35 results about "Proteus" patented technology

The invention belongs to the technical field of heavy metal soil pollution remediation, and particularly relates to a heavy metal tolerant bacterial strain and application thereof, the bacterial strain is named as Proteus mirabilis D2 and has been preserved in Guangdong Microbial Culture Collection Center on December 04, 2024, and the preservation number is GDMCC No: 65293. According to the invention, effective reduction of biotoxicity of culturable proteus mirabilis (P.mirabilis D2) with heavy metal tolerance and MICP functions on soil polluted by high cadmium, lead and zinc is realized, and corresponding parameters of technical application are determined.

The invention belongs to the technical field of bacteriophages, and discloses a proteus mirabilis bacteriophage PF05, a bacteriophage composition thereof and application of the proteus mirabilis bacteriophage PF05, the proteus mirabilis bacteriophage PF05 is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 22, 2024, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.9687. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The bacteriophage is resistant to high temperature, wide in lysis spectrum, high in storage stability and good in gastric acid tolerance, can keep activity in the stomach for a long time, and has a good prevention and treatment effect on infection of proteus mirabilis in the intestines and the stomach. The compound can be prepared into medicines, environment disinfectants, feeds, water additives and the like for preventing and treating proteus mirabilis for application, not only effectively prevents and treats propagation of proteus mirabilis, but also has the effects of reducing the death rate of chicken flocks, reducing digestive tract infection of the chicken flocks, avoiding diarrhea of the chicken flocks and improving the resistance of the chicken flocks, and has a very good market application prospect.

The invention provides a construction method and application of a Pseudomonas proteinans Pyo gene knockout strain, and belongs to the technical field of gene engineering. The invention provides a Pyo gene capable of influencing the virulence of pseudomonas proteus. The nucleotide sequence of the Pyo gene is shown as SEQ ID No.7. The invention also provides a preparation method of the Pyo gene capable of influencing the virulence of pseudomonas proteus. The invention provides a pseudomonas proteus Pyo gene knockout box, and then the gene knockout box is connected with a suicide plasmid to obtain a gene knockout vector. The invention further provides a homologous exchange method, and the gene knockout vector in the escherichia coli donor bacterium containing the gene knockout vector is transferred into the donor bacterium, so that the Pseudomonas proteinans Pyo gene knockout strain is obtained. Due to the deletion of the Pyo gene, the iron absorption and transportation capabilities of the pseudomonas proteinatica are reduced, the number of viable bacteria is reduced, and the pathogenicity of the pseudomonas proteinatica to the large yellow croaker is reduced.

The invention relates to the technical field related to medicines, in particular to a method for preventing and controlling orbiculaeas on the basis of an artemisia apiacea extract, which comprises the following specific steps: step 1, purchasing the artemisia apiacea extract from Shanghai Yuan Leaf Biotechnology Co., Ltd; step 2, separating blood egg whipstock in the economic crustacean animals to obtain an amoeboid-shaped trophozoite and microspores of the economic crustacean animals; 3, the amoeboid-shaped trophozoite is inoculated into a culture medium containing specific components to be cultured, the culture medium comprises 0.1% of mycillin, 5% of healthy portunus trituberculatus serum, 10% of fetal calf serum and 85% of NephropsSaline buffer solution, and the microspores are cultured with sterile filtered seawater. According to the method for preventing and controlling the oryza sativa based on the artemisia apiacea extracts, in-vitro experiments verify that the artemisia apiacea extracts with different concentrations (10-1000mg / L) can remarkably promote apoptosis of oryza sativa somatic cells, destroy microstructures of the oryza sativa somatic cells and inhibit physiological activity, and a new way is provided for developing novel green anti-parasitic drugs.

The invention relates to the technical field of oral care, in particular to a night spray type anti-caries spray as well as a preparation method and application of the night spray type anti-caries spray. 0.1%-0.3% of a grapefruit seed extract; 0.1 to 0.5 percent of sodium hyaluronate; 2-5% of xylitol; 0.02 to 0.05 percent of menthol; 0.05 to 0.12 percent of dipotassium glycyrrhizinate; 0.05 to 0.10 percent of potassium sorbate; through the synergistic effect of the sodium fluoride, the grapefruit seed extract and the xylitol, a three-dimensional protection system capable of promoting remineralization, resisting bacteria in a broad spectrum and specifically inhibiting deformation hammer balls is formed, and the anti-caries effect at night is remarkably improved.

The present invention relates to a method for treating Gram-negative bacteria that are resistant to colistin (MIC > 50 mg / mL), an antibacterial compound, excluding those of the genera Burkholderia, Proteus, and Serratia, comprising contacting the bacteria with an aqueous pharmaceutical composition containing a rose bengal (RB) compound of formula I, dissolved or dispersed at a concentration of about 0.01 to about 15 mg / mL, and irradiating the contacted bacteria with light of a wavelength of about 500 nm to about 600 nm for a period of about 1 to about 10 minutes, at a concentration of about 16 to about 160 J / cm². 2 The aim is to create a method that involves obtaining a certain amount of light to treat and kill irradiated bacteria. [Formula 1] JPEG2026511692000028.jpg5864 (where X, R 1 , R 2 (and M+ are defined herein)

The invention relates to a bacterial strain composition for treating toilet sewage and application of the bacterial strain composition, the bacterial strain composition for treating toilet sewage comprises a Pseudomonas tawanensis EN-F2 bacterial strain, an Acinetobacter jonsonii EN-J1 bacterial strain, a Glutinobacter arisenensis EM-H8 bacterial strain, a Leucobacter denitrificans FST-3 bacterial strain, a Providencia husteriensis FST-4 bacterial strain, a Comamonas testosterone FST-9 bacterial strain and a Proteus cibi FST-12 bacterial strain, the preservation number of the Pseudomonas tawanensis EN-F2 bacterial strain is CCTCC NO: M 20211515, and the preservation number of Acinet is CCTCC NO: M 20211515. The preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20241977, the preservation number of the strain is CCTCC NO: M 20241978, the preservation number of the strain is CCTCC NO: M 20241978, the preservation number of the strain is CCTCC NO: M 20241975, the preservation number of the strain is CCTCC NO: M 20241976, the preservation number of the strain is CCTCC NO: M 20241976, the preservation number of the strain is CCTCC NO: M 20241977, the preservation number of the strain is CCTCC NO: M 20241977, the preservation number of the strain is CCTCC NO: M 20241977, and the preservation number of the strain is CCTCC NO: M 20241977. The strain composition disclosed by the invention has the capability of efficiently degrading NH4 < + >-N, NO3 <->-N and COD (Chemical Oxygen Demand).

The present invention provides a lung squamous cell carcinoma patient immunotherapy effect prediction device, which comprises a detection unit and a data analysis unit, the detection unit extracts 9 types of intra-tumor bacteria as characteristic intra-tumor bacteria based on 16S rRNA sequencing data of a lung squamous cell carcinoma patient tumor puncture sample; the data analysis unit is used for constructing a data model for predicting the curative effect of immunotherapy on the basis of 16S rRNA sequencing data of tumor puncture samples of the characteristic intra-tumor bacteria and lung squamous cell carcinoma patients; the characteristic intra-tumorous bacteria comprise a plasmobacterium actinomycetes genus, an anaerobic bacillus genus, a kantobacterium genus, a Vinci bacterium genus, a kocuria genus, a freshwater bacterium genus, a microbacterium genus, an achromobacter genus and a proteus genus; the prediction device can predict whether immunotherapy of lung squamous cell carcinoma patients is effective or not, screening and hierarchical management can be conducted on the patients before treatment, and therefore individualized medication is guided, and the curative effect is improved.

This invention discloses primers, kits, methods, and applications for detecting pathogenic *Pseudomonas proteus* strains based on the *oprL* gene, belonging to the field of microbial detection technology. This invention provides specific primers for detecting pathogenic *P. proteus* strains based on the *oprL* gene. The primers are designed based on the differential patterns identified after multiple sequence alignment, specifically by analyzing the results of multiple sequence alignments of all *P. proteus* *oprL* gene sequences in the NCBI database. The nucleotide sequences of the primers are shown in SEQ ID No. 1, SEQ ID No. 2, and SEQ ID No. 3. The *oprL* gene of *P. proteus* is amplified by PCR, and the PCR amplification products are then subjected to electrophoresis. The pathogenicity of *P. proteus* can be effectively distinguished based on the electrophoresis image, enabling rapid detection of *P. proteus* infecting large yellow croaker. The primers have good specificity, and the detection method is simple and intuitive.

The invention discloses an artificial microbial multicellular high-ammonia nitrogen wastewater treatment bacterial community, a promoter, a construction method and an application thereof. The bacterial community comprises at least two of Bacillus haynesii strain ZJB21169, Bacillus subtilis strain ZJB21170, Stenotrophomonas rhizophila strain ZJB21172, Proteus alimentorum strain ZJB21171 and Paracoccus kondratievae strain ZJB21134. The composite bacterial community is added to a prepared nutrient agent to obtain a microbial nutrient agent, which is used for denitrification in a sewage treatment system. The nutrient enhancer comprises, by weight, 245-450 parts of amino acids, 90-223 parts of metal salts, 0-50 parts of choline and 0-50 parts of B vitamins. The present invention adds a self-developed promoter to assist the GZBS process in strengthening the denitrification process of micro-aerobic activated sludge, and establishes an artificial microbial multicellular biochemical treatment system with synchronous nitrification and denitrification bacteria as the community advantage. The system has high denitrification efficiency and no secondary pollution. It has broad application prospects in biological denitrification treatment, especially for the denitrification treatment of high-ammonia nitrogen landfill leachate.

The application discloses acetic acid-producing proteobacteria and application thereof in preparation of feed additives. 35 strains with good fiber degradation function are obtained from Holstein cow rumen fluid through preliminary screening, and bran medium is used for screening the 35 strains, and it is found that 25 strains have the ability of converting complex carbon source into reducing sugar, and the most powerful strains of different genera are selected for in-vitro fermentation verification, and the fiber utilization efficiency of the strains on ruminant TMR is observed, and it is found that the acetic acid-producing proteobacteria (microbial preservation number is CGMCC No. 33516) has the most powerful function on feed dry matter degradation rate and fiber degradation rate, and can improve rumen fermentation liquid pH value, and significantly improve the content of acetic acid, isobutyric acid, butyric acid, valeric acid and total rumen volatile fatty acid. The acetic acid-producing proteobacteria provided by the application has application prospect in improving feed fiber utilization rate or improving animal digestion capacity.

The invention discloses an L-amino acid deaminase mutant and application thereof. The invention verifies that the L-amino acid deaminase derived from proteus haunhai has the capability of catalyzing L-glutamic acid to generate alpha-KG for the first time. Through protein three-dimensional structure simulation and the like, it is predicted that related sites possibly influencing catalysis and combination are I64, G257, Q278 and M440, site-saturated mutation is carried out, and a mutant and engineering bacteria for efficient catalytic synthesis of alpha-KG are obtained. Compared with a wild type, the catalytic activity of the mutant for catalyzing L-glutamic acid to generate alpha-ketoglutaric acid is remarkably improved, and the mutant has a wide prospect of large-scale industrial application.

The invention belongs to the technical field of biology, and discloses proteus mirabilis, application of metabolite of the proteus mirabilis and anti-tumor medicine.Tests find that thalli and the metabolite of the proteus mirabilis have a good inhibiting effect on part of tumors, and it is found in the in-vivo test process that the metabolite of the proteus mirabilis has a good inhibiting effect on part of tumors. The metabolite of the proteus mirabilis has a very prominent inhibition effect on melanoma.

The application relates to the technical field of rapid detection methods, in particular to a rapid detection method of proteus mirabilis which is not for disease diagnosis or treatment purposes, first, the RPA reaction system and the CRISPR / Cas12a reaction system are respectively placed at the bottom of a PCR reaction tube and the inner side of the tube cover, so that the competition between CRISPR / Cas12a and RPA is avoided, the reaction rate and sensitivity are ensured, and because the tube cover does not need to be opened, the risk of nucleic acid aerosol pollution is also avoided; second, the RPA provided by the application specifically amplifies target DNA through preferred primers, the CRISPR / Cas12a specifically recognizes a target sequence through preferred crRNA, and under preferred reaction conditions, through the double screening of the RPA and the CRISPR / Cas12a, the proteus mirabilis can be rapidly, sensitively and specifically detected, the reliability is high, the cross reactivity is low, and the application is convenient to popularize.

The application discloses a plant salt-tolerance promoting active metabolite separated from a rhizosphere growth-promoting Pseudomonas proteus and application thereof. The metabolite is dibutyl phthalate (DBP) with a molecular formula of C 16 H 22 O4, has the effect of improving the salt tolerance of rice, and obviously increases the plant height, root length, aboveground fresh weight and underground fresh weight of the rice, is stable in effect, and has a good application prospect; and can be used as a preparation for improving the salt tolerance of crops in agricultural green production.

The invention discloses L-amino acid deaminase as well as a mutant and application thereof. The invention verifies that the L-amino acid deaminase derived from proteus haunhai has the capability of catalyzing L-glutamic acid to generate alpha-KG for the first time. Through protein three-dimensional structure simulation and the like, it is predicted that related sites possibly influencing catalysis and combination are I64, G257, Q278 and M440, site-saturated mutation is carried out, and a mutant and engineering bacteria for efficient catalytic synthesis of alpha-KG are obtained. Compared with a wild type, the yield of alpha-KG generated through catalysis of the mutant LAAD-phRK is increased by 4.53 times. 1.5 g / L of LAAD-phRK whole cells react in a reaction system containing 10 mM of L-glutamic acid and 10 mM of calcium ions at the temperature of 30 DEG C and the pH value of 8.0 for 8 h, L-glutamic acid with the concentration of 6 mM or above can be converted, the molar yield of alpha-KG is 60% or above, and the LAAD-phRK whole cells have wide prospects in large-scale industrial application.

The invention discloses a bordetella bacteriophage with good acid tolerance as well as a composition, a kit and application of the bordetella bacteriophage, and belongs to the technical field of bacteriophages. The bacteriophage is bordetella bacteriophage BOP2ABONT11, the preservation number of the bacteriophage BOP2ABONT11 is GDMCC NO.67044-B1, and the bacteriophage BOP2ABONT11 has a nucleotide sequence as shown in SEQ ID No.1. The bacteriophage has a polyhedral stereosymmetric head and a short contracted tail, is strong in lysis capability, can identify 92% of bordetella, and has titer of 4.2 * 10 PFU / mL after being cultured for 8 hours under the condition that MOI is 0.001; the acid resistance and heat resistance are excellent, and the catalyst still has activity after being treated for 96 hours at pH 3 and is stable at 55-65 DEG C; the strain does not contain virulence genes, is high in safety and has no splitting action on non-host bacteria. The invention also provides a composition containing the bacteriophage, and a biological bactericide, a medicine, a feed additive and a kit prepared from the bacteriophage or the composition. The bacteriophage and related products can efficiently prevent and treat Bordetella infection, are applied to the fields of environment disinfection, food preservation, animal breeding, pathogenic bacterium detection and the like, and have wide application prospects.

This invention relates to a strain of *Pseudomonas proteus* PHBA133 and its applications, belonging to the field of bioengineering. The strain, classified as *Pseudomonas proteus* coglossicida PHBA133, was deposited at the China General Microbiological Culture Collection Center on December 26, 2022, with accession number CGMCC No. 26275. The *Pseudomonas proteus* PHBA133 provided by this invention exhibits strong genetic stability and a broad substrate spectrum. It can ferment and convert p-hydroxycinnamic acid and its derivatives into corresponding aldehydes, with a conversion concentration of p-hydroxybenzaldehyde as high as 3.4 g / L, demonstrating potential for industrial application. The production process is simple, mild, green, and safe, avoiding the problems of long reaction times, high energy consumption, and environmental pollution faced by traditional chemical methods. Furthermore, it produces fewer byproducts, facilitating extraction and separation operations.

This invention provides a *Pseudomonas plecoglossicida* strain, its inoculant, and its applications, relating to the field of microbiology. Specifically, the *Pseudomonas plecoglossicida* NT strain, with accession number CGMCC No. 25090, can efficiently convert nitrate nitrogen, reduce the accumulation of nitrates in soil, and remediate secondary salinized soils. It also possesses the ability to produce extracellular polysaccharides, promoting the formation of soil aggregates and increasing soil cohesion. Furthermore, this strain can successfully colonize the rhizosphere soil of vegetables, improving the rhizosphere ecological environment, promoting vegetable growth, and increasing yield. This achieves the goal of ensuring both the sustainable development of modern agriculture and the quality and quantity of crops. The strain of this invention has relatively comprehensive soil remediation capabilities and has enormous application potential.

The invention relates to the technical field of rapid detection methods, in particular to a rapid detection method for proteus mirabilis with a non-disease diagnosis or non-treatment purpose, firstly, an RPA reaction system and a CRISPR / Cas12a reaction system are respectively placed at the bottom of a PCR reaction tube and on the inner side of a tube cover, so that the competitive effect of CRISPR / Cas12a and RPA is avoided, and the detection accuracy is improved; the reaction rate and the sensitivity are ensured, and meanwhile, the risk of nucleic acid aerosol pollution is also avoided because the cover does not need to be opened; secondly, the RPA provided by the invention specifically amplifies a target DNA through a preferred primer, the CRISPR / Cas12a specifically recognizes a target sequence through preferred crRNA, under preferred reaction conditions, through double screening of the RPA and the CRISPR / Cas12a, rapid, sensitive and specific detection of proteus mirabilis can be realized, the reliability is high, the cross reactivity is low, and the method is convenient to popularize.

The application provides a general Proteus, a microbial composition for water body sediment treatment and application thereof, and relates to the technical field of sewage treatment. Proteus vulgaris ​ The NB37 can secrete exocellular polysaccharide to produce flocculation effect, effectively settling suspended matters; meanwhile, the NB37 can secrete cellulase, and can actively degrade agricultural solid wastes such as corncobs, so that carbon sources can be self-sufficiently released; in addition, the strain can promote the growth of aquatic plants, and forms positive feedback cooperation with an ecological floating bed; technical effects of improving water body transparency, enhancing the self-purification capacity of the water body and realizing long-acting treatment are achieved.

The present invention contemplates a method for treating gram-negative bacteria other than the genus Burkholderia, Proteus and Serratia, said gram-negative bacteria also exhibiting drug resistance (MIC > = 50 mg / mL) against the bacterial compound colistin, said gram-negative bacteria also exhibiting drug resistance (MIC > = 50 mg / mL) against the bacterial compound colistin, such as colistin, colistin, colistin, colistin, colistin, colistin, colistin, colistin, colistin, colistin, colistin, colistin and colistin. The method comprises contacting bacteria with an aqueous pharmaceutical composition containing a rose-bengal (RB) compound of the following formula I dissolved or dispersed therein at a concentration of from about 0.01 to about 15 mg / mL, and irradiating those contacted bacteria with light having a wavelength of from about 500 nm to about 600 nm for a period of from about 1 to about 10 minutes to provide a light dose of from about 16 to about 160 J / cm2, treating and killing the irradiated bacteria, wherein X, R1, R2 and M + are defined herein.

The invention relates to the technical field of rapid detection methods, in particular to a one-step detection method for proteus mirabilis with a non-disease diagnosis or non-treatment purpose, recombinase polymerase amplification provided by the invention specifically amplifies target DNA through primers, CRISPR / Cas12a specifically recognizes a target sequence through crRNA, and through double screening of RPA and CRISPR / Cas12a, the proteus mirabilis can be rapidly detected. And rapid, sensitive and specific detection of proteus mirabilis can be realized. Meanwhile, an RPA primer, crRNA and reaction conditions are optimized, so that an RPA reaction system and a CRISPR / Cas12a reaction system can be premixed in a tube, and the reaction can be completed only by a one-step method. The method can specifically detect proteus mirabilis and has no cross reaction with common pathogenic bacteria, optimized conditions can avoid the competitive effect of CRISPR / Cas12a and RPA, the reaction rate and sensitivity are guaranteed, and meanwhile, the risk of nucleic acid aerosol pollution is also avoided due to the fact that uncovering is not needed.

The invention belongs to the technical field of bacteriophages, and discloses an aquatic product source common proteus bacteriophage PF107, a composition thereof and application of the aquatic product source common proteus bacteriophage PF107, the preservation number of the aquatic product source common proteus bacteriophage PF107 is CGMCC No.46161, and the aquatic product source common proteus bacteriophage PF107 is preserved in the China General Microbiological Culture Collection Center on August 16, 2024, and the preservation number is CGMCC No.46161. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacteriophage PF107 has excellent splitting performance on common proteusbacillus vulgaris of various different aquatic products, is wide in splitting spectrum and high in titer, can be prepared into drugs, environment disinfectants, feed additives or aquatic product preservatives for preventing and treating the proteusbacillus vulgaris, can effectively prevent and treat propagation and secondary infection of the proteusbacillus vulgaris, and can be used for preparing medicines, environment disinfectants, feed additives or aquatic product preservatives for preventing and treating the proteusbacillus vulgaris. The death and culling rate of aquatic products can be effectively reduced, the positive rate of proteusbacillus vulgaris antibodies is reduced, the method is green and environment-friendly, and the problems of antibiotic residues and pathogenic bacterium drug resistance caused by use of antibiotics are avoided.

The invention relates to the technical field of preparation of p-hydroxyphenylacetic acid, in particular to a method for preparing p-hydroxyphenylacetic acid. On the first aspect, a proteus hauderi strain OMK-101 is provided, the proteus hauderi strain OMK-101 can convert L-tyrosine into p-hydroxyphenylacetaldehyde and a small amount of p-hydroxyphenylacetic acid, and the preservation number of the proteus hauderi strain OMK-101 is CCTCC (China Center for Type Culture Collection) NO: M 20252832. On the second aspect, the invention provides a bacillus tropicus strain OMK-102 which can be used for converting p-hydroxyphenylacetaldehyde into p-hydroxyphenylacetic acid, and the preservation number of the bacillus tropicus strain OMK-102 is CCTCC (China Center for Type Culture Collection) NO: M 20252833. In the third aspect, the invention provides an application of the OMK-101 in the first aspect or the OMK-102 in the second aspect in preparation of p-hydroxyphenylacetic acid. In the fourth aspect, the invention provides a method for preparing p-hydroxyphenylacetic acid. By establishing a mixed strain whole-cell catalysis system containing OMK-101 and OMK-102, L-tyrosine can be efficiently converted into p-hydroxyphenylacetic acid, the yield and the yield are high, and the L-tyrosine selected as a substrate is cheap and easy to obtain.

According to the multiple enrichment enrichment and multiple fluorescent PCR detection method for the hospital-infected gram-negative bacteria, the gram-negative bacteria composite enrichment liquid provided by the invention can provide better nutritional conditions for common hospital-infected gram-negative bacteria and inhibit the growth of gram-positive bacteria, and a freeze-drying type multiple fluorescent PCR detection reagent is combined, so that the detection efficiency is improved, and the detection cost is reduced. One-time sampling, one-time enrichment and one-time fluorescent PCR detection on four gram-negative bacteria, namely klebsiella pneumoniae, pseudomonas aeruginosa, proteusbacillus vulgaris and acinetobacter baumannii, in a hospital environment can be realized.

The application provides a device for predicting the curative effect of immunotherapy for lung squamous carcinoma patients, which comprises a detection unit and a data analysis unit, wherein the detection unit extracts 9 intratumor bacterial genera as characteristic intratumor bacterial genera based on 16S rRNA sequencing data of a tumor puncture sample of a lung squamous carcinoma patient; the data analysis unit constructs a data model for predicting the curative effect of immunotherapy based on the characteristic intratumor bacterial genera and the 16S rRNA sequencing data of the tumor puncture sample of the lung squamous carcinoma patient; and the characteristic intratumor bacterial genera comprise Actinoplanes, Anaerobisporus, Cato, Fingoldia, Koch, Limnohabitans, Pararhodobacter, Achromobacter and Proteus. The prediction device can predict whether the immunotherapy for lung squamous carcinoma patients is effective, and can be used for screening and stratified management of patients before treatment, so as to guide individualized medication and improve the curative effect.

The present application relates to the technical field of food processing, and particularly relates to a sodium hyaluronate drink and a preparation method thereof. The drink comprises a plant extract and sodium hyaluronate; the mass ratio of the plant extract to the sodium hyaluronate is (3.2-3.8):3; the plant extract and the sodium hyaluronate are dissolved in water to prepare a 0.08-0.12 mg / mL drink, namely the sodium hyaluronate drink. The two natural sugar substitutes in the technical scheme of the present application do not participate in the carbon metabolic process of the human body, do not produce glycosylation with hemoglobin, and the pancreas secretes a large amount of insulin under the stimulation of pheromones, which helps to regulate the internal balance of the human body. Under the joint regulation of the duodenum and the gallbladder, the probiotics become active, the pathogenic bacteria such as proteus and pseudomonas are inhibited by butyrate produced in the metabolic process of probiotics, and the number is relatively reduced, the inflammation in the intestinal tract is reduced, the flora is more stable, and the immunity can be improved.

The invention belongs to the technical field of bacteriophages, and discloses a proteus mirabilis bacteriophage PF09 and a bacteriophage composition and application thereof, the preservation number of the proteus mirabilis bacteriophage PF09 is CGMCC NO.45897, the proteus mirabilis bacteriophage PF09 is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 22, 2024, the proteus mirabilis bacteriophage PF09 has a wide lysis spectrum, and the proteus mirabilis bacteriophage PF09 can be used for preparing a proteus The compound has a splitting property on proteusbacillus vulgaris of different sources, particularly has a specific splitting advantage on pet proteusbacillus vulgaris, can be used as an active ingredient for preparing medicines, feed additives and disinfectants for preventing and treating pet diseases caused by proteusbacillus vulgaris infection, provides a more effective prevention and control scheme for the problem of prevention and control of pet proteusbacillus vulgaris infection, and can be used for preventing and controlling the pet proteusbacillus vulgaris infection. And the problems of antibiotic residues and drug resistance of pathogenic bacteria caused by the use of antibiotics are also avoided.

This invention discloses a strain of *Pseudomonas proteus* and its applications, belonging to the field of microbial technology. The *Pseudomonas proteus* strain is named *Pseudomonas proteus* (…). Pseudomonas plecoglossicida Sg308, deposited on March 1, 2026, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 37822, significantly enhances the insecticidal activity of Bacillus thuringiensis against the Asian corn borer. Its mechanism of action primarily involves entry into the hemocoel through perforations caused by Bacillus thuringiensis, leading to septicemia and thus enhancing the lethal effect of Bt toxin. The combined use of this strain and Bt can significantly improve control efficacy and reduce pesticide usage, showing broad application prospects in the green control of the Asian corn borer.