103results about How to "Improves antioxidant activity" patented technology

The invention discloses lactobacillus fermentum with a hypoglycemic function and application of the lactobacillus fermentum. According to the invention, lactobacillus fermentum with high hypoglycemic ability is screened from Yunnan Baoshan freshly brewed yoghurt samples, the strain is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20241504. In the fermentation process of the strain, alpha-glucosidase and alpha-amylase can be inhibited, and the inhibition rates are 43.91% and 59.44% respectively. The bacterial colony morphology of the lactobacillus fermentum E003 is milky white, semitransparent, relatively moist and smooth, the edge is neat, and obvious bulges are formed. When being used for preparing the anti-sugar fermented milk, the fermented milk can also inhibit alpha-glucosidase and alpha-amylase, and meanwhile, the fermented milk also has various characteristic nutritional ingredients; the lactobacillus fermentum E003 fermentation liquor also has relatively strong bacteriostatic ability, antioxidant activity and GABA high-yield ability.

The invention discloses a preparation method of a multifunctional polyvinyl composite film and application of the multifunctional polyvinyl composite film in food preservation. The preparation method comprises the following steps: firstly, preparing Ag-coated MOF through a hydrothermal method, then uniformly dispersing the Ag-coated MOF and tannic acid into a carboxymethyl chitosan solution, and carrying out crosslinking modification on the Ag-coated MOF; and coating the prepared CMCS / TA / Ag (at) MOF mixed solution on a polyethylene film treated by oxygen plasma, and drying to form a film, so as to obtain the multifunctional polyethylene-based composite film. The CMCS and the TA are adopted to perform crosslinking modification on the Ag-coated MOF, so that the stability of the Ag-coated MOF can be further improved, and the release of silver ions is reduced. Compared with a common PE preservative film, the composite film prepared on the basis has better barrier capacity and mechanical property, and has antibacterial, anti-ultraviolet and anti-oxidation effects. The multifunctional preservative film is convenient to use and low in price, and has an important application prospect in the aspect of fresh-keeping packaging of meat products.

The invention relates to nutrient-rich red yeast rice bran and a preparation method thereof.The preparation method comprises the following steps that after rice bran is subjected to enzyme deactivation and sterilization treatment, the moisture is controlled to be 20%-30%; adding a composite enzymatic hydrolysate into the pretreated rice bran, uniformly stirring, and performing enzymolysis to obtain a semi-solid base material; the temperature of the semi-solid base material is controlled to be 36-40 DEG C, and the pH value is adjusted to be 5.0-5.5; inoculating monascus for culturing, turning over the yeast once every 10-12 hours, and culturing for 5-6 days to obtain a yeast material; spreading and culturing the koji material, and controlling the temperature of the koji material to be 30-35 DEG C; after the culture is completed, drying the yeast material until the moisture is below 10% to obtain the nutrient-rich red yeast rice bran; the monascus rice bran with high content of monacolin K can be obtained through the method, the technological operation is simple, the labor intensity of workers is reduced, the production period is short, and the method is suitable for large-scale application and has remarkable economic benefits and social benefits.

The invention provides a biodegradable food preservative film as well as a preparation method and application thereof. The preservative film is a chitosan and polyvinyl alcohol composite film loaded with cinnamon oil microcapsules. The preparation method mainly comprises microcapsule preparation and film preparation. Wherein the microcapsule preparation comprises the following steps: dissolving beta-cyclodextrin, adding cassia oil, and stirring the mixture to form a microcapsule emulsion. And storing the emulsion in a refrigerator overnight. And on the second day, filtering the solution to collect a precipitate, washing and drying to obtain the microcapsule. The film preparation comprises the step of mixing a polyvinyl alcohol solution, a chitosan solution and glycerol according to a fixed proportion. Then, adding cassia oil microcapsules, and uniformly stirring to obtain a film-forming solution; and pouring the film forming liquid into a mold flat plate to prepare a film, thereby obtaining the antibacterial composite film. The tear strength of the prepared film is 21.18 N / mm < 2 > to 43.21 N / mm < 2 >, the elongation at break is 82.60% to 147.56%, and the antioxidant activity is 27.47% to 36.87%; the preservative has an obvious inhibiting effect on staphylococcus aureus and escherichia coli, and can effectively prolong the shelf life of fresh-cut fruits.

The invention belongs to the field of fermentation, and particularly relates to kluyveromyces marxianus, a complex microbial inoculant, fermented tea primary pulp as well as a preparation method and application of the kluyveromyces marxianus. Wherein the Kluyveromyces marxianus is Kluyveromyces marxianus Linzhi.KM-D3, the Kluyveromyces marxianus Linzhi.KM-D3 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the Kluyveromyces marxianus Linzhi.KM-D3 is CCTCC NO: M 20231575. The DPPH free radical scavenging capacity of the fermented tea raw stock obtained through fermentation of the kluyveromyces marxianus is larger than or equal to 140%, the hydroxyl free radical scavenging capacity is larger than 130%, the ABTS free radical scavenging capacity is larger than 80%, and the SOD enzyme activity of each L of the fermented tea raw stock is larger than 1300U. Meanwhile, the pH value of the fermented tea puree provided by the invention is less than or equal to 4.0, the total acid content (in terms of lactic acid) is greater than or equal to 6g / 100g, the ethanol content is less than or equal to 0.5 g / 100g, the sucrose content is less than or equal to 0.5 g / 100g, and the fermented tea puree has no fermentation peculiar smell and light bitter taste in the aspect of sensory evaluation.

The invention relates to the technical field of compound fruit wine fermentation, and particularly discloses a compound fruit wine fermentation process for enhancing flavor quality and antioxidant activity by using plant lactobacillus, which comprises the following steps: (S01) taking concentrated apple juice, and diluting with purified water; (S02) mixing and compounding the Huyou full juice with the diluted apple juice in the step (S01); (S03) adjusting the pH value of the compounded mixed solution in the step (S02) to 4-5; (S04) inoculating special yeast for fruit wine into the compound mixed solution obtained in the step (S03); (S05) inoculating the compound mixed solution obtained in the step (S04) with plant lactobacillus; and (S06) fermenting the inoculation liquid obtained in the step (S05) to obtain a composite fruit wine finished product. The fruit wine has the characteristics of being relatively good in flavor diversity, high in health value and good in sensory flavor quality.

The invention discloses halomonas for efficiently synthesizing nano-selenium as well as a method for preparing the nano-selenium and application of the halomonas, and belongs to the technical field of biology. The Halomonas sp. CCTCC NO: M 20242266 disclosed by the invention has strong tolerance to high-concentration selenite, can efficiently convert the selenite into nano-selenium, and is simple in required culture condition, low in cost and easy to operate; the nano-selenium particles produced by utilizing the strain disclosed by the invention are stable in property and high in biological activity. The Halomonas sp. CCTCC NO: M 20242266 or a microbial agent of the Halomonas sp. CCTCC NO: M 20242266 or nano-selenium produced by utilizing the strain has wide development prospects in the fields of functional agriculture including planting industry and breeding industry, functional food processing, health food processing, medical product processing and the like.

The invention discloses a procyanidine compound hard capsule for a health food capable of maintaining beauty and keeping young. The procyanidine compound hard capsule is prepared from the following components: a procyanidine extract, chitosan, vitamins, collagen, hyaluronic acid, a lotus leaf extract, astragalus polysaccharide, casein phosphopeptides, D-mannitol, starch, magnesium stearate and gelatin. The invention relates to a production process and application of a procyanidine compound hard capsule for beauty and young keeping health food, and the production process comprises the following steps: step 1, adjusting a formula proportion by using a component proportion optimization model to obtain an optimal preparation effect; 2, solution preparation is conducted on all the components, and the prepared solutions are mixed together; step 3, drying the procyanidine compound solution by adopting low-temperature vacuum drying; and 4, metering the dried procyanidine compound powder by using a capsule filling machine, and sub-packaging the procyanidine compound powder into medicinal hard capsules. The beauty-maintaining, young-keeping and health-care effects are greatly improved.

The invention relates to a preparation method of a high-performance soybean protein emulsion film, which comprises the step of carrying out fibrosis denaturation on soybean protein by adopting an acetic acid-water binary solution, so that the hydrophobic property, the film-forming ability and the environmental interference resistance of the soybean protein can be remarkably improved. And further starting from a protein self-assembly fibrosis principle and a protein film forming theory, a soybean protein-based biological membrane with certain mechanical strength and flexibility is constructed through self-assembly fibrosis denaturation of protein molecules in an aqueous solution, so that the problems of poor mechanical property and high solubility of the existing protein-based biological membrane are effectively solved.

The invention provides a preparation method and application of lucid ganoderma nano powder, a lucid ganoderma polysaccharide extract or a lucid ganoderma triterpenoid extract, and belongs to the technical field of lucid ganoderma processing and preparation. The preparation method of the lucid ganoderma nano powder comprises the following steps: airing lucid ganoderma sporocarp, cutting into lucid ganoderma particles, and crushing the lucid ganoderma particles to obtain lucid ganoderma coarse powder; drying the ganoderma lucidum coarse powder at constant temperature, and performing superfine grinding on the dried ganoderma lucidum coarse powder to obtain the ganoderma lucidum powder. The ganoderma lucidum nano powder is successfully prepared through superfine grinding, the content of heavy metal in the ganoderma lucidum nano powder is low, the content of polysaccharide and the content of triterpenes measured in the ganoderma lucidum polysaccharide extract and the ganoderma lucidum triterpenes extract obtained through extraction of the ganoderma lucidum nano powder are high, and the antioxidant activity of the ganoderma lucidum polysaccharide extract and the antioxidant activity of the ganoderma lucidum triterpenes extract are high; further research finds that the ganoderma triterpenoid extract has remarkable activity of inhibiting brain glioma. Various functional products are prepared from the ganoderma lucidum nano powder, and the ganoderma lucidum nano powder has important significance and application value.

The application belongs to the technical field of polymer materials, and particularly relates to a high-performance fiber composite material and a preparation method thereof. The preparation method of the high-performance fiber composite material comprises the following steps: adding pretreated aluminum oxide whiskers into a solvent, then adding pretreated carboxymethyl cellulose and gallic acid, and heating to reflux to obtain modified aluminum oxide whiskers; then mixing epoxy resin, the modified aluminum oxide whiskers, a curing agent and an accelerator uniformly to coat a film to obtain a resin adhesive film; and compounding the resin adhesive film and carbon fibers through hot pressing to obtain carbon fiber prepreg, and heating and curing the obtained prepreg to obtain the high-performance fiber composite material. The fiber composite material obtained by the application not only has high strength and rigidity, but also has excellent toughness, can effectively resist the impact of the external environment, has oxidation resistance and long-term stability, and has a wide application prospect in the fields of aerospace and new energy equipment.

The invention discloses a preparation method of a viable bacterium type Chinese wolfberry enzyme, and belongs to the field of enzyme preparation. The preparation method comprises the following steps: inoculating lactobacillus rhamnosus and lactobacillus paracasei into Chinese wolfberry juice, and fermenting to obtain viable bacterium type fermented Chinese wolfberry juice; and adding auxiliary materials into the viable bacterium type fermented Chinese wolfberry juice, and sterilizing in a water bath to obtain the viable bacterium type Chinese wolfberry enzyme. According to the method, by optimizing the strain compounding proportion, the inoculum size, the fermentation time, the sterilization temperature of the lycium barbarum enzyme and the like, a simple and efficient method for preparing the viable bacterium type lycium barbarum enzyme is provided, and a foundation is laid for improving the potential value of lycium barbarum. After being sterilized in a water bath at the temperature of 40 DEG C, the viable bacterium type Chinese wolfberry enzyme obtained by the method can be ensured to contain high active substances such as flavones and polyphenols and have good antioxidant activity.

The invention relates to the technical field of fabrics, in particular to a long-term preservation post-treatment method for silk brocade, which comprises the following steps: step 1, preparation of a finishing agent: mixing a Chinese waxgourd peel extract, hydrogen peroxide, an ultraviolet light absorber, a nano ultraviolet finishing agent and deionized water to prepare a finishing liquid; step 2, pretreatment of the brocade fabric: performing plasma treatment on the brocade fabric; step 3, finishing the brocade fabric; the brocade fabric is put into the finishing liquid for two times of dipping and two times of rolling, the antibacterial and anti-aging brocade fabric is obtained through hot drying, the Chinese waxgourd peel extract is cooperatively matched with hydrogen peroxide, an ultraviolet light absorber and an ultraviolet finishing agent, the anti-ultraviolet and antibacterial properties of the fabric are further optimized, the service life of the silk brocade fabric is prolonged, and the silk brocade fabric is worthy of popularization and application. The technical effect that the beauty of the silk brocade fabric is kept for a long time is achieved.

The application discloses a preparation method of a hydrocolloid dressing for oral cavity, and relates to the technical field of medical supplies. Baicalin extract and a polyvinyl pyrrolidone solution are mixed to obtain a baicalin extract solution; chitosan fibers, hydroxymethyl cellulose and the baicalin extract solution are mixed to obtain a mixed solution; vacuum degassing: the prepared mixed solution is vacuumized, pressure is maintained, and primary degassing is performed; ultrasonic degassing: the mixed solution after primary degassing is subjected to ultrasonic degassing; static degassing: the solution after ultrasonic degassing is sealed by using a preservative film, and is subjected to secondary degassing at room temperature; a PU film is fixed in a film laying frame, the degassed mixed solution is poured into the film laying frame, and the film laying frame is placed into an air-blowing drying box to solidify and form a protective layer, so that the hydrocolloid dressing is obtained. The hydrocolloid dressing prepared by the method has good adhesion, can be used on a wound for a long time, and can effectively promote the repair of damaged cells and wounds, so that the healing of the wound in the oral cavity is promoted.

The present invention discloses a cultivation matrix for oyster mushrooms and its application in improving the antioxidant activity, nutritional quality and growth performance of oyster mushrooms, belonging to the technical field of fungal cultivation. The cultivation matrix for oyster mushrooms comprises the following components in mass fraction: 50% of hawthorn kernel material, 33% of cottonseed shell, 15% of wheat bran, 1% of lime and 1% of glucose; wherein the hawthorn kernel material is selected from one of hawthorn kernel powder, water-extracted hawthorn kernel, ethanol-extracted hawthorn kernel, ester-extracted hawthorn kernel, carbonized hawthorn kernel, mixed hawthorn kernel and whole hawthorn kernel. After adding hawthorn kernel to the cultivation matrix, the antioxidant activity, nutritional quality and growth performance of oyster mushrooms can be improved; especially when the hawthorn kernel is carbonized, the antioxidant activity of oyster mushrooms can be extremely significantly improved.

This invention discloses an optimized extraction and purification process for total flavonoids from Hypericum perforatum and its application in photodamage protection. Belonging to the field of natural product extraction technology, the process includes the following steps: using Hypericum perforatum powder as raw material, ultrasonic-assisted extraction is performed, followed by centrifugation, collection of the supernatant, and repeated extraction of the residue twice. The supernatants are combined to obtain a total flavonoid extract from Hypericum perforatum. The extract is then purified by adsorption using a macroporous adsorption resin, and the eluent is collected, concentrated, and dried to obtain high-purity total flavonoids from Hypericum perforatum. This invention features a stable process, high extraction efficiency, and is environmentally friendly, making it suitable for industrial production. The prepared total flavonoids from Hypericum perforatum exhibit high purity and significant antioxidant and photoprotective effects, and can be widely used in antioxidant, skin protection, and sunscreen products.

The present invention relates to the field of biotechnology, and discloses a white mullet antioxidant peptide, a preparation method and application thereof. The white mullet antioxidant peptide of the present invention is extracted from white mullet, and the amino acid sequence is shown in SEQ ID NO: 1. The preparation method comprises the following steps: first, simulating human in vitro digestion to obtain an enzymatic hydrolyzate from the white mullet, using ultrafiltration to separate ultrafiltration components with a molecular weight cutoff of less than 3000Da, and after gel chromatography, using an RP-HPLC method to further separate and purify the chromatography components with antioxidant activity to obtain the white mullet antioxidant peptide. The white mullet antioxidant peptide prepared by the present invention has a strong antioxidant effect and can be used to prepare antioxidant products.

This invention belongs to the field of bio-fermentation technology, specifically relating to a high-glycemic-lowering active mulberry leaf fermentation broth, its preparation method, and its application. The preparation method includes the following steps: mulberry leaves are pulverized and sieved at low temperature; water and a carbon source are added to prepare a fermentation substrate; a bacterial suspension of *Lactobacillus mucilaginosus* and *Lactobacillus paracasei* is prepared; and the bacterial suspension is inoculated into the mulberry leaf fermentation substrate at a volume ratio for fermentation to obtain the high-glycemic-lowering active mulberry leaf fermentation broth. This invention selects *Lactobacillus mucilaginosus* CICC21800 and *Lactobacillus paracasei* CICC6263 in a certain proportion to form a fermentation microbial community. This community consists of two bacteria with different and complementary metabolic pathways, which can form a relatively stable biological community, mutually promoting each other. This community transforms macromolecules in mulberry leaves into smaller molecules and secretes beneficial polysaccharides, proteins, and other substances, increasing the content of effective active ingredients in the fermented product and exhibiting excellent fermentation effects on mulberry leaves.

The invention discloses preparation and application of black fungus enzyme extraction polysaccharide. The preparation method comprises the following steps: S1, drying black fungus, grinding, sieving, and collecting to obtain black fungus superfine powder; s2, the black fungus superfine powder is subjected to degreasing, enzyme extraction, alcohol precipitation and drying, and the black fungus enzyme extraction polysaccharide is obtained. The black fungus enzyme extraction polysaccharide reacts with a complexing agent to prepare the black fungus enzyme extraction polysaccharide-iron compound which is used for preparing iron supplementing soft sweets. The black fungus enzyme-extracted polysaccharide iron-supplementing soft sweets are prepared by taking black fungus as a raw material, so that the product types of iron-supplementing and blood-enriching medicines and health-care functional products in China are enriched, and the existing market gap in China is supplemented.

This invention discloses an endogenous polypeptide, its preparation method, and its application. The structural formula of the polypeptide is shown in Formula (I): R1-(AA1)m-AA2-AA3-(AA4)n-R2, Formula (I); wherein, m is independently 2 or 3; n is independently 1, 2, or 3; AA1 is independently K, R, or H; AA2 is independently I or W; AA3 is independently I or W; AA4 is independently K, R, or H; R1 is any one of -H, Y, LY, PLY, or LLY; R2 is any one of -OH, L, LL, E, EL, EW, W, EH, or EHL. While retaining its targeted antioxidant and anti-inflammatory functions, the polypeptide has removed the domain with high affinity for heparin, thereby avoiding signal interference and potential biosafety risks that may be caused by full-length PF4, and meeting the safety requirements for cosmetic ingredients.

The invention relates to the technical field of organic polymer material modification and composite coating preservation, in particular to a natural antibacterial coating preservation material as well as a preparation method and application thereof. The preparation method comprises the following steps: dispersing an amphiphilic antibacterial chitosan derivative into a solvent A to obtain a water phase; adding essential oil into the water phase, homogenizing, and carrying out ultrasonic emulsification to obtain a Pickering emulsion; dissolving a film-forming material in a dispersing agent, and adding a plasticizer to obtain a film-forming base solution; and mixing the Pickering emulsion, the film-forming base solution and water, adding nano reinforced particles and a binder, homogenizing, and degassing to obtain the natural antibacterial coating fresh-keeping material. The antibacterial material is grafted in a natural polymer material chitosan molecular structure, meanwhile, the organic polymer material and the plant essential oil are compounded, the natural antibacterial coating fresh-keeping material with multiple antibacterial functions is constructed, the natural antibacterial coating fresh-keeping material is used for postharvest fresh keeping of tropical fruits, and the fresh-keeping effect is good.

This invention discloses a method for preparing resveratrol-loaded whey protein amyloid fibers based on moderate electric field modification. The method involves pretreating whey protein using a moderate electric field non-thermal effect, forming amyloid fibers through acid-thermal treatment, and further constructing a resveratrol delivery system based on protein fibrils. The resulting whey protein fiber-resveratrol complex exhibits excellent loading performance, antioxidant activity, and bioavailability, providing an effective carrier construction strategy for the efficient delivery and stabilization of hydrophobic and environmentally sensitive active molecules. This invention reveals the regulatory mechanism of the moderate-intensity electric field non-thermal effect in the protein fibrillation process, providing new technical ideas and theoretical references for the precise design of protein fiber structures and the development of functional protein-based delivery carriers, and providing technical support for the green preparation of protein fibrils and their in-depth development in functional foods.

The invention discloses low-caffeine anti-aging fermented tea and a preparation method thereof, and belongs to the technical field of tea processing. According to the invention, an Aspergillus Tennesensis strain is obtained by screening, and the preservation number of the Aspergillus Tennesensis strain is CGMCC (China General Microbiological Culture Collection Center) No.42415. The method comprises the following steps: inoculating aspergillus Tennesensis into a culture medium only containing tea leaves and water, fermenting, and airing after aspergillus Tennesensis hyphae completely cover the tea leaves, so as to obtain the low-caffeine anti-aging fermented tea. The aspergillus Tennesensis is used for degrading caffeine in the tea leaves, the growth speed is high, the degradation efficiency is high, and the degradation rate of the caffeine can reach 98% or above after 20 days of fermentation; the fermented tea leaves have very excellent antioxidant activity. The method for preparing the fermented tea is high in operability, the prepared fermented tea is red and dark in tea soup, black brown, mellow, smooth and low in bitterness degree, and the flavor quality of the tea leaves is improved.

The application provides a method for improving the flavor and quality of tilapia surimi products, and belongs to the technical field of aquatic product processing, and comprises the following steps: tilapia muscles are cut into small pieces, an improving agent is added, and the muscles are ground; the well-stirred surimi is made into fish cakes and shaped; the fish cakes are taken out after floating, and are placed in cold water for cooling; the shaped fish cakes are respectively fried at 150 DEG C, 180 DEG C and 210 DEG C for 4 min; the fish cakes are vacuum packaged and stored in a refrigerator after cooling; the flavor of the surimi is analyzed, the quality of the surimi is detected, and the deterioration of the surimi is determined and analyzed; threonine, creatinine and glucose are dissolved in a phosphate buffer to obtain A liquid; diethylene glycol is taken, mixed with soybean oil, and mixed with an improving agent by vortexing to obtain B liquid; the A liquid and the B liquid are fully mixed in a 48 mL sealed high-temperature pressure-resistant reaction bottle, and then are placed in an oil bath for reaction and then are placed in an ice water bath for reaction termination; the solution after reaction is removed and is reserved; the MeIQx content, precursor substances and intermediate substances of the MeIQx generation simulation system solution after reaction simulation are determined.

The application belongs to the technical field of medicine, and particularly relates to a composition for alleviating and repairing testicular damage caused by heat stress and application thereof. The composition for alleviating and repairing testicular damage caused by heat stress comprises the following raw materials: lycopene inclusion compound, ginsenoside Re, modified chitosan nanoparticles and a solvent. The amount of the modified chitosan nanoparticles is 10-15 wt% of the ginsenoside Re. The application innovatively designs a combination formula with lycopene and ginsenoside Re as the core. Both of the two main effective components in the formula are natural plant or traditional Chinese medicine extract, which effectively avoids the conflict between the medicinal properties of traditional Chinese medicines and has good biological safety. The composition can significantly repair the testicular damage caused by heat stress and accelerate the recovery after heat stress damage.

The invention discloses a preparation and regeneration method and application of phellinus igniarius protoplast. The method comprises the following steps: inoculating a phellinus igniarius strain to an improved PDA culture medium for activation, inoculating vigorously-growing hypha blocks to an improved PDB culture medium for culture, washing hyphae with sterile water, and absorbing surface moisture to obtain wet phellinus igniarius hyphae; carrying out enzymolysis on the wet phellinus igniarius hyphae in a dark place by using lywallzyme and driselase to obtain phellinus igniarius hypha enzymatic hydrolysate; filtering the phellinus igniarius hypha enzymatic hydrolysate, centrifuging the filtrate, washing the precipitate with a mannitol solution to obtain purified phellinus igniarius protoplast, and dispersing the purified phellinus igniarius protoplast in the mannitol solution to obtain a phellinus igniarius protoplast suspension; and diluting the phellinus igniarius protoplast suspension with a mannitol solution, and coating the diluted phellinus igniarius protoplast suspension on an improved PDA regeneration culture medium plate for regeneration culture. The phellinus igniarius protoplast suspension is subjected to ultraviolet ray and lithium chloride compound mutagenesis, the protoplast after mutagenesis is subjected to regeneration culture, and the phellinus igniarius dominant mutant strain with the hypha growth speed, the mycelium biomass, the flavone yield and the mycelium ethanol extract antioxidant activity improved is obtained through screening.

The invention provides a deep eutectic solvent and a preparation method and application thereof, the effective components of the deep eutectic solvent are composed of choline chloride and ethylene glycol, the molar ratio of choline chloride to ethylene glycol is 1: 4, the water content of the effective components is 30%, and the deep eutectic solvent can replace an organic solvent to extract total flavonoids from fructus amomi; chemical components capable of distinguishing the varieties of the fructus amomi are determined through quantitative experiments on the fructus amomi flavonoid compounds, serve as differential markers epicatechin, procyanidine B2 and procyanidine B4 for distinguishing the varieties of the fructus amomi and have very high antioxidant activity, and H2O2-induced GES-1 cell oxidative stress is relieved by removing ROS in cells and up-regulating the level of SOD and GSH-Px.

The application discloses a preparation method of oat plant-based fermented milk rich in polyphenols based on a combined enzymolysis-fermentation technology. The preparation method comprises the following steps: after oat is soaked and cleaned, the oat is dried to obtain pretreated oat; the pretreated oat is baked and crushed, and then ball milled to obtain oat ultrafine powder; then, water is added to the oat ultrafine powder to obtain oat milk, the oat milk is stirred and subjected to high-speed shearing, the oat milk is completely dissolved and then gelatinized, and then enzyme is added to perform enzymolysis to obtain an enzymolysis solution; after the enzyme is inactivated, the enzymolysis solution is mixed and homogenized to obtain a homogenized solution, the homogenized solution is sterilized and cooled to obtain a uniform and stable system; finally, a strain is inoculated into the uniform and stable system to perform fermentation and post-ripening to obtain oat plant-based fermented milk. According to the preparation method, a nitrogen source does not need to be additionally added, the polyphenol content of the system and the acidity of the product can be effectively improved, and the fermentation time is shortened.

The invention provides a polypeptide beverage containing various amino acids and PQQ and a preparation method of the polypeptide beverage. It is found for the first time that PQQ combined with glutamine, asparagine and lysine can effectively improve the antioxidant activity of the beverage, the beverage has good DPPH free radical scavenging rate and superoxide anion scavenging rate, and a beverage product with nutrition and health functions can be prepared.

The application provides a synthesis method and application of silk fibroin-based melanin-like nanoparticles, and belongs to the technical field of nanoparticle preparations for biomedical use. After mixing silk fibroin nanoparticles, tyrosine and tyrosinase solution, silk fibroin-based melanin-like nanoparticles are obtained through one-pot copolymerization, and the enzyme concentration in the tyrosinase solution is 200-250 U / mL. The synthesis method has the advantages of simple operation, green environmental protection and the like, and the synthesized silk fibroin-based melanin-like nanoparticles have excellent antioxidant activity, ultraviolet protection and significant anti-skin cell photoaging.