46results about How to "Improves antioxidant activity" patented technology

The application belongs to the technical field of polymer materials, and particularly relates to a high-performance fiber composite material and a preparation method thereof. The preparation method of the high-performance fiber composite material comprises the following steps: adding pretreated aluminum oxide whiskers into a solvent, then adding pretreated carboxymethyl cellulose and gallic acid, and heating to reflux to obtain modified aluminum oxide whiskers; then mixing epoxy resin, the modified aluminum oxide whiskers, a curing agent and an accelerator uniformly to coat a film to obtain a resin adhesive film; and compounding the resin adhesive film and carbon fibers through hot pressing to obtain carbon fiber prepreg, and heating and curing the obtained prepreg to obtain the high-performance fiber composite material. The fiber composite material obtained by the application not only has high strength and rigidity, but also has excellent toughness, can effectively resist the impact of the external environment, has oxidation resistance and long-term stability, and has a wide application prospect in the fields of aerospace and new energy equipment.

The application discloses a preparation method of a hydrocolloid dressing for oral cavity, and relates to the technical field of medical supplies. Baicalin extract and a polyvinyl pyrrolidone solution are mixed to obtain a baicalin extract solution; chitosan fibers, hydroxymethyl cellulose and the baicalin extract solution are mixed to obtain a mixed solution; vacuum degassing: the prepared mixed solution is vacuumized, pressure is maintained, and primary degassing is performed; ultrasonic degassing: the mixed solution after primary degassing is subjected to ultrasonic degassing; static degassing: the solution after ultrasonic degassing is sealed by using a preservative film, and is subjected to secondary degassing at room temperature; a PU film is fixed in a film laying frame, the degassed mixed solution is poured into the film laying frame, and the film laying frame is placed into an air-blowing drying box to solidify and form a protective layer, so that the hydrocolloid dressing is obtained. The hydrocolloid dressing prepared by the method has good adhesion, can be used on a wound for a long time, and can effectively promote the repair of damaged cells and wounds, so that the healing of the wound in the oral cavity is promoted.

This invention discloses an optimized extraction and purification process for total flavonoids from Hypericum perforatum and its application in photodamage protection. Belonging to the field of natural product extraction technology, the process includes the following steps: using Hypericum perforatum powder as raw material, ultrasonic-assisted extraction is performed, followed by centrifugation, collection of the supernatant, and repeated extraction of the residue twice. The supernatants are combined to obtain a total flavonoid extract from Hypericum perforatum. The extract is then purified by adsorption using a macroporous adsorption resin, and the eluent is collected, concentrated, and dried to obtain high-purity total flavonoids from Hypericum perforatum. This invention features a stable process, high extraction efficiency, and is environmentally friendly, making it suitable for industrial production. The prepared total flavonoids from Hypericum perforatum exhibit high purity and significant antioxidant and photoprotective effects, and can be widely used in antioxidant, skin protection, and sunscreen products.

This invention belongs to the field of bio-fermentation technology, specifically relating to a high-glycemic-lowering active mulberry leaf fermentation broth, its preparation method, and its application. The preparation method includes the following steps: mulberry leaves are pulverized and sieved at low temperature; water and a carbon source are added to prepare a fermentation substrate; a bacterial suspension of *Lactobacillus mucilaginosus* and *Lactobacillus paracasei* is prepared; and the bacterial suspension is inoculated into the mulberry leaf fermentation substrate at a volume ratio for fermentation to obtain the high-glycemic-lowering active mulberry leaf fermentation broth. This invention selects *Lactobacillus mucilaginosus* CICC21800 and *Lactobacillus paracasei* CICC6263 in a certain proportion to form a fermentation microbial community. This community consists of two bacteria with different and complementary metabolic pathways, which can form a relatively stable biological community, mutually promoting each other. This community transforms macromolecules in mulberry leaves into smaller molecules and secretes beneficial polysaccharides, proteins, and other substances, increasing the content of effective active ingredients in the fermented product and exhibiting excellent fermentation effects on mulberry leaves.

This invention discloses a method for preparing resveratrol-loaded whey protein amyloid fibers based on moderate electric field modification. The method involves pretreating whey protein using a moderate electric field non-thermal effect, forming amyloid fibers through acid-thermal treatment, and further constructing a resveratrol delivery system based on protein fibrils. The resulting whey protein fiber-resveratrol complex exhibits excellent loading performance, antioxidant activity, and bioavailability, providing an effective carrier construction strategy for the efficient delivery and stabilization of hydrophobic and environmentally sensitive active molecules. This invention reveals the regulatory mechanism of the moderate-intensity electric field non-thermal effect in the protein fibrillation process, providing new technical ideas and theoretical references for the precise design of protein fiber structures and the development of functional protein-based delivery carriers, and providing technical support for the green preparation of protein fibrils and their in-depth development in functional foods.

The invention discloses low-caffeine anti-aging fermented tea and a preparation method thereof, and belongs to the technical field of tea processing. According to the invention, an Aspergillus Tennesensis strain is obtained by screening, and the preservation number of the Aspergillus Tennesensis strain is CGMCC (China General Microbiological Culture Collection Center) No.42415. The method comprises the following steps: inoculating aspergillus Tennesensis into a culture medium only containing tea leaves and water, fermenting, and airing after aspergillus Tennesensis hyphae completely cover the tea leaves, so as to obtain the low-caffeine anti-aging fermented tea. The aspergillus Tennesensis is used for degrading caffeine in the tea leaves, the growth speed is high, the degradation efficiency is high, and the degradation rate of the caffeine can reach 98% or above after 20 days of fermentation; the fermented tea leaves have very excellent antioxidant activity. The method for preparing the fermented tea is high in operability, the prepared fermented tea is red and dark in tea soup, black brown, mellow, smooth and low in bitterness degree, and the flavor quality of the tea leaves is improved.

The application provides a method for improving the flavor and quality of tilapia surimi products, and belongs to the technical field of aquatic product processing, and comprises the following steps: tilapia muscles are cut into small pieces, an improving agent is added, and the muscles are ground; the well-stirred surimi is made into fish cakes and shaped; the fish cakes are taken out after floating, and are placed in cold water for cooling; the shaped fish cakes are respectively fried at 150 DEG C, 180 DEG C and 210 DEG C for 4 min; the fish cakes are vacuum packaged and stored in a refrigerator after cooling; the flavor of the surimi is analyzed, the quality of the surimi is detected, and the deterioration of the surimi is determined and analyzed; threonine, creatinine and glucose are dissolved in a phosphate buffer to obtain A liquid; diethylene glycol is taken, mixed with soybean oil, and mixed with an improving agent by vortexing to obtain B liquid; the A liquid and the B liquid are fully mixed in a 48 mL sealed high-temperature pressure-resistant reaction bottle, and then are placed in an oil bath for reaction and then are placed in an ice water bath for reaction termination; the solution after reaction is removed and is reserved; the MeIQx content, precursor substances and intermediate substances of the MeIQx generation simulation system solution after reaction simulation are determined.

The application belongs to the technical field of medicine, and particularly relates to a composition for alleviating and repairing testicular damage caused by heat stress and application thereof. The composition for alleviating and repairing testicular damage caused by heat stress comprises the following raw materials: lycopene inclusion compound, ginsenoside Re, modified chitosan nanoparticles and a solvent. The amount of the modified chitosan nanoparticles is 10-15 wt% of the ginsenoside Re. The application innovatively designs a combination formula with lycopene and ginsenoside Re as the core. Both of the two main effective components in the formula are natural plant or traditional Chinese medicine extract, which effectively avoids the conflict between the medicinal properties of traditional Chinese medicines and has good biological safety. The composition can significantly repair the testicular damage caused by heat stress and accelerate the recovery after heat stress damage.

The application discloses a preparation method of oat plant-based fermented milk rich in polyphenols based on a combined enzymolysis-fermentation technology. The preparation method comprises the following steps: after oat is soaked and cleaned, the oat is dried to obtain pretreated oat; the pretreated oat is baked and crushed, and then ball milled to obtain oat ultrafine powder; then, water is added to the oat ultrafine powder to obtain oat milk, the oat milk is stirred and subjected to high-speed shearing, the oat milk is completely dissolved and then gelatinized, and then enzyme is added to perform enzymolysis to obtain an enzymolysis solution; after the enzyme is inactivated, the enzymolysis solution is mixed and homogenized to obtain a homogenized solution, the homogenized solution is sterilized and cooled to obtain a uniform and stable system; finally, a strain is inoculated into the uniform and stable system to perform fermentation and post-ripening to obtain oat plant-based fermented milk. According to the preparation method, a nitrogen source does not need to be additionally added, the polyphenol content of the system and the acidity of the product can be effectively improved, and the fermentation time is shortened.

The application provides a synthesis method and application of silk fibroin-based melanin-like nanoparticles, and belongs to the technical field of nanoparticle preparations for biomedical use. After mixing silk fibroin nanoparticles, tyrosine and tyrosinase solution, silk fibroin-based melanin-like nanoparticles are obtained through one-pot copolymerization, and the enzyme concentration in the tyrosinase solution is 200-250 U / mL. The synthesis method has the advantages of simple operation, green environmental protection and the like, and the synthesized silk fibroin-based melanin-like nanoparticles have excellent antioxidant activity, ultraviolet protection and significant anti-skin cell photoaging.

This invention discloses a fermentation process for a prickly pear and sea buckthorn compound fruit wine, belonging to the field of fruit wine fermentation technology. The invention includes the following steps: selecting prickly pear juice and sea buckthorn juice, mixing them in a 2:1 volume ratio as fermentation raw materials; adjusting the pH of the juice to a suitable range, adding sugar and preservatives, and adjusting the initial sugar content; activating yeast and adding it to the juice in a specific ratio, stirring evenly; placing the inoculated juice into a fermentation container and fermenting for 9 days under constant temperature conditions; filtering to remove sediment after fermentation to obtain primary fruit wine; clarifying the wine by low-temperature settling to promote impurity sedimentation; using moderate heating for sterilization to ensure product safety and stability; bottling after all indicators pass testing, and sealing and storing the finished product. This invention, by optimizing the compound ratio of prickly pear and sea buckthorn and the synergistic fermentation process parameters, can achieve an effective fusion and balance of the two raw materials in terms of flavor, nutrition, and fermentation kinetics.

The invention relates to a composition helpful for resisting oxidation and a preparation method thereof, and belongs to the technical field of functional foods. The composition helpful for antioxidation provided by the invention comprises yeast peptide, dunaliella salina extract, sparrow lychee extract and inula flower extract, the sparrow lychee extract is prepared by a fermentation process, and the four components cooperate to effectively help a human body to resist attack of free radicals and exert an antioxidation effect.

The application belongs to the field of biological medicine, and particularly relates to a sulfur-containing prodrug compound and medical use thereof. 1‑6 alkyl, substituted C 1‑6 alkyl, C 6‑10 aryl, substituted C 6‑10 aryl; R2 is selected from H or C 1‑6 alkyl, substituted C 1‑6 alkyl; R3 is selected from H or C1-6 alkyl, substituted C 1‑6 alkyl; R4 is selected from H, COOH or COOR 5 ; wherein R 5 is selected from C 1‑6 alkyl, substituted C 1‑6 alkyl or benzyl; L is selected from or a chemical bond; wherein X is selected from H, halogen, C 1‑6 alkyl, CH3O, CN or NO2; X is single substitution or multiple substitution at any position; Y is selected from O, S, NH or NCH3; R6 is selected from H or C 1‑3 alkyl; R7 is selected from H or C 1‑3 alkyl; n is selected from 1, 2, 3 or 4.

This invention relates to the food field and discloses a compound plant product, characterized in that the product comprises jujube extract, soybean peptide, corn oligopeptide, artichoke extract, amla, jujube seed, and optional additives; on a dry basis, the weight ratio of the jujube extract, soybean peptide, corn oligopeptide, artichoke extract, amla, and jujube seed is 1:(0.03-0.5):(0.03-0.5):(0.003-0.02):(0.005-0.05):(0.005-0.05); the tannin content in the product is 0-0.5 mg / g dry basis weight. The compound plant product provided by this invention, through the specific combination of the above components, exhibits higher antioxidant activity, better DPPH scavenging ability, and better ABTS scavenging ability; per gram dry basis, the tannin content is low, with minimal astringency and bitterness, resulting in a superior flavor.

This invention proposes a method for the biosynergistic extraction and refined utilization of all active ingredients in ginseng, belonging to the fields of medical, dental, or cosmetic formulations. This invention utilizes ginseng stress extract obtained through specific microbial treatment. This method leverages the stress stimulation of *Pseudomonas fluorescens* to efficiently synthesize various saponins, polysaccharides, and volatile oils in ginseng, resulting in an extract rich in components and with enhanced activity. Combining fractional extraction, resin purification, and nanoemulsification processes, a skincare raw material with comprehensive components, high stability, and excellent bioavailability is ultimately obtained, exhibiting a powerful synergistic effect in anti-inflammatory and anti-aging properties.

This invention belongs to the field of food technology, specifically relating to a fermented tomato cube and its preparation method. The preparation method includes the following steps: (1) Peel and seed fresh tomatoes, cut them into cubes, sterilize them, and place them in a sterile fermentation container. First, add *Lactobacillus casei*, mix evenly, and anaerobic ferment. Then, add *Lactobacillus rhamnosus*, mix evenly, and continue anaerobic fermentation. After sterilization, fermented tomato cubes are obtained; (2) Wash and peel onions, cut them into cubes, and pre-cook them to obtain pre-cooked onion cubes; (3) Mix the fermented tomato cubes, pre-cooked onion cubes, and seasonings evenly, cook at 80-85℃ for 20-25 minutes, and cool to room temperature to obtain fermented tomato cubes. The preparation method of this invention can ensure the efficient increase of total phenols in fermented tomato cubes while reducing the degradation of lycopene, achieving synergistic optimization of the nutritional quality of fermented tomato cubes, and improving the sensory quality of the final product.

This invention discloses a lutein composition for relieving eye fatigue and its preparation method, belonging to the field of functional foods. The process involves: reacting phycocyanin and kelp polyphenols to obtain modified phycocyanin; reacting fucoidan and β-glucuronidase to obtain modified fucose; reacting fish collagen peptides and zinc chloride to obtain collagen peptide-zinc chelate; reacting lutein silica cage complex and modified phycocyanin to obtain a first complex; reacting the first complex and modified fucose to obtain a second complex; reacting the second complex, collagen peptide-zinc chelate, and krill oil to obtain a lutein complex; reacting the second complex, collagen peptide-zinc chelate, and krill oil, then adding trehalose to obtain the lutein complex; and finally mixing the lutein complex with maltodextrin to obtain the lutein composition.

This invention discloses the application of an anionic open-ring cucurbituril derivative in improving the water solubility and antitumor activity of paclitaxel. The inclusion complex is formed by the assembly of anionic open-ring cucurbituril as the host molecule and paclitaxel as the guest molecule through non-covalent host-guest interactions, wherein the molar ratio of paclitaxel to anionic open-ring cucurbituril is 1-10:1. By forming a stable host-guest inclusion complex, the extremely poor water solubility and chiral stability of paclitaxel are significantly improved, effectively enhancing its antitumor efficacy and showing broad application prospects in targeted drug delivery and clinical oncology treatment.

The invention provides beta-glucan-quinoa protein interpenetrating double-network gel as well as a preparation method and application of the beta-glucan-quinoa protein interpenetrating double-network gel. The beta-glucan-chenopodium quinoa protein interpenetrating double-network gel is prepared from the following raw materials: chenopodium quinoa protein, beta-glucan and water, the concentration of the chenopodium quinoa protein in the gel is 5 to 25 percent, and the concentration of the beta-glucan in the gel is 3 to 5 percent. The beta-glucan is oat beta-glucan or barley beta-glucan, and the beta-glucan is beta-glucan or barley beta-glucan. The preparation method of the beta-glucan-chenopodium quinoa protein interpenetrating double-network gel comprises the following steps: uniformly mixing the chenopodium quinoa protein, the beta-glucan and water, heating in a water bath, carrying out gelation reaction, cooling after the reaction, and refrigerating and storing to obtain the beta-glucan-chenopodium quinoa protein interpenetrating double-network gel. The quinoa protein and the beta-glucan are used as raw materials, the adaptability is high, the enhanced double-network gel can be prepared under the neutral condition, and the stability is high. The gel provided by the invention has excellent water binding capacity and gel strength, and is relatively high in antioxidant activity.

The application belongs to the technical field of microcapsule preparation, and discloses a lutein microcapsule with high stability and antioxidant activity and a preparation method thereof. In the application, protein is dissolved, then Tagetes erecta polyphenol solution and lutein solution are added, and after sufficient stirring for a certain time, polysaccharide solution is added, pH is adjusted, and magnetic stirring is carried out for 1-2 hours, and then lutein microcapsule powder is obtained by freeze-drying. In the application, the lutein microcapsule is prepared by using Tagetes erecta polyphenol, protein and polysaccharide, so that the stability of lutein is improved, the antioxidant activity of lutein is enhanced, and the prepared Tagetes erecta polyphenol composite lutein microcapsule has high application value in the fields of food and medicine.

The present application relates to the technical field of functional microorganism screening and application, and particularly relates to a Lactobacillus plantarum with antioxidant and antibacterial functions and application thereof.The Lactobacillus plantarum is Lactobacillus plantarum WW strain, which has been preserved in the China General Microbiological Culture Collection Center on December 24, 2021, and the preservation number is CGMCC No.24189.The strain has strong acid and bile salt tolerance, outstanding antioxidant and antibacterial effects, and can be widely applied in the fields of food or medicine and the like.

This invention discloses a Bacillus subtilis ( Bacillus subtle The EB-8 strain, deposited on January 14, 2026, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37320, is disclosed. The application of Bacillus subtilis strain EB-8 in the fermentation modification of tartary buckwheat flour is also disclosed. This invention's Bacillus subtilis strain EB-8 can significantly increase the amylose content of tartary buckwheat, helping to inhibit starch retrogradation and improve its freeze-thaw stability; simultaneously, it increases the total flavonoid and total phenolic content of tartary buckwheat, enhancing its antioxidant activity. Furthermore, this strain can selectively retain and enrich essential amino acids such as lysine and methionine, reduce bitter components, improve taste, and facilitate the formation of a cereal matrix suitable for cold processing, with a mild taste and enhanced oxidative defense. Overall, it achieves synergistic optimization of the functional activity and processing characteristics of tartary buckwheat flour, which is beneficial for the high-value utilization of tartary buckwheat flour resources.

The invention relates to the technical field of Chinese wolfberry fruit fermented beverages, in particular to a probiotic fermented Chinese wolfberry fruit beverage as well as a preparation method and equipment thereof, the probiotic fermented Chinese wolfberry fruit beverage comprises the following raw materials in parts by weight: 15-25 parts of Chinese wolfberry fruits, 3-5 parts of food-grade marigold, 5-10 parts of black tea, 15-25 parts of mulberries, 10-20 parts of blueberries, 1-3 parts of red dates and 1-3 parts of fresh ginger. The preparation method comprises the following steps: pre-fermentation of black tea, main fermentation after addition of the wolfberry and chrysanthemum juice, and post-fermentation after addition of mulberries and blueberries. Through scientific raw material proportioning, multi-stage fermentation and fine control, the health-care beverage has the advantages of more comprehensive nutrition and functional components, better health-care effect, better taste and higher product stability.

The present application relates to a kind of soybean polypeptide preparation method with antioxidant and antagonistic lead damage effect.The soybean polypeptide prepared in the present application has good DPPH free radical clearance rate, ABTS free radical clearance rate and Fe3+ reduction capacity in vitro;It can also alleviate the case that the body weight of mouse is slowly increased due to lead exposure, improve the learning and memory ability of lead exposure mouse, alleviate the organ damage of mouse caused by lead exposure, enhance the level of reduced glutathione, superoxide dismutase and catalase of organism, and alleviate the abnormal increase of malondialdehyde caused by lead exposure.The soybean polypeptide prepared in the present application has good in vitro antioxidant capacity, and has antagonistic effect on oxidative stress caused by lead exposure mouse, and can be used as non-toxic and harmless natural antioxidant.For the development of natural antioxidant food, it has far-reaching application prospect, and also provides a new feasible idea for the combined application of bioinformatics technology and traditional enzymatic technology.

The present application belongs to the field of microbial application and feed processing technology, and discloses a bacillus subtilis, named bacillus subtilis YT1, which is preserved in China Center for Type Culture Collection, located at Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, with a preservation number of CCTCC NO: M 20242767 and a preservation date of December 10, 2024. The present application also discloses a microbial preparation and the application of the bacillus subtilis or the microbial preparation in the processing of silage. The present application first simultaneously possesses high efficient lignocellulose degradation ability, excellent fermentation promotion characteristics, significant antioxidant activity and good probiotic characteristics in a strain of bacillus subtilis. This characteristic enables a single bacterial agent to achieve multiple goals such as fiber degradation, fermentation promotion, oxidation protection, which traditionally requires multiple compounded bacterial agents, and fundamentally overcomes the problems of poor compatibility between strains, unstable synergistic effect and high application cost of existing compounded additives.

This invention discloses a novel use of Artemisia annua seeds, a medicinal and edible compound composition for relieving hangovers and protecting the liver, chewable tablets, and their preparation process. This invention is the first to discover that the mucilage in Artemisia annua seeds slows down ethanol diffusion through a dual mechanism of physical adsorption and three-dimensional network blockage, thus slowing down the rate of ethanol absorption in the digestive tract, reducing the peak blood plasma concentration, and delaying or prolonging the time of intoxication. Therefore, it can be used in the preparation of health products / functional foods that protect the stomach, prevent intoxication, and delay alcohol absorption. Based on this discovery, this invention provides a medicinal and edible compound composition for relieving hangovers and protecting the liver, composed of 10-15 parts by weight of Pueraria lobata, 10-15 parts by weight of Hovenia dulcis, 8-10 parts by weight of Lilium brownii, 4-8 parts by weight of Poria cocos, 4-8 parts by weight of Dioscorea opposita, and 4-8 parts by weight of Artemisia annua seeds. Furthermore, to balance rapid effectiveness and compliance, it is formulated as a chewable tablet. This chewable tablet can significantly prolong the time of intoxication and shorten the sobering-up time, and is significantly superior to the group lacking Artemisia annua seeds in terms of antioxidant indicators (SOD, GSH, MDA) and hangover relief effect.

The invention belongs to the technical field of biology, and provides pediococcus acidilactici H-3 for producing tannase and application of the pediococcus acidilactici H-3. The invention discloses pediococcus acidilactici H-3, the Latin of the pediococcus acidilactici H-3 is pediococcus acidilactici, the preservation name of the pediococcus acidilactici H-3 is pediococcus acidilactici, the preservation place is China General Microbiological Culture Collection Center, the preservation date is January 13, 2026, and the preservation number is CGMCC No.37372. The invention further discloses a preparation method of the pediococcus acidilactici H-3. According to the invention, an excellent strain resource is provided for improving the flavor of fruits with high tannin content and developing the functional characteristics of the fruits, and a new direction is provided for the application of the strains in plant matrix fermentation.

This invention discloses a method for preparing Yuanjiang asparagus extract and its application. The method uses fresh Yuanjiang asparagus tender stems as raw material. After selection, washing, freeze-drying, pulverizing, and sieving, choline chloride and 1,2-propanediol eutectic solvent are added for constant-temperature extraction. After ultrasonic temperature control, cellulase and pectinase are added for complex enzymatic hydrolysis. After heating to inactivate the enzymes, centrifugation is performed, and the supernatant is collected to obtain the crude extract of Yuanjiang asparagus saponins. This method has high extraction efficiency, and the obtained asparagus saponins have strong antioxidant activity, exhibiting strong scavenging ability against DPPH, ABTS, and hydroxyl radicals, as well as Fe... 2+ The chelation rate was high, and it showed significant in vitro inhibitory effects on the proliferation of human colorectal cancer cells Caco-2 and human hepatocellular carcinoma cells HepG2. The raw material used in this method is fresh, tender stems of Yuanjiang asparagus, which is widely available and of excellent quality. The extraction process is green, environmentally friendly, and simple, not only improving the comprehensive utilization value of Yuanjiang asparagus but also providing a new method for extracting natural saponin active ingredients. The obtained asparagus saponins can be used to develop antioxidant and anti-tumor functional foods, showing potential market prospects.

This invention provides a method for extracting chlorogenic acid, a coffee byproduct. Using fresh coffee peel and coffee grounds as raw materials, chlorogenic acid is extracted using ethanol extraction and ultrasound-assisted extraction. Through single-factor and orthogonal experiments, the average chlorogenic acid content in the extract from fresh coffee peel under the conditions of 50% ethanol concentration, 60 min of ultrasound, 40℃ ultrasound temperature, and a solid-liquid ratio of 1:12 (g / mL) was 0.075 mg / mL. The average chlorogenic acid content in the extract from coffee grounds under the conditions of 50% ethanol concentration, 6 h of extraction time, and a solid-liquid ratio of 1:15 (g / mL) was 0.059 mg / mL. Antioxidant activity assays showed that the chlorogenic acid extracted from both coffee byproducts exhibited good antioxidant activity. This invention not only reduces the waste of coffee byproducts but also realizes their deep processing and high-value utilization, which is conducive to promoting the sustainable development of the coffee industry.

This invention discloses an alkaline protease mutant and its application in the preparation of active hippocampal peptides. This alkaline protease mutant exhibits high enzyme activity and high thermal stability, with an optimal operating temperature of 60°C. Even after treatment at 70°C for 90 minutes, it retains 23.45% of its relative enzyme activity, demonstrating excellent thermal stability. When used for the enzymatic hydrolysis of defatted hippocampal powder, it can increase the yield of active hippocampal peptides from 11.7% to 82.4%, and improve the antioxidant activity (IC50) of the hydrolysate. 50 The enzyme activity was increased by approximately 51.4%, effectively solving the bottleneck of the difficulty in synergistically improving enzyme activity and thermal stability in existing technologies. This provides an excellent catalyst and process solution for the industrial-scale, efficient, and low-cost production of hippocampal active peptides.