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Molecular markers of Chinese squash powdery mildew and primer pairs for identifying resistance traits of Chinese squash powdery mildew

A Chinese pumpkin, molecular marker technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as no Chinese pumpkin research reports

Active Publication Date: 2019-12-31
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on molecular markers linked to melon powdery mildew resistance is mainly concentrated on watermelon, muskmelon and cucumber, and there is no relevant research report on Chinese pumpkin at home and abroad.

Method used

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  • Molecular markers of Chinese squash powdery mildew and primer pairs for identifying resistance traits of Chinese squash powdery mildew
  • Molecular markers of Chinese squash powdery mildew and primer pairs for identifying resistance traits of Chinese squash powdery mildew
  • Molecular markers of Chinese squash powdery mildew and primer pairs for identifying resistance traits of Chinese squash powdery mildew

Examples

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Embodiment 1

[0081] Embodiment 1, identification of Chinese pumpkin powdery mildew resistance traits

[0082]In this example, the primers used to identify or assist in the identification of powdery mildew resistance traits of Chinese squash are used to identify the powdery mildew resistance traits of Chinese squash by A1. A1 is composed of single-stranded DNA named P1 and P2, and P1 is SEQ ID No. The single-stranded DNA shown in the 1-20 position of 1, and P2 is the single-stranded DNA reverse complementary to the 171-191 position of SEQ ID No.1.

[0083] 1. The Chinese pumpkins to be identified are as follows, and 50 plants of each Chinese pumpkin are selected for testing:

[0084] Chinese pumpkin inbred line BCmo27, ​​whose phenotype is susceptible to powdery mildew;

[0085] Chinese pumpkin inbred line BCmo3, whose phenotype is resistant to powdery mildew;

[0086] Shanmei No. 2, whose phenotype is susceptible to powdery mildew, is a product of Shantou Jinhan Seed Industry Co., Ltd.; ...

Embodiment 2

[0108] Example 2. The method for obtaining the SSR molecular marker closely linked to the Chinese pumpkin powdery mildew dominant disease resistance gene CmoPm1

[0109] The Chinese pumpkin powdery mildew molecular marker in Example 1 is named molecular marker Pm103, and this embodiment specifically describes the method for obtaining the SSR molecular marker Pm103 closely linked with the Chinese pumpkin powdery mildew dominant disease resistance gene CmoPm1, including the following step:

[0110] (1) Chinese pumpkin inbred lines BCmo27 and BCmo3F 2 Generation generation and phenotyping:

[0111] (1) The Chinese pumpkin inbred line BCmo27 (female parent, P1) was crossed with BCmo3 (male parent, P2) to obtain the hybrid F 1 , F 1 F 2 generation groups.

[0112] (2)F 2 The single plant of the generation group was planted in a nutrient pot in a greenhouse, covered with an insect-proof net, and inoculated with powdery mildew spores after the cotyledons were fully expanded, an...

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Abstract

The invention discloses a China pumpkin powdery mildew molecular marker and a primer pair identifying the resistance character of China pumpkin powdery mildew. The China pumpkin powdery mildew molecular marker is a DNA molecule with the genomic DNA of the China pumpkin as a template, wherein the DNA molecule is obtained by conducting amplification through the primer pair A1; and A1 is composed of single stranded DNA named P1 and P2, wherein P1 is the single stranded DNA which is in specific binding with the sixty fifth upstream part in SEQ ID No.1, and P2 is the single stranded DNA which is in specific binding with the eighty second downstream part in SEQ ID No.1. It is proved through experiments that the primer pair A1 for conducting identifying or auxiliary identifying on the resistance character of the China pumpkin powdery mildew and the China pumpkin powdery mildew molecular marker can be utilized for identifying the resistance condition of the China pumpkin powdery mildew.

Description

technical field [0001] The invention relates to a molecular marker for Chinese pumpkin powdery mildew and a pair of primers for identifying Chinese pumpkin powdery mildew resistance traits in the field of biotechnology. Background technique [0002] my country is the main country in which pumpkins are cultivated in China, and its cultivation area and output rank among the top in the world. Especially in recent years, the cultivation area of ​​Chinese pumpkins in southern China has continued to increase, and it has become an important cultivated crop for farmers to increase their income and adjust their agricultural structure. [0003] Powdery mildew is one of the important diseases that harm the middle and late production of Chinese pumpkin. After the plant is infected with powdery mildew, the photosynthesis is seriously reduced, the plant grows slowly, and the yield and quality are reduced. The yield loss can reach about 40%. Chinese pumpkin powdery mildew is mainly caused ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张国裕李海真张帆贾长才姜立纲
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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