30 results about "Glutamate kinase" patented technology

The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and / or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic N-acetyl glutamate kinase (NAGK) gene that modulates nitrogen use in plants. The invention also includes the overexpression and enzymatic characterization of an arginine-insensitive NAGK isolated from a bacterial strain that improves stress tolerance and nitrogen uptake, metabolism or both. In various embodiments, the vector construct includes one or more nucleic acid sequences including SEQ ID NO: 1. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.

The invention provides a Corynebacterium glutamicum for producing L-proline, and a method for producing L-proline by using the strain. The L-proline-producing Corynebacterium glutamicum constructed by the present invention, wherein proline dehydrogenase / pyrrole-5-carboxylate dehydrogenase PutA is inactivated, glutamate kinase ProB, glutamate-5- Enhanced activity of semialdehyde dehydrogenase ProA, pyrrole-5-carboxylate dehydrogenase ProC, pyruvate carboxylase Pyc, glyceraldehyde-3-phosphate dehydrogenase GapN, L-proline efflux protein ThrE or SerE, The L-glutamate efflux protein MscCG is inactivated, and the L-proline yield, transformation rate and production intensity of the obtained strain are significantly improved compared with the starting strain, which can reduce the production cost of L-proline.

The invention relates to a method for improving the yield of arginine by the mutation of Corynebacterium crenatum N-acetyl glutamic acid kinase. L-arginine is one of semi-essential basic amino acids for a human body, and has various particular physiology and pharmacology functions. High-yield arginine mutant strain C. crenatum SYPA5-5 is compounded into the arginine through a circulating way, andN-acetyl glutamic acid kinase (NAGK) is a key enzyme in the compounding way and is subject to the feedback inhibition of the product arginine. By using an overlapping PCR (Polymerase Chain Reaction) technique, a GAT (glycerol phosphate acyl transferase) codon used for coding aspartic acid is used for substituting a GGA (General Gonadotropic activity) codon used for coding glycine at the site 287 in the coding NAGK albumen, and the NAGK which has high activity and arginine with obviously reduced by the feedback inhibition can be obtained after the mutation. An argBSD gene is brought into the Corynebacterium crenatum of the high-yield arginine through pJCl-tac, and the expression volume of the key enzyme is further improved. The final acid yield is increased to 36.3g / L from original 28g / L, and the yield of the L-arginine is increased by 29.7 percent.

The invention provides an N-acetyl glutamic acid kinase mutant capable of relieving the feedback inhibition effect of L-arginine and obviously increasing the catalytic activity and thermal stability and belongs to the field of bioengineering. A molecular docking technique and the structure analysis for N-acetyl glutamic acid kinase (CcNAGK) from corynebacterium crenatum CGMCC NO:0890 are firstly adopted for confirming that the E19 locus plays an important role in inhibiting the feedback of arginine and three key amino residue loci I74, F91 and K234 located on a substrate combining bag have significant impacts on enzymatic catalytic activity and heat stability; the encoded amino acid is replaced and the gene argB4 after compound mutation is introduced into the corynebacterium crenatum highly yielding arginine through pDXW10, so that the compounding rate of the arginine in corynebacterium crenatum can be obviously increased; lastly, after fermentation for 98h in a 5L fermentation tank, the yield of arginine is increased to 61.2g / L from original 43.2g / L, and the yield of the L-arginine is increased by 41.8%.