53 results about "Cucumber mosaic virus" patented technology

The invention discloses a pCAMBIA1300-mini RNA3-BC-NLPPya vector as well as a construction method and application of the pCAMBIA1300-mini RNA3-BC-NLPPya vector in high cucumber mosaic virus resistance, the vector is characterized in that mini RNA3-BC-NLPPya is constructed to a pCAMBIA1300 vector skeleton, and the gene sequences of mini RNA3-BC and NLPPya are as shown in SEQ ID NO: 2 and SEQ ID NO: 5. The invention finds that the miniRNA3-BC-NLPPya can efficiently inhibit the infection of the CMV and effectively weaken the harm caused by the CMV, does not need to apply any medicine, is an efficient and green disease-resistant technology, and can provide a new strategy for preventing and controlling the cucumber mosaic virus and similar viruses thereof.

The invention provides a coumarin derivative containing oxadiazole (thiadiazole) thioether and a preparation method and application thereof, the structural general formula of the coumarin derivative containing oxadiazole (thiadiazole) thioether is shown as I, and the structural general formula of the coumarin derivative containing thiadiazole thioether is shown as II, the synthesis route of the coumarin derivative containing oxadiazole (thiadiazole) thioether is simple and practical, the preparation raw materials are easy to obtain, the cost is low, and the prepared compound has a good control effect on plant viruses including tobacco mosaic virus, cucumber mosaic virus, potato Y virus, southern rice black-streaked dwarf virus or rice stripe virus. According to the invention, a favorable lead compound is provided for the creation of a natural bionic antiviral agent; # imgabs0 #

The invention relates to a plant source biological control agent for controlling cucumber mosaic virus and application thereof. The plant source biological control agent is one or a combination of any two or three of garlic, wedelia chinensis and mikania micrantha. Researches show that the combination of one, any two or three of garlic, wedelia chinensis and mikania micrantha has a remarkable inhibition effect on cucumber mosaic virus, and the combined use effect of garlic, wedelia chinensis and mikania micrantha is remarkably improved. According to the invention, more safe and efficient plant source control agents are provided for controlling plant diseases caused by cucumber mosaic virus, and a foundation is laid for subsequent research and development of novel cucumber mosaic virus resistant plant source pesticides. Moreover, the garlic, the wedelia chinensis and the mikania micrantha are wide in source and low in cost, and have very good application value.

The application provides a plasmid containing modified satellite RNA and a construction method and application, and belongs to the field of plant virology and molecular biology. Each copy number of the modified satellite RNA is composed of a modified satRNA TA-Tb fragment and an HDV ribozyme fragment, and the copy number of the modified satellite RNA in the plasmid is 10. The nucleotide sequence of the modified satellite RNA is shown in SEQ No. 3. After the plasmid of the application is inoculated by agrobacterium injection, the symptoms caused by cucumber mosaic virus on different strains and the same strain (CMV Fny ) on different hosts can be significantly reduced. An effective control measure for field control of cucumber mosaic virus is provided.

The present invention relates to compositions comprising modified virus-like particles (VLPs) of Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising chimeric CMV polypeptides which comprise a stretch of consecutive negative amino acids selected from aspartic acid and / or glutamic acid to which specific Interleukin- 1β mutein antigens, IL-1β-D145X antigens, are linked, as well as pharmaceutical compositions thereof, which compositions preferably serve as vaccines for generating immune responses, in particular antibody responses, against IL-1β.

This invention provides one use of borneol as an aphid repellent, specifically for the control of tobacco aphids. This invention also provides a method for repelling tobacco aphids by using borneol as a repellent. After analyzing the volatile components of tobacco infected with cucumber mosaic virus, this invention discovered that borneol has aphid-repelling effect and can be used to prepare repellents, thus providing a new compound for aphid control.

This invention discloses a pCAMBIA1300-miniRNA3-BC-NLP Pya Vector and construction method and its application in high resistance to cucumber mosaic virus: The vector is miniRNA3-BC-NLP. Pya Construct the pCAMBIA1300 vector backbone, containing miniRNA3-BC and NLP. Pya The gene sequences are shown in SEQ ID NO:2 and 5. This invention discovered miniRNA3-BC-NLP. Pya It can effectively inhibit CMV infection and reduce the harm caused by CMV without the need for any drugs. It is a highly efficient and green disease control technology that can provide a new strategy for the prevention and control of cucumber mosaic virus and similar viruses.

The present invention relates to veterinary compositions comprising NGF antigen linked to modified virus-like particles (VLPs) of Cucumber Mosaic Virus (CMV), in particular to modified VLPs of CMV comprising chimeric CMV polypeptides for use in a method of treating a NGF-related disorder in canine, in particular in the treatment of pain, such as for example pain associated with osteoarthritis (OA) in dogs

The present invention relates to compositions comprising modified virus-like particles (VLPs) of Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising chimeric CMV polypeptides which comprise a stretch of consecutive negative amino acids selected from aspartic acid and / or glutamic acid to which canine Interleukin-1β mutein antigens, cIL-1β-D145X antigens, are linked, as well as pharmaceutical compositions thereof, which compositions preferably serve as vaccines for generating immune responses, in particular antibody responses, against cIL-1β.

The present invention relates to compositions comprising modified virus-like particles (VLPs) of Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising chimeric CMV polypeptides which comprises a stretch of consecutive negative amino acids selected from aspartic acid or glutamic acid to which nerve growth factor (NGF) antigens are linked as well as pharmaceutical compositions thereof, which compositions preferably serve as vaccine platform for generating immune responses, in particular antibody responses, against said NGF antigens linked to the modified CMV VLPs.

A new and distinct plant of variety NUN 83071 CUL is disclosed as well as seeds and plants and fruits thereof. NUN 83071 CUL is a Dutch long cucumber variety comprising resistance to Cladosporium cucumerinum, Cucumber Mosaic Virus (CMV), Podosphaera xanthii, Corynespora cassiicola, Cucumber Vein Yellowing Virus (CVYV), and Cucumber Green Mottle Mosaic Virus (CGMMV).

A new and distinct plant of variety NUN 53063 CUP is disclosed as well as seeds and plants and fruits thereof. NUN 53063 CUP is a pickling cucumber variety comprising resistance to Cladosporium cucumerinum, Podosphaera xanthii, and Cucumber Mosaic Virus.

The invention relates to the technical field of agricultural biology, and particularly provides a preparation method and application of a nanocrystallization preparation for preventing and treating Chinese pumpkin leaf curl virus on muskmelons. Based on an RNAi interference technology, an efficient target gene region DNA-A-AV2 for inhibiting virus infection is screened, corresponding dsRNA is induced and synthesized, zinc oxide (ZnO) is used for loading and then is delivered into a plant body, the stability of the dsRNA is improved, long-acting slow release is realized, and the prevention and treatment effect of the dsRNA is enhanced. Experiments show that the nano pesticide can effectively inhibit replication and diffusion of viruses and reduce the content of the viruses. Compared with traditional chemical agents and conventional breeding, the method has irreplaceable advantages in pertinence, safety and adaptability, and meets the requirements of current agriculture for'precision 'and'green'.

The present invention relates to compositions comprising modified virus-like particles (VLPs) of Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising chimeric CMV polypeptides which preferably comprise a stretch of consecutive negative amino acids selected from aspartic acid and / or glutamic acid to which specific feline Interleukin-1β mutein antigens, fIL-1β-D145X antigens, are linked, as well as pharmaceutical compositions thereof, which compositions preferably serve as vaccines for generating immune responses, in particular antibody responses, against fIL-1β.

The invention discloses an arabidopsis thaliana VHA-B1 gene, a coding protein thereof, a mutant, and preparation, identification and application thereof, and the preparation of the mutant comprises the following steps: respectively targeting a ninth exon and / or a first exon of a VHA-B1 gene (such as SEQ ID NO.1) through a T-DNA insertion and / or gene editing technology to obtain an insertion mutant and / or a gene editing mutant, and carrying out amplification on the insertion mutant and / or the gene editing mutant to obtain the VHA-B1 gene. And identifying the insertion mutant and / or the gene editing mutant through PCR (Polymerase Chain Reaction) to obtain the homozygous mutant with VHA-B1 gene deletion. The tolerance of the obtained purified mutant to cucumber mosaic virus (CMV) is weakened, which shows that the Arabidopsis thaliana VHA-B1 gene can improve the CMV tolerance of the plant, and an application basis is provided for culturing antiviral crop varieties by using the VHA-B1 gene.

The invention discloses a preparation method of a novel plant virus resisting agent and a derivative thereof, and application of the novel plant virus resisting agent and the derivative in foliar fertilizer. The general formula of the derivative is shown in the specification, wherein R is halogen, methyl, methoxyl or disubstituted or trisubstituted of any combination of the above substituent groups. The derivative disclosed by the invention has a relatively good inhibition effect on tobacco mosaic virus diseases, cucumber mosaic virus diseases and potato Y virus diseases. The compound II-15 has a relatively good inhibition effect on three virus diseases.

The invention provides a preparation method and application of an astragalus extract-containing pesticide for preventing and treating plant viruses, the preparation method takes traditional Chinese medicine astragalus as a raw material, and comprises the steps of alcohol reflux extraction, adsorption of macroporous adsorption resin and a TPE transparent material, water washing, washing with a diluted alcohol solution, adsorption of silica gel and the TPE transparent material, elution of astragaloside with a high-concentration alcohol solution, concentration and crystallization. The astragaloside prepared by the method is light in color and high in purity, the preparation method is high in yield, the prepared astragaloside is used for treating the tobacco mosaic virus and the cucumber mosaic virus, and experiments show that the astragaloside has an inhibition effect on common plant viruses such as the tobacco mosaic virus (TMV) and the cucumber mosaic virus (CMV), and the astragaloside can be used for treating the tobacco mosaic virus and the cucumber mosaic virus. Particularly, astragaloside, xinjunan and moroxydine hydrochloride are used to enhance the antiviral effect of the pesticide.

The present invention relates to a modified virus-like particle (VLP) of cucumber mosaic virus (CMV) comprising at least one chimeric CMV polypeptide, wherein said at least one chimeric CMV polypeptide comprises, preferably consists of (i) a CMV polypeptide, wherein said CMV polypeptide comprises a coat protein of CMV or an amino acid sequence having a sequence identity of at least 75% with SEQ ID NO:48; and (ii) a polypeptide comprising, preferably consisting of, a stretch of consecutive negative amino acids, wherein said negative amino acids are independently selected from aspartic acid or glutamic acid, wherein said polypeptide is inserted between any amino acid residue of said CMV polypeptide corresponding to any amino acid residue between position 75 and position 85 of SEQ ID NO:48, as well as to compositions and pharmaceutical compositions comprising such modified VLPs to which antigens are linked, which compositions preferably serve as vaccine platform for generating immune responses, in particular antibody responses, against said antigens linked to the modified CMV VLPs.

The present invention relates to compositions comprising modified virus-like particles (VLPs) of Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising chimeric CMV polypeptides which comprise a stretch of consecutive negative amino acids selected from aspartic acid and / or glutamic acid to which canine Interleukin-1β mutein antigens, cIL-1β-D145X antigens, are linked, as well as pharmaceutical compositions thereof, which compositions preferably serve as vaccines for generating immune responses, in particular antibody responses, against cIL-1β.

The application discloses a cucumber mosaic virus photoelectric chemical sensor and a detection method thereof, and belongs to the technical field of photoelectric chemical sensors.The existing PEC sensor has the problems of low photoelectric conversion efficiency and lack of efficient signal amplification strategy.The sensor provided by the application takes a screen-printed electrode as a substrate, a three-dimensional flower-shaped ZnIn2S4 nanomaterial is used as a light active layer to modify a working electrode, and a basic phosphatase-mediated enzymatic biological catalytic precipitation signal amplification system is matched;the detection method adopts a sandwich immunization mode to capture viruses, catalyzes the generation of BCP precipitation to shield the transmission of photo-generated carriers, and quantitatively detects through photocurrent attenuation, the linear range of the detection is 0.001-200 ng / mL, and the detection limit is 0.001 ng / mL, so that the cucumber mosaic virus can be rapidly screened in a field.

The invention discloses a preparation method of a novel coumarin derivative containing a benzylamine structure and application of the novel coumarin derivative in the aspects of virus resistance, bacteriostasis and plant growth promotion. The novel coumarin derivative containing the benzylamine structure is characterized in that the general formula of the derivative is shown in the specification, wherein R1 is halogen, methoxyl, nitryl, methyl, cyano, tert-butyl or disubstitution of any combination of the above substituent groups. The derivative disclosed by the invention has a relatively good inhibition effect on rice bacterial leaf blight, tobacco bacterial wilt, potato soft rot, tobacco mosaic virus disease and cucumber mosaic virus disease. The compound II-8 has the activity of promoting plant growth.

The present invention relates to compositions comprising modified virus-like particles (VLPs) of Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising chimeric CMV polypeptides which comprise a stretch of consecutive negative amino acids selected from aspartic acid and / or glutamic acid to which specific Interleukin- 1β mutein antigens, IL-1β-D145X antigens, are linked, as well as pharmaceutical compositions thereof, which compositions preferably serve as vaccines for generating immune responses, in particular antibody responses, against IL-1β.

The present invention provides a trivalent attenuated vaccine against PVY, PVX and CMV and its application, and the present invention belongs to the field of plant virology and molecular biology. The trivalent attenuated vaccine contains a mutant plasmid pCB3 of cucumber mosaic virus (CMV) RNA2. F R2‑2bPT‑PVY 8093‑8292 PVX 85‑284 The sequence of the mutant plasmid is shown in SEQ ID NO.3. Fny Plasmid pCB from isolate RNA1 F R1 and CMV Fny Isolate RNA3 aphid-transmitted site mutation plasmid pCB F R3‑△apno is pre-mixed with mutant plasmids for inoculation to prevent potato virus Y, potato virus X and cucumber mosaic virus. At the same time, the vaccine can prevent aphids from transmitting the virus and avoid biosafety risks, providing vaccine materials and effective measures for field control of PVY, PVX and CMV.

The present invention relates to compositions comprising modified virus-like particles (VLPs) of Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising chimeric CMV polypeptides which preferably comprise a stretch of consecutive negative amino acids selected from aspartic acid and / or glutamic acid to which specific feline Interleukin-1β mutein antigens, fIL-1β-D145X antigens, are linked, as well as pharmaceutical compositions thereof, which compositions preferably serve as vaccines for generating immune responses, in particular antibody responses, against fIL-1β.

The application provides a method for efficiently and stably inoculating tomatoes with cucumber mosaic virus, and relates to the technical field of crop disease-resistant breeding.The method for efficiently and stably inoculating tomatoes with cucumber mosaic virus comprises the following specific steps: S1, propagation: propagating CMV virus by using dcl 2 / 4i n.benthamiana; S2, extraction: extracting CMV virions; S3, inoculation: rubbing the tomatoes seedlings with the CMV virions in S2; and S4, cultivation: cultivating the tomatoes seedlings. The method significantly improves the efficiency and stability of CMV infection of tomatoes by using virions to infect the tomatoes seedlings. Furthermore, the dcl 2 / 4i n.benthamiana is used to propagate CMV virions, thereby shortening the propagation cycle of CMV virions, significantly improving the yield of CMV virions, and enabling repeated large-scale inoculation, which is conducive to efficient screening of CMV-resistant tomato varieties.

The invention discloses an application of a terpenoid natural product containing an isoprene unit as an agricultural bactericide in plant diseases, the terpenoid natural product containing the isoprene unit is composed of two or more isoprene units, and the terpenoid natural product containing the isoprene unit aims at plant viral diseases such as tobacco mosaic virus, cucumber mosaic virus or potato Y virus; aiming at plant fungal diseases such as cucumber gray mold, pepper fusarium wilt, sclerotinia rot of colza, wheat scab, potato late blight, blueberry root rot, botryosphaeria dothidea, pitaya anthracnose, rice sheath blight disease, fusarium SP or colletotrichum capsici; the compound has a good inhibition effect on plant bacterial diseases such as rice bacterial leaf blight, citrus canker, tobacco bacterial wilt, kiwifruit canker or rice bacterial leaf streak. The invention provides new application of terpenoid natural products, and the terpenoid natural products can be used for preventing and treating plant bacterial diseases, fungal diseases and plant viral diseases in the agricultural field.

The present invention provides a method for preventing and controlling cucumber mosaic virus-induced leaf disease in cut lilies, characterized by comprising the following steps: 1) germinating lily bulbs, 2) planting the lily bulbs, 3) watering the lily bulbs, 4) watering the lily bulbs, 5) irrigating the lily bulbs with a microbial fertilizer solution, and 6) irrigating the lily bulbs with the microbial fertilizer solution, thereby obtaining cut lily plants. This method maximizes the lily plants' resistance and immunity to cucumber mosaic virus, reducing the incidence of cucumber mosaic virus to below 5%. This method maximizes the commercial value of lily bulbs carrying cucumber mosaic virus, avoids the significant economic losses to producers caused by the destruction of such bulbs or plants, and reduces fertilizer usage by over 30%. The microbial fertilizer used is environmentally friendly, ensuring a green and environmentally friendly cultivation process and overall quality assurance for cut lilies.

The application belongs to the field of plant genetic engineering, and discloses a pepper cucumber mosaic virus disease resistance related gene and application thereof. The application constructs overexpression plants of pepper gene Capana00g000026 and silencing plants of the pepper gene Capana00g000026, identifies characteristics of the plants after inoculation of the cucumber mosaic virus, and analyzes the role of the pepper gene Capana00g000026 in the defense of the pepper infected with the cucumber mosaic virus; the genes related to the defense are screened; it is proved that increasing the content of the gene can significantly reduce the accumulation amount of the virus in the pepper leaves, and the disease phenotype of the plant is weakened, so the gene can be applied to pepper virus resistance engineering. The gene can be applied to the pepper, and the breeding material with CMV resistance can be cultivated by transformation of the pepper material.

The present application relates to a coumarin derivative containing a dithioacetal unit, a preparation method and use thereof. The coumarin derivative has excellent antiviral activity against cucumber mosaic virus and the like, and can be used for preparing a medicine or agent for preventing and treating plant viral diseases such as cucumber mosaic virus, and has simple structure and preparation process.

Provided herein are spinach plants resistant to Peronospora farinosa and Stemphylium vesicarium, and spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). Also provided herein are genomic fragments providing the present resistances and use thereof for identifying spinach resistant to Peronospora farinosa and Stemphylium vesicarium, and spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). Provided herein are a spinach plant resistant to Peronospora farinosa and Stemphylium vesicarium: including a genomic fragment of Spinacia tetrandra located on chromosome 4 between positions 8255074 and 8620598 of the spinach reference genome, the genomic fragment of Spinacia tetrandra lacking a lethal factor and providing Peronospora farinosa resistance and including a genomic fragment located on chromosome 3 and between positions 1177586 and 1271037 of the spinach reference genome providing Stemphylium vesicarium resistance.