214 results about "Sexing" patented technology

The invention discloses a hyperspectral and morphological feature fused rice planthopper stage and sex identification method, and aims to improve the classification accuracy of the variety, age and sex of rice planthoppers in the initial stage of nymphs. Spectral data of rice planthoppers are obtained through a hyperspectral imaging technology, morphological characteristics are extracted by combining a morphological image processing technology, and the spectral data and morphological characteristic data are combined by adopting a multi-modal data fusion technology. And training and classifying the fused data by using a machine learning algorithm, and accurately identifying the type, age and gender of the rice planthopper. In order to solve the problem of data imbalance, an SMOTE algorithm is adopted to carry out sample oversampling, and class distribution balance is ensured. The severity of insect pests is evaluated in real time through automatic counting and population density generation, and support is provided for prevention and control measures. The screening of characteristic wave bands is expected to provide key technical support for the development of special multispectral cameras. In addition, the model interpretability is improved by adopting SHAP analysis, and the contribution of the features to the classification result is quantified.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to the weight of Beijing fatty chicken and application. The SNP molecular marker has G / A base mutation at 75bp, 655bp and 498bp of the No.4 chromosome of Beijing fatty chicken; the version number of a Beijing fatty chicken reference genome where the SNP molecular marker is located is GRCg6a. The SNP molecular marker provided by the invention can be used as a molecular marker for identifying the weight character of Beijing fatty chicken. The molecular marker provided by the invention is not limited by the age, sex and the like of Beijing fatty chicken, can be used for breeding high-weight dominant Beijing fatty chicken individuals, and greatly accelerates the breeding process of Beijing fatty chicken varieties.

The invention provides a method for breeding all-male hybrid pelteobagrus fulvidraco through hybridization of double super-male populations, which comprises the following steps: S1, respectively constructing super-male maintenance lines of pelteobagrus fulvidraco and pelteobagrus vachelli by utilizing a sex reversal technology and a sex molecular marker; s2, carrying out artificial propagation on the maintenance line, and propagating the super male fish in batches; s3, partially replacing estrogen with an androgen antagonist for the propagated supermale fish, and synergistically inducing the supermale fish to obtain superfemale fish; s4, performing specific nutrient enrichment cultivation on the super female fish and the super male parent fish; and S5, carrying out reciprocal cross artificial propagation on the super-female fish and the super-male fish between the two super-male groups to obtain a heterozygous super-male group, namely commercial fry of the all-male hybrid pelteobagrus fulvidraco. According to the technical scheme, super-female fishes can be efficiently induced in batches, high-density breeding of parent fishes is achieved, and the effect and efficiency of artificial breeding are remarkably improved; the bred hybrid all-realgar catfish is high in individual uniformity, the growth speed is increased by 20%, and the male rate reaches 100%.

The present application provides a primer combination and a kit for identifying the genetic sex of sturgeon, and a use thereof. Using two pairs of primers can not only identify the genetic sex of sturgeon in a simple and rapid manner, but also effectively avoid the experimental errors, thereby improving the accuracy of sturgeon sex identification. At the same time, the present application is not limited by the age of sturgeon and would not cause significant damage to the sturgeon. It also has good universality and may provide assistance for sex-controlled breeding of sturgeon and protection and utilization of germplasm resources.

The invention discloses a method for non-destructively identifying the gender of a transformed young duck egg based on a singing tube nuclear magnetic image, and belongs to the technical field of poultry egg detection.The method comprises the steps that a low-field nuclear magnetic resonance technology is utilized, a breeding duck egg is positioned in a nuclear magnetic system, the optimal imaging section of a singing tube is determined, an image is collected and preprocessed, and a high-quality singing tube nuclear magnetic image is obtained; and through analyzing relaxation characteristics, inverting nuclear magnetic signals to identify a buzzer tube structure and extracting characteristics, finally constructing a gender decision-making model, and combining physical constraints to obtain a gender discrimination result as a sorting reference. According to the method, lossless, early-stage and high-accuracy sex identification is realized through a special imaging technology and algorithm by utilizing sex difference nuclear magnetic characteristics of a singing tube structure, hatching and breeding strategies can be optimized in laying duck and meat duck breeding, and intelligent development of the industry is promoted.

The invention belongs to the technical field of biological medicines, discloses a preparation method of a pcDNA3.1-GH-loaded chitosan nanoparticle gene delivery system and an application of the pcDNA3.1-GH-loaded chitosan nanoparticle gene delivery system in fishes, and aims to solve the problems that the existing gene delivery technology is complicated in operation, the public are not trusted to transgenic foods and the growth rate of the fishes is low. The invention provides an oral chitosan nano-gene delivery system, which is characterized in that pcDNA3.1-GH-loaded chitosan nanoparticles are constructed by compounding a lateolabrax japonicus growth key gene gh with chitosan. Results show that the gene-loaded chitosan nanoparticles can effectively enter cells and realize the expression of exogenous genes, almost have no toxic effect, can achieve an instantaneous transgenosis effect without modifying the genome of an organism, and can significantly improve the growth performance of cultured fishes at the same time. The method has high efficiency, safety and economical efficiency, and provides a reproducible solution for industrialization of an aquaculture sex control technology.

The invention provides a cyprinus carpio sex determining gene ccMD and application thereof in cyprinus carpio sex determination or sex control, the full length of the cyprinus carpio sex determining gene ccMD is as shown in SEQ ID NO.1, the CDS sequence is as shown in SEQ ID NO.2, and the protein coded by the gene is as shown in SEQ ID NO.3. The cyprinus carpio sex determining gene ccMD can be applied to cyprinus carpio sex determination or sex control. Specific primers are designed according to the gene sequence characteristics, and XX, XY and YY carps can be rapidly and accurately identified. The gene is taken as a target gene, a gene editing technology and a gene transfer technology are adopted, hormone treatment is not needed, and a genetically male carp and a physiologically female carp or a genetically female carp and a physiologically male carp can be rapidly obtained.

The invention discloses a chicken sex identification method based on fluorescence labeling, and belongs to the technical field of gene engineering. The chicken sex identification method comprises the following steps: co-transfecting a CRISPR / Cas9 system and a WPG-EGFP donor plasmid into chicken primordial germ cells PGCs, screening to obtain EGFP positive PGCs, micro-injecting the EGFP positive PGCs into a receptor chicken embryo, hatching to obtain an EGFP reproductive chimera chicken, and identifying the sex through fluorescence labeling, the CRISPR / Cas9 system comprises sgRNA of a targeted chicken W chromosome WPG gene; the WPG-EGFP donor plasmid is constructed by inserting an EGFP gene into a WPG gene locus through a homologous recombination strategy. According to the method, the sex of the chicken can be visually and rapidly identified. The method solves the problems that a traditional sex identification method is complex in operation and depends on sample ages, has the advantages of high efficiency and accuracy, and is suitable for sex control and screening of poultry such as laying hens and broilers.

A device for determining the presence of a characteristic of a sample includes a light source for emitting pulsed excitation radiation, a detection device for detecting an autofluorescence radiation emitted by the sample, and a computer-based evaluation device. The detection device is configured to detect the autofluorescence radiation of the sample in a time-resolved manner at different wavelengths by means of time-correlated single photon counting and to provide the evaluation device with two-dimensional data with a wavelength dimension and a time dimenion. The evaluation device is configured to classify the provided data into classes by means of a classifier. The evaluation device is configured to identify specific wavelengths to be prioritized during the classification on the basis of features formed in the time dimension of the data.

The invention discloses a health monitoring and alarming system and method for animal individuals in a zoo, belongs to the field of zoo animal health monitoring, and solves the technical bottleneck through cooperation of a sensing layer, an edge computing layer and a cloud analysis layer. The system particularly sets differentiated threshold values for animals of different age groups, genders and health conditions, the dynamic baseline adjustment and multi-source data fusion algorithm is combined, the anomaly detection precision is remarkably improved, meanwhile, a grading alarm mechanism and feedback driven model optimization form a closed loop, the conversion from passive response to active early warning is achieved, and the system is suitable for the animal health monitoring and early warning of animals of different age groups, genders and health conditions. Potential health risks can be identified 12-24 hours in advance, and efficient and accurate technical support is provided for animal health management.

The invention discloses a goose gender identification method and system based on active learning and ResViT, and relates to the technical field of artificial intelligence, and the method specifically comprises the steps: collecting a goose image, and constructing an initial training data set; constructing a goose sex initial identification model; an active learning framework is adopted to initialize parameters of a goose sex initial recognition model, pre-training weights are preloaded, a hybrid sampling strategy is implemented through a dynamic data loader, a training data set is constructed based on an initial training data set in combination with sample uncertainty measurement and feature space diversity measurement, and a goose sex initial recognition model is obtained. Training the goose gender initial identification model by using the training data set to obtain a final goose gender identification model; obtaining a to-be-recognized goose image, inputting the to-be-recognized goose image into the goose gender recognition model, and outputting the goose gender; according to the method, the uncertainty and diversity of the samples are considered at the same time, the expression of the model on the aspect of unseen data can be enhanced, and the generalization ability of the model can be effectively improved.

The invention discloses a molecular specific marker for identifying genetic sex of culter alburnus and application of the molecular specific marker, and relates to the technical field of animal molecular genetics. The specific preparation method comprises the following steps: firstly, extracting culter alburnus genome DNA; then screening by utilizing the genomic DNA of the culter alburnus to obtain a culter alburnus genetic sex molecule specific marker; the method comprises the following steps: designing a primer according to a culter alburnus genetic sex molecule specific marker, and amplifying a culter alburnus genome sample to be detected; if the PCR amplification product is a single bright specific band, the individual is a female individual, and if the PCR amplification product has two bands, the individual is a male individual. The genetic sex of the culter alburnus can be quickly and accurately identified by utilizing the method, so that the method has important scientific value on the research on the sex mechanism of the culter alburnus, and has important significance and application value on the sex control and selective breeding of the culter alburnus.

The invention relates to the technical field of molecular biology and aquatic product breeding, in particular to a sex specific molecular marking method of largemouth bass and application thereof, and the sex specific molecular marking method comprises the following steps: step 1, extracting genome DNA in fin ray tissues of the largemouth bass; 2, carrying out PCR (Polymerase Chain Reaction) or qPCR (Quantitative Polymerase Chain Reaction) amplification by using a sex specific primer pair; step 3, judging the gender according to an amplification result: if a specific band of 350bp-380bp is amplified in common PCR (Polymerase Chain Reaction), or a CT (Computed Tomography) value in qPCR is less than or equal to 27, judging that the sex is male; if there is no amplified band or the CT value is greater than 27, determining that the fish is female; the primer and the probe designed based on the SVER-Y region are high in specificity and low in misjudgment rate; the application range is wide, and the method is suitable for micropterus salmoides at different growth stages; qPCR can be completed within 2 hours, and the method is suitable for large-scale sample screening.

This invention discloses an automatic weighing, sex identification, and information-based sorting system and method for crabs based on embedded control and machine vision. The system acquires weight information, ventral biotexture features, dorsal biotexture features, and micro-amplitude response features through a pre-processing acquisition link to establish an initial sorting judgment. Then, a verification label is generated and affixed through a verification label generation and affixing link. Subsequently, the verification label information is read and verification features are acquired again through a label reading and consistency verification link. Based on the consistency verification result, a decision is made on whether to proceed with formal sorting or handle the anomaly. This scheme can reduce the risk of missorting caused by mismatch between labels and actual crabs, and improve sorting accuracy and information label reliability.

The invention belongs to the technical field of molecular biology and aquaculture, and discloses a river perch sex specific molecular marker and application thereof. The molecular marker provided by the invention is directly derived from a sex specific region of the river perches and is closely linked with sex phenotypes, the identification result is accurate and reliable, and the accuracy rate can reach 100%. Enough DNA can be extracted for detection only by using a small amount of tissue samples (such as fin rays, scales, muscles or blood) of the river perches, so that the method is harmless to parent fishes, and the intactness of breeding materials is ensured. Powerful technical support is provided for all-male / all-female breeding, family construction, growth and sex relationship research and breeding production management of the river perches, and the method has a wide application prospect.

The invention relates to the technical field of molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker for predicting the daily age character of a pig for correcting 100 kg weight and application of the SNP molecular marker. The molecular marker is Ggt at the 53th site, the 157th site and the 723th site of the 12th chromosome of the Sscrofa11.1 version of a pig genome; according to the present invention, the CHD3 gene has the A single nucleotide polymorphism (SNP), and the site is located in the CHD3 gene exon region and belongs to the nonsynymous SNV; whole genome association analysis (GWAS) is carried out on a group containing 428 pigs, it is found that the site is extremely remarkably related to the growth speed required for correcting the day age of 100 kg of the weight of an individual (P = 1.27 * 10 <-10 >), and the individual carrying G allele shows higher breeding value, can serve as an important marker for molecular assisted breeding, is not limited by factors such as age and sex, and can be used as a marker for molecular assisted breeding. The method is suitable for breeding excellent boars, and can accelerate the formation of populations with excellent growth performance and improve the breeding efficiency of improved breeds.

This invention discloses a method for large-scale breeding of all-male salmon and trout, belonging to the field of fish sex regulation breeding technology. The method includes: collecting fertilized eggs from different families of the same species of salmon and trout, artificially incubating them to obtain fry, and inducing sex reversal in the fry by using 17β-estradiol when they begin feeding; subsequently, raising the fry to sexual maturity, selecting individuals with a female phenotype, and then using sdY as a sex identification gene, selecting pseudo-females with genotype XY by quantitative real-time PCR; pairing the sexually mature pseudo-females with genotype XY with normal males with genotype XY, and obtaining super-males with genotype YY through artificial insemination; pairing the sexually mature super-males with females with genotype XX, and producing all-male salmon and trout with genotype XY on a large scale through artificial insemination. This invention overcomes the problem that existing technologies cannot achieve large-scale breeding of all-male salmon and trout.

The invention relates to a sex-related SNP (Single Nucleotide Polymorphism) molecular marker of lateolabrax japonicus as well as a detection reagent and application thereof, and belongs to the technical field of fish molecular breeding. The invention provides a reagent for detecting SNP (Single Nucleotide Polymorphism) sites in a lateolabrax japonicus genome. The reagent is used for detecting genotypes of an SNP site 1, an SNP site 2 and an SNP site 3. The SNP site 1 is located at a 4806373 bp position of a chromosome 4 of a lateolabrax japonicus genome, the SNP site 2 is located at a 16421908 bp position of a chromosome 5 of the lateolabrax japonicus genome, and the SNP site 3 is located at a 7808123 bp position of a chromosome 18 of the lateolabrax japonicus genome. A group of lateolabrax japonicus sex-related SNP molecular markers are screened and used for molecular marker-assisted sex identification and early breeding of lateolabrax japonicus, and a technical tool and a reference basis are provided for rapid screening and cultivation of unisexual lateolabrax japonicus populations with better growth traits.

The invention discloses a dairy cow early embryo sex identification method, and belongs to the technical field of dairy cow breeding and breeding. The identification method comprises the following steps: (1) culturing a dairy cow embryo to an early blastocyst stage, starting to perform single-embryo single-droplet culture, and after culturing until a blastocyst cavity is formed, punching a blastocyst zona pellucida to promote the blastocyst to shrink and release blastocyst cavity liquid; (2) collecting blastocyst cavity liquid, and extracting embryo free DNA in the blastocyst cavity liquid; (3) by taking the free DNA as a template, designing a primer to amplify a specific repetitive sequence ChrY fragment of the bull Y chromosome; if the amplification product exists, the embryo is identified as a bull, and if the amplification product does not exist, the embryo is identified as a cow. The method can ensure that the embryo free DNA in the obtained blastocyst cavity fluid is only derived from the embryo, and can effectively avoid embryo injury. According to the method disclosed by the invention, the copy number of the amplified target area in the bull Y chromosome is more than 2000 times, a detection signal can be amplified, and the detection accuracy is improved.

The invention discloses a cell crude sample sex determination primer suitable for chickens, ducks or pigeons and a PCR detection method, and belongs to the technical field of biological detection. A pair of gender-specific universal primers for various poultry such as chicken, duck and pigeon is designed for the first time, a cell crude sample is adopted as a PCR template, stable amplification can be realized without DNA extraction, the operation process is greatly simplified, the detection time is shortened, and the detection cost is reduced. Meanwhile, TrypLE enzyme digestion is very mild to cells, and the method is also suitable for preparation of single-cell suspensions related to single-cell omics. The method is suitable for the fields of early sex identification of poultry, breeding poultry breeding, molecular breeding and the like, and has wide application prospects.

The embodiment of the present application relates to the technical field of breeding management, and discloses a selection and breeding method based on multidimensional data, which comprises the following steps: determining the feather characteristic parameters of chicks according to feather characteristic information and pixel point size; determining the gender and predicting the growth parameter information of the corresponding chicks according to the length of the primary wing feather and the length of the over primary wing feather, and performing primary screening according to the predicted growth parameter information; inputting the image information of the breeding hen into a feature recognition model to obtain the corresponding characteristics of the breeding hen; inputting the comb image into a comb recognition model to determine the corresponding comb type; if the feather color, the chicken toe color and the comb type match the breed parameters of the corresponding breeding hen, then the breeding hen is kept and caged, and the individual characteristic parameters are recorded. The scheme of the embodiment of the present application realizes the deep fusion of image recognition technology and multidimensional phenotype data, constructs a full-cycle accurate selection and breeding system from chicks to breeding hens, and realizes the automation of gender identification, the standardization of breed verification and the intelligentization of growth prediction.

The invention provides a sex-linked molecular marker-assisted all-male silurus asotus breeding method, which belongs to the technical field of silurus asotus sex breeding, and comprises the following steps: step 1, screening a molecular marker SSR-Y1 linked with silurus asotus Y chromosome, and the gene base sequence of the molecular marker SSR-Y1 is as shown in SEQ ID NO: 1; 2, designing a specific primer pair SSR-Y1: F: 5 '-ATGTCGATCCGAGACCT-3' and a specific primer pair SSR-Y1: R: 5 '-TGCAGATCGTCGATAGG-3', and carrying out amplification on the specific primer pair SSR-Y1: F: 5 '-ATGTCGATCGAGACCT-3'; 3, extracting the genome DNA of an individual to be detected, and amplifying the SSR-Y1 marker through PCR (Polymerase Chain Reaction); 4, judging the gender according to an amplification product, wherein male individuals show 350bp bands, and female individuals do not have bands; and 5, selecting a male parent with the genetic sex of XY, and hybridizing the male parent with the common female XX to obtain an all-male offspring XY. According to the invention, the SSR-Y1 marker is adopted, and the SSR-Y1 marker has the advantages of strong specificity, high accuracy, no consumption of a large amount of manpower and low cost; the genetic sex of the catfish is rapidly identified through PCR amplification, the production process of unisexual fish fries of the catfish is simplified by means of a molecular marker-assisted breeding technology, the breeding speed is increased, and the cost is saved.

This invention discloses a non-destructive method for sex detection of chicken embryos in the early incubation period based on RF-DS image information fusion. Machine vision and a spectrometer are used to collect information on horizontally placed embryos at day 4 of incubation. Based on image and spectral preprocessing, image texture features and spectral features are extracted. Then, basic probability assignment functions (BPAs) are constructed using the RF classification results of two single-feature types as independent evidence. Decision-level fusion is performed using D-S evidence theory, and the final identification result is given based on the classification decision threshold. Experimental results show that the highest accuracy of the image and spectral single-feature RF models reaches 78.00% and 82.67%, respectively, while the multi-feature decision fusion identification method achieves an accuracy of 88.00%, with sex identification rates of 90.00% and 86.25%, respectively. The time for identifying a single egg is 2.843 seconds. These results demonstrate that this spectral-image information fusion method can improve the accuracy of sex identification in early incubation embryos.

The invention relates to a secondary self-sexing mating method for meat quails and egg quails, and belongs to the technical field of self-sexing breeding of quails. The method comprises the following steps: (1) hybridizing an egg-type Beijing white-feather male quail and a meat-type Difark female quail to produce an F1 generation; male and female quails are identified according to the feather color of the F1 generation, the chestnut feather is a male quail, and the white feather is a female quail; or (2) hybridizing a Chinese yellow-feather male quail and a meat type Difankg female quail to produce an F1 generation; male and female quails are identified according to the F1 generation feather color, the chestnut feather is a male quail, and the yellow feather is a female quail. According to the method, Beijing white-feather quails and Chinese yellow-feather quails are respectively hybridized with meat type Difankg (FM line) quails, and the characteristics of white-feather and yellow-feather sex-accompanying inheritance are utilized to realize self-sex identification of the first generation of hybrid. And the first generation of hybrid has the characteristics of good meat production performance and high egg production performance of parents, can realize germplasm resource innovation of egg-type and meat-type quails, and has important practical application value for relieving supply and demand contradiction of high-quality quail meat in China and improving comprehensive benefits of quail breeding industry.

The invention belongs to the field of chicken hatching egg sex identification, and particularly relates to a method for identifying the sex of a chicken hatching egg by fusing a visible light image and hyperspectral data. Comprising first-stage identification and second-stage identification, in the first-stage identification, transmission light is combined with a YOLO intelligent algorithm, and fertilized egg screening and primary sex classification are achieved by identifying hatching egg blood vessel form distribution; in the second-stage identification, the form and biochemical information of the hatching eggs is collected by adopting a hyperspectral technology for secondary gender identification; if the two identification results are consistent, the female hatching eggs continue to be hatched, and the male hatching eggs are removed; if the two identification results are inconsistent, corresponding hatching eggs are collected for independent hatching. Experimental results prove that based on a two-stage method of YOLO recognition and visible light image and hyperspectral data fusion, the average accuracy of hatching egg gender classification reaches 98.9%, and an effective scheme is provided for early gender identification of chicken embryos.

The invention relates to a rapid mandarin fish sex monitoring system based on ultrasonic image feature recognition, which comprises a multi-modal data acquisition module, an integrated high-frequency ultrasonic probe (10-15MHz), a near infrared spectrum module (850nm) and a visible light camera, and is used for synchronously acquiring an ultrasonic image, blood oxygen distribution data and a mandibular contour image of a mandarin fish belly gonad area. The rapid mandarin fish sex monitoring system based on the multi-mode ultrasonic image and intelligent analysis has a high-precision identification effect, the ultrasonic texture features are combined with near-infrared blood oxygen distribution data, the sex judgment accuracy is improved to be larger than or equal to 95%, and the misjudgment rate is reduced to be smaller than 3%.

The invention belongs to the technical field of breeding of aquatic animals, and particularly relates to a breeding method for breeding XX pseudo male fishes by using Chrysophila multistriata. The invention relates to a breeding method for breeding XX pseudo-male fishes by using Christia polyrrhiza, which comprises the following steps of: feeding a feed containing an aromatase inhibitor to Christia polyrrhiza fry with proper day age for induction to obtain pseudo-male fishes with physiological male property and genetic female property; the method comprises the following steps: performing PIT living body electronic marking, then collecting a tail fin tissue sample, performing PCR detection by utilizing a genetic sex specific molecular marker of the Chrysophila polyrrhiza, and screening out pseudo male individuals with female genetic sex and male physiological sex; the obtained pseudo male fish individuals serve as male parents, normal genetic female fishes serve as female parents, intensive cultivation and artificial spawning induction are conducted, and fertilized eggs are obtained; the obtained fertilized eggs are collected and hatched, and pseudo male fishes with all genotypes being XX are obtained. According to the method, the genetic all-female rate of the breeding offspring reaches 100%, and the production purity of the offspring seeds is greatly improved.

The invention relates to the technical field of biological information processing and automatic sorting, and discloses a chick gender automatic identification method and system based on multi-modal fusion, and the method comprises the steps: collecting original three-dimensional point cloud data through a three-dimensional form sensing module, and calculating the key region coordinates of chicks; based on the coordinates, controlling the addressable micro sampling array to execute active sampling adaptation, and obtaining measurement response time sequence data of the target volatile matter; acquiring background response time sequence data by utilizing a background channel sensor array; and performing differential processing on the two to generate a differential biochemical response time sequence vector. And then, respectively extracting morphological feature vectors and biochemical fingerprint feature vectors, inputting the morphological feature vectors and the biochemical fingerprint feature vectors into respective independent classifiers to obtain prediction categories and confidence coefficients, finally, judging a bimodal prediction result through preset cross validation gating fusion logic, outputting a final decision, and controlling a sorting execution module to complete physical sorting. And the accuracy of biochemical sampling is improved through active sampling adaptation.

This invention provides a method for identifying catfish ( Silurus asotus This invention discloses a specific molecular marker for sex identification. The molecular marker is a DNA fragment located on the Y chromosome of catfish, and its nucleotide sequence is shown in SEQ ID No. 3. A DNA fragment partially homologous to this fragment is located on chromosome 8, and its nucleotide sequence is shown in SEQ ID No. 4. This invention is the first to screen male-specific fragments from catfish genome information and design specific primers for genetic sex identification of catfish. The genetic sex PCR identification method based on this molecular marker is applicable to genetic sex identification and screening of catfish at different growth stages and in different populations in different watersheds in the field, in laboratories, or in aquaculture enterprises. Since female catfish grow faster than males, applying this method to assist in the production of all-female fry helps improve the efficiency of aquaculture.

This invention discloses a specific DNA fragment, primers, and applications for sex identification of the longfin sclerotium. PCR amplification and agarose gel electrophoresis were performed using PCR primers. The results showed that individuals with a specific 271bp band were male, while those without a band were female. This invention discloses a molecular marker, primers, and identification method for sexing the longfin sclerotium. Sexing can be determined by taking fin rays without killing the fish; it can also identify the sex of juvenile fish whose sex cannot be determined by appearance. It has advantages such as accuracy, simplicity, speed, and minimal harm to the fish, providing important assistance for the conservation of wild longfin sclerotium resources and the development of the aquaculture industry.