133 results about "Ovalbumin" patented technology

The present invention relates to a method for producing a canine atopic dermatitis animal model, a canine atopic dermatitis animal model produced thereby, and a use thereof. According to the present invention, a canine atopic dermatitis animal model is produced by skin stimulation on a dog through epicutaneous sensitization with ovalbumin at a concentration of 1 to 5 mg / ml once daily for 1 to 2 weeks and then with ovalbumin at a concentration of 1 to 5 mg / ml once daily for 2 to 8 weeks. The resulting animal model exhibits clinical symptoms of atopic dermatitis, changes in relevant factors such as increased expression levels of IgE and CCL17, and elevated expression levels of Th2-related cytokines IL-4, IL-31, and IL-13. Therefore, this invention enables the establishment of a standardized animal model for symptoms and Th2 immune responses appearing upon the onset of atopic dermatitis, and the model can be used in clinical studies for screening candidate agents for the prevention, alleviation, or treatment of atopic dermatitis.

The present invention belongs to the technical field of cancer immunotherapy, and particularly relates to a cholesterol-modified cationic liposome tumor vaccine, a preparation method therefor, and use thereof. In order to solve the problems of poor targeting and strong side effects of TLR agonists in anti-tumor treatment, the present invention provides a cationic liposome prepared from a cholesterol-modified 1V209 molecule, a cationic lipid component, cholesterol, and DSPE-PEG2000, and then the cationic liposome and ovalbumin 5 form the tumor vaccine by electrostatic adsorption. Animal experiments show that the vaccine can induce antigen-specific CD8+ T cells, activate lymphocytes, and generate stronger antigen cross-presentation, more memory T cells, antibodies, and cytokines. Prophylactic inoculation with the vaccine can significantly delay the progression of mouse melanoma and lymphoma and prolong the survival of mice. The combination use of the vaccine and a PD-1 checkpoint inhibitor can further enhance the anti-tumor effect. Therefore, the vaccine is a promising cancer vaccine.

The invention relates to the field of analysis, in particular to an ovalbumin molecular weight analysis method and application thereof. The method for analyzing the molecular weight of the ovalbumin comprises the following steps: (a) performing denaturation treatment and cooling on an ovalbumin solution, and adding glycosidase for enzyme digestion to obtain an ovalbumin desugaring sample; (b) detecting and analyzing the ovalbumin desugaring sample by using liquid chromatography and mass spectrometry; and (c) carrying out molecular weight analysis according to detection data obtained by the mass spectrum. According to the method for analyzing the molecular weight of the ovalbumin, the desugaring step is simplified, and the desugaring effect is improved; the analysis time is short, the operation is simple, and only several hours are needed from sample pretreatment to result analysis completion.

The invention belongs to the technical field of aquatic product preservation, and particularly relates to a protein-based intelligent gel label with aquatic product freshness indication and mechanical properties as well as a preparation method and application of the protein-based intelligent gel label. The preparation method comprises the following steps: mixing an ovalbumin solution with a chitosan-anthocyanin compound solution, and incubating under the action of a Ca < 2 + > ion slow release system of calcium sulfate. The intelligent gel label for indicating the freshness of the aquatic products is applied to monitoring of the freshness of the shrimps, the color of the intelligent gel label changes along with the decay degree of the shrimps, the intelligent gel label correspondingly shows different color changes under different trimethylamine concentrations, TVC values, pH values and TVB-N values, and the intelligent gel label has the advantages of being high in sensitivity, good in mechanical performance and high in stability; the food-grade safe and reliable visual monitoring of the freshness of the aquatic products is realized.

The invention discloses bifidobacterium bifidum and application of the bifidobacterium bifidum in relieving ovalbumin-induced food allergy, and belongs to the technical field of microorganisms. According to the invention, a strain of bifidobacterium bifidum CCFM1063 is screened out, and the bifidobacterium bifidum CCFM1063 has the effects of relieving weight loss and allergic symptoms of mice caused by OVA; according to the present invention, with the application of the loratadine, the high mMCP-1 level caused by OVA can be significantly reduced, the IgE level can be reduced, the mRNA level of the jejunum allergy related cytokines such as TSLP, IL-4 and IL-33 can be significantly reduced, and the partial effect is superior to the loratadine; when the bifidobacterium bifidum CCFM1063 is combined with breast milk oligosaccharide 3 '-SL, the bifidobacterium bifidum CCFM1063 has a synergistic effect on improving food allergy symptoms, and the effect of the bifidobacterium bifidum CCFM1063 is obviously superior to that of a single strain.

The invention discloses a terahertz metasurface biosensor which is modified by a single-stranded DNA (deoxyribonucleic acid) nucleic acid aptamer and is used for specifically detecting a tumor necrosis factor-alpha. Under the simulation condition, the surface of the biosensor is covered with a layer of analyte with the thickness of 20 microns, and when the refractive index of the analyte is changed from n = 1 to n = 2, the theoretical sensitivity of the metasurface biosensor can reach 124.71 GHz / RIU. In an experiment, the metasurface is prepared on a copper-plated polyethylene glycol terephthalate sheet by using photoetching and chemical corrosion processes. Then, according to the characteristic that the nucleic acid aptamer is specifically combined with the tumor necrosis factor-alpha, the nucleic acid aptamer is modified on the surface of the sensor, so that the super-surface biological immunosensor is prepared, the concentration detection of the tumor necrosis factor-alpha is realized, the detection limit reaches 0.1 ng / ml, and the detection sensitivity can reach 13.9 GHz / 1g (ng / ml). Besides, ovalbumin and whey protein with the same concentration are compared and detected, and the result shows that only the tumor necrosis factor-alpha can be obviously distinguished, so that the sensor has a specific detection function. The terahertz metasurface immunosensor provided by the invention realizes the specific detection of the tumor necrosis factor-alpha. The work can be used for biomarker detection and early diagnosis of diseases.

The invention relates to the technical field of health-care food, and discloses a health-care composition containing microbial beneficial bacteria and a preparation method of the health-care composition. Comprising the following components in parts by weight: 30-40 parts of gelatinized starch, 8-15 parts of plant enzyme powder, 2-10 parts of microcapsule compound beneficial bacteria, 3-5 parts of selenium-enriched yeast powder, 0.5-2 parts of an American ginseng extract, 12-20 parts of fructo-oligosaccharide, 1-2.5 parts of curcumin, 7-9 parts of ovalbumin and 5-8 parts of sodium carboxymethyl cellulose. Microcapsule composite beneficial bacteria are prepared by compounding bifidobacterium longum, lactobacillus acidophilus, lactobacillus rhamnosus and lactococcus lactis, bridging with carboxymethylated beta-glucan and a Fe < 3 + >-polyphenol network structure and embedding with a protein-polysaccharide system, and can stably exist in an environment with strong acid and high-concentration bile salt to gastrointestinal tracts; through the synergistic effect of different nutrient substances, the intestinal microbe balance can be improved, and the immunity can be remarkably improved.

The invention discloses a method for rapidly and efficiently extracting ovotransferrin in egg white, which comprises the following steps: (1) pre-treating egg white, namely treating egg white liquid through an ultrasonic composite high-voltage pulse electric field, so that the thick protein is thinned, the viscosity is further reduced, molecules are dispersed, the egg white liquid is prevented from being diluted by adding water in the subsequent process, and the production efficiency is favorably improved; (2) removal of ovomucin and ovalbumin: removing the ovomucin and ovalbumin by an isoelectric precipitation method combined with a PEG precipitation method; (3) preparation of ovotransferrin; (4) separating an ovotransferrin crude product; and (5) purifying the ovotransferrin crude product. According to the method disclosed by the invention, high-yield and high-purity ovotransferrin is rapidly and efficiently prepared, and the method is simple, relatively low in cost and suitable for large-scale industrialization.

The application belongs to the field of nanometer material in environmental monitoring application, and discloses a fluorescence immunoassay method for detecting ovalbumin concentration based on carbon quantum dots, which comprises the following steps: using L-glutamic acid as a precursor, one-step preparation of carbon quantum dots with amino groups by ultrasonic hydrothermal method; using a reduced graphene oxide method to prepare graphene sheets, and optimizing the graphene by modifying polydiallyldimethylammonium chloride to obtain graphene sheets which can have good fluorescence resonance energy transfer with the carbon quantum dots with amino groups; and coupling the prepared carbon quantum dots with amino groups with a monoclonal antibody. The application directly prepares carbon quantum dots with amino groups by one-step hydrothermal method, and the quantum dots can be directly coupled with a monoclonal antibody. The carbon quantum dots involved in the application avoid the aminoization step, and the linear range can reach 0.1 ng / mL-3000 ng / mL.

The invention provides a pH response type composite hydrogel dressing as well as a preparation method and application thereof, and belongs to the technical field of medical materials. According to the pH response type composite hydrogel dressing, gel formed by crosslinking glycyrrhetinic acid and ovalbumin amyloid fibrils is used as a carrier, and a zinc ion modified metal organic framework material is loaded on the carrier. Functional groups such as carboxyl and hydroxyl of glycyrrhetinic acid are combined with ovalbumin amyloid fibrils through hydrogen bonds and electrostatic interaction to form a hydrogel carrier, and then a zinc ion modified metal organic framework material is loaded on the hydrogel carrier to obtain the composite hydrogel dressing. The hydrogel has antibacterial and anti-biofilm effects on escherichia coli and staphylococcus aureus, has good biocompatibility and mechanical properties, has pH responsiveness, and can realize programmed control to actively remove bacteria.

The invention relates to the technical field of packaging materials, in particular to an intelligent packaging film based on a multilayer packaging nanocomposite and a preparation method of the intelligent packaging film. On the basis of the anthocyanin CD-MOFs (core) with a physical adsorption function, an ovalbumin-sodium alginate (OVA-SA) nano-composite is innovatively introduced to construct a core-shell structure, and an OVA-SA shell and a CD-MOFs pore channel form a double barrier, so that the stability of the anthocyanin is remarkably improved; the OVA-SA shell is used as an interfacial compatibilizer, and the high chemical affinity of the OVA-SA shell and a corn starch / PVA matrix is utilized, so that nano-scale uniform dispersion of MOF particles in the matrix is realized, and agglomeration is effectively avoided; meanwhile, an active pH response gated release mechanism is formed, high stability is kept in the shelf life, shell response is triggered only when a decay product induces pH rise, anthocyanin is released, color development is carried out, and sensitive monitoring on the freshness of the high-protein food is achieved.

The invention provides a nano-selenium mucosal vaccine preparation, a preparation method, application and a vaccine. The nano-selenium mucosal vaccine preparation is specifically a nano-selenium mucosal vaccine preparation which is prepared by using chicken ovalbumin (OVA) as a protein antigen and modifying nano-selenium with polyethylene glycol (PEG). The invention aims to provide a nano-selenium mucosal vaccine preparation capable of overcoming mucosal barrier, successfully presenting antigen and inducing powerful immune response and a vaccine containing the preparation.

The present application relates to the field of biological medicine, in particular to a sensitizing agent for constructing a neutrophilic asthma animal model, a construction method and application. The present application uses Haemophilus influenzae outer membrane vesicles and ovalbumin as a sensitizing agent to construct a neutrophilic asthma animal model, and the test results show that the level of neutrophils in the blood and lung lavage fluid of mice is significantly increased compared with the treatment of ovalbumin as a sensitizing agent alone. The constructed mouse neutrophilic asthma model can be used for the research on the mechanism of clinical neutrophilic asthma and the development of neutrophilic asthma drugs.

The invention discloses a magnetic drive porous spiral nano motor for active antigen delivery and a preparation method and application thereof. The magnetic drive porous spiral nano motor comprises a nickel layer, porous spiral silicon and surface antigen ovalbumin OVA, and the nickel layer modifies the surface antigen ovalbumin OVA on the surface of the porous spiral silicon. The preparation method comprises the following steps: S1, adding tetraethyl orthosilicate into a water solution containing sodium hydroxide, perfluorooctanoic acid and hexadecyl trimethyl ammonium bromide by adopting a sol-gel method, mixing, stirring and reacting, and calcining to obtain a spiral siliceous mesoporous material HR; s2, a metal nickel layer is formed on the surface of the spiral siliceous mesoporous material through magnetron sputtering, and the HR-Ni spiral magnetic drive motor is obtained; s3, surface antigen ovalbumin OVA is modified for the HR-Ni spiral magnetic drive motor, and therefore the magnetic drive porous spiral nano motor is obtained. The nano motor has high antigen loading efficiency and high cell uptake efficiency, and can enhance cross delivery of antigens.

The invention provides a traditional Chinese medicine composition for treating cough variant asthma and application thereof, and belongs to the field of traditional Chinese medicine preparations. The traditional Chinese medicine composition is prepared from radix bupleuri, herba ephedrae, herba schizonepetae, semen armeniacae amarae, radix platycodonis, fructus aurantii immaturus, exocarpium citri grandis, rhizoma phragmitis, rhizoma pinelliae, rhizoma belamcandae, the traditional Chinese medicine composition can obviously improve the condition of weight loss of guinea pigs after combined molding of ovalbumin and capsaicin, reduces the cough frequency and the lung focus area, effectively inhibits inflammatory factors, and can reach the treatment level equivalent to that of montelukast sodium.

The invention discloses a preparation method of iron-chelated ovotransferrin and an iron supplementing product thereof. The preparation method comprises the following steps: (1) pre-treating egg white: treating egg white liquid through an ultrasonic composite high-voltage pulse electric field; (2) removal of ovomucin and ovalbumin: removing the ovomucin and ovalbumin by an isoelectric precipitation method combined with a PEG precipitation method; (3) preparation of ovotransferrin; (4) separating an ovotransferrin crude product; (5) purifying an ovotransferrin crude product; (6) pre-treating the iron-chelated ovotransferrin; and (7) preparing the iron-chelated ovotransferrin: adopting an ultrasonic composite high-voltage pulsed electric field twice. The method disclosed by the invention is quick, efficient, high in ovotransferrin iron binding capacity, simple and low in cost, can be used for preparing iron-supplementing medicines and health-care foods, is beneficial to supplementing iron elements and enhancing the immunity of the organism, and is suitable for large-scale industrialization.

The invention belongs to the technical field of food processing, and relates to a preparation method and application of astaxanthin emulsion gel. The invention provides a preparation method of astaxanthin emulsion gel, which comprises the following steps: taking an ovalbumin and beet pectin compound solution obtained through electrostatic interaction under a specific pH value as a water phase, taking medium-chain fatty acid containing astaxanthin as an oil phase, and mixing the oil phase with the ovalbumin and beet pectin compound solution to obtain the astaxanthin emulsion gel. The preparation method comprises the following steps: carrying out homogenization treatment on a blend of a water phase and an oil phase by using a high-speed dispersion machine to obtain a primary emulsion, carrying out ultrasonic treatment on the primary emulsion to obtain an emulsion, adding calcium ions into the emulsion, uniformly mixing, and carrying out crosslinking and phase change on the emulsion to obtain the astaxanthin emulsion gel. According to the astaxanthin emulsion gel disclosed by the invention, the storage stability of the astaxanthin can be remarkably improved, so that more astaxanthin is migrated to a water phase in an intestinal digestion process, the bioavailability of the astaxanthin is improved, and the antioxidant activity in vivo is remarkably improved compared with that of free astaxanthin.

This invention provides a reagent kit, its preparation method, and its application, as well as a method for detecting acetochlor, relating to the fields of analytical chemistry and biosensor technology. The reagent kit includes a detection solution and a test strip; the detection solution includes CuCo@PDA conjugated with an anti-acetochlor antibody; the test strip includes a sample pad, a detection pad, and an absorbent pad arranged sequentially; from the sample pad to the absorbent pad, the detection pad has a detection line and a control line arranged sequentially; the detection line is immobilized with an ATC-BSA conjugated antigen and protein-modified ATT-AuNCs; the protein includes BSA or ovalbumin; the control line is immobilized with a secondary antibody that specifically recognizes the anti-acetochlor antibody. This reagent kit utilizes the internal filtration effect to construct a dual-mode detection mechanism of "off" colorimetric signal / "on" fluorescence signal, and the signal can be collected and analyzed via a smartphone, making it suitable for rapid on-site screening of acetochlor residues in traditional Chinese medicinal materials.

The invention discloses a construction method of an animal model for combined induction and exacerbation of asthma. The method comprises the following steps: injecting ovalbumin or dermatophagoides pteronyssinus extract into an intraperitoneal cavity for sensitization; aerosol inhalation of allergen is carried out for airway re-exposure; in the aggravating stage, pathogenic microorganisms such as pseudomonas aeruginosa, aspergillus fumigatus or respiratory syncytial virus are given and exposed to 500 mu g / m PM2.5 aerosol; lung tissue and bronchoalveolar lavage fluid are then detected. The established model shows that the airway resistance is obviously enhanced and persistent, the mucous thrombus formation rate is greater than 50%, the smooth muscle is obviously thickened, and the model is accompanied by eosinophilic granulocyte and neutrophil infiltration and high expression of various inflammatory factors. The model is good in stability and high in repeatability, can truly simulate clinical asthma acute exacerbation and steroid insensitive characteristics, and provides a platform for asthma mechanism research and new drug screening.

The invention discloses a small-molecule active aldehyde scavenger-albumin coupling medicine as well as a preparation method and application of the small-molecule active aldehyde scavenger-albumin coupling medicine. The coupling drug comprises a) the small molecule active aldehyde scavenger compound or the derivative thereof and b) albumin, and the albumin comprises at least one of human serum albumin, recombinant albumin, bovine serum albumin, ovalbumin and mouse serum albumin. The medicine is further prepared into an ophthalmic preparation, the retention time of the medicine in the cornea is prolonged, the in-vitro active aldehyde removal efficiency of the medicine is improved to 1.5 times of that of NS-2, expression (larger than or equal to 50%) of a human corneal epithelial cell inflammatory factor IL-6 is remarkably inhibited, and irritation to eyes is avoided. Through the synergistic effect of long-acting slow release of the albumin carrier and targeted removal of NS, the technical defects that a traditional xerophthalmia medicine is short in effect and single in mechanism are overcome, and a definite clinical transformation value is achieved.

The invention discloses a preparation method of a double-protein aggregate for packaging and stabilizing spice essential oil, the double-protein aggregate and application of the double-protein aggregate. According to the process, ovalbumin and lysozyme are used as raw materials, stock solutions of the ovalbumin and the lysozyme are mixed in proportion, then pH adjustment and NaCl concentration adjustment are conducted, pulsed electric field treatment is introduced, protein is promoted to be subjected to liquid-liquid phase separation under the synergistic effect to form a composite condensate, and the composite condensate is used for packaging and stabilizing spice essential oil. Through multi-dimensional synergistic regulation, the performance of the composite coagulator is remarkably improved, the embedding rate of the emulsion to the spice essential oil reaches 90% or above, the particle size increase is smaller than or equal to 10% after the emulsion is refrigerated for 6 months at 4 DEG C, the layering rate is smaller than or equal to 5%, the release rate of irritant peculiar smell components in the spice essential oil can be effectively reduced, the sensory characteristics of the spice essential oil can be greatly improved, and the flavor of the spice essential oil can be improved. The application range in the fields of food, health care products and the like is expanded.

The invention discloses an OVA-D fluorescence immunochromatography detection test strip in a surimi product and application thereof, the test strip comprises a substrate, a sample adding pad, a combination pad, a reaction film and a water absorption pad, the sample adding pad, the combination pad, the reaction film and the water absorption pad are arranged on the substrate, the reaction film is arranged in the middle of the substrate, one end of the sample adding pad is in contact with the other end of the combination pad, one end of the combination pad is in contact with the other end of the reaction film, and the other end of the reaction film is in contact with the water absorption pad. One end of the reaction film contacts with the other end of the water absorption pad; the combination pad contains a rabbit anti-OVA-D polyclonal antibody marked by fluorescent quantum dots and goat IgG marked by fluorescent quantum dots; the reaction film is provided with a detection area, the detection area is sequentially provided with a detection line and a control line from the other end to one end of the reaction film, the detection line is coated with an OVA-D antigen, and the control line is coated with a rabbit anti-goat IgG antibody. The fluorescence signal is stable, the fluorescence intensity of the T line and the C line is not influenced by a complex matrix in a detected sample, the detection sensitivity is higher, and the reliability of quantitative detection of the duck egg ovalbumin is also ensured.

The application belongs to the technical field of laser, and relates to a method for manufacturing a PVA / dye flexible random laser based on an electrostatic spinning method. A mixed solution of PVA, ovalbumin, rhodamine dye and Au nano-particles is adopted, the sample size and the morphology of the spinning structure are controlled by controlling the voltage, the spraying distance and the needle tip size in the electrostatic spinning technology process, and then the wavelength and the mode of laser emission and other parameters are adjusted. The design is simple and intuitive, the method is flexible and convenient, the principle is reliable, and the method is easy to realize. The obtained flexible random laser is easy to be coupled with other photon devices, and the quantitative control of the laser property is easy to be realized.

The invention relates to a biocompatible skin wound healing medicine and a preparation method thereof, and belongs to the technical field of biological medicine, and the medicine comprises liposome-clathrated ovalbumin peptide, a red abelmoschus esculentus seed extract, a taro stem extract, liposome-clathrated acetyl tetrapeptide-9 and vitamin E. The ovalbumin peptide is prepared by the following steps: pre-cooling ovalbumin of goose eggs, sequentially carrying out enzymolysis on the pre-cooled ovalbumin through trypsin and ginger protease, and purifying the enzymatic hydrolysate through an XAD16 nonionic macroporous resin column. The hibiscus coccineus seed extract is obtained by performing ultrasonic enzymolysis on hibiscus coccineus seeds in an ethanol water solution through hemicellulase and ginger protease and purifying enzymatic hydrolysate through an AB-8 macroporous adsorption resin column. The taro stalk extract is obtained by grinding taro stalks into pulp, performing ultrasonic extraction with an ethanol aqueous solution, and purifying an extracting solution with an HP-20 macroporous adsorption resin column. All the components are synergistically used, a multifunctional integrated repair system is constructed, and the problems that a traditional medicine is insufficient in biocompatibility, single in function and low in repair efficiency are solved.

The invention belongs to the technical field of protein peptide powder. The invention provides a preparation method of ovalbumin peptide powder, which comprises the following steps: S1, transferring protein liquid into a fermentation tank, adjusting the pH value to 6.0-6.9, adding dry yeast, stirring and fermenting to obtain fermentation liquid; s2, transferring the fermentation liquor obtained in the S1 into an enzymolysis tank, heating to 50-55 DEG C, adding a compound enzyme preparation, carrying out enzymolysis for 7-10 hours, and stirring for 10 minutes per hour to obtain enzymatic hydrolysate; the compound enzyme preparation comprises papain, subtilisin, trypsin and aminopeptidase; and S3, transferring the enzymatic hydrolysate obtained in S2 back to a fermentation tank, cooling, adding dry yeast, stirring, fermenting, spray-drying, and placing in a hot chamber for 3-7 days to obtain the product. According to the ovalbumin peptide powder, the oxidation resistance and the heat resistance are improved at the same time, so that the ovalbumin peptide powder with more excellent functionality and wider application range is obtained.

The invention discloses a method for synchronously separating ovalbumin, ovotransferrin and lysozyme from egg white, which comprises the following steps: firstly, adsorbing ovalbumin from egg white loading liquid by taking chitosan-polybutyl methacrylate nanocrystal gel with an anion exchange function as a primary separation medium, and carrying out gradient elution to obtain ovalbumin; taking a primary separation loading effluent as a secondary separation loading solution; hydroxyethyl methylacrylate-polyglycidyl methacrylate nanocrystal gel with a cation exchange function is taken as a secondary separation medium to adsorb ovotransferrin and lysozyme from a secondary separation loading solution, gradient elution is performed by gradually increasing salt concentration, and separation of ovotransferrin and lysozyme is realized. The method provided by the invention can realize synchronous separation of ovalbumin, ovotransferrin and lysozyme, has the advantages of high separation efficiency, simplicity and convenience in operation, low energy consumption and the like, and is suitable for industrial large-scale production, and the separation medium can be repeatedly used.

The invention belongs to the technical field of food microorganisms, and particularly relates to a lactobacillus paracasei BHP06 with an anti-allergic function. The preservation number of the Lactobacillus paracasei BHP06 is GDMCC (China General Microbiological Culture Collection Center) No: 66270, and the preservation number of the Lactobacillus paracasei BHP06 is CGMCC No: 66270. The lactobacillus paracasei strain provided by the invention has good gastrointestinal tract reverse environment resistance and has good survival ability in simulated gastrointestinal fluid; good self-aggregation capability and hyaluronidase inhibition capability are realized. By establishing a degranulation model of RBL-2H3 cells, it is found that the strain can inhibit mast cell degranulation and relieve allergy; by establishing an RAW264.7 cell inflammation model, the strain disclosed by the invention is found to be capable of inhibiting allergic inflammatory reaction; by establishing an OVA (ovalbumin)-induced BALB / c mouse food allergy model, the strain disclosed by the invention is found to be capable of improving the anti-allergy ability of animals and exerting the activity of relieving food allergy.

The invention discloses a preparation method of a biological ovalbumin film memristor film. The preparation method comprises the following steps: step 1, weighing egg white; step 2, adding deionized water into egg white, and fully stirring to obtain ovalbumin sol; the volume ratio of the deionized water to the egg white is (0.5-1): 1; 3, the titanium carbide MXene nanosheet with the concentration being 5 mg / ml is dispersed in deionized water, and a titanium carbide MXene nanosheet solution with the concentration being 0.005 mg / ml is obtained; step 4, mixing the ovalbumin sol and the titanium carbide MXene nanosheet solution according to a volume ratio of 1: 1, and fully stirring to obtain ovalbumin sol regulated and controlled by an MXene nanosheet layer; and 5, preparing the biological ovalbumin film memristor film by using the ovalbumin sol regulated and controlled by the MXene nanosheet layer as a raw material by adopting a dip-coating method. According to the preparation method of the biological ovalbumin film memristor film, the problem that a biological film memristor memory film is unstable is solved.

The present invention provides preparation and use of ovalbumin anti-inflammatory peptides and their Maillard reaction products. [Solution] The anti-inflammatory peptide is FGPH (Phe-Gly-Pro-His). The method for producing FGPH-MRPs, a Maillard reaction product of FGPH, is as follows: FGPH is weighed and prepared into an aqueous solution, dextran is added according to a peptide-sugar mass ratio of 1.5:1, the solution is mixed thoroughly, and the solution is reacted at 80°C and pH 9.0 for 10 hours. After the reaction is completed, the solution is cooled to room temperature to obtain FGPH-MRPs, a Maillard reaction product of FGPH. The use of FGPH-MRPs, a Maillard reaction product of FGPH, in anti-inflammatory reactions is also provided.

The invention belongs to the technical field of nano antibodies, and provides a nano antibody OVA-3-23 for resisting chicken ovalbumin and application of the nano antibody OVA-3-23. The nano antibody OVA-3-23 for resisting the chicken ovalbumin has an amino acid sequence as shown in SEQ ID NO. 1. According to the prepared nano antibody OVA-3-23 for resisting the chicken ovalbumin, in the field of directional fixation, accurate anchoring of the chicken ovalbumin is achieved with high specificity, and the defects of impurity interference and fuzzy positioning in traditional fixation are overcome; in the field of immunodetection, high specificity and strong binding force guarantee the detection accuracy, colloidal gold, enzyme and other multi-component components are matched, and the chicken ovalbumin is efficiently identified from food on-site fast screening to laboratory fine detection; in the field of enrichment and purification, a high-purity product is efficiently separated from a complex sample. Especially, reliable support is provided for application scenes such as food detection, feed quality monitoring, immunology and vaccine development, and an efficient solution is provided for detection, research and quality control related to chicken ovalbumin.