117 results about "Tetrasaccharide" patented technology

The invention discloses an enzymolysis-HPLC method for detecting enoxaparin. The method comprises steps of: a. complete enzymolysis of enoxaparin: adding a mixed enzyme solution with enzyme I, enzyme II and enzyme III in a ratio of 8:1:2 to an enoxaparin solution with a concentration of 10-200mg/ml and carrying out an enzymolysis at room temperature for 48 h. b. HPLC analysis: carrying out a SAX-HPLC analysis on the degradation products; c. calculation of variety and content of disaccharide and tetrose units: determining variety and content of disaccharide and tetrose units according to a wash out time of prior standard disaccharide and tetrose and calculating percentage content of each of the eight disaccharides and one tetrasaccharide.

The invention discloses a preparation method for disaccharide, tetrasccharide and hexaose of chondroitin sulfuric acid, and belongs to the technical field of food. The method comprises the following steps that: the chondroitin sulfuric acid adopted as a raw material is degraded by hyaluronidase, so as to obtain chondroitin sulfuric acid oligosaccharide; through filtering separation by G-25 gel, desalination by G-10, DEAE cellulose 52 anion exchange chromatographic column separation, natural preparation of gel electrophoretic separation, and the like, the disaccharide, tetrasccharide and hexaose of the chondroitin sulfuric acid can be prepared. The invention relates to the preparation method for the disaccharide, the tetrasccharide and the hexaose of the chondroitin sulfuric acid. According to the invention, simultaneous preparation of three types of chondroitin sulfuric acid oligosaccharide can be firstly achieved, and a technical base for industrial preparation for single degree of polymerization chondroitin sulfuric acid oligosaccharide can be provided.

A liquid pharmaceutical composition is contemplated that comprises a pharmaceutically effective amount of a drug dissolved or dispersed in an aqueous medium. The aqueous medium consists essentially of water, about 3% to about 10% w/v polyvinylpyrrolidone, about 60% to about 75% w/v of C₃-C₆ polyol that includes more than 55% w/v of a non-reducing disaccharide, trisaccharide or tetrasaccharide such as sucrose, optionally about 0.01% to about 0.5% w/v of a glycyrrhetic acid, glycyrrhizinate derivative or salt thereof, and one or more flavorants, and preferably includes one or more preservatives. The liquid composition is room temperature stable, and may have a pleasant taste.

The invention discloses a method for separating hyaluronate tetrasaccharide from hyaluronate hexasaccharide, and belongs to the technical field of bioengineering. The method comprises the following steps: adopting Q Sepharose fast flow as an ion exchanger, Tris-HCl with the pH value of 8-9 as an equilibrium liquid, and 0.125 M NaCl with the pH value of 8, performing linear gradient elution to separate hyaluronate tetrasaccharide from hyaluronate hexasaccharide, and desalting to obtain pure products of hyaluronate tetrasaccharide and hyaluronate hexasaccharide.

The invention discloses an ultralow molecular weight hyaluronic acid and a preparation method thereof, and belongs to the technical field of biochemical engineering. Macromolecular hyaluronic acid used as a raw material undergoes production processes of hyaluronidase hydrolysis, heating inactivation, activated carbon filtration, spray drying and the like to obtain the ultralow molecular weight hyaluronic acid product with the average molecular weight lower than 1200 Daltons. The ultralow molecular weight hyaluronic acid is a mixture of hyaluronic acid disaccharide to dodecaose, the content ofthe hyaluronic acid disaccharide is 5-40 %, the content of the hyaluronic acid tetrasaccharide is 40-70 %, the content of the hyaluronic acid hexasaccharide is 10 to 30 %, the content of the hyaluronic acid octasaccharide is 1-15%, the content of the hyaluronic acid decaose is 1-10%, and the content of hyaluronic acid dodecaose or above is lower than 6%. Compared with common commercially availablelow-molecular hyaluronic acid, the product of the invention has remarkable permeation moisturizing and repair promoting capacity and can be widely applied to the fields of medical products, health care products, cosmetics and the like. The method is simple and convenient to operate, mild in conditions, free of organic solvents, high in enzymolysis efficiency and suitable for large-scale industrial production.

The invention discloses a synthetic method of synthesizing double-sialylated tetrasaccharide by a chemically controllable enzyme method. The invention further provides two intermediates, which are used for synthesizing and preparing the double-sialylated tetrasaccharide, are compounds respectively as shown in formulas IV and V. According to the synthetic method disclosed by the invention, the flexibility of a chemical synthetic method and the high-area selectivity and high efficiency of the enzyme synthetic method are combined together, so that synthesis of the double-sialylated tetrasaccharide by using a chemical intervention method is firstly realized, defects such as low substrate reaction activity, multiple synthetic steps and low yield facing in existing chemical synthesis of the double-sialylated tetrasaccharide are solved, as well as a problem that sialyl transferase is difficult to obtain and only glycopeptides is identified in the enzyme method is solved; the synthetic method disclosed by the invention has the advantages of high substrate reaction activity and high yield, and therefore, the synthetic method has important significance in researching biological functions of sialic acid glucoside on molecular level and developing carbohydrate drugs based on the biological functions.

The invention relates to a preparation method of a chondroitin sulfate D tetrasaccharide, and belongs to the technical field of biochemical engineering. According to the preparation method, the chondroitin sulfate D tetrasaccharide is prepared and obtained through steps of ethanol precipitation, anion exchange chromatographic separation, gel chromatographic separation and the like by using shark chondroitin sulfate as a raw material and adopting chondroitin sulfate AC enzyme degradation. The preparation method has the beneficial effects that the chondroitin sulfate D tetrasaccharide with definite structure and molecular weight can be prepared and obtained; the yield is higher, and the probability is provided for the research of a chondroitin sulfate oligosaccharide monomer.

The invention discloses a pseudoalteromonas WZU4771, the strain is preserved in China General Microbiological Culture Collection Center, and preservation number is CGMCCNO. 3407. The kappa-carrageenan hydrolase produced by the pseudoalteromonas WZU4771 can hydrolyze kappa-carrageenan, and finally kappa-new cara tetrasaccharide sulphate is obtained.

The invention provides a preparation method of superhigh malt syrup of which the maltose content is higher than 98%. The preparation method mainly comprises the following steps: preparing malt syrup, carrying out chromatographic separation, carrying out yeast fermentation to remove glucose, and separating yeast cells with a ceramic membrane to obtain the superhigh malt syrup in which the maltose content on dry basis is higher than 98%. The invention adopts the conventional chromatographic separation resin, thereby avoiding the steps of resin pretreatment, transformation and the like, and being efficient and quick; the yeast rapid fermentation is adopted to remove glucose, and thus, the method has the advantages of fewer byproducts and mild reaction conditions and is convenient to operate; the maltose content in the obtained product is higher than 98%; and meanwhile, maltotriose, maltotetrose, glucan tetrasaccharide above, and other miscellaneous glucoses are removed.

The object of the present invention is to provide a lipid-regulating agent or a composition for regulating the amount of lipids comprising the agent. The present invention solves the above object by providing a lipid-regulating agent comprising a cyclic tetrasaccharide and/or its saccharide-derivative(s) and a composition for regulating the amount of lipids comprising the lipid-regulating agent.

The invention discloses a preparation method of galacto-mannan-oligosaccharides and application of the galacto-mannan-oligosaccharides. The preparation method comprises the following steps: (1) performing segmented composite enzymatic hydrolysis on galacto-mannan-oligosaccharides such as guar gum, sesbania gum, locust bean gum, fenugreek gum and Tara gum, wherein the enzyme is a complex enzyme ofacid mannase and cellulase; and (2) by segmented ultrafiltration classification, obtaining degraded galacto-mannan-oligosaccharides which are a mixture of a disaccharide, a trisaccharide, a tetrasccharide and a pentosaccharide. The preparation process is simple and easy, and is environmentally friendly. The conversion rate of the galacto-mannan-oligosaccharides is high, and the galacto-mannan-oligosaccharides are easy to separate. The prepared galacto-mannan-oligosaccharides can be used as prebiotics to be applied to food and animal feed, the proliferation of a butyric acid-production probiotic is promoted, and the intestinal environment is improved.

For extending the uses of a compound represented by, Chemical formula 1, the object of the present invention is to provide a derivative of cyclic tetrasaccharide whose physicochemical properties are changed from those of cyclic tetrasaccaride, a composition comprising the same, and a process for producing the same. The present invention solves the above object by providing a derivative of cyclic tetrasaccharide, which is produced by the steps of reacting a compound represented by Chemical formula 1 with a reactive reagent and substituting one ore more hydroxyl groups with substituents except hydroxyl group and O-glycosyl group; a composition comprising the same; and a process for producing the derivative of cyclic tetrasaccharide. Chemical formula 1

The invention discloses a method for preparing kestose and nystose through yacon. Method of water extraction by alcohol sedimentation is adopted and supernate thereof is taken, concentrated, frozen and dried to obtain faint yellow yacon oligosaccharide. The yacon oligosaccharide is analyzed through high pressure liquid chromatography with a chromatography condition as follows: a chromatographic column is APS-2HYPERSIL, a column temperature is 30 DEG C, a moving phase is acetonitrile-water (75 to 25, V/V), a flow velocity is 0.8ml/min, a sample size is 20 Mul and a differential detector is arranged. The yacon oligosaccharide comprises fructose, glucose, saccharose, kestose, nystose and 1F-Fructofuranosyl nystose with contents respectively as 38,30 percent, 16.44 percent, 14.58 percent, 12.29 percent, 12.17 percent and 6.2 percent. Silicagel column chromatography is implemented on a prepared oligosaccharide sample; glacial acetic acid, chloroform, absolute ethyl alcohol and water with a proportion of 3 to 11 to 11 to 1 are used as an eluant for elution; the eluant is collected in sequence and then is concentrated, frozen and dried to obtain the kestose and nystose. The purity of the kestose and nystose prepared through high pressure liquid chromatography can be more than 90 percent.

The invention discloses an infant nutrition composition containing human milk oligosaccharide, and belongs to the technical field of infant nourishment processing. The human milk oligosaccharide consists of neutral human milk oligosaccharide and acidic human milk oligosaccharide, wherein the weight ratio of the neutral human milk oligosaccharide to the acidic human milk oligosaccharide is (60-70):(30-40); the neutral human milk oligosaccharide comprises one or more selected from 2'-fucosyl lactose (2'-FL), 3-fucosyl lactose (3-FL) and lactose-N-neotetraose (LNnT); and the acidic human milk oligosaccharide comprises one or more selected from 3'-sialyl lactose (3'-SL), 6'-sialyl lactose (6'-SL) and sialic acid. The composition provided by the invention contains human milk oligosaccharide andis closer to breast milk, so that the effects of proliferating probiotics, improving intestinal flora balance and promoting intestinal health can be improved.

The invention discloses recombinant escherichia coli for synthesizing lactoyl N-neotetraose and a construction method and application of the recombinant escherichia coli. The recombinant escherichia coli are obtained by knocking out a beta-galactosidase lacZ gene, a glucosamine-6-phosphodeaminase nagB gene, a UDP-acetylglucosamine epitope isomerase wecB gene and a UDP-glucose dehydrogenase ugd gene in the host genome of escherichia coli and overexpressing a lactose transportase lacY gene, beta-1, 3-N-glucosaminidase lgtA gene and a beta-1, 4 galactosyltransferase lgtB gene on the genome. The yield of lactoyl-N-neotetraose synthesized by recombinant escherichia coli reaches 985 mg/L, and a foundation is laid for further metabolic engineering transformation of escherichia coli to produce lactoyl-N-neotetraose.

The invention discloses a method for synthesis of chondroitin sulfate tetrasaccharide. The method comprises that orderly through enzymatic hydrolysis, protecting group protection, protecting group conversion, selective deprotection, hydroxyl oxidation and selective reduction, an intermediate compound shown in the formula O is prepared from hyaluronate as a starting material, and chondroitin sulfate tetrasaccharide is prepared from the intermediate compound shown in the formula O, wherein substituents in the formula O are specifically defined in the specification. The invention provides the intermediate compound for synthesis of chondroitin sulfate tetrasaccharide. Compared with the existing synthesis method, the method provided by the invention has the advantages of simple synthesis route, no glycosylation reaction, high reaction yield and good application prospect.

The invention discloses an amino acid sequence and a nucleotide sequence of a heat-resisting alkali-resisting and salt stable inulase exonuclease, and construction and application of an inulase exonuclease recombinant expression vector. Results of enzymatic property research show that the optimum reaction conditions of the inulase exonuclease are as below: pH 7.0, 50 DEG C and 10% NaCl. The enzyme can catalyze the hydrolysis of inulase, levan, sucrose, raffinose, kestose, nystose and Kestopentaose. In a catalysis process using inulase as a substrate, only generation of fructose is detected rather than oligosaccharide or other carbohydrates. The inulase exonuclease provided by the invention can be applied to the field of food industry and biological energy source as a novel biocatalyst for fructose production.

The invention discloses a human blood group antigen P1 pentasaccharide synthesis method. The method includes steps: adopting a one-pot multienzyme system for coupling galactose to trisaccharide as shown in a formula (III) through a beta1-4 glucosidic bond to synthesize tetrasaccharide as shown in a formula (IV); adopting the one-pot multienzyme system for coupling galactose to the tetrasaccharide as shown in the formula (IV) through an alpha1-4 glucosidic bond to synthesize pentasaccharide as shown in a formula (I), wherein in the formula (I), the formula (III) and the formula (IV), R1 refers to hydroxyl, azide substituted alkyl, alkynyl substituted alkyl, sulfydryl substituted alkyl, alpha- or beta- configuration substituted alkyl, alpha- or beta- configuration serine residue and alpha- or beta- configuration threonine residue. By integration of high regioselectivity and high efficiency of enzymatic synthesis, P1 antigen pentasaccharide is synthesized for the first time. Glycosyltransferase, glucose nucleoside generating enzymes and glucokinse adopted in the synthesis method are all derived from prokaryotes, and high protein expression quantity, high substrate adaptability and high catalytic efficiency are realized.

The present invention related to a saccharide-based cholera toxin detection sensor for detection of Vibrio cholerae and its use. More specifically, the present invention relates to a carbohydrate chip for detection of Vibrio cholerae, a method for detecting Vibrio cholerae using the same, and a method for preparing the same, where the carbohydrate chip comprises GM1 pentasaccharide, GM2 tetrasaccharide, asialo GM1 tetrasaccharide, GM3 trisaccharide, galactose-β 1,3-N-acetylgalactosamine, lactose, and sialic acid that are immobilized on the surface of a solid substrate.

The invention relates to a method for detecting target breast milk oligosaccharide in formula food. The target breast milk oligosaccharide may include or be one or more of 2 '-fucosyllactose, 3-fucosyllactose, 3'-sialyllactose, 6 '-sialyllactose, lactose-difucosyltetraose, lactose-N-neotetraose, and lactose-N-tetraose. The method comprises the following steps: removing fat and protein from a sample, reducing by adopting a reducing agent such as NaBH4, detecting by adopting liquid chromatography-mass spectrometry, and quantifying by adopting an external standard method. According to the method, the target breast milk oligosaccharide (HMO) can be simply, conveniently, quickly, accurately, qualitatively and quantitatively detected.

The invention discloses a production method of enoxaparin sodium, which comprises the following steps: carrying out enzymolysis on heparin sodium, carrying out HPLC (high performance liquid chromatography) analysis, calculating the contents of various peaks by a peak area normalization process, determining the varieties of disaccharides and tetrasaccharides of the heparin sodium subjected to enzymolysis according to a standard spectrum of disaccharides and tetrasaccharides after heparin sodium enzymolysis, comparing with the contents of disaccharides and tetrasaccharides of the standard spectrum, and selecting the satisfactory heparin sodium raw material to produce the enoxaparin sodium. The method is simple and effective, can ensure the safety and effectiveness of the product, controls the quality of the enoxaparin sodium from the source, can avoid the waste of the active pharmaceutical ingredient heparin sodium, greatly saves the cost and enhances the production efficiency.