6 results about "Biotechnology research" patented technology

The invention discloses an oomycete effect protein prediction method based on a protein language model and deep learning, and relates to the field of bioinformatics and machine learning. The method comprises the following steps: 1, collecting oomycete effect protein data and non-effect protein data; 2, obtaining protein embedding through a protein language model; 3, performing oversampling processing on the data; 4, constructing a deep learning model; 5, model training; and 6, performing cross validation and independent test evaluation. According to the invention, a geometric oversampling technology (G-SMOTE) is adopted to carry out oversampling processing, so that the problem of sample imbalance is solved; a protein language model is adopted to obtain protein embedding characteristics to strengthen protein expression; and the prediction accuracy is improved by using a deep learning model architecture. The method can be applied to agricultural disease prevention and control, drug development and biotechnology research, important support is provided for identification and research of oomycete effect protein, prediction accuracy can be improved, dependence on expensive experiments can be reduced, and labor cost can be reduced.

The invention belongs to the field of biotechnology research, and describes a method for monitoring tomato fusarium wilt and early prevention and treatment of diseases based on minimally invasive tissue sampling. The method comprises the following steps: puncturing a tomato stem base through a sterile syringe needle to obtain trace vascular bundle tissues, and performing rapid nucleic acid extraction; then, whether the plant is infected or not is judged 5-7 days before typical symptoms appear through an isothermal amplification nucleic acid detection technology, the positive detection rate of a loop-mediated nucleic acid isothermal amplification (LAMP) detection method is 47.6%, and the positive detection rate of a recombinase-mediated nucleic acid isothermal amplification (RAA) detection method is 89.71%; finally, for the detected positive plants, in the window period when disease symptoms do not appear, trifluoropyridylamine and bacillus velezensis JSBV63 are adopted for root irrigation treatment in time, the progress of plant diseases can be controlled and blocked, and the control effect can reach 90% or above after two times of pesticide application. The combination of minimally invasive sampling, isothermal nucleic acid amplification detection and medicament treatment realizes a whole set of complete fusarium wilt prevention and treatment technical process from early diagnosis of pathogen infection to disease treatment.

The invention provides a cleaning device special for intelligent instruments for biotechnology research and development, and relates to the technical field of cleaning equipment.The cleaning device comprises a flushing water tank, and a purification water tank is installed on the rear side of the flushing water tank; a water flow guide plate is mounted at the rear end of the inner side of the flushing water tank; a gear is mounted on the outer side of the rotating pipe, the gear is meshed with a gear at the output end of a driving motor on the front side of the flushing water tank, the outer end of the rotating pipe is rotationally mounted in the fixed cover, and a flow guide ring pipe is mounted at the inner end of the rotating pipe; nozzles are arranged on the side face of the flow guide ring pipe, and the flow guide ring pipe is located on the front side of the interior of the flushing water tank. According to the high-pressure cleaning device, the high-pressure water flow is combined with the strong impact force of the high-pressure water flow to the impurities, pollutants attached to the instruments can be rapidly and thoroughly flushed away, the flushing time of the single instrument is remarkably shortened, the overall cleaning efficiency is improved, and the problem that part of cleaned impurities can be attached to the surfaces of the instruments again along with the water flow, and the instrument cleaning efficiency is affected is solved.

The invention relates to a Fibroblast Growth Factor 2 (FGF-2) polypeptides with improved stability while maintaining biological activity compared to the wild-type of FGF-2. The invention provides the process for the preparation, stabilisation and the use thereof in biotechnological research and industrial applications, the pharmaceutical industry, cosmetics, clean meat industry, generation of organoids, 3D cell culture models, in combination with physical modalities and other related applications.

The application discloses a method for cultivating sterile seedlings by using pepper dry fruits, relates to the technical field of plant tissue culture, and particularly relates to a method for cultivating pepper sterile seedlings. The cultivating method comprises the following steps: after sterilizing pepper seeds, the pepper seeds are inoculated into a culture medium for cultivation; the sterilization is performed by using 75% alcohol and then 10% H2O2 or 20% NaClO; and the culture medium is composed of water and agar. The method takes the stored pepper seeds as explants to cultivate the sterile seedlings, and the material is not limited by time, is economic and environment-friendly, and is of great significance to pepper elite breeding, biotechnology research and subsequent high-efficiency factory seedling cultivation. The method for cultivating the pepper sterile seedlings is simple, environment-friendly, low in cost and high in germination rate.