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Linked molecular marker for powdery mildew resistant gene pm1 of cucurbita pepo L. and application of linked molecular marker

A technology of disease-resistant genes and molecular markers, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as no research reports on zucchini, and achieve convenient identification, stable amplification, and accelerated utilization Effect

Active Publication Date: 2015-04-08
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on molecular markers linked to melon powdery mildew resistance is mainly concentrated on watermelon, melon and cucumber, and there is no relevant research report on zucchini at home and abroad.

Method used

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  • Linked molecular marker for powdery mildew resistant gene pm1 of cucurbita pepo L. and application of linked molecular marker
  • Linked molecular marker for powdery mildew resistant gene pm1 of cucurbita pepo L. and application of linked molecular marker
  • Linked molecular marker for powdery mildew resistant gene pm1 of cucurbita pepo L. and application of linked molecular marker

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Experimental program
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Embodiment 1

[0032] Dominant resistance gene to powdery mildew of zucchini Pm1 The linked SSR molecular marker, said molecular marker is Pm-136, which has the DNA sequence described in SEQ ID NO. 1 and SEQ ID NO. 2 in the sequence listing.

[0033] The zucchini powdery mildew dominant disease resistance gene Pm1 A method for obtaining linked SSR molecular markers, comprising the following steps:

[0034] Application of SSR-BSA technology: using zucchini inbred lines BS15 and BS9 as susceptible and resistant parents to prepare F 2 Generational segregation populations, screened with SSR primers for dominant resistance genes related to zucchini powdery mildew Pm1 Molecular markers linked to obtain a co-dominant molecular marker Pm-136, which is associated with the disease resistance gene Pm1 The genetic linkage distance of 1.7 cm.

[0035] The application of the molecular marker in the identification and transfer of powdery mildew resistance gene of zucchini germplasm resources.

[0...

Embodiment 2

[0046] This embodiment specifically describes the dominant disease resistance gene related to zucchini powdery mildew Pm1 A method for obtaining linked SSR molecular markers, comprising the following steps:

[0047] (1) Establishment and phenotypic identification of the F2 generation of zucchini inbred lines BS15 and BS9:

[0048] (1) Hybrid F1 was obtained by crossing zucchini inbred line BS15 (female parent) with BS9 (male parent), and F1 generation population was produced by selfing of F1.

[0049] (2) The single plant of the F2 generation population was planted in a nutrient pot in a greenhouse, covered with an insect-proof net, and inoculated with powdery mildew spores after the cotyledons were fully unfolded, and the disease resistance traits were investigated 15 days after the inoculation. The identification results are shown in Table 1.

[0050]

[0051] R and S represent resistant and susceptible plants, respectively. χ2 0.05, 1 =3.84.

[0052] (3) The tra...

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Abstract

The invention discloses an SSR molecular marker (Pm-136) linked with a powdery mildew resistant gene pm1 of cucurbita pepo L., belongs to the technical field of breeding of vegetable molecules for disease resistance, and relates to an SSR molecular marker linked with the powdery mildew resistant gene, and an application of the SSR molecular marker. The genetic linkage distance between the molecular marker Pm-136 and the resistance gene is 1.7cm. Whether a disease resistance character transfer offspring plant carries with the resistance gene Pm1 or not can be rapidly and accurately detected by virtue of the molecular marker Pm-136, thus the disease-resistant breeding efficiency is significantly improved; and the breeding cycle is shortened. The obtained marker Pm-136 has the advantages of being stable, reliable, simple to operate, good in repeatability and the like, and has high utilization value in identification of resistant and susceptible varieties and a disease-resistant transfer single offspring plant.

Description

technical field [0001] The invention relates to the technical field of vegetable molecular disease-resistant breeding, specifically a kind of Cucurbitapepo L.) Powdery mildew dominant disease resistance gene Pm1 Linked SSR molecular markers and the application of the markers in detecting disease-resistant plants. Background technique [0002] Zucchini is one of the important vegetables of the pumpkin genus that is widely planted around the world. The cultivated area in my country is increasing year by year. Especially in recent years, protected cultivation has developed rapidly, and it has become the second largest cultivated crop after cucumber. Zucchini production is of great significance to increasing farmers' income, adjusting agricultural structure and enriching the people's vegetable basket. [0003] Powdery mildew is one of the important diseases that harm the mid-to-late production of zucchini. After the plant is infected with powdery mildew, photosynthesis is se...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 李海真张国裕张帆贾长才姜立纲
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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