28 results about "Cucumber mosaic virus" patented technology

The invention relates to a plant source biological control agent for controlling cucumber mosaic virus and application thereof. The plant source biological control agent is one or a combination of any two or three of garlic, wedelia chinensis and mikania micrantha. Researches show that the combination of one, any two or three of garlic, wedelia chinensis and mikania micrantha has a remarkable inhibition effect on cucumber mosaic virus, and the combined use effect of garlic, wedelia chinensis and mikania micrantha is remarkably improved. According to the invention, more safe and efficient plant source control agents are provided for controlling plant diseases caused by cucumber mosaic virus, and a foundation is laid for subsequent research and development of novel cucumber mosaic virus resistant plant source pesticides. Moreover, the garlic, the wedelia chinensis and the mikania micrantha are wide in source and low in cost, and have very good application value.

The application provides a plasmid containing modified satellite RNA and a construction method and application, and belongs to the field of plant virology and molecular biology. Each copy number of the modified satellite RNA is composed of a modified satRNA TA-Tb fragment and an HDV ribozyme fragment, and the copy number of the modified satellite RNA in the plasmid is 10. The nucleotide sequence of the modified satellite RNA is shown in SEQ No. 3. After the plasmid of the application is inoculated by agrobacterium injection, the symptoms caused by cucumber mosaic virus on different strains and the same strain (CMV Fny ) on different hosts can be significantly reduced. An effective control measure for field control of cucumber mosaic virus is provided.

This invention provides one use of borneol as an aphid repellent, specifically for the control of tobacco aphids. This invention also provides a method for repelling tobacco aphids by using borneol as a repellent. After analyzing the volatile components of tobacco infected with cucumber mosaic virus, this invention discovered that borneol has aphid-repelling effect and can be used to prepare repellents, thus providing a new compound for aphid control.

This invention discloses a pCAMBIA1300-miniRNA3-BC-NLP Pya Vector and construction method and its application in high resistance to cucumber mosaic virus: The vector is miniRNA3-BC-NLP. Pya Construct the pCAMBIA1300 vector backbone, containing miniRNA3-BC and NLP. Pya The gene sequences are shown in SEQ ID NO:2 and 5. This invention discovered miniRNA3-BC-NLP. Pya It can effectively inhibit CMV infection and reduce the harm caused by CMV without the need for any drugs. It is a highly efficient and green disease control technology that can provide a new strategy for the prevention and control of cucumber mosaic virus and similar viruses.

The present invention relates to compositions comprising modified virus-like particles (VLPs) of Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising chimeric CMV polypeptides which comprises a stretch of consecutive negative amino acids selected from aspartic acid or glutamic acid to which nerve growth factor (NGF) antigens are linked as well as pharmaceutical compositions thereof, which compositions preferably serve as vaccine platform for generating immune responses, in particular antibody responses, against said NGF antigens linked to the modified CMV VLPs.

A new and distinct plant of variety NUN 83071 CUL is disclosed as well as seeds and plants and fruits thereof. NUN 83071 CUL is a Dutch long cucumber variety comprising resistance to Cladosporium cucumerinum, Cucumber Mosaic Virus (CMV), Podosphaera xanthii, Corynespora cassiicola, Cucumber Vein Yellowing Virus (CVYV), and Cucumber Green Mottle Mosaic Virus (CGMMV).

The invention relates to the technical field of agricultural biology, and particularly provides a preparation method and application of a nanocrystallization preparation for preventing and treating Chinese pumpkin leaf curl virus on muskmelons. Based on an RNAi interference technology, an efficient target gene region DNA-A-AV2 for inhibiting virus infection is screened, corresponding dsRNA is induced and synthesized, zinc oxide (ZnO) is used for loading and then is delivered into a plant body, the stability of the dsRNA is improved, long-acting slow release is realized, and the prevention and treatment effect of the dsRNA is enhanced. Experiments show that the nano pesticide can effectively inhibit replication and diffusion of viruses and reduce the content of the viruses. Compared with traditional chemical agents and conventional breeding, the method has irreplaceable advantages in pertinence, safety and adaptability, and meets the requirements of current agriculture for'precision 'and'green'.

The invention discloses a preparation method of a novel plant virus resisting agent and a derivative thereof, and application of the novel plant virus resisting agent and the derivative in foliar fertilizer. The general formula of the derivative is shown in the specification, wherein R is halogen, methyl, methoxyl or disubstituted or trisubstituted of any combination of the above substituent groups. The derivative disclosed by the invention has a relatively good inhibition effect on tobacco mosaic virus diseases, cucumber mosaic virus diseases and potato Y virus diseases. The compound II-15 has a relatively good inhibition effect on three virus diseases.

The invention provides a preparation method and application of an astragalus extract-containing pesticide for preventing and treating plant viruses, the preparation method takes traditional Chinese medicine astragalus as a raw material, and comprises the steps of alcohol reflux extraction, adsorption of macroporous adsorption resin and a TPE transparent material, water washing, washing with a diluted alcohol solution, adsorption of silica gel and the TPE transparent material, elution of astragaloside with a high-concentration alcohol solution, concentration and crystallization. The astragaloside prepared by the method is light in color and high in purity, the preparation method is high in yield, the prepared astragaloside is used for treating the tobacco mosaic virus and the cucumber mosaic virus, and experiments show that the astragaloside has an inhibition effect on common plant viruses such as the tobacco mosaic virus (TMV) and the cucumber mosaic virus (CMV), and the astragaloside can be used for treating the tobacco mosaic virus and the cucumber mosaic virus. Particularly, astragaloside, xinjunan and moroxydine hydrochloride are used to enhance the antiviral effect of the pesticide.

The present invention relates to a modified virus-like particle (VLP) of cucumber mosaic virus (CMV) comprising at least one chimeric CMV polypeptide, wherein said at least one chimeric CMV polypeptide comprises, preferably consists of (i) a CMV polypeptide, wherein said CMV polypeptide comprises a coat protein of CMV or an amino acid sequence having a sequence identity of at least 75% with SEQ ID NO:48; and (ii) a polypeptide comprising, preferably consisting of, a stretch of consecutive negative amino acids, wherein said negative amino acids are independently selected from aspartic acid or glutamic acid, wherein said polypeptide is inserted between any amino acid residue of said CMV polypeptide corresponding to any amino acid residue between position 75 and position 85 of SEQ ID NO:48, as well as to compositions and pharmaceutical compositions comprising such modified VLPs to which antigens are linked, which compositions preferably serve as vaccine platform for generating immune responses, in particular antibody responses, against said antigens linked to the modified CMV VLPs.

The application discloses a cucumber mosaic virus photoelectric chemical sensor and a detection method thereof, and belongs to the technical field of photoelectric chemical sensors.The existing PEC sensor has the problems of low photoelectric conversion efficiency and lack of efficient signal amplification strategy.The sensor provided by the application takes a screen-printed electrode as a substrate, a three-dimensional flower-shaped ZnIn2S4 nanomaterial is used as a light active layer to modify a working electrode, and a basic phosphatase-mediated enzymatic biological catalytic precipitation signal amplification system is matched;the detection method adopts a sandwich immunization mode to capture viruses, catalyzes the generation of BCP precipitation to shield the transmission of photo-generated carriers, and quantitatively detects through photocurrent attenuation, the linear range of the detection is 0.001-200 ng / mL, and the detection limit is 0.001 ng / mL, so that the cucumber mosaic virus can be rapidly screened in a field.

The invention discloses a preparation method of a novel coumarin derivative containing a benzylamine structure and application of the novel coumarin derivative in the aspects of virus resistance, bacteriostasis and plant growth promotion. The novel coumarin derivative containing the benzylamine structure is characterized in that the general formula of the derivative is shown in the specification, wherein R1 is halogen, methoxyl, nitryl, methyl, cyano, tert-butyl or disubstitution of any combination of the above substituent groups. The derivative disclosed by the invention has a relatively good inhibition effect on rice bacterial leaf blight, tobacco bacterial wilt, potato soft rot, tobacco mosaic virus disease and cucumber mosaic virus disease. The compound II-8 has the activity of promoting plant growth.

The application provides a method for efficiently and stably inoculating tomatoes with cucumber mosaic virus, and relates to the technical field of crop disease-resistant breeding.The method for efficiently and stably inoculating tomatoes with cucumber mosaic virus comprises the following specific steps: S1, propagation: propagating CMV virus by using dcl 2 / 4i n.benthamiana; S2, extraction: extracting CMV virions; S3, inoculation: rubbing the tomatoes seedlings with the CMV virions in S2; and S4, cultivation: cultivating the tomatoes seedlings. The method significantly improves the efficiency and stability of CMV infection of tomatoes by using virions to infect the tomatoes seedlings. Furthermore, the dcl 2 / 4i n.benthamiana is used to propagate CMV virions, thereby shortening the propagation cycle of CMV virions, significantly improving the yield of CMV virions, and enabling repeated large-scale inoculation, which is conducive to efficient screening of CMV-resistant tomato varieties.

The application belongs to the field of plant genetic engineering, and discloses a pepper cucumber mosaic virus disease resistance related gene and application thereof. The application constructs overexpression plants of pepper gene Capana00g000026 and silencing plants of the pepper gene Capana00g000026, identifies characteristics of the plants after inoculation of the cucumber mosaic virus, and analyzes the role of the pepper gene Capana00g000026 in the defense of the pepper infected with the cucumber mosaic virus; the genes related to the defense are screened; it is proved that increasing the content of the gene can significantly reduce the accumulation amount of the virus in the pepper leaves, and the disease phenotype of the plant is weakened, so the gene can be applied to pepper virus resistance engineering. The gene can be applied to the pepper, and the breeding material with CMV resistance can be cultivated by transformation of the pepper material.

The present application relates to a coumarin derivative containing a dithioacetal unit, a preparation method and use thereof. The coumarin derivative has excellent antiviral activity against cucumber mosaic virus and the like, and can be used for preparing a medicine or agent for preventing and treating plant viral diseases such as cucumber mosaic virus, and has simple structure and preparation process.

Provided herein are spinach plants resistant to Peronospora farinosa and Stemphylium vesicarium, and spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). Also provided herein are genomic fragments providing the present resistances and use thereof for identifying spinach resistant to Peronospora farinosa and Stemphylium vesicarium, and spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). Provided herein are a spinach plant resistant to Peronospora farinosa and Stemphylium vesicarium: including a genomic fragment of Spinacia tetrandra located on chromosome 4 between positions 8255074 and 8620598 of the spinach reference genome, the genomic fragment of Spinacia tetrandra lacking a lethal factor and providing Peronospora farinosa resistance and including a genomic fragment located on chromosome 3 and between positions 1177586 and 1271037 of the spinach reference genome providing Stemphylium vesicarium resistance.

One aspect relates to an improved recombinant vector based on the cucumber mosaic virus. The recombinant vector according to one embodiment can significantly increase the efficiency of foreign protein expression in plants, and has a significantly higher efficiency of foreign protein expression than existing recombinant vectors, and can therefore be widely used in the production and research of useful proteins in various plant species.

The present invention relates to a modified virus-like particle (VLP) of Cucumber Mosaic Virus (CMV) comprising at least one fusion protein, wherein the at least one fusion protein comprises or preferably consists of b) a chimeric CMV polypeptide, wherein the chimeric CMV polypeptide comprises or preferably consists of (iii) a CMV polypeptide, wherein the CMV polypeptide comprises a coat protein of CMV; and (iv) an antigen polypeptide, wherein the antigen polypeptide is inserted into the CMV polypeptide, wherein the insertion of the antigen polypeptide is between amino acid residues corresponding to position 84 and position 85 of SEQ ID NO: 62 of the CMV polypeptide; and (iii) a T helper cell epitope, wherein the T helper cell epitope replaces an N-terminal region of the CMV polypeptide, and wherein preferably the N-terminal region of the CMV polypeptide corresponds to amino acids 2-12 of SEQ ID NO: 62.

The application belongs to the technical field of biology, and particularly relates to a preparation method of artificial defective interfering RNA and application thereof in resisting cucumber mosaic virus. The application discloses an artificial defective interfering RNA, which comprises the following elements in the direction from the 5' end to the 3' end: a 5' end UTR of a CMV genomic RNA3, an IGR, a CP open reading frame and a 3' end UTR sequence. The application also simultaneously provides a recombinant DNA for coding the artificial defective interfering RNA. The application also simultaneously provides a recombinant expression vector containing the above-mentioned recombinant DNA. The application also simultaneously provides application of the above-mentioned artificial defective interfering RNA, the recombinant DNA and the recombinant expression vector in resisting cucumber mosaic virus.

The present invention relates to compositions comprising modified virus-like particles (VLPs) of Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising chimeric CMV polypeptides which preferably comprise a stretch of consecutive negative amino acids selected from aspartic acid and / or glutamic acid to which specific feline Interleukin-1β mutein antigens, fIL-1β-D145X antigens, are linked, as well as pharmaceutical compositions thereof, which compositions preferably serve as vaccines for generating immune responses, in particular antibody responses, against fIL-1β.

The present invention relates to compositions comprising modified virus-like particles (VLPs) of Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising chimeric CMV polypeptides which preferably comprise a stretch of consecutive negative amino acids selected from aspartic acid and / or glutamic acid to which specific feline Interleukin-1β mutein antigens, fIL-1β-D145X antigens, are linked, as well as pharmaceutical compositions thereof, which compositions preferably serve as vaccines for generating immune responses, in particular antibody responses, against fIL-1β.

Embodiments relate to an improved recombinant vector based on cucumber mosaic virus. The recombinant vector according to the embodiments can significantly improve the expression efficiency of foreign proteins in plants, has remarkably higher foreign protein expression efficiency than existing recombinant vectors, and thus can be widely used for the production of useful proteins and research thereof in various plant species.

The present invention relates to virus-like particles of plant virus Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising Th cell epitopes, in particular universal Th cell epitopes. Furthermore, these modified VLPs serve as, preferably, vaccine platform, for generating immune responses, in particular antibody responses, against antigens linked to said modified VLPs. The presence of the Th cell epitopes, in particular universal Th cell epitopes, led to a further increase in the generated immune response.

The present invention relates to a modified virus-like particle (VLP) of Cucumber Mosaic Virus (CMV) comprising at least one fusion protein, wherein the at least one fusion protein comprises or preferably consists of b) a chimeric CMV polypeptide, wherein the chimeric CMV polypeptide comprises or preferably consists of (iii) a CMV polypeptide, wherein the CMV polypeptide comprises or preferably consists of a coat protein of CMV, wherein preferably the coat protein of CMV comprises or preferably consists of SEQ ID NO: 62; or an amino acid sequence having a sequence identity of at least 75%, preferably at least 80%, more preferably at least 85%, further preferably at least 90%, yet further preferably at least 95%, still further preferably at least 98% and yet still further more preferably at least 99% to SEQ ID NO: 62; and (iv) an antigen polypeptide, wherein the antigen polypeptide is inserted into the CMV polypeptide, wherein the insertion of the antigen polypeptide is between amino acid residues corresponding to amino acid residue of position 84 and position 85 of SEQ ID NO: 62 of the CMV polypeptide; and (iii) a T-helper cell epitope, wherein the T-helper cell epitope replaces an N-terminal region of the CMV polypeptide, and wherein preferably the N-terminal region of the CMV polypeptide corresponds to amino acids 2-12 of SEQ ID NO: 62.

The application relates to the field of agricultural biotechnology, and specifically provides a preparation method and application of a nanoized preparation for preventing and treating Chinese squash leaf curl virus on melons. The application is based on RNAi interference technology, screens a high-efficiency target gene region DNA-A-AV2 for inhibiting virus infection, induces synthesis of corresponding dsRNA, and delivers the dsRNA into a plant body after being loaded with zinc oxide (ZnO), so that the stability of the dsRNA is improved, long-acting slow release is realized, and the prevention and treatment effect of the dsRNA is strengthened. Experiments show that the nano pesticide can effectively inhibit virus replication and spread, and reduce the virus content. Compared with traditional chemical agents and conventional breeding, the application has irreplaceable advantages in terms of pertinence, safety and adaptability, and meets the current agricultural "precision" and "green" requirements.

The invention relates to the technical field of seed treatment, in particular to a method for removing cucumber mosaic virus in bitter gourd seeds, which comprises the following steps: step 1, cellulase pretreatment; step 2, irradiation treatment of nano particles and light: soaking the seeds treated in the step 1 in a mixed solution of a nano particle solution coated with a photosensitizer, protease K and lauryl sodium sulfate, then adding a hydrogen peroxide solution for soaking, irradiating with light matched with the maximum absorption wavelength of the photosensitizer, and cleaning after irradiation is completed; step 3, drying: drying the seeds cleaned in the step 2; step 4, germination detection: taking part of the seeds obtained in the step 3 for germination test, and observing and recording the germination rate of the seeds; and 5, RT-LAMP detection: carrying out cucumber mosaic virus detection on the seedlings obtained in the step 4 by adopting an RT-LAMP method, and carrying out multiple treatment, so that the cucumber mosaic virus in the bitter gourd seeds can be effectively removed.

The invention provides O-type foot-and-mouth disease mosaic nanoparticles based on a cucumber mosaic virus capsid protein carrier, and belongs to the technical field of veterinary vaccines. The invention provides O-type foot-and-mouth disease mosaic nanoparticles based on a cucumber mosaic virus capsid protein (CuMVtt) carrier. The O-type foot-and-mouth disease mosaic nanoparticles are obtained by fusion expression of CuMVtt and a plurality of VP1G-H-loop tandem cyclic peptides derived from O-type FMDV. The O-type foot-and-mouth disease mosaic nano-particles are further prepared into O-type foot-and-mouth disease mosaic nano-particle vaccines. The O-type foot-and-mouth disease mosaic nanoparticles can be subjected to fusion expression through a prokaryotic expression system, and an O-type foot-and-mouth disease mosaic nanoparticle vaccine prepared by matching with an ISA201VG adjuvant has a remarkable immune effect on pigs, so that a safe, efficient and broad-spectrum candidate vaccine is provided for subsequent prevention and control of O-type foot-and-mouth disease.

A plant-derived biocontrol agent for controlling cucumber mosaic virus disease includes one or more of the following: Alternanthera philoxeroides, Bidens trifoliata, Rhododendron simsii, Houttuynia cordata, or Mentha haplocalyx. Research in this invention shows that Alternanthera philoxeroides, Bidens trifoliata, Rhododendron simsii, Houttuynia cordata, and Mentha haplocalyx have certain inhibitory effects on cucumber mosaic virus, with Alternanthera philoxeroides showing the most significant inhibitory effect. This invention provides more options for controlling cucumber mosaic virus disease.