243 results about "Cotyledon" patented technology

The invention relates to the technical field of plant tissue culture, in particular to a Rhododendron pulchrum tissue culture method based on callus regeneration. According to the method, the tender cotyledons of aseptic seedlings are taken as explants, and an efficient Rhododendron pulchrum tissue culture and rapid propagation technical system is established through induction of calluses, subculture multiplication of the calluses, differentiation of adventitious buds, bud strengthening and rooting culture, so that complete tissue culture progeny plants are formed. The induction rate of the callus tissue of the system is 100.0%, the proliferation multiple of the callus tissue in a subculture cycle reaches 5.2 times, 28.5 adventitious buds can be differentiated from a callus tissue block with the size of about 0.5 mm * 0.5 mm * 0.5 mm, the rooting rate reaches 95.8%, the system is not limited by factors such as seasons, climate and regions, and efficient and rapid propagation can be achieved all the year round. Meanwhile, the establishment of the system also lays a technical foundation for genetic transformation improvement, mutant screening breeding and the like of the Rhododendron pulchrum based on the callus.

The invention provides a crop population three-dimensional reconstruction and organ phenotypic character analysis method, and relates to the field of three-dimensional reconstruction, and the method comprises the steps: collecting cotton envelope image data, carrying out the data preprocessing, and carrying out the motion recovery processing and Gaussian sputtering of the cotton envelope image data, the method comprises the following steps of: preprocessing a group cotton point cloud, removing a ground point cloud, obtaining a complete cotton group point cloud model, segmenting a cotton individual point cloud model and a corresponding organ point cloud model from the complete cotton group point cloud model, automatically analyzing cotton key phenotypic characters, and performing correlation analysis. According to the method, a cotton original habitat three-dimensional point cloud model is constructed based on mobile visual equipment, and key characters such as cotyledon node height, plant height and leaf area can be automatically analyzed, so that the problems that phenotypic character extraction in the prior art is high in cost, low in flux, easy to make mistakes, inconsistent in standard, generally destructive, labor-consuming, time-consuming and the like are solved.

A method for applying at least one spray product to agricultural land having crop plants and weeds. The crop plants and weeds can be assigned to a cotyledon class of monocotyledons or to a cotyledon class of dicotyledons. The at least one spray product is applied to the acquired field section of the agricultural land by means of at least one spray nozzle unit of an agricultural spraying device depending on the cotyledon class of the crop plants and a first ascertained weed identification number and / or a second ascertained weed identification number.

The invention relates to a method for producing notoginsenoside and ginsenoside by utilizing panax notoginseng cotyledon embryos, and belongs to the technical field of plant adventitious root tissue culture. The method comprises the following steps: inducing embryogenic calluses by utilizing immature zygotic embryos of panax notoginseng; proliferating the panax notoginseng embryogenic callus on a plate culture medium; s2, putting the pseudo-ginseng embryogenic callus obtained in the step S2 into a liquid culture medium, carrying out cell dispersion, and sieving by using a sieve with the aperture of 24-30 meshes; a mechanical stirring type bioreactor is filled with a liquid culture medium, and the globular embryo and the heart-shaped embryo are placed in the mechanical stirring type bioreactor to be cultured; s3, recovering the filtrate in the step S3, filtering by using a screen with the aperture of 30-80 meshes and 80-150 meshes to obtain uniform panax notoginseng embryogenic cells, and differentiating the panax notoginseng embryogenic cells in a triangular flask for the step S4; and drying the cotyledon embryo obtained in the step S4 at 45 DEG C, and grinding into powder. According to the method, large-scale generation of cotyledon embryos can be completed only in 4-6 weeks, the production process is remarkably accelerated, the method is suitable for industrial batch production requirements, and the production efficiency is high.

The invention belongs to the technical field of grafting and seedling raising, and discloses a method for grafting and seedling raising by reserving a first true leaf of a pumpkin rootstock, which comprises the following steps: selecting the pumpkin rootstock; scion and stock seedling raising; reserving true leaves for grafting; grafted seedling healing period management; the specific operation of keeping the true leaves for attaching grafting is as follows: the true leaves and one of the cotyledons are separated to one side by the thumb and the index finger of the left hand, then a grafting blade disinfected by alcohol is used for beveling along the middle of the true leaves and a growing point, a notch is preferably used for simultaneously removing one of the cotyledons and the growing point, the other cotyledon is kept, and the notch is not too long and is about 1cm preferably; the scion is beveled by 30 degrees at the position 1-2 cm below the cotyledon base, it is guaranteed that the cut surface is smooth, and the length of the cut is about 1 cm. Grafted seedlings obtained through the method have higher root system related physiological indexes, grafting survival rate, SPAD value, plant height, stem diameter and leaf number, and healthy growth and strong seedling indexes of the seedlings are integrally improved.

The invention discloses an olive tetraploid induction method, and belongs to the field of olive breeding. The induction method provided by the invention comprises the step of treating growing points among cotyledons of the olive sterile tissue culture seedlings by using an induction solution, wherein the induction solution is a mixed solution which is prepared by using a 0.2% dimethyl sulfoxide (DMSO) aqueous solution as a solvent and contains 0.1%-0.5% of colchicine and 0.02%-0.04% of oryzalin. According to the method, the production of the olive tetraploid can be effectively induced, and the method has relatively low material death rate and relatively high material ploidy variation rate.

The present disclosure provides methods of gene transformation of sunflower based on Agrobacterium-mediated gene delivery to meristem cells of fresh imbibed / germinated sunflower seeds. One embodiment of the present disclosure is a method of directly transforming a sunflower from a mature seed with a heterologous polynucleotide, comprising: generating an explant from a germinated sunflower seed by removing cotyledons and primary leaves having intact meristem and tender shoot radicles; contacting the sunflower explant comprising the cotyledon with Agrobacterium comprising a heterologous polynucleotide in an infection medium supplemented with a plant hormone using vacuum and ultrasound treatment; co-culturing the explant from a germinating sunflower plant in a culture medium lacking the plant hormone in the presence of the Agrobacterium genus; culturing the mature seed explant in a regeneration medium comprising a selective agent; and culturing the seed with the regenerated tender shoots and with the secondary roots in a culture medium lacking the selective agent.

The invention discloses an application of a gene GmAGO5 of a soybean ARGONAUTE family. The gene GmAGO5 of the soybean ARGONAUTE family is shown The nucleotide sequence of the soybean GmAGO5 protein coding gene GmAGO5 is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the soybean GmAGO5 protein coding gene The constructed plant gene editing knockout vector pSCM-GmAGO5 is transferred into an acceptor material Tianlong No.1 by using a cotyledonary node transformation method. The GmAGO5 gene is knocked out, so that the plant height of the gene edited soybean is reduced, the branch included angle is reduced, and the protein content of the soybean seeds is remarkably increased. Therefore, the soybean GmAGO5 protein coding gene GmAGO5 disclosed by the invention can be applied to reducing soybean plant height, reducing branch included angle and improving the protein content of soybean seeds through a genetic engineering means.

The invention belongs to the technical field of gene engineering and salt-tolerant plant cultivation, and particularly relates to a wild soybean salt-tolerant gene and application thereof. The gene GsWRKY53 responding to salt stress is identified by performing expression analysis on the gene of the wild soybean under salt treatment, the salt-tolerant gene GsWRKY53 in the wild soybean is transferred into a soybean cotyledon through an agrobacterium transformation method for the first time, and the gene function of the gene can be quickly and accurately verified by verifying the influence of the salt-tolerant gene GsWRKY53 on soybean hairy roots. Experiments prove that the fresh weight, the dry weight and the number of hairy roots of soybeans can be reduced by overexpression of the wild soybean salt-tolerant gene GsWRKY53, which indicates that the GsWRKY53 plays a negative regulation role in the salt tolerance of the soybeans. According to the technical scheme, a foundation is provided for screening and verifying the salt-tolerant functional gene and further screening or cultivating salt-tolerant plant varieties, especially salt-tolerant soybean varieties, so that the method has a good practical application value.

The invention belongs to the technical field of plant monitoring, and particularly relates to a nondestructive monitoring method and system for judging the healing state of a muskmelon grafted seedling by using a wearable sensor, and the method comprises the steps: preparing a pumpkin rootstock and a muskmelon scion for grafting; grafting when the first true leaf of the pumpkin rootstock is completely flattened and the first true leaf of the scion is half-expanded; a sensor is attached to the cotyledon of the scion to continuously detect the growth state of the grafted seedling; impedance signals of the sensor are measured through an instrument, and an impedance change curve 10 days after grafting is drawn to evaluate the grafting healing state. According to the method, the physiological activity state of the grafted seedling can be directly monitored, so that whether the healing state is good or not can be judged in advance.

The invention belongs to the technical field of biology, particularly relates to the technical field of plant genetic engineering, and more particularly relates to application of an MYC gene in regulation and control of chloroplast development and composite stress resistance. Specifically, the invention innovatively discovers that the combined action of warm and JA can significantly induce seedling cotyledon chloroplast dysplasia, and deletion / reduction of MYC gene expression can significantly inhibit chloroplast dysplasia of plants under the combined action of warm and JA. The discovery provides guidance for remarkably improving plant seedling chloroplast development in a complex dynamic environment and improving the seedling survival rate and photosynthetic productivity, can promote optimization of a chloroplast artificial regulation and control technology, and realizes directional design of efficient photosynthetic organs in facility agriculture and an artificial photosynthetic system.

The present invention relates to the technical field of molecular markers, in particular to a molecular marker associated with cold resistance of corn and the use thereof. The molecular marker associated with the cold resistance of corn is ZmTIP4;3, and is obtained by amplification using primers shown as SEQ ID NO. 1 and SEQ ID NO. 2. Since the cold resistance of corn is a quantitative trait, phenotype analysis is time-consuming and labor-consuming. The molecular marker can be applied to cold-resistant corn breeding, and identification can be achieved in the corn seed stage or the early stage of cotyledon emergence, which saves time and is accurate, thus accelerating the process of breeding cold-resistant corn varieties.

The invention discloses a method for green and efficient extraction of functional components in spina date seeds. The method comprises the following steps: crushing spina date seeds, removing seed coats and endosperm, deoiling cotyledons with petroleum ether, performing low-temperature water extraction on peeled and deoiled spina date seed powder, filtering and clarifying extract liquid with a hollow fiber membrane or a ceramic membrane, performing reverse osmosis concentration to remove most of water, and drying concentrated liquid to obtain the spina date seed extract highly enriched with functional components. In the extraction process, petroleum ether is used in the deoiling part, only water is used in the other steps, no solvent with obvious toxicity and pollution is used in the whole extraction method, the method has the advantages of safety, environmental protection, simple process, low cost, good extraction effect and the like, and the obtained extract is green and safe, can be directly used in the fields of medicines, foods and the like, and has wide application prospects. And by-products such as seed coats and endosperm of spina date seeds, extracted spina date seed oil and spina date seed starch and protein settled at the bottom after extraction can be further utilized and developed, so that more additional values are created for enterprises.

The invention discloses an application of RsNRAMP5 heavy metal transporter protein in cadmium absorption and active oxygen accumulation of radish, which comprises the following steps: (1) cloning RsNRAMP5 by using radish cDNA as a template, and the cDNA sequence of the RsNRAMP5 is as shown in SEQ ID NO.1; (2) the increase of Cd treatment time (6h, 12h, 24h) on the expression level of the RsNRAMP5 gene is obviously up-regulated; (3) overexpression of RsNRAMP5 significantly increases the absorption of Cd < 2 + > in yeast cells; (3) the activity of the RsNRAMP5 promoter in the radish cotyledons can be obviously induced through treatment of 100 [mu] M Cd; and (4) instantaneously transforming the radish cotyledons by using an agrobacterium injection method, analyzing the change of the content of H2O2, O2 <.-> and Cd in the radish cotyledons under the treatment of CdCl2 and the inhibition expression of RNAi, and discovering that the overexpressed RsNRAMP5 promotes the absorption of Cd and the accumulation of active oxygen in the radish cotyledons. The invention provides a theoretical basis for breeding low Cd accumulation radish germplasm by using a genetic engineering technology in future, and has a very high application value.

The invention provides a tissue culture method for seedlings of apple trees, and belongs to the technical field of apple tissue culture breeding, the tissue culture method comprises the following steps: 1) disinfecting seeds of apple trees, removing seed coats, inoculating embryos after cotyledons are cut into a germination culture medium for germination, and obtaining tissue culture seedlings; and (2) cutting adventitious buds from the tissue culture seedlings, inoculating the adventitious buds into a proliferation culture medium, and carrying out proliferation culture to obtain seedlings. According to the tissue culture method, the embryos are used as explants, the embryos have better differentiation and propagation capacity, the regeneration capacity of the obtained tissue culture seedlings is higher than that of the tissue culture seedlings obtained by using other materials as explants, and the propagation coefficient is high.

The invention relates to the technical field of plant genetic engineering, and particularly discloses a construction method of a VIGS silencing system for agastache rugosus gene function research, which comprises the following steps: constructing a TRV2-PDS or TRV2-CHLH visual vector by taking indicator genes PDS and CHLH for chlorophyll synthesis as reporter genes; the method comprises the following steps: transforming a TRV2-PDS or TRV2-CHLH visual vector into agrobacterium, and performing injection infection on cotyledons of a target plant to construct a VIGS silencing system. According to the invention, cloning, amplification and vector construction are carried out on the isopuleone isomerase gene (ArIPI gene) in the agastache rugosus, and the function of the ArIPI gene is verified in a plant body, so that a scientific basis is provided for improving and cultivating the agastache rugosus and constructing genetic engineering.

The invention relates to an agrobacterium tumefaciens-mediated cork tree genetic transformation method. The method comprises the following steps: preparing an infection solution from an agrobacterium solution; the method comprises the following steps: taking in-vitro cotyledons or stems as explants after cork tree seeds germinate into seedlings, infecting the cotyledons or stems of the cork tree by adopting an infection solution, and carrying out co-culture, recovery culture and screening culture after infection is completed, so as to obtain transgenic hairy roots or transgenic calluses. According to the scheme provided by the invention, stable transformation of balsa roots can be realized, verification of functional genes is realized, the functional genes exist in a living body form for a long time, and the genetic transformation efficiency of balsa is improved.

The invention relates to the technical field of plant tissue culture, and particularly discloses an establishment method of a grape seedling hypocotyl induced somatic embryo regeneration system. Comprising the following steps: by taking a secondary embryo obtained from a hypocotyl of a seedless grape embryonic-handle malformed seedling as an explant, inducing in a KBN culture medium to obtain an embryonic callus, a non-embryonic callus and a secondary proembryo mass, inoculating the secondary proembryo mass into an X6 culture medium, and carrying out propagation culture to obtain the secondary proembryo mass with increased quality and a somatic embryo, performing secondary induction on the somatic embryos before the cotyledon stage to establish a somatic embryo maintenance and propagation circulating system, inoculating the somatic embryos into an X3 culture medium to differentiate hypocotyls and radicles, and transferring to a WPM culture medium to culture into seedlings. According to the method disclosed by the invention, the hypocotyl of the seedless grape embryonic malformation seedling is successfully induced to obtain a regeneration system, a secondary proembryonic mass maintenance and propagation circulation system is successfully established, and a new acceptor material can be provided for grape genetic transformation.

The invention relates to the technical field of rubber tree seedling cultivation, and particularly discloses a cultivation method for efficiently obtaining a normal somatic embryo of a rubber tree clone variety, and the cultivation method comprises the following steps: S1, taking an anther newborn somatic cell immature embryo of the rubber tree clone variety, and picking out a cotyledon small embryo; s2, placing the cotyledon small embryos in an improved MS culture medium for maturation culture to obtain mature normal somatic embryos; s3, transferring the mature normal somatic embryo into a somatic embryo germination culture medium for somatic embryo plant induction culture. The method for cultivating the normal somatic embryo of the rubber tree clone variety has better maturation culture efficiency and higher normal somatic embryo and somatic embryo plant obtaining rate, and large-scale propagation can be realized. The maturing rate of cotyledon small embryos reaches up to 82.50% or above, the obtaining rate of normal somatic embryos reaches up to 75.0% or above, and the obtaining rate of somatic embryo plants reaches up to 63.0% or above.

The invention belongs to the field of plant seedling raising, and particularly relates to a Shaniodendron subaequalis sowing and seedling raising method which specifically comprises the following steps: collecting seeds from the middle ten days of September to the first ten days of October; removing impurities from the seeds, and drying in the shade until the water content of the seeds is 8-10%; by dynamically controlling the humidity, the seeds are subjected to sand storage for winter by adopting a sand-seed-sand lamination method, and the seeds are turned over and disinfected for many times during sand storage; sowing the seeds when the germination rate of the seeds in the next year reaches 20%-30%, and covering the seeds with fine soil and hay; hay is gradually removed when 20%-30% of seedling cotyledons are unfolded, and conventional seedling management is carried out. The emergence rate of the Shaniodendron subaequalis cultivated through the method can reach 80%-85%, the survival rate of seedlings can reach 95%-98%, the seedlings are robust and high in environmental adaptability, meanwhile, the method has the advantages of being easy to operate and master and low in cost, and the method has important practical significance on resource protection, scientific research and development and utilization of Shaniodendron subaequalis species.

The invention discloses an agrobacterium rhizogenes mediated sunflower hairy root transformation system, and belongs to the technical field of genetic transformation. The transformation system comprises the following steps: accelerating germination of sunflowers, transferring germinated sunflower seeds into a seedling tray, wrapping with a preservative film for moisturizing, and culturing in an illumination incubator; the method comprises the following steps: culturing an agrobacterium rhizogenes Ar. Qual strain containing a pBI121-GUS (glucuronidase) plasmid; preparing a bacterial liquid; the method comprises the following steps of: beveling sunflower seedlings along a position 3cm below cotyledons, and immersing cuts into a bacterium solution containing pBI121-GUS plasmid agrobacterium rhizogenes Ar.qual; the sunflower seedlings are transferred to a seedling raising plate to be cultured; culturing for 5-6 days, and transferring the seedlings into a filter paper system of a culture dish to promote root growth. According to the agrobacterium rhizogenes-mediated sunflower hairy root transformation system, the genetic transformation efficiency of sunflowers is improved, the transformation period is greatly shortened, and obtained hairy root plants are good in growth consistency, robust in seedlings and capable of being used in molecular breeding research.

Described herein are methods for pre-treating pulses prior to separation of the starch and protein fractions of the pulses by air classification. Specifically, the pre-treatments weaken the structural integrity of pulse cotyledons and physically separate protein and starch inside the cotyledons, which facilitates the separation between protein and starch during subsequent milling and air classification to achieve better purity in both protein-rich and starch-rich fractions. These higher purity fractions have improved functional properties, nutritional properties, and sensory profiles, such as stronger water-holding capacity and oil-absorption capacity, emulsifying capacity and emulsion stability, and foaming properties and foam stability as well as substantially reduced beany flavor and bitter taste.

The present invention provides a special cold-tolerant regulator for cotton seedling stage, its preparation method and application method, belonging to the technical field of crop cultivation. The special cold-tolerant regulator for cotton seedling stage includes chlorophyllin iron and chitosan; the mass ratio of chlorophyllin iron to chitosan is 28-38:500-700. The preparation method of the present invention includes the following steps: (1) Mix chlorophyllin iron with water to obtain solution A; (2) Mix chitosan with glacial acetic acid solution and dissolve to obtain solution B; (3) Add solution A to solution B, and make up the volume with water to obtain the special cold-tolerant regulator for cotton seedling stage. The application method of the present invention is to perform foliar spraying before the occurrence of chilling injury from the cotyledon stage to the five-leaf stage of cotton seedlings. Applying the cold-tolerant regulator prepared by the present invention before the occurrence of chilling injury in the cotton seedling stage can significantly reduce the chilling injury index of cotton seedling leaves and improve the survival rate of cotton seedlings.

The present disclosure provides an application method in rice callus differentiation based on an Oryza sativa Leafy Cotyledon 1 (OsLEC1) gene, including the steps of: selecting guide ribonucleic acid (gRNA) target sites; cloning tandem fragments including gRNA; ligating each gRNA fragment; performing polymerase chain reaction (PCR) amplification on a ligation product; performing enzyme digestion on the purified product and a target vector; transforming the ligated vector; performing Agrobacterium-mediated genetic transformation of rice; and screening and identifying transgenetic plants. In the present disclosure, the differentiation of callus directly affects the emergence efficiency of transgenic plants. The knockout of OsLEC1 can promote the differentiation of rice callus, suggesting that OsLEC1 can serve as an important target gene for improving the transformation efficiency of rice and even gramineous crops. OsLEC1 can be used as a starting point to construct various molecular tools to enhance transformation efficiency.

The invention provides a sesbania cannabina doubling and rapid propagation integrated method, and relates to the technical field of sesbania cannabina breeding, and the method comprises the following steps: rubbing sesbania cannabina seeds to break seed coats, disinfecting the seeds, soaking the sesbania cannabina seeds for hydration, inducing genome doubling by colchicine, and then carrying out rooting culture; after cotyledons are unfolded, water culture seedling hardening is carried out; after compound leaves grow, genome ploidy identification is carried out, and the successfully doubled sesbania can be transplanted. The period of the whole method is 18 days, and the sesbania polyploidy induction rate can reach 50% or above. According to the method, sesbania genome doubling is achieved for the first time, a large number of polyploid plants meeting the breeding requirement can be directly obtained, the seedling raising time can be shortened, the plant survival rate can be increased, the production cost can be reduced, and integration of sesbania genome doubling and plant rapid propagation is achieved.

The invention discloses a method for synergistically improving the lycopene content and the sugar-acid ratio in tomato fruits, belongs to the technical field of biology, and particularly relates to a method for knocking out or silencing an INVINH1 gene to synergistically improve the lycopene content and the sugar-acid ratio in the tomato fruits, and a nucleotide sequence of a protein coding region of the INVINH1 gene is shown as SEQ ID NO.1. The invention further discloses a method for synergistically improving the lycopene content and the sugar-acid ratio in the tomato fruits. A carrier for knocking out or silencing the INVINH1 gene is constructed by utilizing a CRISPR / Cas9 gene editing technology, the carrier is transferred into tomato cotyledons through agrobacterium tumefaciens mediation, INVINH1 gene knockout homozygous strains invinh1-1 and invinh1-2 are obtained through screening by utilizing a tissue culture technology, the lycopene content and saccharic acid in tomato fruits of a mutant plant are remarkably improved compared with those of wild plants, and the gene knockout or silencing method is suitable for industrial production. The invention provides an important gene resource for cultivating tomato varieties for synergistically improving lycopene and sugar-acid ratio.

The present invention relates to a method for producing a composition for promoting hair growth, and more particularly, to a raw material mixing step for mixing ground pine needles, ground pine cones, heartleaf houttuynia herbs, green tea leaves, cotyledons and an ethanol aqueous solution, and to a method for producing a composition for promoting hair growth. A fermentation step: inoculating the mixture cured in the curing step with lactic acid bacteria and fermenting; and mixing the additives, namely mixing the additives containing the hair root cell growth factors. A fermentation product produced by the fermentation step. The composition for promoting hair growth prepared by the method provides nutrition for hair, improves the health of scalp and hair roots, and shows an excellent hair growth effect.

The application relates to the technical field of bean kernel processing, and particularly discloses a bean kernel skin and cotyledon separation device, which comprises a microwave heating device and a mounting frame, the microwave heating device is mounted at the top of the mounting frame, both ends of the microwave heating device are provided with blocking plates, a conveying belt is mounted in the mounting frame, a plurality of placing boxes are mounted on the surface of the conveying belt, each of the plurality of placing boxes is provided with a turnover mechanism, two fixing frames are mounted at the top of the mounting frame, a driving mechanism is arranged at the bottom of each of the two fixing frames, a separation box is arranged at one end of the mounting frame, and an automatic separation mechanism is mounted in the separation box. In the application, the microwave heating device and the separation box are arranged, the skin and the cotyledon of the bean kernel can be automatically separated by using the microwave peeling method, and the efficiency is extremely high; and the turnover mechanism is arranged, the bean kernel can be conveniently turned over, and the separation efficiency of the skin and the cotyledon of the bean kernel is improved.

The invention discloses application of apple MdMYB41L in regulation and control of seed size, and belongs to the technical field of plant genetic engineering. According to the invention, an MdMYB41L gene is cloned from apple GL-3, the CDS nucleotide sequence of the MdMYB41L gene is shown as SEQ ID NO: 1, and a plant overexpression vector is constructed. A transgenic plant is obtained by heterologous transformation into arabidopsis thaliana through an agrobacterium tumefaciens-mediated method. Compared with wild arabidopsis thaliana, the weight, the length, the width and the surface area of the transgenic plant seeds are obviously improved. In addition, the cotyledon area of a transgenic plant is obviously higher than that of a wild type. Therefore, the MYB transcription factor MdMYB41L provides a new thought in the aspects of improving the plant seed size and molecular breeding.

The invention belongs to the technical field of biology, and particularly relates to a soybean hairy root genetic transformation method which comprises the following steps: taking soybean cotyledons as explants, infecting wounds of the explants by utilizing agrobacterium rhizogenes carrying reporter genes, transferring the infected explants into vermiculite, culturing, rooting, and screening positive roots. According to the method disclosed by the invention, a simple and efficient soybean hairy root genetic transformation system is established, tissue culture is not needed, and the problems of long transformation period, high cost and low transformation rate in the prior art are solved.