25results about How to "Improves antioxidant activity" patented technology

This invention discloses an optimized extraction and purification process for total flavonoids from Hypericum perforatum and its application in photodamage protection. Belonging to the field of natural product extraction technology, the process includes the following steps: using Hypericum perforatum powder as raw material, ultrasonic-assisted extraction is performed, followed by centrifugation, collection of the supernatant, and repeated extraction of the residue twice. The supernatants are combined to obtain a total flavonoid extract from Hypericum perforatum. The extract is then purified by adsorption using a macroporous adsorption resin, and the eluent is collected, concentrated, and dried to obtain high-purity total flavonoids from Hypericum perforatum. This invention features a stable process, high extraction efficiency, and is environmentally friendly, making it suitable for industrial production. The prepared total flavonoids from Hypericum perforatum exhibit high purity and significant antioxidant and photoprotective effects, and can be widely used in antioxidant, skin protection, and sunscreen products.

The application provides a method for improving the flavor and quality of tilapia surimi products, and belongs to the technical field of aquatic product processing, and comprises the following steps: tilapia muscles are cut into small pieces, an improving agent is added, and the muscles are ground; the well-stirred surimi is made into fish cakes and shaped; the fish cakes are taken out after floating, and are placed in cold water for cooling; the shaped fish cakes are respectively fried at 150 DEG C, 180 DEG C and 210 DEG C for 4 min; the fish cakes are vacuum packaged and stored in a refrigerator after cooling; the flavor of the surimi is analyzed, the quality of the surimi is detected, and the deterioration of the surimi is determined and analyzed; threonine, creatinine and glucose are dissolved in a phosphate buffer to obtain A liquid; diethylene glycol is taken, mixed with soybean oil, and mixed with an improving agent by vortexing to obtain B liquid; the A liquid and the B liquid are fully mixed in a 48 mL sealed high-temperature pressure-resistant reaction bottle, and then are placed in an oil bath for reaction and then are placed in an ice water bath for reaction termination; the solution after reaction is removed and is reserved; the MeIQx content, precursor substances and intermediate substances of the MeIQx generation simulation system solution after reaction simulation are determined.

The application belongs to the technical field of medicine, and particularly relates to a composition for alleviating and repairing testicular damage caused by heat stress and application thereof. The composition for alleviating and repairing testicular damage caused by heat stress comprises the following raw materials: lycopene inclusion compound, ginsenoside Re, modified chitosan nanoparticles and a solvent. The amount of the modified chitosan nanoparticles is 10-15 wt% of the ginsenoside Re. The application innovatively designs a combination formula with lycopene and ginsenoside Re as the core. Both of the two main effective components in the formula are natural plant or traditional Chinese medicine extract, which effectively avoids the conflict between the medicinal properties of traditional Chinese medicines and has good biological safety. The composition can significantly repair the testicular damage caused by heat stress and accelerate the recovery after heat stress damage.

The application provides a synthesis method and application of silk fibroin-based melanin-like nanoparticles, and belongs to the technical field of nanoparticle preparations for biomedical use. After mixing silk fibroin nanoparticles, tyrosine and tyrosinase solution, silk fibroin-based melanin-like nanoparticles are obtained through one-pot copolymerization, and the enzyme concentration in the tyrosinase solution is 200-250 U / mL. The synthesis method has the advantages of simple operation, green environmental protection and the like, and the synthesized silk fibroin-based melanin-like nanoparticles have excellent antioxidant activity, ultraviolet protection and significant anti-skin cell photoaging.

This invention discloses a fermentation process for a prickly pear and sea buckthorn compound fruit wine, belonging to the field of fruit wine fermentation technology. The invention includes the following steps: selecting prickly pear juice and sea buckthorn juice, mixing them in a 2:1 volume ratio as fermentation raw materials; adjusting the pH of the juice to a suitable range, adding sugar and preservatives, and adjusting the initial sugar content; activating yeast and adding it to the juice in a specific ratio, stirring evenly; placing the inoculated juice into a fermentation container and fermenting for 9 days under constant temperature conditions; filtering to remove sediment after fermentation to obtain primary fruit wine; clarifying the wine by low-temperature settling to promote impurity sedimentation; using moderate heating for sterilization to ensure product safety and stability; bottling after all indicators pass testing, and sealing and storing the finished product. This invention, by optimizing the compound ratio of prickly pear and sea buckthorn and the synergistic fermentation process parameters, can achieve an effective fusion and balance of the two raw materials in terms of flavor, nutrition, and fermentation kinetics.

The application belongs to the field of biological medicine, and particularly relates to a sulfur-containing prodrug compound and medical use thereof. 1‑6 alkyl, substituted C 1‑6 alkyl, C 6‑10 aryl, substituted C 6‑10 aryl; R2 is selected from H or C 1‑6 alkyl, substituted C 1‑6 alkyl; R3 is selected from H or C1-6 alkyl, substituted C 1‑6 alkyl; R4 is selected from H, COOH or COOR 5 ; wherein R 5 is selected from C 1‑6 alkyl, substituted C 1‑6 alkyl or benzyl; L is selected from or a chemical bond; wherein X is selected from H, halogen, C 1‑6 alkyl, CH3O, CN or NO2; X is single substitution or multiple substitution at any position; Y is selected from O, S, NH or NCH3; R6 is selected from H or C 1‑3 alkyl; R7 is selected from H or C 1‑3 alkyl; n is selected from 1, 2, 3 or 4.

This invention relates to the food field and discloses a compound plant product, characterized in that the product comprises jujube extract, soybean peptide, corn oligopeptide, artichoke extract, amla, jujube seed, and optional additives; on a dry basis, the weight ratio of the jujube extract, soybean peptide, corn oligopeptide, artichoke extract, amla, and jujube seed is 1:(0.03-0.5):(0.03-0.5):(0.003-0.02):(0.005-0.05):(0.005-0.05); the tannin content in the product is 0-0.5 mg / g dry basis weight. The compound plant product provided by this invention, through the specific combination of the above components, exhibits higher antioxidant activity, better DPPH scavenging ability, and better ABTS scavenging ability; per gram dry basis, the tannin content is low, with minimal astringency and bitterness, resulting in a superior flavor.

This invention proposes a method for the biosynergistic extraction and refined utilization of all active ingredients in ginseng, belonging to the fields of medical, dental, or cosmetic formulations. This invention utilizes ginseng stress extract obtained through specific microbial treatment. This method leverages the stress stimulation of *Pseudomonas fluorescens* to efficiently synthesize various saponins, polysaccharides, and volatile oils in ginseng, resulting in an extract rich in components and with enhanced activity. Combining fractional extraction, resin purification, and nanoemulsification processes, a skincare raw material with comprehensive components, high stability, and excellent bioavailability is ultimately obtained, exhibiting a powerful synergistic effect in anti-inflammatory and anti-aging properties.

This invention belongs to the field of food technology, specifically relating to a fermented tomato cube and its preparation method. The preparation method includes the following steps: (1) Peel and seed fresh tomatoes, cut them into cubes, sterilize them, and place them in a sterile fermentation container. First, add *Lactobacillus casei*, mix evenly, and anaerobic ferment. Then, add *Lactobacillus rhamnosus*, mix evenly, and continue anaerobic fermentation. After sterilization, fermented tomato cubes are obtained; (2) Wash and peel onions, cut them into cubes, and pre-cook them to obtain pre-cooked onion cubes; (3) Mix the fermented tomato cubes, pre-cooked onion cubes, and seasonings evenly, cook at 80-85℃ for 20-25 minutes, and cool to room temperature to obtain fermented tomato cubes. The preparation method of this invention can ensure the efficient increase of total phenols in fermented tomato cubes while reducing the degradation of lycopene, achieving synergistic optimization of the nutritional quality of fermented tomato cubes, and improving the sensory quality of the final product.

This invention discloses a lutein composition for relieving eye fatigue and its preparation method, belonging to the field of functional foods. The process involves: reacting phycocyanin and kelp polyphenols to obtain modified phycocyanin; reacting fucoidan and β-glucuronidase to obtain modified fucose; reacting fish collagen peptides and zinc chloride to obtain collagen peptide-zinc chelate; reacting lutein silica cage complex and modified phycocyanin to obtain a first complex; reacting the first complex and modified fucose to obtain a second complex; reacting the second complex, collagen peptide-zinc chelate, and krill oil to obtain a lutein complex; reacting the second complex, collagen peptide-zinc chelate, and krill oil, then adding trehalose to obtain the lutein complex; and finally mixing the lutein complex with maltodextrin to obtain the lutein composition.

The application belongs to the technical field of microcapsule preparation, and discloses a lutein microcapsule with high stability and antioxidant activity and a preparation method thereof. In the application, protein is dissolved, then Tagetes erecta polyphenol solution and lutein solution are added, and after sufficient stirring for a certain time, polysaccharide solution is added, pH is adjusted, and magnetic stirring is carried out for 1-2 hours, and then lutein microcapsule powder is obtained by freeze-drying. In the application, the lutein microcapsule is prepared by using Tagetes erecta polyphenol, protein and polysaccharide, so that the stability of lutein is improved, the antioxidant activity of lutein is enhanced, and the prepared Tagetes erecta polyphenol composite lutein microcapsule has high application value in the fields of food and medicine.

The present application relates to the technical field of functional microorganism screening and application, and particularly relates to a Lactobacillus plantarum with antioxidant and antibacterial functions and application thereof.The Lactobacillus plantarum is Lactobacillus plantarum WW strain, which has been preserved in the China General Microbiological Culture Collection Center on December 24, 2021, and the preservation number is CGMCC No.24189.The strain has strong acid and bile salt tolerance, outstanding antioxidant and antibacterial effects, and can be widely applied in the fields of food or medicine and the like.

This invention discloses a Bacillus subtilis ( Bacillus subtle The EB-8 strain, deposited on January 14, 2026, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37320, is disclosed. The application of Bacillus subtilis strain EB-8 in the fermentation modification of tartary buckwheat flour is also disclosed. This invention's Bacillus subtilis strain EB-8 can significantly increase the amylose content of tartary buckwheat, helping to inhibit starch retrogradation and improve its freeze-thaw stability; simultaneously, it increases the total flavonoid and total phenolic content of tartary buckwheat, enhancing its antioxidant activity. Furthermore, this strain can selectively retain and enrich essential amino acids such as lysine and methionine, reduce bitter components, improve taste, and facilitate the formation of a cereal matrix suitable for cold processing, with a mild taste and enhanced oxidative defense. Overall, it achieves synergistic optimization of the functional activity and processing characteristics of tartary buckwheat flour, which is beneficial for the high-value utilization of tartary buckwheat flour resources.

The present application belongs to the field of microbial application and feed processing technology, and discloses a bacillus subtilis, named bacillus subtilis YT1, which is preserved in China Center for Type Culture Collection, located at Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, with a preservation number of CCTCC NO: M 20242767 and a preservation date of December 10, 2024. The present application also discloses a microbial preparation and the application of the bacillus subtilis or the microbial preparation in the processing of silage. The present application first simultaneously possesses high efficient lignocellulose degradation ability, excellent fermentation promotion characteristics, significant antioxidant activity and good probiotic characteristics in a strain of bacillus subtilis. This characteristic enables a single bacterial agent to achieve multiple goals such as fiber degradation, fermentation promotion, oxidation protection, which traditionally requires multiple compounded bacterial agents, and fundamentally overcomes the problems of poor compatibility between strains, unstable synergistic effect and high application cost of existing compounded additives.

This invention discloses a novel use of Artemisia annua seeds, a medicinal and edible compound composition for relieving hangovers and protecting the liver, chewable tablets, and their preparation process. This invention is the first to discover that the mucilage in Artemisia annua seeds slows down ethanol diffusion through a dual mechanism of physical adsorption and three-dimensional network blockage, thus slowing down the rate of ethanol absorption in the digestive tract, reducing the peak blood plasma concentration, and delaying or prolonging the time of intoxication. Therefore, it can be used in the preparation of health products / functional foods that protect the stomach, prevent intoxication, and delay alcohol absorption. Based on this discovery, this invention provides a medicinal and edible compound composition for relieving hangovers and protecting the liver, composed of 10-15 parts by weight of Pueraria lobata, 10-15 parts by weight of Hovenia dulcis, 8-10 parts by weight of Lilium brownii, 4-8 parts by weight of Poria cocos, 4-8 parts by weight of Dioscorea opposita, and 4-8 parts by weight of Artemisia annua seeds. Furthermore, to balance rapid effectiveness and compliance, it is formulated as a chewable tablet. This chewable tablet can significantly prolong the time of intoxication and shorten the sobering-up time, and is significantly superior to the group lacking Artemisia annua seeds in terms of antioxidant indicators (SOD, GSH, MDA) and hangover relief effect.

This invention discloses a method for preparing Yuanjiang asparagus extract and its application. The method uses fresh Yuanjiang asparagus tender stems as raw material. After selection, washing, freeze-drying, pulverizing, and sieving, choline chloride and 1,2-propanediol eutectic solvent are added for constant-temperature extraction. After ultrasonic temperature control, cellulase and pectinase are added for complex enzymatic hydrolysis. After heating to inactivate the enzymes, centrifugation is performed, and the supernatant is collected to obtain the crude extract of Yuanjiang asparagus saponins. This method has high extraction efficiency, and the obtained asparagus saponins have strong antioxidant activity, exhibiting strong scavenging ability against DPPH, ABTS, and hydroxyl radicals, as well as Fe... 2+ The chelation rate was high, and it showed significant in vitro inhibitory effects on the proliferation of human colorectal cancer cells Caco-2 and human hepatocellular carcinoma cells HepG2. The raw material used in this method is fresh, tender stems of Yuanjiang asparagus, which is widely available and of excellent quality. The extraction process is green, environmentally friendly, and simple, not only improving the comprehensive utilization value of Yuanjiang asparagus but also providing a new method for extracting natural saponin active ingredients. The obtained asparagus saponins can be used to develop antioxidant and anti-tumor functional foods, showing potential market prospects.

This invention provides a method for extracting chlorogenic acid, a coffee byproduct. Using fresh coffee peel and coffee grounds as raw materials, chlorogenic acid is extracted using ethanol extraction and ultrasound-assisted extraction. Through single-factor and orthogonal experiments, the average chlorogenic acid content in the extract from fresh coffee peel under the conditions of 50% ethanol concentration, 60 min of ultrasound, 40℃ ultrasound temperature, and a solid-liquid ratio of 1:12 (g / mL) was 0.075 mg / mL. The average chlorogenic acid content in the extract from coffee grounds under the conditions of 50% ethanol concentration, 6 h of extraction time, and a solid-liquid ratio of 1:15 (g / mL) was 0.059 mg / mL. Antioxidant activity assays showed that the chlorogenic acid extracted from both coffee byproducts exhibited good antioxidant activity. This invention not only reduces the waste of coffee byproducts but also realizes their deep processing and high-value utilization, which is conducive to promoting the sustainable development of the coffee industry.

This invention discloses an alkaline protease mutant and its application in the preparation of active hippocampal peptides. This alkaline protease mutant exhibits high enzyme activity and high thermal stability, with an optimal operating temperature of 60°C. Even after treatment at 70°C for 90 minutes, it retains 23.45% of its relative enzyme activity, demonstrating excellent thermal stability. When used for the enzymatic hydrolysis of defatted hippocampal powder, it can increase the yield of active hippocampal peptides from 11.7% to 82.4%, and improve the antioxidant activity (IC50) of the hydrolysate. 50 The enzyme activity was increased by approximately 51.4%, effectively solving the bottleneck of the difficulty in synergistically improving enzyme activity and thermal stability in existing technologies. This provides an excellent catalyst and process solution for the industrial-scale, efficient, and low-cost production of hippocampal active peptides.

This invention discloses a method and application for extracting soluble dietary fiber from soybean residue using a pulsed electric field assisted extraction. The method includes dissolving dried, pulverized, and sieved soybean residue powder in distilled water to obtain a soybean residue liquid; subjecting the soybean residue liquid to repeated freeze-thaw cycles followed by pulsed electric field treatment; adding a compound enzyme to the pulsed electric field-treated soybean residue liquid for enzymatic hydrolysis; after enzymatic hydrolysis, inactivating the enzyme, centrifuging to collect the supernatant, and then concentrating, precipitating with alcohol, and freeze-drying the supernatant to obtain soluble dietary fiber from soybean residue. This invention employs a synergistic extraction process of repeated freeze-thaw cycles, followed by a pulsed electric field, and then compound enzymatic hydrolysis, which can effectively improve the extraction rate of soluble dietary fiber from soybean residue. The obtained soluble dietary fiber from soybean residue exhibits excellent antioxidant activity and pancreatic lipase inhibitory activity, while its physicochemical properties are significantly improved, making it suitable for use in functional foods and food additives, with outstanding industrial application value.

This disclosure relates to the field of pharmaceutical excipients and additives, specifically a method for preparing a pharmaceutical sweetener. The method includes the following steps: preparing modified microalgae polysaccharides; adding the modified microalgae polysaccharides, a first sweetener, a second sweetener, dietary fiber, and an acidity regulator to water, mixing thoroughly, and then spray-drying to obtain the pharmaceutical sweetener. This disclosure solves the problem of poor palatability of microalgae polysaccharides; enabling microalgae polysaccharides, as a filler sweetener, to be compounded with high-intensity sweeteners, other filler sweeteners, dietary fiber, and acidity regulators, thereby possessing both good tableting performance and antioxidant activity.

This invention provides a prickly pear and Job's tears yogurt and its preparation method, belonging to the field of yogurt preparation technology. The preparation method of the prickly pear and Job's tears yogurt includes the following steps: soaking Job's tears and mixing them with water, then successively pulping, gelatinizing, and then hydrolyzing with α-amylase at high temperature to obtain a Job's tears enzymatic hydrolysate; inoculating the Job's tears enzymatic hydrolysate with probiotic BLH1 seed liquid for fermentation; after fermentation, adding prickly pear juice to the fermentation liquid for further fermentation to obtain a prickly pear and Job's tears fermentation liquid; mixing milk powder, water, and sucrose evenly, homogenizing, sterilizing, cooling, and then mixing evenly with the prickly pear and Job's tears fermentation liquid, inoculating with lactic acid bacteria seed liquid for fermentation, and after fermentation, ripening to obtain the prickly pear and Job's tears yogurt. The prickly pear and Job's tears yogurt provided by this invention has a good taste, high sensory evaluation, significantly retains functional components such as phenols, flavonoids, and vitamin C, and greatly increases GABA content and antioxidant activity.

This invention discloses a DNA barcode, primers, and their applications for screening high-quality Tibetan brown mushrooms. The DNA barcode for screening high-quality Tibetan brown mushrooms comprises one or more of the 17 DNA fragments with nucleotide sequences as shown in SEQ ID NO: 1-17; the DNA barcode amplification primers for screening high-quality Tibetan brown mushrooms comprise one or more pairs of the 17 primer pairs with upstream and downstream nucleotide sequences as shown in SEQ ID NO: 18-51. Compared with traditional breeding methods and other existing DNA barcoding technologies, this invention has the advantages of being time-saving, labor-saving, cost-effective, accurate, and efficient, playing a positive role in the genetic breeding of high-quality Tibetan brown mushrooms, and also providing an effective method for the identification and protection of germplasm resources.

This invention belongs to the field of biomedical technology, specifically disclosing a method for extracting and applying fermented sword bean oil. The method involves first activating Bacillus subtilis, then inoculating it onto sword bean powder for solid-state fermentation. After drying and pulverizing the fermented material, extraction is performed using an extraction solvent on a shaker and followed by filtration. The filtrate is then evaporated under vacuum to remove the solvent, yielding fermented sword bean oil. This method disrupts the cell walls of sword beans, promoting oil release and increasing the oil yield. After fermentation, the content of polyunsaturated fatty acids in the oil increases to 25.763±0.663%, with the addition of pentadecenoic acid and erucic acid, as well as DPPH and ABTS. + The free radical scavenging ability and iron ion reducing ability are significantly enhanced, resulting in improved antioxidant activity. The process conditions of this invention are mild, the process is simple, the energy consumption is low, and it is easy to scale up. The resulting oil can be used in the food, health product, and pharmaceutical industries.

The application belongs to the technical field of marine chemical engineering, and particularly relates to N,N,N-trimethyl chitosan quaternary ammonium salt containing glycine Schiff base, a preparation method and application thereof. The N,N,N-trimethyl chitosan quaternary ammonium salt containing glycine Schiff base is shown in formula (1). The application has the advantages of simple reaction equipment, easy control of reaction process, low cost of raw materials, high safety, easy popularization and industrial application. Research proves that the compound has good antioxidant activity and bacterial inhibition activity, and can be applied to the fields of agriculture, medicine and environmental protection.

The application provides a method for preparing fish skin collagen peptides with high antioxidant activity by DHPM and UIO-66 molecular imprinting, and relates to the technical fields of aquatic product deep processing and bioactive peptides. The method comprises the following steps: fish skin pretreatment, DHPM-induced collagen protein unfolding, endogenous enzyme autolysis, crude separation, selective target enrichment by using UIO-66 material with histidine as a molecular imprinting template, and drying collection. The application destroys the dense triple helix structure of collagen protein by using the physical field effect of dynamic high pressure microfluidization, exposes the enzyme cutting site, uses the endogenous enzyme of fish skin for autolysis, and then uses the histidine molecular imprinting UIO-66 material to accurately capture the high antioxidant activity peptide segment, so that the enzymolysis efficiency and the antioxidant activity of the product are significantly improved. The application does not need to add exogenous enzymes, and has the advantages of green process, low cost and high product purity. The obtained fish skin collagen peptides can be widely applied to the fields of functional food, cosmetics and medicine.