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127 results about "Magnaporthe grisea" patented technology

Magnaporthe grisea, also known as rice blast fungus, rice rotten neck, rice seedling blight, blast of rice, oval leaf spot of graminea, pitting disease, ryegrass blast, and Johnson spot, is a plant-pathogenic fungus that causes a serious disease affecting rice. It is now known that M. grisea consists of a cryptic species complex containing at least two biological species that have clear genetic differences and do not interbreed. Complex members isolated from Digitaria have been more narrowly defined as M. grisea. The remaining members of the complex isolated from rice and a variety of other hosts have been renamed Magnaporthe oryzae. Confusion on which of these two names to use for the rice blast pathogen remains, as both are now used by different authors.

Plant source pesticide for inhibiting rice blast and application thereof in aspect for inhibiting rice blast

The present invention discloses a plant pesticide which restrains the rice blast and the application of the plant pesticide for restraining rice blast. The plant pesticide is a low-toxicity and high-efficient novel biological product which is developed by using the plant of Chinese scholar tree as raw material. The pesticide is applied with a mode of foliage spray and can be applied to the agricultural field. The plant pesticide for restraining the rice blast according to the invention is obtained through the following steps: using the Chinese scholar tree as raw material, putting the raw material into an extraction kettle after breaking the tissue of raw material, adding the organic solvent with the weight of 2-3 times of the weight of raw material, immersing and extracting for 12-24 hours in the condition of room temperature, extracting for 2-3 times; condensing the extracted liquid to solid-state concentrate; dissolving the solid-state concentrate into the organic solvent with the weight of 0.5-1.5 times of the weight of solid-state concentrate, adding the emulsifying agent with the weight of 0.1-0.15 times of the weight of solid-state concentrate, sufficiently mixing, adding the water with the weight of 20-40 times of the weight of solid-state concentrate, and the plant pesticide aqueous emulsion for restraining rice blast is prepared. The plant pesticide aqueous emulsion for restraining rice blast can effectively restrain the magnaporthe grisea, increases the quality of arable farming and strengthens the capability of crop for resisting disasters and pests.
Owner:NANJING UNIV OF INFORMATION SCI & TECH

Method for detecting magnaporthe grisea genes for enhancing magnaporthe grisea strain pathogenicity

InactiveCN105177145AIncrease infectivityOvercoming the disadvantages of understanding the infectivity of germsMicrobiological testing/measurementMicroorganism based processesBiotechnologyReal-Time PCRs
The invention discloses a method for detecting magnaporthe grisea genes for enhancing magnaporthe grisea strain pathogenicity, and relates to the field of plant protection and biological technology. The method comprises the steps that a reaction of resistance or susceptibility and a real-time RT-PCR are inspected for analyzing defense related gene expression of rice early stage response and a qPCR is adopted for detecting the relative fungus growth amount of disease spots; a prokaryotic expression product of the genes is sprayed to leaves for 24 h on the basis of the concentration being 1.0 microgram/ml-6.0 microgram/ml, and then strong/weak pathogenic strains are inoculated; the reaction of resistance or susceptibility and the real-time RT-PCR are inspected for analyzing rice defense related gene expression; overexpression strains of the genes are utilized for being inoculated on rice blades in an in-vitro mode, and the reaction of resistance or susceptibility and the real-time RT-PCR are inspected for analyzing the rice defense related gene expression and the qPCR is inspected for analyzing relative fungus growth amount on disease spots. The method can comprehensively and accurately clear pathogen effect protein genes and improve the pathogen infective ability, and the accurate and comprehensive method is provided for determining the pathogen effect protein for improving the pathogen infective ability in the future.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

Method for separating and screening out antagonistic bacterium from lycoris aurea bulb

The invention relates to a method for separating and screening out an antagonistic endophytic bacterium from a lycoris aurea bulb. According to the method, by using an endophyte separation method, an endophytic bacterium is obtained by separating the endophytic bacterium from the lycoris aurea bulb; an improved confrontation culture method is adopted, test magnaporthe grisea is dripped in the center of a flat plate, and is cultured for 2-3 days; four crosswise corners around the magnaporthe grisea are inoculated with four bacterial strains respectively, and culture is carried out for 5-6 days; through observation, a bacterium having a bacteriostatic effect is the antagonistic bacterium obtained through screening. The antagonistic bacterium having an obvious inhibiting effect on magnaporthe grisea of rice is obtained; an antimicrobial spectrum experiment proves that the antagonistic bacterium also has an inhibiting effect on fusarium graminearum, aspergillus fumigatus and candida albicans. By adopting the method disclosed by the invention, the screening efficiency can be remarkably improved, and the antagonistic bacterium obtained by separation can be used as a biocontrol bacterium for being prepared into a microbial preparation, thus providing a basis for biological control over diseases.
Owner:INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI

A kind of camel velvet transfer protein and its preparation method and application

The invention discloses a peganum harmala lipid transfer protein (PHP) and relates to the preparation and use of new protein with antifungal, anticancer and antivirus activities. The method uses seeds of a peganum harmala plant as raw materials and comprises the following steps: preparing a coarse product, performing cationic exchange chromatographic separation and purifying by using a molecular sieve; and gradually separating and collecting a purified component having antibacterial activity, dialyzing, desalting, freeze-drying and thus, obtaining purified PHP. In the invention, a filter paper disc agar diffusion method is adopted, and researches prove the protein has growth inhibiting effect on five plant pathogenic fungi, namely alternaria alternate, penicillium degitatum, rhizopus stuolonifer, magnaporthe grisea and penicillium italicum. An 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method proves that the protein has inhibiting effect on proliferation of human esophageal carcinoma cell Eca109, human cervical cancer cells HeLa, human stomach cancer cells MGC-7, mouse melanoma cells B16 and the like. The protein can inhibit the activity of human immunodeficiency virus 1 (HIV-1) reverse transcriptase. The protein can be used for developing medicines for resisting agricultural pathogenic fungi, tumors and viruses.
Owner:XINJIANG UNIVERSITY

Preparation method of fertilizer for preventing and controlling rice blast

The invention discloses a preparation method of a fertilizer for preventing and controlling rice blast, and belongs to the field of fertilizers. According to the preparation method provided by the invention, brassinolide is compounded with diethyl aminoethyl hexanoate, and the main effects are as follows that on one hand, the carbon and nitrogen metabolism of plants are promoted, so that the vigorand the development of rice are improved; on the other hand, the crop yield is improved, and the crop quality is improved. According to the preparation method, a penetration enhancer is penetrated into the roots of the rice, the enzyme activation and the photosynthesis are promoted through the brassinolide and the diethyl aminoethyl hexanoate; then the activity of separately cultivated strains isgreatly increased under the action of the enzyme activation, so that the capability of resisting the invasion of magnaporthe grisea is promoted; and on the other hand, the active substances of the stems and leaves of kiwifruits are added, the propagation and the growth of the magnaporthe grisea in rice are inhibited, and the rice blast is controlled. The preparation method solves the problem thatsterilization agents are adopted to prevent and control the rice blast in the prior art, a large amount of manpower and material resources are wasted, and unnecessary pollution is caused to the environment, and unnecessary pesticide residue is generated in the rice.
Owner:芮志行
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