2351results about How to "Improves antioxidant activity" patented technology

An antioxidant composition having enhanced oxidative stability, emulsion stability, and health benefits. The composition may include individual ingredients or a synergistic blend of non-reducing sugars, sugar polyols, medium-chain triglycerides, polysaccharides, polyphenols, phospholipids, chitosan, and alpha-casein, beta-casein, kappa-casein or protein fragments, glycopeptides, phosphopeptides. The composition may optionally be further utilized for the prevention of hypercholesterolemia or bone mineral loss.

The present invention relates to the modified release of nutritional supplements by combining one or more isolated and purified long-chain polysaccharides and one or more nutritional supplements selected from anti-oxidants, vitamins, minerals, amino acids, nucleic acids, mixtures and combinations thereof, wherein the supplement is compressed at a pressure greater that 100 psi.

The preparation methods of globefish skin active collagen and the peptide of the active collagen are that the globefish skin is cut into small pieces to be frozen, dried, crushed and put into sodium chloride solution to be stirred for 12 hours and then for centrifugation to discard supernatant and remove non-collagen proteins, deposition is put in to acetum, pepsin is added under 0 DEG C to 10 DEG C for enzymolysis and centrifugation, the supernatant is put through an ultrafiltration membrane, and the above part is frozen and dried to obtain the collagen. After the active collagen is prepared, the rest deposition is centrifugated until the pH value is between 2.0 to 7.0, papain is added into the deposition for the enzymolysis, the supernatant is centrifugated and respectively put through the ultrafiltration membrane after enzyme-denaturing to obtain the globefish skin collagen peptide of different molecular weight ranges, and the globefish skin collagen peptide are frozen and dried to obtain the light yellow or white powdery globefish skin collagen active peptide which has good antioxidation character and takes effects of eliminating free radical and reducing the senescence degree.

The invention discloses an extraction method of anthocyanin and original anthocyanin components in cranberry fruits, which comprises the following steps: crushing the cranberry fruits, enzymolyzing the crushed fruits with biological enzyme, and lixiviating with water under the condition of the pH value being in the range of 1.0-5.5 to obtain water extracting solution and residues; carrying out alcohol extraction on the residues, concentrating the obtained extracting solution and recovering alcohol to obtain alcohol extraction concentrated solution; and merging the water extracting solution with the alcohol extraction concentrated solution, adsorbing with macroporous absorption resin, washing the macroporous absorption resin with water, analyzing with water-containing alcoholic solution, and concentrating and drying the obtained analytical liquid to obtain a mixture containing the anthocyanin and original anthocyanin components. The extraction method of the invention has the advantages of simple process, strong equipment generality, small investment, high yield, low cost and applicability to large-scale production; and the obtained mixture can be used as a raw material of foods, healthcare products and medicines or an additive.

The invention relates to blood lipid-lowering active fractions of forsythia suspensa and a preparation method and use of the same. The active fractions are ethyl acetate fractions obtained by concentrating an extract which is extracted from forsythia suspensa leaves by acetone and water. The compound of phillygenin prepared by the invention and the active fractions, namely the ethyl acetate fractions, at which the phillygenin is positioned have good antioxidant activity in vitro. In-vivo experiments show that the ethyl acetate fractions and the phillygenin can lower total cholesterols and low-density lipoprotein cholesterin in the blood serum of a mouse with hyperlipidemia and can lower blood lipid level by an oxidative approach (raising superoxide dismutase SOD and lowering malonydialdehyde MDA). In addition, the ethyl acetate fractions have a better effect of reducing the total cholesterols in the blood serum of the mouse than the phillygenin and cassia seed blood lipid-lowering tablets. The ethyl acetate fractions of the forsythia suspensa leaves can be further developed to play a role in the multi- target co-prevention and treatment of hyperlipidemia and other diseases and have a good application prospect.

The invention relates to an extraction method of an active substrate, and in particular relates to a method for extracting an active substance from the sea cucumber processing waste. By taking the viscera of sea cucumber as a raw material, the method is used for preparing refined sea cucumber polysaccharide through the processes of pretreatment, enzymolysis, reflux extraction, microwave extraction, alcohol precipitation, decoloration, centrifugation, salt precipitation, dialysis, refining and the like; the supernate after alcohol precipitation is subjected to the processes of rotary evaporation, extraction degreasing, water saturation n-butyl alcohol extraction, rotary evaporation, macroporous adsorption resin chromatography and the like to obtain refined sea cucumber total saponins; and the aqueous solution after the water saturation n-butyl alcohol extraction is processed by the technologies of decoloration, sterilization and the like to obtain antioxidant active sea cucumber polypeptide. The method provided by the invention turns the waste of sea cucumber viscera into wealth so as to obtain three products including sea cucumber polysaccharide, sea cucumber total saponins and antioxidant active sea cucumber polypeptide.

The invention relates to whey protein antioxidant peptides, a preparation method thereof and application thereof. In the method, whey protein, which is a cheese by-product, and an enzymatic preparation undergo enzymatic hydrolysis to form a whey protein antioxidant peptide zymolyte, the zymolyte is subjected to static or dynamic primary purification by macroporous absorption and separation and purification by gel filtration chromatography, preparative high-performance liquid chromatography and analytical high-performance liquid chromatography to give pure whey protein antioxidant peptide products, and a polypeptide amino acid sequence is measured. The product has high digestion resistance, heat resistance, acid resistance and alkali resistance, high storage stability, low molecular weightand specific amino acid sequence, and can effectively remove free radicals, stop oxidation reaction and reduce the generation of oxidation damages, so the products have a wide application prospect inthe field of food, medicaments, feed and cosmetics.

The invention discloses a preparation method of oligomeric proanthocyanidins. The preparation method comprises the following steps of: adding tea polyphenols to a procyanidine plant raw material or an extract thereof; dissolving the procyanidine plant raw material or the extract into an acid aqueous solution according to percentage concentration by weight; carrying out reaction on a mixture for 0.5-5 h at 40-100 G C; adsorbing the solution after reaction by an adsorbing resin; washing the solution after reaction by a distilled water to remove impurities; eluting the solution after reaction by ethanol water; and concentrating and drying the solution after reaction so as to obtain the oligomeric proanthocyanidins. The preparation method of the oligomeric proanthocyanidins, disclosed by the invention, has the advantages of high oligomeric proanthocyanidin content, low average degree of polymerizatoin, high water solubility, high recovery rate, environment friendliness, high efficiency, simplicity and convenience in operation and low cost, can be applied to the fields, such as foods, healthcare foods, drugs, cosmetics, daily chemicals, animal feeds and the like, and is suitable for large-scale industrial production.

The invention relates to the technical field of biology, and discloses an oyster peptide with a sexual promoting function and a preparation method and application thereof. The method comprises the steps that oyster meat, calcium salt and water are ground to obtain oyster meat paste; enzymolysis is performed on the oyster meat paste, and centrifugation is performed to obtain supernatant after enzymolysis is performed, and an oyster enzymolysis coarse solution is obtained; discoloring and impurity removing are performed on the oyster enzymolysis coarse solution, and an oyster peptide refined solution is obtained; concentrating and spray drying are performed on the oyster peptide refined solution to obtain the oyster peptide. According to the oyster peptide with the sexual promoting function and the preparation method and application thereof, due to the fact that the oyster meat is pretreated with the calcium salt before enzymolysis is performed on the oyster meat, endogenous enzymes of oysters are activated, and enzymic preparations consumed in subsequent enzymolysis are reduced; meanwhile, the specific enzymic preparation composition is adopted to cooperate with the endogenous enzymes of the oysters to perform enzymolysis in two steps, and therefore the oyster peptide with the higher antioxidant activity and the significant sexual promoting function is prepared.

The invention relates to Isopentenyl-oxyl substituted dehydrogenized silybin ether and the preparation method and the application thereof. The compound has the activity of eliminating extracorporeal extracorporeal 1-diphenyl-2-picrylhydrazyl radical, the activity of obviously inhibiting the generation of lipid peroxide induced by radical and the function of powerfully preventing the damage of therat adrenal pheochromocytoma PC12 cells due to the hydrogen peroxide, i.e. the function of preventing the oxidative damage of the PC12 cells simulating the brain nerve cells, thereby playing a positive role in preventing the brain cell tissue oxidation and brain never oxidation and preventing and treating the senile dementia and other neurodegenerative diseases. The pharmacodynamic result shows that 7-site substituted dehydrogenized silybin ether can be expectantly used for preparing the medicament for preventing and treating the neurodegenerative diseases.

The invention relates to a gel preparation for maintaining the activity of cryopreserved cells and a stem cell gel preparation containing stem cells. The gel preparation comprises the following components by the mass percentage: 1-3% of sodium alginate, 1-5% of dimethyl sulfoxide, 1-5% of propylene glycol, 1-5% of epigallocatechin gallate, 2-10% of dextran, 1-5% of human blood albumin, and water or a phosphate solution added to make the total amount be 100%. The gel preparation has the advantages of good biocompatibility and simple operation, can maintain high activity of cells, can be directly used for low temperature preservation, and has no need of traditional liquid nitrogen cryopreservation; the cell gel preparation after resuscitation can still maintain high activity of the cells and dryness of the cells, and can be applied for treatment of skin injury or mucosal injury.

The invention relates to a formula of an aquatic product composite preservative. The formula of the aquatic product composite preservative is characterized in that the aquatic product composite preservative in each liter of distilled water comprises the following components: 0.4 to 0.6 g of muramidase, 2.5 to 3.5 g of tea polyphenol, 16 to 20 g of carboxymethyl chitosan, 10 to 14 g of nisin, 25 to 35 g of potassium sorbate, 17 to 23 g of sodium alginate, 20 to 30 g of lactobacillus, 1.5 to 2.5 g of propolis extracts and 10 to 14 g of spice extracts; and the spice extracting liquid comprises the raw materials: galangal, garlic, onion, cinnamon, clove and rosemary. The aquatic product composite preservative provided by the invention is prepared by compounding biological preservatives, is safe and non-toxic, has simple preparation and application methods, prominent microbial inhibition effect and good preservation effect, can greatly prolong the shelf life of frozen aquatic products, and has important industrial value.

The invention discloses a skin-whitening composition as well as a preparation method and applications of the skin-whitening composition. In the skin-whitening composition, various whitening agents and sun-screening agents with excellent properties are matched for use, so that the melanin formation can be inhibited, moreover, ultraviolet rays can be absorbed, and free radicals can be eliminated. The skin-whitening composition is prepared from radix puerariae extract, glutathione, kojic dipalmitate, ascorbyl glucoside, nicotinamide, beta-hydroxy acid, undecylenoyl phenylalanine, chamomile extract, green tea extract, licorice root extract, pomegranate bark extract, 1,3-butanediol, phenoxyethanol and water. The components are mixed to achieve the synergistic skin whitening effect, the skin whitening effect is obvious, safe and steady, and no toxic and side effects are caused.

The invention relates to a method for extracting anthocyanin from plant berries and particularly relates to a preparation method of acanthopanax sessiliflorus berry anthocyanin with antioxidant activity. The preparation method comprises the following steps of (1) selecting acanthopanax sessiliflorus berries as raw materials; (2) extracting the acanthopanax sessiliflorus berry anthocyanin by using an enzyme freezing and thawing method: mixing the frozen raw materials and an extracting solution, unfreezing and breaking the frozen raw materials, and carrying out enzymolysis; (3) carrying out ultrasonic-microwave synergistic extraction on the acanthopanax sessiliflorus berry anthocyanin: carrying out ultrasonic-microwave synergistic extraction on enzymatic hydrolysate, and centrifuging to obtain a crude extracting solution; (4) purifying the crude extruding solution of the anthocyanin: absorbing the crude extruding solution by using macroporous resin, collecting an eluant, and carrying out vacuum concentration; (5) freezing and drying in vacuum; and (6) smashing the anthocyanin, and then, packaging the anthocyanin. The preparation method has the advantages of high extracting efficiency, short time, low energy consumption, small reagent dosage, stable properties of effective components and the like; and meanwhile, a vacuum freeze drying technology is adopted, so that a product has better biological activity, color, luster and solubility and the preparation method is suitable for industrial production.

The invention discloses a whey protein active peptide with antioxidant activity and a preparation method thereof. The preparation method comprises the following steps: (1) adding protease into whey protein solution for enzymolysis, wherein the enzymolysis temperature is 40-60 DEG C, and the pH value is 6.0-8.0; (2) after enzymolysis, inactivating the protease; (3) precipitating the whey protein not hydrolyzed in enzymolysis solution, and removing the whey protein to take supernatant; (4) carrying out ultrafiltration on the supernatant by an ultrafiltration membrane the cut-off molecular weight of which is less than or equal to 10,000 dalton, collecting filter liquor of peptide the molecular weight of which is less than or equal to 10,000 dalton; (5) concentrating the filter liquor; (6) desalting the concentrated liquor; and (7) concentrating the desalted solution, and then freezing and drying. In the invention, the obtained whey protein active peptide has significant antioxidant property and specific functional characteristics and stable quality, and can be widely applied to the fields of food, cosmetics, medicine and the like. The production technology is simple and is convenientfor scale production, the raw material whey protein has high use ratio, and the production cost is low.

The invention discloses a 4-carbamate-3-methoxy cinnamic acid cyclamine alkyl amide compound, and a preparation method and application thereof. The compound prepared in the invention has low toxic and side effect, and can treat neurodegenerative diseases including, but not limited to, vascular dementia, the Alzheimer's disease, the Parkinson's disease, the Huntington's disease, HIV-associated dementia, multiple sclerosis, amyotrophic lateral sclerosis, neuropathic pain, glaucoma and the like.

The invention discloses an antioxidant peptide of hairtail fish protein as well as a preparation method and uses of the antioxidant peptide. In particular, hairtail fish is used as raw materials; an enzymatic hydrolysis solution is obtained through degreasing and enzymolysis of protamex and neutral protease; the enzymatic hydrolysis solution is separated and purified through ultra-filtration, purification of macroporous resins, cation exchange resin chromatography, gel column chromatography, and reversed-phase high-performance liquid chromatography so as to obtain the antioxidant peptide Asn-Trp-Asp-Met-Glu-Lys-Ile-Trp, and the ESI-MS determining molecular weight is 1121.25da. The high-activity antioxidant peptide disclosed by the invention has a good scavenging effect on DPPH free radicals, hydroxyl radicals and superoxide anion free radicals; besides, the antioxidant peptide shows a good effect of lipid peroxidation inhibition. The antioxidant peptide has the advantages of safe use, zero toxic or side effects, high oxidative activity and the like, is liable to digest and absorb, and can be used as medicines, health food or food additives, and the like.

The invention discloses a method for comprehensively extracting various kinds of biologically active ingredients from mushroom dry powder; the technical proposal is as follows: firstly, extracting alcohol soluble substances; secondly, extracting mushroom proteins; thirdly, extracting water soluble polysaccharide of mushroom, fourthly, extracting alkali extraction water-insoluble polysaccharide and alkali extraction water soluble polysaccharide; fifthly, preparing mushroom fiber. The extracted constituents have different biological activities, for example, the alcohol soluble substances have strong antioxidation activity; the mushroom proteins are rich in essential amino acid; the lentinan has antineoplastic activity; and the mushroom fiber has the physiological function of a dietary fiber. Therefore, the extracted constituents can be used respectively.

The invention relates to a method for extracting and preparing medlar leaf flavone. The method comprises the following steps of: (1) after picking and removing impurities manually, drying medlar leaves indoor to obtain a medlar leaf dry sample; (2) crushing the medlar leaf dry sample and screening the crushed medlar leaf dry sample to obtain medlar powder; (3) leaching the medlar powder by using ethanol to obtain leaching liquor; (4) grinding the leaching liquor by using a colloid grinder to obtain colloidal medlar leaf leaching solution; (5) homogenizing and separating the colloidal medlar leaf leaching solution to obtain liquid supernatant and filter residue; (6) removing impurities from the liquid supernatant by using a ceramic membrane to obtain filtrate; (7) adsorbing the filtrate by using weak-polarity macroporous adsorption resin and eluting the filtrate by using ethanol solution to obtain medlar leaf flavone-containing eluent; and (8) after concentrating and drying the medlar leaf flavone-containing eluent under a reduced pressure in a vacuum, obtaining the medlar leaf flavone powder. The method has low cost and high extraction rate; and the obtained medlar leaf flavone has stable properties, high content, and high biological activity of heart head blood-vessel disease resistance, cancer resistance, oxidation resistance and the like.

The invention discloses a method for preparing an oxidation resistance product from rosemary and belongs to the technical field of preparing an oxidation resistance product with high activity. The preparation steps include: the dry leaves of rosemary are cracked and screened; the essential oil of the rosemary is extracted by adopting a vapor distilling method; the residues obtained after extracting the essential oil are filtered for removing the remained water; then the same organic solvent is adopted for repeated leaching; a leaching liquid is filtered, decompress-vaporized and dried in vacuum for obtaining the coarse extract of the rosemary which contains the oxidation resistance active components; the coarse extract is absorbed by macroporous resins and chromatography is carried out on the coarse extract by a silica gel column; cut fractions are collected and dried in vacuum for obtaining the oxidation resistance product of rosemary. In the product prepared by the invention, the contents of the main oxidation resistance active components of sage acid and sage phenol are high; the content sum is larger than 50wt percent; moreover, the oxidation resistance activity of the product is high, thus being beneficial to industrial production and the application in the market.