239 results about "Ketoglutaric Acid" patented technology

The invention relates to the technical field of enzyme engineering, in particular to an L-glutamate oxidase mutant as well as preparation and application thereof. Through mutation of the 77th amino acid and the 171st amino acid, the active center configuration of the enzyme is optimized, the binding capacity with a substrate and the catalytic conversion efficiency are enhanced, and the enzyme activity is remarkably improved. The mutant disclosed by the invention shows stronger specificity to L-glutamic acid (salt), effectively reduces non-specific reaction and improves the product purity. And in the presence of a high-concentration substrate, the enzyme activity is not inhibited by the substrate. The prepared L-glutamate oxidase mutant with high enzymatic activity can be efficiently expressed in escherichia coli, the problem that the expression quantity of natural enzymes in streptomyces is limited is solved, the acquisition efficiency of the enzymes is greatly improved, and the production cost is reduced. The mutants can be directly applied to biological manufacturing of alpha-ketoglutaric acid, so that the production efficiency of products is remarkably improved.

The invention relates to a metabolic marker for diagnosing diabetic secondary osteoporosis and application of the metabolic marker, and belongs to the technical field of biological medicine. The method comprises the following steps: firstly, screening out obviously different metabolites between osteoporosis (DOP) and osteoporosis-free groups (DM) in diabetic patients; and secondly, screening out significant difference metabolites between the primary osteoporosis patient and the healthy control group, and excluding the intersection of the two groups of difference metabolites to obtain metabolites which exclude the influence of the primary osteoporosis. Metabolic markers, namely cytosine nucleotide, ketoglutaric acid and triethylamine, are obtained through further screening, and when the three metabolic markers are independently used, AUCgt; the AUC during combined use is 0.99, so that the limitation that the sensitivity of the traditional bone mineral density detection on the osteoporosis recognition in the diabetic population is insufficient is effectively made up.

The invention provides a genetically engineered bacterium for producing O-succinyl-L-homoserine as well as a construction method and application of the genetically engineered bacterium. In a chassis bacterium genome, the expression of a 2-ketoglutaric acid decarboxylase encoding gene sucA is enhanced, and the expression of a succinyl-coenzyme A synthetase encoding gene sucD is weakened, so that the supply of succinyl-coenzyme A is increased; the method comprises the following steps: increasing the NADPH (Nicotinamide Adenine Dinucleotide Phosphate) reducing capacity and ATP (Adenosine Triphosphate) energy supply of a chassis bacterium, increasing DNA (Deoxyribose Nucleic Acid) in combination with a transcription dual regulatory factor ompR to improve the stress resistance of escherichia coli under high osmotic pressure, and introducing an overexpression plasmid containing a homoserine transsuccinylase coding gene metA to construct the genetically engineered bacterium for producing O-succinyl-L-homoserine. The engineering strain obtained through a systematic metabolic engineering modification strategy can realize effective accumulation of OSH, the shake flask yield of OSH reaches 19.8 g / L, the fed-batch fermentation yield of a 5L fermentation tank reaches 110.5 g / L, the sugar-acid conversion rate reaches 52.6%, and a foundation is laid for subsequent construction of high-yield OSH engineering bacteria.

The application relates to an engineering bacterium for high yield of observation blue and a construction method and application thereof, and belongs to the technical field of biological synthesis of natural dyes. The engineering bacterium for high yield of observation blue expresses icd, bpsA, glnA Y405F and gdhA; the engineering bacterium knocks out acnR, yggB, glsK, aceA and ldh. By knocking out the yggB and aceA genes, performing site-directed mutation (Y405F) on the glnA gene, replacing the glsK gene with the glnA Y405F gene, replacing the acnR gene with the icd gene, replacing the ldh gene with the gdhA gene, and integrating the bpsA gene, the application can block the formation of by-products such as lactate and succinic acid in the observation blue synthesis process, improve the flow direction of the citric acid->isocitric acid->alpha-ketoglutaric acid->glutamic acid->glutamine path, and then improve the intracellular glutamine concentration, so that the yield of observation blue is improved.

The invention discloses an endogenous anti-aging composition based on an internal and external synergistic mechanism and a preparation method of the endogenous anti-aging composition. The endogenous anti-aging composition based on the internal and external synergistic mechanism is prepared from the following components in parts by mass: 100 to 200 parts of alpha-ketoglutarate calcium; 100 to 200 parts of an olive leaf extract; 10 to 20 parts of nicotinic acid; 10 to 20 parts of pyrroloquinoline quinone; 20 to 60 parts of pomegranate extract; and 10 to 30 parts of coenzyme Q10. The composition and the preparation method thereof have the beneficial effects that the composition capable of integrally improving the aging state of women from multiple dimensions and the preparation method of the composition are provided.

The invention provides a plasmid, an alpha-ketoglutaric acid engineering strain and a construction method and application of the alpha-ketoglutaric acid engineering strain, the strain is obtained by adopting a directional transformation method, transcriptional levels of katE, katG, sodB and grxA genes are up-regulated, a catR gene and a katA gene are heterologously expressed, a high-copy plasmid pETKG is carried, and the plasmid simultaneously expresses L-glutamate oxidase LGOX genes from Streptomyces sp and Chain A; the strain self-synthesized catalase eliminates H2O2 generated in the process of producing alpha-ketoglutaric acid through enzyme catalysis, engineering bacteria of L-glutamate oxidase LGOX expressed by pETKG plasmids are used for performing whole-cell catalysis on L-sodium glutamate to synthesize alpha-ketoglutaric acid, and the engineering strain is a high-level engineering strain capable of industrially and stably producing alpha-ketoglutaric acid.

A nutraceutical composition, consisting of Nicotinamide Mononucleotide in a range 250-1000 mg, Spermidine in a range 0.5-10 mg, Rhodiola Rosea in a range 200-600 mg, Cacao in a range 1000-10,000 mg, L-Theanine in a range 50-200 mg, Glucosamine in a range 500-3000 mg, and Hyaluronic acid in a range 50-240 mg, or consisting of Epigallocatechin-3-gallate in a range 100-500 mg, Quercetin in a range 250-1000 mg, Resveratrol in a range 150-1000 mg, Sulforaphane in a range 1-50 mg, and Calcium Alphaketoglutarate in a range 300-2000 mg, or consisting of Microdose Lithium in a range 1-5 mg, Trimethylglycine in a range 500-2000 mg, Glutathione in a range 250-1000 mg, Fisetin in a range 100-1000 mg, Urolithin A in a range 250-2000 mg, Sodium Butyrate in a range 100-3000 mg, Berberine in a range 500-1500 mg, Apigenin in a range 50-500 mg, Cinnamon in a range 1000-6000 mg, and Malate in a range 1000-6000 mg.

The invention belongs to the technical field of medicines, and particularly relates to a composition for improving obesity and promoting cardiovascular health as well as a preparation method and application thereof. The composition is prepared from the following components in parts by weight: 5 to 20 parts of glycine betaine, 1 to 5.5 parts of choline chloride, 0.5 to 4 parts of L-theanine, 1 to 15 parts of alpha-ketoglutarate calcium, 0.5 to 3 parts of black ginger extract, 0.05 to 0.25 part of black pepper extract, 0.5 to 3 parts of ginkgo leaf extract and 1 to 6 parts of black garlic juice powder. According to the invention, the eight components are selected and are scientifically combined in an optimal quantity ratio range, and the prepared composition can better reduce the inflammation level of a body, regulate abnormal blood fat, improve the lipid metabolism disorder state and effectively reduce impulsive diet behaviors, so that comprehensive improvement of obesity and cardiovascular related risks is realized, and the health-care effect of a human body is improved. The body is effectively assisted to maintain metabolic health.

The invention belongs to the technical field of nutritional compositions and cosmetics, and particularly relates to an anti-aging composition and application and a product thereof. The composition provided by the invention is composed of L-ergothioneine and alpha-ketoglutaric acid, or L-ergothioneine and alpha-ketoglutaric acid salt, or L-ergothioneine and alpha-ketoglutaric acid salt. Through the synergistic effect of the L-ergothioneine and the alpha-ketoglutaric acid or the alpha-ketoglutarate, the contents of Col I and hydroxyproline in skin tissues can be remarkably increased, the expression of TGF-beta1 is up-regulated and converted, the activity of MMP-1 is inhibited, meanwhile, the malondialdehyde level is reduced to enhance the oxidation resistance, the synthesis of hyaluronic acid is promoted, and the water content of the skin is increased. The composition is simple in component and simple and convenient in preparation process, can be prepared into various products such as cosmetics, health care products or medicines, is used for achieving the effects of resisting aging, moisturizing, resisting inflammation or resisting oxidation, particularly has an excellent improvement effect on skin photoaging induced by ultraviolet rays, and is wide in application prospect.

The invention belongs to the technical field of health-care food, and particularly relates to a composition and oral drops with the effects of dispelling the effects of alcohol, protecting the liver and relieving hangover as well as a preparation method and application of the composition and the oral drops. The invention provides a composition taking silybin eutectic crystal, curcumin eutectic crystal, ampelopsis grossedentata extract, pyrroloquinoline quinone disodium salt, reduced glutathione, alpha-ketoglutaric acid, vitamin, hericium erinaceus extract, frankincense extract, licorice extract and the like as main active ingredients, and the composition is subjected to scientific formula screening and functional verification, and has the advantages that the composition has the obvious curative effect, and the curative effect is obvious; the traditional Chinese medicine composition can effectively improve the metabolism of ethanol, protect liver and gastric mucosa, and is helpful for relieving hangover-related symptoms such as fatigue, thirst, sleepiness, headache, dry mouth, stomachache, nausea, hypomnesis, inattention and the like.

The invention relates to the technical field of medicines, in particular to application of alpha-ketoglutaric acid in preparation of a medicine for preventing and / or treating myopia. The invention provides application of alpha-ketoglutaric acid or pharmaceutically acceptable salt thereof in preparation of products for preventing and / or treating myopia, and the alpha-ketoglutaric acid is taken as an important intermediate metabolite of tricarboxylic acid cycle in mitochondrial aerobic respiration and has oral safety; researches show that alpha-ketoglutaric acid can inhibit myopia progress and ocular axis elongation by improving sclera fibroblast phenotype transformation and extracellular matrix remodeling, and a new idea is provided for preparation of myopia intervention drugs.

The invention aims to disclose a special dietary preparation based on alpha-ketoglutaric acid as well as a preparation process and application thereof, and relates to the technical field of dietary supplements. Every 800 mg of the dietary preparation comprises 50 mg to 100 mg of alpha-ketoglutaric acid or alpha-ketoglutaric acid salt, 5 mg to 12 mg of N-acetylneuraminic acid or N-acetylneuraminic acid salt, 30 mg to 60 mg of ergothioneine, 100 mg to 150 mg of a desert cistanche deserticola water extract or 50 mg to 80 mg of desert cistanche deserticola total glycosides, and auxiliary materials. The alpha-ketoglutaric acid salt is any one or more of alpha-ketoglutaric acid calcium, alpha-ketoglutaric acid sodium and alpha-ketoglutaric acid magnesium; the N-acetylneuraminic acid salt is any one or more of N-acetylneuraminic acid calcium, N-acetylneuraminic acid sodium and N-acetylneuraminic acid magnesium; the special dietary preparation has the beneficial effects that the special dietary preparation simultaneously contains alpha-ketoglutaric acid or alpha-ketoglutaric acid salt, N-acetylneuraminic acid or N-acetylneuraminic acid salt, ergothioneine and the cistanche deserticola aqueous extract, and has the effects of delaying senescence, resisting fatigue, improving the symptom of kidney-yang deficiency and enhancing immunity for males aged more than 45 years old.

There is disclosed a mutant, non-naturally occurring or transgenic tobacco plant or part thereof having modulated expression or activity of lysine ketoglutarate reductase (LKR) comprising, consisting of, or consisting essentially of: (i) a polynucleotide, the present invention relates to a protein comprising, consisting or consisting essentially of a sequence having at least 88% sequence identity to SEQ ID NO: 1 (NtLKR-S) and / or a sequence having at least 86% sequence identity to SEQ ID NO: 3 (NtLKR-T); (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence having at least 89% sequence identity to SEQ ID NO: 2 (NtLKR-S) and / or a sequence having at least 88% sequence identity to SEQ ID NO: 4 (NtLKR-T); or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i) wherein the plant or part thereof comprises at least one modification as compared to a control plant or part thereof wherein the expression of the polynucleotide or the activity of the polypeptide is not modified, the at least one modification is capable of modulating: (a) expression of the polynucleotide in the plant or part thereof; or (b) the activity of the polypeptide in the plant or part thereof.

The invention provides a composition for delaying senescence by activating telomerase activity. The composition is prepared from the following raw materials in parts by weight: 12.5 parts of wheat germ concentrated powder, 0.25 to 5 parts of ergothioneine, 0.25 to 5 parts of pyrroloquinoline quinone disodium salt and 0.25 to 5 parts of alpha-ketoglutaric acid. The invention further provides a preparation method and application of the composition for delaying senescence by activating telomerase activity. Through the synergistic effect of the ergothioneine and the pyrroloquinoline quinone disodium salt, the telomerase activity can be activated, the telomerase expression can be improved, the telomere length can be maintained, and the effect of delaying senescence can be achieved, so that the functional metabolism of a human body can be promoted, and the health state of the body can be improved; through the synergistic effect of the wheat germ concentrated powder and alpha-ketoglutaric acid, cell aging can be delayed; through the multi-target synergistic effect of'telomerase activation + mitochondrial repair + autophagy enhancement ', the anti-aging effect is delayed, the metabolic capability is high, the anti-aging effect is good, and safety and high efficiency are achieved; belongs to the technical field of food and health care product processing.

The invention discloses an application of alpha-ketoglutaric acid (alpha-KG) in preparation of a feed additive for relieving skeletal muscle development injury of pregnancy heat stress induced offspring calves. According to the additive, sodium alpha-ketoglutarate (C5H4O5Na2) accounting for 0.1% of dry matter is uniformly mixed into dry dairy cow total mixed ration (TMR), and the dairy cow is fed with heat stress (THIgt; 72) pregnant Holstein cows, the weight of fetal cattle is increased by 23.2%, and the weight of skeletal muscles is increased by 31.3%. Meanwhile, during pregnancy, alpha-KG is fed, so that the weight of the offspring weaned calves is increased by 10.5%, the weight of skeletal muscles is increased by 28.6%, and the fiber density of the skeletal muscles is improved. According to the method, the skeletal muscle growth and fiber density of the offspring calves are subjected to targeted cross-generation regulation and control through maternal nutrition intervention for the first time, the regulation and control bottleneck of heat stress cross-generation injury in the prior art is broken through, and a solution is provided for improving animal health and growth potential for ruminant heat stress management.

The invention discloses an application of alpha-ketoglutaric acid in preparation of a medicine for promoting hypertension angiogenesis, belongs to the technical field of biological medicines, and finds that the alpha-ketoglutaric acid has the effects of promoting hypertension angiogenesis and improving microcirculation injury for the first time, and provides a new thought for diagnosis and treatment of sparse microcirculation. Alpha-ketoglutaric acid has good drug safety and a strong angiogenesis promoting effect, and can be used as a candidate drug for promoting angiogenesis. And the DM-alpha KG is alpha-ketoglutaric acid which can be absorbed by cells, can be dissolved in DMSO, and is good in stability, low in cytotoxicity and good in drug safety. Results show that the alpha-ketoglutaric acid has relatively good drug safety and relatively strong angiogenesis promoting effect, and can be used as a candidate drug for improving sparse blood vessels.

There is provided a method for diagnosing a subject with early onset muscle atrophy, said method comprising: obtaining a biosample from the subject; and assaying the biosample for one or more biomarkers, said one more biomarkers are taurine, proline, citrulline, trigonelline, thymidine, ornithine, glutamate, L-pyroglutamic acid, creatinine, adenine, nicotinamide, 2-methylhippuric acid, maltol, L-arginine, hypotaurine, L-glutamine, homogentisic acid, methylhistidine, oxoglutaric acid, xanthine, L-carnitine, succinate, or a combination thereof; and identifying the subject with early onset muscle atrophy on the basis of a deviation in the one or more of said one more biomarkers.

The invention discloses a low-foam acidic zinc plating additive and a preparation process thereof, and belongs to the technical field of electroplating additives, the additive comprises a low-foam softening agent and a water-based brightener, and the low-foam softening agent comprises the following raw materials in percentage by mass: 0.8-1.2% of polyethyleneimine, 0.03-0.06% of monododecyl phosphate potassium, 0.2-0.6% of water-soluble polyether amine and the balance of water. The water-based brightener is prepared from the following raw materials: 0.6 to 1.2 percent of 5-hydroxymethylfurfural, 0.2 to 0.4 percent of L-cysteine salt, 0.1 to 0.15 percent of 4-amino-2 (1H) pyrimidone, 0.05 to 0.1 percent of sodium salicylate, 1 to 1.5 percent of citric acid, 0.3 to 0.5 percent of alpha-ketoglutaric acid, 0.2 to 0.4 percent of erythorbic acid and the balance of water. The water-based formula is prepared by optimizing the components in the additive, the components are uniformly dispersed, the foam amount is low, and the plating quality is good.

The present invention relates to the technical field of anti-aging compositions and particularly to an anti-aging composition and use thereof, and an anti-aging capsule and use thereof. The present invention provides an anti-aging composition comprising the following components: 20-2000 parts by mass of calcium α-ketoglutarate, 20-100 parts by mass of quercetin, 20-2000 parts by mass of trans-resveratrol, 20-100 parts by mass of calendulin, and 0.02-1.8 parts by mass of spermidine. The composition of the present invention has a multi-target synergistic effect on 12 major causes of aging and can safely and effectively reverse aging, enhance the physiological function, reduce the risk of chronic diseases, and improve the quality of life. The components of the composition are safe, and are carefully selected natural food ingredients; therefore, the composition is safe and reliable, and has no side effects with long-term use. The composition is prepared in an oral dosage form, so that the composition is convenient to administer, which improves patient compliance; moreover, the mode of application of the composition can be expanded.

The invention discloses a pharmaceutical composition for delaying oocyte senescence and application thereof, and relates to the technical field of biological medicine, and the pharmaceutical composition comprises alpha-ketoglutaric acid, a bicarbonate buffer solution, an energy metabolism substrate, polyvinyl alcohol, hormone, a growth factor and insulin-transferrin-selenium. The pharmaceutical composition for delaying oocyte aging disclosed by the invention is beneficial to improving the maturation rate of oocytes, reducing the cytoplasm fragmentation rate and the cleavage fragmentation rate, and improving the cleavage rate and the blastocyst rate; according to the invention, the content of beta-galactosidase is reduced, the function of mitochondria in the aging process after ovulation of the mature oocyte is improved, the membrane potential of the mitochondria in the aging process after ovulation of the mature oocyte is stabilized, the activity of the mitochondria is improved, and the normalization of RNA methylation modification in the aging process after ovulation of the mature oocyte is promoted.

The use of α-ketoglutaric acid in the preparation of a drug for preventing and treating demyelination-related diseases. The α-ketoglutaric acid or a derivative thereof is used for preventing and / or treating myelin sheath defects in demyelination-related diseases, such as demyelinating diseases, amyotrophic lateral sclerosis, Huntington's disease, hypomyelinating leukodystrophy, Alzheimer's disease, Parkinson's syndrome and diabetes-related visual impairment, and can promote myelin sheath generation, regeneration or repair, and improve the immune microenvironment in the lesion area.

The invention discloses a method for promoting biomass accumulation of haematococcus pluvialis, which comprises the following steps: adding salicylic acid, melatonin and alpha-ketoglutaric acid into a basic culture medium, and replacing ferric trichloride with ferric citrate as an iron source to obtain an improved BG-11 culture medium; the method comprises the following steps: inoculating a haematococcus pluvialis seed solution into a culture bottle containing an improved culture medium, carrying out illumination treatment on the first day to the second day by adopting pulsed red light with the wavelength of 620 nm in the early stage of a green stage, carrying out illumination treatment on the third day to the fifth day by adopting red light with the wavelength of 660 nm in the middle stage of the green stage, and carrying out illumination treatment on the sixth day to the seventh day by adopting pulsed red light with the wavelength of 680 nm in the later stage of the green stage. Through a synergistic scheme of the improved BG-11 culture medium, staged red light treatment and dynamic environment regulation and control, the problems of low light energy utilization rate and insufficient stress resistance in the existing haematococcus pluvialis culture process are solved, high efficiency and stability of haematococcus pluvialis biomass accumulation are realized, the biomass can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage, and the yield of the haematococcus pluvialis can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage. And the survival rate of haematococcus pluvialis cells reaches 95%.

The invention relates to the technical field of natural product extraction, and particularly discloses a method for extracting astaxanthin from haematococcus pluvialis. According to the method, a deep eutectic solvent (DES) composed of sodium alpha-ketoglutarate and lactic acid is adopted as an extraction medium, the molar ratio of the sodium alpha-ketoglutarate to the lactic acid is 1: 1-1: 4, water bath ultrasonic extraction is carried out at the temperature of 25-50 DEG C, and the liquid-solid ratio is 10: 1-40: 1. Experiments show that under the optimal condition, the astaxanthin extraction rate and the DPPH free radical scavenging rate are obviously superior to those of a traditional solvent method. The method has the characteristics of greenness, high efficiency and simplicity and convenience in operation, and provides a new strategy for astaxanthin extraction.

The invention relates to the field of synthetic biology, metabolic engineering and fermentation engineering, and discloses a genetically engineered bacterium and application thereof in production of alpha-ketoglutaric acid.The genetically engineered bacterium comprises the steps that the genetically engineered strain with escherichia coli BW25113 or corynebacterium glutamicum ATCC13032 as a host is constructed; overexpression type isocitrate dehydrogenase genes are integrated through chromosomes, alpha-ketoglutarate dehydrogenase complex genes are knocked out, NADPH invertase is overexpressed through plasmids, and exogenous AKG transporter protein is introduced, so that metabolic flux directional regulation and control and product efflux enhancement are realized; meanwhile, a staged fermentation process is adopted and comprises dynamic gradient adjustment of inorganic phosphorus concentration and two-stage pH control. According to the method, the AKG yield of 78.2 g / L can be obtained under the 5L tank scale, the sugar acid conversion rate reaches 0.68, and the method has the advantages of being high in yield, low in byproduct and controllable in cost. The obtained AKG is suitable for a plurality of fields such as medical intermediates, functional foods and cell culture additives, and has wide industrial application prospects.

The invention relates to the technical field of tissue engineering material preparation, and discloses a gelatin chitosan-based hydrogel preparation method and application thereof, and the gelatin chitosan-based hydrogel preparation method comprises the following steps: dissolving chitosan in an aqueous solution containing alpha-ketoglutaric acid and protocatechuic acid to prepare a chitosan solution; in the process, alpha-ketoglutaric acid reacts with amino of chitosan, and meanwhile, alpha-ketoglutaric acid and protocatechuic acid act synergistically to form a basic reaction system of the solution. The preparation method comprises the following steps: adding gelatin into a chitosan solution, so that chitosan, alpha-ketoglutaric acid, protocatechuic acid and gelatin in the solution are fully mixed through blending to form a uniform pre-reaction system; the mixed solution is placed under a proper temperature condition for incubation, in the process, a series of non-covalent bond effects and local chemical bond effects occur in the system, and the chitosan and the gelatin form a stable network structure. After incubation is completed, the solution is gradually converted into gel with excellent performance. And the finally prepared hydrogel has good biocompatibility and temperature-sensitive characteristics.

The present invention relates to a precise lactation feed for lactating sows, and 100 kg of the feed product comprises the following raw materials by weight: 20 to 28 kg of soybean meal, 2 to 5 kg of fish meal, 2 to 5 kg of MCT microcapsules, 0.2 to 0.4 kg of rumen protected methionine, 0.1 to 0.2 kg of rumen protected tryptophan, 1.5 to 2.5 kg of inulin, 1 to 2 kg of sodium lignin sulfonate, 0.01 to 0.03 kg of a fenugreek seed extract, 1.2 to 1.8 kg of sn-2 palmitate, 0.4 to 0.6 kg of alpha-ketoglutaric acid, 0.08 to 0.12 kg of glycine iron, and the balance of water. The feed is prepared from the following raw materials by weight: 0.15-0.25 kg of zinc methionine, 4-8 kg of wheat bran, 1.2-1.8 kg of a calcium propionate solution, 2-3 kg of lactic acid bacteria fermentation liquor, 0.2-0.4 kg of a rosemary extract and the balance of corn, wherein the total weight of the raw materials is 100 kg. The feed is a precise nutrition regulation feed formula for the sows in the lactation period, and milk yield increase, piglet weaning performance optimization and sow body condition protection are achieved through multi-dimensional nutrition intervention.

The invention relates to an asymmetric bioadhesive patch with controllable modulus adhesion as well as a preparation method and application of the asymmetric bioadhesive patch. The asymmetric bioadhesive patch is of a double-layer composite gel structure and comprises an elastomer layer used for being adhered to a medical instrument and a hydrogel layer used for being adhered to soft tissue. The elastomer layer is prepared from the following raw material components: hexadecyl acrylate, a long-chain cross-linking agent and a photoinitiator; the raw materials of the hydrogel layer comprise any one of the following two groups of raw material components: acrylic acid, gelatin, acrylic acid N-hydroxysuccinimide ester, methacrylated gelatin, alpha-ketoglutaric acid and the balance of water; or acrylic acid, acrylic acid N-hydroxysuccinimide ester, N, N '-methylene bisacrylamide, alpha-ketoglutaric acid and the balance of water. Compared with the prior art, the two sides of the asymmetric bioadhesive patch can be adhered to medical equipment and soft tissue respectively, so that the medical equipment can be effectively fixed in the soft tissue.

The invention relates to the technical field of biological medicines, and discloses an alpha-ketoglutaric acid composite liposome as well as a preparation method and application thereof. Alpha-ketoglutaric acid is preliminarily entrapped by lecithin and cholesterol to prepare a basic liposome, and then enzymolysis silkworm pupa protein is introduced to the surface of the liposome, so that the enzymolysis silkworm pupa protein and a lipid bilayer are subjected to hydrophobic and hydrogen-bond interaction to form a protein-lipid composite layer, and the stability of a membrane structure is enhanced; and the positively charged arginine modified starch is further deposited on the surface of the liposome to form a polysaccharide-protein-lipid composite layer, so that the electrostatic locking of the negatively charged alpha-ketoglutaric acid is realized, and the encapsulation efficiency and the controlled release performance are further improved. The alpha-ketoglutaric acid composite liposome provided by the invention has the advantages of being high in stability, safe to eat, high in encapsulation efficiency, good in gastric acid resistance stability and the like, has a good slow release characteristic, and can be applied to the fields of sports nutritional supplements, antioxidant products or anti-aging products and the like.

The invention provides a method for improving fermentation yield and sugar-acid conversion rate of 5-hydroxytryptophan, alpha-ketoglutaric acid ferrous salt, alpha-ketoglutaric acid zinc salt, alpha-ketoglutaric acid cobalt salt and alpha-ketoglutaric acid manganese salt are added into a fermentation culture medium according to a specific proportion, alpha-ketoglutaric acid directly participates in TCA circulation, circulation operation can be accelerated, generation of NADH and FADH2 is increased, the yield of 5-hydroxytryptophan is increased, and the yield of 5-hydroxytryptophan is increased. The ATP synthesis efficiency is improved, and more energy is provided for cell growth; the alpha-ketoglutaric acid can balance amino acids required in the growth process of escherichia coli, promote the generation of proteins and nucleic acids and accelerate the growth of thalli; the alpha-ketoglutaric acid metal salt can improve the utilization efficiency of microelements by thalli; ferrous ions are cofactors of 5-hydroxytryptophan hydroxylase and can accelerate the hydroxylation process of tryptophan. Through the synergistic effect of the added components, the yield of the 5-hydroxytryptophan and the sugar-acid conversion rate are improved.

The invention discloses a saccharomyces cerevisiae engineering bacterium for producing heme-binding protein as well as a construction method and application of the saccharomyces cerevisiae engineering bacterium. The construction method comprises the following steps: (1) by taking Saccharomyces cerevisiae CEN.PK2-1C as an original strain, optimizing a metabolic pathway, enhancing the supply of cytoplasm alpha-ketoglutaric acid (alpha-KG) and succinyl coenzyme A, and realizing the efficient accumulation of 5-aminolevulinic acid (ALA); (2) regulating and controlling the heme synthesis gene cluster in a time-sharing manner, and cooperatively expressing heme binding protein, so as to improve the assembly efficiency of heme (Heme); and (3) a secretion system is optimized, the secretion bottleneck of eukaryotic protein is broken through, and transmembrane transport and extracellular accumulation of the heme-binding protein are realized. Through the transformation of the combination strategy, an engineering strain for efficiently producing the heme-binding protein is obtained, and the yield of the bean hemoglobin in the saccharomyces cerevisiae is remarkably improved. Under the fermentation condition of a 5L fermentation tank, the secretory expression quantity of the bean hemoglobin reaches 286.6 mg / L.