The invention relates to a hydrosoluble reduced enzyme Q10 composition and a preparation method thereof, and provides a hydrosoluble reduced enzyme Q10 composition, which comprises raw materials and following mass components thereof: a reduced enzyme Q105-15, a hydrosoluble carrier 10-120, and an emulsifier 0.5-40; wherein the hydrosoluble carrier is one or more of maltose, CMC, gum rubber, soluble starch, lactose, fructose, sorbierite, mannitol, acacia and carrageenan; and the emulsifier is nonionic surfactant. The invention further provides the preparation method of the hydrosoluble reduced enzyme Q10 composition. In the method, the indissoluble reduced enzyme Q10 is mixed with the hydrosoluble carrier and the emulsifier to prepare the hydrosoluble reduced enzyme Q10 composition, advantages of an inclusion technology and a solid dispersion technology are synthesized, defects in the technologies are solved, a problem of slightly solubility of the reduced enzyme Q10 is solved, a series of tests shows that the reduced enzyme Q10 has water-solubility, high solubility, high dissolution rate, high stability and high active substance content.