49 results about "Shoot" patented technology

This invention discloses a highly efficient propagation method for clump-forming bamboo based on pre-cultivated bamboo, relating to the field of clump-forming bamboo propagation technology. The method includes the following steps: selecting a nursery site, applying well-rotted farmyard manure as base fertilizer, drying it, then digging planting holes, spraying a bud-promoting agent solution onto the buds at the base of the mother bamboo culm, planting the mother bamboo seedlings with the culm petioles facing downwards, the shoots arranged on both sides, and the bamboo stumps placed obliquely in the planting holes, covering with soil in layers and compacting, watering, and managing the seedlings after planting; during the shoot growth period, diluting the bud-promoting agent solution and applying it along a circular trench around the mother clump, then covering with fallen leaves and keeping the soil moist, pruning the tops after new culms emerge; the following year, digging up the seedlings, leaving weaker seedlings, retaining part of the mother clump's bamboo stump, re-covering with soil, and allowing the mother clump to continue growing. The highly efficient propagation method for clump-forming bamboo provided in this application can improve propagation efficiency, shorten the seedling cycle, and effectively solve the problems of low propagation coefficient, unsustainable mother clump utilization, high seedling costs, and unstable seedling quality in existing technologies.

The application discloses a tissue culture and rapid propagation method for Yangmei of Alocasia macrorrhiza and a special combined culture medium thereof, and the method comprises the steps of sterilizing an explant, carrying out primary culture and subculture and proliferation culture in stages, wherein the primary culture is performed by first inoculating a stem segment into an induction culture medium containing 6-BA 0.8-1.2 mg / L, IBA 0.05-0.15 mg / L and PVP-40 to induce germination, and then transferring the stem segment into an elongation culture medium containing 6-BA 0.1-0.5 mg / L, NAA 0.01-0.05 mg / L and PVP-40 to promote elongation; the new shoots are then transferred into a subculture medium containing 6-BA 1.0-1.8 mg / L, NAA 0.1-0.2 mg / L, GA3 0.5-1.5 mg / L and PVP-40 to proliferate. The application effectively solves the technical problems that Yangmei is prone to brown and death, and it is difficult to simultaneously achieve budding and high growth by means of two-stage hormone stepwise regulation and cooperation with a brown prevention agent, and realizes efficient and large-scale breeding of high-quality seedlings.

Compositions and methods for improving one or more agronomic traits of a plant are provided, which include the use of one or more active ingredients that are herbicides that kill, damage, or inhibit the growth of cells in the shoot apical meristem and optionally one or more surfactants, fungicides, bactericides, and / or pesticides. Use of the composition at specific vegetative growth stages of the plant, for example, between vegetative growth stage 1 (VI) and vegetative growth stage 6 (V6) is described.

The application discloses a kind of wild membrane pod astragalus rapid induction of adventitious shoots, which is by tissue culture to select wild membrane pod astragalus sterile seedling young stem as explant, inoculation is added in MS culture medium of different concentrations 6-BA, IAA and IBA to induce adventitious shoots.Adjusting the ratio of different hormones, the best induction condition suitable for wild membrane pod astragalus adventitious shoots is screened out, a set of method suitable for wild membrane pod astragalus rapid induction of adventitious shoots is established, the method of wild membrane pod astragalus induction of adventitious shoots is enriched, and the method is high and can be used for the induction of a large number of adventitious shoots, greatly shorten the wild membrane pod astragalus seedling cycle and reduce cost, lay a good foundation for wild membrane pod astragalus germplasm resource protection and seedling rapid propagation.

The useful solution refers to a naturally occurring chlorophyll-rich preparation for animal feed, in which the preparation has a high natural chlorophyll content produced from natural chlorophyll leaves, consisting of 70% by weight of dried chlorophyll leaves mixed with 30% by weight of dried chlorophyll young shoots. In this process, 70% of fresh bamboo leaves and 30% of fresh bamboo shoots by weight are dried using the heat of gas-powered heating elements in two stages: the first drying stage is carried out for 15 to 18 hours at a temperature of 50 to 60°C, and the second drying stage is carried out for 15 to 18 hours at a temperature of 30 to 40°C; followed by natural cooling and grinding to a predetermined size depending on the type of livestock.

This invention belongs to the field of native shrub seedling propagation technology, and discloses a system and method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions. The system includes a data processing platform, acquisition equipment, and control equipment. The data processing platform establishes habitat and germplasm archives corresponding to the same germplasm batch and sequentially completes germplasm dormancy treatment, staged seedling management, root culture, mycotoxin-seedling symbiosis, stress substrate integration, and planting feedback. The method generates qualified germplasm records and germination initiation records based on germplasm status and dormancy type. Seedling stage status records serve as the basis for initiating the next stage. Root culture, mycotoxin inoculation, stress pre-adaptation, and substrate transition are performed based on the habitat information of the target restoration plot. After planting, survival rate, greening time, new shoot growth, and overwintering survival rate are recorded in the habitat and germplasm archive to correct parameters for subsequent batches.

This invention provides a method for selecting plants that possess desired traits regarding the internode length of the main stem. [Solution] One aspect of the present invention is a selection method that includes a step of selecting plants using the length of the young shoot at the third leaf development stage as an indicator.

This invention provides a compound biological preservative for *Bambusa textilis* shoots, its preparation method, and its application, relating to the field of plant preservation technology. The compound biological preservative comprises, by weight percentage, 1.5%-2.5% chitosan, 0.02%-0.05% nisin, 3%-5% ascorbic acid, and the balance being water. This invention overcomes the shortcomings of existing technologies, effectively delaying the deterioration of the appearance and quality of *Bambusa textilis* shoots, alleviating their fibrosis, and extending their shelf life.

A method for increasing yield or vigor of a plant is provided, which includes the selective removal of the plant's shoot apical meristem at a period between vegetative growth stage 1 and vegetative growth stage 2, or between vegetative growth stage 2 and vegetative growth stage 3.

This invention relates to a method for preparing a fern-like beverage. Fern-like fern (Pteris tigrinosa) is a plant belonging to the genus *Pteris* in the family Pteridaceae. The young leaves of *Pteris tigrinosa* are edible because they have a refreshing cucumber-like aroma, hence the common name "cucumber-scented fern." The purpose of this invention is to provide a method for preparing a high-quality fern-like fern beverage, taking advantage of the fern's rich content of various beneficial components and its cucumber-like aroma. The preparation method involves adding taste-adjusting ingredients to water and mixing thoroughly. Cleaned young fern shoots, processed to a size of 1 mm to 10 mm, are then added to the solution. The mixture is mixed thoroughly, bottled, and the bottled fern-like fern beverage is sterilized in a water bath at 70℃-100℃ for 30 minutes to obtain the finished product. This invention is beneficial for the development and utilization of fern-like fern in beverages.

The present application relates to economic crops planting technical field, disclose a kind of selection and breeding method of green pricklyash tree fruiting branch, the method is to select the new shoot of green pricklyash tree in fruit swelling stage as the fruiting branch of next year, and cooperate pruning, fertilization and the multiple action of plant growth regulator composition, stimulate the formation of latent bud, latent bud germination in fruit swelling stage, increase the number of new shoots, promote the growth of new shoots, so as to ensure that the number of new shoots is greater than the required number of fruiting branch, and the length of new shoot aging branch reaches more than 100 centimeters of the demand of high yield.The selection and breeding method provided by the present application selects the new shoot of green pricklyash tree in fruit swelling stage as the fruiting branch of next year, the growth time of new shoot is more than 150 days and the water and heat is sufficient in growth period, compared with the growth time of new shoot in water and heat sufficient area, the growth time is longer, and it is easier to age into flower;And the method is not limited by water and heat conditions, suitable for application and popularization in many areas.

The present application belongs to the technical field of apple planting, and discloses a method for preventing and controlling apple bitter scab, which comprises the following contents: (1) nitrogen fertilizer regulation, adopting a strategy of applying fertilizer in stages, including application of base fertilizer in autumn, application of topdressing fertilizer in spring, and nitrogen limitation management in summer; (2) new shoot regulation, setting differentiated new shoot growth thresholds according to the age stages of fruit trees; (3) fruit regulation, controlling the fruit retention position and amount through thinning flower and fruit management, and controlling the fruit size within a preset horizontal diameter range; (4) whole-cycle calcium supplementation, establishing a three-dimensional calcium supplementation system including foliar spraying in the growing season, root zone application, and post-harvest treatment. The present application effectively guarantees the quality and yield of apples, reduces the influence of bitter scab on the growth and development of fruit trees, thereby realizing high-quality, high-yield and high-efficiency of apples, and promoting the high-quality development of the apple industry.

The application belongs to the technical field of insect resistance research, and discloses a method for enhancing the insect resistance of poplar by using a leaf senescence related gene NAC109, which is characterized by comprising the following steps: (1) obtaining a CDS sequence of a PtNAC109 gene; (2) designing a gRNA sequence; (3) constructing a targeted PtNAC109 gene editing vector plasmid; (4) preparing a genetic transformation agrobacterium infection solution; (5) using agrobacterium to infect poplar plants; (6) screening resistant callus; (7) inducing, prolonging and rooting the resistant shoots; (8) verifying the gene editing effect by using molecular biology means; and (9) obtaining a new poplar gene editing germplasm with enhanced insect resistance.

The invention concerns a method for producing a shoot of a plant comprising germline progenitor cells of a recalcitrant plant. The germline progenitor cells may be modified to comprise a mutation in a sequence of interest. The invention further pertains to plants obtainable by the method of the invention, wherein the plant preferably comprises at least the L2-meristem layer of the recalcitrant plant.

The present application belongs to the technical field of economic crop biological prevention and control, and provides a kind of plant source attractant of aleurocanthus spiniferus and its application.The attractant composition of aleurocanthus spiniferus adult is composed of one of farnesene and leaf alcohol and linalool.The attractant composition is placed at tea shoots during the emergence of overwintering generation and last generation of aleurocanthus spiniferus adult, which can efficiently attract aleurocanthus spiniferus adult.The above-mentioned attractant composition can be prepared into preparation forms such as lure core, and can also be used with yellow stick trap.The attractant composition provided by the present application can reduce the number of insects throughout the year from the root source, and can reduce or avoid the use of chemical agents.The present application is beneficial to protect the population ecology of tea garden, has the characteristics of green, safe and efficient, is convenient and fast to use, has low cost, and is suitable for practical production practice.

This invention discloses a method for introducing exogenous genes into Gynostemma pentaphyllum, comprising: S1: Selecting Gynostemma pentaphyllum seeds with seed coats within 3 months of harvest, disinfecting, and culturing to obtain aseptic seedlings from seedlings, or selecting stem segments with axillary buds after leaf removal, disinfecting, and culturing using a subculture proliferation medium containing cephalosporin to obtain aseptic rooted seedlings; S2: Selecting the aseptic seedlings or aseptic cuttings obtained in S1, culturing in a rooting medium containing cephalosporin to obtain aseptic rooted seedlings; S3: Transforming plasmid Pcambia1301-PMI into Agrobacterium and culturing in a medium containing kanamycin and rifampin to obtain an Agrobacterium resuspension; S4: Infecting the aseptic rooted seedlings obtained in S2 with the Agrobacterium resuspension, and performing co-culture, antibacterial culture, and screening culture to obtain transgenic adventitious shoots. This invention can successfully introduce exogenous genes into Gynostemma pentaphyllum, obtaining Gynostemma pentaphyllum positive plants.

This invention discloses a promoter, ProWUS, specifically for the apical meristem of hybrid tulip tree and its applications, belonging to the field of plant genetic engineering technology. Based on the genome sequence information of Chinese tulip tree, the ProWUS promoter sequence was cloned, and its nucleotide sequence is shown in SEQ ID NO.1. Driven by the cloned ProWUS promoter, the reporter gene can be specifically expressed at the apical meristem of the plant. Using the promoter provided by this invention, the specific expression of the corresponding target gene in the apical meristem can be induced in plant genetic engineering, which not only enriches the molecular biological theoretical basis of plant shoot tips but also has important application value in the development of plant apical meristems and the maintenance of stem cells.

This invention discloses a plant rhizosphere growth-promoting bacterium, *Aristolochia nitroguaiacos* (…). Paenarthrobacter nitroguajacolicus YD-2-4, deposited at the China Microbial Culture Collection Center (CGMCC) under accession number CGMCC No. 36649, possesses various growth-promoting properties, including nitrogen fixation, phosphorus solubilization, IAA production, and ACC deaminase production. It significantly promotes the growth of forage grasses such as alfalfa, wheatgrass, crested wheatgrass, red fescue, Artemisia arenaria, and Stipa purpurea. The strain promoted shoot growth by 25.98% and 14.85% in Artemisia arenaria and red fescue, respectively, and root growth by 27.98% and 19.85% in Artemisia arenaria and Stipa purpurea, respectively. It also increased biomass accumulation in Artemisia arenaria and red fescue by 36.60% and 22.91%, respectively. Regarding plant antioxidant capacity, YD-2-4 significantly enhanced peroxidase activity in Artemisia arenaria and alfalfa, and increased the total antioxidant capacity of red fescue and Stipa purpurea. Strain YD-2-4 can serve as an excellent plant rhizosphere growth promoter, and has broad application prospects in the microbial remediation of degraded grasslands in alpine regions and the development of biofertilizers.

Provided herein are methods, apparatuses, computer program products, and systems for seed quality analysis using computed tomography. One method can include obtaining computed tomography data representing a germination towel including seedlings; detecting likely seeds and candidate shoots and roots in the computed tomography data representing the germination towel; forming segments from the candidate shoots and roots; grouping the segments across a total volume of the computed tomography data into likely shoots and roots in accordance with relative proximity and alignment of the segments; assembling the likely seeds and the likely shoots and roots into likely seedlings; classifying the germination towel using a classifier trained to use at least the likely seedlings, as input; and providing a classification result from the classifying for the germination towel.

This invention belongs to the field of plant tissue culture and rapid propagation technology. A method for establishing a rapid propagation system for *Symplocos buergeriana* includes inducing shoot growth using *Symplocos buergeriana* stem segments as explants, inducing and proliferating axillary buds on the stem segments, inducing callus tissue, inducing adventitious bud differentiation from callus tissue, and cultivating adventitious bud roots. The stem segment axillary bud induction and proliferation employ an integrated culture process, enabling directional induction of axillary bud germination and mass proliferation of clustered axillary buds without the need to change the specialized proliferation culture medium. Axillary bud differentiation and propagation are completed sequentially, rapidly obtaining a large number of robust clustered axillary buds. This invention's method for establishing a rapid propagation system for *Symplocos buergeriana* screens out an optimal culture medium formula and plant growth regulator ratio that is not limited by genotype differences and is universally applicable. It establishes for the first time a complete, stable, genotype-independent, highly versatile, and highly efficient tissue culture regeneration system for *Symplocos buergeriana*, providing reliable technical support for the protection of *Symplocos buergeriana* germplasm resources, large-scale rapid propagation of superior germplasm, and industrialized seedling production.

This invention discloses a medicament for inhibiting the formation of thorns in Sichuan pepper, relating to the field of Sichuan pepper cultivation technology. The medicament comprises the following components at final concentrations: lovastatin 5-20 mg / L, 6-mercaptopurine 5-10 mg / L, pyrimidinol 10-30 mg / L, proline 20-100 mg / L, glycine 20-100 mg / L, glutamic acid 20-100 mg / L, potassium dihydrogen phosphate 5-7 mg / L, sucrose 5-7 mg / L, and Tween-80 1-2 mg / L. This invention also provides a method for preparing the medicament for inhibiting the formation of thorns in Sichuan pepper. This invention reduces the incidence of thorns in Sichuan pepper, with a significant thorn-inhibiting effect. While inhibiting thorn formation, it also increases the growth of new shoots in Sichuan pepper, effectively solving the problem that single-hormone inhibitors easily lead to excessive shoot growth, decreased flower bud differentiation rate, or reduced fruit weight.

This invention relates to a tissue culture method for *Ligustrum lucidum* and its application, belonging to the field of plant tissue culture technology. The tissue culture medium combination for *Ligustrum lucidum* of this invention includes a sterile seedling solid-phase medium, a differentiation-inducing medium, a shoot-inducing medium, and a rooting medium. The *Ligustrum lucidum* plants obtained by this invention have a longer culture period, exhibit full growth and more mature development, robust root systems, and significantly improved stress resistance and environmental adaptability. After hardening-off treatment, the *Ligustrum lucidum* plants can gradually adapt to changes in external light, humidity, and temperature, and the final hardening-off survival rate after transplanting is high, providing important technical support for the rapid propagation and further optimization of genetic transformation technology for *Ligustrum lucidum*.

A new cultivar of Juniperus conferta plant named ‘KA5’ that is characterized by its dense and compact plant habit, its light yellow young shoots and mature green leaves, and its moderate growth rate.

This invention discloses a SNP molecular marker related to the density of pubescence on new shoots of tea trees and its application, belonging to the field of molecular marker technology. This invention develops two SNP molecular markers related to the density of pubescence on new shoots of tea trees: (1) the SNPtri1 molecular marker, whose nucleotide sequence is shown in SEQ ID NO.1. An A / G base polymorphism exists at the 101st base position in this sequence. When the polymorphic site is the AG genotype, it indicates a tea tree with high pubescence density; (2) the SNPtri2 molecular marker, whose nucleotide sequence is shown in SEQ ID NO.2. An A / G base polymorphism exists at the 101st base position in this sequence. When the polymorphic site is the AG genotype, it indicates a tea tree with high pubescence density. Using the molecular markers provided by this invention, tea tree germplasm resources with different pubescence densities can be accurately identified or screened during the seedling stage, possessing significant breeding application value.

This invention discloses an effective method for controlling leaf curling in macadamia nuts, comprising the following steps: selecting mineral oil with an effective ingredient content ≥99%, mixing it with water at a volume ratio of 1:100-400, then adding an emulsifier accounting for 0.05%-0.2% of the total weight of the mixture, stirring evenly to prepare a mineral oil emulsion; applying the emulsion using a misting device based on the growth characteristics of macadamia nuts during the shoot emergence period, pre-flowering period, young fruit stage, and mid-fruit development stage, combined with the differences in insect resistance among different varieties; integrating four measures: pruning and shaping control, orchard sanitation, soil regulation, and nutrient enhancement; and regularly monitoring key indicators such as thrips population density, leaf curling rate, and deformed fruit rate using a five-point sampling method, adjusting the control plan based on quantitative data. This invention achieves highly efficient control of thrips, prevents leaf curling, improves macadamia nut yield and quality, and protects the orchard ecological environment by optimizing the mineral oil emulsion formulation, precise application strategies, synergistic agricultural management measures, and dynamic monitoring and adjustment.

The application discloses application of CsESE3 gene or a protein coded by the same in regulation of branch development of plants and belongs to the technical field of plant genetic engineering. Traditional citrus hybrid breeding for cultivating ideal plant type plants is long in period, low in selection efficiency and few in cultivatable traits. Artificial control of branches is high in labor cost, and chemical control of branches has the risk of drug damage. The application proposes a new scheme for regulating citrus branch development based on CsESE3 gene expression, that is, the expression of CsESE3 gene is improved to significantly promote the development of branches, so that CsESE3 can become a target for regulating the development of citrus branches.

The present application provides a kind of kiwi fish bone shape pruning method, belong to the technical field of kiwi cultivation management.The method includes the directional cutting of the blade edge cutting into the inclined angle of 30° to 60° on the permanent main vine of the mother branch to be updated during the dormant period;Remove redundant sprouts in spring;After the target new shoot enters the initial stage of lignification and color change, implement the angle binding, so that the base forms a horizontal angle of 60° to 120°.During the growing season, summer pruning and phased water and fertilizer management are carried out on the target new shoot.The present application forms process control in the direction regulation of callus growth, wound liquid water diversion and lignin induction deposition at the base of new shoots, eliminates the conditions for abnormal shedding of new shoots and longitudinal migration of pathogens, and ensures the long-term health of the plant and the continuous stability of the structure.

The application discloses a method for promoting adventitious shoot regeneration of sunflower in vitro, and belongs to the technical field of plant tissue culture. The method comprises the following steps: taking sunflower 'YK86' sterile seedling cotyledon as an explant, inoculating the explant into a callus induction medium containing 0.01 nM exogenous HaREF1 small peptide and a bud differentiation medium in sequence after pretreatment, effectively promoting sunflower callus induction and adventitious shoot differentiation and regeneration, reducing the browning rate, and successfully inducing adventitious shoots. Through screening of the genotype and explant of sunflower in vitro regeneration, in combination with the synergistic effect of screening and adding different concentrations of HaREF1 to the callus induction medium and the bud differentiation medium, the application effectively promotes the differentiation and regeneration of sunflower adventitious shoots in vitro, solves the problems of difficult sunflower in vitro regeneration, low regeneration efficiency and instability, and provides technical support for sunflower genetic transformation, gene function verification and variety improvement.

The invention relates an intergrafting tool (1) for supporting a plant during an intergrafting process and to a method of generating a shoot of a plant using the same. The intergrafting tool (1) comprising a body (12) extending in a first direction (X); a retaining portion (14) projecting frontally from the body (12) and including at least two opposing retaining elements (15a, 15b) formed by two parallel bars extending in the first direction (X), and positioned next to each other in a second direction (Y) substantially perpendicular to the first direction (X), the at least two opposing retaining elements (15a, 15b) being configured to at least partially surrounding a stem of the plant and apply a gripping force thereto to secure the plant to the body (12); and an intergraft seat (16), located at a central region of the retaining portion (14) and providing frontal and lateral access to an interior of the retaining portion (14), thereby exposing a stem segment when the plant is secured to the body (12). The invention further relates to a method of generating an intergraft using the intergrafting tool and to an intergrafting tool array comprising said intergrafting tool.