116 results about "Cotyledon" patented technology

The present disclosure provides methods of gene transformation of sunflower based on Agrobacterium-mediated gene delivery to meristem cells of fresh imbibed / germinated sunflower seeds. One embodiment of the present disclosure is a method of directly transforming a sunflower from a mature seed with a heterologous polynucleotide, comprising: generating an explant from a germinated sunflower seed by removing cotyledons and primary leaves having intact meristem and tender shoot radicles; contacting the sunflower explant comprising the cotyledon with Agrobacterium comprising a heterologous polynucleotide in an infection medium supplemented with a plant hormone using vacuum and ultrasound treatment; co-culturing the explant from a germinating sunflower plant in a culture medium lacking the plant hormone in the presence of the Agrobacterium genus; culturing the mature seed explant in a regeneration medium comprising a selective agent; and culturing the seed with the regenerated tender shoots and with the secondary roots in a culture medium lacking the selective agent.

The invention belongs to the technical field of biology, particularly relates to the technical field of plant genetic engineering, and more particularly relates to application of an MYC gene in regulation and control of chloroplast development and composite stress resistance. Specifically, the invention innovatively discovers that the combined action of warm and JA can significantly induce seedling cotyledon chloroplast dysplasia, and deletion / reduction of MYC gene expression can significantly inhibit chloroplast dysplasia of plants under the combined action of warm and JA. The discovery provides guidance for remarkably improving plant seedling chloroplast development in a complex dynamic environment and improving the seedling survival rate and photosynthetic productivity, can promote optimization of a chloroplast artificial regulation and control technology, and realizes directional design of efficient photosynthetic organs in facility agriculture and an artificial photosynthetic system.

The present disclosure provides an application method in rice callus differentiation based on an Oryza sativa Leafy Cotyledon 1 (OsLEC1) gene, including the steps of: selecting guide ribonucleic acid (gRNA) target sites; cloning tandem fragments including gRNA; ligating each gRNA fragment; performing polymerase chain reaction (PCR) amplification on a ligation product; performing enzyme digestion on the purified product and a target vector; transforming the ligated vector; performing Agrobacterium-mediated genetic transformation of rice; and screening and identifying transgenetic plants. In the present disclosure, the differentiation of callus directly affects the emergence efficiency of transgenic plants. The knockout of OsLEC1 can promote the differentiation of rice callus, suggesting that OsLEC1 can serve as an important target gene for improving the transformation efficiency of rice and even gramineous crops. OsLEC1 can be used as a starting point to construct various molecular tools to enhance transformation efficiency.

The present invention relates to a method for producing a composition for promoting hair growth, and more particularly, to a raw material mixing step for mixing ground pine needles, ground pine cones, heartleaf houttuynia herbs, green tea leaves, cotyledons and an ethanol aqueous solution, and to a method for producing a composition for promoting hair growth. A fermentation step: inoculating the mixture cured in the curing step with lactic acid bacteria and fermenting; and mixing the additives, namely mixing the additives containing the hair root cell growth factors. A fermentation product produced by the fermentation step. The composition for promoting hair growth prepared by the method provides nutrition for hair, improves the health of scalp and hair roots, and shows an excellent hair growth effect.

The application relates to the technical field of bean kernel processing, and particularly discloses a bean kernel skin and cotyledon separation device, which comprises a microwave heating device and a mounting frame, the microwave heating device is mounted at the top of the mounting frame, both ends of the microwave heating device are provided with blocking plates, a conveying belt is mounted in the mounting frame, a plurality of placing boxes are mounted on the surface of the conveying belt, each of the plurality of placing boxes is provided with a turnover mechanism, two fixing frames are mounted at the top of the mounting frame, a driving mechanism is arranged at the bottom of each of the two fixing frames, a separation box is arranged at one end of the mounting frame, and an automatic separation mechanism is mounted in the separation box. In the application, the microwave heating device and the separation box are arranged, the skin and the cotyledon of the bean kernel can be automatically separated by using the microwave peeling method, and the efficiency is extremely high; and the turnover mechanism is arranged, the bean kernel can be conveniently turned over, and the separation efficiency of the skin and the cotyledon of the bean kernel is improved.

The invention discloses application of apple MdMYB41L in regulation and control of seed size, and belongs to the technical field of plant genetic engineering. According to the invention, an MdMYB41L gene is cloned from apple GL-3, the CDS nucleotide sequence of the MdMYB41L gene is shown as SEQ ID NO: 1, and a plant overexpression vector is constructed. A transgenic plant is obtained by heterologous transformation into arabidopsis thaliana through an agrobacterium tumefaciens-mediated method. Compared with wild arabidopsis thaliana, the weight, the length, the width and the surface area of the transgenic plant seeds are obviously improved. In addition, the cotyledon area of a transgenic plant is obviously higher than that of a wild type. Therefore, the MYB transcription factor MdMYB41L provides a new thought in the aspects of improving the plant seed size and molecular breeding.

The invention discloses a method for realizing efficient in-vitro regeneration of tea tree cotyledons through a somatic embryo indirect generation way, and belongs to the technical field of plant tissue culture. The tea tree somatic embryo indirect generation system is successfully constructed by screening culture conditions, a culture medium formula and other methods, and a foundation is laid for construction of a tea tree genetic transformation system and industrialized seedling production; according to the method, immature cotyledons of tea trees are used as explants, the cotyledons are induced to generate calluses, then the calluses are proliferated and differentiated to generate somatic embryos, and the somatic embryos are further differentiated to form complete plants, so that efficient and synchronous propagation of tea tree seedlings is realized, and technical support is provided for high-quality variety propagation and new variety breeding of tea trees; the problems of low propagation coefficient, long growth cycle, serious descendant degeneration and the like in traditional seed propagation of tea trees are solved, and the technical bottlenecks of serious explant browning, high bacterial contamination rate, poor plant growth synchronism and the like in other tissue culture technologies are solved.

The application discloses a method for high-frequency regeneration of Rhododendron chrysanthum based on cotyledon node indirect organogenesis, and belongs to the field of plant biotechnology. The method takes the cotyledon node of Rhododendron chrysanthum sterile seedling as an explant, and obtains a complete regenerated plant through the induction of callus, differentiation of adventitious buds, elongation of adventitious buds, strong seedling and rooting culture in sequence. The application realizes the super-high-frequency differentiation of adventitious buds by combining the cotyledon node explant with a specific hormone combination for the first time, the differentiation coefficient is as high as 96, and the rooting rate is 92.7%, thereby effectively solving the technical bottleneck of low propagation efficiency of Rhododendron chrysanthum, and providing reliable technical support for the conservation and industrialization development of Rhododendron chrysanthum germplasm resources.

The invention belongs to the field of gene engineering, and discloses a method for rapidly evaluating the plant growth capacity of transgenic herbicide-resistant cotton in the seedling stage, and the method comprises the following steps: S1, sowing transgenic cotton seeds to be detected; s2, when cotton cotyledons are completely flattened, glyphosate with the working concentration of 0 time or 2 times or 4 times is sprayed; s3, 7 days after spraying, respectively taking the overground part and rhizosphere soil of the cotton, and detecting the dry weight of the overground part and the water content and microbiome of the rhizosphere soil; and S4, judging the plant growth capability of the transgenic herbicide-resistant cotton in the seedling stage. The method provided by the invention is a rapid, standardized and repeatable seedling stage plant growth ability evaluation method, is suitable for multi-material and large-scale determination, is easy to popularize and apply in the agricultural field, and has urgent and important practical significance for accelerating glyphosate-resistant cotton variety breeding and industrial application.

The application belongs to the field of in vitro rapid propagation of Chinese medicine seedling, and particularly relates to a high-efficiency liquid suspension culture method of pinellia ternate, which comprises obtaining of pinellia ternate explants under sterile conditions, induction of embryogenic callus, liquid suspension culture, somatic embryo development and maturation, somatic embryo germination and transplanting of regenerated seedlings. By using the method, the induction rate of pinellia ternate embryogenic callus can reach 81.6%, stable mature cotyledon embryos can be obtained, and the embryos can be regenerated into complete plants, a high-efficiency and stable regeneration system is successfully established, favorable conditions are created for industrial production of pinellia ternate fine seedlings, and a good foundation is laid for genetic transformation of pinellia ternate.

This invention discloses a method, system, and device for branching the gene sequence of influenza A virus, relating to the field of bioinformatics. The method includes the following steps: reading a user-input phylogenetic tree structure file and a Fasta file; dividing the phylogenetic tree into multiple branches based on a set first custom parameter and the gene sequence data of each cotyledon node; verifying the relationship between branches based on a set second custom parameter, and merging branches whose genetic distance is within a set threshold; verifying the merged branches; and if there are unassigned cotyledon nodes, assigning them to the branch with the closest genetic distance that satisfies the custom parameter, thereby obtaining the final branching result of the influenza A virus gene sequence. The method employed in this invention enables targeted phylogenetic branching based on the characteristics of influenza A virus, improving the accuracy of the branching results.

The application belongs to the field of plant biotechnology, and particularly relates to a cucumber CsODO1 gene and application thereof, and is a MYB transcription factor capable of being combined with a CsCSE1 promoter, which is screened through a yeast single hybridization. It is proved through EMSA and tobacco transient expression analysis that the CsODO1 gene directly and specifically combines with a "CAACCA" sequence in the CsCSE1 promoter, and inhibits the expression of the CsCSE1 gene. The CsODO1 gene can be induced to express by a powdery mildew fungus, and is differentially expressed in the cucumber infected by the powdery mildew fungus. It is found through a cucumber cotyledon transient transformation system mediated by an agrobacterium that the CsODO1 gene negatively regulates the biosynthesis of lignin and the resistance of the cucumber to the powdery mildew fungus. The application provides a new reference gene resource for the cultivation of a cucumber disease-resistant variety, and provides a new thought for the research on the molecular mechanism of the plant immunity mediated by the lignin pathway.

The invention relates to a construction method of an efficient regeneration system of idesia polycarpa, which comprises the following steps: soaking idesia polycarpa seeds in 3% gibberellic acid diluted by 1000 times for 3 days, sterilizing, inoculating to a germination culture medium of 1 / 2MS, 1.0 mg / L 6-benzylamino adenine (6-BA) and 0.5 mg / L 3-indolebutyric acid (IBA), and culturing for 25 days to obtain seedlings; a hypocotyledonary axis at the position 2 cm below the cotyledon of the seedling is obliquely cut and inoculated to a culture medium containing MS, 1.0 mg / L of thidiazuron (TDZ) and 0.5 mg / L of IBA, and calluses are induced after 30 days; inoculating the calluses to a culture medium containing MS, 2.0 mg / L TDZ and 0.05 mg / L IBA, and differentiating regenerated buds after 30 days; after seedling strengthening and rooting culture, the seedlings are transplanted to a sterilization matrix with the ratio of nutrient soil to vermiculite to perlite being 4: 1: 1. The differentiation rate of regenerated seedlings reaches 64.82% and is remarkably higher than that in the prior art, the seed germination rate and the transplanting survival rate are high, and an idesia polycarpa regeneration system can be efficiently constructed.

The invention relates to the technical field of plant breeding, and discloses a culture medium and method for promoting masson pine somatic embryo maturation, masson pine embryogenic callus is inoculated into a somatic embryo maturation culture medium containing betaine, polyvinylpyrrolidone or dimethyl thiourea for somatic embryo induction culture, and the masson pine somatic embryo can be obviously promoted to mature in advance. Test results show that morphological observation is carried out on the embryogenic callus at different somatic embryo induction time points, when the embryogenic callus is cultured for 60 days, most cotyledon embryos are mature and a large number of mature embryos appear, the number of embryos is counted, the number of mature somatic embryos is 250-3500 / g embryogenic callus, and the number of total embryos is 3333-9000 / g embryogenic callus; the method can efficiently promote maturing of the masson pine somatic embryo, the somatic embryo induction efficiency is high, and the method has good practicability.

The invention discloses a method for establishing a gleditsia sinensis tissue culture regeneration system, and belongs to the technical field of plant tissue culture. Comprising the following steps: (1) inoculating explants from wild gleditsia sinensis seeds into an adventitious bud induction culture medium, and performing induction culture to generate adventitious buds; and (2) cutting off the adventitious bud from the base, inserting the cut end into a root induction culture medium, and inducing rooting. According to the method, hypocotyls and cotyledons of gleditsia sinensis aseptic seedlings are used as explants, and the influence of different plant growth regulator combinations, browning inhibitors and the like on the regeneration efficiency is discussed. According to the method, an efficient gleditsia sinensis regeneration system is successfully established, and a theoretical basis and a technical support are provided for high-quality seedling breeding and genetic engineering genetic improvement.

The invention discloses a culture method for rapid rooting of gingko aseptic seedlings, and relates to the technical field of gingko tissue culture. The culture method for rapid rooting of the gingko aseptic seedlings comprises the following steps: inoculating the gingko aseptic seedlings into a first rooting culture medium for culture, and then transferring the gingko aseptic seedlings into a second rooting culture medium for culture, the second rooting culture medium is an MS culture medium containing 0.1 to 0.2 mg / L of 6-benayl aminopurine, 0.5 to 1.5 mg / L of indolebutyric acid, 15 to 25 mL / L of gingko sterile main root juice, 7 to 9 mL / L of gingko sterile cotyledon juice, 25 to 35 g / L of sucrose and 6 to 8 g / L of agar. The invention effectively solves the problems of lack of staged regulation and control, single culture medium component, poor functionality, unreasonable hormone proportion and the like in the prior art.

The application discloses a kind of similar bundle-like arabino-galactoside protein coding gene GhFLA2 In the application of regulating cotton seed vigor, the application obtains several overexpression materials, RNA interference materials and gene knockout materials by transgenic technology GhFLA2 It is found that overexpression GhFLA2 Can promote radicle elongation during seed germination and cotyledon unfolding after germination, GhFLA2 After the expression amount of the application is reduced or knocked out, radicle elongation and cotyledon development are inhibited.The application provides a new gene target for cotton seed vigor improvement.

This invention relates to polynucleotides comprising a LEAFY COTYLEDON 1 (LEC1A) open reading frame in antisense orientation and expression cassettes, vectors, and compositions comprising the same for the purpose of enabling rapid somatic regeneration in a nonembryonic cell, as well as methods of making and methods of using the same.

The application provides a method for inducing transgenic hairy root formation of Lagenaria vulgaris by non-tissue culture, comprising the following steps: (1) Lagenaria vulgaris culture: sowing Lagenaria vulgaris seeds in a seedling substrate, and culturing in a light incubator until the Lagenaria vulgaris plant grows to a stage between one heart and one leaf and one heart; (2) infection by a hole-punching and smearing method: taking the cotyledon node to 1 cm below the cotyledon node of the Lagenaria vulgaris plant as an infection site, punching a hole around the infection site, and smearing the hole with Agrobacterium rhizogenes; (3) nodule induction: continuing to culture the Lagenaria vulgaris plant in the light incubator, and observing that the infection site is swollen; and (4) inducing hairy root formation by cutting and culturing: obliquely cutting the swollen position of the infection site of the Lagenaria vulgaris plant, and inserting the lower end of the obtained upper plant, i.e. the obliquely cut end, into a growth carrier for culture to induce hairy root formation. The application can induce transgenic hairy root formation of Lagenaria vulgaris, has a high induction rate, and can be applied to promoter function research, functional gene analysis, secondary metabolite research and the like.

The application discloses a tissue culture method for inducing adventitious bud regeneration by taking peach leaf as an explant. The application solves the problem of difficult peach adventitious bud regeneration, and the adventitious bud induction medium of the application successfully induces adventitious buds from the cotyledon of mature peach seeds. After bud elongation culture and adventitious root induction, the complete regenerated plant tissue culture seedlings are formed, and further through seedling hardening, normal growth seedlings are obtained. The application takes the cotyledon of mature peach seeds as the explant, has the advantages of simple disinfection and sterilization method, low cost, easy explant sampling, no season limitation, annual repetition, high regeneration efficiency, and provides technical support for the construction of a stable genetic transformation system of peach, is the basis of peach molecular breeding, and has wide application prospect in variety improvement.

The invention discloses application of a soybean histone variant to improvement of genetic transformation efficiency of soybeans and other plants. By applying the soybean histone variant, the infection efficiency of agrobacterium to soybean cotyledonary node explants can be remarkably improved, so that efficient introduction of exogenous genes is promoted, the transformation efficiency is remarkably improved, finally, the higher stable transformation rate is obtained, and the application can be expanded to recalcitrant plant species with genetic transformation difficulty such as tea. In addition, the subsequent genetic transformation efficiency can also be remarkably improved by using the genetic transformation material for stable overexpression of the soybean histone variant as a receptor.

The application belongs to the field of genetic engineering, and provides a Ghicr24CRU_D08 promoter and application thereof, wherein the nucleotide sequence of the promoter is shown as SEQ ID No. 1. The promoter provided in the application can drive specific expression of a target gene in cotyledon and root of a plant in the late stage of seed development (10-15DPA), the germination stage (imbibition 0-24h) and seedling (0-14d).

This invention belongs to the field of mung bean sprout growth analysis technology, specifically relating to a method and system for image recognition of mung bean sprout growth status. This invention divides the mung bean sprout into stem axis, cotyledon, and root tip regions, allowing for independent modeling of the growth characteristics of different functional regions. This improves the ability to analyze complex growth morphologies. By extracting multi-dimensional dynamic parameters such as length, diameter, angle, color, position, and density, a comprehensive quantitative description of the mung bean sprout growth process is achieved. This overcomes the limitations of traditional methods that rely solely on static features. By performing spatiotemporal unified processing on multi-time-series image data, and combining growth trajectory construction, structural feature analysis, and dynamic parameter modeling, standard growth reference information is introduced for comparative analysis. This enables continuous identification and anomaly judgment of mung bean sprout growth status, effectively improving the accuracy, stability, and interpretability of the identification.

The application discloses application of a soybean ARGONAUTE family gene GmAGO5. A soybean GmAGO5 protein coding gene GmAGO5 has a nucleotide sequence as shown in SEQ ID NO. 1. A plant gene editing knock-out vector pSCM-GmAGO5 is constructed and transformed into a receptor material Tianlong No. 1 by using a cotyledon node transformation method. By knocking out the GmAGO5 gene, the gene edited soybean plant height is reduced, the branch angle is reduced, and the soybean seed protein content is significantly improved. It can be seen that the soybean GmAGO5 protein coding gene GmAGO5 can be applied to reducing the soybean plant height, reducing the branch angle and improving the soybean seed protein content by means of genetic engineering.

The present application relates to the field of plant growth regulator technology, and in particular, to a phenylurea compound, a method for preparing same, and use thereof, and a composition for promoting crop cell division and a method for using same. The phenylurea compound provided herein has cytokinin-like biological activity and can effectively promote the germination of crop seeds and the expansion of cotyledons, increase the chlorophyll content, and retain and enlarge the fruits, featuring a stable action effect, safety to crops, and the like. Experimental results show that the phenylurea compound of the present application can effectively promote the germination of rice and peanut seeds, expansion of radish and cucumber cotyledons, leaf pigment retention and yield increase of peanuts, fruit set and retention of zucchini, and enlargement and sweetness enhancement of grape fruits, etc., exhibiting cytokinin-like effects.

The invention discloses a whole-tray type large seed vertical directional seeding method and device for factory plug seedling, machine vision and a mechanical device are combined, accurate directional seeding operation of seeds is realized through direction-adjusting migration after whole-tray type pre-seeding, and the whole set of device comprises a conventional matrix plug conveying belt and a direction-adjusting seeding device, the device is composed of a parallelogram connecting rod mechanism, four array type seed storage containers with different stations, three whole-disc type negative pressure seed suction devices responsible for seed movement between the different stations, a camera and a control system. After the seeds are taken from the seed box by negative pressure, four actions of pre-sowing, horizontal direction adjusting pre-sowing, vertical seed falling direction adjusting and seed pressing are performed in sequence, so that precise directional sowing of 45-degree circumferential positioning relative to the center of each hole and vertical insertion into matrix soil is realized, the orientation consistency of cotyledons in the seedling stage is ensured, and the optimal growth space and illumination are obtained. The seeding efficiency and quality are high, and convenient conditions are provided for subsequent accurate grafting.

The application relates to the tobacco seedling breeding technical field, and particularly discloses a leaf cutting device for tobacco seedling raising, which comprises a lifting flat plate arranged on a floating seedling raising tray; a plurality of insertion holes are linearly arranged along the length direction and the width direction of the lifting flat plate; a leaf cutting cover is inserted into the insertion holes and is provided with a seedling emergence hole along the axial direction of the leaf cutting cover; wherein, tobacco seedlings pass through the insertion holes and the leaf cutting cover and extend out of the seedling emergence hole, and the leaf cutting cover is in the shape of a circular truncated cone with a large bottom diameter and a small top diameter. In the application, the circular truncated cone-shaped leaf cutting cover corresponds to the seedling raising hole one by one, the stems of the tobacco seedlings can pass through the leaf cutting cover and extend out of the seedling emergence hole during growth, and the normal growth of the tobacco seedlings is ensured. When the tobacco seedlings grow to a proper size, the top of the leaf cutting cover is driven by the lifting flat plate to generate a shearing force on the lower leaves and the cotyledon of the tobacco seedlings, so that the leaves are forcibly cut off, thereby solving the problem that the lower leaves and the cotyledon of the tobacco seedlings cannot be cut off by traditional mechanical leaf cutting, and the problem of increased redundant nutrient consumption is solved.

The invention discloses a grafted rootstock seedling treatment device which comprises a melon seedling upper cotyledon beveling module, a melon seedling growing point drilling module, a cleft grafting rootstock cutting module, a lower treatment left side module and a rootstock treatment upper side module frame body. The melon seedling upper cotyledon beveling module and the melon seedling growing point drilling module are mounted on the inner side of the stock treatment upper module frame body; the cleft grafting rootstock cutting module and the lower processing left side module are correspondingly arranged in position and height, the cleft grafting rootstock cutting module is installed below the melon seedling upper cotyledon beveling module, and the lower processing left side module is installed below the melon seedling growing point drilling module; the rootstock processing upper module frame body is connected with the rack body. According to the device, stock seedlings can be cut or drilled through different mechanisms or modules in different treatment modes according to grafting requirements, and the diversified requirements of stock treatment are better met.

The invention discloses an application of an RsERF109a gene in regulation and control of yellowing and aging of radish sprouting vegetable leaves, and is characterized in that a nucleotide sequence of the RsERF109a gene is shown as SEQ ID NO.1, an amino acid sequence of protein encoded by the RsERF109a gene is shown as SEQ ID NO.2, aging of the radish sprouting vegetable leaves is delayed by inhibiting expression of the RsERF109a gene, and the application in plant breeding comprises construction of overexpressed RsERF109a radish sprouting vegetable. The invention also provides an application of the RsERF109a gene in preparation of a chlorophyll degradation related gene expression promoter. The RsERF109a gene has the advantages that the key function of the radish AP2 / ERF family transcription factor RsERF109a in regulating and controlling postharvest yellowing and senescence of the radish sprouting vegetable is identified and verified for the first time, postharvest yellowing of cotyledons of the radish sprouting vegetable can be remarkably accelerated by overexpressing the RsERF109a gene in the radish sprouting vegetable, and the postharvest fruit and vegetable quality can be accurately regulated and controlled through a molecular means.

The invention discloses an IBN transcription factor and application of a coding gene thereof in soybean branching promotion and yield increase, and belongs to the field of molecular biology and gene engineering. The IBN gene is obtained through cloning, an agrobacterium tumefaciens-mediated soybean cotyledonary node transformation method is utilized to obtain an IBN-overexpressed transgenic line, and research on functions of the IBN-overexpressed transgenic line shows that the branch number and the yield of a single plant of the IBN-overexpressed transgenic line are remarkably increased compared with those of a contrast. The discovery reveals that the IBN gene participates in a molecular mechanism for regulating and controlling soybean plant type establishment, and important gene resources and theoretical basis are provided for directionally improving soybean yield traits through molecular breeding, so that the IBN gene has a wide industrial application prospect, and good economic benefits can be generated.