Microbial agent for improving yield and quality of strawberries as well as preparation method and application of microbial agent
By combining Artemisia pyrifolia, Priestella linearis, and Bacillus coagulans as microbial agents, the problem of synergistic disease control and growth promotion in strawberry cultivation has been solved, resulting in improved strawberry yield and quality, which meets the needs of green agriculture.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HUBEI MAOSHENG BIOLOGY CO LTD
- Filing Date
- 2026-01-30
- Publication Date
- 2026-05-12
AI Technical Summary
In current strawberry cultivation, microbial agents have limited functions, making it difficult to achieve a synergistic effect of integrated disease control and growth promotion and quality improvement. This leads to a decline in strawberry yield and quality, and the use of chemical fertilizers causes soil compaction and excessive pesticide residues, affecting economic benefits and health.
A microbial agent composed of Artemisia pyrenes, Priestella linearis, and Bacillus coagulans is used to synergistically enhance the growth vigor and disease control effect of strawberries through hormone secretion, nutrient activation, and antibacterial effects.
It significantly improves strawberry yield and fruit quality, reduces rot rate, enhances strawberry growth and disease resistance, and achieves synergistic effects in disease prevention and growth promotion, which is in line with the concept of green agriculture development.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of microbial technology, specifically relating to a microbial agent for improving strawberry yield and quality, its preparation method, and its application. Background Technology
[0002] Strawberries originated in South America and belong to the Rosaceae family of herbaceous plants. They are also known as pineapple strawberries, foreign strawberries, and ground strawberries. Strawberries have delicious flesh and a good taste. They are rich in nutrients and physiologically active ingredients and are known as the "Queen of Fruits".
[0003] In recent years, the strawberry industry has developed rapidly, with cultivation mainly relying on protected cultivation methods such as solar greenhouses, multi-layer film covering, winter-warm greenhouses, and arched sheds. To increase strawberry yields, many strawberry growers have been increasing the application of chemical fertilizers year after year. Coupled with continuous crop rotation, this has led to soil compaction, increased diseases, and a decline in strawberry yield and quality. The main manifestations include smaller fruit size, more deformed fruit, decreased fruit uniformity, coarser flesh, a more acidic or tasteless flavor, shorter shelf life, and a higher rate of rotten fruit. These issues negatively impact the economic benefits of strawberries and hinder the healthy development of the strawberry industry.
[0004] Furthermore, traditional farming methods rely heavily on chemical pesticides and fertilizers, which can lead to excessive pesticide residues, harming human health and contradicting the concept of green agriculture. Against this backdrop, microbial inoculants, due to their environmentally friendly, efficient, and sustainable characteristics, have become an ideal alternative to chemical inputs and for optimizing strawberry cultivation methods, and have gained widespread attention in the field of crop disease prevention and growth promotion.
[0005] Currently, most microbial agents used for strawberry cultivation are based on single strains, with limited functions, making it difficult to achieve synergistic effects of integrated disease control and growth promotion. While some compound agents attempt to combine different strains, insufficient compatibility between strains prevents the formation of a complementary and synergistic mechanism, resulting in limitations such as limited disease control, unstable growth promotion effects, and low nutrient activation efficiency. Furthermore, they fail to effectively improve fruit storage resistance, thus failing to meet farmers' actual needs for high-yield, high-quality, and highly resilient strawberry cultivation.
[0006] Therefore, developing a compound microbial agent that can achieve integrated disease control, efficient nutrient activation, and synergistic effects in promoting growth and improving quality, while being adapted to the growth characteristics of strawberries, is of great practical significance for promoting the green and sustainable development of the strawberry industry and improving planting efficiency, in response to the core pain points of strawberry cultivation. Summary of the Invention
[0007] The purpose of this invention is to provide a microbial agent that can improve strawberry yield and quality, effectively increase strawberry yield and quality, and improve strawberry storability.
[0008] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows: A microbial agent for improving strawberry yield and quality comprises the following raw materials in parts by weight: 40-50 parts compound microbial agent, 0.3-0.5 parts xanthan gum, 1.5-2 parts glycerol, 4-5 parts citrate-sodium citrate buffer, 1-1.5 parts potassium humate, 0.3-0.5 parts potassium dihydrogen phosphate, 0.2-0.3 parts chelated calcium magnesium, and water to make up to 100 parts.
[0009] Furthermore, the compound microbial agent includes *Arthrobacter xinnanensis*, *Priestella linearis*, and *Bacillus coagulans*.
[0010] Furthermore, the compound microbial agent includes *Arthrobacter xinnanensis* purchased from the China General Microbiological Culture Collection Center (CGMCC), strain accession number CGMCC 1.8987, original accession date March 3, 2009; *Priscilla filamentosa* purchased from the China General Microbiological Culture Collection Center (CGMCC), strain accession number CGMCC 1.8801, original accession date October 21, 2008; and *Bacillus coagulans* purchased from the China General Microbiological Culture Collection Center (CGMCC), strain accession number CGMCC 1.10823, original accession date March 3, 2010.
[0011] Furthermore, the preparation method of the citrate-sodium citrate buffer solution is as follows: Prepare 0.1 mol / L citric acid solution and 0.1 mol / L sodium citrate solution using deionized water. Mix the citric acid solution and sodium citrate solution at a volume ratio of 1:4 until homogeneous. Adjust the pH to 6.5. Autoclave the prepared mixed solution at 121℃ for 20 min and then cool it for later use.
[0012] Furthermore, the preparation method of the compound microbial agent is as follows: (1) First, activate *Priestella linearis* in nutrient broth agar medium, pick a single colony and inoculate it into nutrient broth liquid medium, and incubate at 30℃ and 170 r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture at a rate of 5% into the nutrient gravy liquid medium in the fermenter, and incubate at 30℃ and 170 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL was added, and the fermentation broth of Priestella lineari was obtained. (2) Activate Arthrobacter xinnanensis in LB solid medium, pick a single colony and inoculate it into LB liquid medium, and culture at 20℃ and 210 r / min until OD. 600=0.6 to obtain seed culture, and inoculate the seed culture into LB liquid medium in the fermenter at a rate of 5% and incubate at 20℃ and 210 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL, and the fermentation broth of Arthrobacter xinnanensis was obtained. (3) Activate Bacillus coagulans in MRS medium, pick a single colony and inoculate it into MRS liquid medium, and culture at 30℃ and 200r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture at a rate of 1% into the MRS liquid medium in the fermenter and incubate at 30℃ and 200 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL, and the fermentation broth of Bacillus coagulans was obtained. (4) The prepared Priestella lineari fermentation broth, Arthrobacter xinnani fermentation broth and Bacillus coagulans fermentation broth are mixed in a volume ratio of 3:4:3 to obtain a compound microbial agent.
[0013] A method for preparing a microbial agent to improve strawberry yield and quality includes the following steps: First, prepare a compound microbial agent and a citric acid-sodium citrate buffer solution. Place prepared water in a stirring container, and add xanthan gum, glycerol, and citric acid-sodium citrate buffer solution in sequence. Turn on the stirring device and stir until all components are completely dissolved. Continue to add potassium humate, potassium dihydrogen phosphate, and chelated calcium magnesium to the container, and keep stirring until all solid components are completely dispersed and dissolved. Finally, add the prepared compound microbial agent, stir evenly, and the microbial agent to improve strawberry yield and quality is obtained.
[0014] The present invention also provides the application of the microbial agent for improving strawberry yield and quality, characterized in that the microbial agent significantly improves strawberry yield and quality by preventing diseases and promoting growth.
[0015] Furthermore, it effectively prevents and controls strawberry root rot, strawberry anthracnose, strawberry gray mold, and strawberry wilt.
[0016] This invention uses three functional bacterial strains, namely Arthrobacter xinnanensis, Bacillus coagulans, and Priestella linearis, to form a microbial inoculum.
[0017] Arthrobacter xinnanensis has a strong ability to solubilize phosphorus and potassium, which can convert insoluble phosphorus and potassium in the soil into readily available nutrients that strawberries can absorb. The cell division produced by the metabolism of Arthrobacter xinnanensis can delay the senescence of strawberry leaves, improve photosynthetic efficiency, promote the translocation of photosynthetic products to fruits, and increase the sugar content and weight of individual fruits. It can also secrete antibacterial substances, which have a strong inhibitory effect on pathogens such as gray mold, anthracnose, and wilt.
[0018] Bacillus coagulans can secrete a variety of extracellular enzymes such as phytase and protease, which decompose organic nitrogen and organic phosphorus in the soil. It can convert insoluble phosphorus in the soil into readily available nutrients that strawberries can absorb. Bacillus coagulans can quickly colonize the strawberry rhizosphere and form a biofilm, taking over the living space around the roots and inhibiting the colonization and reproduction of pathogens, thus effectively preventing gray mold and root rot in strawberries.
[0019] *Priestella filamentosa* synthesizes auxin IAA and gibberellin, promoting root cell division and elongation in strawberries and increasing root absorption area. It also produces antibacterial substances that directly inhibit strawberry root rot and wilt diseases, and stimulate the plant's own defense system, enhancing its resistance to diseases. Simultaneously, *Priestella filamentosa* can produce siderophores.
[0020] While the function of a single strain is limited, the combination of the above three strains can achieve complementary and synergistic effects: the rapid colonization of Bacillus coagulans provides a stable rhizosphere environment for Priestella linearis and Arthrobacter xinnanensis; the nutrients activated by Arthrobacter xinnanensis can promote the secretion of growth regulators by Priestella linearis, further enhancing the growth-promoting effect; the synergistic effect of the metabolites of the three strains has a significantly better inhibitory effect on pathogens than a single strain, while also improving the growth vigor of strawberries.
[0021] Beneficial effects The microbial agent of this invention can effectively prevent and control strawberry diseases, including root rot, wilt, gray mold, and anthracnose, with the best effect on gray mold.
[0022] The microbial agent of this invention promotes the root development and nutrient absorption of strawberry plants and promotes strawberry growth through the synergistic effects of hormone secretion, nutrient activation and disease inhibition. At the same time, it significantly increases strawberry yield and fruit quality, and significantly reduces the rate of strawberry rot during storage. Detailed Implementation
[0023] The technical solution of the present invention will be further described below with reference to specific embodiments, but it is not limited thereto.
[0024] Example 1 A microbial agent for improving strawberry yield and quality comprises the following raw materials in parts by weight: 40 parts compound microbial agent, 0.3 parts xanthan gum, 1.5 parts glycerol, 4 parts citrate-sodium citrate buffer, 1 part potassium humate, 0.3 parts potassium dihydrogen phosphate, 0.2 parts chelated calcium magnesium, and water to make up to 100 parts.
[0025] The compound microbial agent includes Arthrobacter xinnanensis, Priestella linearis, and Bacillus coagulans.
[0026] The compound microbial agent includes *Arthrobacter xinnanensis* strain with accession number CGMCC 1.8987; *Priscilla filamentosa* strain with accession number CGMCC 1.8801; and *Bacillus coagulans* strain with accession number CGMCC 1.10823.
[0027] The method for preparing the citric acid-sodium citrate buffer solution is as follows: Prepare 0.1 mol / L citric acid solution and 0.1 mol / L sodium citrate solution using deionized water. Mix the citric acid solution and sodium citrate solution at a volume ratio of 1:4 until homogeneous. Adjust the pH to 6.5. Autoclave the prepared mixed solution at 121℃ for 20 min and then cool it for later use.
[0028] The preparation method of the composite microbial agent is as follows: (1) First, activate *Priestella linearis* in nutrient broth agar medium, pick a single colony and inoculate it into nutrient broth liquid medium, and incubate at 30℃ and 170 r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture at a rate of 5% into the nutrient gravy liquid medium in the fermenter, and incubate at 30℃ and 170 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL was added, and the fermentation broth of Priestella lineari was obtained. (2) Activate Arthrobacter xinnanensis in LB solid medium, pick a single colony and inoculate it into LB liquid medium, and culture at 20℃ and 210 r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture into LB liquid medium in the fermenter at a rate of 5% and incubate at 20℃ and 210 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL, and the fermentation broth of Arthrobacter xinnanensis was obtained. (3) Activate Bacillus coagulans in MRS medium, pick a single colony and inoculate it into MRS liquid medium, and culture at 30℃ and 200r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture at a rate of 1% into the MRS liquid medium in the fermenter and incubate at 30℃ and 200 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL, and the fermentation broth of Bacillus coagulans was obtained. (4) The prepared Priestella lineari fermentation broth, Arthrobacter xinnani fermentation broth and Bacillus coagulans fermentation broth are mixed in a volume ratio of 3:4:3 to obtain a compound microbial agent.
[0029] A method for preparing a microbial agent to improve strawberry yield and quality includes the following steps: First, prepare a compound microbial agent and a citric acid-sodium citrate buffer solution. Place prepared water in a stirring container, and add xanthan gum, glycerol, and citric acid-sodium citrate buffer solution in sequence. Turn on the stirring device and stir until all components are completely dissolved. Continue to add potassium humate, potassium dihydrogen phosphate, and chelated calcium magnesium to the container, and keep stirring until all solid components are completely dispersed and dissolved. Finally, add the prepared compound microbial agent, stir evenly, and the microbial agent to improve strawberry yield and quality is obtained.
[0030] Example 2 A microbial agent for improving strawberry yield and quality comprises the following raw materials in parts by weight: 45 parts compound microbial agent, 0.4 parts xanthan gum, 1.7 parts glycerol, 4.5 parts citrate-sodium citrate buffer, 1 part potassium humate, 0.4 parts potassium dihydrogen phosphate, 0.3 parts chelated calcium magnesium, and water to make up to 100 parts.
[0031] The compound microbial agent includes Arthrobacter xinnanensis, Priestella linearis, and Bacillus coagulans.
[0032] The compound microbial agent includes *Arthrobacter xinnanensis* strain with accession number CGMCC 1.8987; *Priscilla filamentosa* strain with accession number CGMCC 1.8801; and *Bacillus coagulans* strain with accession number CGMCC 1.10823.
[0033] The method for preparing the citric acid-sodium citrate buffer solution is as follows: Prepare 0.1 mol / L citric acid solution and 0.1 mol / L sodium citrate solution using deionized water. Mix the citric acid solution and sodium citrate solution at a volume ratio of 1:4 until homogeneous. Adjust the pH to 6.5. Autoclave the prepared mixed solution at 121℃ for 20 min and then cool it for later use.
[0034] The preparation method of the composite microbial agent is as follows: (1) First, activate *Priestella linearis* in nutrient broth agar medium, pick a single colony and inoculate it into nutrient broth liquid medium, and incubate at 30℃ and 170 r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture at a rate of 5% into the nutrient gravy liquid medium in the fermenter, and incubate at 30℃ and 170 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL was added, and the fermentation broth of Priestella lineari was obtained. (2) Activate Arthrobacter xinnanensis in LB solid medium, pick a single colony and inoculate it into LB liquid medium, and culture at 20℃ and 210 r / min until OD. 600=0.6 to obtain seed culture, and inoculate the seed culture into LB liquid medium in the fermenter at a rate of 5% and incubate at 20℃ and 210 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL, and the fermentation broth of Arthrobacter xinnanensis was obtained. (3) Activate Bacillus coagulans in MRS medium, pick a single colony and inoculate it into MRS liquid medium, and culture at 30℃ and 200r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture at a rate of 1% into the MRS liquid medium in the fermenter and incubate at 30℃ and 200 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL, and the fermentation broth of Bacillus coagulans was obtained. (4) The prepared Priestella lineari fermentation broth, Arthrobacter xinnani fermentation broth and Bacillus coagulans fermentation broth are mixed in a volume ratio of 3:4:3 to obtain a compound microbial agent.
[0035] A method for preparing a microbial agent to improve strawberry yield and quality includes the following steps: First, prepare a compound microbial agent and a citric acid-sodium citrate buffer solution. Place prepared water in a stirring container, and add xanthan gum, glycerol, and citric acid-sodium citrate buffer solution in sequence. Turn on the stirring device and stir until all components are completely dissolved. Continue to add potassium humate, potassium dihydrogen phosphate, and chelated calcium magnesium to the container, and keep stirring until all solid components are completely dispersed and dissolved. Finally, add the prepared compound microbial agent, stir evenly, and the microbial agent to improve strawberry yield and quality is obtained.
[0036] Example 3 A microbial agent for improving strawberry yield and quality comprises the following raw materials in parts by weight: 50 parts compound microbial agent, 0.5 parts xanthan gum, 2 parts glycerol, 5 parts citrate-sodium citrate buffer, 1.5 parts potassium humate, 0.5 parts potassium dihydrogen phosphate, 0.3 parts chelated calcium magnesium, and water to make up to 100 parts.
[0037] The compound microbial agent includes Arthrobacter xinnanensis, Priestella linearis, and Bacillus coagulans.
[0038] The compound microbial agent includes *Arthrobacter xinnanensis* strain with accession number CGMCC 1.8987; *Priscilla filamentosa* strain with accession number CGMCC 1.8801; and *Bacillus coagulans* strain with accession number CGMCC 1.10823.
[0039] The method for preparing the citric acid-sodium citrate buffer solution is as follows: Prepare 0.1 mol / L citric acid solution and 0.1 mol / L sodium citrate solution using deionized water. Mix the citric acid solution and sodium citrate solution at a volume ratio of 1:4 until homogeneous. Adjust the pH to 6.5. Autoclave the prepared mixed solution at 121℃ for 20 min and then cool it for later use.
[0040] The preparation method of the composite microbial agent is as follows: (1) First, activate *Priestella linearis* in nutrient broth agar medium, pick a single colony and inoculate it into nutrient broth liquid medium, and incubate at 30℃ and 170 r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture at a rate of 5% into the nutrient gravy liquid medium in the fermenter, and incubate at 30℃ and 170 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL was added, and the fermentation broth of Priestella lineari was obtained. (2) Activate Arthrobacter xinnanensis in LB solid medium, pick a single colony and inoculate it into LB liquid medium, and culture at 20℃ and 210 r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture into LB liquid medium in the fermenter at a rate of 5% and incubate at 20℃ and 210 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL, and the fermentation broth of Arthrobacter xinnanensis was obtained. (3) Activate Bacillus coagulans in MRS medium, pick a single colony and inoculate it into MRS liquid medium, and culture at 30℃ and 200r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture at a rate of 1% into the MRS liquid medium in the fermenter and incubate at 30℃ and 200 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL, and the fermentation broth of Bacillus coagulans was obtained. (4) The prepared Priestella lineari fermentation broth, Arthrobacter xinnani fermentation broth and Bacillus coagulans fermentation broth are mixed in a volume ratio of 3:4:3 to obtain a compound microbial agent.
[0041] A method for preparing a microbial agent to improve strawberry yield and quality includes the following steps: First, prepare a compound microbial agent and a citric acid-sodium citrate buffer solution. Place prepared water in a stirring container, and add xanthan gum, glycerol, and citric acid-sodium citrate buffer solution in sequence. Turn on the stirring device and stir until all components are completely dissolved. Continue to add potassium humate, potassium dihydrogen phosphate, and chelated calcium magnesium to the container, and keep stirring until all solid components are completely dispersed and dissolved. Finally, add the prepared compound microbial agent, stir evenly, and the microbial agent to improve strawberry yield and quality is obtained.
[0042] Comparative Example 1 Compared with Example 3, this comparative example is identical to Example 3 except that the fermentation broths of Priestella lineari, Arthrobacter xinnanensis, and Bacillus coagulans in the compound microbial agent are mixed in a volume ratio of 1:1:1.
[0043] Comparative Example 2 Compared with Example 3, this comparative example is identical to Example 3 except that the compound microbial agent is prepared by mixing Priestella lineari fermentation broth and Arthrobacter xinnanensis fermentation broth at a volume ratio of 3:4.
[0044] Comparative Example 3 Compared with Example 3, this comparative example is identical to Example 3 except that the fermentation broth of Priestella lineari and the fermentation broth of Bacillus coagulans in the compound microbial agent are mixed at a volume ratio of 1:1.
[0045] Comparative Example 4 Compared with Example 3, this comparative example is identical to Example 3 except that the fermentation broth of Arthrobacter xinnanensis and the fermentation broth of Bacillus coagulans in the compound microbial agent are mixed in a volume ratio of 4:3.
[0046] Comparative Example 5 Compared with Example 3, this comparative example uses only Priestella filamentosa fermentation broth in the compound microbial agent, while the other raw materials and steps are the same as in Example 3.
[0047] Comparative Example 6 Compared with Example 3, this comparative example uses only the fermentation broth of Arthrobacter xinnanensis in the compound microbial agent, while the other raw materials and steps are the same as in Example 3.
[0048] Comparative Example 7 Compared with Example 3, this comparative example uses only Bacillus coagulans fermentation broth in the compound microbial agent, while the other raw materials and steps are the same as in Example 3.
[0049] Performance testing Verification of the antibacterial effect of the compound microbial inoculum: Pathogen inhibition test: The plate confrontation method was used. Pathogens of strawberry wilt (Fusarium oxysporum), anthracnose (Colletotrichum gloeosporioides), gray mold (Botrytis cinerea), and root rot (Polytrichum neodymitis) were activated and cultured on PDA plates. 5 mm mycelial cakes were collected and placed in the center of a new PDA plate. Two sterile filter paper discs were placed approximately 2.5 cm from the center of the plate. 10 μL of the compound microbial suspension from Example 3 was added to each filter paper disc. LB broth was used as a blank control. Each treatment was repeated three times and incubated at 25°C for three days. The diameter of the fungal colonies was measured, and the average value was used to calculate the inhibition rate. The data are shown in Table 1.
[0050] Table 1 Inhibitory effect of microbial inoculum
[0051] As shown in the table above, the microbial inoculum formulated in this invention has a good antagonistic effect on strawberry wilt, anthracnose, gray mold and root rot, among which the inhibitory effect on strawberry gray mold is the best.
[0052] Planting Trial The strawberry variety used in the test was Senyan 99. The physical and chemical properties of the soil in the test site were as follows: organic matter 27.64 g / kg, total nitrogen 1.39 g / kg, available phosphorus 37.22 mg / kg, available potassium 184.53 mg / kg, and pH 5.86.
[0053] The experiment consisted of 11 treatment groups: each group was treated with the microbial inoculants prepared in Examples 1-3 and Comparative Examples 1-7, respectively, and a blank control group (water) was included. The amount of microbial inoculant used was 5 L / hm². 2 Dilute 100 times and spray or drench the roots. The blank control group is a water control. The spraying rate is 500 L / hm. 2 The strawberry plants were treated with a root drenching once after planting and a spraying once at the initial fruiting stage. Each treatment was repeated three times, with each plot measuring 30m². 2 High-ridge mulching cultivation, with a planting density of 90,000 plants / hm². 2 .
[0054] Strawberry yield: Twenty strawberry plants were selected from each plot and marked. They were harvested and weighed regularly to calculate the strawberry yield per unit area. At the same time, 20 early-ripening strawberries from each plot were selected and their fruit weight was measured. Strawberry quality: Ten representative strawberries were selected from each plot. The soluble solids content of the fruit was determined by the Abbe refractometer method, the soluble sugar content of the fruit was determined by the anthrone colorimetric method, the vitamin C content of the fruit was determined by the 2,6-dichlorophenolindophenol colorimetric method, and the titratable acid content of the fruit was determined by the acid-base titration method. Strawberry storage: 20 strawberries were selected from each plot. After 5 days of storage, the fruit firmness was measured using a GY-4 fruit firmness tester. After 5 days of storage, the rot was measured and the rot rate was calculated. The above data are shown in Tables 2 and 3.
[0055] Table 2. Effects of different microbial inoculants on strawberry yield and quality.
[0056] As shown in Table 2, the microbial inoculants of Examples 1-3 of this invention can effectively increase the single fruit weight and yield of strawberries, and significantly improve the quality of strawberries, specifically by increasing the content of soluble solids, soluble sugars, and vitamin C in the strawberry fruit. In contrast, comparative examples 2-7, which changed the composition of the compound microbial inoculant, showed varying degrees of decrease in strawberry fruit weight, yield, and quality compared to Example 3, indicating that the effectiveness of the compound microbial inoculants is weakened if any of the microbial components are missing.
[0057] Table 3. Effects of microbial inoculants on the storage quality of strawberries in each treatment group.
[0058] As shown in Table 3, compared to the control (CK), the microbial inoculants of Examples 1-3 of this invention can effectively improve the storability of strawberries, with the rot rate decreasing from 35% to a minimum of 16.7%, and the firmness decreasing from 0.38 kg / cm². 2 Increased to 0.61 kg / cm 2 It is evident that the use of the microbial agent of this invention can significantly improve the storability of strawberries.
[0059] It should be noted that the above embodiments are merely some preferred embodiments of the present invention, and not all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.
Claims
1. A microbial inoculant for improving strawberry yield and quality, characterized in that, The ingredients include the following parts by weight: 40-50 parts of compound microbial agent, 0.3-0.5 parts of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of citrate-sodium citrate buffer, 1-1.5 parts of potassium humate, 0.3-0.5 parts of potassium dihydrogen phosphate, 0.2-0.3 parts of chelated calcium and magnesium, and water to make up to 100 parts.
2. The microbial agent for improving strawberry yield and quality according to claim 1, characterized in that, The compound microbial agent includes Arthrobacter xinnanensis, Priestella linearis, and Bacillus coagulans.
3. The microbial agent for improving strawberry yield and quality according to claim 2, characterized in that, The compound microbial agent includes strains of Arthrobacter xinnanensis with accession number CGMCC 1.8987, strains of Priestella linearis with accession number CGMCC 1.8801, and strains of Bacillus coagulans with accession number CGMCC 1.10823.
4. The microbial agent for improving strawberry yield and quality according to claim 1, characterized in that, The method for preparing the citric acid-sodium citrate buffer solution is as follows: Prepare 0.1 mol / L citric acid solution and 0.1 mol / L sodium citrate solution using deionized water. Mix the citric acid solution and sodium citrate solution at a volume ratio of 1:4 until homogeneous. Adjust the pH to 6.
5. Autoclave the prepared mixed solution at 121℃ for 20 min and then cool it for later use.
5. The microbial agent for improving strawberry yield and quality according to claim 1, characterized in that, The preparation method of the composite microbial agent is as follows: (1) First, activate *Priestella linearis* in nutrient broth agar medium, pick a single colony and inoculate it into nutrient broth liquid medium, and incubate at 30℃ and 170 r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture at a rate of 5% into the nutrient gravy liquid medium in the fermenter, and incubate at 30℃ and 170 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL was added, and the fermentation broth of Priestella lineari was obtained. (2) Activate Arthrobacter xinnanensis in LB solid medium, pick a single colony and inoculate it into LB liquid medium, and culture at 20℃ and 210 r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture into LB liquid medium in the fermenter at a rate of 5% and incubate at 20℃ and 210 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL, and the fermentation broth of Arthrobacter xinnanensis was obtained. (3) Activate Bacillus coagulans in MRS medium, pick a single colony and inoculate it into MRS liquid medium, and culture at 30℃ and 200r / min until OD. 600 =0.6 to obtain seed culture, and inoculate the seed culture at a rate of 1% into the MRS liquid medium in the fermenter and incubate at 30℃ and 200 r / min until the viable count reaches 1×10. 9 The culture was terminated after cfu / mL, and the fermentation broth of Bacillus coagulans was obtained. (4) The prepared Priestella lineari fermentation broth, Arthrobacter xinnani fermentation broth and Bacillus coagulans fermentation broth are mixed in a volume ratio of 3:4:3 to obtain a compound microbial agent.
6. A method for preparing the microbial inoculant for improving strawberry yield and quality as described in claim 1, characterized in that, Includes the following steps: First, prepare a compound microbial agent and a citrate-sodium citrate buffer solution. Place the prepared water in a stirring container, and add xanthan gum, glycerol, and citrate-sodium citrate buffer solution in sequence. Turn on the stirring device and stir until all components are completely dissolved. Continue to add potassium humate, potassium dihydrogen phosphate, and chelated calcium magnesium to the container, and keep stirring until all solid components are completely dispersed and dissolved. Finally, add the prepared compound microbial agent, stir evenly, and you will get a microbial agent to improve strawberry yield and quality.
7. The application of the microbial agent for improving strawberry yield and quality as described in claim 1, characterized in that, The microbial agent described herein can prevent diseases and promote growth in strawberries, significantly improving strawberry yield and quality.
8. The application according to claim 7, characterized in that, Effectively prevents and controls strawberry root rot, strawberry anthracnose, strawberry gray mold, and strawberry wilt.