807 results about "Sexing" patented technology

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

The present invention relates to improved methods of prenatal diagnosis, screening, monitoring and / or testing. The inventive methods include the analysis by array-based hybridization of cell-free fetal DNA isolated from amniotic fluid. In addition to allowing the prenatal diagnosis of a variety of diseases and conditions, and the assessment of fetal characteristics such as fetal sex and chromosomal abnormalities, the new inventive methods provide substantially more information about the fetal genome in less time than it takes to perform a conventional metaphase karyotype analysis. In particular, the enhanced molecular karyotype methods provided by the present invention allow the detection of chromosomal aberrations that are not often detected prenatally such as microdeletions, microduplications and subtelomeric rearrangements.

The present invention relates to improved methods of prenatal diagnosis, screening, monitoring and / or testing. The inventive methods include the analysis by array-based hybridization of cell-free fetal DNA isolated from amniotic fluid. In addition to allowing the prenatal diagnosis of a variety of diseases and conditions, and the assessment of fetal characteristics such as fetal sex and chromosomal abnormalities, the new inventive methods provide substantially more information about the fetal genome in less time than it takes to perform a conventional metaphase karyotype analysis. In particular, the enhanced molecular karyotype methods provided by the present invention allow the detection of chromosomal aberrations that are not often detected prenatally such as microdeletions, microduplications and subtelomeric rearrangements. Also provided are improved methods of extraction of fetal DNA from amniotic fluid.

The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.

A method for increasing the percentage of mammalian offspring of either sex which comprises contacting a semen sample with an antibody specific for the spermatozoa determinative of one sex and separating said spermatozoa from spermatozoa determinative of the other sex, said antibody being bound to a non-porous magnetic bead support having a diameter of 0.1 to 2 microns.

A method for increasing the percentage of mammalian offspring of either sex which comprises contacting a semen sample with an antibody specific for the spermatozoa determinative of one sex and separating said spermatozoa from spermatozoa determinative of the other sex, said antibody being bound to a non-porous magnetic bead support having a diameter of 0.1 to 2 microns.

Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid and other strategies which minimize stress on the sperm cells, and, potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial application of sperms samples as well as the resulting animals may be achieved.

A hyperspectral method for detecting the present condition of an avian egg is disclosed in which a neural network algorithm is used to compare the spectrum of a test egg against a spectral library. The method can detect fertility with greater than 90% reliability on the day of laying and the gender of the chick with greater than 75% reliability on the 12th day after laying.

The invention discloses citrus fruit fly food attractant which consists of components in the following weight percent: 1 percent to 10 percent of substance with fruit aroma, 10 percent to 50 percent of solvent, 40 percent to 80 percent of nutrient and 1 percent to 10 percent of preservative. The substance with fruit aroma refers to the substance with volatile aroma extracted from fruit or that similar to the substance with volatile aroma. The attractant has very strong attracting effect; the ratio of the attracted male flies to female flies meets the citrus fruit fly fruit garden naturally group sex ratio; the attractant can obviously reduce the harm produced by egg laying of the female citrus fruit flies and effectively prevent citrus fruit flies, which is good for the continuous high-efficiency production of the fruit garden.

Following exposure to an external light source, determining: 1) the fertility of an avian egg by measuring the photon intensity (photons per second) of the egg's biophoton and luminescence; and 2) the gender of an avian egg by measuring the photon spectrum of the egg's biophoton emission and luminescence. The external light source is either an incandescent, fluorescent, LED, (pulsed or continuous wave) monochromatic or dichromatic laser light source. The detector of the photon intensity is either a low light sensing photomultiplier tube (PMT), silicon based photon counting sensor, or Geiger-mode avalanche photodiode detector. The detector of the photon spectrum is a spectrometer. Following exposure to the referenced light sources, fertile avian eggs will exhibit a higher intensity of photons than that of unfertilized avian eggs, and avian eggs of the female gender will emit a different spectrum of photons than will avian eggs of the male gender.

A non-invasive, risk-free method of prenatal diagnosis is provided. According to the method of the present invention transcervical specimens are subjected to trophoblast-specific immuno-staining followed by FISH, PRINS, Q-FISH and / or MCB analyses and / or other DNA-based genetic analysis in order to determine fetal gender and / or identify chromosomal and / or DNA abnormalities in a fetus. Also provided is a method of in situ chromosomal, DNA and / or RNA analysis of a prestained specimen by incubating the prestained specimen in ammonium hydroxide. Also provided is a method of identifying embryonic cells according to a nucleus / cytoplasm ratio of at least 1:1 and the presence of at least variably condensed chromatin.

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

The invention discloses an animal sex control method based on Rbmy gene editing and application. The method controls animal sex by using CRISPR / Cas9 system to specifically cut Rbmy gene on Y chromosome. Rbmy gene encodes a germ cell specific nucleoprotein, this protein is an important factor related to spermatogenesis, and by cutting Rbmy gene, it is possible to deactivate Rbmy gene, thus Y sperm or fertilized ovum with Y sperm is deactivated and cannot develop into a normal embryo, birth ratio of female animals is finally increased and sex control is achieved. Therefore, sex ratio of animal offspring can be controlled through the method so that the ratio of female animals with better economic value is increased and production efficiency and economic effect are greatly improved.

A means of measuring and monitoring skeletal growth rate using a plasma analyte, amino-terminal C-type natriuretic peptide (NT-CNP). Plasma NT-CNP concentrations reflect the potential for further growth of the immature skeleton. Measurement of plasma NT-CNP in a subject, when related to the mean of an appropriate age and gender matched set of control subjects, provides an index of the severity of a growth disorder not otherwise available and facilitates diagnosis in children with undetected osteo-chondrodysplasias or other intrinsic disorders of the growth plate. The invention also provides a means of detecting and monitoring potentially harmful effects of drugs and other factors on skeletal growth long before they are evident using current methods in clinical practice.

A method for feeding young white goats relates to the technical field of breeding in animal husbandry. The method for feeding the young goats in different periods after birth includes: allowing the 1-3 days aged young goats to drink colostrum as soon as possible; guaranteeing uniform sucking time of 4-7 days aged young goats to prevent diseases of 'pneumonia, enterogastritis, stomatitis and lamp dysentery'; training the 8-20 days aged young goats to eat grasses; allowing the 21-30 days aged goats to graze with ewes; allowing the 1-4 months aged young goats to feed mainly on their own; weaning the young goats when the young goats are aged from 42 days to 4 months; and grouping the weaned young goats according to sex, body weight and physique state. The method is applicable to young mutton goats which are fed by and follow the ewes for farmers or farms in agricultural areas farming Huanghuai white goats. The white goats bred by the method are high in survival rate and grow fast, breeding cycle of the white goats can be shortened, and quality of marketable goats is fine.

A method is described for treating a specimen of semen containing sperm cells to increase the relative number of sperm cells of a desired sex type in a treated specimen to increase the potential for conceiving an offspring of the desired sex. A specimen of semen is treated after a predetermined time to increase the relative ability of a at least a portion of the semen to conceive an offspring of the desired sex. The treatment preferably comprises contacting the sperm cells with an agent that preferentially effects sperm cells of a selected sex type. In some preferred embodiments, the semen is separated into two components. A first component has a higher number of sperm of the desired sex type than sperm of a non desired sex type and a second component has a higher number of sperm of the non desired sex type relative to sperm of the desired sex type. In one embodiment, the separating step is performed after a predetermined percent of the sperm cells exhibit a punctate pattern, which is capable of determination by labeling the sperm cells with Koo antibody and determining the percent of cells labeled. In another embodiment, the separating step is performed after waiting for a time determined by locating a maximum in the curve obtained by plotting percent female cells determined by FISH against percent Koo positive cells, determining the time at which the maximum percent female cells occurs, and beginning the following step no earlier than about one hour before the time of the maximum percent female cells. In yet another embodiment, the separating step is performed after waiting for a period of time between about 2 hours and about 24 hours after collection of the ejaculate. Preferably, in the separating step, the sperm is contacted with a cell binding agent, permitting the sperm of the non desired sex type to preferentially bind to the cell binding agent, and the cell binding agent with preferentially bound sperm of the non desired sex type is separated from non bound sperm.

The invention relates to a tongue sole molecule marker auxiliary sex control method, comprising the following steps: screening and clone of the female tongue sole specific AFLP marker, a construction of tongue sole genetic sex identification PCR method, preparation of pseudo male tongue sole, and feminization fingerling producing method utilizing pseudo male tongue sole; the female tongue sole specific AFLP marker is screened; the pseudo male tongue sole stimulation method and the pseudo male tongue sole identification method utilizing female specific molecule marker are constructed. The tongue sole molecule marker auxiliary sex control method firstly constructs the method that adopts the AFLP molecule marker and the sex reversing technique to control the sex of tongue sole and further to raise the feminization fry ratio, wherein, the ratio of the female fish in the tongue sole fries is increased by 20 to 25%. The tongue sole molecule marker auxiliary sex control method has the advantages of high efficiency, high accuracy, good reliability, important application value in the sex control and unisexual fingerling production of tongue sole, and potential application in the sex control and unisexual fingerling production of other fish.

The invention relates to a method and apparatus for pre-hatch avian embryo sex determination. In particular, the invention relates to a non-invasive method and apparatus for in-ovo determining the sex of avian species while in the egg and allowing to sort the eggs into groups consisting primarily of either male or female embryos. The method comprises the steps of introducing into the egg an antibody designed to match with a sex specific antigen on the embryo, which antibody is labelled, allowing the labelled antibody to migrate to and bind with the sex specific antigen on the embryo, detecting binding of the labelled antibodies on the embryo using detection means positioned outside of the egg.

The invention relates to methods for determining the sex of birds' eggs. It can be used for an early determination of the sex, in particular in poultry farming, and in this respect also with non-incubated eggs. In the method in accordance with the invention, electromagnetic radiation is emitted onto the blastodisk of an egg by a radiation source and, after a switching off of the radiation source, the decay behavior of the autofluorescence intensity excited by the electromagnetic radiation is detected by a detector at the irradiated region of the blastodisk with time resolution and spectral resolution for at least one wavelength of the autofluorescence. The fractal dimension DF is calculated using the determined measured intensity values and the value of the fractal dimension DF is compared with a species-specific and sex-specific limit value, wherein, when the limit value is exceeded, the respective egg is classified as female and, when it is not reached, the egg is classified as male.

The invention discloses a gender identification method for a chick embryo in a near-infrared hatching egg at earlier stage of incubation, and belongs to a hatching egg detection technology. The method comprises the following steps: obtaining a near-infrared spectrum of a hatching egg through an optical fiber probe and a Fourier near-infrared spectrometer in a dark room, decomposing the near-infrared spectrum of the hatching egg by an overall mean empirical mode, removing high-frequency characteristic mode components to remove noise, removing low-frequency characteristic mode components by a method based on empirical mode decomposition to achieve baseline correction, extracting main components of spectral data as input variables of a nerve network so as to finish the identification, wherein the output value of the nerve network is 0 or 1. According to the method, the gender identification efficiency and accuracy of the hatching egg at earlier stage of incubation are high, the incubation cost is reduced, and the development of layer chicken and meat chicken feeding production is facilitated.

The invention relates to a method for producing male fish, wherein it comprises that: artificially inducing; artificially inducing the female and male fishes; artificial insemination; inverting hormone; feeding seed with Artemia which contains hormone; converting male into biological female; the female grows. The invention comprises sperm inactivation and female diploid generation, to generate ready male fish and normal female fish; crosses the male fish and female fish to obtain the full male fish; inverts the male fish into biological female fish to cross with male fish, to obtain the full super male fish.

A method of testing an animal for pregnancy or sex identification comprising the steps of first, providing a first vessel containing a liquid and having a removable surface wherein said removable surface is at least partially coated with an antibody and then introducing a bodily fluid from the female animal into said first vessel so that said bodily fluid contacts the liquid and then manipulating the first vessel so that the liquid contacts the antibody. Then, a second vessel containing a reporter hormone solution is provided and the removable surface from the first vessel is displaced to the second vessel and manipulating the second vessel so that the reporter hormone solution contacts the removable surface. Then, a third vessel containing an indicating solution which has an appearance which is related to the amount of the reporter hormone contacted is provided, and the removable surface is displaced from the second vessel to the third vessel. The third vessel is manipulated so that the indicating solution contacts the removable surface. Then, a determination is made regarding the pregnancy or sex of the animal based on the appearance of the indicating solution.

Materials and Methods for the separation of X- and Y-chromosome bearing sperm, for example in a semen sample, are provided. The methods involve contacting the semen sample with a binding agent, such as an antibody, that specifically binds to an antigen that is specific for an X- or Y-chromosome. Kits for use in the methods are also provided.

A method of testing an animal for pregnancy or identifying the sex of the animal comprising the steps of first, providing a first vessel containing a liquid and having a removable surface wherein said removable surface is at least partially coated with an antibody and then introducing a bodily fluid from the female animal into said first vessel so that said bodily fluid contacts the liquid and then manipulating the first vessel so that the liquid contacts the antibody. Then, a second vessel containing a reporter hormone solution is provided and the removable surface from the first vessel is displaced to the second vessel and manipulating the second vessel so that the reporter hormone solution contacts the removable surface. Then, a third vessel containing an indicating solution which has an appearance which is related to the amount of the reporter hormone contacted is provided, and the removable surface is displaced from the second vessel to the third vessel. The third vessel is manipulated so that the indicating solution contacts the removable surface. Then, a determination is made regarding the pregnancy or sex based on the appearance of the indicating solution.

The invention relates to a gender determination method for a chicken hatching egg incubation early embryo based on a hyperspectral image, and belongs to the non-destructive testing technology in the poultry propagation industry. The gender determination method comprises the following steps: utilizing a hyperspectral imager to obtain a transmission hyperspectral image on the tenth day of chicken embryo hatching, analyzing difference of spectral response between a male embryo and a female embryo, extracting a spectral value within a wavelength range of 600-900 nm as an input value of a neural network, and judging the gender of the chicken embryo. With adoption of the method, the gender of the chicken embryo can be accurately determined, manual detection after hatching is replaced, the cost in the poultry production industry is lowered, and the production efficiency is improved.

A fish allogenous frozen-sperm induced gynogenesis method comprises a frozen storage method of the allogenous fish sperm, a genetic inactivation method, an induced method for the gynogenesis diploid fry and an identification method for the gynogenesis fry. The present invention establishes the method adopting the frozen-sperm of the sea bass to induce the egg gynogenesis of a plurality of sea fishes for the first time, which selects the cold-shock starting time, cold-shock temperature and the cold-shock durance time that are separately suitable for fish eggs of the strip-spotted flounder, the turbot and the canine-tooth paralichthys olivaceus to establish a micro-satellite marking identification method of the gynogenesis fry. The percentage rate adopting the present invention to acquire the gynogenesis fry of the strip-spotted flounder, the turbot and the canine-tooth paralichthys olivaceus is 7.8 percentages to 40.7 percentages, 26 percentages to 34 percentages and 10 percentages to 25 percentages respectively. The established method of the present invention is characterized in advancing, high efficiency, accuracy and reliability, which has an important application value in fish gender controlling and the production of the all-female fries and has a broad popularization and application prospect on fish cultivation and fish breeding.