334 results about "Sexing" patented technology

The invention relates to a pigeon individual typing detection system, a kit and application thereof. The kit comprises a pigeon individual typing detection system, the pigeon individual typing detection system comprises a primer combination and a composite amplification premix, and the primer combination comprises primers for amplifying 23 STR (short tandem repeat) microsatellite markers, bird sex detection CHD gene loci and 5 SNP (single nucleotide polymorphism) genetic markers. The pigeon individual typing detection system and the kit provided by the invention can realize single-tube detection of 29 pigeon DNA genetic markers, comprise STR genetic markers for realizing pigeon individual identification and parent-child relationship identification and single nucleotide polymorphism genetic markers for evaluating the flying ability of pigeons, and are a detection system which can be detected at present and contains the maximum number of pigeon DNA gene loci; the method has a good application prospect in the fields of pigeon identity identification, parent-child relationship identification and flight ability evaluation.

The invention discloses a method for quickly identifying the sex of a hatching egg by using an optical sensor. According to the method, an optical sensor mainly composed of a plurality of sensing units is placed in the environment near the hatching eggs for reaction, or the sensor is used for reacting with collected hatching egg gas, and then optical signal changes before and after the sensing units are placed are collected for processing and analysis to obtain the sex of the hatching eggs. Each sensing unit comprises a gas-sensitive material capable of generating optical response with gas, the gas-sensitive material is prepared into a solution, the solution is ultrasonically dispersed or dissolved and then fixed on a substrate to obtain the sensing units, and a plurality of sensing units are distributed to form the array type optical sensor. The method provided by the invention overcomes the defects of late identification and damage of the existing hatching egg sex identification method, and successfully carries out early lossless determination on the poultry hatching egg sex.

The invention belongs to the technical field of biomolecules, and particularly relates to an NIPBL gene for judging the sex of chickens and application of the NIPBL gene. The NIPBL gene has a segment with the difference of 606bp on chicken Z and W chromosomes, and is also a deletion segment on the W chromosome, the chicken sex can be quickly and effectively identified by detecting the sequence condition, the chicken with the deletion of the molecular marker sequence segment is a female individual, and the chicken without the deletion of the molecular marker sequence segment is a male individual; the invention further provides a specific primer, the chicken sex can be accurately judged through a PCR amplification technology and agarose gel electrophoresis, a strip is clear, the method is small in technical difficulty, easy and convenient to operate and high in accuracy, the sex of chicks or chickens difficult to distinguish in appearance can be effectively identified, the method has practical significance on identification of varieties small in male and female appearance difference, and the method is worthy of popularization and application. The method disclosed by the invention is strong in technical applicability and good in repeatability, can be used for accurately identifying the gender of the poultry, and has a wide application prospect.

The invention pertains to the technical fields of bioanalytics and agriculture, and relates to a method and to an assembly for in-ovo sexing of bird eggs. The aim of the present invention is to provide a method and an assembly by means of which in-ovo sexing of bird eggs is achieved with a stable predictive accuracy of the sex and which are simple and cost-effective. The aim is achieved using a method and an assembly in which a fertilised bird egg incubated for 3 to 9 days is irradiated with at least two light-emitting laser beam sources with different excitation waves after the shell is opened, the captured and processed fluorescence scattering spectra and Raman scattering spectra being used, as complementary spectra associated with the different excitation wavelengths, to determine a classification value, and the sex being determined by comparing the determined classification value with an intrinsically determined threshold value. The method and the assembly can be used for sexing farm poultry.

The invention belongs to the technical field of biomolecules, and particularly relates to an SMAD7 gene for judging chicken sex and application. Through the molecular marker and the design of a new PCR primer, the migration distance difference of two bands amplified by a ZW chromosome is 299bp, and compared with the migration distance difference of 150bp of a traditional sex identification gene CHD1 amplification system, the migration distance difference of the bands of the two items is larger, and judgment is easier; the fragments amplified by the designed PCR primer are smaller and are only 517bp and 816p, the PCR amplification time is remarkably shortened, and the sex identification time is shortened; in addition, amplification and agarose gel electrophoresis can be directly carried out by extracting chicken tissues, the sex of the chicken can be rapidly judged according to an electrophoresis result, the DNA extraction time is shortened, the time length can be controlled within 1 hour, operation is easy, the identification result is accurate, basic-level application and popularization are facilitated, and the method has a wide market application prospect.

The invention discloses siRNA for intervening sex proportion of haemonchus contortus population and application, and relates to the field of animal parasitic disease prevention and treatment. In-vivo tests of ruminant Hu sheep prove that the daily ovulation amount of an insect attacking group after the siRNA primer is applied is remarkably reduced, the ovulation peak period is delayed by 4 days compared with that of a normal group, the insect load amount of adults (especially female insects) is remarkably reduced, and a certain propagation blocking effect is achieved.

The invention discloses application of luteolin in bullfrog culture, promotion of bullfrog tadpole metamorphosis development and sex differentiation regulation. The luteolin is utilized to effectively shorten the metamorphosis development period of bullfrog tadpoles into froglets, accelerate the metamorphosis development of the bullfrog tadpoles, improve the success rate of the metamorphosis development of the bullfrog tadpoles, improve the breeding efficiency, promote the male differentiation of the bullfrog tadpoles and increase the yield of male bullfrogs; the method has the remarkable advantages of greenness, safety, simplicity and convenience in operation and stable effect. The invention provides a new technical choice for bullfrog breeding industry, which is beneficial to optimizing germplasm resources of bullfrogs and improving economic benefits.

The invention discloses a hyperspectral and morphological feature fused rice planthopper stage and sex identification method, and aims to improve the classification accuracy of the variety, age and sex of rice planthoppers in the initial stage of nymphs. Spectral data of rice planthoppers are obtained through a hyperspectral imaging technology, morphological characteristics are extracted by combining a morphological image processing technology, and the spectral data and morphological characteristic data are combined by adopting a multi-modal data fusion technology. And training and classifying the fused data by using a machine learning algorithm, and accurately identifying the type, age and gender of the rice planthopper. In order to solve the problem of data imbalance, an SMOTE algorithm is adopted to carry out sample oversampling, and class distribution balance is ensured. The severity of insect pests is evaluated in real time through automatic counting and population density generation, and support is provided for prevention and control measures. The screening of characteristic wave bands is expected to provide key technical support for the development of special multispectral cameras. In addition, the model interpretability is improved by adopting SHAP analysis, and the contribution of the features to the classification result is quantified.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to the weight of Beijing fatty chicken and application. The SNP molecular marker has G / A base mutation at 75bp, 655bp and 498bp of the No.4 chromosome of Beijing fatty chicken; the version number of a Beijing fatty chicken reference genome where the SNP molecular marker is located is GRCg6a. The SNP molecular marker provided by the invention can be used as a molecular marker for identifying the weight character of Beijing fatty chicken. The molecular marker provided by the invention is not limited by the age, sex and the like of Beijing fatty chicken, can be used for breeding high-weight dominant Beijing fatty chicken individuals, and greatly accelerates the breeding process of Beijing fatty chicken varieties.

The invention provides a breeding method of all-male pseudosciaena crocea and a matched preservation line, and relates to the technical field of pseudosciaena crocea breeding. According to the all-male larimichthys crocea, YY type male larimichthys crocea and XX type female larimichthys crocea are paired, and all produced offspring are normal XY type male larimichthys crocea. The cultivation method of the large yellow croaker matched preservation line comprises the following steps: firstly, performing induced transformation on male large yellow croaker by using estrogen to obtain XY type female large yellow croaker; then the XY type female pseudosciaena crocea subjected to sexual transformation is matched with normal XY type male pseudosciaena crocea, and produced offspring contains YY type male genetic pseudosciaena crocea; and pairing the YY type male genetic large yellow croaker with the normal XX type female large yellow croaker, wherein all the produced offspring are the normal XY type male large yellow croaker. Through the application of estrogen induction and genetic sex identification technologies, the sex control accuracy of the large yellow croaker is realized, and a new technical means is provided for the aquaculture industry.

The invention provides a method for breeding all-male hybrid pelteobagrus fulvidraco through hybridization of double super-male populations, which comprises the following steps: S1, respectively constructing super-male maintenance lines of pelteobagrus fulvidraco and pelteobagrus vachelli by utilizing a sex reversal technology and a sex molecular marker; s2, carrying out artificial propagation on the maintenance line, and propagating the super male fish in batches; s3, partially replacing estrogen with an androgen antagonist for the propagated supermale fish, and synergistically inducing the supermale fish to obtain superfemale fish; s4, performing specific nutrient enrichment cultivation on the super female fish and the super male parent fish; and S5, carrying out reciprocal cross artificial propagation on the super-female fish and the super-male fish between the two super-male groups to obtain a heterozygous super-male group, namely commercial fry of the all-male hybrid pelteobagrus fulvidraco. According to the technical scheme, super-female fishes can be efficiently induced in batches, high-density breeding of parent fishes is achieved, and the effect and efficiency of artificial breeding are remarkably improved; the bred hybrid all-realgar catfish is high in individual uniformity, the growth speed is increased by 20%, and the male rate reaches 100%.

The present application provides a primer combination and a kit for identifying the genetic sex of sturgeon, and a use thereof. Using two pairs of primers can not only identify the genetic sex of sturgeon in a simple and rapid manner, but also effectively avoid the experimental errors, thereby improving the accuracy of sturgeon sex identification. At the same time, the present application is not limited by the age of sturgeon and would not cause significant damage to the sturgeon. It also has good universality and may provide assistance for sex-controlled breeding of sturgeon and protection and utilization of germplasm resources.

The invention discloses a chicken embryo sex determination kit directly using blood as a template and application thereof. Belongs to the technical field of biology. The kit comprises Taq DNA polymerase, an upstream primer, a downstream primer and water. Compared with the prior art, the method has the beneficial effects that a PCR reaction system is optimized by adopting the super-fidelity Taq DNA polymerase, whole blood is diluted and then is directly used as a template for PCR reaction, tedious DNA extraction is not needed, meanwhile, a PCR reaction enhancer and a PCR system stabilizer are not needed to be added into the reaction system, and the operation is simple and convenient. The method has the advantages of simple and convenient operation, less time consumption, rapid identification and the like, and avoids the defects of time and labor consumption and error proneness of a traditional method.

The invention discloses a method for non-destructively identifying the gender of a transformed young duck egg based on a singing tube nuclear magnetic image, and belongs to the technical field of poultry egg detection.The method comprises the steps that a low-field nuclear magnetic resonance technology is utilized, a breeding duck egg is positioned in a nuclear magnetic system, the optimal imaging section of a singing tube is determined, an image is collected and preprocessed, and a high-quality singing tube nuclear magnetic image is obtained; and through analyzing relaxation characteristics, inverting nuclear magnetic signals to identify a buzzer tube structure and extracting characteristics, finally constructing a gender decision-making model, and combining physical constraints to obtain a gender discrimination result as a sorting reference. According to the method, lossless, early-stage and high-accuracy sex identification is realized through a special imaging technology and algorithm by utilizing sex difference nuclear magnetic characteristics of a singing tube structure, hatching and breeding strategies can be optimized in laying duck and meat duck breeding, and intelligent development of the industry is promoted.

The invention belongs to the technical field of biological medicines, discloses a preparation method of a pcDNA3.1-GH-loaded chitosan nanoparticle gene delivery system and an application of the pcDNA3.1-GH-loaded chitosan nanoparticle gene delivery system in fishes, and aims to solve the problems that the existing gene delivery technology is complicated in operation, the public are not trusted to transgenic foods and the growth rate of the fishes is low. The invention provides an oral chitosan nano-gene delivery system, which is characterized in that pcDNA3.1-GH-loaded chitosan nanoparticles are constructed by compounding a lateolabrax japonicus growth key gene gh with chitosan. Results show that the gene-loaded chitosan nanoparticles can effectively enter cells and realize the expression of exogenous genes, almost have no toxic effect, can achieve an instantaneous transgenosis effect without modifying the genome of an organism, and can significantly improve the growth performance of cultured fishes at the same time. The method has high efficiency, safety and economical efficiency, and provides a reproducible solution for industrialization of an aquaculture sex control technology.

The invention provides a cyprinus carpio sex determining gene ccMD and application thereof in cyprinus carpio sex determination or sex control, the full length of the cyprinus carpio sex determining gene ccMD is as shown in SEQ ID NO.1, the CDS sequence is as shown in SEQ ID NO.2, and the protein coded by the gene is as shown in SEQ ID NO.3. The cyprinus carpio sex determining gene ccMD can be applied to cyprinus carpio sex determination or sex control. Specific primers are designed according to the gene sequence characteristics, and XX, XY and YY carps can be rapidly and accurately identified. The gene is taken as a target gene, a gene editing technology and a gene transfer technology are adopted, hormone treatment is not needed, and a genetically male carp and a physiologically female carp or a genetically female carp and a physiologically male carp can be rapidly obtained.

The invention discloses a method for constructing gene-free edited all-male fish based on a kdm6bb gene knock-down technology, which comprises the following steps: carrying out knock-down treatment on a fish kdm6bb gene by utilizing a CRISPR / Cas9 gene editing technology, and carrying out genotype identification to obtain a kdm6bb heterozygote (kdm6bb + / -); a kdm6bb heterozygous mutant capable of effectively reducing kdm6bb protein expression is obtained through selfing of heterozygous fish; pseudo female fishes with XY sex genetic types are screened through sex molecular markers, the pseudo female fishes are mated with wild XY male fishes, YY sex genetic super-male fishes without kdm6bb mutation are screened, and the YY sex genetic super-male fishes are hybridized with wild XX female fishes, so that all-male fish groups without kdm6bb mutation are obtained, unisexual culture of fishes is realized, sex control breeding efficiency is improved, and breeding cost is reduced. The present invention is widely applicable to aquatic economic fishes on the basis of XX / XY sex determination.

A method for generating a standard growth curve for chicken flock breeding comprises the following specific steps that a, the same kind of chicken flock is divided into N groups to be bred, and it is guaranteed that all chickens in each group of chicken flock are born on the same day; b, recording the daily average weight of each group of chicken flocks; c, taking the daily average weight of all groups of chicken flocks as a training set, learning in a learning model, averaging, and finally generating a daily age average weight standard growth curve of the same type of chicken flocks by means of a Gompertz fitting model; and d, changing any condition of variety, gender, region, henhouse control mode, climate and breeding mode to form a new type of chicken flocks, and repeating the steps b and c to obtain daily age average weight standard growth curves of different types of chicken flocks. The daily age average weight standard growth curve obtained by the method is more consistent with the actual weight of the chicken flocks, the average weight prediction of the chicken flocks is more scientific and accurate, and meanwhile, the problem of physical health of the chickens caused by a mode of catching the chickens by sampling is avoided.

The invention discloses a chicken sex identification method based on fluorescence labeling, and belongs to the technical field of gene engineering. The chicken sex identification method comprises the following steps: co-transfecting a CRISPR / Cas9 system and a WPG-EGFP donor plasmid into chicken primordial germ cells PGCs, screening to obtain EGFP positive PGCs, micro-injecting the EGFP positive PGCs into a receptor chicken embryo, hatching to obtain an EGFP reproductive chimera chicken, and identifying the sex through fluorescence labeling, the CRISPR / Cas9 system comprises sgRNA of a targeted chicken W chromosome WPG gene; the WPG-EGFP donor plasmid is constructed by inserting an EGFP gene into a WPG gene locus through a homologous recombination strategy. According to the method, the sex of the chicken can be visually and rapidly identified. The method solves the problems that a traditional sex identification method is complex in operation and depends on sample ages, has the advantages of high efficiency and accuracy, and is suitable for sex control and screening of poultry such as laying hens and broilers.

The invention relates to a specific primer pair for sex identification of Chinese softshell turtles and an identification method thereof.The primer pair comprises two pairs of primers which are used for amplifying specific sequences in genome DNA of the Chinese softshell turtles respectively so as to achieve accurate sex identification, and according to the method, the genome DNA of a to-be-detected sample can be amplified through a PCR amplification reaction; when the amplification product is 1000 bp, the sex is judged, when the amplification product is 1000 bp and 2000 bp, the sex is judged, according to the identification method provided by the invention, standard PCR amplification conditions and a reaction system are adopted, high efficiency and accuracy of sex identification are ensured, the invention further provides a kit, and the kit can be used for detecting the sex. The kit comprises a reagent for extracting DNA (deoxyribonucleic acid), a primer combination and a reaction system required by PCR (polymerase chain reaction) amplification as well as an electrophoretic buffer solution and a labeling reagent required by agarose gel electrophoresis, is beneficial to improving the sex identification efficiency and accuracy of the Chinese softshell turtles, and is widely applicable to sex screening and other related researches in the aquaculture industry.

A device for determining the presence of a characteristic of a sample includes a light source for emitting pulsed excitation radiation, a detection device for detecting an autofluorescence radiation emitted by the sample, and a computer-based evaluation device. The detection device is configured to detect the autofluorescence radiation of the sample in a time-resolved manner at different wavelengths by means of time-correlated single photon counting and to provide the evaluation device with two-dimensional data with a wavelength dimension and a time dimenion. The evaluation device is configured to classify the provided data into classes by means of a classifier. The evaluation device is configured to identify specific wavelengths to be prioritized during the classification on the basis of features formed in the time dimension of the data.

The invention discloses a loach sex-specific SNP (Single Nucleotide Polymorphism) marker and a genetic sex identification method, and belongs to the technical field of fish genetic breeding. The loach sex specificity SNP marker disclosed by the invention is obtained through whole genome re-sequencing and GWAS analysis and screening; according to the loach genetic sex identification method, on the basis that specific primers are designed aiming at the SNP marker, loach genome DNA is used as a template, individual SNP types are obtained through PCR amplification and Sanger sequencing, the homozygous type is female, and the heterozygous type is male. According to the method, the genetic sex of the individual can be accurately identified, pseudo male fish, pseudo female fish and the like obtained through artificial induction can be screened out, sex identification can be carried out in the early development stage of the individual, and the method has the advantages that damage of the tested individual is minimized, and survival is not affected. The method has an important application value in parent sex identification in loach sex control breeding.

The invention discloses a health monitoring and alarming system and method for animal individuals in a zoo, belongs to the field of zoo animal health monitoring, and solves the technical bottleneck through cooperation of a sensing layer, an edge computing layer and a cloud analysis layer. The system particularly sets differentiated threshold values for animals of different age groups, genders and health conditions, the dynamic baseline adjustment and multi-source data fusion algorithm is combined, the anomaly detection precision is remarkably improved, meanwhile, a grading alarm mechanism and feedback driven model optimization form a closed loop, the conversion from passive response to active early warning is achieved, and the system is suitable for the animal health monitoring and early warning of animals of different age groups, genders and health conditions. Potential health risks can be identified 12-24 hours in advance, and efficient and accurate technical support is provided for animal health management.

A sexing service-providing system 10 includes irradiation means for irradiating each of hen eggs within a predetermined period of time with light having a predetermined wavelength, imaging means for imaging each of the irradiated hen eggs, means for generating a sexing model using, as training data, a result of separately sexing hen eggs and image data obtained by the imaging of the hen eggs, means for sexing a new target hen egg by inputting the image data obtained for the target hen egg by the irradiation means and the imaging means into the sexing model, discharging means for notifying a control mechanism of an incubator of a result of the sexing and causing the incubator to continue incubation of the hen egg when determined to be a female egg by the sexing, and perform a process of discharging the hen egg when determined to be a male egg by the sexing from the incubator.

The invention discloses a goose gender identification method and system based on active learning and ResViT, and relates to the technical field of artificial intelligence, and the method specifically comprises the steps: collecting a goose image, and constructing an initial training data set; constructing a goose sex initial identification model; an active learning framework is adopted to initialize parameters of a goose sex initial recognition model, pre-training weights are preloaded, a hybrid sampling strategy is implemented through a dynamic data loader, a training data set is constructed based on an initial training data set in combination with sample uncertainty measurement and feature space diversity measurement, and a goose sex initial recognition model is obtained. Training the goose gender initial identification model by using the training data set to obtain a final goose gender identification model; obtaining a to-be-recognized goose image, inputting the to-be-recognized goose image into the goose gender recognition model, and outputting the goose gender; according to the method, the uncertainty and diversity of the samples are considered at the same time, the expression of the model on the aspect of unseen data can be enhanced, and the generalization ability of the model can be effectively improved.

The invention belongs to the technical field of gene detection, and particularly discloses an STR fluorescent multiplex amplification detection kit for pigeon individual recognition, a detection method and application of the STR fluorescent multiplex amplification detection kit. The kit comprises 19 STR (short tandem repeat) loci and one sex identification locus of the domestic pigeon, also comprises an allele lader and the like, can more accurately judge the typing of the loci, has high cumulative paternity exclusion rate, can be applied to individual identification and paternity relationship identification of the domestic pigeon, has high detection sensitivity, and can obtain an accurate typing result only by 0.5 ng of DNA (deoxyribonucleic acid).

The invention discloses a molecular specific marker for identifying genetic sex of culter alburnus and application of the molecular specific marker, and relates to the technical field of animal molecular genetics. The specific preparation method comprises the following steps: firstly, extracting culter alburnus genome DNA; then screening by utilizing the genomic DNA of the culter alburnus to obtain a culter alburnus genetic sex molecule specific marker; the method comprises the following steps: designing a primer according to a culter alburnus genetic sex molecule specific marker, and amplifying a culter alburnus genome sample to be detected; if the PCR amplification product is a single bright specific band, the individual is a female individual, and if the PCR amplification product has two bands, the individual is a male individual. The genetic sex of the culter alburnus can be quickly and accurately identified by utilizing the method, so that the method has important scientific value on the research on the sex mechanism of the culter alburnus, and has important significance and application value on the sex control and selective breeding of the culter alburnus.

The invention relates to the technical field of molecular biology and aquatic product breeding, in particular to a sex specific molecular marking method of largemouth bass and application thereof, and the sex specific molecular marking method comprises the following steps: step 1, extracting genome DNA in fin ray tissues of the largemouth bass; 2, carrying out PCR (Polymerase Chain Reaction) or qPCR (Quantitative Polymerase Chain Reaction) amplification by using a sex specific primer pair; step 3, judging the gender according to an amplification result: if a specific band of 350bp-380bp is amplified in common PCR (Polymerase Chain Reaction), or a CT (Computed Tomography) value in qPCR is less than or equal to 27, judging that the sex is male; if there is no amplified band or the CT value is greater than 27, determining that the fish is female; the primer and the probe designed based on the SVER-Y region are high in specificity and low in misjudgment rate; the application range is wide, and the method is suitable for micropterus salmoides at different growth stages; qPCR can be completed within 2 hours, and the method is suitable for large-scale sample screening.

This invention discloses an automatic weighing, sex identification, and information-based sorting system and method for crabs based on embedded control and machine vision. The system acquires weight information, ventral biotexture features, dorsal biotexture features, and micro-amplitude response features through a pre-processing acquisition link to establish an initial sorting judgment. Then, a verification label is generated and affixed through a verification label generation and affixing link. Subsequently, the verification label information is read and verification features are acquired again through a label reading and consistency verification link. Based on the consistency verification result, a decision is made on whether to proceed with formal sorting or handle the anomaly. This scheme can reduce the risk of missorting caused by mismatch between labels and actual crabs, and improve sorting accuracy and information label reliability.

The invention belongs to the technical field of molecular biology and aquaculture, and discloses a river perch sex specific molecular marker and application thereof. The molecular marker provided by the invention is directly derived from a sex specific region of the river perches and is closely linked with sex phenotypes, the identification result is accurate and reliable, and the accuracy rate can reach 100%. Enough DNA can be extracted for detection only by using a small amount of tissue samples (such as fin rays, scales, muscles or blood) of the river perches, so that the method is harmless to parent fishes, and the intactness of breeding materials is ensured. Powerful technical support is provided for all-male / all-female breeding, family construction, growth and sex relationship research and breeding production management of the river perches, and the method has a wide application prospect.