42 results about "Cyclic lipopeptide" patented technology

The present invention discloses a kind of antitumor, antiviral and antibacterial active cyclopeptide compound. The fermented liquid of Bacillus sp., which is marine bacteria collected from deposited marine mud, as the material is separated via solvent extraction and column chromatography to obtain one kind of new cyclic lipopeptide compound. The chemical structure of the compound is determined by means of high resolution mass spectral, nuclear magnetic resonance and other spectral technology. The compound is expected to become medicine for treating cancer, and Staphylococcus aureus and virus inflection.

Compositions and methods for application to plants are provided for a new strain of Bacillus licheniformis RTI184 having plant growth promoting activity. Compositions and extracts of Bacillus licheniformis strains that include newly identified Fengycin-like and Dehydroxyfengycin-like cyclic lipopeptides designated as “Fengycin MB-Cit” and “Dehydroxyfengycin MB-Cit”, respectively, are also provided. In particular aspects, compositions containing the Bacillus licheniformis strain RTI184 can be applied alone or in combination with chemical agents or other microbial agents to benefit plant growth.

The present invention belongs to the field of a biological technique and relates to a RNA polymerase mutant of Bacillus spp CC09 bacterial strain of high-yield antimycotics cyclic lipopeptides substances screened by using a LB plate containing rifampin (50 mug / ml). The mutation site of the mutant is on any position of 485-490 in a RNA polymerase beta sub-unit amino acid sequence, Compared with wild type CC09 bacterial strain, the output of antimycotics cyclic lipopeptides antibiotics Iturin and Surfactin produced by the mutant can be increased by 100%-300%, the antifungal activity is increased by 20-60%, and the mutant can be used for preventing and treating a plurality of crop diseases.

The invention relates to the use of one or more cyclic lipopeptides, such as surfactins A, B1 and C and derivatives and mixtures thereof, as a taste modulator and / or sweetness enhancer for comestible compositions containing at least one natural or artificial sweetener. The comestible compositions include food, beverages, medicinal products and cosmetics and contain preferably mono-, di- or oligosaccharides as sweeteners. The invention further relates to said comestible compositions containing a cyclic lipopeptide as taste modulator.

Compositions are provided that are planting matrices containing spores of Bacillus spp. microorganisms for benefiting plant growth. The Bacillus spp. microorganisms include newly identified Bacillus licheniformis strain RTI184 deposited as ATCC No. PTA-121722 and Bacillus licheniformis CH200 deposited as Accession No. DSM 17236. Both Bacillus licheniformis strains were shown to produce previously unreported Fengycin-like and Dehydroxyfengycin-like cyclic lipopeptides that are not produced uniformly among strains of Bacillus licheniformis. The planting matrices provided include potting soils. The pH of the potting soils can range between 4 to 8. The growth benefit provided by the planting matrices can be exhibited by improved seedling vigor, improved root development, improved plant growth, improved plant health, increased yield, and improved plant appearance.

The invention discloses a biosynthetic gene cluster of cyclic lipopeptide compounds, a method for activating the biosynthetic gene cluster and application thereof. Nucleotide sequences of the biosynthetic gene cluster of the cyclic lipopeptide compounds Totopotensamides are shown as 2972nd-101763rd bit nucleotide sequences in SEQ ID NO.1. The biosynthetic gene cluster, the method and the application have the advantages that all gene and protein information related to the biosynthetic gene cluster of the cyclic lipopeptide compounds Totopotensamides can assist people to understand biosynthesismechanisms of the cyclic lipopeptide compounds Totopotensamides, and materials and knowledge can be increased for further genetic modification; bases can be provided for activating expression of silent gene clusters by means of controlling regulatory genes, natural product biosynthetic gene cluster banks can be enriched, and entity can be provided for discovering lead compounds of medicines.

The invention relates to the field of improvement of and planting with desert soil, in particular to a slow-release soil conditioner suitable for planting with desert soil and application of the slow-release soil conditioner. The slow-release soil conditioner in the invention comprises the following components in percentage by mass: 30 to 60% of natural peat soil, 3.0 to 10% of wormcast powder, 20to 40% of mineral-derived humus, 5.0 to 20% of urea, 1.0 to 5.0% of cyclic lipopeptide, 0.5 to 1.5% of a chelating agent and 1.0 to 10% of microorganism compound zymophyte powder. In the process of improving the wettability of the humic acid-containing soil conditioner, the problems of water shortage and lack of water in a medium desert soil environment are solved, the fertility of soil is improved, and the conditioned soil is suitable for wheat planting.

The invention relates to a group of new cyclic lipopeptides of locillomycins A, B and C and a preparation method thereof, a medicinal composition with active ingredients of locillomycins A, B and C, and an application of the locillomycins A, B and C in preparation of antifungal, antibacterial and antiviral medicaments. The locillomycins A, B and C are new cyclic lipopeptides separated from a culture of Bacillus subtilis Bs916 strains, and a result of an in vitro activity test proves that the locillomycins A, B and C have very strong inhibitory activity to pathogenic fungi, bacteria and viruses, are used as a new antifungal, antibacterial and antiviral medicament and have a good development prospect.

The invention discloses a fermentation medium which comprises 0.5-5.0 wt% of amino acid or its derivative, 0.5-3.0 wt% of hard-soluble organic nitrogen source, and 1.0-10.0 wt% of carbon source. By using the medium of the invention to carry out biologic fermentation of the compound of formula I, the fermentation level can stably reach more than 0.5g / L, with the highest fermentation level being 1.0g / L. By using the strain mutagenized with Coleophoma empetri F-11899 (FERM BP2635), the fermentation level can even reach 1.5 g / L. Because the fermentation level is high and the amount of organic solvent used in the post-treatment is relatively reduced, the production cost is reduced, and the invention has high economic value and meets the need of industrial production.

A process for purifying cyclic lipopeptide compounds or salts thereof comprising the steps of: (1) charging a crude compound of Formula I onto a macroporous adsorption resin; (2) washing the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as a washing liquid; and (3) eluting the compound of Formula I from the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as an eluent. The purification method has the advantages of using a small amount of organic solvents, using no silica gel, and causing little damage to the environment; the purity of the collected compound of formula I is also improved as compared with the methods previously disclosed.

The objective of the present invention is to provide an inorganic nanoparticle dispersion liquid in which an amount of a surfactant is reduced or in which an inorganic nanoparticle is dispersed with improved dispersion stability, and a production method thereof. The method for producing an inorganic nanoparticle dispersion liquid according to the present invention is characterized in comprising the steps of mixing an inorganic nanoparticle, a cyclic lipopeptide biosurfactant and a medium, and dispersing the inorganic nanoparticle in the medium.

The present invention relates to a method for producing a cyclic peptide compound or a salt thereof which comprises contacting a cyclic lipopeptide compound or a salt thereof with an acylase derived from a microorganism belonging to the genus Pseudomonas to deacylate the lipid acyl moiety of said cyclic lipopeptide.

The invention belongs to the technical field of agricultural fertilizer and discloses a loss-controlling fertilizer special for corn and a preparation method thereof. A chemical fertilizer nutrient loss-controlling agent added in the invention is a physical and chemical modified nanometer material-attapulgite crystal; the attapulgite crystal reacts with the fertilizer molecule through hydrogen bond, van der waals force and viscous force, so as to form a micro-nano interpenetrating polymer network and absorb and capture the nutrients, such as, nitrogen, phosphor and potassium in the chemical fertilizer; the chemical fertilizer nutrient loss-controlling agent contains cyclic lipopeptide biosurfactant and compound amino acid thereof and is capable of promoting the absorption of the corn for the fertilizer nutrient and achieving the effects of controlling nutrient loss and increasing the use ratio of the chemical fertilizer; the loss-controlling fertilizer prepared according to the method is uniform in nutrient, is stable and long-lasting in fertilizer effect release, can be fertilized at one time, requires no topdressing and has obvious effect for increasing yield and income; the loss-controlling fertilizer has the advantages of improving and fertilizing soil and is capable of reducing the chemical fertilizer loss and the pollution to environment.

The invention discloses a preparation method of immobilized cyclic lipopeptide deacylase. A cyclic lipopeptide deacylase solution is directly mixed with a porous hydrophily enzyme carrier without purification, one or more kinds of organic solutions and inorganic salt are added, and then the immobilized cyclic lipopeptide deacylase is obtained. The invention further discloses application of the method in industrial production of micafungin parent nucleuses and anidulafungin parent nucleuses. The preparation method is simple in technology, enzyme purification equipment is omitted, the recovery rate of enzyme activities is obviously increased in the immobilization process, and immobilized enzymes can be repeatedly used. The enzyme method for converting micafungin and anidulafungin intermediates has higher concentration of reaction substrates when compared with a fermentation conversion method, a few product impurities are generated, and the conversion efficiency is high.

Compositions and methods for application to plants are provided for a new strain of Bacillus licheniformis RTI184 having plant growth promoting activity. Compositions and extracts of Bacillus licheniformis strains that include newly identified Fengycin-like and Dehydroxyfengycin-like cyclic lipopeptides designated as “Fengycin MB-Cit” and “Dehydroxyfengycin MB-Cit”, respectively, are also provided. In particular aspects, compositions containing the Bacillus licheniformis strain RTI184 can be applied alone or in combination with chemical agents or other microbial agents to benefit plant growth.

The invention relates to the field of improvement of and planting with aeolian sandy soil, in particular to a slow-release water-soluble fertilizer suitable for planting with aeolian sandy soil and application of the slow-release water-soluble fertilizer. The slow-release water-soluble fertilizer suitable for planting with aeolian sandy soil in the invention comprises the following components in percentage by mass: 55 to 75% of mineral-derived humus, 5.0 to 20% of urea, 2.0 to 8.0% of diammonium phosphate, 5.0 to 15% of monopotassium phosphate, 1.0 to 5.0% of cyclic lipopeptide and 0.5 to 1.5%of a chelating agent. In the process of improving the wettability of the humic acid-containing water-soluble fertilizer, the problems of water shortage and lack of water in a medium aeolian sandy soil environment are solved, soil fertility is improved, and the improved aeolian sandy soil is suitable for potato planting.

The objective of the present invention is to provide a cleaning agent composition which can be used in the open air without any problems since the cleaning agent is environmentally friendly and which is effective for removing a low-molecular-weight and poorly-water-soluble cyclic silicone compound that causes a stain of a silicone sealing. The cleaning agent for washing out a silicone stain according to the present invention is characterized in comprising a cyclic lipopeptide biosurfactant, wherein the specific silicone stain compound such as D4 compound is the objective compound to be removed.

The invention discloses a method for biosynthesis of acyclic lipopeptide surfactant. The method comprises the steps of first taking seawater with additional ammonium chloride as a culture medium, and performing staphylococcus WX2701 low-temperature anaerobic fermentation; performing sterile filtration on obtained fermentation broth, and sequentially adding achromobacter WX1501 and long-chain aliphatic hydrocarbon (C20-40) monomers or mixture into obtained filtrate to perform fermentation; and obtaining the cyclic lipopeptide surfactant having high surface activity through separation from the fermentation broth. According to the method, two-step microorganism total synthesis of the lipopeptide surfactant is adopted, mutual competition between microorganism strains is effectively avoided, a metabolite inhibiting effect is greatly reduced, the yield of the lipopeptide surfactant reaches 38.7 g / L, the yield of the surfactant produced by single bacterium fermentation is greatly improved, and the industrialization of the lipopeptide surfactant is expected to be achieved.

The invention provides a method for preparing antifungal cyclic lipopeptide echinocandin B. The method comprises the following steps: 1) performing solid-liquid separation on an ECB (Echinocandin) fermentation broth; 2) enriching with a macroporous adsorption resin ADS-17 or X-5; 3) eluting the macroporous adsorption resin with hot absolute methyl alcohol, and combining eluants; 4) concentrating the eluants, adding a sodium chloride solution, stirring, cooling for crystal separation, and drying crude ECB filter cakes; 5) filtering the crude ECB filter cakes with methanol, and treating filtratewith a reverse phase resin column XT30; 6) concentrating the reverse phase resin column XT30 eluant, adding the sodium chloride solution, stirring, cooling for crystal separation, and drying ECB filter cakes; 7) collecting a dried ECB product. By adopting the method, the problems that byproducts are hard to elute and solvents are wasted are simply and effectively solved. Solid-liquid separation of water-containing ECB can be effectively completed, and product degradation is remarkably reduced. The method is simple and feasible and is applicable to industrial production, and the final purity of the echinocandin B is improved to 99% or greater.

The invention relates to a cyclic lipopeptide derivative and preparation and application thereof. The cyclic lipopeptide derivative is a compound with a structure as shown in a formula A, wherein A1,A2, A3, A4, A5, A6, A7 and A8 are all amino acids, and every two of the A1, A2, A3, A4, A5, A6, A7 and A8 are connected through an amido bond. The A1 is L-Asn; the A2 is L-Tyr or D-Tyr; the A3 is L-Asn or D-Asn; the A4 is L-Ser or D-Ser; the A5 is L-Asn or D-Asn; the A6 is L-Gln; the A7 is L-Pro; and the A8 is L-Cys, and a hydrogen atom on -SH of L-Cys is substituted by one of the following groups: a C1-C18 alkyl group, C4-C11 carboxylic acid, a C4-C11 alkyl cyano group, and a C4-C11 carboxylic acid methyl ester. The cyclic lipopeptide derivative has an excellent antibacterial effect.

Cyclic lipopeptides having one or more modifications relative to daptomycin, and methods of making them are provided. The cyclic lipopeptides can be used as antibacterial agents. The daptomycin analogues are generated by chemical synthesis, which makes introduction of an unnatural amino acid and any modification into daptomycin possible. Pharmaceutical compositions and method of use thereof for the disclosed daptomycin analogues are also provided.

A method for purifying a cyclic lipopeptide or a salt thereof is provided. The method comprises the steps: (1) extracting a fermentation broth containing a compound of formula I or a salt thereof, to obtain an extract 1 after filtration or centrifugation; (2) diluting or concentrating the extract 1 under vacuum to decrease the content of the organic solvent, to obtain an extract 2; (3) loading the extract 2 onto a macroporous absorption resin; (4) washing the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution; and (5) eluting the compound of formula I off from the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution as an eluant. Compared with the prior art, the purification method has the advantages that fewer organic solvent is used, no silica gel is used, the harm to the environment is less, and the purity of the collected compound of formula I is improved.

The present invention discloses a cyclic lipopeptide nutritional chewable tablet and a preparation method thereof, and belongs to the technical field of food processing. The cyclic lipopeptide nutritional chewable tablet comprises the following ingredients in parts by mass: 10-30 parts of cyclic lipopeptide powder, 30-80 parts of nutrients, 5-15 parts of a flavoring agent, 1-5 parts of a binder and 0.3-1 part of a lubricant. The nutrients are skim milk powder and dextrin (at a mass percentage of 2:1 to 5:1). The preparation method comprises the following steps: the cyclic lipopeptide powder used as a main functional ingredient and the other auxiliary materials are prepared according to a certain ratio; and soft material preparing, granulating, tableting and sterilizing are conducted to prepare the cyclic lipopeptide nutritional chewable tablet. The chewable tablet is comprehensive in the nutrients, has multiple health-care effects and is convenient to eat and carry, sour, sweet, tastyand suitable for various people.

The invention belongs to the field of pharmacy, discloses an application of cyclic lipopeptide Fengycin in preparation of an antineoplastic drug, and provides a novel application of cyclic lipopeptide Fengycin in preparation of an antineoplastic drug, in particular to an application in preparation of drugs for treating lung cancer, stomach cancer, breast cancer and colonic cancer. The cyclic lipopeptide Fengycin has an obvious inhibiting effect on a tumor cell and a tumor tissue, has a better selectivity to the tumor cell and the tumor tissue, and has an obvious influence on associated protein related with tumor apoptosis. The application of the cyclic lipopeptide Fengycin in preparation of the antineoplastic drug provides an evidence for researching novel antineoplastic drugs and has a good clinical application prospect.

The method for removing lipopeptides from solutions and changing their structure from cyclic to linear, characterised in that lipopeptide capture and sequestration takes place from solutions containing water through sorption, and in the case of cyclic lipopeptides, in which the peptide ring is closed by a lactone bond, sorption is additionally associated with hydrolysis reactions occurring on thesurface of active carbon, leading to linearisation as a result of breaking the lactone bond.

Methods are provided for producing a gelatinous composition containing a phospholipid, which has excellent emulsion stability, as well as methods for easily producing an emulsified composition that is excellent in use feeling and emulsion stability. Gelatinous compositions having excellent emulsion stability, and emulsified compositions having excellent use feeling and emulsion stability are also provided. The methods for producing the gelatinous compositions and emulsified compositions comprise dissolving a phospholipid and a cyclic lipopeptide biosurfactant in a polyol solvent or a mixed solvent of water and a polyol to obtain a surfactant solution, and mixing an oily ingredient with the surfactant solution to obtain a gelatinous composition, wherein a mass ratio of the cyclic lipopeptide biosurfactant to the phospholipid in the gelatinous composition is 0.25 or more.

Provided is a method for preparing a compound represented by Formula I or a salt thereof, wherein Coleophoma empetri F-11899 (FERM BP2635) and / or a mutant strain thereof is cultured in a medium containing amino acid or a salt thereof, an insoluble organic source, and a sugar alcohol, to produce the compound of Formula I or a salt thereof.

The invention relates to the field of biology, in particular to a bacillus cereus Scu1 strain and application thereof. The strain is preserved in china center for type culture collection; the addressis Wuhan University, Wuhan, China; the post code is 430072; the preservation date is 18 December 2020; the preservation number is CCTCC NO: M2020915; and the taxonomy name is Bacillus cereus. The strain has an obvious inhibition effect on magnaporthe grisea, and the strain can generate an antibacterial substance cyclic lipopeptide surfactin.

The invention discloses a biosynthetic gene cluster of cyclic lipopeptide compounds, a method for activating the biosynthetic gene cluster and application thereof. Nucleotide sequences of the biosynthetic gene cluster of the cyclic lipopeptide compounds Totopotensamides are shown as 2972nd-101763rd bit nucleotide sequences in SEQ ID NO.1. The biosynthetic gene cluster, the method and the application have the advantages that all gene and protein information related to the biosynthetic gene cluster of the cyclic lipopeptide compounds Totopotensamides can assist people to understand biosynthesismechanisms of the cyclic lipopeptide compounds Totopotensamides, and materials and knowledge can be increased for further genetic modification; bases can be provided for activating expression of silent gene clusters by means of controlling regulatory genes, natural product biosynthetic gene cluster banks can be enriched, and entity can be provided for discovering lead compounds of medicines.

A process for purifying cyclic lipopeptide compounds or salts thereof comprising the steps of: (1) charging a crude compound of Formula I onto a macroporous adsorption resin; (2) washing the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as a washing liquid; and (3) eluting the compound of Formula I from the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as an eluent. The purification method has the advantages of using a small amount of organic solvents, using no silica gel, and causing little damage to the environment; the purity of the collected compound of formula I is also improved as compared with the methods previously disclosed.