Preparation method and application of nano-formulation for preventing and treating cucumber mosaic virus on melon

By using nano-formulations targeting the SLCCNV genome sequence and combining ZnO nanocarriers with dsRNA, the stability and cost issues in SLCCNV control were resolved, achieving efficient and green control of melons.

CN121065190BActive Publication Date: 2026-02-03HAINAN UNIVERSITY SANYA NANFAN RESEARCH INSTITUTE
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Patent Information

Application Number
CN202511627543.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-11-07
Publication Date
2026-02-03
Estimated Expiration
2045-11-07

AI Technical Summary

Technical Problem

Existing technologies for controlling Chinese pumpkin leaf curl virus (SLCCNV) suffer from several drawbacks, including susceptibility to external interference, high labor costs, severe chemical pesticide contamination, and poor stability and short-term effectiveness of RNAi technology in natural environments.

Method used

A nano-formulation specifically targeting the SLCCNV genome sequence was developed. The target gene fragment DNA-A-AV2 was transcribed into dsRNA and combined with zinc oxide (ZnO) nanocarriers. The dsRNA was then applied to melon leaves and its stability and long-term sustained release were achieved through endocytosis, thereby enhancing the plant's disease resistance.

Benefits of technology

It achieves precise and efficient control of SLCCNV, enhances the disease resistance of melons, and the use of nanocarriers avoids pollution from chemical pesticides, is compatible with existing cultivation processes, and reduces costs.

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Abstract

The application relates to the field of agricultural biotechnology, and specifically provides a preparation method and application of a nanoized preparation for preventing and treating Chinese squash leaf curl virus on melons. The application is based on RNAi interference technology, screens a high-efficiency target gene region DNA-A-AV2 for inhibiting virus infection, induces synthesis of corresponding dsRNA, and delivers the dsRNA into a plant body after being loaded with zinc oxide (ZnO), so that the stability of the dsRNA is improved, long-acting slow release is realized, and the prevention and treatment effect of the dsRNA is strengthened. Experiments show that the nano pesticide can effectively inhibit virus replication and spread, and reduce the virus content. Compared with traditional chemical agents and conventional breeding, the application has irreplaceable advantages in terms of pertinence, safety and adaptability, and meets the current agricultural "precision" and "green" requirements.
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