10 results about "Proteus" patented technology

The present invention relates to a method for treating Gram-negative bacteria that are resistant to colistin (MIC > 50 mg / mL), an antibacterial compound, excluding those of the genera Burkholderia, Proteus, and Serratia, comprising contacting the bacteria with an aqueous pharmaceutical composition containing a rose bengal (RB) compound of formula I, dissolved or dispersed at a concentration of about 0.01 to about 15 mg / mL, and irradiating the contacted bacteria with light of a wavelength of about 500 nm to about 600 nm for a period of about 1 to about 10 minutes, at a concentration of about 16 to about 160 J / cm². 2 The aim is to create a method that involves obtaining a certain amount of light to treat and kill irradiated bacteria. [Formula 1] JPEG2026511692000028.jpg5864 (where X, R 1 , R 2 (and M+ are defined herein)

This invention discloses primers, kits, methods, and applications for detecting pathogenic *Pseudomonas proteus* strains based on the *oprL* gene, belonging to the field of microbial detection technology. This invention provides specific primers for detecting pathogenic *P. proteus* strains based on the *oprL* gene. The primers are designed based on the differential patterns identified after multiple sequence alignment, specifically by analyzing the results of multiple sequence alignments of all *P. proteus* *oprL* gene sequences in the NCBI database. The nucleotide sequences of the primers are shown in SEQ ID No. 1, SEQ ID No. 2, and SEQ ID No. 3. The *oprL* gene of *P. proteus* is amplified by PCR, and the PCR amplification products are then subjected to electrophoresis. The pathogenicity of *P. proteus* can be effectively distinguished based on the electrophoresis image, enabling rapid detection of *P. proteus* infecting large yellow croaker. The primers have good specificity, and the detection method is simple and intuitive.

The application relates to the technical field of rapid detection methods, in particular to a rapid detection method of proteus mirabilis which is not for disease diagnosis or treatment purposes, first, the RPA reaction system and the CRISPR / Cas12a reaction system are respectively placed at the bottom of a PCR reaction tube and the inner side of the tube cover, so that the competition between CRISPR / Cas12a and RPA is avoided, the reaction rate and sensitivity are ensured, and because the tube cover does not need to be opened, the risk of nucleic acid aerosol pollution is also avoided; second, the RPA provided by the application specifically amplifies target DNA through preferred primers, the CRISPR / Cas12a specifically recognizes a target sequence through preferred crRNA, and under preferred reaction conditions, through the double screening of the RPA and the CRISPR / Cas12a, the proteus mirabilis can be rapidly, sensitively and specifically detected, the reliability is high, the cross reactivity is low, and the application is convenient to popularize.

The application discloses a plant salt-tolerance promoting active metabolite separated from a rhizosphere growth-promoting Pseudomonas proteus and application thereof. The metabolite is dibutyl phthalate (DBP) with a molecular formula of C 16 H 22 O4, has the effect of improving the salt tolerance of rice, and obviously increases the plant height, root length, aboveground fresh weight and underground fresh weight of the rice, is stable in effect, and has a good application prospect; and can be used as a preparation for improving the salt tolerance of crops in agricultural green production.

The invention discloses a bordetella bacteriophage with good acid tolerance as well as a composition, a kit and application of the bordetella bacteriophage, and belongs to the technical field of bacteriophages. The bacteriophage is bordetella bacteriophage BOP2ABONT11, the preservation number of the bacteriophage BOP2ABONT11 is GDMCC NO.67044-B1, and the bacteriophage BOP2ABONT11 has a nucleotide sequence as shown in SEQ ID No.1. The bacteriophage has a polyhedral stereosymmetric head and a short contracted tail, is strong in lysis capability, can identify 92% of bordetella, and has titer of 4.2 * 10 PFU / mL after being cultured for 8 hours under the condition that MOI is 0.001; the acid resistance and heat resistance are excellent, and the catalyst still has activity after being treated for 96 hours at pH 3 and is stable at 55-65 DEG C; the strain does not contain virulence genes, is high in safety and has no splitting action on non-host bacteria. The invention also provides a composition containing the bacteriophage, and a biological bactericide, a medicine, a feed additive and a kit prepared from the bacteriophage or the composition. The bacteriophage and related products can efficiently prevent and treat Bordetella infection, are applied to the fields of environment disinfection, food preservation, animal breeding, pathogenic bacterium detection and the like, and have wide application prospects.

This invention relates to a strain of *Pseudomonas proteus* PHBA133 and its applications, belonging to the field of bioengineering. The strain, classified as *Pseudomonas proteus* coglossicida PHBA133, was deposited at the China General Microbiological Culture Collection Center on December 26, 2022, with accession number CGMCC No. 26275. The *Pseudomonas proteus* PHBA133 provided by this invention exhibits strong genetic stability and a broad substrate spectrum. It can ferment and convert p-hydroxycinnamic acid and its derivatives into corresponding aldehydes, with a conversion concentration of p-hydroxybenzaldehyde as high as 3.4 g / L, demonstrating potential for industrial application. The production process is simple, mild, green, and safe, avoiding the problems of long reaction times, high energy consumption, and environmental pollution faced by traditional chemical methods. Furthermore, it produces fewer byproducts, facilitating extraction and separation operations.

This invention provides a *Pseudomonas plecoglossicida* strain, its inoculant, and its applications, relating to the field of microbiology. Specifically, the *Pseudomonas plecoglossicida* NT strain, with accession number CGMCC No. 25090, can efficiently convert nitrate nitrogen, reduce the accumulation of nitrates in soil, and remediate secondary salinized soils. It also possesses the ability to produce extracellular polysaccharides, promoting the formation of soil aggregates and increasing soil cohesion. Furthermore, this strain can successfully colonize the rhizosphere soil of vegetables, improving the rhizosphere ecological environment, promoting vegetable growth, and increasing yield. This achieves the goal of ensuring both the sustainable development of modern agriculture and the quality and quantity of crops. The strain of this invention has relatively comprehensive soil remediation capabilities and has enormous application potential.

The invention relates to the technical field of preparation of p-hydroxyphenylacetic acid, in particular to a method for preparing p-hydroxyphenylacetic acid. On the first aspect, a proteus hauderi strain OMK-101 is provided, the proteus hauderi strain OMK-101 can convert L-tyrosine into p-hydroxyphenylacetaldehyde and a small amount of p-hydroxyphenylacetic acid, and the preservation number of the proteus hauderi strain OMK-101 is CCTCC (China Center for Type Culture Collection) NO: M 20252832. On the second aspect, the invention provides a bacillus tropicus strain OMK-102 which can be used for converting p-hydroxyphenylacetaldehyde into p-hydroxyphenylacetic acid, and the preservation number of the bacillus tropicus strain OMK-102 is CCTCC (China Center for Type Culture Collection) NO: M 20252833. In the third aspect, the invention provides an application of the OMK-101 in the first aspect or the OMK-102 in the second aspect in preparation of p-hydroxyphenylacetic acid. In the fourth aspect, the invention provides a method for preparing p-hydroxyphenylacetic acid. By establishing a mixed strain whole-cell catalysis system containing OMK-101 and OMK-102, L-tyrosine can be efficiently converted into p-hydroxyphenylacetic acid, the yield and the yield are high, and the L-tyrosine selected as a substrate is cheap and easy to obtain.

The application provides a device for predicting the curative effect of immunotherapy for lung squamous carcinoma patients, which comprises a detection unit and a data analysis unit, wherein the detection unit extracts 9 intratumor bacterial genera as characteristic intratumor bacterial genera based on 16S rRNA sequencing data of a tumor puncture sample of a lung squamous carcinoma patient; the data analysis unit constructs a data model for predicting the curative effect of immunotherapy based on the characteristic intratumor bacterial genera and the 16S rRNA sequencing data of the tumor puncture sample of the lung squamous carcinoma patient; and the characteristic intratumor bacterial genera comprise Actinoplanes, Anaerobisporus, Cato, Fingoldia, Koch, Limnohabitans, Pararhodobacter, Achromobacter and Proteus. The prediction device can predict whether the immunotherapy for lung squamous carcinoma patients is effective, and can be used for screening and stratified management of patients before treatment, so as to guide individualized medication and improve the curative effect.

This invention discloses a strain of *Pseudomonas proteus* and its applications, belonging to the field of microbial technology. The *Pseudomonas proteus* strain is named *Pseudomonas proteus* (…). Pseudomonas plecoglossicida Sg308, deposited on March 1, 2026, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 37822, significantly enhances the insecticidal activity of Bacillus thuringiensis against the Asian corn borer. Its mechanism of action primarily involves entry into the hemocoel through perforations caused by Bacillus thuringiensis, leading to septicemia and thus enhancing the lethal effect of Bt toxin. The combined use of this strain and Bt can significantly improve control efficacy and reduce pesticide usage, showing broad application prospects in the green control of the Asian corn borer.