741 results about "Sclerotium" patented technology

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

The invention discloses a morel indoor cultivation method and a greenhouse thereof. The method includes the following steps: (1) cultivation material is prepared; (2) filling is carried out, namely equivoluminal cultivation material and soil are filled in a disinfected bag or tank; (3) disinfection is carried out; (4) inoculation is carried out, namely agar containing morel is placed on the soil layer in the step (2), and sclerotium is inoculated; (5) sclerotium growth is carried out in dark place, and temperature and relative air humidity are maintained; (6) culture medium is prepared, namely the sclerotium is uniformly intercalated into two soil layers with the thickness of 1-3cm, and indoor temperature and relative air humidity are maintained; (7) watering is carried out on the culture medium until the culture medium is completely permeated by water; (8) uniform ventilation is carried out, (9) morel is matured, namely the humidity of the culture medium is maintained, and meanwhile the indoor temperature and relative air humidity are maintained until the morel is matured. By adopting the method, indoor cultivation of morel having high nutrition value can be realized, mass production can be carried out, and yield is high.

The invention discloses a cut-log cultivation method of polyporusrhinocerus cooke. The method comprises manufacturing of a polyporusrhinocerus cooke parent seed, an original seed and a cut-log cultivation seed, land selecting and preparing, cut-log cultivation seed burying and field managing and collecting. The method overcomes the detects in the prior art, wild growing environment of the polyporusrhinocerus cooke is simulated, the polyporusrhinocerus cooke is cultivated by adopting the cut-log cultivation method, the requirements of nutrition of polyporusrhinocerus cooke sclerotium are met, and therefore superior sclerotium is formed.

The invention relates to bacillus subtilis Pc3 and a use of bacillus subtilis Pc3 in preparation of a fermentation supernatant for preventing and controlling plant pathogenic fungi, and relates to bacilli. The bacillus subtilis Pc3 has a preservation number of CCTCC NO: M2013470. The bacillus subtilis Pc3 can prevent and control plant pathogenic fungi such as gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani and fusaria. A rape sclerotinia sclerotiorum prevention and control pot experiment proves that the bacillus subtilis Pc3 has good biological-prevention and control effects and has prevention and control efficiency of 95.6%. The fermentation supernatant of the bacillus subtilis Pc3 can be prepared by simple processes and has a wide fungus inhibition spectrum and good biological prevention effects.

The present invention provides a conversion bag for promoting formation of common morel sclerotium. The conversion bag is prepared by the method comprising the following steps of: (1) obtaining dry wheat, soaking the dry wheat in whitewash having concentration of 1% for 48 hours, filtering, abandoning solution, and obtaining the processed wheat; and (2) adding gypsum having the weight accounting for 1% of the weight of the dry wheat into the processed wheat, mixing, bagging, sterilizing, and obtaining the conversion bag. The present invention also provides the purpose of the conversion bag in common morel cultivation. The present invention further provides a common morel cultivation method. By utilization of the common morel cultivation conversion bag and the cultivation method, yield of common morel artificial cultivation can be largely increased, and the common morel cultivation conversion bag and the cultivation method are low in cost and simple in operation, and have good application prospect.

A construction method for antagonistic Trichoderma-Brassica napus joint adsorption of heavy metals cadmium and zinc in farmland soil in the field of metal adsorption technology, by inoculating bacterial agents prepared by adsorbing heavy genus antagonistic Trichoderma strains to bacteria containing Cd or Zn and Sclerotinia sclerotiorum The soil of the wheat grain culture is further used to cultivate Brassica napus and obtain the Trichoderma-rape symbiont with high efficient adsorption of heavy metals. The invention realizes the dual purposes of bioremediation of heavy metal pollution in farmland soil and treatment of soil-borne diseases, has good repair effect, low cost, is easy to manage and operate, and does not produce secondary pollution, so it has high universal use value in the field.

The invention provides a special organic-inorganic composite biological pesticide-fertilizer for tea. The special organic-inorganic composite biological pesticide-fertilizer for tea is characterized by comprising the following raw materials in parts by weight: 70-90 parts of urban domestic garbage, 10-15 parts of guano, 5-8 parts of bone meal, 4-6 parts of gelatin, 20-25 parts of tree ash, 20-25 parts of modified diatomite, 5-10 parts of monoammonium phosphate, 12-15 parts of urea, 12-15 parts of mineral slag, 5-10 parts of potassium chloride, 3-5 parts of paulownia sawdust, 10-12 parts of animal hair, 3-5 parts of sclerotium mylittae, 3-5 parts of betelnut, 3-5 parts of herba artemisiae scopariae, 5-8 parts of radix sophorae flavescentis, 3-5 parts of radix scutellariae, 10-15 parts of sodium fulvate, 3-5 parts of peat, 0.08-0.12 part of ammonium molybdate, 1.5-2.5 parts of ferrous sulfate, 0.7-1.0 part of copper sulfate, 0.8-1.2 parts of borax and 0.1-0.2 part of EM (effective microorganism) strain. The pesticide-fertilizer disclosed by the invention has the advantages of scientific ratio of nitrogen, phosphorus and potassium, easiness in absorption by crops, obvious yield-increasing benefit and the like. By applying the pesticide-fertilizer, the nutrient absorption of trees can be balanced, the tea quality can be effectively improved and the taste of the tea can become thick.

The invention relates to a Sclerotium rolfssii scleroglucan online fermentation extraction method, which comprises the following steps: (1) culturing a strain; (2) fermenting the Sclerotium rolfssii by using a fermentation tank; and (3) extracting polysaccharide liquid on line by ceramic membrane separation. The invention also relates to a Sclerotium rolfssii scleroglucan online fermentation extraction system, which comprises the fermentation tank, a feeding system, an oxygen supply system, a steam system, a control system and a ceramic membrane separation system. In the Sclerotium rolfssii scleroglucan online fermentation extraction method and the Sclerotium rolfssii scleroglucan online fermentation extraction system, a Sclerotium rolfssii glucan product is continuously extracted from fermentation liquor by ceramic membrane separation in a fermentation process, the Sclerotium rolfssii glucan does not accumulated in the fermentation liquor, and thus, the inhibition of the synthesis ofthe Sclerotium rolfssii glucan product due to problems of increases in viscosity of fermentation liquor, reduction in dissolved oxygen, reduction in pH value and the like. Meanwhile, post treatment is not required, the yield is high, the production process is simplified, the equipment cost is low, and the operation cost is low.

The invention discloses a process for manufacturing high-quality morchella strains. The process includes selecting acquisition time of morchella; screening high-quality strains; cultivating mycelia; manufacturing and cultivating mother strains; manufacturing and cultivating cultivated strains. The morchella is acquired in the seventh-tenth days when first or second crops of morchella are fruited in February or March of the second year of morchella planting under the conditions that morchella individuals are longer than or equal to 10cm and are shorter than or equal to 15cm, pilei of the morchella individuals are plump, ridges and pits on the pilei are completely spread and stipites of the morchella individuals are milky; the high-quality strains are ejected and screened step by step according to forms and colors of the pilei, colors and forms of the stipites, colors and quantities of spores and sclerotium growing ability of the strains. The process has the advantages that the yield of fresh morchella which grows from the high-quality morchella strains manufactured by the aid of the process is stable for three continuous years and is stabilized at about 80kg, the fruiting time is stable, the growing speeds of the individuals are similar to one another, and the technical problem of instable yields in current morchella industrial development can be solved.

The invention belongs to the technical field of biological pesticide, in particular to screening and identification of a coniothyrium minitans strain with strong parasitism and saprogenicity on sclerotinia sclerotium and an application thereof in a microorganism agent. The coniothyrium minitans ZS-1SB strain is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number being CCTCC NO: M209114. The invention also discloses an open-type solid fermentation cultivation method of the microorganism agent and an application of the coniothyrium minitans ZS-1SB microbial inoculum in the prevention and treatment of sclerotinia of colza. The microorganism agent of the invention has excellent biological prevention effect on plant fungous diseases such as sclerotinia rot of colza and the like.

The invention relates to a rapid identification method for pathogenic fungi, which specially comprises the following steps: (1) culturing fungi on a solid culture medium; (2) taking fresh fungi, and extracting the DNA of the fungi by using a microwave method; (3) amplifying DNA fragments between ribosomal spacers of the fungi by using ITS1 and ITS4 primers through PCR (polymerase chain reaction); (4) after recovering the PCR fragments, carrying out sequence determination on the fragments; and (5) carrying out comparison on the obtained sequences by using the Blast of NCBI. According to the invention, the identification of unknown pathogenic fungi can be performed within a shorter time and under relatively simple experimental conditions, thereby facilitating the rapid diagnosis for diseases occurring in fields so as to guide the prevention and control of diseases. According to the method, through the identification on 21 kinds of pathogenic fungi on different crops, and the coincidence rate is 100% through sequence comparison. The method is not only applicable to the identification on fresh mycelia, but also is applicable to the identification on conidium and sclerotium of pathogenic fungi, and has the characteristics of rapidness and high efficiency.

The invention relates to a manual wild-imitation cultivation technology for pure strains of grifola. The manual wild-imitation cultivation technology comprises the steps of land selection and land preparation, cultivation, seeding methods, management after seeding, management in a coming year, management in a third year, excavation and drying processing. In a biological angle, wild sclerotium tubers are polluted in the natural environment, and purity of the pure strains is achieved. Besides, the pure strain only have fungus age of half a year, wild grifola fungus tubers have the longest fungus age of five years, and the reproduction rate of the pure strains is one time more than that of the wild grifola fungus tubers.

The invention discloses a sclerotium rolfsii solid fermentation culture medium and an application thereof. The solid fermentation culture medium comprises a solid substrate and water, wherein the solid substrate comprises wheat bran and agricultural wastes, the wheat bran accounts for 20%-60% of the total weight of the solid substrate, and the weight ratio of the water to the solid substrate is (20-45):100; and the agricultural wastes select rice husk, sweet sorghum straw, an organic fertilizer, a wheat bran substrate, vinegar residue, wine lees, garden dead twigs or sawdust. The sclerotium rolfsii solid fermentation culture medium prepared by the preparation method disclosed by the invention is low in cost, the preparation process is simple, and a herbicide prepared by adopting the solid fermentation culture medium can be preserved at room temperature.

The invention relates to a traditional Chinese medicine insecticide and a preparation method thereof; the traditional Chinese medicine insecticide comprises the herbal medicine of Chinese azalea fruit, flos daturae, arisaema, anisodus acutangulus, sclerotium mylittae, radix aconiti agrestis, bluestone, aconite, cinnabar, croton and euphorbia. The herbal medicine is prepared into the traditional Chinese medicine insecticide after being processed, decocted and filtered, various agricultural pests can be effectively killed, the killing rate is above 90 percent, and the pure traditional Chinese medicine insecticide is green and environmental-friendly, does not pollute the environment and crops, is convenient to prepare, is low in cost, and is easy to popularize and use.

The invention provides a manufacturing method for morchella mother culture. The method is characterized by comprising the following steps of: 1, boiling 1 to 3 parts of fresh insect-free populus bonatii smashed branches or populus bonatii leaves in 10 parts of water based on a mass ratio for 25 to 30 minutes, filtering the mixture, and taking juice to prepare populus bonatii leachate; 2, mixing the populus bonatii leachate and PDA culture solution in a mass ratio of 1: 1, adding 0.2 percent of Na3PO4.12H2O, 0.2 percent of KH2PO4, 0.05 percent of MgSO4 and 0.005 percent of VB1 based on the mass percent of the mixed liquor, uniformly stirring the mixture, and sterilizing the mixture at the temperature of 121 +/1 DEG C for 30 minutes to prepare a culture medium; and 3, cooling the culture medium, inoculating the morchella mother culture, culturing the inoculated culture medium at the constant temperature of 23 DEG C for 10 to 12 days, and culturing the culture medium in an incubator at the temperature of between 8 and 12 DEG C for 4 to 6 days. The manufacturing method has the advantages of strong hypha, strong twisting force, and easy formation of sclerotium on protospecies or cultivar.

The invention provides an efficient and non-toxic broad-spectrum insectofungicide as well as a preparation method and a use method thereof. The insectofungicide comprises the following components in parts by weight: 100 parts of water, 15-23 parts of alkaloids from tripterygium wilfordii, 1-5 parts of triptolide, 5-10 parts of omphalin, 3-8 parts of chamaejasmine, 8-20 parts of nicotine, 4-8 parts of capsaicine, 1-2 parts of suberosin and 1-3 parts of benzylpenicillin sodium, wherein the alkaloids from tripterygium wilfordii are extracted from roots, stems and leaves of tripterygium wilfordii; the triptolide is extracted from the root of tripterygium wilfordii; the omphalin is extracted from omphalialapidescens sclerotium; the chamaejasmine is extracted from stellera chamaejasme L.; the tobacco stalk cellulose is extracted from the branches and leaves of the tobaccos; the capsaicine is extracted from the capsicum fruits; and the suberosin contains sanshool and sanshoamide and is extracted from the pepper fruits. The insectofungicide is non-toxic and harmless to human and livestock, does not pollute the environment and the crops and has no toxic residues. The product has the effectof controlling the diseases and insect pests of the crops, fruit trees and vegetables.

The invention relates to the technical field of plant cultivation and discloses a planting method of rhizome corydalis. The planting method includes the steps of land selection, planting, field management, reasonable fertilization, disease control, pest prevention and control and harvesting. Before sowing rhizome corydalis seeds, the seeds are dressed with 40% of carbendazim, so that downy mildew of seedlings is decreased, and stalk break of the seedlings can be effectively prevented by spraying metalaxyl manganese zinc or aliette on the seedlings in later period; base fertilizers reasonably in recipe are applied during seedling, effectiveness can be achieved rapidly, the seedlings can direct absorb the base fertilizers, and nutritional requirements of the rhizome corydalis during growing can be met; the rhizome corydalis planted in the method is attractive in appearance, high in survival rate and yield, good in rootstock of the rhizome corydalis and good in drug property.

The invention relates to a method for identifying the resistance of sclerotinia by inoculating an in-vitro stalk of a plant. The method comprises the following steps of: cutting the stalk or branches of the plant down, and putting the stalk or the branches into closed environment in which temperature and humidity are constant to inoculate sclerotinia sclerotiorum; and recording the extension lengths of bacterial plaques to measure the strength of disease resistance of the material. Compared with the conventional method for identifying the resistance of the stalk with sclerotinia, the method has the advantages that the method is performed under the condition of controllable environment, the pathogenetic condition is consistent, and inoculating identification is stable; the method is simple, short in period and high in inoculating efficiency; and inoculated parts are flexible, and a single plant can be identified repeatedly, so the method is suitable for the large-scale inoculating identification of the material.