The invention discloses an apple
rootstock tissue culture seedling raising method, and relates to the technical field of
tissue culture. The method comprises the following steps: (1) washing annual robust branches with tender shoots in a
dormancy period with running water, soaking with soapy water, cleaning, obliquely shearing into stem segments, soaking with
alcohol, washing, sterilizing, washing, absorbing
moisture, and shearing off wounds to obtain pretreated explants; (2) inoculating the pretreated explant into a differential culture medium, and culturing to obtain an adventitious
bud; (3) transferring the adventitious buds into a proliferation culture medium, and performing dark culture and light culture to obtain adventitious
bud clusters; (4) inoculating the adventitious
bud clusters into a rooting culture medium, and culturing to obtain
tissue culture seedlings; and (5) hardening the tissue culture seedlings, washing off the culture medium, disinfecting, putting into a culture medium, culturing to obtain apple seedlings, and
transplanting to outdoor soil. According to the method, the seedlings capable of being transplanted outdoors can be rapidly obtained, and the problems of infectious microbe
pollution, low
survival rate, poor rooting effect and the like in existing tissue culture
seedling culture are effectively solved.