114 results about "Biotechnology research" patented technology

The invention discloses a manufacturing technique for preparing frozen-out gene engineering bacteria competent cells. The invention also relates to a prescription of protecting agent, which is wide in application by taking as the host bacteria for transferring outer DNA in researching, developing, producing and checking of biotechnology. The protecting agent is one of the most fundamental matching consumption preparations. The invention utilizes vacuum freeze drying technology to deal with the poor gene engineering bacteria competent cells, and can save the cells steadily at a wide temperature range of 20 DEG C below zero to 4 DEG C for long time while keeping high transformation efficiency. The invention enables the cells to store and long-distance transport conveniently. The invention relates to a manufacturing technique for preparing frozen-out competent cells, quality inspection regulation and the frozen-out protecting agent, comprising culture conditions of gene engineering bacteria, technological processes of freezing and drying and the composition and matching of the protecting agent. The protecting agent is formed by water and one or arbitrary combination of the following materials: gelatin, degreasing milk, dextran, trehalose, sucrose, sorbitol or mannitol.

The invention relates to an array-type high-throughput microbe separating culturing apparatus, and discloses a novel microbe separating culturing apparatus. The apparatus is capable of realizing high-throughput separation and culture of cultured and / or uncultured microbes in a laboratory, operation is simplified, and the apparatus has good compatibility with conventional laboratory equipment. The separating culturing apparatus is capable of simulating in-situ culturing of microbe by natural environment, and is capable of efficiently separating difficult-to cultured microbe ( or named uncultured microbe or unculturable microbe). The microbe separating culturing apparatus is applicable to separate and culture difficult-to cultured microbe from environment, also is applicable to research interacting relationship between microbe and microbe, between microbe and environment factors, and between microbe and nutrition factors, and also is applicable to fields such as microbe source investigation and research, environment and ecology relationship, natural medicine discovery, biological technology research and the like.

The invention belongs to the technical field of biotechnology research and development, and particularly relates to a test tube cleaning, disinfecting and drying device for biotechnology research anddevelopment. Aiming at the problems that test tubes for biotechnology research and development are mostly manually cleaned, the cleaning efficiency is low, meanwhile, disinfecting and drying are needed after cleaning, and consequently the biotechnology research and development cost is increased, the test tube cleaning, disinfecting and drying device is characterized in that the test tube cleaning,disinfecting and drying device comprises a recovery box; the top of the recovery box is connected with a cleaning box through bolts, and a box cover is hinged to one side of the top of the cleaning box; and vertically-formed mounting grooves are formed in the two ends of the inner walls of the two sides of the cleaning box correspondingly, and mounting blocks are arranged in the four mounting grooves correspondingly. Cleaning, disinfecting and drying of the test tubes are achieved through the same device, thus the recovery cost of the test tubes for biotechnology research and development canbe saved, meanwhile, a supporting mesh plate can ascend through a second linear electric pushing rod, a cleaning pipe can be lifted outside the cleaning box, taking and placing of the test tubes are facilitated, and great convenience is brought to biotechnology research and development personnel for recovering the test tubes.

The present invention relates to a coumarin compound that is used for analysis of enzymatic activity and enzyme inhibitor screening. The compound has a structure formula as shown in the right; wherein, R<1> is an H atom, ester prepared from hydrogen of OH that is substituted by aliphatic carboxyl or aromatic carboxylic acid, or ether that is prepared from hydrogen of OH that is substituted by carboxyl of sterol, monosaccharide and polysaccharide; R <2>, R<3> and R<4> can be H, alkyl containing 1 to 18 carbon atoms, perfluorinated alkyl containing 1 to 18 carbon atoms, -CN,-Ar, miscellaneous aryl, carbonyl or carboxyl amide. The coumarin compound adopts Pechmann reaction or Knovenagel reaction to introduce halogen atoms in the sixth and eighth positions of a benzene ring, to prepare the di-halogenated 7-hydroxycoumarin compound. The coumarin compound can be used as a fluorescent probe for marking analysis in the biotechnological research, and has the characteristics of simple operation, high stability, high sensitivity, high selectivity, and so on. And the coumarin compound is mainly used for the analysis of enzymatic activity, the enzyme inhibitor screening, and the detection of cell activity.

The invention discloses a method for separating and preparing agarose from agar by using a polyethylene glycol precipitation method. The method comprises the following steps of: 1, preparing agar solution with an appropriate concentration, and heating, stirring and dissolving the agar solution into semitransparent solution; 2, putting the agar solution into a water bath boiler to keep the temperature constant; 3, preparing PEG solution with a certain concentration, and heating the PEG solution in a water bath to ensure that the PEG solution is in a completely dissolved state and the temperature is kept constant; 4, quickly missing the agar solution with the PEG solution, and stirring the mixture continuously until a large amount of white floccule deposits appear; 5, taking the mixed solution out of the water bath boiler, and standing the mixed solution for cooling; 6, centrifuging a suspension to obtain a white deposit; 7, repeatedly washing the deposit by using a great deal of distilled water, and finally washing the deposit by using acetone; 8, drying the deposit to obtain a white agarose initial product; and 9, repeating the steps 1 to 8 to obtain refined agarose. In the method, the agarose is prepared by further purifying agar powder, so the gel strength of the agarose is improved, the electro-endosmosis of the agarose is reduced, and the method is very necessary for promoting the development of biotechnology researches.

The invention provides a building method of a transposable element carrier expressing pig telomerase reverse transcriptase, and application in building a pig immortalization cell line, and belongs to the field of biotechnology research. The building method includes the steps of cloning of a promoter of a pig protein translation elongation factor 1a and detection of transcriptional activity, building of an SB transposable element expression vector and exogenous gene integration, pig telomerase reverse transcriptase cDNA cloning, activity detection and building of the pig fibroblast immortalization cell line. Compared with other methods, the pig immortalization cell line built through the transposable element carrier comprises non-transformed normal cells, does not contain resistance genes, virogenes or oncogenes, and is suitable for cell physiological function researching, pig virus separation cultivation and vaccine production.

The invention discloses a RNA has-miR-29c related to Hepatitis B Virus (HBV) and liver cancer and its uses in inhibiting HBV infection and preventing liver cancer from generation. The invention is directed to the field of biology technology research. Based on qRT-PCR technology, it is found that compared to a hepatocyte lineage HepG2, the has-miR-29c has a down-regulation of expression in a hepatoma cell line. A bioinformatics method and double fluorescence detection are used to determine that the target gene of has-miR-29c is Tnfaip3. The has-miR-29c expression carrier is externally transfected. It is observed that miR-29c can inhibit the growth of tumor cells for a short period and has a certain inhibition effect to the duplication and expression of HBV DNA. The target gene Tnfaip3 has a down-regulation of expression both on mRNA and the level of protein. The research result shows miR-29c possibly participates in the generation and development process of liver cancer related to HBV through its target gene Tnfaip3. Therefore, miR-29c can be used as a regulation and control molecule for inhibiting HBV infection and preventing liver cancer from generation, thereby providing a theoretical and experimental basis for clinic prevention and treatment.

The invention discloses a callus culture method by taking Rhizoma Paridis roots and stems as explants. The method provided by the invention takes specially pre-treated Rhizoma Paridis roots and stems as the explants; and the method finally realizes an effective induction and a rapid propagation on the Rhizoma Paridis plant calluses by inoculating the explants into a callus induction culture medium and a propagation culture medium to be cultured after disinfecting the explants. The method provided by the invention better solves the bottleneck problem of the traditional Rhizoma Paridis plant onthe aspect of tissue culture and lays a substantial theoretical foundation on carrying out all biotechnology researches on the Rhizoma Paridis plant in a deep-going way in future.

Provided is a zero-emission zero-pollution circular agricultural production system. The system comprises a biotechnology research and development center, a biomaterial production center, an organic planting base, an ecological aquaculture base, an ecological livestock breeding farm, an organic fertilizer factory, a special feed factory and a deep processing and refrigeration center. According to the zero-emission zero-pollution circular agricultural production system, an organic planting technology, an ecological aquaculture technology, an ecological livestock breeding technology, a deep processing and refrigeration technology, an organic fertilizer preparation technology and a special feed production technology are combined, and the purposes of zero emission, zero pollution and circular agriculture development are achieved; meanwhile, products produced in the system are safe, free of toxicity, green, environmentally friendly and free of pesticide residue, drug residue and antibiotic residue, and the effects of increasing both production and income, improving product quality and taste and the like can be achieved in the planting process; in the breeding process, the survival rate and the yield can be guaranteed, and the high-quality effect is achieved.

The invention belongs to the field of medical biotechnology research, and discloses a hepatitis B virus genotyping detection method based on a CRISPR / Cas13a system. The hepatitis B virus genotyping detection method comprises the following steps of 1, designing a crRNA sequence, and carrying out in vitro transcription synthesis; 2, designing a universal recombinant polymerase amplification (RPA) primer of the HBV genome DNA by using an NCBI Primer Blast online tool; 3, synthesizing and purifying hepatitis B virus RNA; 4, using Cas13a enzyme and HBV crRNA capable of recognizing HBV B-type and C-type viruses at the same time for sensitivity testing of the detection method; and 5, carrying out enzyme digestion fluorescence test on the plasmid containing the HBV B-type or-C type genome DNA by using Cas13a enzyme and crRNA. Compared with the conventional detection means, the scheme disclosed by the hepatitis B virus genotyping detection method based on the CRISPR / Cas13a system has the characteristics of simplicity in operation, high sensitivity and low price, and meanwhile, an additional hairpin structure is contained in the specific recognition area, so that the high detection specificity is improved, and the HBV genotype can be distinguished within 1-2h.

The invention discloses a honeysuckle protoplast separation and culture method and a special culture medium, and belongs to the technical field of plant tissue and cell culture. The method comprises the following steps: (1) disinfection of explants; (2) pre-treatment of the explants: pre-treating leaves in a plasmolysis solution for 60 minutes; (3) protoplast separation: mixing the leaves with anenzymatic hydrolysate, standing and carrying out enzymolysis for 4 hours, and then carrying out low-speed oscillation enzymolysis for 4 hours; (4) purification of a protoplast: purifying the separatedprotoplast by adopting a precipitation method; and (5) protoplast culture: carrying out liquid shallow culture by adopting a protoplast culture medium. Honeysuckle test-tube plantlet leaves are usedas explants, the honeysuckle protoplast with high yield and high activity is obtained through separation and purification, a protoplast culture system is preliminarily established, and the method hasgreat significance in development of honeysuckle crossbreeding, gene engineering and other related biotechnology researches.

The invention discloses Saccharothrix yanglingensis Hhs.015. The bacterial strain has been preserved in a China General Microbiological Culture Collection Center in June, 2009 with a collection number of CGMCC NO.4.5627; and the bacterial strain also has been preserved in a Type Culture Collection Center of the Korea Institute of Bioscience and Biotechnology in October, 2009 with a collection number of Saccharothrix yanglingensis Hhs.015T / KCTC 19722T. The invention also discloses a separation and identification method for the bacterial strain, a preparation method for thallus and a preparation method for crude antibacterial active extracts, and also discloses application of the Hhs.015 bacterial strain and the thallus and antibacterial active substances thereof in preventing and controlling the tomato leaf mold. The Hhs.015 bacterial strain has the advantages of stable natural raw material source, simple screening method, convenient operation, stable product quality, no toxic or side effect, environmental protection, obvious effect of preventing and controlling the tomato leaf mold, and convenient popularization and application.

The invention belongs to the field of biotechnology research, and particularly relates to an elastin-like polypeptide (ELP) and heat stress protein 90alpha (Hsp90alpha) fusion protein, and a preparation method and application thereof in infectious bursal disease virus (IBDV) concentration and purification. The ELP and Hsp90alpha fusion protein is composed of an IBDV binding segment M167a of ELP and Hsp90alpha. The preparation method comprises the following steps: construction of fusion expression vector, expression and purification of fusion protein, determination of IBDV binding segment, and concentration and elution of IBDV. Compared with other processes, the fusion protein for concentrating and purifying the IBDV is simple, and has the advantages of high speed and high economy. The prepared IBDV can be used for experimental research and vaccine preparation.

The invention discloses a creation method of switchgrass mutants. The creation method comprises the following steps: after a lots of switchgrass multiple shoots are cultured in a tissue culture manner, stripping out individual multiple shoots, cutting off the upper sheaths after acclimatization under natural conditions, performing soaking treatment on the parts 1.5-2cm above the left base parts by use of a solution of ethylmethane sulfonate (EMS) for 3 hours, and after the completion of the soaking treatment, transplanting the multiple shoots of which the sheaths are cut off into a culture medium for culture, and after complete rooting, transplanting to a greenhouse or land, and screening out the mutants according to the phenotypes of the switchgrass. The method is capable of realizing large-scale processing of the mutational original acceptor material and thus increasing the basic number of the variants, and meanwhile, a near-isogenic line material only different from the original acceptor material in target traits can be obtained; and in addition, the mutagenesis process is simple and feasible, the processing cost is relatively low, and the breeding period can be effectively shortened; in short, the method is suitable for creating new materials for the modern biotechnology research of the switchgrass.

The invention belongs to the field of medical biotechnology research, and discloses a circulating nerve cell detection kit containing a fluorescently-labeled neuron specific antibody, a fluorescently-labeled white blood cell classic marker antibody and a cell nucleus fluorescent dye on the first hand. Besides, the invention discloses a density gradient centrifugation kit for detecting circulatingnerve cells and a detection method of the kit. Furthermore, the invention discloses an immunomagnetic bead kit for detecting circulating nerve cells and a detection method of the immunomagnetic bead kit. Compared with the conventional detection means, the technical scheme disclosed by the invention has the technical effects of high specificity, simplicity in detection and sampling, lower detectioncost and price and capability of realizing real-time monitoring of a blood sample.

The invention relates to shaking-up equipment, in particular to multi-solution simultaneous stirring and shaking-up equipment for biotechnology research and development. To overcome technical problems in the prior art, the multi-solution simultaneous stirring and shaking-up equipment for biotechnology research and development provided by the invention has a clamping function and relatively high stability. According to a technical scheme in the invention, the multi-solution simultaneous stirring and shaking-up equipment for biotechnology research and development comprises: a base; a supporting assembly, which is arranged at the top of the base; four stirring barrels, which are uniformly placed on the supporting assembly at intervals; a stirring assembly, which is arranged at the top of the supporting assembly; and four stirring rods, which are uniformly arranged on the stirring assembly at intervals. According to the invention, the stirring rods move downwards into the stirring barrels, and groove drums and the stirring rods are driven to rotate under the action of clamping rods, so the stirring rods uniformly stir a solution, the effect of automatically stirring the solution is achieved, and the working efficiency of people is effectively improved.

The invention discloses a preparation method of a programmed death receptor 1 (PD-1) antibody magnetic bead. The preparation method comprises the following steps: respectively coupling 14 anti-human PD-1 monoclonal antibodies with a carboxyl magnetic bead to prepare a PD-1 antibody magnetic bead, wherein the 14 anti-human PD-1 monoclonal antibodies are from the Canada YES company biotechnology research limited company, and the clone numbers of the 14 anti-human PD-1 monoclonal antibodies are respectively 7E5, 1H8, 1C4, 9A5, 9B4, 7C9, 3E5, 7G12, 9E11, 5B2, 5H7, 2H7, B1C4 and 2C4. The inventionalso discloses the application of the above PD-1 antibody magnetic bead in the field of CIK (cytokine induced killer), CART (Chimeric Antigen Receptor T-Cell Immunotherapy) and TIL (tumor infiltrationlymphocyte) immunotherapy for rejecting PD-1 positive cells. According to the preparation method disclosed by the invention, a coupling scheme of antibodies and magnetic beds is optimized, so that the coupling amount of the antibody on the surface of the magnetic bead can achieve 1ml of magnetic bead+1mg of antibody, and a fluorescence signal on the surface of the magnetic bead is enhanced afterthe antibody is coupled. The antibody magnetic bead prepared with the preparation method can be used for capturing PD-1 positive cells.

The invention belongs to the technical field of biotechnology research and development, and particularly relates to a cleaning, disinfecting and drying device for test tubes for biotechnology researchand development. According to the cleaning, disinfecting and drying device, the problem of cleaning the interiors of the test tubes can be solved. According to the following scheme, the cleaning, disinfecting and drying device comprises a bottom plate, an ozone generator, a mixing tank and a cleaning box are arranged on the outer wall of the top of the bottom plate, and a sliding chute is formedin the inner wall of the bottom of the cleaning box; and the inner wall of the sliding chute is slidably connected with a waste liquid box through a sliding block, and the outer wall of one side of the waste liquid box and the inner wall of one side of the cleaning box are provided with the same fourth hydraulic rod. Insert holes are formed in the outer walls of the tops of brush rods, each cleaning brush is of a three-quarter circle structure, each test tube rack is of a transverse Z-shaped structure, the vacant quarter of each cleaning brush can just pass through the corresponding test tuberack, the length of a second hydraulic rod is adjusted so that the cleaning brushes can move up and down to effectively clean the test tubes, and liquid spray showers can spray cleaning water and sterilizing water to effectively clean the test tubes.

The invention belongs to the technical field of culture devices, and in particular relates to a constant temperature cell culture device for biotechnology research and development. The constant temperature cell culture device aims at solving the problem that the existing culture device is simple in structure, single in function and poor in culture effect. The culture device comprises a base. The bottom outer wall of the base is provided with a groove, and sliding rails are fixed on the inner walls on two sides of the groove through screws. The inner walls of the sliding rails are in sliding connection with sliding blocks, a horizontally arranged mounting plate is welded on the outer walls of the opposite sides of the two sliding blocks, universal wheels are fixed at four corners of the bottom outer wall of the mounting plate through screws, and a sterilization box is fixed on the top outer wall of the base through screws. The culture device achieves good culture effect and high intelligence degree, significantly improves the culture efficiency of cells, ensures the culture quality of the cells, greatly reduces the culture time, significantly prolongs the service life and ensures culture of the cells under aseptic condition.

The invention discloses a method for analyzing a regulation effect of deer skin protein peptide on macrophage immune cells, and belongs to the technical field of biotechnology research, development and analysis. By compared with proliferation activities of samples with different concentrations, a result shows that the proliferation index of RAW264.7 cells is increased along with the increase of adeer skin protein peptide concentration, and the concentration is 200[mu]g / mL and is closest to an LPS positive control group; in order to verify the influence of deer skin peptide on immunoregulation, the influence of deer skin protein peptide with different mass concentrations on NO secretion amount, LZM activity and release of cytokines such as IL-6 and TNF-[alpha] is researched, and the resultshows that the NO secretion amount is increased along with the increase of the sample concentration; after the deer skin protein peptide acts for 24 hours, the secretion function of RAW264.7 cytokines is obviously increased, and when the concentration is 50 [mu]g / mL, the levels of all the cytokines are close to those of an LPS group, which indicates that the deer skin protein peptide has the effect of enhancing cytokine secretion; in conclusion, the deer skin protein peptide has a potential effect of enhancing the immunocompetence, and can provide reference for subsequent research.

In the present invention, a biological reagent is fixed on the first supporting body and the biological reagent fixed on the first supporting body is contacted with biological molecule fixed on the second supporting body when the dot array is used to detect the biological molecule fixed on the second supporting body. At least one biological reagent is combined with biological molecule on the second supporting body, and then the first supporting body is separated from the second one as at least one biological reagent is separated from the first supporting body to combine on the second supporting body.

The invention belongs to the field of biotechnology research, and particularly relates to a TEVP (Tobacco Etch Virus Protease) active inclusion body as well as a preparation method thereof and application thereof in the preparation of a recombinant protein. The TEVP active inclusion body consists of an ELK16 self-polymeric peptide and TEVP that are separated by two PT connectors. The preparation method of the TEVP active inclusion body comprises the steps of an expression vector construction, an active inclusion body expression and purification, recombinant protein cleavage and target protein recovery. Compared with existing TEVP, the TEVP active inclusion body proviced by the invention has the advantages of simple preparation, easiness for being removed from an end product, low cost and the like, and can be applied to prepare various recombinant proteins.

The invention belongs to the field of biotechnological research, and particularly relates to long-acting interferon and a preparation method thereof. The long-acting interferon is composed of interferon and streptococcal G protein C2 structural domain, and the preparation method includes the steps of fusion expression vector, fusion interferon expression and purification, purification tag removal and fusion interferon recovery. Compared with the existing recombinant interferon, the long-acting interferon has the advantages of being simple in preparation process, low in cost, long in half-life period and the like, and can be used for the preparation of long-acting interferon for human and animals.

The invention discloses an instrument cleaning device capable of drying conveniently for biotechnology research and development. The instrument cleaning device comprises a supporting base, wherein anair cylinder is connected to the outer wall of one side of the top of the supporting base through bolts, and a telescopic rod is connected to the inner wall of the top of the air cylinder in an inserting mode; a top plate is welded to the outer wall of the top of the telescopic rod, and a water inlet pipe is welded to the outer wall of one side of the telescopic rod; a supporting column is weldedto the outer wall of the top of the supporting base, and an electric sliding track is welded to the outer wall of the top of the supporting column; an electric sliding block is slidably connected to the outer wall of the top of the electric sliding track, a storage block is welded to the outer wall of the top of the electric sliding block, and a storage groove is formed in the outer wall of the top of the storage block. According to the instrument cleaning device capable of drying conveniently for biotechnology research and development, a plurality of devices can be cleaned at the same time, speeding up the cleaning speed is facilitated, manual cleaning is not required, the devices after being cleaned can be dried conveniently, the subsequent operation affected by water attaching on the devices is avoided, a large amount of time is saved, and the working efficiency of the instrument cleaning device is improved.

The invention belongs to the field of biotechnology research and in particular relates to self-polymerized peptide fused CD151 protein as well as a preparation method and application thereof. The self-polymerized peptide fused CD151 protein is composed of pig CD151 protein PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) combined segment and an ELK16 self-polymerized peptide. The preparation method comprises the following steps: constructing an expression vector, expressing and purifying fused protein, determining a PRRSV combined segment and concentrating, purifying and detecting viruses. The self-polymerized peptide fused CD151 protein can be used for concentrating and purifying PRRSV and monitoring the environment. Compared with the other methods, the self-polymerized peptide fused CD151 protein has the advantages of being sensitive, simple, economical and the like when being used for the concentration, purification and PRRSV detection, can be used for concentrating and purifying the PRRSV of tissue samples and can also be used for PRRSV detection and separate culture of environment samples.

The invention belongs to the bio-technical field, and particularly relates to an air purifying device with a humidifying function based on the biological technology. In order to solve the problems that the air purifying device is complex in structure, is poor in air purifying effect, is single in function, and cannot meet requirements very well, the invention discloses the scheme that the air purifying device comprises a bottom box, wherein the four corners of the outer wall of the bottom of the bottom box are connected with universal wheels through bolts; and the outer wall of one side of thebottom box is connected with two connecting frames which are symmetrically arranged through bolts. The air purifying device realizes a humidifying function, effectively improves the quality of air inside a biotechnical research room, effectively improves working efficiency of a biotechnical worker, conveniently regulates the direction of a gas inlet of a gas inlet tube according to a practical condition in a laboratory by a user, effectively improves use convenience of the device, conveniently replaces consumables such as a purifying agent, a toner cartridge and a drying agent inside the boxbody frequently by the user, and ensures air purifying efficiency of the device.

The invention discloses a sealed fermentation device for biotechnology research and development. The sealed fermentation device comprises a box body, wherein a material guiding table is fixed to the center of the inner wall of the top of the box body, motors are fixed to the two sides of the outer wall of the bottom of the box body, the top ends of output shafts of the motors penetrate through theinner wall of the box body, stirring mechanisms are fixed, and a sleeve is fixed to the center of the outer wall of the top of the box body; A piston rod is inserted into the sleeve, an extrusion mechanism is fixed to the bottom end of the piston rod, a reset mechanism is fixed to the top end of the piston rod, and an air storage mechanism fixedly communicates between one side of the sleeve and the box body. The solid waste is extruded by utilizing air pressure power generated by air, so that the fermentation completeness is improved.

The invention discloses a multifunctional workbench for biotechnology research and development, and relates to the technical field of biotechnology research and development equipment. The device comprises a research and development workbench, a driving cavity is formed in the upper end in the research and development workbench, a storage driving motor is fixed in the driving cavity, a first bevel gear is fixed to the output end of the storage driving motor, a second bevel gear is assembled to the lower end of the first bevel gear, and a threaded rod is welded to the interior of the second bevel gear. Through mutual cooperation of a storage driving motor, a threaded rod and a classified storage mechanism, tools and reagents for research and development can be stored in a classified mode through the device, the workbench is clean and tidy, research and development personnel can conveniently take the tools and reagents needing to be used, the device is convenient to use, and the working efficiency is improved. And through mutual cooperation of the research and development workbench, hollow supporting legs, an H-shaped fixing plate and a lifting mechanism, the height of the device can be adjusted according to the height of research and development personnel, and the device is convenient to use.

The invention discloses a cell culture device for biotechnology research, and the cell culture device comprises a processing tank body; a plurality of supporting bottom feet are fixedly mounted at the bottom end of the processing tank body, the bottom ends of the supporting bottom feet are supported on the ground, and a processing cavity is arranged in the processing tank body; a tank body cover plate is fixedly connected into an opening in the top end of the machining cavity through a first thread locking structure, and a driving motor is fixedly connected to the outer wall of the top end of the tank body cover plate. According to the invention, by utilizing the arranged processing cavity, especially a transmission rod and a stirring rod arranged in the processing cavity, after the whole stirring rod is driven to rotate through the operation of the driving motor, cell culture materials entering the processing cavity are fully crushed and then are guided into corresponding culture drawers in batches; then the materials are guided into the corresponding culture drawers in batches, different culture materials can be researched separately, and the whole structure is reasonable in design, easy to operate, high in mechanization degree and worthy of later popularization and application.

The invention discloses a method for analyzing immunoregulation of deer fetus oligopeptides on macrophages, and belongs to the technical field of biotechnology research, development and analysis. After screening, the proliferation index of RAW264.7 cells is significantly higher than that of a blank group, and is closest to that of an LPS positive control group. The method analyzes the influence ofdifferent mass concentrations of deer fetus oligopeptides on the phagocytic function, the NO secretion amount and the release of cytokines such as IL-1 beta, IL-6 and TNF-alpha; and the obtained results show that, under the concentration of 25 mu g / mL, the NO has the maximum release amount and is closest to the LPS group. According to the method, cell cycle inspection is carried out simultaneously; data results prove that with the increase of the mass concentration of the deer fetus oligopeptides, the cell proportion in the G0 / G1 stage is in a trend of firstly rising and then falling, the cell proportion in the S stage and the G2 / M stage is in a trend of firstly falling and then rising, and the proportion of the cells in each stage is closest to that of the LPS group at the concentrationof 25 mu g / mL; the G0 / G1 stage is the synthesis period of RNA and protein, so that the expression quantity of related protein is increased, and the secretion amount of phagocytic action, NO and cytokines reaches the maximum value in the period.