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Construction method for vaccinium ashei tissue culture rapid propagation system

A technology of rabbit-eye blueberry and construction method, which is applied in the field of plant cultivation and can solve problems such as the impact of disinfection effect

Inactive Publication Date: 2014-02-19
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, during the establishment of blueberry sterile culture lines, there were few reports on the influence of the source of materials on the disinfection effect

Method used

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  • Construction method for vaccinium ashei tissue culture rapid propagation system
  • Construction method for vaccinium ashei tissue culture rapid propagation system
  • Construction method for vaccinium ashei tissue culture rapid propagation system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] (1) with L 18 (2×3 7 ) to investigate the effects of alcohol and mercury chloride on the disinfection of the young stems of the hydroponic rabbit-eye blueberry

[0028] The medium combination is 1 / 2MS+5g / L Agar+30g / L Sucrose, adjust the pH to 5.8, dispense into 175mL glass bottles with plastic caps from Sigma Chemical Co., and sterilize at 121°C for 20min. The explants were the young stems of three kinds of rabbit-eye blueberries grown in hydroponics and in the field of ‘Brightwell’, ‘Premier’ and ‘Garden Blue’, collected from the experimental field of Southwest Forestry University. Soak the leaves with 0.1% detergent for 1min, then rinse them with running water for 2h, put them on the ultra-clean bench, use L 18 (2×3 7 ) Orthogonal experiment to study the effect of 70% alcohol and 0.1% mercuric chloride on the disinfection of explants, followed by soaking in sterile water for 3 times, 5 minutes each time. Insert the single-bud stem segments on the medium. The expe...

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Abstract

The invention discloses a construction method for a vaccinium ashei tissue culture rapid propagation system and relates to a plant tissue culture method. According to the invention, a stem of a water culture seedling is selected as an explant, after being treated by ethyl alcohol and mercury bichloride, the stem is inoculated to a stem shoot system induction culture medium and then is vertically inserted into stem shoot system multiplication culture, in the rooting culture, the concentration of IBA is 2.0 mg L<-1>, and the rooting percentage is greater than 85%. According to the invention, the water culture seedling is used as the source, and the contamination rate and the browning rate are obviously lower than that of the greenhouse culture seedling; the ethyl alcohol disinfection and the mercury bichloride are used for disinfection to mutually promote each other; the stem shoot system induction and the multiplication are consistent with an orthogonal experiment result; the influence of IBA on rooting reflects the result of a single-factor randomized block experiment.

Description

technical field [0001] The invention belongs to a plant cultivation method, in particular to a plant tissue culture method. Background technique [0002] Blueberry is an important economic forest tree with dark blue fruit, delicate flesh and unique flavor. It is known as the "Queen of Fruits" and "Fruit of Color Contact". It is listed as one of the five health foods by the International Food and Agriculture Organization. Blueberry has certain nutritional value and medicinal value, and the pulp is rich in nutrients such as vitamins, proteins and minerals (Debnath & McRAE, 2001; Debnath, 2005). Rabbiteye Blueberry ( Vaccinium ashei. ) is a perennial deciduous shrub of the genus Vaccinium in the family Rhododendronaceae (Vander, 1988; Ratnaparkhe, 2007). Lohachoompol (2008) believed that the anthocyanin content of rabbit eye blueberry was significantly higher than that of highbush blueberry, and the rich anthocyanin pigment could relieve eye fatigue and improve human vision....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张太奎刘小珍张汉尧
Owner SOUTHWEST FORESTRY UNIVERSITY
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