156 results about "Sexing" patented technology

The invention provides a method for breeding all-male hybrid pelteobagrus fulvidraco through hybridization of double super-male populations, which comprises the following steps: S1, respectively constructing super-male maintenance lines of pelteobagrus fulvidraco and pelteobagrus vachelli by utilizing a sex reversal technology and a sex molecular marker; s2, carrying out artificial propagation on the maintenance line, and propagating the super male fish in batches; s3, partially replacing estrogen with an androgen antagonist for the propagated supermale fish, and synergistically inducing the supermale fish to obtain superfemale fish; s4, performing specific nutrient enrichment cultivation on the super female fish and the super male parent fish; and S5, carrying out reciprocal cross artificial propagation on the super-female fish and the super-male fish between the two super-male groups to obtain a heterozygous super-male group, namely commercial fry of the all-male hybrid pelteobagrus fulvidraco. According to the technical scheme, super-female fishes can be efficiently induced in batches, high-density breeding of parent fishes is achieved, and the effect and efficiency of artificial breeding are remarkably improved; the bred hybrid all-realgar catfish is high in individual uniformity, the growth speed is increased by 20%, and the male rate reaches 100%.

The present application provides a primer combination and a kit for identifying the genetic sex of sturgeon, and a use thereof. Using two pairs of primers can not only identify the genetic sex of sturgeon in a simple and rapid manner, but also effectively avoid the experimental errors, thereby improving the accuracy of sturgeon sex identification. At the same time, the present application is not limited by the age of sturgeon and would not cause significant damage to the sturgeon. It also has good universality and may provide assistance for sex-controlled breeding of sturgeon and protection and utilization of germplasm resources.

The invention discloses a goose gender identification method and system based on active learning and ResViT, and relates to the technical field of artificial intelligence, and the method specifically comprises the steps: collecting a goose image, and constructing an initial training data set; constructing a goose sex initial identification model; an active learning framework is adopted to initialize parameters of a goose sex initial recognition model, pre-training weights are preloaded, a hybrid sampling strategy is implemented through a dynamic data loader, a training data set is constructed based on an initial training data set in combination with sample uncertainty measurement and feature space diversity measurement, and a goose sex initial recognition model is obtained. Training the goose gender initial identification model by using the training data set to obtain a final goose gender identification model; obtaining a to-be-recognized goose image, inputting the to-be-recognized goose image into the goose gender recognition model, and outputting the goose gender; according to the method, the uncertainty and diversity of the samples are considered at the same time, the expression of the model on the aspect of unseen data can be enhanced, and the generalization ability of the model can be effectively improved.

This invention discloses an automatic weighing, sex identification, and information-based sorting system and method for crabs based on embedded control and machine vision. The system acquires weight information, ventral biotexture features, dorsal biotexture features, and micro-amplitude response features through a pre-processing acquisition link to establish an initial sorting judgment. Then, a verification label is generated and affixed through a verification label generation and affixing link. Subsequently, the verification label information is read and verification features are acquired again through a label reading and consistency verification link. Based on the consistency verification result, a decision is made on whether to proceed with formal sorting or handle the anomaly. This scheme can reduce the risk of missorting caused by mismatch between labels and actual crabs, and improve sorting accuracy and information label reliability.

The invention belongs to the technical field of molecular biology and aquaculture, and discloses a river perch sex specific molecular marker and application thereof. The molecular marker provided by the invention is directly derived from a sex specific region of the river perches and is closely linked with sex phenotypes, the identification result is accurate and reliable, and the accuracy rate can reach 100%. Enough DNA can be extracted for detection only by using a small amount of tissue samples (such as fin rays, scales, muscles or blood) of the river perches, so that the method is harmless to parent fishes, and the intactness of breeding materials is ensured. Powerful technical support is provided for all-male / all-female breeding, family construction, growth and sex relationship research and breeding production management of the river perches, and the method has a wide application prospect.

The invention relates to the technical field of molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker for predicting the daily age character of a pig for correcting 100 kg weight and application of the SNP molecular marker. The molecular marker is Ggt at the 53th site, the 157th site and the 723th site of the 12th chromosome of the Sscrofa11.1 version of a pig genome; according to the present invention, the CHD3 gene has the A single nucleotide polymorphism (SNP), and the site is located in the CHD3 gene exon region and belongs to the nonsynymous SNV; whole genome association analysis (GWAS) is carried out on a group containing 428 pigs, it is found that the site is extremely remarkably related to the growth speed required for correcting the day age of 100 kg of the weight of an individual (P = 1.27 * 10 <-10 >), and the individual carrying G allele shows higher breeding value, can serve as an important marker for molecular assisted breeding, is not limited by factors such as age and sex, and can be used as a marker for molecular assisted breeding. The method is suitable for breeding excellent boars, and can accelerate the formation of populations with excellent growth performance and improve the breeding efficiency of improved breeds.

This invention discloses a method for large-scale breeding of all-male salmon and trout, belonging to the field of fish sex regulation breeding technology. The method includes: collecting fertilized eggs from different families of the same species of salmon and trout, artificially incubating them to obtain fry, and inducing sex reversal in the fry by using 17β-estradiol when they begin feeding; subsequently, raising the fry to sexual maturity, selecting individuals with a female phenotype, and then using sdY as a sex identification gene, selecting pseudo-females with genotype XY by quantitative real-time PCR; pairing the sexually mature pseudo-females with genotype XY with normal males with genotype XY, and obtaining super-males with genotype YY through artificial insemination; pairing the sexually mature super-males with females with genotype XX, and producing all-male salmon and trout with genotype XY on a large scale through artificial insemination. This invention overcomes the problem that existing technologies cannot achieve large-scale breeding of all-male salmon and trout.

The invention relates to a sex-related SNP (Single Nucleotide Polymorphism) molecular marker of lateolabrax japonicus as well as a detection reagent and application thereof, and belongs to the technical field of fish molecular breeding. The invention provides a reagent for detecting SNP (Single Nucleotide Polymorphism) sites in a lateolabrax japonicus genome. The reagent is used for detecting genotypes of an SNP site 1, an SNP site 2 and an SNP site 3. The SNP site 1 is located at a 4806373 bp position of a chromosome 4 of a lateolabrax japonicus genome, the SNP site 2 is located at a 16421908 bp position of a chromosome 5 of the lateolabrax japonicus genome, and the SNP site 3 is located at a 7808123 bp position of a chromosome 18 of the lateolabrax japonicus genome. A group of lateolabrax japonicus sex-related SNP molecular markers are screened and used for molecular marker-assisted sex identification and early breeding of lateolabrax japonicus, and a technical tool and a reference basis are provided for rapid screening and cultivation of unisexual lateolabrax japonicus populations with better growth traits.

The invention discloses a dairy cow early embryo sex identification method, and belongs to the technical field of dairy cow breeding and breeding. The identification method comprises the following steps: (1) culturing a dairy cow embryo to an early blastocyst stage, starting to perform single-embryo single-droplet culture, and after culturing until a blastocyst cavity is formed, punching a blastocyst zona pellucida to promote the blastocyst to shrink and release blastocyst cavity liquid; (2) collecting blastocyst cavity liquid, and extracting embryo free DNA in the blastocyst cavity liquid; (3) by taking the free DNA as a template, designing a primer to amplify a specific repetitive sequence ChrY fragment of the bull Y chromosome; if the amplification product exists, the embryo is identified as a bull, and if the amplification product does not exist, the embryo is identified as a cow. The method can ensure that the embryo free DNA in the obtained blastocyst cavity fluid is only derived from the embryo, and can effectively avoid embryo injury. According to the method disclosed by the invention, the copy number of the amplified target area in the bull Y chromosome is more than 2000 times, a detection signal can be amplified, and the detection accuracy is improved.

The invention discloses a cell crude sample sex determination primer suitable for chickens, ducks or pigeons and a PCR detection method, and belongs to the technical field of biological detection. A pair of gender-specific universal primers for various poultry such as chicken, duck and pigeon is designed for the first time, a cell crude sample is adopted as a PCR template, stable amplification can be realized without DNA extraction, the operation process is greatly simplified, the detection time is shortened, and the detection cost is reduced. Meanwhile, TrypLE enzyme digestion is very mild to cells, and the method is also suitable for preparation of single-cell suspensions related to single-cell omics. The method is suitable for the fields of early sex identification of poultry, breeding poultry breeding, molecular breeding and the like, and has wide application prospects.

The embodiment of the present application relates to the technical field of breeding management, and discloses a selection and breeding method based on multidimensional data, which comprises the following steps: determining the feather characteristic parameters of chicks according to feather characteristic information and pixel point size; determining the gender and predicting the growth parameter information of the corresponding chicks according to the length of the primary wing feather and the length of the over primary wing feather, and performing primary screening according to the predicted growth parameter information; inputting the image information of the breeding hen into a feature recognition model to obtain the corresponding characteristics of the breeding hen; inputting the comb image into a comb recognition model to determine the corresponding comb type; if the feather color, the chicken toe color and the comb type match the breed parameters of the corresponding breeding hen, then the breeding hen is kept and caged, and the individual characteristic parameters are recorded. The scheme of the embodiment of the present application realizes the deep fusion of image recognition technology and multidimensional phenotype data, constructs a full-cycle accurate selection and breeding system from chicks to breeding hens, and realizes the automation of gender identification, the standardization of breed verification and the intelligentization of growth prediction.

The invention provides a sex-linked molecular marker-assisted all-male silurus asotus breeding method, which belongs to the technical field of silurus asotus sex breeding, and comprises the following steps: step 1, screening a molecular marker SSR-Y1 linked with silurus asotus Y chromosome, and the gene base sequence of the molecular marker SSR-Y1 is as shown in SEQ ID NO: 1; 2, designing a specific primer pair SSR-Y1: F: 5 '-ATGTCGATCCGAGACCT-3' and a specific primer pair SSR-Y1: R: 5 '-TGCAGATCGTCGATAGG-3', and carrying out amplification on the specific primer pair SSR-Y1: F: 5 '-ATGTCGATCGAGACCT-3'; 3, extracting the genome DNA of an individual to be detected, and amplifying the SSR-Y1 marker through PCR (Polymerase Chain Reaction); 4, judging the gender according to an amplification product, wherein male individuals show 350bp bands, and female individuals do not have bands; and 5, selecting a male parent with the genetic sex of XY, and hybridizing the male parent with the common female XX to obtain an all-male offspring XY. According to the invention, the SSR-Y1 marker is adopted, and the SSR-Y1 marker has the advantages of strong specificity, high accuracy, no consumption of a large amount of manpower and low cost; the genetic sex of the catfish is rapidly identified through PCR amplification, the production process of unisexual fish fries of the catfish is simplified by means of a molecular marker-assisted breeding technology, the breeding speed is increased, and the cost is saved.

This invention discloses a non-destructive method for sex detection of chicken embryos in the early incubation period based on RF-DS image information fusion. Machine vision and a spectrometer are used to collect information on horizontally placed embryos at day 4 of incubation. Based on image and spectral preprocessing, image texture features and spectral features are extracted. Then, basic probability assignment functions (BPAs) are constructed using the RF classification results of two single-feature types as independent evidence. Decision-level fusion is performed using D-S evidence theory, and the final identification result is given based on the classification decision threshold. Experimental results show that the highest accuracy of the image and spectral single-feature RF models reaches 78.00% and 82.67%, respectively, while the multi-feature decision fusion identification method achieves an accuracy of 88.00%, with sex identification rates of 90.00% and 86.25%, respectively. The time for identifying a single egg is 2.843 seconds. These results demonstrate that this spectral-image information fusion method can improve the accuracy of sex identification in early incubation embryos.

The invention relates to a secondary self-sexing mating method for meat quails and egg quails, and belongs to the technical field of self-sexing breeding of quails. The method comprises the following steps: (1) hybridizing an egg-type Beijing white-feather male quail and a meat-type Difark female quail to produce an F1 generation; male and female quails are identified according to the feather color of the F1 generation, the chestnut feather is a male quail, and the white feather is a female quail; or (2) hybridizing a Chinese yellow-feather male quail and a meat type Difankg female quail to produce an F1 generation; male and female quails are identified according to the F1 generation feather color, the chestnut feather is a male quail, and the yellow feather is a female quail. According to the method, Beijing white-feather quails and Chinese yellow-feather quails are respectively hybridized with meat type Difankg (FM line) quails, and the characteristics of white-feather and yellow-feather sex-accompanying inheritance are utilized to realize self-sex identification of the first generation of hybrid. And the first generation of hybrid has the characteristics of good meat production performance and high egg production performance of parents, can realize germplasm resource innovation of egg-type and meat-type quails, and has important practical application value for relieving supply and demand contradiction of high-quality quail meat in China and improving comprehensive benefits of quail breeding industry.

The invention belongs to the field of chicken hatching egg sex identification, and particularly relates to a method for identifying the sex of a chicken hatching egg by fusing a visible light image and hyperspectral data. Comprising first-stage identification and second-stage identification, in the first-stage identification, transmission light is combined with a YOLO intelligent algorithm, and fertilized egg screening and primary sex classification are achieved by identifying hatching egg blood vessel form distribution; in the second-stage identification, the form and biochemical information of the hatching eggs is collected by adopting a hyperspectral technology for secondary gender identification; if the two identification results are consistent, the female hatching eggs continue to be hatched, and the male hatching eggs are removed; if the two identification results are inconsistent, corresponding hatching eggs are collected for independent hatching. Experimental results prove that based on a two-stage method of YOLO recognition and visible light image and hyperspectral data fusion, the average accuracy of hatching egg gender classification reaches 98.9%, and an effective scheme is provided for early gender identification of chicken embryos.

The invention relates to a rapid mandarin fish sex monitoring system based on ultrasonic image feature recognition, which comprises a multi-modal data acquisition module, an integrated high-frequency ultrasonic probe (10-15MHz), a near infrared spectrum module (850nm) and a visible light camera, and is used for synchronously acquiring an ultrasonic image, blood oxygen distribution data and a mandibular contour image of a mandarin fish belly gonad area. The rapid mandarin fish sex monitoring system based on the multi-mode ultrasonic image and intelligent analysis has a high-precision identification effect, the ultrasonic texture features are combined with near-infrared blood oxygen distribution data, the sex judgment accuracy is improved to be larger than or equal to 95%, and the misjudgment rate is reduced to be smaller than 3%.

The invention belongs to the technical field of breeding of aquatic animals, and particularly relates to a breeding method for breeding XX pseudo male fishes by using Chrysophila multistriata. The invention relates to a breeding method for breeding XX pseudo-male fishes by using Christia polyrrhiza, which comprises the following steps of: feeding a feed containing an aromatase inhibitor to Christia polyrrhiza fry with proper day age for induction to obtain pseudo-male fishes with physiological male property and genetic female property; the method comprises the following steps: performing PIT living body electronic marking, then collecting a tail fin tissue sample, performing PCR detection by utilizing a genetic sex specific molecular marker of the Chrysophila polyrrhiza, and screening out pseudo male individuals with female genetic sex and male physiological sex; the obtained pseudo male fish individuals serve as male parents, normal genetic female fishes serve as female parents, intensive cultivation and artificial spawning induction are conducted, and fertilized eggs are obtained; the obtained fertilized eggs are collected and hatched, and pseudo male fishes with all genotypes being XX are obtained. According to the method, the genetic all-female rate of the breeding offspring reaches 100%, and the production purity of the offspring seeds is greatly improved.

The invention relates to the technical field of biological information processing and automatic sorting, and discloses a chick gender automatic identification method and system based on multi-modal fusion, and the method comprises the steps: collecting original three-dimensional point cloud data through a three-dimensional form sensing module, and calculating the key region coordinates of chicks; based on the coordinates, controlling the addressable micro sampling array to execute active sampling adaptation, and obtaining measurement response time sequence data of the target volatile matter; acquiring background response time sequence data by utilizing a background channel sensor array; and performing differential processing on the two to generate a differential biochemical response time sequence vector. And then, respectively extracting morphological feature vectors and biochemical fingerprint feature vectors, inputting the morphological feature vectors and the biochemical fingerprint feature vectors into respective independent classifiers to obtain prediction categories and confidence coefficients, finally, judging a bimodal prediction result through preset cross validation gating fusion logic, outputting a final decision, and controlling a sorting execution module to complete physical sorting. And the accuracy of biochemical sampling is improved through active sampling adaptation.

This invention provides a method for identifying catfish ( Silurus asotus This invention discloses a specific molecular marker for sex identification. The molecular marker is a DNA fragment located on the Y chromosome of catfish, and its nucleotide sequence is shown in SEQ ID No. 3. A DNA fragment partially homologous to this fragment is located on chromosome 8, and its nucleotide sequence is shown in SEQ ID No. 4. This invention is the first to screen male-specific fragments from catfish genome information and design specific primers for genetic sex identification of catfish. The genetic sex PCR identification method based on this molecular marker is applicable to genetic sex identification and screening of catfish at different growth stages and in different populations in different watersheds in the field, in laboratories, or in aquaculture enterprises. Since female catfish grow faster than males, applying this method to assist in the production of all-female fry helps improve the efficiency of aquaculture.

This invention discloses a specific DNA fragment, primers, and applications for sex identification of the longfin sclerotium. PCR amplification and agarose gel electrophoresis were performed using PCR primers. The results showed that individuals with a specific 271bp band were male, while those without a band were female. This invention discloses a molecular marker, primers, and identification method for sexing the longfin sclerotium. Sexing can be determined by taking fin rays without killing the fish; it can also identify the sex of juvenile fish whose sex cannot be determined by appearance. It has advantages such as accuracy, simplicity, speed, and minimal harm to the fish, providing important assistance for the conservation of wild longfin sclerotium resources and the development of the aquaculture industry.

The present application relates to a kind of Scylla paramamosain gender-specific genomic sequences and genetic gender identification method, the gender-specific genomic sequence is as SEQ NO.1 and as SEQ NO.2 shown in sequence;Wherein, genetic gender is female individual genome simultaneously containing SEQ NO.1 and SEQ NO.2 sequence, genetic gender is male individual genome only containing SEQ NO.2 sequence.Simultaneously, the present application also uses the gender-specific sequence to develop the genetic gender identification method based on PCR technology.The present application can be used for the identification of Scylla paramamosain gender-specific large fragment and genetic gender rapid detection, with the advantages of strong specificity, high accuracy, simple operation, low cost etc., can be used for crab sex control breeding industry and gender determination and differentiation mechanism research etc., with wide application prospect.

The invention relates to the field of molecular biology, and further relates to a specific marker, a primer pair, a detection method, a kit and application of macal2 gene insertion variation of oplegnathus fasciatus. The specific marker is a DNA (Deoxyribose Nucleic Acid) fragment in which the specific marker is not inserted and inserted in the intron region of the macal2 gene of the striped sea bream, and the nucleotide sequences of the DNA fragment are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. According to the invention, through a pair of specific primers, a 118bp band and a 391bp band are amplified in an individual with DNA insertion variation, and only the 118bp band is amplified in an individual without insertion variation. According to the detection method, the identification time is remarkably shortened, the detection efficiency is improved, and the method has important application value in sex identification, high-male fry preparation and family breeding of oplegnathus fasciatus.

The present application relates to a method for identifying a sex antagonistic hotspot region on a pig autosomal chromosome and application thereof. The method provided by the present application comprises the following specific steps: constructing a hybrid population F0, F1, F2 three generations, genome sequencing and SNP identification, transmission tracking of genetic material in the hybrid population pedigree, and identifying a sex antagonistic hotspot region. The method of the present application identifies that the Chr3:121M window is a sex ratio directional regulation molecular marker that is beneficial to the generation of male offspring in the Large White / Mini pig genotype individual. According to the method of the present application, the pig population sex can be predicted and regulated, and by screening and optimal mating of the genotype of the parent before mating, a plurality of generations is obtained by continuous cross without introducing exogenous blood relationship, so that the maximum value of the proportion of the genotype beneficial to the male or female is achieved under the condition that all genotypes of each generation are retained at the population level, and then the dynamic regulation of the sex ratio of the offspring pig population is achieved.

The invention provides a deep learning-based young poultry male and female identification method and system, and belongs to the field of image processing and artificial intelligence. The method comprises the following steps: acquiring a young poultry image, and preprocessing the acquired image; inputting the pre-processed young poultry image into a trained multi-target task recognition model, and performing multi-target detection and multi-target tracking on the young poultry in the image; based on a multi-target detection and tracking result, a detection branch of the multi-target task identification model is utilized to output a young poultry positioning frame, a classification branch is utilized to extract feather speed feature information and feather color feature information of young poultry in the positioning frame, and gender classification is performed based on the feather speed feature information and the feather color feature information. Outputting the gender probability of the young poultry; and summarizing and deciding the multi-frame recognition results of the same young poultry, and outputting a final sex determination result. The method can realize lossless, high-speed and high-precision identification, is high in universality, and can replace manpower to meet the requirements of a large-scale hatchery.

To provide a convenient, rapid, and reliable method for determining one or more properties of a bird egg, especially a chicken egg, and / or one or more properties of a bird embryo inside the egg, especially a chicken embryo. [Solution] A non-invasive method for determining one or more properties of a bird egg, particularly a chicken egg, and / or one or more properties of a bird embryo inside the egg, particularly a chicken embryo, using near-IR spectroscopy is described herein. More specifically, a non-invasive method for determining the sex of a bird embryo inside a bird embryo, particularly a chicken embryo, is described herein. Furthermore, a system for non-invasively determining one or more properties of a bird egg, particularly a chicken egg, and / or one or more properties of a bird embryo inside the egg, particularly a chicken embryo, is described herein, as is the use of a spectrometer selected from a multi-channel spectrometer, a miniature diffraction grating spectrometer, and a monolithic miniature spectrometer, and in particular a computer program therefor, in the system and / or method described herein.

An egg analysis system and computer-implemented methods are provided. The system comprises a light source for illuminating an egg, a photon detector for detecting a fluctuation of scattering light emitted from the egg, a processor, and a memory storing instructions which when executed by the processor configure the processor to receive scattering light data from the photon detector, digitize the scattering light data, and analyze the digitized scattering light data. One computer-implemented method comprises a light source illuminating an egg, a photon detector detecting a fluctuation of scattering light emitted from the egg, receiving scattering light data from the photon detector, digitizing the scattering light data, and analyzing the scattering light data. Another computer-implemented method comprises receiving angle, frequency and intensity data of scattering light from an egg illuminated using a light source, identifying a germinal disc in the egg from the scattering light data, and determining at least one of a fertility or a sex of the egg based on the size and structure of the germinal disc.

To provide a method for determining sex without contacting a beetle larva.SOLUTION: A sex determination method using droppings of a beetle larva includes a determination step which has unevenness of the surface of the droppings as a first index, a proportion of an area of the droppings and length of the droppings as a second index, and determines sex by combining the first index and the second index.SELECTED DRAWING: Figure 4

The invention relates to the field of animal husbandry molecular breeding and precise breeding, in particular to an animal husbandry improved variety breeding twin-fetal cattle system based on gene screening. The system comprises an ovulation potential typing module, a uterine bearing capacity quantification module, a sex compatibility verification module and a decision module. Constructing a combined evaluation model of the double-ovulation basis and the uterus metabolism capability by systematically integrating site specific amplification and gene expression quantification technologies; the core of the method is that a precise reproduction instruction is generated according to full-link data of genotypes and phenotypes; according to the method, a single ovulation rate screening mode is changed, the problem of abortion and weak abortion caused by insufficient intrauterine environment is effectively solved, and the success rate of double-fetal pregnancy is remarkably increased.

The invention discloses a large fragment deletion-based specific molecular marker for sex of spinibarbus hollandi and application of the specific molecular marker. According to the marker, whole genome re-sequencing data of male and female individuals of barbus hollandi is compared to a reference genome, and a female specific large fragment deletion region located on a No.43 chromosome (chr43) is screened out. Specific primers are designed according to the deletion region, and rapid and accurate identification of the gender of the spinibarbus hollandi can be realized through PCR amplification and electrophoretogram analysis. The method is suitable for sex identification of spinibarbus hollandi offspring seeds in the early development stage, and provides technical support for research on unisexual breeding, sex control and sex determination mechanism.