616 results about "Cotyledon" patented technology

The invention relates to a method using the cotyledon node of soybeans as explant to perform soybean organogenesis and plant regeneration, belonging to the technical field of agricultural production. The invention is characterized in that based on the traditional tissue culture, the cotyledon node of soybeans is used as explant to perfect the regeneration system of organogenesis means. The invention has the advantages that based on the selection of effective disinfection technology for soybean seeds, the improvement and the optimization of various medium compositions is outstanding; through the verification of the tissue culture of conventional soybean varieties, the induction, the elongation and the rooting of multiple shoot are effectively promoted; the quality of aseptic seedlings and the differentiation rate of the multiple shoot are improved; the organogenesis and plant regeneration process of the cotyledon node of majority varieties are perfectly realized; solid technical support is provided for the genetic transformation and the molecular improvement of soybeans; and the invention has important practical significance to the effective breeding and health production of the soybeans in China.

The invention discloses a method for grafting watermelons and cultivating grafted seedlings. The method comprises the following steps of: soaking seeds and accelerating germination: soaking stock seeds and scion seeds with clear water and carrying out moisturizing and germination acceleration for a certain time by a sterilizer; sowing and growing seedlings: horizontally placing and carrying out dibble seeding on the stock seeds subjected to germination acceleration in a nutrition cup, carrying out dibble seeding the scion seeds into a seedling-raising disk and cultivating the scion seeds in a greenhouse, wherein the scion seeds are seeded later than the stock seeds for 7-10 days; grafting: when a first rough leaf of the stock seeds is fully extended and two leaves of the scion seeds do not horizontally extend, grafting scions by adopting a top piece inserting method; and cultivating the grafted seedlings, strictly maintaining certain cultivation conditions and controlling insect pests at regular period. According to the method disclosed by the invention, the grafting survival rate is improved to be over 90 percent. In addition, nutrient soil is prepared from mud in a fish pond as a basic raw material, so that growing quality and grafting survival rate of the stock seedlings, the scion seedlings and the grafted seedlings are effectively improved.

The invention discloses a method for grafting of a cucumber on a black-seed pumpkin in Yunnan, belonging to the field of crop breeding. According to the method, the black-seed pumpkin in Yunnan is used as a stock, a growing point and axillary buds on two sides of the stock are removed by a blade, a special bamboo stick of 0.5cm is vertically inserted into the center of the growing point and is maintained in a stem, a cucumber seedling is taken, the under-cotyledon part is removed by obliquely cutting at 0.4-0.6cm under the cotyledon of the cucumber seedling for an angle of 25-30 degrees, the bamboo stick is taken out, the cucumber seedling is inserted into a grafting hole, and the cotyledon of the cucumber and the cotyledon of the stock are arranged in a cross-shaped vertical shape; and after grafting is performed, moisture is kept for 3-5 days in an enclosed environment in a greenhouse, the temperature is maintained at 25-30 DEG C at daytime and 18-20 DEG C at night for seedling hardening, then ventilation is performed gradually, and complete ventilation is performed on the ninth or tenth day. The black-seed pumpkin in Yunnan is used as the stock in the method, diseases which can easily occur to certain cucumber seedlings can be eliminated completely, and the stress tolerance and disease resistance can be better improved. Since direct insertion is adopted for grafting, the rate of incidence of adventive roots is very low, and the survival rate of grafted seedlings can be guaranteed to be up to 98 percent.

The invention discloses a technology for culturing a fava bean sprout by the adoption of artificial light source, belonging to the technical field of biology. Fava bean seeds with a same size are selected, the seeds are soaked firstly and then taken out for drying, and the seeds are placed in a sprout culture disc for germination. After cotyledons are unfolded, the sprout culture disc is placed in monochrome red light and blue-red combined light until the sprout grows. The monochrome red light and blue-red combined light (B:R=6:4) is most suitable for the growth of cabbage type rape sprout. The technology has the advantages of simpleness in operation, energy saving, environment friendliness and the like, and can be applied in fields of indoor rapid growth, industrial sprout culture and the like for the fava bean sprout.

The invention relates to the field of plant physiology, in particular to a method for screening saline-alkaline resistant/tolerant cotton. The method comprises the steps that (1), a cotton culture solution is prepared, and the pH value of the cotton culture solution is adjusted between 6.5 and 7.0; (2), the culture solution serves as a solution to prepare NaCl solutions with the concentration being 0.0 wt%, 0.1 wt%, 0.2 wt%, 0.3 wt%, 0.4 wt%, 0.5 wt%, 0.6 wt% and 0.8 wt%; (3), a sand sprout method is adopted, when cotton seeds sprout and grow until all cotyledons are spread and turn into green and no root is generated, the cotton seeds are moved in the NaCl solutions; (4), cultivation is carried out in a shading condition; (5), in the ninth to tenth day, seedlings are taken out for surveying the root hair number of each cotton seedling, and the saline-alkaline resistant/tolerant degree of cotton is judged according to the number of the root hairs. The method excludes the interference of the factors that salinity gradient is uneven and rainfall wets salt in the field identification process, the duration is short, the identification result is stable and reliable, and method is high in practicability and maneuverability.

The invention discloses a production technology of a peanut butter, which belongs to the field of food processing. The production technology comprises the following steps of: baking: baking and heating peanuts to 160 DEG C, maintaining for 45-55 minutes till the inner color and outer color of peanut kernels are consistent, and selecting the peanuts without burnt taste for standby; removing peels and screening: timely discharging the baked peanut kernels, spreading for cooling or blowing cold wind for cooling, and cooling the peanut kernels and removing the peels by using a peeling machine and screening to separate seed leaves and embryos; milling to obtain the butter: putting the separated seed leaves in a grinding machine for milling to obtain the butter; canning and sterilizing: filling cans with the peanut butter, carrying out microwave sterilization on the peanut butter in the cans, and simultaneously cooling the tans by flowing water till the water level is in the range of one half to three quarters of the cans, wherein the whole process lasts for 10 minutes; and freezing: to standing the cans at the condition of -3 DEG C to -1 DEG C, and storing after completing whole crystallization. The production technology has the beneficial effects of being safe and sanitary, good in sterilization effect and simultaneously being capable of maintaining the original taste of the peanut butter.

The invention discloses a herbal composite and a preparation thereof for treating gynecological inflammation. The herbal composite is prepared of cotyledon, selinum, sophora, phellodendron, radix Stemonae, agrimonia, sanqi, borax, borneol; the weight ratios are that 3-15 portions of cotyledon, 3-15 portions of selinum, 3-15 portions of sophora, 2-10 portions of phellodendron, 2-10 portions of radix Stemonae, 2-10 portions of agrimonia, 0.2-2 portions of sanqi, 3.03-0.3 portion of borax, and 0.06-0.6 portion of borneol. The preparation can be suppository, gel, cream, solution agent, film, spray, effervescent tablets agent, and other externally used preparations. The invention adopts common herbal medicines as materials, the usage cost of a sufferer is lower, the content of the effective components are high, the stability is high, and the curative effect is notable. The herbal composite and the preparation of the invention can reduce fever, destroy the toxic properties, dry dampness, kill insect, remove rot, and produce fresh, can inhibit and kill pathogenic micro-organisms and infusorium, can improve the immunity of organism, can cure tickle and relieve pain, can accelerate metabolism, can promote the tissue to rebirth. The prescription is applicable for the sufferers with leukorrhea with reddish discharge, pruritus vulvae, cervical erosion, infusorium vaginitis, mucedine vaginitis, nonspecific vaginitis, etc.

The invention belongs to the technical field of plant processing and in particular relates to a method for improving the bioavailability of iron and zinc elements of broad beans. The broad beans are low in cost, contain abundant microelements and proteins and have a certain advantage to dietary nutrition of people. Phytic acid which is contained in cotyledons of the broad beans can be combined with proteins and metal ions to reduce the bioavailability of the proteins and the metal elements. The deficiency of iron and zinc elements has certain universality, and the iron and zinc elements play an important role in intelligence development of children and physical health of adults. Most people take vegetarian diet as staple food in China, wherein the broad beans are popular bean crops, and carrots and spinaches are popular vegetables. The carrots and spinaches contain beta-carotene; and according to the method disclosed by the invention, the fact that the bioavailability of the iron and zinc elements in the broad beans can be improved by adding the carrots and spinaches into raw broad bean flour or cooked broad bean flour is discovered by using the carrots, spinaches and the broad bean flour to do an in-vitro digestion experiment, and therefore the nutritional value in use of the broad beans is increased.

The present invention relates to herbicide composition of monosulfuron ester compound as well as its application, which is compounded with two-element or three-element from herbicide monosulfuron ester or its natrium salt (0.1 to 90 percent) as main active component, and other herbicide (0.1 to 85 percent) of ethyl carboxylic fluorine grass ether, Starane, thifensulfuron-methyl, tribenuron-methyl, 2,4D as well as its ramification, Prometrex prometryn, acetochlor, atrazine, triallate, metolachlor, bensulfuron-methyl or 2 armour 4 chlorin natrium etc., and the herbicide composition can be made into breast oil, wettable powder, soluble powder, water emulsion, microemulsion agent, aqua, suspending agent, microcapsule agent or water disperse particle agent by adding proper amount auxiliary agent and carrier accepted in agriculture. The composition can be used to prevent single and double cotyledon weed in wheat, corn and millet field.

The invention discloses a rapid propagation method for suspension cell culture of Millettia speciosa Champ. According to the rapid propagation method, young and tender cotyledons taken from Millettia speciosa Champ pods 50-60 days after flowering are utilized as culture materials, subcultured callus tissue is obtained through inducing culture and subculture, and a stable Millettia speciosa Champ suspension cell culture fluid is obtained through the suspension cell culture. With the adoption of the method, stable Millettia speciosa Champ suspension cells with good quality and high propagation yield can be obtained, and a novel way and method are provided for development and utilization of effective components of the Millettia speciosa Champ and creation of new germplasm resources from the Millettia speciosa Champ with biotechnology methods such as somatic hybridization, genetic transformation, mutant induction and the like.

The invention discloses a light source control technology for indoor culture of black soybean sprouting vegetables, and belongs to the technical field of biology. The light source control technology comprises the following steps that full black soybean seeds in uniform size are selected and are firstly placed into a seedling culture disc for germination acceleration; after the cotyledon grows flatly, the seedling culture disc is placed under a blue, red and yellow combined light LED (light emitting diode) to be cultured until the sprouting vegetables grow up. The blue, red and yellow combined light (B:R:Y=5:3:1) is most suitable for the growth of the black soybean sprouting vegetables. The light source control technology has the advantages that the operation is simple, the energy sources are saved, the environment is protected, and the like. The light source control technology can be applied to the fields of indoor fast production, factory seedling culture and the like of the black soybean sprouting vegetables.

The invention belongs to the technical field of pharmaceuticals, and relates to an artificial schisandra chinensis seed preparation and seedling culture method. The method can be used for normally germinating artificial schisandra chinensis seeds in a bacterial culture medium. The artificial schisandra chinensis seeds can be prepared according to the following process: inoculating excellent schisandra chinensis explants into a callus induction medium so as to obtain callus, carrying out embryonic type callus induction on the callus, carrying out somatic embryo induction on the embryonic type callus, synchronously culturing and subculturing somatic embryos obtained through induction, carrying out ion exchanging on cotyledon phase somatic embryos so as to form sodium alginate-CaCl2 hydrogel spheres, and wrapping the obtained hydrogel spheres by utilizing deacetylation chitosan and carbendazol micro powder so as to obtain the artificial seeds wrapped by artificial seed coats; and germinating the artificial seeds so as to obtain the schisandra chinensis. The method can be used for greatly culturing seedlings in a short time and is labor-saving and material-saving.

The invention relates to the cold resistance appraisal of plants, and particularly provides a cold resistance appraisal method of a cotyledon period. The cold resistance appraisal method of the cotyledon period comprises the following steps: carrying out low-temperature processing on the strong seedlings of the cotyledon periods of a plurality of strains to be tested, wherein the plurality of strains exhibit even growth vigor; then, causing the strains to recover growth under a normal temperature; investigating the states of stems and a situation that cotyledons are damaged; judging a cold damage level according to a standard; calculating the cold resistance index of a variety to be tested; and appraising the cold resistance level according to the standard. The appraisal method disclosed by the invention has the advantages of being simple, visual, reliable and short in periods, can effectively carry out the cold resistance screening of cotton seedling stage germplasm resources and varieties, and paves the way for the further research of the cold resistance breeding and the cold resistance mechanism of the cotton.

According to a mechanical assembly drum assisting turn-up of tire embryo seam allowance and a method for realizing assisted turn-up provided in the invention, rubber ring structures capable of swelling when inflated are arranged at the interior and exterior side of the tire embryo seam allowance and form into capsules after inflation to realize internal and external extrusion to the part of seam allowance, thereby improving quality of turn-up and compaction of the tire embryo seam allowance. The invention enables the operation of wrapping and compacting directed to the part of seam allowance to be realized and bubbles in the interior and exterior side of the tire embryo seam allowance to be completely eradicated, thereby substantially enhancing quality of turn-up of the part of the seam allowance. The mechanical assembly drum has a hollow cylindrical main shaft and a driving shaft which drives a locking piece assembly and a finger type turning up means for reciprocating motion; a rubber ring, which is connected with a locking piece unit and forms a closed ring structure around the periphery of the main shaft, is provided on the top of a locking piece cylinder body; two ends at the exterior side of the rubber ring are respectively fixed on the interior and exterior side of the locking piece cylinder body; a breather pipeline of the locking piece cylinder body communicates with the interior of the rubber ring. When the rubber ring is inflated, internal and external capsules are formed for direct wrapping, compacting and venting of the part of the seam allowance.

The invention discloses a method for controlling excessive growth of cucumber seedlings by using a light-emitting diode (LED) light source and belongs to the field of plant cultivation. The method comprises the steps of: when the cotyledons of seedlings come out of soil, supplementing light through LED plant growth lamps, wherein the light supplementing time is 8-16 hours per day and the intensity of supplemented light is 90 to 240mu mol.m<-2>.s<-1>; and when three leaves and one core grow out of each seedling, stopping supplementing the light. The method for controlling the excessive growth of the cucumber seedlings by using the LED light source has the advantages that the defects of the traditional water-controlled seedling raising method and the method of using growth inhibitors to regulate the excessive growth of the seedlings are overcome, the effect of the red and blue LED light source capable of controlling the excessive growth of plants is remarkable, the method is safe and environmental-friendly, the service life of the LED lamps is long and the LED lamps are economic and affordable.

The invention discloses a rhododendron decorum tissue-culture quick propagation method, and particularly relates to a quick propagation method which adopts a tissue culture technique to carry out the callus induction to form caespitose shoots which are differentiated to a complete plant. The rhododendron decorum tissue-culture quick propagation method comprises the following steps: a. preparation of explants; b. establishment of aseptic culture system; c. induction and propagation culture of caespitose shoots; d. elongation of the caespitose shoots and sound seedling culture; e. rooting induction culture of test-tube plantlets; and then after being cut into a single plant, each caespitose shoot with the height of 2cm to 3cm is inoculated onto rooting culture medium, the culture condition is that the culture temperature is 25 DEG C plus or minus 1, illumination time is 12h.d-1, and the illumination strength is 40mol.m-2.s-1 to 60 mol.m-2.s-1; the caespitoes shoot begins to rooting after 50 days, the rooting rate is 60 percent to 65 percent, the height of the seedling can reach 4cm to 5cm after 70 days, the quantity of the root can reach 2 to 3, and the complete plant is transplanted into substrate which is the mixture of yellow sand, perlite and humus soil to be processed in domestication culture. The rhododendron decorum tissue-culture quick propagation method has the advantages that the culture period is shortened, the method is free from being influenced by the season, the survival rate is high, the yield is high and the like.

The invention provides a sterile grafting method of peanut tissue culture seedlings. The method comprises the following steps of: taking sterilely-germinated peanut seedlings as parental stocks; cutting off cotyledons and parts above the cotyledons; longitudinally cutting the middle of lower plumular axes into notches; taking the peanut tissue culture seedlings as scions; cutting the lower end ofeach scion to form a V-shaped wound with the lengths of two inclined planes of 0.2-0.4 cm; inserting the scions into the middle parts of the notches to form a layer tight contact by the parental stocks and the scions; paving a grafting opening by using an opening sealing membrane to avoid losing water and accelerate healing; placing grafted seedlings into a solid culture medium to be sterilely cultured for 3-4 days; then transplanting the grafted seedlings into a sterilized seedling growing base material to be domesticated in a domestication room; then transplanting the seedlings into fields and managing the seedlings according to a field planting method. In the method provided by the invention, the parental stocks are grafted on the lower plumular axes with the notches below the cotyledons; the grafting operation is simple, the trouble of the need of usually removing lateral buds on the parental stocks is saved after the seedlings are transplanted to the fields, and the nutrition is sufficiently supplied to the scions; the tissue culture seedlings can be used for grafting when the lengths of the tissue culture seedlings are not more than 1 cm and the application range is wide; and the healing of the grafting wound can be accelerated and the dissemination of miscellaneous bacteria is prevented.

The invention provides a method for cultivating healthy grafting seedlings of cucumbers. Turf, vermiculite and pearlite are mixed according to the volume ratio of 6:3:1 to prepare seedling cultivating substrates, teaselgourd in Africa is selected as stock, and the operation of raising seedlings by grafting in plug trays is performed; the stock is sown 5-7 days earlier than scions, and the stock is sown in 72-hole plug trays; the scions are sown in flat trays; 5-8 ml of a potassium phosphite aqueous solution with the concentration being 50 mg.kg-1 is applied to each tomato seedling from the time when the stock buds to the time when cotyledons are expanded; grafting is performed when the core of a first main leaf of the stock is exposed, the steam thickness is 2.5-3 mm, seed leaves of the scions become green, and the stem thickness is 1.5-2 mm; 8-10 ml of a prohexadione-calcium aqueous solution with the concentration being 20 mg.kg-1 is applied to each cucumber seedling when the length of a second main leaf is 1 cm after grafting; 10-12 ml of a Dufulin aqueous solution with the concentration being 100 mg.kg-1 is applied to each cucumber seedling when the length of a third main leaf is 1 cm after grafting. The grafting seedlings cultivated through the method have a remarkable preventing and treating effect on the root-knot nematode disease and virus diseases in the field.

The invention relates to a directional seedling picking device in a grafting machine and aims to provide a device which can efficiently cut and pick grafted seedlings, and the cotyledon spreading directions of the obtained grafted seedlings are uniform and ordered. The technical scheme is that: the directional seedling picking device for a melon grafted seedling cotyledon is provided with a machine frame, wherein the machine frame is provided with a plurality of seedling picking units which are driven by a movement mechanism and are arranged straightly in parallel; each seedling picking unit comprises a cutting component driven by an air cylinder and a cotyledon direction regulating part; in the cutting component, two semiorbicular shearing blades are fixed on a left oscillating bar and a right oscillating bar respectively; the left oscillating bar and the right oscillating bar are rotatably hinged on a unit base respectively and are hinged with one end of a connecting rod respectively; the other end of the connecting rod is hinged at the top end of a piston rod of the air cylinder; the cotyledon direction regulating part is provided with two guide sleeves of which both bottom ends are vertical respectively and the concave surfaces are oppositely fixed on the upper semi-cylindrical surfaces of the left oscillating bar and the right oscillating bar; and the top edges of both guide sleeves are mutually symmetrical beveled edges or peak-shaped edges respectively.

The invention relates to a torreya grandis seed after-ripening treatment method for promoting embryo development, used for gymnosperm taxaceae torreya.. Pearlite or river sand is soaked by clear water under the condition that no water is dropped when the pearlite or the river sand is held by hands and a soaked pearlite or river sand layer is paved at the bottom of a container, then torreya grandis seeds are placed in a layer way, and the soaked pearlite or river sand layer is covered on the torreya grandis seeds, and then other seeds are placed again, and then the soaked pearlite or river sand layer is covered on the seeds by parity of reasoning until the upper part of the container is covered by the pearlite or river sand layer; then poikilothermic treatment is carried out for 90-100 days; inspection is carried out and the pearlite or river sand is maintained to be moist, and no water is accumulated; after the seeds are subjected to after-ripening treatment for 90 days by adopting the method, not only reaches embryo development rate up to more than 95%, and dissection shows that torreya grandis grow to a fresh green mature embryo with the length of being about 2cm from a flavescent young embryo with the length of being about 0.001cm and the size same as that of a needle point, and the mature embryo almost penetrates trough the whole torreya grandis seed; and secondly, the torreya grandis seed after-ripening treatment method disclosed by the invention is applicable to sowing, raising seedling and tissue culture of the mature embryo, cotyledons, embryo, embryonic axis and endosperm of the torreya grandis and is also applicable to all torreya plants in the world.