299 results about "Shoot" patented technology

The invention belongs to the technical field of cosmetic preparation, and particularly relates to a skin moisturizing and repairing composition based on dendrobium officinale and fibroin as well as a preparation method and application of the skin moisturizing and repairing composition. Essence extracted from dendrobium officinale tender shoots obtained through a plant stem cell culture technology and stepped molecular weight fibroin prepared through a composite enzymolysis technology serve as raw materials, the safe and efficient composition is developed, and the actual requirements for skin moisturizing and repairing are met.

The present disclosure provides methods of gene transformation of sunflower based on Agrobacterium-mediated gene delivery to meristem cells of fresh imbibed / germinated sunflower seeds. One embodiment of the present disclosure is a method of directly transforming a sunflower from a mature seed with a heterologous polynucleotide, comprising: generating an explant from a germinated sunflower seed by removing cotyledons and primary leaves having intact meristem and tender shoot radicles; contacting the sunflower explant comprising the cotyledon with Agrobacterium comprising a heterologous polynucleotide in an infection medium supplemented with a plant hormone using vacuum and ultrasound treatment; co-culturing the explant from a germinating sunflower plant in a culture medium lacking the plant hormone in the presence of the Agrobacterium genus; culturing the mature seed explant in a regeneration medium comprising a selective agent; and culturing the seed with the regenerated tender shoots and with the secondary roots in a culture medium lacking the selective agent.

The invention discloses an application of a CsESE3 gene or a protein coded by the CsESE3 gene in regulation and control of plant shoot development, and belongs to the technical field of plant genetic engineering. Traditional citrus crossbreeding is long in period of cultivating ideal plant type plants, low in selection efficiency and few in selectable cultivation characters. Manual shoot control is high in labor cost, and chemical shoot control has the risk of phytotoxicity. Aiming at the technical problems, the invention provides a new scheme for regulating and controlling the development of citrus branch tips based on CsESE3 gene expression: the development of the branch tips is obviously promoted after the expression of the CsESE3 gene is improved, so that the CsESE3 can become a target spot for regulating and controlling the development of the citrus branch tips.

The invention provides a tissue culture method and an acclimatization and transplantation method of coptis chinensis, and belongs to the technical field of plant tissue culture. The tissue culture method of the coptis chinensis comprises the following steps: (1) taking a tender shoot of a coptis chinensis rhizome as an explant, sterilizing, and inoculating into a basic culture medium for culturing, so as to obtain a coptis chinensis sterile seedling; (2) inoculating the aseptic seedlings of coptis chinensis into an adventitious bud differentiation culture medium for culture to obtain adventitious buds of coptis chinensis; (3) inoculating the coptis chinensis adventitious buds into a proliferation culture medium for culturing to obtain coptis chinensis proliferation seedlings; and (4) inoculating the coptis chinensis multiplication seedlings into a rooting culture medium for culture to obtain coptis chinensis test-tube seedlings. The tissue culture and acclimatization and transplantation method provided by the invention can improve the propagation efficiency of the coptis chinensis, remarkably shorten the growth cycle of the coptis chinensis, provide technical support for rapid propagation and sustainable utilization of resources of the coptis chinensis, and have wide application prospects.

The invention provides a method for grafting Tibetan white bark pine on Chinese pine, belongs to the technical field of needle-leaved tree species grafting, and solves the technical problem that the survival rate is lower than 40% due to slow joint healing, high pathogen infection rate and environmental fluctuation. According to the technical scheme, the method is characterized by comprising the following steps: cutting 1.8-2.2 cm of a vertical notch reaching xylem at the part, which is 2.5-3.5 cm exposed out of the surface of a culture medium, of a rootstock rhizome part; cutting the scion base into a symmetrical double-sided wedge shape, and immersing the scion base into a sodium citrate buffer solution containing polyvinylpyrrolidone, methyl salicylate and calcium chloride; the two sides of the vertical notch are coated with adsorption glue; inserting a scion alignment cambium and fixing by using a grafting belt; an ethanol water solution containing salicylic acid, trehalose and polysorbate 80 is applied to the interface every 2-3 days; maintaining the day temperature to be 23-25 DEG C, the night temperature to be 16-18 DEG C and the humidity to be 90-95%; when the young sprouts grow to 2-3 cm, the rootstocks are girdled, and trunks are cut off after 7-10 days. The method is used for large-scale breeding of the Tibetan pinus bungeana grafted seedlings.

Compositions and methods for increasing the quantity of plant-based oil produced per hectare in a given time period are provided, wherein the compositions include the use of one or more fatty acids that kill or inhibit the growth of cells in the shoot apical meristem and one or more surfactants.

The application discloses a grafting seedling cultivation method for improving grafting survival rate, relates to the technical field of grafting seedling cultivation, and aims at the problem of poor success rate of seedling grafting. The application deeply fuses plant physiological characteristics, geometric morphological parameters and grafting mechanics principles, constructs a quantifiable and traceable method selection model, improves the adaptation efficiency of the grafting method by more than 3 times, realizes continuous optimization of the healing environment through continuous circulation of data comparison and parameter adjustment, guarantees efficient grafting process, sets evaluation indexes such as callus development, new shoot growth, environmental stability and root development, accurately judges the growth quality of the grafting seedling at different healing stages, realizes continuous optimization of the healing environment through continuous circulation of data comparison and parameter adjustment, and guarantees efficient grafting process.

Systems and methods relating to utilizing plant shoot-to-root temperature differentials alone or in conjunction gas mixture humidity about a plant shoot to improve plant bast fiber growth; the timing, sequence, and range of shoot-to-root temperature differentials selected during plant development utilized to modify and improve plant bast fibers for industrial, scientific, and medical use.

The invention discloses a composite agent for preventing and treating forest diseases and insect pests and a preparation method thereof, and relates to the technical field of forest disease and insect pest prevention and treatment. The composite agent is composed of a natural active extract, a synergistic auxiliary agent and a repair conditioning agent, the natural active extract comprises a melia azedarach fruit-leaf extract, a camellia seed meal extract, a pinus massoniana needle leaf extract and a camphor tree bark extract, and the composite agent has broad-spectrum insecticidal and bacteriostatic effects; a tea residue modified penetrant and early indica rice starch sustained-release microspheres are adopted as the synergistic auxiliaries, so that the adhesiveness and infiltration capacity of the pesticide on waxy leaves are remarkably improved, and long-acting sustained release of active ingredients is realized; the restoration regulator is moso bamboo vinegar, can promote the growth of new shoots of trees and the synthesis of chlorophyll, and enhances the stress resistance of the trees. All the components are synergistically prepared through a green process, the combined effect of multiple components is fully played, insect and disease prevention, long lasting period and ecological restoration integration are achieved, and an efficient and ecologically compatible novel plant protection technical scheme is provided for forest health management and sustainable development.

The application discloses a large-scale Chinese cherry seedling rapid shaping growth cultivation method, which comprises the following steps: S101, seedling selection and planting hole digging; S102, planting: single-hole double-planting V-shaped planting is carried out; S103, soil covering and pre-promotion management; S104, middle-promotion management I: when new shoots grow to 10 cm, amidoxydiphenylmethane is sprayed on leaves; S105, middle-promotion management II: amino acid water-soluble fertilizer is sprayed on leaves; and S106, daily management. The application has the beneficial effects that: the application realizes the dense planting cultivation for tourism development by means of single-hole double-planting V-shaped planting during the Chinese cherry seedling period, inoculation of Leptomitus fragilans agent through pre-promotion management during the seedling period, spraying of amidoxydiphenylmethane through middle-promotion management I and additional spraying of amino acid water-soluble fertilizer through middle-promotion management II, the seedling period of the Chinese cherry is shortened, and the requirement of rapid fruit setting is met.

The present application relates to a kind of fluoro-eth composition, plant growth regulator and its application, belong to the technical field of pesticide.The present application provides a kind of fluoro-eth composition, including flumetralin and ethephon, and the mass ratio of flumetralin and ethephon is flumetralin:ethephon=1:(0.05-20).The present application creatively combines control tip drug flumetralin and kill tip drug ethephon together to prepare fluoro-eth composition, and the compound preparation of flumetralin and ethephon can be used to control and inhibit litchi winter shoot, and its efficacy is mild, and the safe period is longer, and the safety is extremely high, compared with the conventional chemical control drug used alone, the control effect on litchi winter shoot is also more comprehensive and excellent, not only can control the germination of winter shoot, is conducive to the accumulation of tree nutrients, promotes flower bud differentiation, but also can have obvious control or kill shoot effect on the growth of litchi winter shoot that just sprouts certain length, so as to reduce the consumption of tree nutrients, lay a solid foundation for the stable yield of second year.

The utility model discloses a plant top end tender shoot removing device which comprises a removing unit, the removing unit comprises a rectangular box and a fixed cutter fixedly connected to one side of the rectangular box, the side wall of the rectangular box is provided with a limiting opening, the bottom of an inner cavity of the rectangular box is rotationally connected with a rotary disc, the side wall of the rotary disc is fixedly connected with a movable cutter penetrating through the limiting opening, and the movable cutter is fixedly connected with a rotating shaft; the top of the fixed cutter is fixedly connected with a sawtooth plate, the bottom of the movable cutter is fixedly connected with a sawtooth plate, the driving unit comprises a fixed plate fixedly connected to the top of the rotary disc, and the top of the fixed plate is rotationally connected with a movable plate. According to the utility model, the rotating disc can drive the movable cutter to deflect by pulling the pulling plate, and the tender shoots can be hooked through the sawtooth plate, so that the tender shoots cannot slide on the cutter edges of the fixed cutter and the movable cutter, the tender shoots can be conveniently cut off at one time, and the tender shoot removing efficiency is improved.

The present application relates to a kind of efficient breeding methods of high resistance larch new germplasm of pine moth, including larch high resistance scion collection, rootstock selection and processing, grafting breeding and grafting management etc., in the process of high resistance scion seedling breeding, by scientific seed winter storage, sowing seedling and seedling management etc., to obtain a large number of healthy high resistance scion tender shoot provides guarantee. Scion uses the tender shoot of 10-15 days growth on seedling, and the high adaptability larch seedling of 10-15 days seedling stem straight thick, top bud full, root system developed, no disease and injury is selected as rootstock, so that grafting joint can heal quickly, shorten breeding cycle. After grafting, first grow in the environment very stable plant growth room for about 2 months, etc. Scion survival, transition period is transferred into greenhouse and grows for 1 year to reach healthy seedling, finally, further seedling raising in outdoor open-air test ground, so that seedling is fully lignified, thus a large number of high resistance pine moth larch seedling resources for artificial afforestation are obtained. The present application reduces the probability of occurrence of insect pests from the source, improves the insect resistance of larch itself, and improves the forest quality, growth rate and wood utilization efficiency.

The application relates to the technical field of genetic engineering, in particular to application of CsCAT1 in regulating distribution of theanine between new shoots and leaves of tea trees, and to reduction of the stem / leaf ratio of theanine in tea trees by reducing the expression amount of CsCAT1 in tender stems, wherein the base sequence of the CsCAT1 is shown as SEQ ID NO. 1, the stem / leaf ratio of theanine in the tea trees is reduced, the content of theanine in tender leaves of the tea trees is increased, and the quality of tea leaves is further improved.

The present application relates to a kind of plum cultivation method of two harvests in a year, comprising the following steps: (1) culture plum spring shoot forms flower bud;(2) after two fruit harvest, water and fertilizer A is removed, summer pruning, control shoot;(3) remove leaf, winter pruning and apply base fertilizer;(4) root water and fertilizer B, germination;The flower of first fruit opens on the spring shoot;(5) in early February of next year, the flower of two fruit opens on the short fruit branch and flower bouquet fruit branch of the plum;4 in mid-April to mid-May, the first fruit matures;After the first fruit harvest, water and fertilizer C is removed, and the second fruit matures from mid-June to early July.The plum of the present application is two harvests in a year, and the total yield of two harvests (fruit) increases by 30.26%-52.50%, the first fruit matures 1-2 months earlier than normal, and the selling price increases by 3.29-4.36 times, the output value increases by 2.38-3.12 times, while adjusting the plum market supply in off-season, lengthening the shelf life of plum, improving the value of plum, thus better economic benefits are generated.

This invention relates to the field of rapeseed harvesting technology, and more particularly to an orderly rapeseed harvester for greenhouses. The harvester includes a harvesting frame equipped with a cutting blade, multiple sets of separating components, a first-direction conveyor belt mounted on the harvesting frame, an openable baffle, and a second-direction conveyor belt mounted on the harvesting frame. The multiple sets of separating components are movably connected to a frame installed at the front end of the harvesting frame, used to gather the entire rapeseed plant towards the center. The first-direction conveyor belt is used to transport the rapeseed cut by the cutting blade, and it is equipped with multiple sets of separating plates. This invention uses the separating components to gather each vegetable plant towards the center, making the stems and leaves of the rapeseed more compactly arranged. Then, the openable baffle limits the cut rapeseed, causing it to tilt onto the first-direction conveyor belt and be transported to the second-direction conveyor belt, where it is then transported again in the opposite direction, facilitating subsequent centralized collection and processing.

The invention discloses a flower and fruit dual-purpose pruning method for plum trees, and belongs to the technical field of fruit tree planting. The method comprises the steps of base fertilizer application and whole garden management in autumn and winter, first-time pruning and fertilization in a flowering period, second-time pruning and fertilization in a spring shoot stopping growth period, bud picking, pinching and fertilization in a summer shoot growth period, phosphorus and potassium fertilizer application after fruit picking and the like. According to the method, by adjusting the pruning opportunity and mode, sufficient flowering branches are reserved for appreciation in spring without pruning in winter, tree shapes are cultivated through multiple times of precise pruning after flowering, nutrients are saved, and the yield and quality are guaranteed by matching with whole-process scientific fertilization and nutrition supplementation; the method is simple to operate and easy to manage, the ornamental effect that the plum trees are full of flowers in early spring is guaranteed, the yield and quality of the fruits can be maintained, the dual value of ornamental value and fruit harvesting of the plum trees is achieved, and the method is suitable for plum tree planting in leisure agriculture.

The present application relates to the technical field of forestry asexual propagation, and particularly relates to a tissue culture and rapid propagation method of Salix psuedopectinata. The present application realizes the correction of the leaf color of Salix psuedopectinata tissue culture seedlings, solves the yellowing problem of the leaves in the process of Salix psuedopectinata tissue culture, and promotes the new shoots and rooting of Salix psuedopectinata, so that the Salix psuedopectinata tissue culture seedlings grow well. The method of the present application provides a new way for the preservation of the excellent germplasm resources of Salix psuedopectinata and the factory seedling.

This invention discloses a highly efficient propagation method for clump-forming bamboo based on pre-cultivated bamboo, relating to the field of clump-forming bamboo propagation technology. The method includes the following steps: selecting a nursery site, applying well-rotted farmyard manure as base fertilizer, drying it, then digging planting holes, spraying a bud-promoting agent solution onto the buds at the base of the mother bamboo culm, planting the mother bamboo seedlings with the culm petioles facing downwards, the shoots arranged on both sides, and the bamboo stumps placed obliquely in the planting holes, covering with soil in layers and compacting, watering, and managing the seedlings after planting; during the shoot growth period, diluting the bud-promoting agent solution and applying it along a circular trench around the mother clump, then covering with fallen leaves and keeping the soil moist, pruning the tops after new culms emerge; the following year, digging up the seedlings, leaving weaker seedlings, retaining part of the mother clump's bamboo stump, re-covering with soil, and allowing the mother clump to continue growing. The highly efficient propagation method for clump-forming bamboo provided in this application can improve propagation efficiency, shorten the seedling cycle, and effectively solve the problems of low propagation coefficient, unsustainable mother clump utilization, high seedling costs, and unstable seedling quality in existing technologies.

The application discloses a method for planting agaricus bisporus by using amomum tsao-ko stalks, and belongs to the technical field of edible mushroom planting. The amomum tsao-ko stalks, corn cobs, wild rice shoots and beer dregs which are soaked in a suspension liquid extracted from the amomum tsao-ko stalks and then fermented are used as planting substrates. After the agaricus bisporus mycelium grows on the planting substrates and covers the mushroom soil, trehalose and bamboo joint ginseng extract solution are sprayed, so that the agaricus bisporus mycelium germination can be effectively promoted, the mushroom speed can be improved, and the agaricus bisporus fruiting body yield and the agaricus bisporus cap diameter can be increased.

Device (1) for cutting branches and / or shoots of plants, comprising a cutting device (3) and a handle device (5), wherein the cutting device (3) is designed in two parts and has a first cutting head (7) and a second cutting head (9), and where the first cutting head (7) is arranged at a first distance from a handle (5) of the device and the second cutting head (9) is arranged at a second distance from the handle (5) is arranged, wherein the distance between the first and the second cutting head (7, 9) is variable, characterized in that an actuator (17) is included which is connected directly or by means of a connecting element to the first and / or the second cutting head (7, 9) such that the first distance of the first cutting head (7) and / or the second distance of the second cutting head (9) to the handle device (5) is variable, wherein the first cutting head (7) comprises n knife holders (23) and the second cutting head (9) comprises n-1 knife holders (25), with n = 2, 3, 4, 5, 6, 7, 8, 9 or 10 and the knife holders (23,25) of the first and second cutting heads (7,9) are arranged offset from each other.

The application discloses a water and fertilizer integrated tea tree plug seedling device and a seedling method, and steps are as follows: 1, mother tree treatment; 2, preparation of the device: the cutting substrate formula is grass charcoal: loess: residue: perlite; 3, shoot selection: selecting vigorous and pest-free current-year semi-lignified branches from the mother plant in a mother garden; 4, shoot cutting: pruning the collected branches into one growth point and half a leaf; 5, cutting: using a rooting agent; 6, rooting period management: the nutrient solution is a seedling technology matched with the cutting device, the substrate is continuously transported to the tea cutting root, the root is strengthened, and the seedling is strengthened; 7, seedling cultivation: the tea rooting nutrient solution is used in cooperation with the seedling device. The application also relates to a device, and capillary tubes are connected with the plug and a water storage device respectively. The water and fertilizer integrated management in the tea cutting seedling process is realized, the labor cost can be saved, the survival rate of tea is improved, and the application has application value.

Compositions and methods for improving one or more agronomic traits of a plant are provided, which include the use of one or more fatty acids that kill or inhibit the growth of cells in the shoot apical meristem and optionally one or more surfactants. Use of the composition between vegetative growth stage 1 (V1) and vegetative growth stage 6 (V6) is described.

The invention belongs to the technical field of agricultural biological control, and discloses a method for controlling summer shoots of Gannan navel oranges by insects, which comprises the following steps: S1, in the early germination stage of the summer shoots of the navel oranges in early May, putting 3-4 Acrida cinerea imagoes every 8m according to the projection area of a tree crown; s2, in the summer shoot germination peak period in the first ten days of June, the release amount of acrida cinerea is increased to 6-8 acrida cinerea per 8 m. By means of shoot control mechanism innovation, grasshoppers specifically gnaw young shoot growing points, side bud germination is prevented from being stimulated, and the vicious circle of'more and more wipe 'is blocked from the biological level; the shoot control efficiency is increased, the shoot control rate can reach 99.2% or above by the putting amount of 3-4 shoots per 8m, and the whole summer shoot growing period is covered continuously and periodically; the feeding rate of old leaves is strictly controlled to be 3.6% or below, the influence on the photosynthesis of a tree body is avoided, a precise shoot control technology is achieved based on biological habits, efficient and safe shoot control treatment is achieved, the survival conditions of diaphorina citri are worsened, the occurrence of the Huanglongbing is reduced, and the economic benefits of navel orange planting are improved.

This invention discloses a method for simultaneous flowering and fruiting of macadamia nuts. First, in September of the current year, pre-harvest fertilizer is applied to the 695 macadamia nut variety before fruit harvest. In October, when the fruit matures, 1 / 5 to 1 / 3 is harvested, with the remaining fruit left on the branches. Post-harvest pruning is carried out in October and November, leaving branches that did not bear fruit unpruned; harvested branches are lightly pruned. From November to March of the following year, the remaining fruit is harvested in batches, leaving no more than 1 / 4 of the fruit, with branch pruning performed simultaneously during harvest. Before flower bud differentiation in November, a flower-promoting fertilizer is sprayed to encourage flower bud differentiation. Around March of the following year, the 695 macadamia nut variety flowers, achieving simultaneous flowering and fruiting, with all remaining fruit harvested. This invention addresses the low natural fruit drop rate of the 695 macadamia nut variety by harvesting a portion of the fruit during the October ripening period, leaving the rest on the branches, and then harvesting in batches according to market conditions to stagger market entry. Simultaneously, the fruit is used to suppress the sprouting of autumn / winter and spring shoots, promote flower bud differentiation, and reduce the workload of post-harvest pruning.

The application discloses a tissue culture and rapid propagation method for Yangmei of Alocasia macrorrhiza and a special combined culture medium thereof, and the method comprises the steps of sterilizing an explant, carrying out primary culture and subculture and proliferation culture in stages, wherein the primary culture is performed by first inoculating a stem segment into an induction culture medium containing 6-BA 0.8-1.2 mg / L, IBA 0.05-0.15 mg / L and PVP-40 to induce germination, and then transferring the stem segment into an elongation culture medium containing 6-BA 0.1-0.5 mg / L, NAA 0.01-0.05 mg / L and PVP-40 to promote elongation; the new shoots are then transferred into a subculture medium containing 6-BA 1.0-1.8 mg / L, NAA 0.1-0.2 mg / L, GA3 0.5-1.5 mg / L and PVP-40 to proliferate. The application effectively solves the technical problems that Yangmei is prone to brown and death, and it is difficult to simultaneously achieve budding and high growth by means of two-stage hormone stepwise regulation and cooperation with a brown prevention agent, and realizes efficient and large-scale breeding of high-quality seedlings.

Compositions and methods for improving one or more agronomic traits of a plant are provided, which include the use of one or more active ingredients that are herbicides that kill, damage, or inhibit the growth of cells in the shoot apical meristem and optionally one or more surfactants, fungicides, bactericides, and / or pesticides. Use of the composition at specific vegetative growth stages of the plant, for example, between vegetative growth stage 1 (VI) and vegetative growth stage 6 (V6) is described.

This invention provides a green cultivation method for improving the quality and efficiency of low-value litchi orchards. The method includes collecting data on the existing variety retention status, planting density, canopy overlap rate, canopy shading rate, number of new shoots per tree, and proportion of diseased and insect-infested branches in the orchard to be renovated. Based on these indicators, the low-value type and renovation intensity level are determined. Renovation paths are matched according to variety renewal, canopy closure renovation, or mixed renovation types, using methods such as top grafting, thinning and pruning, or a combination of both. The planting density after thinning, pruning height, number of grafted branches per tree, and number of new shoots per tree are determined based on the renovation intensity level. After the grafted branches survive or the second batch of new shoots turns green, the number of shoots is counted, and re-grafting, re-pruning, or topdressing to promote shoot growth are performed based on the statistical results. This invention enables the renovation of low-value orchards to shift from experience-based processing to a continuous management approach combining classification, grading, and feedback correction.

The present application belongs to the technical field of plant cultivation, in particular to a method for inducing and regenerating seedlings from Heige callus. The method comprises the following steps: callus induction on Heige, callus regeneration induction culture to obtain regenerated shoots, and further subculture and rooting culture to obtain seedlings. The method improves the callus regeneration induction rate.

The invention belongs to the technical field of fruit tree cultivation, and particularly relates to a method for renewing and rejuvenating an old lemon tree body. The method comprises the steps that after fruit picking is finished, old lemon trees are trimmed, and new shoots are managed; young shoot management comprises the steps that 2-3 weeks after young shoots germinate from the trimmed old lemon trees, weak young shoots and young shoots with flowers are removed, and 3-5 strong branch shoots in different directions are reserved on each trunk or main branch; when the reserved branch tips grow to about 25-35 cm, pinching is carried out, and 6-8 leaves are reserved; and cyclically performing new shoot management on the trimmed and germinated treetops by adopting the treatment method until the tree vigor recovers to an expected effect. According to the method, the purpose of quickly recovering the tree vigor can be achieved by performing tip wiping management on young shoots (trimmed young shoots) with weak shoots removed and strong shoots reserved, reserving strong and vigorous young shoots, wiping weak shoots and young shoots with flowers, reducing nutrient consumption, promoting strong and vigorous growth of reserved branch shoots and combining deep ploughing of soil and a corresponding fertilization scheme.