255 results about "High specific activity" patented technology

Antimicrobial peptides enable an alternate approach to developing antimicrobial coatings due to their targeting of the membranes of the bacteria. High specific activity is achieved by orienting the peptides so that the antimicrobial ends of the peptides maximally contact the bacteria. In one embodiment, one end of the peptide is covalently attached directly to the substrate. In another embodiment, the peptides are immobilized on the substrate using a coupling agent or tether. Non-covalent methods include coating the peptide onto the substrate or physiochemically immobilizing the peptides on the substrate using highly specific interactions, such as the biotin / avidin or streptavidin system. The compositions are substantially non-leaching, antifouling, and non-hemolytic. The immobilized peptides retain sufficient flexibility and mobility to interact with and de endocytosed by the bacteria, viruses, and / or fungi upon exposure. Immobilizing the peptides to the substrate reduces concerns regarding toxicity of the peptides and the development of antimicrobial resistance, while presenting substantially all of the peptide at the site of action at the surface of the substrate.

The invention discloses core / shell type catalyst particles comprising a Mcore / Mshell structure with Mcore=inner particle core and Mshell=outer particle shell, wherein the medium diameter of the catalyst particle (dcore+shell) is in the range of 20 to 100 nm, preferably in the range of 20 to 50 nm. The thickness of the outer shell (tshell) is about 5 to 20% of the diameter of the inner particle core of said catalyst particle, preferably comprising at least 3 atomic layers. The core / shell type catalyst particles, particularly the particles comprising a Pt-based shell, reveal a high specific activity. The catalyst particles are preferably supported on suitable support materials such as carbon black and are used as electrocatalysts for fuel cells.

The present invention provides one kind of improved high specific activity phytase and its coding gene, and the improved phytase has even higher heat stability. The present invention also provides recombinant yeast cell containing the phytase gene of the present invention. The recombinant yeast of the present invention has phytase expressing amount up to 6.5E6 U in each ml of fermented liquid. The phytase of the present invention may be used widely in feed and food industry.

The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.

The invention discloses core / shell type catalyst particles comprising a Mcore / Mshell structure with Mcore=inner particle core and Mshell=outer particle shell, wherein the medium diameter of the catalyst particle (dcore+shell) is ≧20 nm. The thickness of the outer shell (tshell) comprises at least 3 atomic layers.The core / shell type catalyst particles, particularly the particles comprising a Pt-based shell, reveal a high specific activity. The catalyst particles are preferably supported on suitable support materials such as carbon black and are used as electrocatalysts for fuel cells.

Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and / or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.

The invention provides a three-dimensional hollow material, a preparation method thereof, and an application thereof in electrochemical energy storage devices. The three-dimensional hollow material is a self-supporting three-dimensional hollow material obtained through high-temperature carbonization of a foam resin material containing an ether bond under normal-temperature oxygen-free inert atmosphere conditions. The three-dimensional hollow material can be used as an electrode material to form a three-dimensional hollow carbon-based electrode; and the three-dimensional hollow material also can be compounded with a high-specific activity active electrode material to form a composite electrode. The three-dimensional hollow carbon-based electrode and the composite electrode can be applied to the electrochemical energy storage devices. The prepared three-dimensional structure and hollow structure coupled flexible three-dimensional hollow material can be used as an electrochemical energy storage device electrode or current collector. For example, the three-dimensional hollow material can solve the instability problem during intercalation / deintercalation of large ions (such as Na<+> and K<+>) and obtain a high specific capacity and a long life when used as a sodium and potassium ion battery negative electrode.

Compositions of high specific activity 117mSn with specific activity of greater than 100 Ci / g Sn and methods of producing the same. The method includes exposing 116Cd to an α-particle beam of sufficient incident kinetic energy and duration to convert a portion of the 116Cd to 117mSn to form an irradiated material. The irradiated material is dissolved to form an intermediate solution containing 117mSn and 116Cd. The 117mSn is separated from the 116Cd to yield high specific activity 117mSn.

The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.

A method for treating bacterial infections in a patient is provided. The method includes the step of administering a therapeutically effective amount of a single naturally-occurring or engineered bacteriocin, or combinations thereof, designed to have high specific activity against the bacterial infection to the patient so that a rate of resistance to the bacteriocin in the patient is decreased. The colicins and other bacteriocins or combinations of colicins and other bacteriocins target and kill specific bacterial pathogens in a manner that results in a high specific killing activity and decreased incidence of pathogen resistance. To this end, the characteristics of existing colicins and other bacteriocins are modified in order to enhance and amplify their therapeutic value.

The invention relates to the genetic engineering field, especially to a strain Bispora sp.MEY-1 which produces an acidophilic beta-mannanase and the acidophilic beta-mannanase MAN5A got from the strain having an amino acid sequence shown as SEQ ID NO.1 or 2, a gene man5A for coding said beta-mannanase having a nucleotide sequence shown as SEQ ID NO.1 or 2, and a recombinant vector containing said gene and applications. The beta-mannanase of the invention has the following advantages: the optimum pH 1.0-1.5, the optimum temperature 65 DEG C, good pH stability and thermostability, high specific activity, good protease resistance and easy for industrial fermentation production. The product of the invention can be widely used for the animal feeding-stuffs, the food, the medicament, the brewing and the energy industry as a novel enzyme preparation.

A new process, particularly simple and economical, for FSH and LH separation and purification starting from crude HMG preferably urinary, comprising the following steps: 1) optional exhaustion of crude HMG viral charge in aqueous EtOH 2) ion-exchange chromatography on weakly basic anionic resins of DEAE type; 3) affinity chromatography on resin having an antraquinone derivative as a ligand; 4) optional ion-exchange chromatography on strongly basic anionic resins; Hormones obtained thereby, in particularly pure form and having high specific activity, may subsequently undergo a depyrogenation step.

The invention discloses a pullulan enzymatic mutant with high specific activity and high thermal stability and a preparation method of the pullulan enzymatic mutant, and belongs to the field of genetic engineering and enzyme engineering. The invention improves the specific activity and the thermal stability of pullulan enzyme by site-specific mutagenesis, and provides a mutation scheme through which the catalytic specific activity and the thermal stability of the pullulan enzyme derived from debranch bacillus are improved. At least one property of the pullulan enzymatic mutant is changed: (1) the optimal reaction temperature is increased, (2), the thermal stability on the condition that the pH is 4.0 to 5.0 is improved; and (3) the specific activity on the condition that the pH is 4.0 to 5.0 is improved. The pullulan enzymatic mutants are more suitable for the production process of starch saccharification as compared with wild type pullulan enzyme.

Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and / or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.

The invention relates to a device and a method for measuring strong gamma ray energy spectrum using a scattering method. The device for measuring strong gamma ray energy spectrum using the scattering method comprises an illuminator with a shield function, a high specific activity isotope radioactive source arranged in the illuminator, a front collimator used for constraining a gamma ray emerged through a collimating aperture of the illuminator, a scattering target used for receiving a quasi-parallel gamma ray beam formed by the collimation of the front collimator and a detector used for measuring a scattering ray with 662 kilo electron volt ( keV ) average energy obtained by scattering in a 50 degrees angle, wherein the detector is arranged in an inner cavity of a shield body with the collimating aperture. The device and the method for measuring strong gamma ray energy spectrum using the scattering method resolve the difficult problem of measurement of a strong gamma ray generated by a cobalt-60-source in a calibration laboratory.

Electrocatalyst durability has been recently recognized as one of the most important issues that have to be addressed before the commercialization of the proton exchange membrane fuel cells (PEMFCs). The present invention is directed to a new class of cathode catalysts based on supportless platinum nanotubes (PtNTs) and platinum alloy nanotubes, for example, platinum-palladium nanotubes (PtPdNTs), that have remarkable durability and high catalytic activity. Due to their unique combination of dimensions at multiple length scales, the platinum nanotubes of the present invention can provide high platinum surface area due to their nanometer-sized wall thickness, and have the potential to eliminate or alleviate most of the degradation pathways of the commercial carbon supported platinum catalyst (Pt / C) and unsupported platinum-black (PtB) as a result of their micrometer-sized length. The platinum nanotube catalysts of the present invention asymptotically approach a maximum of about twenty percent platinum surface area loss in durability test, while the commercial PtB and Pt / C catalysts lose about fifty-one percent and ninety percent of their initial surface area, respectively. Moreover, the PtNT and PtPdNT catalysts of the present invention show higher mass activity and much higher specific activity than commercial Pt / C and PtB catalysts.

The invention provides a kind of broad-spectrum embedded acid enzyme and its coding gene. The invention also provides the expressing carrier and the recomposing yeast cell which contains the carrier. In the invention, the expressing quantity of embedded acid enzyme in recomposing yeast reaches to 520000U / ml ferment liquor.

Disclosed are improved methods for the synthesis of phospholipid ether analogs and alkyl phosphocholine analogs. The methods allow greater versatility of the reactants used and greater ease in synthesizing alkyl chains of varying length while affording reaction temperatures at room temperature or below. The methods disclosed herein provide reactants and conditions using alkyl halides and organozinc reagents and do not utilize Gringard reactions thus, allowing greater ease of their separation and purity of products. The PLE compounds synthesized by the methods disclosed herein can also be used for synthesizing high specific activity phospholipid ether (PLE) analogs, for use in treatment and diagnosis of cancer.

The invention discloses a pullulanase mutant having a high specific activity and a high thermal stability, and a preparation method thereof. The mutant comprises one, two or three substituents relative to debranching Bacillus spp Pullulanase active amino acid residues and related with the thermal stability of pullulanase; and the active amino acid residues comprise 503th aspartic acid, and can be mutated to form arginine, phenylalanine and tryptophan or tyrosine, the above mutation improves the high specific activity and the high thermal stability of the pullulanase, and at least one of the cases comprising the rise of a best reaction temperature, the improvement of the thermal stability at a pH value of 4.0-5.0 and the improvement of the specific activity at a pH value of 4.0-5.0 is realized. The mutant is more suitable for the starch saccharification production process than wild pullulanase.

The invention provides a heat-resistant beta-mannanase and its coding gene, recombinant bacterium and use. The heat-resistant beta-mannanase as a protein is shown in the formula (a) or (b). The heat-resistant beta-mannanase shown in the formula (a) comprises the amino acid sequence 1 of the sequence table; and the heat-resistant beta-mannanase shown in the formula (b) is a protein derived from the amino acid sequence 1 though replacement and / or deletion and / or addition of multiple amino acid residues and having a beta-mannanase activity. The invention also provides the coding gene of the heat-resistant beta-mannanase and the recombinant bacterium which is engineering bacterium and contains the coding gene. The recombinant bacterium is recombinant pichia pastoris. The heat-resistant beta-mannanase has a high specific activity, good acid stability and a wide catalysis pH range, and is suitable for being used as an additive of monogastric animals such as pigs and chickens.

Disclosed are improved methods for the synthesis of phospholipid ether analogs and alkyl phosphocholine analogs. The methods allow greater versatility of the reactants used and greater ease in synthesizing alkyl chains of varying length while affording reaction temperatures at room temperature or below. The methods disclosed herein provide reactants and conditions using alkyl halides and organozinc reagents and do not utilize Gringard reactions thus, allowing greater ease of their separation and purity of products. The PLE compounds synthesized by the methods disclosed herein can also be used for synthesizing high specific activity phospholipid ether (PLE) analogs, for use in treatment and diagnosis of cancer.

Compositions of high specific activity 117mSn with specific activity of greater than 100 Ci / g Sn and methods of producing the same. The method includes exposing 116Cd to an α-particle beam of sufficient incident kinetic energy and duration to convert a portion of the 116Cd to 117mSn to form an irradiated material. The irradiated material is dissolved to form an intermediate solution containing 117mSn and 116Cd. The 117mSn is separated from the 116Cd to yield high specific activity 117mSn.

The present invention relates to alkaline proteases having high specific activity and strong oxidant resistance. The present invention also relates to alkaline proteases having excellent detergency that are to be added to a detergent.

Gamma radiation imaging system for non-destructive inspection of the luggage contains a DR sub-system, which obtains the projected image of the luggage by translationally scanning, and a CT sub-system, which obtains the tornograph image of the luggage by rotating scanning. Said DR sub-system includes a fixed gantry, a conveyor system, a radiation source mounted on the fixed gantry, front and back collimators, and the array detector. Said CT sub-system includes a rotating gantry, a conveyor system, a radiation source mounted on the rotating gantry, front and back collimators, and the array detector, wherein, the radiation source in said CT sub-system is 192Ir or 75Se radioactive isotope gamma source with high specific activity. Said gamma source is enclosed in a shield chamber with a projecting opening. The shield chamber is mounted on said rotating gantry. Said array detector is adapted for detecting gamma ray of 192Ir or 75Se radioactive isotope; The radiation source in said DR sub-system is 192Ir or 75Se radioactive isotope gamma source with high specific activity, said gamma source is enclosed in a shield chamber with a projecting opening. The shield chamber is mounted on said fixed gantry. Said array detector is adapted for detecting gamma ray of 192Ir or 75Se radioactive isotope.

The present invention provides novel nucleic acid labeling techniques that generate nucleic acid probes with specific activities at least ten fold higher than the levels obtained using standard labeling methods. Specifically, the methods of the invention provides methods of producing nucleic acid probes that each comprises multiple labeled nucleotides. The methods can be used to generate RNA, DNA or hybrid probes. The invention also provides reaction mixtures and kits for the practice of the methods of the invention and compositions comprising the probes generated according to the methods of the invention.

The invention provides an analytical method of correlation of xylanase heat resistance and an N-terminal disulfide bond, and belongs to the technical field of microbial genetic engineering. A result that the N-terminal disulfide bond is one of factors which affect the xylanase heat resistance is proved by adopting a scheme of combining three kinds of bioinformatics methods including the homologous comparison, the homologous modeling and the molecular dynamics simulation of a primary structure of the zymoprotein, and the result is also proved by combining an experimental means of site-specific mutagenesis and by analyzing a heat resistance mechanism of the EvXyn11TS. The research result establishes a solid foundation for the heat resistance transformation of the 11 family normal temperature high specific activity xylanase which has a similar primary structure with the EvXyn11TS.

The present invention provides a recombined-batroxobin, having following characteristics: (a) the molecular weight is 29-32Kda; (b) at least 90% batroxobin in the recombined-batroxobin has correct 6 pairs of disulfide bonding; Cys7-Cys139, Cys26-Cys42, Cys74-Cys230, Cys118-Cys184, Cys150-Cys163 and Cys174-Cys199; (c) the 146 position and the 225 position in the SEQ ID NO:1 are suffered from glycosylation; (d) the specific activity of the batroxobin is more than 1500KU / mg.