101 results about "Vaccinium" patented technology

The invention provides a preparation process of blueberry concentrated juice with high content of anthocyanin. According to the preparation process provided by the invention, pectase is adopted for performing enzymolysis on blueberry pulp, chitosan is adopted as a clarifying agent for obtaining clear blueberry juice, a decompression concentration process is adopted for preparing the blueberry concentrated juice, and microwave sterilization is adopted for sterilizing the blueberry concentrated juice. The preparation process comprises the following steps of: selecting blueberry fruits in vaccinium ashei gardenblue species; extracting the blueberry juice, and adding the pectase to perform the enzymolysis on the blueberry pulp; clarifying the blueberry juice, and adding the chitosan as the clarifying agent for obtaining the clear blueberry juice; concentrating the blueberry juice, and adopting the decompression concentration to obtain the blueberry concentrated juice; and performing sterile filling on the blueberry juice, and adopting the microwave sterilization to sterilize the blueberry concentrated juice, wherein the content of soluble solids in the blueberry concentrated juice is 60%-75%, and the content of the anthocyanin is 1.925g/L-3.384g/L. The preparation process provided by the invention can not only effectively prevent the loss of the anthocyanin in the blueberry concentrated juice, but also shorten the concentration time, control the production cost of the preparation process and effectively protect color, luster, fragrance and other sensory qualities of the blueberry juice.

The invention discloses a construction method for a vaccinium ashei tissue culture rapid propagation system and relates to a plant tissue culture method. According to the invention, a stem of a water culture seedling is selected as an explant, after being treated by ethyl alcohol and mercury bichloride, the stem is inoculated to a stem shoot system induction culture medium and then is vertically inserted into stem shoot system multiplication culture, in the rooting culture, the concentration of IBA is 2.0 mg L<-1>, and the rooting percentage is greater than 85%. According to the invention, the water culture seedling is used as the source, and the contamination rate and the browning rate are obviously lower than that of the greenhouse culture seedling; the ethyl alcohol disinfection and the mercury bichloride are used for disinfection to mutually promote each other; the stem shoot system induction and the multiplication are consistent with an orthogonal experiment result; the influence of IBA on rooting reflects the result of a single-factor randomized block experiment.

The invention discloses oyster polysaccharide fruit juice which takes oyster polysaccharide as the major component and giant salamander oligosaccharide peptide, black currant, vaccinium vitisidaea, citric acid and pectin as accessories and has the effects of reducing blood pressure, protecting liver, improving sleep and resisting radiation damage. The oyster polysaccharide fruit juice comprises the following active ingredients in percentage by mass: 0.1-10% of oyster polysaccharide, 0.1-10% of giant salamander oligosaccharide peptide, 0.1-10% of lyophilized powder of black currant, 0.1-10% oflyophilized powder of vaccinium vitisidaea, 0.01-0.6% of citric acid, 0.1-2% of pectin and the balance of water.

A non-food anti-microbial-adhesion and aggregation composition comprising a suitable carrier and an effective amount of an adhesion inhibitory fraction isolated from juice from berries of the Vaccinium plant genus. In an embodiment the anti-aggregation and adhesion fraction is isolated from cranberry juice. It is characterized as being polymeric and having a molecular weight ≧14,000; an elemental analysis of carbon 43-51%, hydrogen 4-5%, no nitrogen, no sulfur and no chlorine; a nuclear magnetic resonance (NMR) line spectrum as set forth in FIGS. 2A and 2B; and an ultraviolet spectrum with an absorption peak at 280 nm in neutral or acidic pH solution which is absent in alkali solutions. This fraction exhibits adhesion inhibitory activity against P fimbriated bacteria, oral bacteria and Helicobacter pylori.

The invention belongs to the technical field of plant vegetative propagation, and particularly relates to a method for vegetative propagation of vaccinium plants through fresh sphagnum. The method is particularly applicable to rapid breeding of some main vaccinium plants. Half-lignified branches or tissue culture seeding stems of bilberry in that very year are selected and soaked in an indolebutyric acid, and then cuttings are arranged at the roots for 2 hours. A fresh sphagnum culture substrate is inoculated with endotrophic mycorrhiza extracted from the vaccinium plants. The cuttings are inserted in the fresh sphagnum culture substrate, and then plastic film mulching is conducted. After cuttage, the temperature is controlled to range from 15 DEGC to 28 DEG C, and relative humidity is controlled to range from 80% to 90%; after the cuttings take roots, liquid complete fertilizer with the concentration of about 0.5% is applied every 20 days. According to the method, the fresh sphagnum is used as the substrate for culture of seedlings of the vaccinium plants in a cuttage mode, the survival rate of the cultured seedlings of the vaccinium plants is increased by means of inoculating the substrate with the mycorrhiza of the vaccinium plants, and therefore it is guaranteed that strong and high-quality seedlings without pest and disease damage are cultured.

The invention relates to a health-care bonsai of a blueberry and vaccinium combined plant cultivated through wind-solar hybrid type power supply and energy-saving illumination, and belongs to the technical field of illumination application of new energy sources of plant bonsais. A vaccinium root grows in potted soil matrix of a health-care plant planting pot, and grows with a vaccinium stem, the upper end of the vaccinium stem and the lower section of a blueberry stem are slantwise cut by a grafting knife, the grafted part is applied with a growth promoting adhesive sleeve to realize the early grafting survival of the vaccinium and the blueberry, and the blueberry stem grows with leaves and fruits. A photovoltaic illumination cutting rod which utilizes solar power generation is inserted into the potted soil matrix of the sun facing surface of the bonsai, and a wind power illumination cutting rod which utilizes the wind power generation is inserted into the potted soil matrix of the shady side of the bonsai. As the illuminations of the middle part and the lower part of the bonsai plant are increased by the power illumination which is provided by clean energy sources such as solar energy and wind energy, and the electric energy is inputted and stored into a lithium ion battery, the continuous power supply and illumination can be provided at night or in rainy days, the bonsai plant can grow well, the color of the blueberry fruit is changed from green to blue, and the fruit is fragrant.

The invention relates to a method for extracting pigments from the leaves of black rice leaves, comprising the following steps: (1) crushing the dried black rice leaves, adding 2-5 times the amount of 5% acetic acid solution to soak for 2-4 hours, and extracting 2-4 times , filter with a 80-160 mesh filter to obtain the filtrate; (2) The filtrate is subjected to high-speed centrifugation at 2500-2900rpm to obtain the supernatant; (3) The supernatant is added to the membrane separation system for ultrafiltration and nanofiltration to obtain The concentrated solution is dispersed with water, adsorbed on a polyamide column, washed with water to remove impurities, and then eluted with 5-7BV50% ethanol solution, concentrated eluent, and freeze-dried to obtain the leaf pigment of U. The leaf pigment extracted by the invention has high stability and high purity, less energy consumption and less pollution in the production process, and is suitable for industrialized production.

The present invention discloses a vacinium bracteatum leaf freeze-dried powder, its preparation method and application. Said preparation method includes the following steps: collecting vaccinium bracteatum leaves, sorting, cleaning, breaking, pressing to obtain vaccinium bracteatum leaf juice, filtering, removing impurity, making centrifugal purification, concentrating, vacuum freeze-drying to obtain dried solid, pulverizing to obtain the invented vaccinium bracteatum leaf freeze-dried powder. It can be directly made into various health-care foods of vaccinium bracteatum wine and vaccinium bracteatum beverage, etc.

The invention discloses a vaccinium australe tissue culture and rapid propagation method. Vaccinium australe has the advantages of strong stress resistance, high growth speed and high yield. Therefore, establishment of a vaccinium australe in-vitro regeneration system is very necessary so as to lay a foundation for the cultivation of vaccinium australe with high quality, high yield and strong adaptability. According to the vaccinium australe tissue culture and rapid propagation method disclosed by the invention, stems with buds of vaccinium australe are taken as explants, a vaccinium australe in-vitro regeneration plant can be prepared by virtue of the steps such as induction culture, multiplication culture, rooting culture as well as acclimatization and transplanting, and thus the tissue culture and rapid propagation of vaccinium australe can be achieved so as to ensure that a new fast and effective way can be provided for mass propagation of vaccinium australe.

The invention relates to a method for improving rooting of vaccinium ashei tissue culture seedlings. The method is characterized in that high-quality vaccinium ashei adventitious buds obtained by primary culture and subculture proliferation are used as materials, vermiculites are used as substrates, and the materials are induced on a 1/2 WPM culture medium added with suitable-concentration NAA to take roots, wherein the materials are induced and cultured for 40-60 days and the rooting rate can reach more than 95%; and the rooting quantity of a single plant is more than 20, the length of each root is 2.0cm-3.0cm and rooted seedlings grow healthy and strong, the transplanting survival rate is high and the recovery period is short. The rooting substrates which save cost and have high efficiency are selected and conventional tissue culture conditions are adopted, so that the rooting rate of the vaccinium ashei tissue culture seedlings is improved by 34.7% and the transplanting survival rate is improved by 60.3%; the problems in current production that the rooting rate of the vaccinium ashei tissue culture seedlings is low, the transplanting is difficult and the like are solved; and a high-quality efficient propagation method is provided for producing high-quality variety germchits of vaccinium ashei and reference is provided for other types of blueberry tissue culture seedlings.

The present invention concerns the use as anti-viral agents of isolated fractions from berry juice of the plant genus Vaccinium. Two specific fractions are provided: (a) polymeric material having a molecular weight ≧12,000; and (b) proanthocyanidins-containing fraction. Thus, the invention provides methods of treating a subject against a viral infection; the method comprises providing said subject with at least one of the above isolated fractions in an amount effective to prevent production of a viral infection in said subject. A specific and preferred viral infection according to the invention is an influenza virus infection.

The invention firstly discloses a method for extracting the leaf pigment of black rice tree. The extraction method comprises the steps of producing a pigment precursor by using the leaves of the black rice plant as a raw material, and then producing the pigment through processes such as enzyme-catalyzed oxidation, polyamide column purification, spray drying and the like. Compared with the existing technology, the present invention has the following advantages: 1, it dries and breaks the leaves of black rice leaves, which increases the reaction area of ​​the leaves of black rice leaves, and makes the extraction of pigment more fully; 2, utilizes organic solvents to carry out Extraction, large amount of pigment dissolution, high extraction efficiency; 3. Select a higher concentration of ethanol aqueous solution or acetone aqueous solution as the extraction solvent, which can precipitate the dissolved macromolecular protein, thereby filtering and removing it, and the filtering effect is more significant; 4. Using Membrane filtration is a physical impurity removal, which not only has low energy consumption and low pollution, but also can reduce the decomposition of pigment due to high temperature and improve the recovery rate of pigment.

The invention relates to bacillus and a method for applying the bacillus to producing isovaleric acid. The bacillus is separated from a tobacco leaf on an alcoholization stage through a selective culture medium, and is already preserved in the general microbiological center of the China microbe preservation management committee on September 13, 2017, and the preservation number is CGMCC No.14609.The strain takes the waste of the tobacco industry as the raw material to synthesize the isovaleric acid, and the isovaleric acid is a colorless sticky liquid, and has a sweet fruit aroma and the fragrance of the vaccinium uliginosuma after being highly diluted. A fermentation culture medium used in the process of synthesizing the isovaleric acid through the bacillus is low in cost, a large quantity of wastes produced in the tobacco industry are also treated, the method plays positive roles in protecting the environment and reasonably utilizing the resources, and has an excellent application prospect.

The invention discloses a method for cutting and seedling raising of vaccinium bracteaturn. Softwood cutting is adopted, wherein branch spikes of wild vaccinium bracteaturn which transplanted for 2 years and grows robustly are cut, and the branch spikes are semi-lignified and similar in maturity and have no diseases and pests; hardwood cutting is adopted, wherein vaccinium bracteaturn branches which grow robustly for 2 years and have no diseases and pests are cut; hardwood cutting is conducted in a cutting seedling bed of a greenhouse, and a small plastic arched shed is set up; softwood cutting is conducted on a full-illumination automatic intermittent spraying seedling bed, and cutting mediums are perlite, peat soil, vermiculite and river sand. Before cutting is conducted, 0.05 potassium permanganate is adopted for disinfection treatment. Rooting agents are IBA, ABT1 rooting powder and NAA. The method for cutting and seedling raising of vaccinium bracteaturn has the advantages that the problem is solved that propagation offspring of vaccinium bracteaturn seeds have big differences, excellent characteristics of parent varieties can be well kept, the operation is simple, the cost is low, the propagation speed is high, and the survival rate is high; a large number of the vaccinium bracteaturn seeds can be quickly obtained, and the method has good popularization value.

The invention discloses a proliferation culture medium for tissue culture of Jietu vaccinium vitisidaea. The proliferation culture medium comprises an improved WPM (woody plant medium), 2mg/L zeatin, 30g/L sucrose and 8g/L agar; the pH value is 4.8-5.0; each liter of improved WPM comprises a large quantity of elements including 1071.6mg of potassium nitrate, 370mg of magnesium sulfate, 96mg of calcium chloride and 170mg of potassium dihydrogen phosphate, ferric salts including 37.3mg of ethylene diamine tetraacetic acid and 27.8mg of ferrous sulfate, microelements including 22.3mg of manganese sulfate, 8.6mg of zinc sulfate, 0.025mg of copper sulfate, 0.25mg of sodium molybdate and 6.2mg of boric acid, and organic matters including 0.5mg of nicotinic acid, 0.5mg of thiamine hydrochloride, 0.5mg of pyridoxine hydrochloride, 100mg of inositol and 2mg of glycine. According to the culture medium, the proliferation rate reaches 9.8 after 30 days.

The invention provides a detection method for rapidly and accurately identifying ericaceae vaccinium. Following primer pairs are used for detection. Nucleotide sequences of the primer pairs are shownas follows: the nucleotide sequences of VcSSR11-F and VcSSR11-R are shown in SEQ ID NO:1 and SEQ ID NO:2; the nucleotide sequences of VcSSR14-F and VcSSR14-R are shown in SEQ ID NO:3 and SEQ ID NO:4;the nucleotide sequences of VcSSR19-F and VcSSR19-R are shown in SEQ ID NO:5 and SEQ ID NO:6; the nucleotide sequences of VcSSR28-F and VcSSR28-R are shown in SEQ ID NO:7 and SEQ ID NO:8; the nucleotide sequences of VcSSR33-F and VcSSR33-R are shown in SEQ ID NO:9 and SEQ ID NO:10. In addition, the invention provides an establishing method of allele image patterns of the ericaceae vaccinium. By reference to the method, the allele image patterns are expanded, and the number of the available allele image patterns is increased.

The invention provides purple ginseng prepared by a vaccinium fermentation method and application thereof in medicine. The purple ginseng prepared by adopting fermentation of vaccinium fermentation broth contains rare saponin component of ginseng and anthocyanidin substances, and can be used for preparing medicaments for treating gastric cancer, lung cancer and liver cancer, treating myocardial injury caused by antibiotic medicaments, and improving the body hypoxia tolerance. The fermentation process is convenient and simple for operation, and lactobacillus bulgaricus is added in the fermenting process, so that the time for preparing the fermentation broth is greatly shortened, the fermentation efficiency is improved, and the content of rare saponin Rg5 is higher. Therefore, the purple ginseng is suitable for large-scale industrial production.

The invention relates to a grafting breeding method for improving the adaptability of the large-fruit type Vaccinium blueberry variety in a subtropical zone by taking blueberries as stocks. The grafting breeding method is characterized in that Croaton is taken as the stocks in the subtropical zone cultivation of the large-fruit type Vaccinium blueberry variety Spartan to carry out grafting improvement so as to improve the growth and development of the Vaccinium blueberry variety, and improve the adaptability, the fruit yield and the quality of the Vaccinium blueberry variety. According to the grafting breeding method, a plant grafting technology is applied to the breeding and the cultivation of blueberry nursery stocks, a proper grafting mode and a stock type of the Vaccinium blueberry variety Spartan are tested, grafted seedlings are subjected to cultivation observation for few years, the proper grafting mode and a stock-scion combination are established, the ecological adaptability and the fruit yield and quality of the Spartan blueberry in the Yangtze River delta zone in China are improved, and the grafting breeding method has an outstanding meanings in the blueberry grafting breeding research and application which is rare worldwide.

The invention relates to Vaccinium fruit extracts, related preparations, production methods and uses.

The present invention relates to an extract derived from Vaccinium and to the process for obtaining this extract. This proanthocyanidin-rich extract can be used as a dietary or nutraceutical preparation.

The present invention provides a visual fatigue alleviating pharmaceutical combination preparation and a preparation method thereof, wherein the visual fatigue alleviating pharmaceutical combination preparation comprises a capsule skin and a capsule core, and is characterized in that the capsule core is prepared from the following raw materials by weight: 80-200 parts of a vaccinium extract, 20-100 parts of lutein, 5-30 parts of beta-carotene, 200-400 parts of vegetable oil, and 5-20 parts of beeswax. The visual fatigue alleviating pharmaceutical combination preparation of the present invention has advantages of refined prescription, high effective component content and effective improvement of clinical symptoms such as eye pain, eye swelling, photophobia, blurred vision, dry eye and the like, and provides effects superior to the composition in the prior art.

The invention provides a method for preparing dried blueberry fruit powder. After washing, crushing, mixing blueberry puree and maltodextrin solution in proportion, homogenizing, pre-freezing, freeze-drying and other steps, the dried blueberry fruit powder is prepared as a semi-finished product The semi-finished product of dried blueberry fruit powder is quickly ground and sieved to obtain the dried blueberry fruit powder product, which is sealed and preserved. Southern highbush blueberry dried fruit powder can be widely used in food and health products, especially in the preparation of different varieties of blueberry dried fruit powder, and can also be extended to the preparation of more varieties of dried berry powder. The invention uses the maltodextrin carrier and adopts the freeze-drying method, the dry powder has a crisper structure, and the obtained dry powder is finer and more suitable for commercialization. Compared with the dry powder without carrier, it has the characteristics of less viscous sugar liquid on the surface, slower moisture absorption speed, and lower moisture return speed.