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The present application relates to the technical field of collagen, in particular to a kind of active collagen lyophilized preparation and its preparation method and application.The preparation method includes the following steps: S1, the aqueous solution of freeze-drying protective agent is prepared;S2, the initial emulsion of active collagen is diluted with physiological saline, to obtain the first solution, then mixed with the aqueous solution obtained in step S1, to obtain the second solution;S3, the second solution obtained in step S2 is added to the aqueous solution of step S1, and the third solution is prepared after mixing and emulsifying, to obtain mixed emulsion;S4, the mixed emulsion in step S3 is filled and freeze-dried, wherein the freeze-drying protective agent includes glycine, and the concentration of active collagen in the third solution is 5-15 mg / mL.The lyophilized preparation prepared by the preparation method has excellent reconstitution effect, and is convenient for transportation and storage.