52 results about "Genetic linkage" patented technology

A method for diagnosis of a mood disorder or predisposition therefor in a test subject is disclosed. The method includes the steps of determining binocular rivalry rate in the test subject and comparing the binocular rivalry rate with a corresponding reference rivalry rate to diagnose presence or absence of the mood disorder or predisposition therefor. Also disclosed is use of the diagnostic method in genetic linkage studies for the identification of the molecular defect(s) underlying these disorders, and for the identification of compounds which may alleviate such disorders.

A method and apparatus for diagnosing schizophrenia, schizophrenia disorder subgroup, or predisposition thereto in a test subject is disclosed. The method includes the steps of determining an interhemispheric switch rate of the test subject. In one embodiment, the interhemispheric switch rate of the test subject is under conditions of increasing rate of dichoptic reversal, and comparing the switch rate with a corresponding reference switch rate to diagnose presence or absence of schizophrenia, a schizophrenic disorder subgroup, or predisposition thereto. In a preferred embodiment, the interhemispheric switch rate is determined by measuring the rate of binocular rivalry in the test subject. Also disclosed is use of a diagnostic method in genetic linkage studies for the identification of the molecular defect(s) underlying schizophrenia, and for the identification of compounds which may alleviate the disorder.

A method for predicting harvestable biomass yield in a crop comprising: genotyping a sample obtained from a crop plant for one or more markers genetically linked to a polynucleotide sequence having at least 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 97, 98, 99 or 100% identity to SEQ BD NO 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25, whereby the markers individually or collectively identify a haplotype associated with yield in a plurality of crop plants and correlating the haplotype with the harvested biomass yield.

The invention belongs to the field of innovation and research of plant genetics breeding and apple germplasm, in particular to an SSR (simple sequence repeat) molecular marker III for identifying descendant plants of Gala apple and application thereof. The SSR molecular marker III is characterized in that in the detection process, the SSR molecular markers linked with No.4, No.6, No.12 and No.14 chromosomes are simultaneously used, DNA (deoxyribonucleic acid) of an apple gene group is used as a PCR (polymerase chain reaction) amplification template, the SSR molecular markers are respectively used as primer pairs, and the PCR amplification and product detection are performed, so as to identify the genetics linkage, anther culture plants and variety sources. The SSR molecular marker III has the advantages that the SSR molecular marker is used for identifying the apple linkage group of the identifying material for the first time, and the SSR molecular markers linked with No.4, No.6, No.12 and No.14 chromosomes of the apple are disclosed; the molecular marker is a co-dominant marker, so that the Gala apple genetic linkage group, the anther culture plant and the Gala source plant are quickly and accurately identified; the molecular level support is provided for further accelerating the utilization of the Gala apple and important agronomic trait linkage gene, and the genetic breeding of apple homozygous plants.

The invention belongs to the field of innovation and research of plant genetics breeding and apple germplasm, in particular to an SSR (simple sequence repeat) molecular marker IV for identifying descendant plants of Gala apple and application thereof. The SSR molecular marker IV is characterized in that in the detection process, the SSR molecular markers linked with No.5 and No.10 chromosomes are simultaneously used, DNA (deoxyribonucleic acid) of an apple gene group is used as a PCR (polymerase chain reaction) amplification template, the SSR molecular markers are respectively used as primer pairs, and the PCR amplification and product detection are performed, so as to identify the genetics linkage, anther culture plants and variety sources. The SSR molecular marker IV has the advantages that the SSR molecular marker is used for identifying the apple linkage group of the identifying material for the first time, and the SSR molecular markers linked with No.5 and No.10 chromosomes of the apple are disclosed; the molecular marker is a co-dominant marker, so that the Gala apple genetic linkage group, the anther culture plant and the Gala source plant are quickly and accurately identified; the molecular level support is provided for further accelerating the utilization of the Gala apple and important agronomic trait linkage gene, and the genetic breeding of apple homozygous plants.

The invention comprises a method to predict iris color of a human from a nucleic acid / protein sample comprising assaying for one or more polymorphisms in the region 5′ proximal of the OCA2 gene up to and including the HERC2 gene on chromosome 15 between basepairs 26018062 and 26240890 according to NCBI build 36 or Ensemble Homo sapiens version 46.36h and on basis of the results from the assay predicting the eye color of a human e.g. an unknown person (such as perpetrators and / or victims of crime, missing persons etc.) in forensic and other applications of human identification. Said polymorphisms preferably are selected from the group consisting of rs916977, rs8028689, rs6497287, rs8041209, rs6497292, rs2240202, rs2346050, rs12592730, rs7183877, rs2240204, rs8039195, rs16950979, rs16950987, rs1667394, and rs1635168 or any marker in close physical distance to said markers and consequently in genetic linkage in the region of chromosome 15 between basepairs 26018062 and 26240890 according to NCBI build 36 or Ensemble Homo sapiens version 46.36h. Said polymorphisms are analysed from human material such as human body fluids (e.g. blood, saliva, semen etc.) or other human body parts (e.g. hairs, organs, etc) or from material obtained from whole bodies. The invention further comprises primers and probes, and a kit for the assay. The invention includes application of the genetic eye color prediction using said markers or their combinations (haplotypes) for forensic and other purposes of human identification such as to identify or trace unknown persons e.g. perpetrators and / or victims of crime, missing persons etc.

The present invention addresses the problem of providing a simple and efficient means for predicting the risk of occurrence of a side effect of irinotecan by analyzing a single nucleotide polymorphism in a region encoding a specific gene. The prediction of the risk of occurrence of a side effect of irinotecan is assisted by analyzing: a single nucleotide polymorphism in a region encoding the APCDD1L gene, the R3HCC1 gene, the OR51I2 gene, the MKKS gene, the EDEM3 gene, or the ACOX1 gene which are present on genomic DNA in a biological sample collected from a subject; or a single nucleotide polymorphism which is in linkage disequilibrium with or genetically linked to the single nucleotide polymorphism, thereby determining whether the single nucleotide polymorphism is homozygous or heterozygous for a variant-type, or homozygous for a wild-type and assisting prediction of the risk of occurrence of a side effect of irinotecan.

The invention provides a construction method of a parting High Map on the basis of high throughput. The construction method comprises the steps as follows: 1), genetic segregation population markers subjected to development and parting with a high-throughput sequencing method; 2), every two markers are subjected to genetic linkage test, and linkage groups are divided; 3), linear ordering is performed and the genetic distance is calculated with an SGS algorithm, and parting errors and parting loss in sample parting data are subjected to error correction and loss compensation with a KNN algorithm; and 4), the constructed map is subjected to accuracy evaluation, and the map quality is directly displayed with a visual method. According to the construction method of the parting High Map, the parting errors and the parting loss caused by high-throughput sequencing parting are effectively eliminated through parting error correction, and the accuracy of the constructed map is improved remarkably; the SGS sequencing algorithm is used, so that the sequencing speed is high, a High Map with more than one thousand markers in a single linkage group can be constructed, and the map drawing efficiency is improved remarkably; and the requirement for original parting data is reduced, and the parting error tolerance is improved greatly.