The invention comprises a method to predict iris color of a human from a 
nucleic acid / 
protein sample comprising assaying for one or more polymorphisms in the region 5′ proximal of the OCA2 
gene up to and including the HERC2 
gene on 
chromosome 15 between basepairs 26018062 and 26240890 according to NCBI build 36 or Ensemble Homo sapiens version 46.36h and on basis of the results from the 
assay predicting the 
eye color of a human e.g. an unknown person (such as perpetrators and / or victims of crime, missing persons etc.) in forensic and other applications of human identification. Said polymorphisms preferably are selected from the group consisting of rs916977, rs8028689, rs6497287, rs8041209, rs6497292, rs2240202, rs2346050, rs12592730, rs7183877, rs2240204, rs8039195, rs16950979, rs16950987, rs1667394, and rs1635168 or any marker in close physical distance to said markers and consequently in 
genetic linkage in the region of 
chromosome 15 between basepairs 26018062 and 26240890 according to NCBI build 36 or Ensemble Homo sapiens version 46.36h. Said polymorphisms are analysed from 
human material such as 
human body fluids (e.g. blood, 
saliva, 
semen etc.) or other 
human body parts (e.g. hairs, organs, etc) or from material obtained from whole bodies. The invention further comprises primers and probes, and a kit for the 
assay. The invention includes application of the genetic 
eye color prediction using said markers or their combinations (haplotypes) for forensic and other purposes of human identification such as to identify or trace unknown persons e.g. perpetrators and / or victims of crime, missing persons etc.