11 results about "Cucurbita moschata" patented technology

The utility model discloses an airlift type muskmelon outer vesicle culture and separation device which comprises a culture tank, the culture tank is a glass tank body with a big-end-up caliber, the top of the large caliber of the culture tank is connected with an exhaust valve through threads, and one side of the small caliber of the culture tank is fixedly connected with an air filter. One side of the air filter is fixedly connected with an air compressor, the culture tank is in threaded connection with a regulating valve, the output end of the regulating valve is connected with a pressure pump through a connecting pipe, the output end of the pressure pump is connected with an ultrafilter through a connecting pipe, and the bottom of the ultrafilter is provided with a discharge port; the outer surface of the side wall of the culture tank is in threaded connection with a temperature controller and a cooling water inlet, and the outer wall of the other side of the culture tank is in threaded connection with a cooling water outlet. The device is convenient to operate, fresh plant tissues do not need to be crushed traditionally, and the outer vesicles of the cucurbita moschata are cultured and enriched through airlift illumination suspension, so that the time and the labor cost are saved.

The present utility model relates to a food composition, specifically a squash (Cucurbita moschata) tart in which squash (Cucurbita moschata) serves as the principal filling ingredient. Squash (Cucurbita moschata) is underutilized in commercial pastries due to its high moisture content and the perceived difficulty of developing a stable, smooth-textured tart filling. It provides nutritional benefits, being rich in beta-carotene, vitamins, and dietary fiber. The composition comprises puréed squash (Cucurbita moschata), all-purpose flour, milk, cream cheese, white sugar, butter, de-shelled whole egg, cornstarch, yeast, vanilla extract, and salt. The utility model further discloses a preparation method involving steaming the squash (Cucurbita moschata) to enhance sweetness, soften coarse fibers, and retain nutrients prior to puréeing. The resulting squash (Cucurbita moschata) purée is combined with wet and dry ingredients to form a uniform mixture, which is baked to achieve a smooth and cohesive tart structure with consistent quality.

The present invention provides a novel squash plant having an especially small seed cavity, and a method for producing the squash plant. The present invention provides a method for producing a small seed cavity squash plant that includes developing, from the progeny of an F1 seed obtained by crossing a Cucurbita maxima and a Cucurbita moschata, a small seed cavity squash plant in which the proportion of the volume of the seed cavity relative to the fruit volume is 15% or less, and also provides a small seed cavity squash plant that is a small seed cavity Cucurbita maxima plant having, within the nuclear genome, a gene associated with a small seed cavity trait of a plant of the genus Cucurbita, or a hybrid plant obtained using the small seed cavity Cucurbita maxima plant as a parent, or a progeny of either of these plants.

The invention relates to the technical field of agricultural biology, and particularly provides a preparation method and application of a nanocrystallization preparation for preventing and treating Chinese pumpkin leaf curl virus on muskmelons. Based on an RNAi interference technology, an efficient target gene region DNA-A-AV2 for inhibiting virus infection is screened, corresponding dsRNA is induced and synthesized, zinc oxide (ZnO) is used for loading and then is delivered into a plant body, the stability of the dsRNA is improved, long-acting slow release is realized, and the prevention and treatment effect of the dsRNA is enhanced. Experiments show that the nano pesticide can effectively inhibit replication and diffusion of viruses and reduce the content of the viruses. Compared with traditional chemical agents and conventional breeding, the method has irreplaceable advantages in pertinence, safety and adaptability, and meets the requirements of current agriculture for'precision 'and'green'.

The invention belongs to the technical field of plant tissue culture, and discloses a method for cultivating detoxified tissue culture seedlings of trichosanthes kirilowii maxim and the detoxified tissue culture seedlings obtained by the method. The method comprises the following steps: taking a terminal bud or an axillary bud of a trichosanthes kirilowii Maxim plant, performing surface disinfection, and stripping a 0.3-0.5 mm stem tip under a sterile condition to serve as an explant; inoculating the stem tips into a primary culture medium for induction culture, wherein the primary culture medium is an MS culture medium added with 1.0 mg / L 6-BA and 0.2 mg / L NAA; after the adventitious buds germinate, sequentially performing multiplication culture, subculture and rooting culture to obtain complete tissue culture seedlings; and finally carrying out acclimatization and transplantation. According to the method, a systematic and efficient stem tip detoxification culture system is established for trichosanthes kirilowii maxim for the first time, and by optimizing explant selection, disinfection procedures, a key culture medium formula and culture conditions, rapid propagation is achieved, and meanwhile the survival rate of the trichosanthes kirilowii maxim is increased. Various viruses such as cucurbit light mottle mosaic virus (CuMMV) and zucchini green mottle mosaic virus (ZGMMV) carried in a trichosanthes kirilowii Maxim plant can be efficiently removed, and the detoxification rate can reach 96% or above.

The utility model discloses a detecting and sampling device for solid anaerobic fermentation of cucurbita moschata, which comprises a sampling structure, the sampling structure comprises a sampler outer pipe, a conical pipe head connected to the tail end of the sampler outer pipe, an outer pipe sampling opening formed in the outer wall of the sampler outer pipe, and a sampler inner pipe arranged on the inner side of the sampler outer pipe in a sleeving manner; the sampling wrench is connected to the top end of the sampler inner pipe; the inner pipe sampling opening is formed in the outer wall of the sampler inner pipe; the ventilation structure comprises a ventilation pipe arranged in the sampler inner pipe, a connecting port communicated with the top end of the ventilation pipe and an air outlet formed in the surface of the ventilation pipe. According to the utility model, the sampling structure and the ventilation structure are matched with each other, so that air is prevented from entering in the sampling process, the fermentation effect is ensured, and solid anaerobic fermentation samples in different deep layers can be sampled as required.

The invention provides a pumpkin powdery mildew resistance related KASP molecular marker which corresponds to a 2483681th nucleotide mutation site on a pumpkin 19 # chromosome, and the mutation site is T-C mutation; the mutation site is located on a CmoCh19G003220 gene of a No.19 chromosome of the pumpkin. The invention also provides a primer group for detecting the molecular marker. The primer group comprises a forward primer F1, a forward primer F2 and a reverse primer R. Meanwhile, the invention further provides application of the molecular marker or the primer group and a pumpkin powdery mildew resistance identification method. Based on the molecular marker, pumpkin powdery mildew resistance genotype identification, germplasm screening and variety breeding are carried out, the efficiency and accuracy of resistance identification can be remarkably improved, the breeding cost is reduced, rapid excavation of resistance germplasm and directional breeding of disease-resistant varieties are achieved, and efficient molecular technical support is provided for pumpkin powdery mildew resistance breeding.

The utility model provides a sterile sampling device for detecting fermentation components of cucurbita moschata, which comprises a sampling interface, a first liquid stop valve, a first liquid filter, a negative pressure device interface, a sterile injector, an air filter and a three-way pipeline, the first liquid stop valve is arranged outside a connecting pipe, and the sampling interface is arranged at the lower end of the connecting pipe; by means of the design, the problems that according to conventional fermentation tank equipment, when a sampling opening is used every time, steam sterilization is needed for at least 10 minutes, then sample receiving is conducted, a part of fermentation liquor is consumed in each link, sterilization is incomplete, contamination is prone to occurring, use is inconvenient, time and labor are wasted, and cost loss is large are solved. The device can directly carry out moist heat sterilization, reduces the contamination rate of the tank body, is simple to operate, is convenient for carrying out massive sampling work, and reduces the cost caused by steam sterilization and fermentation liquor loss in the fermentation link as sampling is carried out infinitely until fermentation is finished.

The utility model discloses a swing arm type cucurbita moschata dicing head mechanism which comprises a swing arm mechanism, a longitudinal cutting sliding table air cylinder mechanism, a horizontal cutting sliding table air cylinder mechanism and a cucurbita moschata fixing platform, and the longitudinal cutting sliding table air cylinder mechanism and the horizontal cutting sliding table air cylinder mechanism are arranged around the cucurbita moschata fixing platform. The cut-in distance and the cut-in angle of the two longitudinal cutters are adjustable, and the two horizontal cutters are matched with the cut-in angle and the horizontal dicing distance of the two longitudinal cutters; longitudinal dicing actions and horizontal dicing actions are oppositely arranged, so that the longitudinal dicing actions and the horizontal dicing actions can be simultaneously performed without mutual influence, the overall dicing efficiency is improved, the overall structure is simple and independent, the requirement for the manufacturing precision of parts is low, and the assembly manufacturability is good; the cutting-in action of the whole machine adopts an air cylinder to push a blade to cut in, the response speed is high, the operation is simple and convenient, the position where the blade feeding needs to be accurately controlled is controlled by a sliding table lead screw pair, and enough force is provided for dicing while the movement precision is ensured.

The application relates to the field of agricultural biotechnology, and specifically provides a preparation method and application of a nanoized preparation for preventing and treating Chinese squash leaf curl virus on melons. The application is based on RNAi interference technology, screens a high-efficiency target gene region DNA-A-AV2 for inhibiting virus infection, induces synthesis of corresponding dsRNA, and delivers the dsRNA into a plant body after being loaded with zinc oxide (ZnO), so that the stability of the dsRNA is improved, long-acting slow release is realized, and the prevention and treatment effect of the dsRNA is strengthened. Experiments show that the nano pesticide can effectively inhibit virus replication and spread, and reduce the virus content. Compared with traditional chemical agents and conventional breeding, the application has irreplaceable advantages in terms of pertinence, safety and adaptability, and meets the current agricultural "precision" and "green" requirements.

The present invention provides a squash plant having an especially small seed cavity, and a method for producing the squash plant. The present invention provides a method for producing a small seed cavity squash plant that includes developing, from the progeny of an F1 seed obtained by crossing a Cucurbita maxima and a Cucurbita moschata, a small seed cavity squash plant in which the proportion of the volume of the seed cavity relative to the fruit volume is 15% or less, and also provides a small seed cavity squash plant that is a small seed cavity Cucurbita maxima plant having, within the nuclear genome, a gene associated with a small seed cavity trait of a plant of the genus Cucurbita, or a hybrid plant obtained using the small seed cavity Cucurbita maxima plant as a parent, or a progeny of either of these plants.