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3 results about "Magnaporthe grisea" patented technology
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Magnaporthe grisea, also known as rice blast fungus, rice rotten neck, rice seedling blight, blast of rice, oval leaf spot of graminea, pitting disease, ryegrass blast, and Johnson spot, is a plant-pathogenic fungus that causes a serious disease affecting rice. It is now known that M. grisea consists of a cryptic species complex containing at least two biological species that have clear genetic differences and do not interbreed. Complex members isolated from Digitaria have been more narrowly defined as M. grisea. The remaining members of the complex isolated from rice and a variety of other hosts have been renamed Magnaporthe oryzae. Confusion on which of these two names to use for the rice blast pathogen remains, as both are now used by different authors.
A method for cloning an avirulence gene from a small chromosome of rice blast fungus
ActiveCN121555524Bfast cloneMicrobiological testing/measurementMutant preparationMagnaporthe griseaGene Mutant
The application discloses a method for cloning an avirulence gene from a small chromosome of Magnaporthe grisea, which comprises the following steps: (1) screening a strain carrying an avirulence gene corresponding to an anti-rice blast gene X; (2) performing ultraviolet mutagenesis on the screened strain; (3) spraying the strain after ultraviolet mutagenesis on a rice variety carrying the anti-rice blast gene X, and collecting the avirulence gene mutant strain; (4) performing mixed pool sequencing on the avirulence gene mutant strain obtained in the step (3); and (5) locating the avirulence gene through association analysis. The application does not depend on the fertility of the strain, sexual recombination and the genomic sequence polymorphism among different strains, and the target gene can be located through the association analysis by obtaining the avirulence gene mutant.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
Application of rice OsSIT2 gene in regulating rice resistance to rice blast
The application discloses application of a rice OsSIT2 gene in rice blast resistance, and discloses a rice OsSIT2 gene CDS nucleotide sequence shown in SEQ ID NO.1. The application finds that the rice OsSIT2 gene plays an important role in the process of resisting rice blast of rice. The deletion of the rice OsSIT2 gene reduces the resistance of rice to a physiological race RB22 of Magnaporthe grisea, so the rice OsSIT2 gene has important significance for cultivating rice plants resistant to rice blast, and is suitable for popularization and application.
Owner:CHINA AGRI UNIV
A method for constructing a library of large fragment deletion mutants of magnaporthe grisea
The application discloses a method for constructing a large fragment deletion mutant library of Magnaporthe oryzae, and belongs to the field of agricultural biotechnology.The method comprises the step of adding DEB to the protoplast of the Magnaporthe oryzae for culture and inducing the production of conidia.The application utilizes the chemical mutagen DEB to obtain the Magnaporthe oryzae mutant at a high frequency on a whole genome scale, and provides a new technical path for the functional genomics research of the Magnaporthe oryzae.The method provided by the application is helpful to systematically analyze the pathogenic mechanism, the infection process and the environmental adaptation regulation network of the Magnaporthe oryzae, and provides support for mining new disease-resistant targets and disease-resistant mechanisms.The method has the advantages of high efficiency, low cost and short cycle, and has wide application potential in the genetic research of plant pathogenic fungi.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI