23 results about "Sexing" patented technology

This invention discloses an automatic weighing, sex identification, and information-based sorting system and method for crabs based on embedded control and machine vision. The system acquires weight information, ventral biotexture features, dorsal biotexture features, and micro-amplitude response features through a pre-processing acquisition link to establish an initial sorting judgment. Then, a verification label is generated and affixed through a verification label generation and affixing link. Subsequently, the verification label information is read and verification features are acquired again through a label reading and consistency verification link. Based on the consistency verification result, a decision is made on whether to proceed with formal sorting or handle the anomaly. This scheme can reduce the risk of missorting caused by mismatch between labels and actual crabs, and improve sorting accuracy and information label reliability.

This invention discloses a method for large-scale breeding of all-male salmon and trout, belonging to the field of fish sex regulation breeding technology. The method includes: collecting fertilized eggs from different families of the same species of salmon and trout, artificially incubating them to obtain fry, and inducing sex reversal in the fry by using 17β-estradiol when they begin feeding; subsequently, raising the fry to sexual maturity, selecting individuals with a female phenotype, and then using sdY as a sex identification gene, selecting pseudo-females with genotype XY by quantitative real-time PCR; pairing the sexually mature pseudo-females with genotype XY with normal males with genotype XY, and obtaining super-males with genotype YY through artificial insemination; pairing the sexually mature super-males with females with genotype XX, and producing all-male salmon and trout with genotype XY on a large scale through artificial insemination. This invention overcomes the problem that existing technologies cannot achieve large-scale breeding of all-male salmon and trout.

This invention discloses a non-destructive method for sex detection of chicken embryos in the early incubation period based on RF-DS image information fusion. Machine vision and a spectrometer are used to collect information on horizontally placed embryos at day 4 of incubation. Based on image and spectral preprocessing, image texture features and spectral features are extracted. Then, basic probability assignment functions (BPAs) are constructed using the RF classification results of two single-feature types as independent evidence. Decision-level fusion is performed using D-S evidence theory, and the final identification result is given based on the classification decision threshold. Experimental results show that the highest accuracy of the image and spectral single-feature RF models reaches 78.00% and 82.67%, respectively, while the multi-feature decision fusion identification method achieves an accuracy of 88.00%, with sex identification rates of 90.00% and 86.25%, respectively. The time for identifying a single egg is 2.843 seconds. These results demonstrate that this spectral-image information fusion method can improve the accuracy of sex identification in early incubation embryos.

The invention pertains to the technical fields of bioanalytics and agriculture, and relates to a method and to an assembly for in-ovo sexing of bird eggs. The aim of the present invention is to provide a method and an assembly by means of which in-ovo sexing of bird eggs is achieved with a stable predictive accuracy of the sex and which are simple and cost-effective. The aim is achieved using a method and an assembly in which a fertilised bird egg incubated for 3 to 9 days is irradiated with at least two light-emitting laser beam sources with different excitation waves after the shell is opened, the captured and processed fluorescence scattering spectra and Raman scattering spectra being used, as complementary spectra associated with the different excitation wavelengths, to determine a classification value, and the sex being determined by comparing the determined classification value with an intrinsically determined threshold value. The method and the assembly can be used for sexing farm poultry.

Provided herein are methods of generating a sex-distinguishable insect population that include (a) delivering a cisgenic nucleic acid molecule into an insect genome, wherein the nucleic acid molecule comprises a sex-specific splicing module, and wherein the nucleic acid molecule is introduced into an endogenous phenotypic gene of the insect; (b) generating the sex-distinguishable insect population, wherein an insect of the sex-distinguishable insect population comprises the cisgenic nucleic acid molecule; and (c) sorting the insect from the sex-distinguishable insect population based on the differential sex-specific expression of the endogenous phenotypic gene, wherein the endogenous phenotypic gene is functionally expressed in only one sex.

Disclosed in the present invention are an optical sensor, a manufacturing method, and a method for rapid sex identification of a hatching egg using the optical sensor. The optical sensor comprises at least two or more sensing units in a substrate; each sensing unit comprises a gas-sensitive material capable of optical response to gas, and the gas-sensitive material is prepared into a solution, ultrasonically dispersed or dissolved, and then immobilized on the substrate to obtain the sensing unit; and the plurality of sensing units are distributed to form an array-type optical sensor. In the method, on the basis of the interaction between the gas-sensitive material of the optical sensor and the gas of the hatching egg, optical signal characteristics such as light absorption or reflection change, and then signals are collected, processed and analyzed to obtain the sex of the hatching egg. The present invention overcomes the defects of late-stage identification and invasiveness in existing hatching egg sex identification methods, and successfully enables early non-invasive determination of the sex of poultry hatching eggs.

This invention relates to the field of genetic breeding technology of *Vernicia fordii*, and discloses a CAPS molecular marker for identifying the sex of *Vernicia fordii* and its application. The nucleotide sequence of the CAPS molecular marker for identifying the sex of *Vernicia fordii* is shown in SEQ ID NO.1. The nucleotide sequences of the primer pairs for amplifying the CAPS molecular marker are shown in SEQ ID NO.2-3. The CAPS molecular marker described in this invention can efficiently and accurately distinguish between male and female *Vernicia fordii* plants at the seedling stage, solving the technical problem in the prior art where the dioecious nature of *Vernicia fordii* leads to inaccurate sex identification at the seedling stage, requiring many years to reach reproductive growth before differentiation, thus resulting in waste of land, water, fertilizer, and labor costs.

This invention discloses a method for the isolation, culture, and identification of chicken sexual gonadal cells, belonging to the field of biotechnology. The invention selects chicken embryos hatched to 18.5 days old, isolates the gonads after PCR sex identification, and uses 0.25% trypsin-EDTA digestion followed by filtration through a 70μm filter to obtain a single-cell suspension with a survival rate >90% and free from PGC contamination. A sex-specific culture system is established, allowing cells to be stably passaged and maintain a sex-specific phenotype. Through morphological, qRT-PCR, immunofluorescence, and Western blot multidimensional identification, accurate identification of male testicular Sertoli cells and female ovarian granulosa cells is achieved. This method is simple to operate, has good reproducibility, and produces high cell viability and purity. It can provide a stable gonadal microenvironment for the in vitro directed differentiation of chicken primordial germ cells, significantly improving gamete induction efficiency. It is suitable for research on avian reproductive and developmental mechanisms, transgenic breeding, and germplasm resource preservation.

The present invention provides methods of sex-sorting a plurality of insects based on sex-specific gene expression.

This invention discloses an automated system for identifying the sex of fish, belonging to the fields of aquaculture automation and machine vision technology. The automated system for identifying the sex of fish includes: a front-end section comprising a host computer, probes, and an automatic gonad identification system; and a back-end section comprising a sorting conveyor belt. The probes are used to collect fish sex characteristics and transmit the collected images or signals to the host computer. The automatic gonad identification system uses a deep learning model to perform sex identification on the collected images and outputs a sex determination result. The host computer controls the sorting conveyor belt to perform sorting operations based on the determination result. This invention aims to solve the problem of the lack of an integrated automated system in the prior art that can integrate multimodal information acquisition (such as vision and ultrasound), automatic intelligent identification, and real-time linkage with physical sorting actuators.

The invention relates to the technical field of aquatic organisms, and particularly discloses a primer pair and method for identifying the genetic sex of Chinese softshell turtles and application of the primer pair. The primer pair comprises 13448-F shown in SEQ ID NO.2 and 13448-Rshown in SEQ ID NO.3. The method comprises the steps that DNA of a sample to be detected is extracted, PCR amplification is conducted through the primer pair, and the sex is judged according to the agarose gel electrophoresis detection result. The primer pair provided by the invention can realize accurate and lossless sex identification in an embryonic stage or a juvenile soft-shelled turtle stage, and provides a key tool for genetic sex control breeding and unisexual fry production of the Chinese soft-shelled turtles.

Aspects of this invention relate to a novel monoclonal antibody specifically designed to exhibit selective binding affinity towards antigens present predominantly in X chromosome sperm. The antibody is engineered for applications to sex select sperm for cattle breeding purposes.

The application relates to a pigeon TYRP1 Mutant of a gene and application thereof, and belongs to the technical field of molecular biology. The nucleotide sequence of the mutant is shown as SEQ ID NO. 1. The application utilizes TYRP1 A frame shift mutation c.891delT (p.T297fs) of the gene has a double phenotype effect, so that it is possible to use eye color as a gender identification marker of white-feathered pigeons, and a bottleneck in application of feather color self-alternation technology in mainstream white-feathered breeds is broken.

This invention discloses a specific DNA fragment, primers, identification method, and application for sex identification in yellowfin tuna. The specific DNA fragment has the following gene sequence: male as shown in SEQ ID NO.1 and female as shown in SEQ ID NO.2. Primers were designed based on the specific DNA fragment sequence for PCR amplification and Sanger sequencing analysis. The genotype at position 219 of the male yellowfin tuna DNA fragment is T / G heterozygous, and at position 292 it is A / G heterozygous. The genotype at position 219 of the female yellowfin tuna DNA fragment is T / T homozygous, and at position 292 it is A / A homozygous. This invention successfully achieves non-lethal sex identification during the fry or juvenile stage, which is beneficial for the implementation of asexual reproduction and seedling selection in yellowfin tuna, and has significant practical value and application potential.

Methods and compositions for non-lethal biological sampling of insect larvae are described herein, as are methods and compositions for determining the sex of an insect larvae.

The application discloses a SNP molecular marker for identifying the gender of sheep and application thereof. The SNP molecular marker comprises one of SNP1, SNP2, SNP3, SNP4 and SNP5. The application provides a SNP molecular marker for identifying the gender of sheep, and the SNP molecular marker has high typing quality, single copy and high sample detection rate, and can be used for molecular marker assisted breeding of the gender of sheep. The SNP molecular marker can realize early gender typing identification of sheep embryos in a quick, high-throughput and low-cost manner, and the gender identification can be completed before embryo transplantation, so that pregnancy, delivery and feeding consumption of non-target embryos can be effectively avoided, and the breeding cycle can be directly shortened, and the selection efficiency is improved.

This invention relates to molecular marker primers, identification methods, and applications for identifying the genetic sex of pikeperch, belonging to the field of aquatic animal molecular genetics and breeding technology. This invention solves the problems of early sex identification in pikeperch, including inability to identify sex early on, high invasiveness, poor timeliness, low efficiency, and limited accuracy. It describes the application of three pairs of specific primers for identifying the genetic sex of pikeperch. The method involves either individual or combined identification, with separate amplification for combined identification, and differentiation between genetically female and male individuals based on the amplification results. The molecular markers of this invention offer multiple verification mechanisms, strong early identification capabilities, low detection costs, simple operation, non-invasiveness, high stability, wide applicability, and high throughput, making them suitable for large-scale commercial applications.

Method for Egg Sexing of Bird Embryos and Associated Devices The present invention relates to a method for egg sexing of bird embryos, the egg sexing method being implemented by a computer and comprising the steps of: - obtaining at least one image of a set of eggs for which egg sexing is to be performed, each image of an egg being a transmission image of the egg, - applying an egg sexing model to the at least one image to predict the sex of each egg in the set of eggs, the egg sexing model having been learned by training on a dataset consisting of a set of training pairs, each training pair associating the sex of the embryo with an image of an egg, the difference in incubation time between an egg from one training pair and an egg from another training pair being less than or equal to 1 hour for each training pair in the dataset. Figure for the abstract: Figure 4

Disclosed is a method of determining in-ovo gender and / or fertilisation status from an egg. The method comprises optically exciting the egg with light from a laser source; measuring autofluorescence of the egg over a period of time; calculating a fluorescence lifetime indicative of a decay of the autofluorescence over the period of time; and determining the in-ovo gender and / or fertilisation status based on the fluorescence lifetime. The fluorescence lifetime is an intrinsic property of the egg and indicative of the gender and / or fertilisation status of the egg. As such, determining the in-ovo gender and / or fertilisation status based on the fluorescence lifetime provides an accurate and non-invasive method for determining the in-ovo gender and / or fertilisation status.

This invention discloses primers, kits, and methods for rapid sex identification of the peach fruit borer based on specific molecular markers. The primer pairs are based on male-specific genes of the peach fruit borer. CpunM The design incorporates the following primer sequences: 5'-AGGCTTTCGACAAGCTTCTCTA-3' and 5'-CCTGATTTGCGTGATAAGTGGT-3'. Specific PCR detection enables reliable sex identification of peach fruit borer larvae, pupae, and adults. This invention provides efficient and reliable technical support for implementing sex-specific control strategies for peach fruit borer, including sex ratio monitoring, mating behavior research, and sterile insect technology.

This invention provides a method for breeding large yellow croaker that is entirely female and triploid. First, pseudo-male large yellow croaker germplasm is obtained using sex reversal technology. Then, pseudo-males are identified using molecular markers for sex determination. These pseudo-males are mated with normal females to obtain wholly female fertilized eggs. Hydrostatic pressure treatment is then used to inhibit the expulsion of the second polar body from these fertilized eggs, inducing the formation of wholly female triploid large yellow croaker. Simultaneously, the concentration of the sex reversal reagent, the usage cycle, water temperature, accumulated temperature, pressurization time, pressure value, and hydrostatic pressure treatment duration were all optimized to achieve a 100% triploid rate and a 100% female rate, meeting the requirements for large-scale, industrialized breeding.

The application discloses a kind of early nondestructive testing device and method of poultry egg gender based on fluorescence spectrum, solve the poor adaptability of existing poultry egg gender detection equipment, easily damage poultry egg, the problem of low accuracy, applicable to the large-scale breeding gender screening scene of poultry egg such as chicken egg, duck egg;The present application can not only realize the nondestructive, rapid, accurate detection of poultry egg gender through the collaborative design of each module, but also simple operation, cost controllable, beneficial to popularization and application, by adopting fluorescence spectrum baseline correction, median filter, smoothing processing, SNV standardization, the noise caused by test environment and equipment can be effectively eliminated, so that the characteristic peak of characteristic band is more obvious, the influence of noise on feature extraction and model training is significantly reduced, finally through standardization, the spectrum meets the requirement of batch learning.

This invention discloses a method for calculating optimal contribution for breeding and mating selection in endangered and rare cattle and sheep populations. The method includes: acquiring pedigree data and genomic information of the rare cattle and sheep; constructing a pedigree matrix based on the pedigree data; constructing a genomic kinship matrix based on the genomic information; minimizing the average kinship of the population using a genetic algorithm based on the pedigree matrix or genomic kinship matrix to determine the set of individuals to be culled; calculating the optimal contribution value of each individual using a simulated annealing algorithm for the set of individuals to be retained; and minimizing the average kinship between all pairs using a simulated annealing algorithm based on the optimal contribution values ​​of all individuals to obtain the target mating scheme. This invention balances constraints such as sex ratio and population size with kinship control, aiming to minimize the average kinship coefficient of the next generation population, greatly reducing the risk of inbreeding and ensuring population genetic diversity.