26 results about "Cotyledon" patented technology

The application belongs to the field of in vitro rapid propagation of Chinese medicine seedling, and particularly relates to a high-efficiency liquid suspension culture method of pinellia ternate, which comprises obtaining of pinellia ternate explants under sterile conditions, induction of embryogenic callus, liquid suspension culture, somatic embryo development and maturation, somatic embryo germination and transplanting of regenerated seedlings. By using the method, the induction rate of pinellia ternate embryogenic callus can reach 81.6%, stable mature cotyledon embryos can be obtained, and the embryos can be regenerated into complete plants, a high-efficiency and stable regeneration system is successfully established, favorable conditions are created for industrial production of pinellia ternate fine seedlings, and a good foundation is laid for genetic transformation of pinellia ternate.

This invention discloses a method, system, and device for branching the gene sequence of influenza A virus, relating to the field of bioinformatics. The method includes the following steps: reading a user-input phylogenetic tree structure file and a Fasta file; dividing the phylogenetic tree into multiple branches based on a set first custom parameter and the gene sequence data of each cotyledon node; verifying the relationship between branches based on a set second custom parameter, and merging branches whose genetic distance is within a set threshold; verifying the merged branches; and if there are unassigned cotyledon nodes, assigning them to the branch with the closest genetic distance that satisfies the custom parameter, thereby obtaining the final branching result of the influenza A virus gene sequence. The method employed in this invention enables targeted phylogenetic branching based on the characteristics of influenza A virus, improving the accuracy of the branching results.

The application discloses a kind of similar bundle-like arabino-galactoside protein coding gene GhFLA2 In the application of regulating cotton seed vigor, the application obtains several overexpression materials, RNA interference materials and gene knockout materials by transgenic technology GhFLA2 It is found that overexpression GhFLA2 Can promote radicle elongation during seed germination and cotyledon unfolding after germination, GhFLA2 After the expression amount of the application is reduced or knocked out, radicle elongation and cotyledon development are inhibited.The application provides a new gene target for cotton seed vigor improvement.

This invention belongs to the field of mung bean sprout growth analysis technology, specifically relating to a method and system for image recognition of mung bean sprout growth status. This invention divides the mung bean sprout into stem axis, cotyledon, and root tip regions, allowing for independent modeling of the growth characteristics of different functional regions. This improves the ability to analyze complex growth morphologies. By extracting multi-dimensional dynamic parameters such as length, diameter, angle, color, position, and density, a comprehensive quantitative description of the mung bean sprout growth process is achieved. This overcomes the limitations of traditional methods that rely solely on static features. By performing spatiotemporal unified processing on multi-time-series image data, and combining growth trajectory construction, structural feature analysis, and dynamic parameter modeling, standard growth reference information is introduced for comparative analysis. This enables continuous identification and anomaly judgment of mung bean sprout growth status, effectively improving the accuracy, stability, and interpretability of the identification.

The present application relates to the field of plant growth regulator technology, and in particular, to a phenylurea compound, a method for preparing same, and use thereof, and a composition for promoting crop cell division and a method for using same. The phenylurea compound provided herein has cytokinin-like biological activity and can effectively promote the germination of crop seeds and the expansion of cotyledons, increase the chlorophyll content, and retain and enlarge the fruits, featuring a stable action effect, safety to crops, and the like. Experimental results show that the phenylurea compound of the present application can effectively promote the germination of rice and peanut seeds, expansion of radish and cucumber cotyledons, leaf pigment retention and yield increase of peanuts, fruit set and retention of zucchini, and enlargement and sweetness enhancement of grape fruits, etc., exhibiting cytokinin-like effects.

In a plant cultivation method according to the present invention, in the second step which corresponds to a period from cotyledon opening to inflorescence development, PPFD-VL is set to be 29 (μmol / m2·s) or more and 120 (μmol / m2·s) or less, PPFD-B is set to be 5 (μmol / m2·s) or more and 20 (μmol / m2·s) or less, PPFD-G is set to be 10 (μmol / m2·s) or more and 50 (μmol / m2·s) or less, PPFD-R is set to be 14 (μmol / m2·s) or more and 50 (μmol / m2·s) or less, and a color temperature of irradiation light is set to be 3000 K or higher and 4800 K or lower.

The application discloses a method for delaying leaf senescence of Brassica chinensis var. rosularis by editing genes SGR1 and SGR2, and belongs to the technical field of crop breeding. The method comprises the following steps: designing specific sgRNA according to the coding region sequence of the stay-green genes SGR1 and SGR2 of the Brassica chinensis var. rosularis, constructing a CRISPR / Cas9 gene editing vector, transforming the Brassica chinensis var. rosularis receptor material by means of Agrobacterium tumefaciens infection of the cotyledon and hypocotyl, obtaining the transformed plant containing the gene editing element through adventitious bud regeneration and screening, and screening the plant with the SGR1 and / or SGR2 gene site-directed mutation through molecular identification and phenotype observation. The application precisely modifies the stay-green genes by the gene editing technology, significantly delays the leaf senescence process of the Brassica chinensis var. rosularis, improves the leaf stay-green property and the preservation period, and keeps excellent agronomic traits, thereby providing a new way for the genetic improvement and preservation technology development of the Brassica chinensis var. rosularis, and having important application value in the fields of vegetable production, preservation and breeding.

The application relates to a method for efficiently propagating plants through long-beaked thick-embryonic cells, and belongs to the technical field of plant tissue culture. The method comprises five steps of embryonic cell induction, proliferation and maintenance, somatic embryo differentiation and maturation, germination and seedling growth and domestication and transplanting. By optimizing the sucrose concentration in the induction medium to 50 g / L and adopting the technology of combining cell cluster sorting with suspension culture, the induction rate and the proliferation speed of the embryonic cells are significantly improved; by controlling the inoculation density and adding glutamine and abscisic acid in combination during the somatic embryo differentiation stage, about 67 cotyledon embryos can be generated from 200 cell clusters; during the germination stage, by synergistically using gibberellin and Karrikin 1, the synchronous development of roots and stems is promoted, and robust seedlings with multiple true leaves and multiple main roots are obtained. The application first realizes the large-scale propagation of about 6800 somatic embryo seedlings from 1 gram of embryonic cells, and the transplanting survival rate reaches 96.2%, thereby providing an innovative technical scheme for the germplasm preservation and efficient propagation of long-beaked Magnolia officinalis.

The application belongs to the field of biology and modern agricultural technology, and discloses a rooting aid invasion agent, and a formula thereof is as follows: B5 culture medium 3.0-3.5 g / L, sucrose 28-32 g / L, MES 3.8-4.0 g / L, acetyl-syringone 38-42 mg / L, 6-BA 1.5-1.8 mg / L, gibberellin 0.02-0.03 mg / L, and surfactant Silwet L-77 100 ml / L, and pH is 5.4. The application also discloses a soybean hairy root induction and transformation method mediated by Agrobacterium rhizogenes by using the rooting aid invasion agent, and the method comprises the following steps: disinfection and germination of soybean seeds; resuspension of Agrobacterium rhizogenes with a target expression plasmid in the rooting aid invasion agent to obtain an Agrobacterium rhizogenes aid invasion liquid; infection; co-culture; and screening of positive roots.

The application provides a method for improving drought resistance of macadamia rootstock by overexpressing MiNAC2a gene, and belongs to the technical field of planting, and the method comprises the following steps: obtaining cotyledon of aseptic seedling from macadamia seed, inducing callus by using the cotyledon of the aseptic seedling, constructing an overexpression vector according to the obtained MiNAC2a gene sequence, transferring the vector into agrobacterium, mediating the agrobacterium to transfer into macadamia callus by injection method, culturing positive callus to grow adventitious buds and adventitious roots, extracting DNA of the seedling to verify whether the positive plant is true or not by detecting bands, hardening the seedling, transplanting the seedling to a field, obtaining transgenic drought-resistant rootstock resources, strengthening the root system of the transgenic seedling, and improving the drought resistance of the rootstock. The technology uses transgenic technology to cross boundaries, break through reproductive isolation, and broaden the gene resource library, and provides a train of thought for precisely introducing key drought-resistant genes in bacteria, fungi and even other drought-resistant plants into macadamia trees.

The present application relates to the technical field of breeding and seedling raising of forest trees, and discloses a method for realizing in vitro regeneration of Rosa roxburghii by hypocotyl culture, which comprises the following steps: taking seedling germinated from cold storage of Rosa roxburghii seeds as material, removing cotyledon and root, cutting hypocotyl segments with a length of 0.6-1.5 cm as explants, and sterilizing the explants; inoculating the sterilized explants into primary induction medium, and carrying out dark culture until callus is generated and primary adventitious buds are induced; inoculating the cultured callus into differentiation medium, and carrying out dark culture until secondary adventitious buds are regenerated; inoculating the obtained secondary adventitious buds with a height of 1.5-2.5 cm into rooting medium until rooted plants are obtained; and hardening the obtained rooted plants, and then transplanting them into mixed substrate for culture.

This invention relates to the field of agricultural technology, specifically to a mutant Arabidopsis protein and gene that promotes chloroplast development, and their applications. The mutant Arabidopsis protein is the AtMurE protein in wild-type Arabidopsis Sig6 factor-deficient mutants, with mutations occurring at positions 89, 97, 106, 125, 126, 130, or 140. The mutant protein or gene provided by this invention, when introduced into wild-type Arabidopsis Sig6 factor-deficient mutants, can restore chlorophyll content and resolve the cotyledon yellowing problem in wild-type Arabidopsis Sig6 factor-deficient mutants.

The application relates to a European spruce mature cotyledon embryo phenotype intelligent detection method and system and a storage medium, and relates to the cross technical field of forest tree breeding and computer vision. The detection method in the application first constructs a mobile phone image data set of European spruce somatic embryo differentiation and performs boundary box labeling; secondly, a MultiSeamHead module is introduced based on a YOLOv11n framework, the detection capability for small targets, dense occlusion and complex backgrounds is enhanced through multi-scale feature fusion and a spatial attention mechanism; then, the improved detection model is trained by using the labeled data set; finally, a visual interactive platform is constructed based on PySide6, the trained model is integrated, and the functions of image import, automatic detection, result visualization and data export are realized. Experiments show that the precision of the improved model reaches 0.861, the recall rate reaches 0.863, and the mAP@50 reaches 0.927, which is significantly improved compared with the original model, efficient, accurate and high-throughput mature cotyledon embryo phenotype automatic detection is realized, and the technical application threshold is reduced.

This invention discloses a SNP marker and detection primer pair associated with the adventitious root primordium trait of *Senecio scandens* with stem nodules, and its application, belonging to the field of molecular biology technology. Based on SNP marker technology, this invention develops an SNP marker and detection primer pair linked to the adventitious root primordium trait of *Senecio scandens* with stem nodules. This invention involves phenotypic identification of mutants induced by EMS mutagenesis in *Senecio scandens* with stem nodules, followed by isolation, purification, population construction, and genetic analysis combined with Mutmap. + The SNP marker associated with adventitious root primordia is located at 10,433,968 bp on chromosome 4 of *Senecio scandens*, involving a C / T mutation. When the genotype is T, *Senecio scandens* lacks adventitious root primordia. This invention's SNP molecular marker can screen F2 populations at the early cotyledon stage, identifying mutants and wild types without phenotypic differences, thus improving screening efficiency, shortening the breeding cycle, and demonstrating value in marker-assisted breeding.

The application discloses application of a soybean GmSTM17 gene in regulation of soybean cotyledon node organogenesis and belongs to the technical field of biological breeding. In order to improve the regeneration potential in the process of soybean cotyledon node organogenesis, the application provides a method for regulating soybean cotyledon node organogenesis, which comprises regulating expression of a GmSTM17 protein coding gene in a receptor soybean to regulate cotyledon node organogenesis of the receptor soybean, and the amino acid sequence of the GmSTM17 protein is sequence 3. The application also provides application of the GmSTM17 protein in regulation of the regeneration capacity of plant tissues. It is disclosed that the GmSTM17 gene has an inhibiting effect on the growth and development of the clump-bud meristem cells, thereby negatively feeding back regulating the regeneration potential, regeneration efficiency and regeneration rate of the soybean cotyledon node explants. The application provides a theoretical basis for further analyzing the soybean regeneration mechanism and cultivating a soybean variety with high regeneration capacity.

This application discloses a method for cultivating cotton in saline-alkali land to reduce salinity and alkalinity, relating to the field of cotton planting technology. The method includes the following steps: deep plowing and applying base fertilizer to the cotton field, followed by irrigation to create a soil-free environment for sowing; sowing cotton using direct seeding with plastic film mulching and machine-harvested cotton planting, controlling the sowing depth to 2.5–3.5 cm, the soil covering width to 5–7 cm, the soil covering thickness to 0.5–1 cm, the 1-seed rate to ≥95%, and the empty seed rate to ≤3%; post-sowing management during the seedling stage, including timely cultivation, and foliar fertilization at the cotyledon to 2-leaf stage, 3–4-leaf stage, and 4-leaf stage; water and fertilizer management during the budding stage, including cultivation and foliar fertilization; drip irrigation and fertilization during the boll-forming stage, including timely topping, and additional fertilization to regulate plant shape, with separate fertilization for premature aging and excessive vegetative growth; and pest and weed control during the sowing period. The cultivation method described in this application is applicable to saline-alkali cotton fields in Xinjiang and is an effective means to increase cotton yield and improve soil conditions.

The application relates to a genetically engineered body, and particularly discloses a perilla frutescens PfCHLH gene and a virus-induced gene silencing system, a construction method and application of the gene silencing system. PfCHLH The nucleotide sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. PfCHLH The gene silencing fragment is cloned into a virus carrier pTRV2 to obtain a pTRV2- PfCHLH silencing carrier, and the pTRV2 carrier and the pTRV1 carrier are respectively transformed into agrobacterium GV3101 to prepare infection bacterial liquid; the infection bacterial liquid is mixed according to a volume ratio of 1:1 and then injected into perilla frutescens cotyledons to induce gene silencing. PfCHLH qRT-PCR verification shows that the expression amount is significantly down-regulated and a stable yellowing phenotype is generated; preferably, the OD 600 of the infection bacterial liquid is 0.6, the silencing phenotype is more significant, and the consistency is better. The perilla frutescens VIGS system established in the application is simple in operation, short in cycle, and high in efficiency, and can be used for rapid identification of perilla frutescens gene functions and verification and application of key genes in related metabolic pathways.

The application discloses a method for promoting adventitious shoot regeneration of sunflower in vitro, and belongs to the technical field of plant tissue culture. The method comprises the following steps: taking sunflower 'YK86' sterile seedling cotyledon as an explant, inoculating the explant into a callus induction medium containing 0.01 nM exogenous HaREF1 small peptide and a bud differentiation medium in sequence after pretreatment, effectively promoting sunflower callus induction and adventitious shoot differentiation and regeneration, reducing the browning rate, and successfully inducing adventitious shoots. Through screening of the genotype and explant of sunflower in vitro regeneration, in combination with the synergistic effect of screening and adding different concentrations of HaREF1 to the callus induction medium and the bud differentiation medium, the application effectively promotes the differentiation and regeneration of sunflower adventitious shoots in vitro, solves the problems of difficult sunflower in vitro regeneration, low regeneration efficiency and instability, and provides technical support for sunflower genetic transformation, gene function verification and variety improvement.

The application discloses a pepper cotyledon color molecular marker and application thereof in detecting pepper cotyledon color. The pepper cotyledon color molecular marker disclosed by the application is a nucleotide corresponding to the 123th nucleotide of SEQ ID No. 1 in the sequence listing in the pepper genome, which is A or G. Experiments prove that in a pepper population of 548 strains, the cotyledon of 147 homozygous peppers with the nucleotide corresponding to the 123th nucleotide of SEQ ID No. 1 in the sequence listing in the genome DNA being A is purple, the cotyledon of 277 heterozygous peppers with the nucleotide corresponding to the 123th nucleotide of SEQ ID No. 1 in the sequence listing being A and G is purple, and the cotyledon of 124 homozygous peppers with the nucleotide corresponding to the 123th nucleotide of SEQ ID No. 1 in the sequence listing being G is green. It is shown that the pepper cotyledon color molecular marker of the application can be used for identifying pepper cotyledon color and hybrid breeding of peppers.

The present application belongs to the field of plant tissue culture and bioengineering, and particularly relates to a method for genetic transformation of pepper mediated by Agrobacterium and application thereof. The method comprises the following steps: S1, using cotyledon and hypocotyl stem segment of pepper seedling as explants; S2, directly using Agrobacterium liquid with OD600 of 0.3-0.6 to infect the explants without pre-culture; S3, transferring the explants to callus induction medium after co-culture; S4, transferring the explants to bud emergence medium after green bud spots appear on the explants; S5, cutting the bud and transferring it to rooting medium after the regenerated bud elongates; and S6, performing transgenic detection on the transgenic plants after rooting. The present application provides a method for genetic transformation of pepper mediated by Agrobacterium, and the effective transformation efficiency reaches more than 5%, which breaks through an important bottleneck of genetic transformation of pepper. Through the transformation system, gene editing of pepper can be performed to obtain edited plants, which has great application value.

This invention provides an application and method for the sodium-calcium transporter SlNCL2.2 in tomato salt stress. The invention involves constructing an SlNCL2.2 gene overexpression vector [pBWA(V)HS-TS-21 OE-N2.2] and an SlNCL2.2 gene knockout editing vector [zmpl-LACas-2]. These vectors are then introduced into Agrobacterium-mediated transformation into Agrobacterium strain GV3101. Tomato cotyledons are used as explants to transform the GV3101 strain into salt-tolerant tomato TS-21, thereby obtaining SlNCL2.2 transgenic overexpression and gene knockout materials. The role of the sodium-calcium transporter SlNCL2.2 in tomato salt stress is comprehensively analyzed by measuring phenotypic indicators such as plant height and root length, and physiological and biochemical indicators such as proline, POD, and ion content. Under salt stress, overexpression of the SlNCL2.2 gene significantly promoted tomato growth and enhanced the tolerance of tomato seedlings to salt stress compared with wild-type TS-21. Conversely, under salt stress, knocking out the SlNCL2.2 gene led to tomatoes being more sensitive to salt stress and having reduced salt tolerance compared with wild-type TS-21.

A method for analyzing a population of seeds comprising creating an orifice in each seed of the population of seeds, thereby exposing a portion of a cotyledon or endosperm of the seed; removing debris from the orifice; applying a first solution into contact with the orifice and onto the exposed portion of the cotyledon or endosperm, thereby forming a seed soak solution from the seed; and removing the seed soak solution while preserving germination viability of the seed. Applying a solution into the exposed orifice of the seed creates a seed soak solution in situ from which a biological molecule representative of an attribute of the individual seed, such as DNA indicative of a trait, can be isolated and analyzed.

A sowing and seedling cultivation method for Shaniodendron subaequale is provided, which relates to the field of plant seedling technologies. The method includes: collecting seeds from mid-September to early-October; removing impurities and air-drying the seeds until a seed moisture content is 8%-10%; performing sand storage on the seeds for overwintering by dynamically controlling humidity and using a sand-seed-sand layering method, and performing turning and disinfection on the seeds during the sand storage; sowing the seeds on a seedbed when a seed germination rate reaches 20%-30% in a following year, and covering the seeds with fine soil and dry grass; and removing the dry grass gradually when cotyledons of 20%-30% of seedlings are unfolded, and performing conventional seedling management. The method achieves a seedling emergence rate of 80%-85% and a seedling survival rate of 95%-98% for Shaniodendron subaequale. The cultivated seedlings are robust and exhibit strong adaptability to the environment.