Extraction method of anthocyanin and original anthocyanin components in cranberry fruits

An extraction method and proanthocyanidin technology are applied in the directions of medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc., which can solve the problems of high preparation cost, complex process and high energy consumption, and achieve low cost , the process is simple, the effect of high yield

Inactive Publication Date: 2011-04-06
宝鸡市虹源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing method for extracting anthocyanins and proanthocyanidins from cranberry fruit (Chinese Patent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1, get 1kg of fresh cranberry fruit, pulverize it into particles with a diameter of 0.5~0.6cm, add deionized water of 1 times the weight of the fresh fruit, add cellulase to be 0.5% of the weight of the fresh fruit, 45~50 ℃ of insulation and stirring for 12 hours, Add 3 times the weight of fresh fruit water, adjust the pH value to 2.0 with edible hydrochloric acid, filter after stirring and leaching for 1 hour, extract with water once again in the same way, and combine the water extracts; add 7 times the weight of the residue to the residue. The concentration is 70 % ethanol solution, solid-liquid separation after reflux extraction, the extract is decompressed and ethanol is recovered to obtain alcohol extract concentrate; the water extract and alcohol extract concentrate are combined and filtered, the filtrate is passed through macroporous adsorption resin D101, and deionized water is used. Wash until the color of the effluent is light, and then use 10-80% conce...

Example Embodiment

[0032] Example 2, get 5kg of fresh cranberry fruit, pulverize it into particles with a diameter of 0.5~0.6cm, add deionized water of 1 times the weight of the fresh fruit, add pectinase to be 1.0% of the weight of the fresh fruit, adjust the pH value to 5.0 with citric acid, Keep stirring at 40-50°C for 6 hours, add 5 times the weight of fresh fruit water, adjust the pH to 3.0 with citric acid, stir and extract for 2 hours, filter, extract with water once again in the same way, and combine the water extracts; Add 6 times the weight of the residue to the ethanol solution with a concentration of 50%, and after reflux extraction, solid-liquid separation is performed, and the extract is subjected to reduced pressure to recover ethanol to obtain an alcohol-extracted concentrate. After combining the water extract and the alcohol-extracted concentrate, the filtrate passes through The macroporous adsorption resin D201 was washed with deionized water until the color of the effluent was ...

Example Embodiment

[0033]Example 3: Get 5kg of fresh cranberry fruit, pulverize it into granules with a diameter of 0.5 to 0.6 cm, add deionized water of 1 times the weight of the fresh fruit, add amylase to be 2.0% of the weight of the fresh fruit, adjust the pH to 5.5, 45 with edible phosphoric acid Keep stirring at ~50°C for 2 hours, add 8 times the weight of fresh fruit water, adjust the pH value to 1.0 with edible phosphoric acid, stir and extract for 2 hours, filter, extract twice with water in the same way, and combine the water extracts; add to the residue Methanol with a concentration of 60% by 5 times the weight of the residue, solid-liquid separation after reflux extraction, recovery of methanol from the extract under reduced pressure to obtain an alcohol-extracted concentrate, the above-mentioned water extract and alcohol-extracted concentrate are combined and filtered, and the filtrate passes through the macropores The adsorption resin AB-8 was washed with deionized water until the c...

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PUM

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Abstract

The invention discloses an extraction method of anthocyanin and original anthocyanin components in cranberry fruits, which comprises the following steps: crushing the cranberry fruits, enzymolyzing the crushed fruits with biological enzyme, and lixiviating with water under the condition of the pH value being in the range of 1.0-5.5 to obtain water extracting solution and residues; carrying out alcohol extraction on the residues, concentrating the obtained extracting solution and recovering alcohol to obtain alcohol extraction concentrated solution; and merging the water extracting solution with the alcohol extraction concentrated solution, adsorbing with macroporous absorption resin, washing the macroporous absorption resin with water, analyzing with water-containing alcoholic solution, and concentrating and drying the obtained analytical liquid to obtain a mixture containing the anthocyanin and original anthocyanin components. The extraction method of the invention has the advantages of simple process, strong equipment generality, small investment, high yield, low cost and applicability to large-scale production; and the obtained mixture can be used as a raw material of foods, healthcare products and medicines or an additive.

Description

technical field [0001] The invention relates to a method for extracting anthocyanin and proanthocyanidin components in cranberry fruit, and the extract can be used as a raw material for food, skin care products and medicines or as an additive. technical background [0002] Cranberry (Vaccinium macrocarpaon Aiton) is a plant of the Rhododendronaceae Vaccinium genus, also known as cranberry, commonly known as cranberry in Taiwan; it is distributed in North America, Canada and the United States, and the growth environment is a wet and humid place, adapting to pH4~4.5 The acidic soil, the fruit is red, 1-1.5cm in diameter, blooms from June to August, and matures from August to October. As early as in the United States, massa plunger began to be cultivated. At present, the cultivation area in the United States is relatively large, and it has been introduced to Russia and other European regions in recent years. The world's cultivated area is 370 million acres. [0003] Cranberry...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L1/30A23L1/275A61K36/45A61K8/97A61P39/06A61P29/00A61P35/00A61P9/00A61Q1/02A61K131/00A23L5/43A23L33/105
Inventor 范建礼魏有良
Owner 宝鸡市虹源生物科技有限公司
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