Pseudomonas aeruginosa JT86 and application of pseudomonas aeruginosa JT86 in prevention and treatment of sclerotiniose

A Pseudomonas aeruginosa, JT86 technology, applied in the application, bacteria, fungicides and other directions, can solve problems such as disease control difficulties, natural environment damage, etc., to achieve extensive development and application prospects, reduce use, and reduce pesticide residues.

Active Publication Date: 2020-08-25
海峡两岸农业科技股份有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the perennial use of chemical pesticides has caused great damage t

Method used

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  • Pseudomonas aeruginosa JT86 and application of pseudomonas aeruginosa JT86 in prevention and treatment of sclerotiniose
  • Pseudomonas aeruginosa JT86 and application of pseudomonas aeruginosa JT86 in prevention and treatment of sclerotiniose
  • Pseudomonas aeruginosa JT86 and application of pseudomonas aeruginosa JT86 in prevention and treatment of sclerotiniose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Isolation and screening of Pseudomonas aeruginosa (Pseudomonas aeruginosa) JT86

[0039]1. Sample collection:

[0040] Soil samples were collected from the paddy fields of Yuejin North Farm, South China Agricultural University, Tianhe District, Guangzhou City, Guangdong Province, and placed in sterile centrifuge tubes and brought back to the laboratory for strain isolation.

[0041] 2. Separation of biocontrol bacteria:

[0042] After mixing the soil sample evenly, take 5g of the soil sample and place it in a 50mL sterile centrifuge tube, add sterile water to 50mL, shake vigorously to suspend the soil sample, and mark it as 1×10 -1 . Then pipette 1 mL of 1×10 -1 the soil sample suspension into a new centrifuge tube filled with 9mL sterile water, and successively diluted to 1×10 -2 , 1×10 -3 series concentration soil solution. Draw 0.1mL of 1×10 -2 , 1×10 -3 Put the soil solution on a solid LB plate (yeast extract 5g, tryptone 10g, NaCl 5g, water 1000mL...

Embodiment 2

[0050] Example 2 Identification of Pseudomonas aeruginosa (Pseudomonas aeruginosa) JT86

[0051] 1. Morphological identification

[0052] (1) Experimental method

[0053] The JT86 strain screened in Example 1 was cultured on LB medium, the morphological characteristics were observed, and physiological and biochemical identification was carried out.

[0054] (2) Experimental results

[0055] The morphological characteristics of JT86 strain are as follows: figure 1 As shown, it can be seen that the colony shape of the strain is round, light yellow, with smooth edges, raised center, smooth surface, dull, translucent, Gram staining negative, and the electron microscope shows that the bacteria are short oval Rod-shaped, single cell, cell size 1.8×3.0μm.

[0056] In addition, the physiological and biochemical identification results found that the physiological and biochemical tests of the JT86 strain were positive in contact enzyme reaction, oxidase reaction, motility measuremen...

Embodiment 3

[0067] Example 3 Inhibition experiment of Pseudomonas aeruginosa (Pseudomonas aeruginosa) JT86 on sclerotia germination

[0068] 1. Experimental method

[0069] Take the Pseudomonas aeruginosa JT86 stored in glycerol at -80°C, activate it on LB solid medium at 30°C for 48 hours, dip the single colony of the activated Pseudomonas aeruginosa JT86 into 100mL NA liquid medium, and set it at 28°C at 200rpm Shake the bacteria for 48 hours. Afterwards, according to the 10% inoculum size, the secondary culture was carried out, that is, 10% of the bacterial liquid was taken in 100 mL of NA liquid medium, and the bacterial culture was shaken at 200 rpm at 28° C. for 48 hours to obtain the fermentation broth of Pseudomonas aeruginosa JT86.

[0070] Pick 120 sclerotias of rice sheath blight bacteria, peanut white sclerotia and rape sclerotinia of the same size, set up the control group and treatment group for 3 repetitions, and carry out surface disinfection on the sclerotia, that is, us...

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Abstract

The invention discloses pseudomonas aeruginosa JT86 and an application of the pseudomonas aeruginosa JT86 in prevention and treatment of sclerotiniose. The pseudomonas aeruginosa JT86 is screened fromsoil, and the strain is collected in the Guangdong Microbiological Culture Collection Center on September 30, 2019, and has a collection number of GDMCC NO:60799. The strain and a volatile matter thereof can completely inhibit sclerotium germination of rhizoctonia solani, sclerotium rolfsii and sclerotinia sclerotiorum and inhibit growth of sclerotium hyphae, so that the sclerotium hyphae have flakes and wrinkles; the strain also has a good inhibition effect on the rhizoctonia solani, the sclerotium rolfsii, the sclerotinia sclerotiorum, magnaporthe grisea, fusarium oxysporum f. sp. cubense,brassica parachinensis anthracnose, colletotrichum gloeosporioides and colletotrichum capsici; and therefore, the strain can treat recalcitrant soil-borne pathogenic bacteria in soil in a targeted manner, effectively inhibits the sclerotiniose, and has a good application prospect in preparation of a soil treatment agent for preventing and treating soil-borne fungal diseases.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control. More specifically, it relates to a strain of Pseudomonas aeruginosa JT86 and its application in the prevention and treatment of Sclerotinia. Background technique [0002] Soil-borne diseases caused by plant pathogenic fungi are an important bottleneck restricting the sustainable development of agricultural production in my country. Compared with plant pathogens such as bacteria and nematodes, the soil physical and chemical environment is generally not conducive to the growth of plant pathogenic fungi, so plant pathogenic fungi usually exist in the soil as some propagules (such as sclerotia, thick-walled conidia), or saprophyte in the soil. Plant roots and crop residues. Because plants and pathogenic fungi form a highly specialized system of mutual selection and co-evolution during the interaction process, a wide variety of soil-borne pathogenic fungi have different spec...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/27A01P3/00C12R1/385
CPCC12N1/20A01N63/00C12R2001/385C12N1/205
Inventor 舒灿伟李月周而勋杨媚古开平林镇彬
Owner 海峡两岸农业科技股份有限公司
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