114 results about "Biotechnology research" patented technology

The invention discloses a manufacturing technique for preparing frozen-out gene engineering bacteria competent cells. The invention also relates to a prescription of protecting agent, which is wide in application by taking as the host bacteria for transferring outer DNA in researching, developing, producing and checking of biotechnology. The protecting agent is one of the most fundamental matching consumption preparations. The invention utilizes vacuum freeze drying technology to deal with the poor gene engineering bacteria competent cells, and can save the cells steadily at a wide temperature range of 20 DEG C below zero to 4 DEG C for long time while keeping high transformation efficiency. The invention enables the cells to store and long-distance transport conveniently. The invention relates to a manufacturing technique for preparing frozen-out competent cells, quality inspection regulation and the frozen-out protecting agent, comprising culture conditions of gene engineering bacteria, technological processes of freezing and drying and the composition and matching of the protecting agent. The protecting agent is formed by water and one or arbitrary combination of the following materials: gelatin, degreasing milk, dextran, trehalose, sucrose, sorbitol or mannitol.

The invention belongs to the technical field of biotechnology research and development, and particularly relates to a test tube cleaning, disinfecting and drying device for biotechnology research anddevelopment. Aiming at the problems that test tubes for biotechnology research and development are mostly manually cleaned, the cleaning efficiency is low, meanwhile, disinfecting and drying are needed after cleaning, and consequently the biotechnology research and development cost is increased, the test tube cleaning, disinfecting and drying device is characterized in that the test tube cleaning,disinfecting and drying device comprises a recovery box; the top of the recovery box is connected with a cleaning box through bolts, and a box cover is hinged to one side of the top of the cleaning box; and vertically-formed mounting grooves are formed in the two ends of the inner walls of the two sides of the cleaning box correspondingly, and mounting blocks are arranged in the four mounting grooves correspondingly. Cleaning, disinfecting and drying of the test tubes are achieved through the same device, thus the recovery cost of the test tubes for biotechnology research and development canbe saved, meanwhile, a supporting mesh plate can ascend through a second linear electric pushing rod, a cleaning pipe can be lifted outside the cleaning box, taking and placing of the test tubes are facilitated, and great convenience is brought to biotechnology research and development personnel for recovering the test tubes.

The invention discloses a RNA has-miR-29c related to Hepatitis B Virus (HBV) and liver cancer and its uses in inhibiting HBV infection and preventing liver cancer from generation. The invention is directed to the field of biology technology research. Based on qRT-PCR technology, it is found that compared to a hepatocyte lineage HepG2, the has-miR-29c has a down-regulation of expression in a hepatoma cell line. A bioinformatics method and double fluorescence detection are used to determine that the target gene of has-miR-29c is Tnfaip3. The has-miR-29c expression carrier is externally transfected. It is observed that miR-29c can inhibit the growth of tumor cells for a short period and has a certain inhibition effect to the duplication and expression of HBV DNA. The target gene Tnfaip3 has a down-regulation of expression both on mRNA and the level of protein. The research result shows miR-29c possibly participates in the generation and development process of liver cancer related to HBV through its target gene Tnfaip3. Therefore, miR-29c can be used as a regulation and control molecule for inhibiting HBV infection and preventing liver cancer from generation, thereby providing a theoretical and experimental basis for clinic prevention and treatment.

The invention discloses a callus culture method by taking Rhizoma Paridis roots and stems as explants. The method provided by the invention takes specially pre-treated Rhizoma Paridis roots and stems as the explants; and the method finally realizes an effective induction and a rapid propagation on the Rhizoma Paridis plant calluses by inoculating the explants into a callus induction culture medium and a propagation culture medium to be cultured after disinfecting the explants. The method provided by the invention better solves the bottleneck problem of the traditional Rhizoma Paridis plant onthe aspect of tissue culture and lays a substantial theoretical foundation on carrying out all biotechnology researches on the Rhizoma Paridis plant in a deep-going way in future.

The invention belongs to the field of biotechnology research, and particularly relates to an elastin-like polypeptide (ELP) and heat stress protein 90alpha (Hsp90alpha) fusion protein, and a preparation method and application thereof in infectious bursal disease virus (IBDV) concentration and purification. The ELP and Hsp90alpha fusion protein is composed of an IBDV binding segment M167a of ELP and Hsp90alpha. The preparation method comprises the following steps: construction of fusion expression vector, expression and purification of fusion protein, determination of IBDV binding segment, and concentration and elution of IBDV. Compared with other processes, the fusion protein for concentrating and purifying the IBDV is simple, and has the advantages of high speed and high economy. The prepared IBDV can be used for experimental research and vaccine preparation.

The invention discloses a creation method of switchgrass mutants. The creation method comprises the following steps: after a lots of switchgrass multiple shoots are cultured in a tissue culture manner, stripping out individual multiple shoots, cutting off the upper sheaths after acclimatization under natural conditions, performing soaking treatment on the parts 1.5-2cm above the left base parts by use of a solution of ethylmethane sulfonate (EMS) for 3 hours, and after the completion of the soaking treatment, transplanting the multiple shoots of which the sheaths are cut off into a culture medium for culture, and after complete rooting, transplanting to a greenhouse or land, and screening out the mutants according to the phenotypes of the switchgrass. The method is capable of realizing large-scale processing of the mutational original acceptor material and thus increasing the basic number of the variants, and meanwhile, a near-isogenic line material only different from the original acceptor material in target traits can be obtained; and in addition, the mutagenesis process is simple and feasible, the processing cost is relatively low, and the breeding period can be effectively shortened; in short, the method is suitable for creating new materials for the modern biotechnology research of the switchgrass.

The invention belongs to the field of medical biotechnology research, and discloses a circulating nerve cell detection kit containing a fluorescently-labeled neuron specific antibody, a fluorescently-labeled white blood cell classic marker antibody and a cell nucleus fluorescent dye on the first hand. Besides, the invention discloses a density gradient centrifugation kit for detecting circulatingnerve cells and a detection method of the kit. Furthermore, the invention discloses an immunomagnetic bead kit for detecting circulating nerve cells and a detection method of the immunomagnetic bead kit. Compared with the conventional detection means, the technical scheme disclosed by the invention has the technical effects of high specificity, simplicity in detection and sampling, lower detectioncost and price and capability of realizing real-time monitoring of a blood sample.

The invention belongs to the technical field of culture devices, and in particular relates to a constant temperature cell culture device for biotechnology research and development. The constant temperature cell culture device aims at solving the problem that the existing culture device is simple in structure, single in function and poor in culture effect. The culture device comprises a base. The bottom outer wall of the base is provided with a groove, and sliding rails are fixed on the inner walls on two sides of the groove through screws. The inner walls of the sliding rails are in sliding connection with sliding blocks, a horizontally arranged mounting plate is welded on the outer walls of the opposite sides of the two sliding blocks, universal wheels are fixed at four corners of the bottom outer wall of the mounting plate through screws, and a sterilization box is fixed on the top outer wall of the base through screws. The culture device achieves good culture effect and high intelligence degree, significantly improves the culture efficiency of cells, ensures the culture quality of the cells, greatly reduces the culture time, significantly prolongs the service life and ensures culture of the cells under aseptic condition.

The invention discloses a method for analyzing a regulation effect of deer skin protein peptide on macrophage immune cells, and belongs to the technical field of biotechnology research, development and analysis. By compared with proliferation activities of samples with different concentrations, a result shows that the proliferation index of RAW264.7 cells is increased along with the increase of adeer skin protein peptide concentration, and the concentration is 200[mu]g/mL and is closest to an LPS positive control group; in order to verify the influence of deer skin peptide on immunoregulation, the influence of deer skin protein peptide with different mass concentrations on NO secretion amount, LZM activity and release of cytokines such as IL-6 and TNF-[alpha] is researched, and the resultshows that the NO secretion amount is increased along with the increase of the sample concentration; after the deer skin protein peptide acts for 24 hours, the secretion function of RAW264.7 cytokines is obviously increased, and when the concentration is 50 [mu]g/mL, the levels of all the cytokines are close to those of an LPS group, which indicates that the deer skin protein peptide has the effect of enhancing cytokine secretion; in conclusion, the deer skin protein peptide has a potential effect of enhancing the immunocompetence, and can provide reference for subsequent research.

In the present invention, a biological reagent is fixed on the first supporting body and the biological reagent fixed on the first supporting body is contacted with biological molecule fixed on the second supporting body when the dot array is used to detect the biological molecule fixed on the second supporting body. At least one biological reagent is combined with biological molecule on the second supporting body, and then the first supporting body is separated from the second one as at least one biological reagent is separated from the first supporting body to combine on the second supporting body.

The invention belongs to the field of biotechnology research, and particularly relates to a TEVP (Tobacco Etch Virus Protease) active inclusion body as well as a preparation method thereof and application thereof in the preparation of a recombinant protein. The TEVP active inclusion body consists of an ELK16 self-polymeric peptide and TEVP that are separated by two PT connectors. The preparation method of the TEVP active inclusion body comprises the steps of an expression vector construction, an active inclusion body expression and purification, recombinant protein cleavage and target protein recovery. Compared with existing TEVP, the TEVP active inclusion body proviced by the invention has the advantages of simple preparation, easiness for being removed from an end product, low cost and the like, and can be applied to prepare various recombinant proteins.

The invention belongs to the field of biotechnological research, and particularly relates to long-acting interferon and a preparation method thereof. The long-acting interferon is composed of interferon and streptococcal G protein C2 structural domain, and the preparation method includes the steps of fusion expression vector, fusion interferon expression and purification, purification tag removal and fusion interferon recovery. Compared with the existing recombinant interferon, the long-acting interferon has the advantages of being simple in preparation process, low in cost, long in half-life period and the like, and can be used for the preparation of long-acting interferon for human and animals.

The invention belongs to the bio-technical field, and particularly relates to an air purifying device with a humidifying function based on the biological technology. In order to solve the problems that the air purifying device is complex in structure, is poor in air purifying effect, is single in function, and cannot meet requirements very well, the invention discloses the scheme that the air purifying device comprises a bottom box, wherein the four corners of the outer wall of the bottom of the bottom box are connected with universal wheels through bolts; and the outer wall of one side of thebottom box is connected with two connecting frames which are symmetrically arranged through bolts. The air purifying device realizes a humidifying function, effectively improves the quality of air inside a biotechnical research room, effectively improves working efficiency of a biotechnical worker, conveniently regulates the direction of a gas inlet of a gas inlet tube according to a practical condition in a laboratory by a user, effectively improves use convenience of the device, conveniently replaces consumables such as a purifying agent, a toner cartridge and a drying agent inside the boxbody frequently by the user, and ensures air purifying efficiency of the device.

The invention discloses a multifunctional workbench for biotechnology research and development, and relates to the technical field of biotechnology research and development equipment. The device comprises a research and development workbench, a driving cavity is formed in the upper end in the research and development workbench, a storage driving motor is fixed in the driving cavity, a first bevel gear is fixed to the output end of the storage driving motor, a second bevel gear is assembled to the lower end of the first bevel gear, and a threaded rod is welded to the interior of the second bevel gear. Through mutual cooperation of a storage driving motor, a threaded rod and a classified storage mechanism, tools and reagents for research and development can be stored in a classified mode through the device, the workbench is clean and tidy, research and development personnel can conveniently take the tools and reagents needing to be used, the device is convenient to use, and the working efficiency is improved. And through mutual cooperation of the research and development workbench, hollow supporting legs, an H-shaped fixing plate and a lifting mechanism, the height of the device can be adjusted according to the height of research and development personnel, and the device is convenient to use.

The invention discloses a cell culture device for biotechnology research, and the cell culture device comprises a processing tank body; a plurality of supporting bottom feet are fixedly mounted at the bottom end of the processing tank body, the bottom ends of the supporting bottom feet are supported on the ground, and a processing cavity is arranged in the processing tank body; a tank body cover plate is fixedly connected into an opening in the top end of the machining cavity through a first thread locking structure, and a driving motor is fixedly connected to the outer wall of the top end of the tank body cover plate. According to the invention, by utilizing the arranged processing cavity, especially a transmission rod and a stirring rod arranged in the processing cavity, after the whole stirring rod is driven to rotate through the operation of the driving motor, cell culture materials entering the processing cavity are fully crushed and then are guided into corresponding culture drawers in batches; then the materials are guided into the corresponding culture drawers in batches, different culture materials can be researched separately, and the whole structure is reasonable in design, easy to operate, high in mechanization degree and worthy of later popularization and application.