded on the carrier. Functional groups such as carboxyl and hydroxyl of glycyrrhetinic acid are combined with ovalbumin amyloid fibrils through hydrogen bonds and electrostatic interaction to form a hydrogel carrier, and then a zinc ion modified metal organic framework material is loaded on the hydrogel carrier to obtain the composite hydrogel dressing. The hydrogel has antibacterial and anti-biofilm effects on escherichia coli and staphylococcus aureus, has good biocompatibility and mechanical properties, has pH responsiveness, and can realize programmed control to actively remove bacteria.

The invention relates to the technical field of packaging materials, in particular to an intelligent packaging film based on a multilayer packaging nanocomposite and a preparation method of the intelligent packaging film. On the basis of the anthocyanin CD-MOFs (core) with a physical adsorption function, an ovalbumin-sodium alginate (OVA-SA) nano-composite is innovatively introduced to construct a core-shell structure, and an OVA-SA shell and a CD-MOFs pore channel form a double barrier, so that the stability of the anthocyanin is remarkably improved; the OVA-SA shell is used as an interfacial compatibilizer, and the high chemical affinity of the OVA-SA shell and a corn starch / PVA matrix is utilized, so that nano-scale uniform dispersion of MOF particles in the matrix is realized, and agglomeration is effectively avoided; meanwhile, an active pH response gated release mechanism is formed, high stability is kept in the shelf life, shell response is triggered only when a decay product induces pH rise, anthocyanin is released, color development is carried out, and sensitive monitoring on the freshness of the high-protein food is achieved.

The present application relates to the field of biological medicine, in particular to a sensitizing agent for constructing a neutrophilic asthma animal model, a construction method and application. The present application uses Haemophilus influenzae outer membrane vesicles and ovalbumin as a sensitizing agent to construct a neutrophilic asthma animal model, and the test results show that the level of neutrophils in the blood and lung lavage fluid of mice is significantly increased compared with the treatment of ovalbumin as a sensitizing agent alone. The constructed mouse neutrophilic asthma model can be used for the research on the mechanism of clinical neutrophilic asthma and the development of neutrophilic asthma drugs.

The invention belongs to the technical field of food processing, and relates to a preparation method and application of astaxanthin emulsion gel. The invention provides a preparation method of astaxanthin emulsion gel, which comprises the following steps: taking an ovalbumin and beet pectin compound solution obtained through electrostatic interaction under a specific pH value as a water phase, taking medium-chain fatty acid containing astaxanthin as an oil phase, and mixing the oil phase with the ovalbumin and beet pectin compound solution to obtain the astaxanthin emulsion gel. The preparation method comprises the following steps: carrying out homogenization treatment on a blend of a water phase and an oil phase by using a high-speed dispersion machine to obtain a primary emulsion, carrying out ultrasonic treatment on the primary emulsion to obtain an emulsion, adding calcium ions into the emulsion, uniformly mixing, and carrying out crosslinking and phase change on the emulsion to obtain the astaxanthin emulsion gel. According to the astaxanthin emulsion gel disclosed by the invention, the storage stability of the astaxanthin can be remarkably improved, so that more astaxanthin is migrated to a water phase in an intestinal digestion process, the bioavailability of the astaxanthin is improved, and the antioxidant activity in vivo is remarkably improved compared with that of free astaxanthin.

This invention provides a reagent kit, its preparation method, and its application, as well as a method for detecting acetochlor, relating to the fields of analytical chemistry and biosensor technology. The reagent kit includes a detection solution and a test strip; the detection solution includes CuCo@PDA conjugated with an anti-acetochlor antibody; the test strip includes a sample pad, a detection pad, and an absorbent pad arranged sequentially; from the sample pad to the absorbent pad, the detection pad has a detection line and a control line arranged sequentially; the detection line is immobilized with an ATC-BSA conjugated antigen and protein-modified ATT-AuNCs; the protein includes BSA or ovalbumin; the control line is immobilized with a secondary antibody that specifically recognizes the anti-acetochlor antibody. This reagent kit utilizes the internal filtration effect to construct a dual-mode detection mechanism of "off" colorimetric signal / "on" fluorescence signal, and the signal can be collected and analyzed via a smartphone, making it suitable for rapid on-site screening of acetochlor residues in traditional Chinese medicinal materials.

The invention discloses a construction method of an animal model for combined induction and exacerbation of asthma. The method comprises the following steps: injecting ovalbumin or dermatophagoides pteronyssinus extract into an intraperitoneal cavity for sensitization; aerosol inhalation of allergen is carried out for airway re-exposure; in the aggravating stage, pathogenic microorganisms such as pseudomonas aeruginosa, aspergillus fumigatus or respiratory syncytial virus are given and exposed to 500 mu g / m PM2.5 aerosol; lung tissue and bronchoalveolar lavage fluid are then detected. The established model shows that the airway resistance is obviously enhanced and persistent, the mucous thrombus formation rate is greater than 50%, the smooth muscle is obviously thickened, and the model is accompanied by eosinophilic granulocyte and neutrophil infiltration and high expression of various inflammatory factors. The model is good in stability and high in repeatability, can truly simulate clinical asthma acute exacerbation and steroid insensitive characteristics, and provides a platform for asthma mechanism research and new drug screening.

The invention discloses a small-molecule active aldehyde scavenger-albumin coupling medicine as well as a preparation method and application of the small-molecule active aldehyde scavenger-albumin coupling medicine. The coupling drug comprises a) the small molecule active aldehyde scavenger compound or the derivative thereof and b) albumin, and the albumin comprises at least one of human serum albumin, recombinant albumin, bovine serum albumin, ovalbumin and mouse serum albumin. The medicine is further prepared into an ophthalmic preparation, the retention time of the medicine in the cornea is prolonged, the in-vitro active aldehyde removal efficiency of the medicine is improved to 1.5 times of that of NS-2, expression (larger than or equal to 50%) of a human corneal epithelial cell inflammatory factor IL-6 is remarkably inhibited, and irritation to eyes is avoided. Through the synergistic effect of long-acting slow release of the albumin carrier and targeted removal of NS, the technical defects that a traditional xerophthalmia medicine is short in effect and single in mechanism are overcome, and a definite clinical transformation value is achieved.

The invention discloses a preparation method of a double-protein aggregate for packaging and stabilizing spice essential oil, the double-protein aggregate and application of the double-protein aggregate. According to the process, ovalbumin and lysozyme are used as raw materials, stock solutions of the ovalbumin and the lysozyme are mixed in proportion, then pH adjustment and NaCl concentration adjustment are conducted, pulsed electric field treatment is introduced, protein is promoted to be subjected to liquid-liquid phase separation under the synergistic effect to form a composite condensate, and the composite condensate is used for packaging and stabilizing spice essential oil. Through multi-dimensional synergistic regulation, the performance of the composite coagulator is remarkably improved, the embedding rate of the emulsion to the spice essential oil reaches 90% or above, the particle size increase is smaller than or equal to 10% after the emulsion is refrigerated for 6 months at 4 DEG C, the layering rate is smaller than or equal to 5%, the release rate of irritant peculiar smell components in the spice essential oil can be effectively reduced, the sensory characteristics of the spice essential oil can be greatly improved, and the flavor of the spice essential oil can be improved. The application range in the fields of food, health care products and the like is expanded.

The invention discloses an OVA-D fluorescence immunochromatography detection test strip in a surimi product and application thereof, the test strip comprises a substrate, a sample adding pad, a combination pad, a reaction film and a water absorption pad, the sample adding pad, the combination pad, the reaction film and the water absorption pad are arranged on the substrate, the reaction film is arranged in the middle of the substrate, one end of the sample adding pad is in contact with the other end of the combination pad, one end of the combination pad is in contact with the other end of the reaction film, and the other end of the reaction film is in contact with the water absorption pad. One end of the reaction film contacts with the other end of the water absorption pad; the combination pad contains a rabbit anti-OVA-D polyclonal antibody marked by fluorescent quantum dots and goat IgG marked by fluorescent quantum dots; the reaction film is provided with a detection area, the detection area is sequentially provided with a detection line and a control line from the other end to one end of the reaction film, the detection line is coated with an OVA-D antigen, and the control line is coated with a rabbit anti-goat IgG antibody. The fluorescence signal is stable, the fluorescence intensity of the T line and the C line is not influenced by a complex matrix in a detected sample, the detection sensitivity is higher, and the reliability of quantitative detection of the duck egg ovalbumin is also ensured.

The application belongs to the technical field of laser, and relates to a method for manufacturing a PVA / dye flexible random laser based on an electrostatic spinning method. A mixed solution of PVA, ovalbumin, rhodamine dye and Au nano-particles is adopted, the sample size and the morphology of the spinning structure are controlled by controlling the voltage, the spraying distance and the needle tip size in the electrostatic spinning technology process, and then the wavelength and the mode of laser emission and other parameters are adjusted. The design is simple and intuitive, the method is flexible and convenient, the principle is reliable, and the method is easy to realize. The obtained flexible random laser is easy to be coupled with other photon devices, and the quantitative control of the laser property is easy to be realized.

The invention relates to a biocompatible skin wound healing medicine and a preparation method thereof, and belongs to the technical field of biological medicine, and the medicine comprises liposome-clathrated ovalbumin peptide, a red abelmoschus esculentus seed extract, a taro stem extract, liposome-clathrated acetyl tetrapeptide-9 and vitamin E. The ovalbumin peptide is prepared by the following steps: pre-cooling ovalbumin of goose eggs, sequentially carrying out enzymolysis on the pre-cooled ovalbumin through trypsin and ginger protease, and purifying the enzymatic hydrolysate through an XAD16 nonionic macroporous resin column. The hibiscus coccineus seed extract is obtained by performing ultrasonic enzymolysis on hibiscus coccineus seeds in an ethanol water solution through hemicellulase and ginger protease and purifying enzymatic hydrolysate through an AB-8 macroporous adsorption resin column. The taro stalk extract is obtained by grinding taro stalks into pulp, performing ultrasonic extraction with an ethanol aqueous solution, and purifying an extracting solution with an HP-20 macroporous adsorption resin column. All the components are synergistically used, a multifunctional integrated repair system is constructed, and the problems that a traditional medicine is insufficient in biocompatibility, single in function and low in repair efficiency are solved.

The invention belongs to the technical field of protein peptide powder. The invention provides a preparation method of ovalbumin peptide powder, which comprises the following steps: S1, transferring protein liquid into a fermentation tank, adjusting the pH value to 6.0-6.9, adding dry yeast, stirring and fermenting to obtain fermentation liquid; s2, transferring the fermentation liquor obtained in the S1 into an enzymolysis tank, heating to 50-55 DEG C, adding a compound enzyme preparation, carrying out enzymolysis for 7-10 hours, and stirring for 10 minutes per hour to obtain enzymatic hydrolysate; the compound enzyme preparation comprises papain, subtilisin, trypsin and aminopeptidase; and S3, transferring the enzymatic hydrolysate obtained in S2 back to a fermentation tank, cooling, adding dry yeast, stirring, fermenting, spray-drying, and placing in a hot chamber for 3-7 days to obtain the product. According to the ovalbumin peptide powder, the oxidation resistance and the heat resistance are improved at the same time, so that the ovalbumin peptide powder with more excellent functionality and wider application range is obtained.

The invention discloses a method for synchronously separating ovalbumin, ovotransferrin and lysozyme from egg white, which comprises the following steps: firstly, adsorbing ovalbumin from egg white loading liquid by taking chitosan-polybutyl methacrylate nanocrystal gel with an anion exchange function as a primary separation medium, and carrying out gradient elution to obtain ovalbumin; taking a primary separation loading effluent as a secondary separation loading solution; hydroxyethyl methylacrylate-polyglycidyl methacrylate nanocrystal gel with a cation exchange function is taken as a secondary separation medium to adsorb ovotransferrin and lysozyme from a secondary separation loading solution, gradient elution is performed by gradually increasing salt concentration, and separation of ovotransferrin and lysozyme is realized. The method provided by the invention can realize synchronous separation of ovalbumin, ovotransferrin and lysozyme, has the advantages of high separation efficiency, simplicity and convenience in operation, low energy consumption and the like, and is suitable for industrial large-scale production, and the separation medium can be repeatedly used.

The invention belongs to the technical field of food microorganisms, and particularly relates to a lactobacillus paracasei BHP06 with an anti-allergic function. The preservation number of the Lactobacillus paracasei BHP06 is GDMCC (China General Microbiological Culture Collection Center) No: 66270, and the preservation number of the Lactobacillus paracasei BHP06 is CGMCC No: 66270. The lactobacillus paracasei strain provided by the invention has good gastrointestinal tract reverse environment resistance and has good survival ability in simulated gastrointestinal fluid; good self-aggregation capability and hyaluronidase inhibition capability are realized. By establishing a degranulation model of RBL-2H3 cells, it is found that the strain can inhibit mast cell degranulation and relieve allergy; by establishing an RAW264.7 cell inflammation model, the strain disclosed by the invention is found to be capable of inhibiting allergic inflammatory reaction; by establishing an OVA (ovalbumin)-induced BALB / c mouse food allergy model, the strain disclosed by the invention is found to be capable of improving the anti-allergy ability of animals and exerting the activity of relieving food allergy.

The invention belongs to the technical field of nano antibodies, and provides a nano antibody OVA-3-23 for resisting chicken ovalbumin and application of the nano antibody OVA-3-23. The nano antibody OVA-3-23 for resisting the chicken ovalbumin has an amino acid sequence as shown in SEQ ID NO. 1. According to the prepared nano antibody OVA-3-23 for resisting the chicken ovalbumin, in the field of directional fixation, accurate anchoring of the chicken ovalbumin is achieved with high specificity, and the defects of impurity interference and fuzzy positioning in traditional fixation are overcome; in the field of immunodetection, high specificity and strong binding force guarantee the detection accuracy, colloidal gold, enzyme and other multi-component components are matched, and the chicken ovalbumin is efficiently identified from food on-site fast screening to laboratory fine detection; in the field of enrichment and purification, a high-purity product is efficiently separated from a complex sample. Especially, reliable support is provided for application scenes such as food detection, feed quality monitoring, immunology and vaccine development, and an efficient solution is provided for detection, research and quality control related to chicken ovalbumin.

The invention discloses photo-thermal controlled-release injectable hydrogel for promoting wound healing through nerve and blood vessel synergistic regeneration as well as a preparation method and application of the photo-thermal controlled-release injectable hydrogel. The preparation method comprises the following steps: preparing an ovalbumin electrostatic spinning fiber membrane; the preparation method comprises the following steps: modifying an ovalbumin electrostatic spinning fiber membrane with dopamine, grafting deferoxamine, and shearing and grinding to obtain the drug-loaded short fiber capable of providing a topological structure to guide cell growth; the preparation method comprises the following steps: mixing drug-loaded short fibers with a multi-walled carbon nanotube dispersion liquid, preparing micro-droplets through a microfluidic device, and carrying out cross-linking curing to obtain photothermal responsive drug-loaded short fiber microspheres; the preparation method comprises the following steps: blending an ovalbumin hydrogel precursor solution with photo-thermal responsive drug-loaded short fiber microspheres, and carrying out catalytic molding through a cross-linking agent under a heating condition. According to the invention, the photo-thermal response microspheres are combined with the hydrogel, so that the regeneration microenvironment is synergistically regulated and controlled by multiple factors, and a new thought is provided for tissue engineering nerve grafts. The method has the characteristics of no toxicity, harmlessness, environmental protection, simplicity, feasibility and easiness in operation.

The invention relates to the field of biotechnology and food science, in particular to an ovalbumin sensitization active epitope analysis method. According to the invention, an integrated analysis process from enzymolysis, separation and activity evaluation to sequence identification is constructed. According to the method, enzymolysis products are closely combined with sensitization activity evaluation of a cellular / immunological level according to molecular weight system separation for the first time, target peptide fragments with high sensitization activity can be actively'screened 'instead of'predicted' from a complex enzymolysis mixture, and finally, the amino acid sequences of the target peptide fragments are accurately identified through technologies such as mass spectrum and the like. Compared with a prediction method depending on computer simulation, the method is completely based on experimental evidence, the obtained sensitization activity epitope sequence is real and reliable, the sensitization core area still reserved after actual enzymolysis treatment of ovalbumin is directly reflected, and a solid experimental data support is provided for understanding the sensitization mechanism of ovalbumin.

The invention relates to the technical field of antianaphylaxis, in particular to an antianaphylaxis polymer and application of the antianaphylaxis polymer as a skin repairing agent.The antianaphylaxis polymer is composed of chitosan, a tannic acid-ovalbumin compound and amination modified graphene quantum dots; the particle size of the amination modified graphene quantum dots is 5-20 nm, the surface amino density is 3.5-5.0 mmol / g, and the addition amount of the amination modified graphene quantum dots is 0.5-1% of the mass of ovalbumin protein; the mass ratio of the chitosan to the tannic acid-ovalbumin compound is 1: (0.5-0.8); the anti-allergic polymer prepared by the scheme has excellent anti-allergic performance and skin repair effect, all components of the cream prepared by compounding the anti-allergic polymer with specific auxiliary materials are selected from natural materials or materials with good biocompatibility, the preparation process is mild and controllable, no harmful by-product is generated, and the product is mild and non-irritant in use and is suitable for long-term use.

The invention discloses a porous starch-self-assembled polyphenol nano-carrier and a preparation method thereof, and the preparation method comprises the following steps: (1) encapsulating polyphenol in ovalbumin-sodium carboxymethyl cellulose nano-particles through an anti-solvent method to obtain polyphenol-loaded OVA / CMC nano-particles; and (2) integrally encapsulating the polyphenol-loaded OVA / CMC nanoparticles in micropores of porous starch to obtain the polyphenols-loaded OVA / CMC nano-porous starch. Porous starch adsorption and nano-particle loading are combined, firstly, polyphenol is encapsulated in OVA / CMC nano-particles through an anti-solvent method, then the nano-particles loaded with the polyphenol are used as a whole to be encapsulated in micropores of porous starch, and a novel porous starch-self-assembled nano-particle polyphenol loading system is formed.

The invention discloses a water-in-water emulsion delivery system based on interface bionic reinforcement and multistage protection and application thereof, and belongs to the technical field of functional factor delivery. According to the system, an amylopectin aqueous solution is used as a continuous outer phase, a caesalpinia spinosa gum aqueous solution is used as a dispersed inner phase, and a full-water-phase emulsion system with a double-layer interface structure is constructed through layer-by-layer electrostatic assembly of fibrous ovalbumin particles and carrageenan. The emulsion can efficiently entrap hydrophilic functional factors such as probiotics, vitamins, active peptides and the like, and the light stability, the heat stability, the ion tolerance and the stability in the processing and storage process of the emulsion are remarkably improved. The emulsion can be further freeze-dried into microcapsule powder, is suitable for functional beverages, dairy products, solid granules, nutritional supplements and other foods, and can also be used in essence, facial masks and other cosmetic dosage forms. The system has the characteristics of controllable structure, high stability, safe raw materials, green process and the like, and is suitable for the development of high-stability and high-bioavailability products.

This invention relates to the field of pet product technology, specifically to a cat-specific nutritional pet food for shiny, strong, and healthy fur and its preparation method. This invention, through the compounding of vitamins, amino acids, oils, proteins, microorganisms, plant-derived nutrients, and a fur-strengthening additive, yields a nutritionally balanced food that promotes shiny, strong, and healthy fur in pets. The fur-strengthening additive is obtained by mixing fur-strengthening additive A and fur-strengthening additive B. Fur-strengthening additive A directly provides the substances needed for hair growth, improves the pet's hydration, promotes healthy fur, and effectively enhances the pet's immunity and disease resistance. In fur-strengthening additive B, coenzyme Q10-ovalbumin amyloid fiber complex works synergistically with vitamin 6 and taurine to effectively reduce free radical damage, delay cell aging, and thus improve hair quality and health, showing broad application prospects.

This disclosure provides expression systems comprising secretion signals that promote production and / or secretion of proteins of interest, as well as one or more polynucleotides encoding chaperone proteins (e.g., CRT and / or PDIA3) which, as demonstrate herein, enhance production and / or secretion of proteins. Moreover, genetically modified host cells comprising these expression systems are capable of producing high levels of protein of interest, such as bovine lactoferrin (bLF), bovine lactoglobulin (bLG), or ovalbumin (Ova).

The invention discloses an edible and medicinal composition solid beverage and a preparation method thereof. The beverage is prepared from the following raw materials in percentage by weight: 45-60% of a traditional Chinese medicine extract, 10-18% of energy and flavor components, 27-38% of a sweetening agent and 0.01-0.5% of a stabilizer. The energy and flavor components of the edible and medicinal composition solid beverage disclosed by the invention adopt coconut oil powder and green kumquat raw powder, so that the taste is suitable for most people; the sweetening agent is a combination of erythritol, stevioside and sweet essence, so that sugar metabolism in the body is avoided, and the worry of rising sugar is avoided; starch sodium octenylsuccinate, ovalbumin, locust bean gum and abelmoschus esculentus colloid polysaccharide are adopted as stabilizers, so that the beverage is uniform and stable after being flushed with water and is not easy to settle.

A cell growth promoter and an application thereof are provided. The invention adopts Fe2+ and ovalbumin to form a Fe2+-ovalbumin (OVA) compound to promote ovalbumin cell endocytosis, the cell growth promoter can maintain cell growth in an environment free of amino acids, promote cell proliferation in an environment with amino acids; and, the Fe2+-OVA compound can replace expensive animal serum albumin in cell culture, the cell growth promoter has a significant effect on improving cell viability and is low cost.

The invention discloses a method for detecting ovalbumin through an electrochemical bionic small intestine micro-fluidic chip sensor and application, and belongs to the technical field of food safety detection. A micro-intestinal chip is designed by simulating the structure of the small intestine of a human body, an anti-IL-6 antibody is integrated on a screen-printed electrode to serve as a specific recognition element, a sensor capable of being used for specifically recognizing cell secretion IL-6 is constructed, and effective and indirect measurement of OVA is achieved. An OVA standard substance is used for testing the bionic small intestine sensor, a good linear relation exists between the resistance value detected by an electrochemical means and the OVA concentration, the linear detection range is 0.2 pg / mL to 4 pg / mL, the detection limit is 0.161 pg / mL, and it is proved that the bionic small intestine sensing interface is feasible and the sensor can conduct quantitative sensitive detection on the allergen protein.

The invention provides a combined vaccine based on Th1 / Th2 immune dynamic equilibrium as well as a preparation method and application of the combined vaccine. Subcutaneous delivery of interleukin 12 (IL12) and antigen (such as ovalbumin OVA) mRNA is adopted in the early stage of tumor occurrence, so that specific antigen recognition and T cell immune response of an organism are activated; when immune response enters a function decline stage, the tumor microenvironment is regulated through intratumor delivery of interleukin 4 (IL4) mRNA, the metabolic activity of T cells is maintained, and the depletion of the T cells is delayed. Through space (subcutaneous / intra-tumor) and time (early stage / middle stage) separated delivery of different immune factors, 'start-steady state maintenance 'type immune stylization regulation and control are realized, antigen-specific CTL reaction and persistent anti-tumor effect are remarkably enhanced, and the problem of immune bias caused by independent use of the antigen-specific CTL reaction and the persistent anti-tumor effect is solved.

The invention discloses an antibody drug lung in-vivo and in-vitro tracing detection method based on gold nanocluster fluorescence and CT bimodal imaging performance. A prepared Rayleizumab-gold nanocluster presents red fluorescence, the maximum emission wavelength is 653 nm, the quantum yield reaches 14.4%, and the Rayleizumab-gold nanocluster has excellent photobleaching resistance. Meanwhile, the Rayleizumab-gold nanocluster also has a CT (Computed Tomography) imaging function and retains the biological activity of the antibody. In an ovalbumin-induced asthma mouse model, after non-invasive administration of the Rayleizumab-gold nanocluster into the trachea of a mouse, it can be observed that the CT signal of the lung of the mouse is enhanced and the CT density value of the lung tissue of the mouse in an asthma group is higher than that of a normal group and is consistent with the change trend of a fluorescence imaging result. In conclusion, by fusing the high sensitivity of fluorescence imaging and the high spatial resolution of CT imaging, accurate visual evaluation of the lung delivery efficiency of the antibody drug and the metabolic kinetics is achieved, and technical reference is provided for research, development and clinical transformation of the antibody inhalant.