5 results about "Amidinotransferases" patented technology

The invention discloses an aminotransferase mutant from thermophilus thermophilus and application of the aminotransferase mutant in production of L-leucine, the activity of the aminotransferase of the mutant is high, the yield of the L-leucine is increased, and meanwhile, accumulation of a byproduct valine is effectively reduced.

The present application relates to the technical field of enzyme engineering, and discloses an amino transferase mutant and application thereof.The present application provides a wild-type amino transferase mutant derived from Aspergillus avenaceus.The amino transferase mutant is obtained by carrying out single-point or multi-point combined mutation on the 20th, 60th, 92nd and 186th positions of the amino acid sequence shown in SEQ ID NO.2, and can directly take the sitagliptin intermediate precursor ketone as a substrate, take isopropylamine as an amino donor, take phosphopyridoxyl as a coenzyme, take dimethyl sulfoxide as a substrate solvent, catalyze the preparation of a sitagliptin intermediate with high optical purity, and the specific enzyme activity reaches 139.3 U / g in a reaction system with a final concentration of 50% DMSO, greatly improves the catalytic efficiency, and the product has high stereoselectivity (the e.e. value of the product reaches 99%), and has a wide industrial application prospect.

The application discloses a high-yield L-homoserine Corynebacterium glutamicum mutant strain and application thereof, and belongs to the technical field of fermentation engineering. The application takes Corynebacterium glutamicum ATCC13032 as a starting strain, knocks out the regulatory protein McbR, amino acid intracellular transport protein MetD, homoserine kinase, phosphoenolpyruvate carboxykinase, cysteine beta cleavage enzyme, O-acetylhomoserine sulfhydrylase, weakens isocitric acid dehydrogenase and dihydrodipicolinic acid synthase, and overexpresses pyruvate carboxylase gene mutant, aspartate kinase mutant, aspartate semialdehyde dehydrogenase, homoserine dehydrogenase, branched-chain amino acid transport protein BrnF, branched-chain amino acid transport protein BrnE, aspartate aminotransferase, bifunctional aspartate kinase / homoserine dehydrogenase 1 mutant, NADP + dependent glycerol-3-phosphate dehydrogenase. The recombinant bacteria provided by the application can reach 63.5g / L of L-homoserine yield after 96h of culture in a fermenter.

The invention provides an aminotransferase and an application of the aminotransferase in preparation of a chiral cyclic amide compound. The invention specifically provides an aminotransferase mutant, and compared with an amino acid sequence as shown in SEQ ID NO: 1, the aminotransferase mutant contains the difference of amino acid residues at one or more sites. The aminotransferase provided by the invention is high in catalytic yield, good in selectivity and good in application prospect.

The application relates to the field of biological medicine, and discloses a use of indole-3-propionic acid in preparation of a medicine for preventing and treating acute-on-chronic liver failure. Experiments prove that the indole-3-propionic acid can effectively inhibit the formation of neutrophil-related extracellular traps, reduce the levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin and inflammatory factors TNF-alpha, IL-6 and IL-1beta related to acute-on-chronic liver failure, reduce liver tissue necrosis and inflammatory infiltration, and improve the survival rate. Therefore, the indole-3-propionic acid can be used for preparing a medicine for preventing and / or treating acute-on-chronic liver failure, and provides a new small-molecule intervention strategy for acute-on-chronic liver failure.