82 results about "Proteus" patented technology

The invention discloses an isoflavone compound in a tobacco rhizome and a preparation method and application thereof. The preparation method comprises the following steps of: crushing a tobacco rhizome sample, performing ultrasonic extraction for 3 to 5 times by using 95 percent ethanol, combining the extracts, filtering, performing reduced pressure concentration to obtain an extract, primarily separating the extract by using silica gel column chromatography, further separating the extract by adopting high performance liquid chromatography, and thus obtaining the required new compound. Antibacterial activity screening test results show that the compound has strong antibacterial effect on common proteus species, escherichia coli, staphylococcus, bacillus subtilis and the like. The compound used for cigarette tipping paper plays an obvious role in inhibiting microbes for polluting the cigarette tipping paper.

The present invention provides a gene chip for quickly detecting pathogens from food source, discloses the preparation method of said gene chip and provides 26 oligonucleotide probe sequences for detection. Said gene chip can quickly, accurately and high-effectively detect and identify the class of the pathogens in food, and its detection range includes staphylococcus aureus, Shiga's bacillus, salmonella, colibacillus 0157, bacillus proteus, mononuclear hyperplastic listerella, enterocolitis yersinia, aeruginous pseudomonads, vibrio parahaemolyticus, vibrio cholerae, bacillus cereus, beta hemolytic streptococcus, coconut fermentation pseudomonads, boticin, vibrio jejuni and bacillus perfringens, etc.

The invention discloses application of a Harmine derivative to preparation of antibacterial medicines. The bacteria is selected from Acinetobacter, Bacillus, Campylobacter, Chlamydia, Chlamydia trachomatis, Clostridium, Citrobacter, Escherichia, enterohemorrhagic escherichia coli, enteric bacteria, Enterococcus, Francisella, Haemophilus, helicobacter, Klebsiella Bacillus, Lester monocytogenes, Moraxella, Mycobacterium, Neisseria, proteus, Pseudomonas, Salmonella, shewanella oneidensis, Shigella, Stenotrophomonas, Staphylococcus, Streptococcus and Yersinia.

The invention relates to nucleotide special to an Internal transcribed spacer (ITS for short) of a 16S rRNA-23S rRNA gene in Proteus mirabilis, in particular to oligonucleotide special to the ITS in the Proteus mirabilis and application thereof. The invention also provides a PCR detection reagent taking an oligonucleotide pair as a primer and a detection method thereof. The detection method utilizes the PCR reagent to detect the Proteus mirabilis in human body and the environment, is simple, convenient and quick, has good specificity and high sensitivity, can be used in the fields of supervision and detection of food and clinical samples, detection of pathogen in the food, microbial classification and epidemiological investigation and so on, and has deep social benefit and large economic benefit.

The invention discloses a specific nucleic acid marking sequence of proteus mirabilis, a PCR detecting method and the application. The aim of the invention is to provide the specific nucleic acid marking sequence of the proteus mirabilis, a qualitative and quantitative PCR detecting method of the proteus mirabilis and the application in preparing qualitative and quantitative PCR detecting kits of the proteus mirabilis. The specific nucleic acid marking sequence of the proteus mirabilis is one of following nucleic acid sequences: firstly, a DNA sequence of SEQ ID NO: 1 in the table, secondly, a restricted DNA sequence of the SEQ ID NO: 1 in the table, which has the homology more than 90% and is the specific nucleic acid sequence of the proteus mirabilis, and thirdly, a nucleic acid sequence which can cross-breed with the restricted DNA sequence of the SEQ ID NO: 1 in the table under the high-stringent condition. The invention can be used in qualitative and quantitative molecular detection of the proteus mirabilis (including the detecting methods of ordinary PCR, quantitative PCR, chips, hybrid and the like) and has broad application prospect.

The invention discloses a method for producing a feed through pretreating at a high temperature and fermenting at a low temperature. The method comprises the following steps: first utilizing amylases, celluloses, hemicellulase, bacillus coagulans, bacillus licheniformis, tropophilous bacilli, proteus hause, chelating cocci, lignocellulolytic microorganisms and the like as degrading factors, and degrading starch, celluloses and hemicelluloses in the materials into monosaccharides or polysaccharides at 40-70 DEG C; then inoculating the materials with candida utilis, monilia tropicalis, saccharomyces cerevisiae, lactobacilli and bacillus subtilis by a fermenting technology, and fermenting at 20-40 DEG C to make a high-protein feed with oligomerization functional sugar for cattle, sheep, pigs, chicken or other animals. The method can solve the insufficient protein problem of animal husbandry, enhance animal constitutions, relieve the pressure that people and livestock contest with crop and reduce pollution, and has significant economical and social benefits.

A state, particularly a disease state, associated with the production of volatiles is detected by passing a sample containing the volatiles to a single sensor. This may be a semiconductor gas sensor element or a surface acoustic wave device. This provides an output signal, e.g. in the form of a tailing peak. A plurality of characteristics of the signal (e.g. peak height and maximum positive gradient) are measured to characterise the sample and hence the underlying state. For example we can discriminate between urine samples which are (a) infected with proteus, (b) infected with E. coli or (c) uninfected.

The invention discloses a bacterial isolates relative to suppression of a biofilm and detoxification. The bacterial isolates relative to suppression of the biofilm and detoxification, which is provided by the invention, is especially Proteus mirabilis (Proteus mirabilis)10#, wherein the preservation number of Proteus mirabilis10# in China General Microbiological Culture Collection Center is CGMCC No.6426. The Proteus mirabilis10# CGMCC No.6426, which is provided by the invention, has effects of inhibiting formation of the pathogenic bacteria biofilm and detoxifying. The invention has the following advantages that: (1) through active ingredients (metabolites) of a Proteus mirabilis10# CGMCC No.6426 preparation, growth of the pathogenic bacteria is not influenced, the force is not generated, and the tolerance probability is low; and (2) through active ingredients (metabolites) of a Proteus mirabilis10# CGMCC No.6426 preparation, formation of the pathogenic bacteria biofilm is inhibited and the toxicity of the pathogenic bacteria is reduced.

The invention provides proteus for reducing Fe(III) in clay mineral and application thereof in inhibition of expansibility of clay. The proteus provided by the invention has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 15331 and the strain has iron reduction activity, facultative anaerobic performance and relatively wide utilization of electron donors; the Fe(III) in ferric citrate and montmorillonite also can be efficiently reduced; the proteus can be used for corroding the clay mineral in low-permeability oil reservoirs, and also can be used for reducing trivalent iron ions in expanded clay mineral, which is easily hydrated in the oil reservoirs, through the strain and a metabolic product thereof; changes of a lattice structure and phase transformation are carried out, so that the aims of improving the permeability, realizing channel drag reduction and improving the crude oil recovery ratio are realized.

The invention discloses a pseudomonas proteus znuA gene stable silencing strain and application. The pseudomonas proteus znuA gene stable silencing strain is constructed through a gene silencing technology, then the pathogenicity of the znuA gene stable silencing strain to epinephelus coioides is determined through an artificial infection experiment, transcriptome data of spleens of the epinephelus coioides infected by a wild strain and the znuA gene stable silencing strain is analyzed by utilizing a dual RNA-seq technology, meanwhile, transcriptome data of hosts and pathogenic bacteria is researched, the function of a znuA gene in interaction of pseudomonas proteus and the epinephelus coioides is discussed from the transcriptome level, and then the influence of the znuA gene on the toxicity of the pseudomonas proteus is disclosed. The constructed strain has the advantages that the pathogenicity to the epinephelus coioides is remarkably reduced, and the transcriptome data of the pseudomonas proteus and the epinephelus coioides in the infected spleens can be remarkably changed, so that the strain can be used for researching the pathogenic mechanism of the pseudomonas proteus.

The invention discloses a method for preparing a natural red jujube skin pigment and application of the natural red jujube skin pigment. The method comprises the following steps of: removing jujube flesh from red jujubes to obtain red jujube skin; drying the red jujube skin, crushing, leaching in a weak base solution, and filtering to obtain a red jujube skin pigment stock solution; and concentrating, drying and crushing the red jujube skin pigment stock solution to obtain powdered natural red jujube skin pigment. According to the method, a process is simple and low in cost and is suitable for large-scale production; and the powdered red jujube skin pigment ensures the stability and wide application of the pigment. The prepared natural red jujube skin pigment has the antibacterial activity on gram-positive bacteria of staphylococcus aureus, listeria monocytogenes, lactobacillus, alicyclobacillus and the like and on gram negative bacteria of escherichia coli, proteus, shigella dysenteriae, pneumobacillus, acinetobacter and the like.

The invention discloses a method for planting rhizoma polygonati. The method comprises following steps: land selection, land preparation, rhizome selection and treatment, rhizome planting, field management and harvesting. In the treatment of rhizomes, potassium hydroxide solution is first used to sterilize the rhizomes, and then self-made treatment liquid is used to soak the rhizomes and further sterilize the rhizomes, so as to improve the antibacterial ability of rhizomes. The cortex moutan in the self-made treatment liquid contains gallic acid, which has a strong inhibitory effect on variousfungi such as Bacillus subtilis and Proteus. The composition contains grapefruit peel, elsholtzia splendens leaf and pearl plum, and the volatile oil of the three also has a strong smoke-killing effect on pests and colonies. The rhizome of Polygonatum sibiricum is sterilized with potassium hydroxide solution and then soaked in self-made treatment liquid to kill harmful bacteria and microorganismscarried by rhizome of Polygonatum sibiricum, which greatly enhanced its antibacterial ability, avoided the rot and mildew of rhizome, and increased the rate of seedling emergence.

The invention relates to a bacilli, its compound preparation and the preparing method for the compound preparation. Its name is Bacillus subtilis LY-35, which is preserved in the general microbe center of Micro Germ conservation Management committee of China, and its conservation number is:CGMCC N0.1222. The preparation is a mixture of lambda phage of culture of bacilli and staphylococcus, streptococcus, colibacillus, bacillus proteus, pseudomonad, salmonella, pasteurella and klebsiella, can cure and prevent all kinds of illness caused by conditional pathogen,is a micro biological preparation which can be used by being mixed with antibiotics or replace antibiotics without any remainder and pollution, with good curative effect and safety.

The invention discloses a method for high-throughput screening of alpha-keto acid high-producing strains, and belongs to the technical field of bioengineering. The method comprises the steps that protein engineering modification is carried out on the L-amino acid deaminase PmiLAAD derived from proteus mirabilis, a high-throughput screening method is used, a recombinant strain and a strain mutant of the L-amino acid deaminase PmiLAAD containing proteus mirabilis are cultured in a 96-well plate, transfer induction is carried out, cells of the L-amino acid deaminase PmiLAAD containing proteus mirabilis are centrifugally collected to convert L-amino acids (L-valine, L-methionine and L-phenylalanine) to generate corresponding alpha-ketonic acid, developing is carried out by utilizing 2, 4-dinitrophenylhydrazine, a light absorption value is read at 520nm, shake flask verification is carried out on a bacterial strain with a large light absorption value, and screening is carried out to obtain the bacterial strain with significantly improved alpha-keto acid yield.

The present invention provides a dampness-eliminating itching-relieving Chinese medicine preparation with the action of inhibiting dermatomyces, trichophyton violaceum, trichophyton concentricum, achorion schoenleini and bacteria of staphylococcus aureus, hemolytic streptococcus, colibacillus, bacillus pyocyaneus and bacillus proteus, etc. for curing dermatosises with obvious therapeutic effect. Said Chinese medicine preparation is made up by using 10 Chinese medicinal materials of kochia fruit, cnidium seed, silkworm, dictamnus root bark, capillaries, schizonepeta spike and others through a certain preparation process.

The inventiona relates to bromo-2, 4í»-dihydroxyl diphenyl ether compound and its synthesizing method, using 2-methoxy phenol and 4-methoxy chlorobenzene as raw materials, and it is an antimicrobial obtained by three-step reaction, and has better antimicrobial activity to Escherichia coli, catarrh diplococcus, Staphylococcus aureus, Staphylococcus Albus, bacillus proteus, etc. and avoids producing highly toxic dioxin compound in the producing and using course, avoiding environmental pollution and damaging human health; and also overcomes the shortages that natural bromic2, 4í»-dihydroxyl diphenyl ether antimicrobial can form highly toxic dioxin compound in use on certain conditions, and the natural bromic2, 4í»-dihydroxyl diphenyl ether antimicrobial has limited source, complex extracting course and high cost..

The invnetion discloses a method for cultivating rosemary. The method provided by the invention comprises the following steps of: 1), irrigating the rosemary from the end of October to the beginning of November in the north area, at the same time, erecting a plastic shelter, and covering coverings on the outer surface of the plastic shelter; 2), removing the coverings treated in the step 1) at the end of February in the next year; and 3), removing the plastic shelter treated in the step 2) at the end of March in the next year and irrigating the rosemary. Experimental results show that the method disclosed by the invention performs cold-proof cultivation in Beijing, and avoids the cultivation difficulty in the north area; the bacteriostatic activity of an essential oil extracted from the cultivated rosemary is high, so that the essential oil has inhibition effects on escherichia coli, staphylococcus aureus, bacillus subtilis and proteus.

The invention discloses recombinant proteus pseudomonadaceae for producing L-xylose and application thereof, and belongs to the technical field of bioengineering. A synthesized 2-ketogluconate reductase gene, a 2,5-diketogluconate reductase gene from corynebaterium ATCC 31090, and a pyruvate decarboxylase gene from saccharomycescerevisiae are successfully expressed in host proteus pseudomonadaceaeby a double-plasmid system; after the obtained gene engineering strain is fermented for 56h on a shaking bottle, the output of L-xylose can reach 16.2g / L, and the conversion rate reaches 20.3%; afterthe obtained gene engineering strain is respectively fermented for 48h and 44h on a 3L fermenting tank and a 15L fermenting tank, the outputs of L-xylose can respectively reach 37.6g / L and 45.8g / L, and the conversion rates of glucose can respectively reach 40.7% and 57.3%. The method has the advantages that the cost of the raw materials is low, the pollution to environment is avoided, the operation is simple, and the important economic benefits and social benefits are realized.

This disclosure provides a method for transporting a pathogen under ambient conditions, by culturing the pathogen with an amoeba under conditions that favor the incorporation of the pathogen into a trophozoite, starving the amoeba until it encysts, then culturing under conditions that favor conversion of the amoeba back to a trophozoite. In one aspect, the conditions that favor incorporation of the pathogen into the cyst of the amoeba comprises contacting the pathogen with the amoeba in an iron rich environment. Virus and / or bacteria are pathogens that can be transported by the disclosed method. Amoeba that are useful in the disclosed methods include, without limitation Acanthamoeba castellanii, Hartmannella vermiformis and Naegleria gruberi. The disclosed methods have utility in: transporting pathogens from military field hospitals and clinics to the laboratory; transporting pathogens from global satellite laboratories to clinical laboratories; long term storage of pathogens; enriching contaminated patient samples for pathogens of interest; biosurveillance and detection efforts.

Belonging to the technical field of pharmaceutical chemistry, the invention discloses a naphthalimide benzimidazole compound, a preparation method and application thereof. The compound has strong in-vitro antimicrobial activity, especially shows very high inhibitory activity to staphylococcus aureus, methicillin-resistant staphylococcus aureus, bacillus subtilis, micrococcus luteus and other gram-positive bacteria, escherichia coli, proteus, pseudomonas aeruginosa, salmonella typhi and other gram-negative bacteria, candida utilis, aspergillus flavus, saccharomyces cerevisiae, candida albicans,candida mycoderma bacteria and other fungi, can be used for preparation of antibacterial and / or antifungal drugs, and is conducive to solving increasingly serious drug resistance, stubborn pathogenicmicroorganisms, newly emerging harmful microorganisms and other clinical treatment problems.

The invention discloses a compound bacterial agent for promoting the growth of the flathorned soldier fly larvae and its application. The composite bacterial agent for promoting the growth of the soldier fly larvae provided by the invention comprises: Cochlear bacterium ( Kocuria marina ) FE01, Lysinibacillus boronitolerans FE04, Proteus mirabilis ( Proteus mirabilis ) FE08 and Bacillus subtilis BSF‑CL is used to promote the growth of soldier flies in the process of converting soldier flies into chicken manure, which is safe and environmentally friendly. By adding the agent to the chicken manure containing soldier flies, the weight gain of soldier flies can be increased by 28.57% compared with the conversion system without adding bacteria. Benefits have great economic value.

The method is suitable for the technical field of bacteria detection, the invention discloses a triple TaqMan Real-Time PCR detection method for simultaneously distinguishing proteus mirabilis(P. mirabilis), proteus vulgaris (P.vulgaris) and proteus penesei (P.pennerei), and a kit. The primers and nucleotide sequences of proteusbacillus vulgaris are predicted through computer software, and three pairs (six) of primers capable of being used for simultaneously detecting three proteusbacillus vulgaris and nucleotide sequences of three probes are disclosed. The method has the characteristics of strong specificity, high sensitivity and accurate and reliable result. According to the method, the proteusbacillus vulgaris can be identified as a specific species while the proteusbacillus vulgaris isdetected, the defect that only proteus mirabilis can be distinguished in existing proteusbacillus vulgaris detection is overcome, the proteusbacillus vulgaris detection process is greatly simplified,the workload of operators is reduced, the detection period is shortened, and a powerful technical support can be provided for detecting the proteusbacillus vulgaris in the industries of medical treatment, medical detection and the like.

The invention relates to a method for preparing composite calcium bilirubinate from ox gall. The method provided by the invention comprises the following steps of 1, adjusting taurine content of natural ox gall to 3 to 7% (g / ml), 2, inoculating a culture medium with bacterium liquid comprising one or more of escherichia coli, enterococcocci and proteus, carrying out enlargement culture until the bacteria are in a growth stationary stage, killing the bacteria, carrying out ultrasonic vibration so that the bacteria were broken, mixing the treated bacterium liquid and ox gall, and stirring at a temperature of 30 to 50 DEG C with heat preservation, 3, adjusting a pH value of the mixed solution of the ox gall and the treated bacterium liquid to 3 to 5 by a diluted acid, stirring at a temperature of 30 to 50 DEG C with heat preservation, adding a clear saturated calcium oxide solution into the mixed solution, stirring fully, filtering, and collecting brown-red precipitates, 4, adding bilirubin, cholic acid, deoxycholic acid, zinc sulfate and magnesium sulfate into the brown-red precipitates, and uniformly mixing by stirring, and 5, carrying out refrigeration and then carrying out vacuumdrying. The method provided by the invention further simplifies production processes and improves a product yield.

The invention discloses a microbial water purifying agent for mandarin fish culture water and a preparation method of the agent. The preparation method comprises the following steps: adding 40-50 parts by weight of magnetic silicon spheres of which the surface is modified with C8 and C18 alkyl chains to 200-250 parts by weight of water; adding 100-150 parts by weight of microbial reagents which include 40-60 parts of synechococcus, 40-50 parts of rhodobacter sphaeroides, 30-40 parts of nitrospina, 30-50 parts of nitrococcus, 20-40 parts of sphaerotilus natans, 30-50 parts of pseudomonas aeruginosa, 3-5 parts of leucothrix, 3-5 parts of thioploca, 50-60 parts of amoeba, 30-50 parts of suctorida, 30-40 parts of dunaliella and 30-50 parts of tetraselmis; next, adding 50-80 parts by weight of beef extract peptone agar culture medium for culturing. The microbial water purifying agent is put into a mandarin fish culture pond to purify water in the culture pond.

The invention relates to the technical field of microorganisms, in particular to a Neiella strain, alginate lyase and application of the alginate lyase. The invention provides an alginate lyase producing strain HB171785. The strain belongs to the genus Neiella of gamma proteus, the preservation number is GDMCC No.61001, and alginate lyase produced by the strain has good temperature stability. Theinvention further provides the alginate lyase and a preparation method thereof. The strain HB171785 and the alginate lyase have application potential in degradation of algin and preparation of alginate oligosaccharide.

The invention discloses a traditional Chinese medicine preparation for damp-heat detoxifcation and consists of honeysuckle, dandelion, field pennycress, radix scutellariae, raw coix seed, alisma orientale, red paeonia, lightyellow sophora root and tail of angelica sinensis. The traditional Chinese medicine preparation is prepared by scientific processing technique; by adopting traditional Chinese medicine theory, the traditional Chinese medicine preparation can treat purulent inflammation caused by the retention of damp-heat internally, exogenous affection of pathogenic damp-heat and toxicity and stagnation on the skin, which is proved by clinical application. The honeysuckle, dandelion, field pennycress has the function of clearing away heat and detoxifcation, radix scutellariae can clear away lung heat, red paeonia and tail of angelica sinensis can cool the blood and activate the blood circulation, and raw coix seed and lightyellow sophora root can clear away heat, strengthen the spleen and remove dampness. The invention is a pure traditional Chinese medicine preparation, and has remarkable inhibitory effect on staphylococcus, colon bacillus, streptococcus, pneumococci, meningococcus, proteus and pseudomonas aeruginosa. Furthermore, the invention has convenient therapy and significant clinical effect and diseases are not likely to recur after curing.

The invention belongs to the technical field of medicinal chemistry, and discloses an emodin azole alcohol compound shown as a formula I. The emodin azole alcohol compound has high in-vitro antimicrobial activity and especially has very high inhibitory activity on staphylococcus aureus, bacillus subtilis, micrococcus luteus, escherichia coli, proteus, pseudomonas aeruginosa, shigella dysenteriae,salmonella typhimurium and other bacteria, and candida utilis, candida albicans, saccharomyces cerevisiae, aspergillus flavus, candida albicans and other fungi. The emodin azole alcohol can be used for preparing antibacterial and / or antifungal drugs, so that more efficient candidate drugs are provided for clinical antimicrobial treatment, and the clinical treatment problems of increasingly seriousdrug resistance, stubborn pathogenic microorganisms, newly appearing harmful microorganisms and the like are solved.

The invention discloses a heparin lyase mutant and a recombinant expression method thereof, belonging to the technical field of bioengineering. The heparin lyase III provided by the invention has higher catalytic efficiency and thermal stability, and the catalytic efficiency of the mutant S264F / Y490K / D321N is 1.68 times higher than that of the original strain, which is more conducive to the preparation of low molecular weight heparin. The invention also performs heterologous recombination expression on the heparin lyase III from the Bacteroides proteus, and the intracellular enzyme activity can reach more than 4000U / L.

This invention describes a bacillus subtilis and the process for preparing its compound pharmaceutics. The bacillus subtilis is conserved at China General Microbiological Culture Collection Center of China Committee of Culture Collection for Microorganisms, the name is: Bacillus subtilis LY-35 and the conservation number is: CGMCC No. 1222. The pharmaceutics is a compound of the bacteriophages of such pathogens as bacillus subtilis, Staphylococcus, Streptocoaus, colibacillus, proteus, pseudomonas, salmonella, shigella, pasteurella and klebsiella. The pharmaceutics can prevent and treat the diseases caused by pathogens, and can be a non-polluted, high therapeutic effectiveness and safe substitute for antibiotics.