Preparation method and application of nanocrystallization preparation for preventing and treating Chinese pumpkin leaf curl virus on muskmelon
By using ZnO nanocarriers to load dsRNA into nano-formulations, the problems of stability and high cost in the control of SLCCNV have been solved, achieving precise, green, and efficient virus control in melons and enhancing the disease resistance of plants.
Patent Information
- Application Number
- CN202511627543.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-07
- Publication Date
- 2025-12-05
- Estimated Expiration
- 2045-11-07
AI Technical Summary
Existing technologies for controlling Chinese pumpkin leaf curl virus (SLCCNV) suffer from poor flexibility, high costs, serious chemical pesticide pollution, and ineffective virus control. RNAi technology also suffers from poor dsRNA stability in natural environments, which hinders its widespread application.
We developed a nanoparticle formulation that specifically targets the SLCCNV genome. By loading dsRNA onto a ZnO nanocarrier, we improved its stability and delivery efficiency. Combined with a friction inoculation method, we applied the nanoparticle pesticide to melon leaves to achieve precise targeting and silencing of the viral gene.
It achieves precise and efficient control of SLCCNV, enhances the disease resistance of melons, reduces the risk of using chemical pesticides, protects farmland ecology, adapts to existing cultivation processes, and the nanocarrier promotes the long-term slow release and plant absorption of dsRNA.