4results about How to "Good resolubility" patented technology

The application discloses a vaccine heat-resistant protective agent, a preparation method and application thereof, and belongs to the technical field of vaccines. The vaccine heat-resistant protective agent comprises the following raw materials: polycarboxybetaine, taurine, a sugar substance, polysarcosine, erythritol, gelatin enzymatic hydrolysate, a metal ion chelating agent and a buffer. The application also provides a preparation method. Compared with the prior art, the synergistic effect of polycarboxybetaine, taurine, a sugar substance, polysarcosine, gelatin enzymatic hydrolysate, erythritol and EDTA-Na2 can significantly improve the freeze-dried cake forming and reconstitution performance, shorten the reconstitution time, reduce the turbidity and insoluble particles. After 2-8 DEG C, 25 DEG C and 37 DEG C storage and multiple freeze-thaw cycle investigations, the antigen content residual rate and glass transition temperature are obviously superior to those of a traditional sucrose and mannitol system, and the inactivation risk caused by cold chain fluctuation can be effectively buffered.

The present application relates to the technical field of collagen, in particular to a kind of active collagen lyophilized preparation and its preparation method and application.The preparation method includes the following steps: S1, the aqueous solution of freeze-drying protective agent is prepared;S2, the initial emulsion of active collagen is diluted with physiological saline, to obtain the first solution, then mixed with the aqueous solution obtained in step S1, to obtain the second solution;S3, the second solution obtained in step S2 is added to the aqueous solution of step S1, and the third solution is prepared after mixing and emulsifying, to obtain mixed emulsion;S4, the mixed emulsion in step S3 is filled and freeze-dried, wherein the freeze-drying protective agent includes glycine, and the concentration of active collagen in the third solution is 5-15 mg / mL.The lyophilized preparation prepared by the preparation method has excellent reconstitution effect, and is convenient for transportation and storage.

The invention provides a creatine kinase isoenzyme freeze-dried product as well as a preparation method and application thereof. The creatine kinase isoenzyme freeze-dried product is prepared from the following raw materials: a creatine kinase isoenzyme antigen, polygonatum polysaccharide and a chelating agent; the chelating agent comprises any one of citric acid, iminodiacetic acid and 1-hydroxyethylidene-1, 1-diphosphonic acid. The components such as the polygonatum polysaccharide and the chelating agent are adopted, so that double protection of oxidation resistance and dimer stability is realized, and the creatine kinase isoenzyme is good in activity after freeze-drying, stable in long-term storage and good in detection accuracy and repeatability.