432 results about "Ovalbumin" patented technology

PCT No. PCT / SE97 / 00237 Sec. 371 Date Dec. 29, 1998 Sec. 102(e) Date Dec. 29, 1998 PCT Filed Feb. 14, 1997 PCT Pub. No. WO97 / 29825 PCT Pub. Date Aug. 21, 1997Process for separating off a peptide or a nucleic acid by an anion exchanger (I) characterized in that a) the anion exchanger (I) exhibits ligands, which (i) contain a primary, secondary or tertiary amino group and (ii) are covalently bound to an organic polymer (matrix), b) there on a carbon atom at a distance of 2 or 3 atoms away from an amino nitrogen in the ligands is a hydroxyl group or a primary, secondary or tertiary amino group, and c) the maximum elution ionic strength in the pH range 2-14 for at least one of the proteins transferrin, ovalbumin 1, ovalbumin 2, beta -lactoglobulin 1 and beta -lactoglobulin 2 on the anion exchanger is higher than the elution ionic strength required for a quaternary comparative ion exchanger.

The invention discloses a puffed minced fish instant food, which is characterized by consisting of frozen minced fish, NaCl, ovalbumin, starch, chicken extract, ginger juice, cooking wine, granulated sugar, white pepper powder, zanthoxylum powder and foaming agent, respectively by the mass percentage concentration: 60-90, 1-3, 0.5-2, 5-25, 0.5-2, 0.5-3, 0.5-3, 0.5-2, 0.01-0.1, 0.01-0.1 and 0.5-2. The preparation method comprises the steps of: unfreezing the frozen minced fish; chopping and stirring; adding with the NaCl, the ovalbumin and the starch; seasoning; adding with the foaming agent to be formed and shaped; boiling; and finally puffing by means of microwave, vacuum microwave, vacuum frying and / or vacuum drying. The puffing rate of the puffed minced fish instant food prepared by the invention is more than 2 times, thereby having crisp mouth feel, even texture, beautiful exterior appearance, and being a popular snack food.

The invention relates to a preparation method of fluorescence gold nano clusters with stable chicken ovalbumin. The method includes: dissolving commercially available chicken ovalbumin in deionized water to prepare solution 0.5-10mg / mL in concentration; mixing the solution with chloroauric acid aqueous solution to obtain mixed solution, with certain mole ratio, of the chicken ovalbumin and the chloroauric acid aqueous solution; adjusting pH of the mixed solution to 11.5-13, allowing for microwave reaction for 15-20s, and naturally cooling to room temperature to obtain fluorescence gold nano clusters emitting red light. The fluorescence gold nano clusters prepared by the method is applicable to the bio-metical field and the sensor field.

The invention provides a pharmaceutical composition of curcumin and albumin nano particles, and a preparation method of the pharmaceutical composition. The albumin is made from human serum albumin or bovine serum albumin, or is prepared by taking ovalbumin as a raw material; and preferentially, the albumin is made from human serum albumin. The pharmaceutical composition prepared by the invention has better encapsulation rate.

The invention discloses a method for preparing ovalbumin with high foamability by microwave ultrasonic waves. The method comprises the following steps of accurately weighing 50g of ovalbumin and 50g of glucose respectively, dissolving into a 500mL of 2mmol / L NaCl solution, stirring slowly at room temperature for 20 minutes until dissolving completely, mixing uniformly, adjusting the pH to 8.0, putting into a 800mL high-pressure seal tank, and modifying the ovalbumin by controlling the microwave and ultrasonic wave time as well as power so as to improve the foamability. The ovalbumin prepared by the method is high in foamability which is 6-8 times of that of the untreated protein, is simple to operate, is low in cost, is reasonable in technology, and is short in production cycle.

The invention discloses a recombinant melittin and an application thereof, and belongs to the technical field of genetic engineering. An amino acid sequence of the recombinant melittin protein is shown in the formula SEQ ID NO.1. The recombinant melittin protein sequence can be utilized for a preparation of gene medicines utilized for preventing and treating fallopian tubal diseases of breeding hens, in other words, the recombinant melittin protein sequence is connected with a carrier pVAX1 to form a recombinant melittin expression carrier. In addition, a CMV promoter of the carrier pVAX1 is replaced by a chicken ovalbumin gene end 5' regulatory sequence which is an ov sequence, thus the recombinant melittin can be expressed specifically in chicken fallopian tubal tissue. The recombinant melittin and the gene medicines have the advantages of good effects of treating fallopian tubal diseases of breeding hens, safety, no toxicity and broad application prospect.

This invention discloses one enzyme immune test method and agent case for 3- methyl quinoline 2-carboxyl acid in immune chemical analysis technique field, which comprises the following steps: processing immune antigen and cover antigen and alloantibody and pre-processing sample and establishing ELISA method; the matched agent case is composed of 3- methyl quinoline 2-carboxyl acid alloantibody covered by MQCA and egg albumin couple and MQCA standard sample; the sample is processed through metaphosphoric acid to release MQCA and MAX and indirectly competition ELISA method.

The invention discloses a composition containing oligopeptide and oligosaccharide for postoperative group to eat. The composition includes oligopeptide, oligosaccharide and traditional Chinese medicine extractives, wherein the oligopeptide is a mixture of marine collagen peptide, marine bone peptide, lactalbumin and ovalbumin; the oligosaccharide is a mixture of chitosan oligosaccharide and isomaltooligosaccharide; and the traditional Chinese medicine extractives comprise folium mori extractive, rhizoma polygonati extractive and corn stigma extractive. The composition can not only maintain nutrient balance of a postoperative patient but also promote wound healing of the postoperative patient.

The invention provides a vitamin D synthetic antigen and a preparation method thereof. The vitamin D synthetic antigen is a conjugate of vitamin D and a protein carrier. The vitamin D is 25-hydroxy vitamin D3 or 1,25-dihydroxy vitamin D3; and the protein carrier is one or more selected from bovine serum albumin, ovalbumin, hemocyanin and human serum albumin. The invention also provides application of the vitamin D synthetic antigen to vitamin D immunological detection. The invention further provides a vitamin D detection kit, which integrates the advantages of existing clinical vitamin D detection methods; and the kit can be applied to all enzyme mark instruments, chemiluminescence instruments and time-resolved analyzers, and has greatly shortened detection time, and sensitivity, accuracy and precision met the detection requirements. The immunosorbent assay kit with strong versatility provided by the invention can realize batch and rapid detection on vitamin D in serum (or plasma).

The invention discloses an antibody preparation method through which various organic phosphorus pesticide residues can be detected and the application, which belongs to the technical filed of agriculture. The antibody preparation method adopts the steps that firstly, O,O-diethylthiophosphoryl chloride is used to react with 4-aminobutyric acid to synthesize into general hapten O, O-diethyl-N-(3-carboxy-propyl)-by-sulfur Phosphoramidate DENP which comprises various organic phosphorus pesticide common structures-O, O-diethyl thiosulfate phosphoryl; secondly, the general hapten O, O-diethyl-N-(3-carboxy-propyl)-by-sulfur Phosphoramidate DENP is respectively coupled with bovine serum albumin BSA and chicken ovalbumin OVA by adopting carbodiimide method, to prepare general immunogen DENP-BSA and general coatingen DENP-OVA; and thirdly, multi-clonal antibody is prepared through immunized New Zealand white rabbits, the obtained antibody has obvious specificity to various organic phosphorus pesticides, such as thimet, demeton, disulfoion, omethoate, phoxim and quinalphos and can be used for the immune detection of the organophosphorus pesticides.

A conjugate of norfloxacin is prepared from norfloxacin semi-antigen and the bovine serum albumin (or egg albumin) as the carrier able to generate immunogenicity through coupling. It can be used for preparing the reagent kit for the enzyme-linked immunoassay of norfloxacin.

A nano colloidal golf linked immunoassary for detecting carbofuran includes such steps as coupling the immunized semi-antigen of carbofuran with BSA, synthesizing artificial immunoantigen compound, immunizing animal to prepare specific high-valence BFNH antibody, separating and purifying, linking the nano-class colloidal golf to said specific antibody, solidifying said linked compound, carbofurancoupled by egg albumin and sheep's mouse IgG antibody on a carrier, and for semi-quantitative analysis. Its advantage is high sensitivity and speed.

The invention discloses a piece of fluorescence immunoassay chromatography test paper which is rapid, sensitive, simple and convenient to operate to test the residual quantity of cefalexin, and preparation of the test paper. The test paper comprises a sample pad, a binding pad, a nitrocellulose membrane and a water absorbing pad, which are adhered to a substrate in a mutual lap joint manner in sequence, wherein the nitrocellulose membrane is coated with a detection line and a quality control line; the binding pad is coated with a cefalexin monoclonal antibody with a fluorescent mark. The preparation method of the fluorescence immunoassay chromatography test paper for cefalexin residue comprises the following steps: synthesizing cefalexin-ovalbumin coating antigen, preparing a goat anti-mouse immune globulin antibody, coating the cefalexin-ovalbumin coating antigen and the goat anti-mouse immune globulin antibody onthe nitrocellulose membrane to serve as the detection line and the quality control line, preparing a fluorescence nano-particle mark cefalexin monoclonal antibody, then coating glass fiber to serve as the binding pad, sequentially adhering the sample pad, the binding pad, the nitrocellulose membrane and the water absorbing pad to a back plate in the lap joint manner in sequence, cutting the obtained test paper into the width of 4mm, and preserving at normal temperature.

This invention relates to hydrochloric acid CLB enzyme immunity test reagent box and its test method adopting ELISA competition to test CLB and using CLB and OA coupler as CLB antigen immunity rabbits to small mouses to get multiclonal containing CLB antibody or monoclonal CLB antiserum then to be put on enzyme specimen board after separation, purification and dilution to be warm cultivated, washed, added with sample diluent solution and enzyme specimen antigen to be reacted, washed and added with enzyme substrate to display color. It is an easy test method with simple pretreatment especially for testing remaining CLB of meat food.

The invention discloses an enzyme-linked immunoassay kit for acid orange II, which belongs to the technical field of enzyme-linked immunosorbent analysis. Reagents in the kit provided by the invention comprise an enzyme label plate coated by a coupled substance of the acid orange II serving as a coating antigen and a carrier protein, standard solution of the acid orange II, solution of enzyme labeled goat-anti-rabbit antigen, antibody solution of the acid orange II, luminous solution, washing solution, coating solution and closed solution. The carrier protein of the coupled substance is bovine serum albumin or egg albumin with a molecular weight of 6.7 to 6.8 KDa. The coating antigen of the coated enzyme label plate is prepared by coupling the acid orange II and egg albumin, and an artificial immunogen for preparing the angiten is prepared by coupling the acid orange II and the bovine serum albumin. The maximum acid orange II detection range of the kit is 0.1 to 10 ng / mL. The enzyme-linked immunoassay kit of the invention has the characteristics of simplicity, quickness, accuracy and high sensitivity and can play an important role in the acid orange II detection of synthetic non-edible pigment in foods (such as marinated products and cayenne pepper) and drinks.

The invention relates to a pesticide and veterinary drug multi-residue detection method based on a microarray detection chip. The method comprises the steps that a micromolecular pesticide and veterinary drug hapten is coupled with bovine serum albumin to prepare an immunizing antigen; a mouse is immunized by the immunizing antigen to prepare a corresponding pesticide and veterinary drug monoclonal antibody; a biochip sample application instrument for a monoclonal antibody acquisition probe performs sample application on an agarose modified slide solid phase carrier to prepare a multi-repeat monoclonal antibody acquisition probe microarray; the pesticide and veterinary drug hapten is coupled with ovalbumin to prepare a hapten-OVA (ovalbumin) couplet; a fluorescent molecule Cy3 is then used for labeling; sample liquid to be detected is incubated with the chip after mixed with a labeling detection antigen according to a certain concentration; a to-be-detected antigen in a sample and the corresponding labeling detection antigen are directly and competitively bound with the corresponding monoclonal antibody acquisition probe fixed on the chip; elution is performed under a certain condition; and a result is detected by a biochip scanner. The method can detect multiple pesticide and veterinary drug residues in the subsidiary agricultural product sample simultaneously, and is applicable to high-throughput, quick and accurate detection of drug residues in planting and culture production.

Methods and systems for detecting allergens using mass spectrometry are provided herein. In some aspects, a sample can be screened for the presence or quantity of ovalbumin, lysozyme, casein (isoform S1 and S2), lactoglobulin, high and low glutens, wheat, rye, oats, barley, mustard, sesame, and various types of nuts including macadamia, pistachio, brazil, walnuts, peanuts and hazelnuts by detecting one or more peptides specific to the allergen of interest using selected MRM transitions.

The invention relates to the field of biological pharmacy, and particularly discloses a quadrivalent subunit influenza vaccine and a preparation method thereof. Used virus seeds are respectively of an H1N1 type (NYMC X-179A), an H3N2 type (NIB-88), a BY type (B / Phuket), and a BV type (NYMC BX-35). The preparation method comprises the following steps: performing chick embryo cultivation on the virus seeds; preparing a mono-valent virus stock solution by the steps of allantoic fluid collection, clarification, concentration, purification and the like; and proportioning four mono-valent virus stock solutions with different serotypes according to certain dilution points to prepare a quadrivalent subunit influenza vaccine sample. A relatively low content of ovalbumin and relatively low residual quantities of formaldehyde and a cracking agent in the quadrivalent subunit influenza vaccine sample ensure that adverse reactions of a quadrivalent subunit vaccine are lower, and the safety of the vaccine is improved. Meanwhile, compared with an existing trivalent vaccine, the quadrivalent subunit vaccine additionally has a BV serotype, thereby having a broader protection scope for popularity of influenzas with different serotypes.

The invention provides a colloidal gold cassette for rapid detection of melamine, the colloidal gold cassette has rapid detection, convenient carry and simple operation, the invention also provides a preparation method of the cassette, the preparation process thereof is simple, and the cost is low. The colloidal gold cassette comprises a test strip, the test strip is packaged in a shell body of the cassette, a sample adding hole and an observation hole are arranged on the shell body of the cassette, the test strip comprises a bottom plate, the colloidal gold cassette is characterized in that water absorbent paper, a nitrocellulose membrane, a gold-labeled pad and a sample pad are sequentially arranged on the bottom plate of the test strip, the water absorbent paper and the gold-labeled pad are respectively arranged at the two ends of the nitrocellulose membrane, small sections of the water absorbent paper and the gold-labeled pad are respectively overlapped on the nitrocellulose membrane at the junctions at the two ends of the nitrocellulose membrane, the sample pad is arranged at the other end of the gold-labeled pad, a small section of the sample pad is overlapped on the gold-labeled pad; a gold-labeled anti-melamine monoclonal antibody is sprayed on the gold-labeled pad; a detection line with the material of a melamine-ovalbumin coupled conjugate and a quality control line with the material of goat anti-mouse IgG are sequentially sprayed on the nitrocellulose membrane.

The invention relates to a preparation method of a benzothiostrobin antigen and antibody and an application thereof, belonging to the technical field of immunochemical analysis. The chemical name of benzothiostrobin is 2-[[(5-methoxy-2-benzothiazole)-thiomethyl]-alpha-(E)-methoxymethylene]methyl phenylacetate. Under an alkaline condition, carboxylic ester in the benzothiostrobin structure is hydrolyzed to synthesize an artificial hapten of which the chemical name is 2-[[(5-methoxy-2-benzothiazole)-thiomethyl]-alpha-(E)-methoxymethylene]phenylacetic acid; the artificial hapten is coupled with bovine serum albumin and ovalbumin respectively to prepared an artificial antigen. The BALB / c female mouse is immunized by the artificial antigen to obtain a specific monoclonal antibody of benzothiostrobin. The antibody does not experience a cross reaction with other compounds. An enzyme linked immunosorbent assay method established by use of the antibody can be applied to quick, sensitive, convenient and cheap detection of benzothiostrobin residues in environment and agricultural products.

The invention discloses a purification method applicable to manufacturing food-grade ovalbumin on a large scale. The purification method includes steps of collecting egg white at the room temperature, diluting the egg white according to a volume ratio of the egg white to fresh deionized water being 1:2 without adjusting pH (potential of hydrogen) of the egg white, uniformly stirring the egg white and then centrifuging the egg white to obtain supernatant; adding polyethylene glycol with the molecular weight ranging from 200 to 20000 into the supernatant obtained by means of centrifuging, uniformly stirring the supernatant and the polyethylene glycol and then centrifuging the supernatant and the polyethylene glycol to obtain high-purity ovalbumin liquor with the polyethylene glycol; carrying out ultra-filtration treatment on the high-purity ovalbumin liquor with the polyethylene glycol to manufacture high-purity ovalbumin without the polyethylene glycol. The purification method has the advantages that the purification method is simple, convenient and speedy and is low in cost and applicable to large-scale industrial production, and the food-grade ovalbumin can be manufactured under mild and safe conditions, are extremely high in quality and can be conveniently directly processed to obtain various foods, healthcare products and medical products in follow-up procedures.

The invention relates to a test paper strip for rapidly detecting traces of chlorothalonil and a preparation method thereof. The test paper strip is characterized in that a supporting layer is used as the bottom layer, an adsorption layer is used as the intermediate layer, a protective layer is fixed on the adsorption layer, the adsorption layer comprises an adsorption fibrous layer, a gold-labeled antibody fibrous layer, a cellulose film and an absorbent material layer at the handle end in order from a test terminal, wherein the cellulose film is provided with detection blots printed by using a chlorothalonil coupling carrier protein solution and control blots printed by using a goat anti-mouse IgG or rabbit anti-mouse IgG (or goat anti-rabbit IgG) antibody solution; the gold-labeled antibody is a colloidal gold labeled chlorothalonil monoclonal antibody or polyclonal antibody; and the chlorothalonil coupling carrier protein is bovine serum albumin, chicken ovalbumin or haemocyanin. The test paper strip disclosed herein has the advantages of strong specificity, high sensitivity, simple, and accurate detection, low cost, wide application scope, and easiness in popularization and application.

The invention relates to a clothianidin antigen, an antibody and an application thereof, and belongs to the technical field of immunochemistry analysis. The clothianidin antigen and the antibody of the invention are specially used for clothianidin specific polyclonal antibody preparation, enzyme-linked immunoassay (ELISA), and high-sensitivity and rapid detection of clothianidin residues in environment and agricultural products. A hapten is synthesized by substituting a chlorine atom on a thiazole ring of clothianidin, has a chemical name of 3-(5-((3-methyl-2-nitroguanidine)-yl methyl)thiazole-2-mercapto)propionic acid, and is coupled with bovine serum albumin and ovalbumin to prepare an antigen and an envelope antigen. Newzealand white rabbit is immunized by the immunizing antigen to obtain the specific polyclonal antibody of clothianidin. The established ELISA linear scope is 1.1 microgram / L-2 mg / L, and the detection limit is 1.1 microgram / L. The hapten synthetic technology of the invention is simple and feasible, high in antibody specificity, and suitable for detection and on-site monitoring of mass samples in environment and agricultural products.

The invention discloses an enzyme-linked immunosorbent assay method for multi-residue analysis of a cyano-containing pyrethroid pesticide, belonging to the technical field of immunoassay. The enzyme-linked immunosorbent assay method uses a coupled complex of hapten m-phenoxy benzal cyanohydrin succinate ester and ovalbumin as a coating antigen, samples of the cyano-containing pyrethroid and a polyclonal antibody of the m-phenoxy benzal cyanohydrin succinate ester are added, then the coating antigen and the cyano-containing pyrethroid to be detected are competed and reacted with the antibody, an enzyme-labeled secondary antibody is added to be combined with the fixed antibody, washing liquid is used for washing, developer is added, an enzyme-labeled instrument is used for detecting OD value, the OD value is compared with a standard curve of a standard product of the cyano-containing pyrethroid, and the concentration of the cyano-containing pyrethroid of the samples to be detected is calculated. The enzyme-linked immunosorbent assay method can accurately and sensitively detect the residues of the cyano-containing pyrethroid pesticide in the samples to be detected, the pre-treatment process of the samples is simple with less time-consuming, a large number of samples can be simultaneously detected, and the detection cost of the samples is far lower than the detection method of the traditional instrument; furthermore, the method of the invention has good stability and no radioactive pollution.

The invention relates to a one-step ELISA (Enzyme Linked Immunosorbent Assay) method for neomycin (NEO) residues in milk, which belongs to the technical field of immunoassay chemicals. The method comprises the following steps of: coupling NEO with bovine serum albumin (BSA) or ovalbumin (OVA) to synthetize a NEO antigen (immunogen NEO-BSA or coating antigen NEO-OVA) by a glutaraldehyde method; authenticating the NEO-BSA by utilizing an infrared spectroscopy and an SDS-PAGE (Sodium Dodecyl Sulfate-PolyAcrylamide Gel Electrophoresis) method; obtaining NEO antiserum by immunization and detectingthe valence of the NEO antiserum by an indirect ELISA method; and detecting the specificity of the NEO antiserum by the one-step ELISA method. Compared with the indirect competition ELISA method, theinvention greatly shortens the detecting time of the specificity of the NEO antibody, has higher sensitivity, achieves the aim of simply, rapidly and sensitively detecting NEO residues in milk and can be widely used for rapid field detection.

The invention discloses a reagent kit for the chemiluminescence enzyme-linked immunoassay of furazolidone, which comprises a kit body, an enzyme label plate arranged in the kit body and a reagent arranged in the kit body, wherein, holes of the enzyme label plate are coated by an envelope antigen which is manufactured through coupling between 3-azyl-2-oxazolone that is a metabolin of furazolidone and ovalbumin; and the reagent contains a polyclonal antibody of furazolidone, an enzyme label secondary antibody that is a goat anti-rabbit antibody marked by horseradish peroxidase, a furazolidone-series standard solution, a concentrated phosphate buffer solution, a concentrated cleaning solution and a luminescent liquid. The reagent kit has the characteristics of high sensitivity, good reproducibility, simplicity, convenience, speediness and accuracy; compared with the traditional color comparison ELISA method, the sensitivity can be improved by one order higher, and the reagent is expected to play an important role in furazolidone residue detection of water used in stock raising, feeds and animal-derived foods (such as milk samples, animal tissue samples and urine samples).

A probiotic lactobacillus was discovered from lactobacilli of the Lactobacillus genus independently isolated from human adult feces. The probiotic lactobacillus was selected from other bacterial strains for: (1) being highly resistant to gastric acid / bile acid; (2) having a high promoting activity on IL-12 production from mouse derived spleen cells and a high Th1 / Th2 balance-improving effect; (3) having a high ability to inhibit the production of antigen-specific IgE induced by intraperitoneally administering ovalbumin to BALB / c mice; (4) having a high ability to inhibit the production of antigen-specific IgE induced by orally administering a food antigen to C57BL / 6N mice; (5) having a high Natural Killer cell-activating ability; (6) having a high IL-12 production-promoting activity on spleen cells and mesenteric lymph node cells derived from mice immunized with ovalbumin and a high Th1 / Th2 balance-improving effect; and (7) having a high ability to suppress eosinophilia induced by a cedar pollen-extracted antigen. This discovery led to the completion of the present invention.

The invention discloses a chemiluminescent enzyme-linked immunosorbent assay (CELISA) kit for detecting ractopamine. The kit comprises a kit body, in which an ELISA plate with wells coated with coating antigen prepared from ractopamine and ovalbumin by coupling, a series of ractopamine standard solutions, enzyme-labeled goat anti-rabbit antibody, anti-ractopamine antibody, a chemiluminescence solution, a washing solution, a coating solution and a blocking solution are arranged. The CELISA kit is sensitive, simple, rapid and accurate; and the sensitivity is improved by one order of magnitude compared with that of the conventional colorimetric ELISA method. The CELISA kit can be used for detecting residual ractopamine in animal-derived foods (such as milk and animal tissues) and urine samples.

The invention discloses a chemical luminescence enzyme-linked immunoassay kit of gatifloxacin, comprising a kit body, an enzyme-marked plate and a reagent which are arranged in the kit body; wherein, all the holes of the enzyme-marked plate are coated with coating antigen; the coating antigen is prepared by the coupling of the gatifloxacin and ovalbumin; the reagent comprises a polyclonal antibody of the gatifloxacin, an enzyme-marked second antibody (namely, the antibody of horseradish peroxidase-marked goat anti-rabbit), a gatifloxacin series standard solution, a concentrated phosphate buffer, a concentrative cleaning solution and a luminescence solution. The kit has the advantages of high sensitiveness, good repeatability, simpleness, fastness and exactness; and compared with traditional colorimetric ELISA method, the sensitiveness can be improved by one magnitude and the method is expected to play an import role in the gatifloxacin residual detection of animal source foods (such as milk samples and animal tissue samples) and a urine sample.

The invention discloses an ofloxacin coupling compound with general formula (I), which comprises coupling compounds of ofloxacin hapten and bovine serum albumin of carrier substance which can produce immunogen or egg albumin. Thereinto, n stands for molecular number of ofloxacin combined with a bovine serum albumin molecule, the said n is an integer between one and twenty, and BSA stands for bovine serum albumin with 6.6KDa-6.9KDa of molecular weight. The invention also discloses a process for preparing the said coupling compound, which consists of joining ofloxacin and carrier substance which can produce immunogen to obtain the said coupling compound which induces immune system of animals to produce antibody. By immuning white rabbits from New Zealand, the ofloxacin coupling compound of this invention has prepared antiserum with 1:512,000 of potency, of which the lowest check limit is 0.1ppb. The invention is characterized in that it is simple, rapid, specific, exact and so on, which provides a foundation for preparing enzyme immunoassay agent box of ofloxacin.