93 results about "Cucurbita moschata" patented technology

The invention discloses a method for grafting melons on double rootstocks, comprising the following steps: (1) simultaneously sowing the rootstocks cucurbita moschata and cucurbita ficifolia in pairs; (2) sowing the seeds of the melons 5-8 days after sowing the rootstocks; (3) when the diameter of the first mainleaf of the rootstock cucurbita moschata is 4.5-6cm, removing the first mainleaf of the cucurbita moschata, removing the growing point of the cucurbita ficifolia and grafting the rootstocks by inarching, and meanwhile, bevelling the parts of the lower hypocotyl, 1.5-2.5cm from the cotyledon, of the seedlings of the melons and obliquely inserting the bevelled scions of the melons on the parts of the lower hypocotyl, 0.3-0.7cm from the cotyledon, of the cucurbita moschata by the grafting needles; and (4) managing the seedlings and planting the seedlings 2-4 days later. The method realizes simultaneous inarching of the two rootstocks and cuttage grafting of the scions of the melons and the rootstocks, greatly improves the operation efficiency, greatly lowers the consequent management cost and is very applicable to industrial production of the seedlings raised by grafting.

The invention discloses primers and a method for purity identification of hybrid seeds of Chinese pumpkin 'Guangmi NO.1'. The primers are VRISSRPMK8-F:5'-TAATTAAGGCGTGAAATTGG-3' and VRISSRPMK8-R:5'-GAAAAGAAACATAAACAGGGC-3'. The identification method is as follows: 1) extracting a genome DNA (deoxyribonucleic acid) of a pumpkin seedling; 2) carrying out PCR (polymerase chain reaction) amplification by taking the genome DNA of the pumpkin as a template and using the SSR primers VRISSRPMK8; 3) carrying out gel electrophoresis on the amplified product; and 4) analyzing the electrophoresis result, wherein only the single plant simultaneously having parent specific bands is a real hybrid, and the plant lacking of any one band is marked as false hybrid. The primers and the method can be used for effectively distinguishing the hybrid seed of the 'Guangmi NO.1' from the female parent and the male parent, and can identify the purity of the hybrid seeds rapidly and accurately. The method has the advantages of being fast and accurate, low in cost and simple in operation, and the like.

A method for prevention or therapy of pollen allergy, allergic rhinitis, atopic dermatitis, asthma or urticaria by administration of two kinds of crude drugs—seeds of Cucurbita moschata and flowers of Carthamus tinctorius—and at least one crude rug selected from Plantago asiatica, Lonicera japonica, Glycyrrhiza uralensis, Coix lachrymal-jobi var. ma-yuen, Zingiber officinale, Curcuma longa, Curcuma zedoaria and Artemisia argyi to a patient; and a health food for prevention, or improvement, or reduction of these symptoms containing the above substances.

The invention discloses a cucurbita moschata internal reference gene 18 S rRNA gene sequence, a quantitative specific primer is designed on the basis, the cucurbita moschata 18 S rRNA gene can be stably expressed in each growth and development stage of cucurbita moschata and all abiotic stress conditions, and is suitable for using as an internal reference gene in study of cucurbita moschata gene expression. For the first time, the 18 S rRNA gene as the internal reference gene is used for cucurbita moschata gene expression analysis, helps to improve the stability, reliability and repeatability of the cucurbita moschata gene expression analysis, the detection primer disclosed in the invention is specific, greatly improves the test efficiency, and improves the credibility of the test results.

The invention discloses an indel molecular marker closely linked with pumpkin photoperiod insensitivity and application of the indel molecular marker, and belongs to the technical field of molecule detection. The indel molecular marker SEQ7593 is located on a tenth chromosome of a Chinese pumpkin, and is 280bp in size. The nucleotide sequence of the indel molecular marker is shown as SEQ ID NO.1.The indel molecular marker SEQ7593 can be directly used for creating an assistant breeding system of photoperiod insensitivity character molecular markers. Primer amplification designed according to the indel molecular marker can be applied to assistant breeding of pumpkin breed improvement molecules, technical support is provided for pumpkin photoperiod insensitivity molecular breeding, and timefor conventional gene positioning is shortened greatly.

The invention provides a method for producing ferment organic fertilizer by rural wastes. The method comprises the following steps: (1) preparing ferment: mixing brown sugar, raw fruit pulp and distilled water to obtain a mixed solution; putting the mixed solution into a sealed container and storing for 3 months to obtain ferment; (2) mixing the ferment prepared by the step (1), sugar and distilled water according to the ratio and sealing; culturing for 2d at room temperature without light to obtain a ferment strain; (3) uniformly mixing the ferment strain obtained by the step (2) and wheat bran to obtain aerobic fermented solid ferment powder; (4) mixing animal dung with the ferment powder to obtain an aerobic dung strain capable of eliminating odor of the animal dung, having a great decomposition capability and capable of being stored for long time; (5) preparing rural waste organic fertilizer. The organic fertilizer obtained by a preparation method of human urine ferment organic fertilizer has high nutrient value which is equivalent to the level of cucurbita moschata duch produced by Batian tower compound fertilizer; the organic fertilizer has important application value.

The invention discloses a traditional Chinese medicine composition for treating dysmenorrheal. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 4-8 parts of ligusticum wallichii, 3-6 parts of baked ginger, 8-12 parts of rhizoma corydalis, 5-10 parts of trogopterus dung, 10-15 parts of root of common peony, 7-11 parts of solanum melongena, 10-15 parts of fennel, 8-12 parts of pollen typhae, 3-6 parts of cinnamon, 5-8 parts of frankincense, 4-7 parts of myrrh, 9-13 parts of cucurbita moschata duch, 10-15 parts of rhizoma cyperi, 6-12 parts of liquorice, 10-15 parts of motherwort, 5-10 parts of caulis sinomenii, 12-16 parts of sorghum flowers, 4-8 parts of fructus evodiae, 5-10 parts of rhizoma atractylodis, 11-17 parts of durian shell, 14-18 parts of poria cocos, and 10-15 parts of eucommia ulmoides. The traditional Chinese medicine composition disclosed by the invention has the effects of warming channel and expelling cold, promoting blood circulation to arrest pain, regulating qi and blood, nourishing yin and blood and the like, is used for treating the diseases such as irregular menstruation, dysmenorrheal, blood-insufficiency, menstrual dizziness, stomachache, abdominal pain, back pain and the like, and is good in curative effect, fast to take effect, short in treatment course, and free of toxic and side effects; the total effective rate can be up to 97 percent.

The invention relates to a pumpkin breeding method, in particular relates to a cucurbita maxima breeding method, and belongs to the technical field of plant breeding. At present, all of cucurbita maxima have stems; as the stemless cucurbita maxima is dominant for the stemmed cucurbita maxima, all of the first filial generations after the stemless cucurbita maxima is hybridized with the stemmed cucurbita maxima do not have the stems. According to the method, the stemless cucurbita maxima is bred; Chinese pumpkins with high temperature resistance and good disease resistance are utilized as materials; the stemless cucurbita maxima bred through different interspecific hybridization solves the problems that existing cucurbita maxima is not resistant to high temperature and can be easily infected with virus diseases; a new stemless cucurbita maxima variety with good quality and good disease resistance is bred.

The present invention discloses a salt tolerance pumpkin rootstock seed breeding method for cucumber grafting cultivation. The method is characterized by comprising: 1, obtaining a China pumpkin inbred line; 2, obtaining a China pumpkin hybrid; 3, carrying out selection identification on salt tolerance of the China pumpkin hybrid; and 4, carrying out selection identification on field salt tolerance of the grafted cucumber, wherein 1-2 salt tolerance China pumpkin rootstock varieties for cucumber grafting cultivation are finally determined with the steps. The cultivated pumpkin salt tolerance rootstock variety has the following salient characteristics that: the salt tolerance is good; after the bred cucumber rootstock is grafted, the obtained grafted cucumber plant can grow well in the protection ground soil under a condition of salt content of 0.5%, and grow better compared with the grafted cucumber plant grafted with the main rootstock cucurbita ficifolia in the current production; and the yield is increased by 40-50% compared with the yield of the non-grafted cucumber plant, and is increased by 15-20% compared with the yield of the grafted cucumber adopting the cucurbita ficifolia as the rootstock.

The invention provides a breeding method of a vineless Cucurbita moschata Duch and an application thereof, and belongs to the technical field of crop breeding and plant protection. The method comprises the following steps: 1, hybridizing a vineless pumpkin homozygous material A and a long type Chinese pumpkin homozygous material B as parents, so as to obtain F1; 2, using a parent B as the recurrent parent to continuously backcross with the F1 for n times, and continuously optimizing to obtain BCn, continuously backcrossing the BCn for m generations, continuously optimizing to obtain homozygous backcross line material BCnFm+1; 3, hybridizing the BCnFm+1 with the long type Chinese pumpkin homozygous material C to obtain the vineless Cucurbita moschata Duch. The technical problem that Cucurbita moschata Duch is not beneficial to close planting in the prior art is solved. The vineless Cucurbita moschata Duch is early-maturing, in long rod shape, good in taste, beneficial to close planting and high in the yield. According to the difference of the ecological conditions in the north and south regions, the remote continuous optimization is carried out, the regional adaptability is high, and considerable economic benefits can be brought to enterprises and farmers.

The invention belongs to the field of traditional Chinese medicine application, and in particular discloses traditional Chinese medicinal paste for treating flat wart. The traditional Chinese medicinal paste is prepared from safflower, coix seed, lithospermum, liquorice, folium isatidis, isatis root, rhizoma cyperi, common scouring rush herb, cucurbita moschata duch, broom cypress fruit, alum, dictamnus dasycarpus turcz, endothelium corneum gigeriae galli, barley stalk and clerodendron bungei root according to a certain weight proportion. The traditional Chinese medicinal paste has the advantages of simple preparation, good absorption effect, long drug effect duration, remarkable curative effect, no side effect, high safety and low production cost, and is advantageous to reducing the symptom of a patient in time.

The invention provides a planting method capable of improving the commodity quality of Shenqing series cucumbers. A stock is selected from Japanese cucurbita moschata, a scion is selected from Shenqing cucumbers, and then water and fertilizer management and plant adjustment are carried out. The problems that during Shenqing series cucumber production, brightness is insufficient, and wax powder exists are solved, the commodity quality of the cucumbers is improved, the yield is also increased, the income of vegetable growers is greatly increased accordingly, and practical significance is achieved.

The invention discloses a creation method of a pumpkin interspecific crossing recombinant inbred line. The method comprises the steps that winter squash is used a female parent, cucurbita moschata isused as a male parent, F1 hybrid seeds are obtained, then winter squash is used as a recurrent parent, and the interspecific crossing recombinant inbred line is obtained through a backcross and selfing method; the interspecific crossing recombinant inbred line has the performance of progeny reproduction. Along with increasement of the selfing generation number, the progeny fertility is gradually restored, the fertility of high-generation inbred lines of the fourth inbred generation and the fifth inbred generation is basically close to a normal level, and the reproductive performance of the interspecific crossing recombinant inbred line is good. By adopting the method, the problems can be solved that hybridization of cucurbita moschata and winter squash is difficult, and interspecific crossing hybrids and selfed progeny thereof are difficultly obtained.

The invention belongs to the field of food processing, and particularly relates to a nutritious solanumtuberosum and cucurbita moschata snack pack and a preparation method thereof. The snack pack comprises a solanumtuberosum and cucurbita moschata powder pack and a condiment pack, wherein the solanumtuberosum and cucurbita moschata powder pack comprises the following raw materials in parts by weight: 70-75 parts of solanumtuberosum and cucurbita moschata powder, 5-10 parts of cooked soybean flour, 8-10 parts of cooked rice flour, 1.0-2.5 parts of calcium carbonate, 0.5-1.0 part of vitamin A, 0.5-1.0 part of vitamin D, and 0.3-0.5 part of edible iodized salt. The nutritious solanumtuberosum and cucurbita moschata snack pack disclosed by the invention is scientific and balanced in nutrition collocation; the condiment pack and the solanumtuberosum and cucurbita moschata powder pack are packaged separately, solanumtuberosum and cucurbita moschata paste with various taste can be made, and requirements of the flavor and the mouth feel of different consumers can be met; the moisture content is lower than 10%, so that not only is the quality guarantee period of products prolonged, but also the transporting cost and the packaging cost are greatly reduced; and besides, the loss of nutrient substances is effectively avoided, and the erosion of packaging materials is effectively avoided.

The present invention relates to a method for identifying the authenticity of Cucurbita maxima*Cucurbita moschata hybrid rootstock varieties, and belongs to the field of biology. In the prior art, identification of the variety authenticity is increasingly important for the maintaining of the interests of producers and breeders; the Indian pumpkin*Chinese pumpkin hybrid rootstock varieties are more, and are similar in appearance; and the application of the morphological indexes to identify the authenticity has disadvantages of long time consuming and poor reliability. In order to solve the problems in the prior art, the invention provides the method for identifying the authenticity of the variety by using molecular markers. The technical scheme comprises that three rootstock varieties and control materials are subjected to PCT amplification with 5 pairs of SSR primers (SEQ ID NO.1-10), the generated 9 difference sites are assigned with 0 or 1, and a variety fingerprint for variety authenticity identification is constructed. According to the present invention, the Cucurbita maxima*Cucurbita moschata hybrid rootstock varieties such as Yongzhen 7, Yongzhen 8 and Yongzhen 10 are accurately and effectively identified and protected through the sensitive identification method at the low time cost and the low labor cost.

The invention discloses a medicinal preparation for treating deficiency cold of spleen and stomach type peptic ulcer and a preparation method thereof. The medicinal preparation comprises the following crude drugs: brassica oleracea, lepisorus waltonii, rhizoma zingiberis, hyoscyamus, cortex cinnamomi, clarified butter, fructus foeniculi, fructus ulmi macrocarpae, syzygium aromaticum, pepper, schisandra propinqua, fructus piperis longi, dried tangerine or orange peel, notopterygium root, radix aucklandiae, cayratia japonica, santalum album, semen litchi, potentilla chinensis, prunus mume, allium macrostemon, cucurbita moschata duch, herba aristolochiae, calcicola aristolochia root, rhizoma nardostachyos, diospyros kaki receptacle, valeriana officinalis, medicated leaven, cathay quince, rice-grain sprouts, Japanese pollia herb, semen raphani, resina ferulae, areca catechu, desmodium pulchellum, astragalus membranaceus, rhizoma atractylodis macrocephalae, common crapemyrtle leaves, Chinese yam, pyrola rotundifolia, white hyacinth beans, rhodiola rosea and hippophae rhamnoides. The medicinal preparation for treating deficiency cold of spleen and stomach type peptic ulcer has the efficacies of warming the middle-jiao and invigorating the spleen, and regulating stomach to relieve pain, has the major function of treating deficiency cold of spleen and stomach type peptic ulcer, can not only reduce gastric acid secretion, but also promote ulcer healing and protect gastric mucosa, and is short in treatment course, free of toxic and side effect and obvious in curative effect.

The invention relates to a gene matching, double-excellence combination and system circulation breeding method for a dwarf and yellow-stalk Chinese pumpkin, which belongs to the field of breeding methods of agricultural plant new species. male parent and female parent strains, signs, capital and lower-case figures and attributes are combined into eight groups according to a yin and yang matching principle of the book of changes and the odd and even attributes to carry out permutation and combination; eight self-bred lines exactly form four pairs of combinations with relative characteristics and colors and meet an even system of dwarf to sprawl, a yellow-stalk to a green stalk and female and male matching to carry out the operation of hybridized combination; and the bred new species contains general flavone so that the bred species has the functions of dwarf, yellow stalk, high quality, high yield, prematurity, specificity and disease resistance, thereby opening medicament preparation or health food preparation by the pumpkin and creating great economic benefit for society.

The invention discloses a seedling raising method for loofah by grafting. The method comprises the following steps: a, cultivation of rootstock seedlings: a step of selecting cucurbita moschata seeds grown in a northern alpine region, and cultivating the rootstock seedlings until the rootstock seedlings have one leaf and one top bud, or two leaves and one top bud; b, cultivation of scion seedlings: a step of cultivating loofah seedlings, and selecting the loofah seedlings with unbent hypocotyls and two incompletely-applanate cotyledons as the scion seedlings; c, grafting: a step of grafting the scion seedlings obtained in the step b onto the rootstock seedlings obtained in the step a through a terminal-bud cuttage grafting method so as to obtain grafted seedlings; and d, management after grafting: a step of cultivating the grafted seedlings obtained in the step c for 5 to 7 days under the condition of 26 to 30 DEG C so as to obtain survived grafted seedlings. The seedling raising method for the loofah by grafting provided by the invention can realize early cultivation and early marketing of the loofah under the condition of a condition of unchanged climate, so good economic benefits are obtained.

The invention discloses a nano-sized inositol-containing cucurbita moschata duch extract, a preparation method thereof, and an application thereof. The cucurbita moschata duch extract contains 3% to 5% of total inositols, and has an average particle size of 300nm to 500nm. The preparation method comprises steps that: cucurbita moschata duch is extracted by using an extracting agent; inositol derivatives are enzymolyzed by using phytase, such that inositol monomers are released; through ion exchange resin separation, a cucurbita moschata duch inositol extract containing inositol monomers and inositol derivatives is obtained; through a high-energy ball milling method, the extract is prepared into a nano-sized cucurbita moschata duch extract with a proper particle size. As a result of experiments, the nano-sized inositol-containing cucurbita moschata duch extract has a good hypoglycemic auxiliary effect, and can be applied in preparations of diabetes mellitus novel medicines and various healthcare preparations.

The invention discloses a culture method of muskmelons with artificial pollination. The method comprises following steps: utilizing cucurbita moschata stock and Jinmi NO.1 melon stock for grating, planting grated seedlings to nutrient soil in a shed, wherein the nutrient soil comprises a leaf mold layer, a rice-wheat powdery stone layer, a traditional Chinese medicine slag layer with water content of 60%, an organic fertilizer layer and a sea sand layer; pollination management: spraying a pollination agent to melon plants during a flowering phase and utilizing far-red light of 760nm to illuminate. The preparation method of the pollination agent comprises following steps: mixing uniformly melon flower powder with activated carbon in the environment with 70% of humidity and at a temperature range of 22-25 DEG C to obtain a first mixture; putting boracic acid, fishbone dust, olive oil, aquilaria sinensis peel extracts, tea extracts into water and carrying out ultrasonic treatment for 15 min to obtain a first solution; mixing uniformly the first solution with the first mixture at 30 DEG C to obtain the pollination agent. The culture method of muskmelons with artificial pollination is high in rate of fruit setting, robust in roots and high in anti-diseases capacity.

The invention discloses nutritional meal replacement powder. The nutritional meal replacement powder is prepared from the following components in percentage by mass: 50 to 70 percent of protein powder, 15 to 25 percent of juice powder and 15 to 25 percent of radix puerariae powder. The protein powder is prepared from the following raw materials: walnut kernel, hazelnut kernel, pine nut, sesamum indicum, kernel of cucurbita moschata duch, fructus anacardium occidentalie linn, apricot kernel, kernel of fructus carya illinoensis (Wangh.) Koch and kernel of fructus macadamia ternifolia F.Muell. The juice powder is prepared from the following raw materials: cherry tomato, grape, kiwi fruit pulp, hami melon pulp, banana pulp and passion fruit pulp. The nutritional meal replacement powder disclosed by the invention can be used for simultaneously supplementing various protein, vitamins, minerals, starch and a small amount of grease which are needed by human bodies and is suitable for vegetarianism and weight-losing people thereof.

The invention relates to goat feed for preventing and controlling infective pleuropneumonia. The goat feed is prepared from, by weight, 60-80 parts of green coarse material, 40-50 parts of concentrated feed and 10-15 parts of Chinese traditional Chinese additive, wherein the Chinese traditional Chinese additive is prepared from 2-3 parts of oroxylum indicum, 2-3 parts of pink reineckea herb, 2-3 parts of Chinese pholidota herb, 2-3 parts of common Christia herb and 2-3 parts of cucurbita moschata(Duch.)poiret. The goat feed can effectively prevent and control infective pleuropneumonia of goats, and the drug resistance caused by antibiotic abuse is avoided.

The invention relates to oral medicine for treating primary dysmenorrheal and a preparation method of the oral medicine. The medicine comprises the following ingredients in parts by mass: 1 to 3 parts of cucurbita moschata duch, 1 to 3 parts of safflowers and 1 to 3 parts of brown sugar. The preparation method of the medicine comprises the following steps of selecting and buying high-quality and clear first-grade cucurbita moschata duch, safflowers and brown sugar according to the mass part ratio; respectively removing impurities and dust from ingredients except the brown sugar for use; firstly soaking the cucurbita moschata duch and the safflowers into cold boiled water to be soaked for about half an hour until the medicine tissues are permeated; then putting the soaked medicine and clarified liquid for soaking the medicine into an earthen pot; injecting the cold boiled water being 35mm exceeding the medicine; performing primary decoration and boiling for 40 minutes; filtering medicine liquid; injecting slight warm boiled water being 30 mm exceeding the medicine; performing secondary decoration and boiling for 35 minutes; performing mixing and concentration to obtain 1080ml of 50-percent traditional Chinese medicine liquid; then adding the brown sugar for use.

The invention discloses a traditional Chinese medicine preparation for preventing abortion of a sow. The traditional Chinese medicine preparation is characterized by being prepared from the following raw materials in parts by weight: 3-8 parts of coix seeds, 1-2 parts of cucurbita moschata duch, 1-3 parts of perilla stem, 3-10 parts of semen cuscutae, 2-6 parts of codonopsis pilosula, 1-5 parts of eucommia ulmoides, 3-8 parts of scutellaria baicalensis, 1-5 parts of cirsium japonicum, 1-2 parts of hawthorn, 1-2 parts of mint, 3-6 parts of platycodon grandiflorum, 2-8 parts of lemons and 2-5 parts of rhizoma cyperi. The traditional Chinese medicine preparation does not contain any antibiotic and chemical synthetic drugs, is simple to prepare, good in absorption effect, remarkable in therapy effect, free of side effects, high in safety and free of biological toxicity, and has a function of remarkably reducing the abortion of the sow; the traditional Chinese medicines are powder.

The invention relates to the technical field of livestock and poultry feeding, and particularly discloses livestock and poultry formula feed based on an optimal plant chemical composition. The livestock and poultry formula feed comprises the following ingredients in parts by weight: 55-68% of basic feed, 20-35% of silage and 5-12% of feed additive, wherein the silage is mixed silage obtained by carrying out mixed fermentation on Daucus carota var. sativa Hoffm. leaves, solanum lycopersicum slags and Cucurbita moschata (Duch. ex Lam.) Duch. ex Poiret peel and pulp; the feed additive comprises the following ingredients in parts by weight: 3-6 parts of Capsicum annuum L. powders, 1-3 parts of fructus perillae, 3-5 parts of Citrus reticulata Blanco peel, 0.3-0.5 part of mannan oligosaccharide,0.5-1 part of sodium butyrate, 0.5-1 part of zinc methionine chelate and 0.05-0.1 part of complex enzyme preparation. A silage combination feed additive obtained by fermenting the Daucus carota var.sativa Hoffm. leaves, the solanum lycopersicum slags and the Cucurbita moschata (Duch. ex Lam.) Duch. ex Poiret peel and pulp is mixed with basic feed to obtain the formula feed, the formula feed is rich in various plant chemical substances, including carotene, vitamins, folic acid, flavone, capsorubin, Perilla frutescens (L.) Britt. essential oil and Citrus reticulata Blanco peel essential oil and absorbable mineral substances, including zinc methionine and the like, nutrient ingredients are balanced so as to meet the nutrient requirements of chicken, the immunity of chicken can be enhanced,and the egg laying performance and the chicken and the quality of eggs can be improved.

The invention discloses a method for producing seeds of a yellow-stem dwarf cucurbita moschata through distant hybridization, belonging to the field of a method for breeding new varieties of plants. Inbred lines of the bred dwarf cucurbita moschata are hybridized with bred yellow-stem vining cucurbita moschata to produce a hybrid F1, and the hybrid F1 is then inbred and purified to produce purebred inbred lines of the yellow-stem dwarf cucurbita moschata which is hybridized with a matched cucurbita maxima to produce a distant hybridization product variety. The method has the advantages that the total flavonoid content, practical value, yield, land utilization rate and economic benefits of the cucurbita moschata are all improved.