17 results about "Magnaporthe grisea" patented technology

The invention discloses application of an OsGIT2 gene in rice blast resistance of rice. The applicant constructs a CRISPR carrier of the gene and transforms a japonica rice variety Kitaake of rice to obtain a transgenic line material, and the resistance of the transgenic line material to magnaporthe oryzae is obviously higher than that of a wild type material. The mutant rice is obtained by an applicant through a CRISPR-Cas9 technology, the mutant shows resistance to rice blast, and the technical scheme of the invention is of great significance to creation of broad-spectrum disease-resistant high-yield excellent new germplasm.

The invention belongs to the technical field of pesticide chemistry, and relates to a quinoline derivative containing a piperazine structure and a preparation method and application thereof. The invention provides a quinoline derivative containing a piperazine structure, and the quinoline derivative has broad-spectrum inhibitory activity on phytopathogens such as plant pathogenic fungi, especially has excellent inhibitory activity on valsa mali, sclerotinia sclerotiorum, botrytis cinerea, gaeumannomyces graminis and magnaporthe oryzae. The bactericide is expected to be developed into a novel green bactericide which is efficient, safe, economical and environment-friendly.

The application discloses a rice OsNAR1 application of the gene or the coded protein in regulating rice resistance to Magnaporthe oryzae, and relates to the technical field of rice Magnaporthe oryzae prevention and treatment. OsNAR1 The CDS nucleotide sequence of the gene is shown as SEQ ID No. 2. OsNAR1 The application finds that the rice gene OsNAR1 plays an important role in the process of rice resistance to Magnaporthe oryzae, the number of leaf spots on the gene deletion mutant is less, the gene can be used for screening rice strains resistant to Magnaporthe oryzae, the expression amount of a basic resistance marker gene in the gene deletion mutant is significantly up-regulated, and it is indicated that OsNAR1 negatively regulates the expression of the basic resistance gene and can be used for preventing and treating Magnaporthe oryzae.

The application discloses a method for cloning an avirulence gene from a small chromosome of Magnaporthe grisea, which comprises the following steps: (1) screening a strain carrying an avirulence gene corresponding to an anti-rice blast gene X; (2) performing ultraviolet mutagenesis on the screened strain; (3) spraying the strain after ultraviolet mutagenesis on a rice variety carrying the anti-rice blast gene X, and collecting the avirulence gene mutant strain; (4) performing mixed pool sequencing on the avirulence gene mutant strain obtained in the step (3); and (5) locating the avirulence gene through association analysis. The application does not depend on the fertility of the strain, sexual recombination and the genomic sequence polymorphism among different strains, and the target gene can be located through the association analysis by obtaining the avirulence gene mutant.

The invention provides application of a compound HLF1-11 in preparation of a medicine for preventing and controlling magnaporthe oryzae and a medicine for inhibiting spore germination of the magnaporthe oryzae, and further provides a pesticide and a method for preventing and controlling the magnaporthe oryzae. The compound HLF1-11 has an obvious inhibiting effect on development of magnaporthe oryzae appressorium, can effectively inhibit pathogenicity of magnaporthe oryzae spores and can prevent and treat magnaporthe oryzae, so that a foundation is laid for research and development of related prevention and control drugs.

The application discloses application of micafungin in preparation of a medicine for inhibiting fungi, and application of combination of micafungin and trifloxystrobin in preparation of the medicine for inhibiting fungi, especially for inhibiting Magnaporthe grisea. It is found by the application that micafungin has an inhibiting effect on Magnaporthe grisea. The combination of micafungin and trifloxystrobin has a synergistic effect, can overcome the drug resistance of Magnaporthe grisea with trifloxystrobin resistance, and improve the antibacterial effect on Magnaporthe grisea. It is found by the application that micafungin has a broad-spectrum antifungal effect, and has an inhibiting effect on other fungi such as Botrytis cinerea, Vibrio cholerae and Aspergillus niger in addition to Magnaporthe grisea.

The invention discloses application of induced culture of streptomyces bikini in improving resistance to magnaporthe oryzae, and belongs to the technical field of microbial control of plant diseases. The invention relates to application of GBL induced culture of streptomyces bikini to improvement of the magnaporthe oryzae resistance of the streptomyces bikini. The streptomyces bikini induced and cultured by the magnaporthe oryzae is applied to improvement of magnaporthe oryzae resistance. The method has an obvious enhancement phenomenon when being applied to inducing the antibacterial effect of the streptomyces bikini HD-087, and the GBL can be used for improving the yield of antibiotics in the streptomyces and improving the antibacterial capacity of the HD-087 in production. The oil discharge activity of the lipopeptide can be enhanced through treatment of the inducer, the lipopeptide has oil displacement capacity and shows certain potential in the aspect of grease degradation or removal, and a theoretical basis is provided for application of the lipopeptide in the fields of environmental restoration and the like. According to the application, the antibacterial effect of the HD-087 is improved, and meanwhile, the effect of repairing the environment is achieved.

The invention discloses an application of any compound of an Oxoapophine alkaloid derivative in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative shows certain inhibitory activity on two plant pathogenic bacteria such as rice bacterial blight and citrus canker pathogen, and five plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, magnaporthe grisea and wheat stem rot; the compound has a good inhibition effect on citrus canker pathogen and sclerotinia sclerotiorum. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel agricultural antibacterial agent.

The invention discloses an N-pyrazole-4-yl benzamide derivative of a diphenyl ether fragment containing a halogen-mixed substituent group and a preparation method and application thereof, and relates to the field of pesticide bactericides, and the structural general formula is as shown in formula (I). A bacteriostatic activity test is carried out on plant pathogenic fungi by using the pyrazole amide derivative disclosed by the invention, and a test result shows that the compounds have a good bacteriostatic effect on the plant pathogenic fungi such as pyricularia oryzae, fusarium graminearum, cucumber target leaf spot bacteria, rhizoctonia solani and botrytis cinerea; the invention provides a direction for further development of a novel succinate dehydrogenase inhibitor.

The application belongs to the technical field of RNA biological control, and particularly relates to application of dsRNA targeting actin coding genes. The application provides application of dsRNA targeting actin coding genes in prevention and treatment of crop anthracnose, wherein the nucleotide sequence of the dsRNA is shown as SEQ ID No. 2, the dsRNA is transcribed from a target gene segment of a Magnaporthe grisea CaACTIN gene, and the nucleotide sequence of the target gene segment of the Magnaporthe grisea CaACTIN gene is shown as SEQ ID No. 1. By loading the above dsRNA on a nano material layered double hydroxide, the pathogenicity of mango anthracnose and banana anthracnose can be obviously reduced, and the application prospect of the dsRNA in high-efficiency and targeted prevention and treatment of crop anthracnose is high.

The invention discloses an application of genes OsEDS1 and OsPAD4 in improving the disease resistance of plants, and the genes OsEDS1 and OsPAD4 can enhance the disease resistance of the plants to magnaporthe oryzae infection and are used for cultivating new varieties of disease-resistant plants.

The application discloses application of a compound BTD-81 in preparation of a medicine for preventing and treating Pyricularia oryzae, and belongs to the field of plant fungal disease prevention and treatment. The compound BTD-81 has a remarkable effect in preventing and treating rice blast or / and other pathogenic fungi, can effectively inhibit spore pathogenicity of the Pyricularia oryzae in a concentration range of 10-50 ppm, and especially, the compound BTD-81 at 50 ppm can completely prevent and treat the rice blast. The compound BTD-81 can be used as a protective agent, has a better effect when used at an initial stage of the rice blast infection, and under the condition of spraying the compound, the growth state of the rice is good, and there is no obvious adverse effect, indicating that the compound is safe to the rice, and has a practical significance for preventing the rice blast disease.

The invention discloses application of an FAH1 gene in improving resistance of rice to rice blast. The nucleotide sequence of the FAH1 gene is as shown in SEQ ID NO.1, and the amino acid sequence coded by the FAH1 gene is as shown in SEQ ID NO.2. The FAH1 gene is knocked out in a rice plant, and the resistance of rice to pyricularia grisea can be remarkably improved. The invention provides a basis for the key molecular genetic mechanism of rice blast resistance, and has important guiding significance for cultivating resistant rice varieties and improving the rice quality.

The application discloses application of a compound MSP-10 in preparation of a medicine for preventing and treating Magnaporthe oryzae, belongs to the field of plant fungal disease prevention, and the compound MSP-10 can prevent and treat Magnaporthe oryzae ( Magnaporthe oryzae ) or / and other pathogenic fungi. The compound MSP-10 of the application has certain prevention and treatment effect when being mixed with spore liquid of Magnaporthe oryzae and sprayed at 0 h, indicating that the compound MSP-10 can be used as a protective agent at the initial stage of Magnaporthe oryzae infection and has better effect; moreover, the rice sprayed with the compound has a good growth trend and has no obvious adverse effect, indicating that the compound is safe to the rice and has practical significance for preventing the Magnaporthe oryzae disease.

The application discloses application of a rice OsSIT2 gene in rice blast resistance, and discloses a rice OsSIT2 gene CDS nucleotide sequence shown in SEQ ID NO.1. The application finds that the rice OsSIT2 gene plays an important role in the process of resisting rice blast of rice. The deletion of the rice OsSIT2 gene reduces the resistance of rice to a physiological race RB22 of Magnaporthe grisea, so the rice OsSIT2 gene has important significance for cultivating rice plants resistant to rice blast, and is suitable for popularization and application.

The invention belongs to the technical field of biological prevention and control of forestry diseases, and discloses a microorganism for inhibiting chaetosporum oleolatum, which is Erwinia separated from leaf rhizosphere of pinus tabulaeformis, is preserved in China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC NO.36570. The invention further discloses a preparation method of the microorganism for inhibiting chaetosporum oleolaeformis. The screened microorganism for inhibiting the chamomile oil is high in environmental adaptability, excellent in antibacterial effect on the chamomile oil and high in host adaptability, does not cause harm to non-target organisms in Chinese pines and forest ecosystems, and can also avoid residual pollution of traditional chemical agents. On the basis of the microorganism which is separated, purified and cultured, the microorganism can be further prepared into fungicide such as wettable powder and a suspending agent, can be used for field prevention and treatment of pinus tabulaeformis needle drop disease, and can also be used as a core strain of a composite biocontrol fungicide, so that the application scene is expanded.

The application discloses a method for constructing a large fragment deletion mutant library of Magnaporthe oryzae, and belongs to the field of agricultural biotechnology.The method comprises the step of adding DEB to the protoplast of the Magnaporthe oryzae for culture and inducing the production of conidia.The application utilizes the chemical mutagen DEB to obtain the Magnaporthe oryzae mutant at a high frequency on a whole genome scale, and provides a new technical path for the functional genomics research of the Magnaporthe oryzae.The method provided by the application is helpful to systematically analyze the pathogenic mechanism, the infection process and the environmental adaptation regulation network of the Magnaporthe oryzae, and provides support for mining new disease-resistant targets and disease-resistant mechanisms.The method has the advantages of high efficiency, low cost and short cycle, and has wide application potential in the genetic research of plant pathogenic fungi.