Linked Molecular Marker and Application of the Dominant Resistance Gene pm1 to Powdery Mildew of Cucurbita

A disease-resistant gene and molecular marker technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as no research reports on zucchini, and achieve convenient identification, stable amplification, and accelerated utilization. Effect

Active Publication Date: 2018-01-16
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on molecular markers linked to melon powdery mildew resistance is mainly concentrated on watermelon, melon and cucumber, and there is no relevant research report on zucchini at home and abroad.

Method used

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  • Linked Molecular Marker and Application of the Dominant Resistance Gene pm1 to Powdery Mildew of Cucurbita
  • Linked Molecular Marker and Application of the Dominant Resistance Gene pm1 to Powdery Mildew of Cucurbita
  • Linked Molecular Marker and Application of the Dominant Resistance Gene pm1 to Powdery Mildew of Cucurbita

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Experimental program
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Effect test

Embodiment 1

[0032] Dominant resistance gene to powdery mildew of zucchini Pm1 The linked SSR molecular marker, said molecular marker is Pm-136, which has the DNA sequence described in SEQ ID NO. 1 and SEQ ID NO. 2 in the sequence listing.

[0033] The zucchini powdery mildew dominant disease resistance gene Pm1 A method for obtaining linked SSR molecular markers, comprising the following steps:

[0034] Application of SSR-BSA technology: using zucchini inbred lines BS15 and BS9 as susceptible and resistant parents to prepare F 2 Generational segregation populations, screened with SSR primers for dominant resistance genes related to zucchini powdery mildew Pm1 Molecular markers linked to obtain a co-dominant molecular marker Pm-136, which is associated with the disease resistance gene Pm1 The genetic linkage distance of 1.7 cm.

[0035] The application of the molecular marker in the identification and transfer of powdery mildew resistance gene of zucchini germplasm resources.

[0...

Embodiment 2

[0046] This embodiment specifically describes the dominant disease resistance gene related to zucchini powdery mildew Pm1 A method for obtaining linked SSR molecular markers, comprising the following steps:

[0047] (1) Establishment and phenotypic identification of the F2 generation of zucchini inbred lines BS15 and BS9:

[0048] (1) Hybrid F1 was obtained by crossing zucchini inbred line BS15 (female parent) with BS9 (male parent), and F1 generation population was produced by selfing of F1.

[0049] (2) The single plant of the F2 generation population was planted in a nutrient pot in a greenhouse, covered with an insect-proof net, and inoculated with powdery mildew spores after the cotyledons were fully unfolded, and the disease resistance traits were investigated 15 days after the inoculation. The identification results are shown in Table 1.

[0050]

[0051] R and S represent resistant and susceptible plants, respectively. χ2 0.05, 1 =3.84.

[0052] (3) The traits...

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Abstract

The invention discloses a linked SSR molecular marker (Pm-136) with the dominant disease resistance gene Pm1 of zucchini powdery mildew, belongs to the technical field of molecular disease resistance breeding of vegetables, and relates to the linked SSR molecular marker of the dominant disease resistance gene of powdery mildew and its application . The genetic distance between the molecular marker Pm‑136 and the disease resistance gene was 1.7 cm. The molecular marker Pm-136 can be used to quickly and accurately detect whether the progeny of the disease-resistant traits carry the Pm1 disease-resistant gene, thereby significantly improving the efficiency of disease-resistant breeding and shortening the breeding cycle. The Pm-136 marker obtained by the present invention has the advantages of being stable, reliable, simple to operate, and good in repeatability, and has very high utilization value in identifying resistant and susceptible varieties and disease-resistant transgenic individual plants.

Description

technical field [0001] The invention relates to the technical field of vegetable molecular disease-resistant breeding, specifically a kind of Cucurbitapepo L.) Powdery mildew dominant disease resistance gene Pm1 Linked SSR molecular markers and the application of the markers in detecting disease-resistant plants. Background technique [0002] Zucchini is one of the important vegetables of the pumpkin genus that is widely planted around the world. The cultivated area in my country is increasing year by year. Especially in recent years, protected cultivation has developed rapidly, and it has become the second largest cultivated crop after cucumber. Zucchini production is of great significance to increasing farmers' income, adjusting agricultural structure and enriching the people's vegetable basket. [0003] Powdery mildew is one of the important diseases that harm the mid-to-late production of zucchini. After the plant is infected with powdery mildew, photosynthesis is sev...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 李海真张国裕张帆贾长才姜立纲
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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