224 results about "Cucumber mosaic virus" patented technology

The invention provides a pesticide composition, and in particular relates to an active pesticide composition, compounding one or more of toxic fluoride phosphate, the weight ratio of toxic fluoride phosphate to other active substances is 30:0.15 to 3.3:30, and the content of effective components by weight is 1%-90%. The compound composition can be processed into water dispersible powder, suspending agent, microemulsion, aqueous emulsion, water dispersible granule, dry suspension emulsion and seed coating agent and the like, after accessory ingredient or carrying agent is added. The composition can be used to prevent and treat virus diseases caused by rice stripe virus, rice dwarf virus, maze rough dwarf virus, rice black-streaked dwarf virus, tomato virus, cucumber mosaic virus and tobacco mosaic virus, and has the beneficial effects of enhanced effect, concurrent treatment, postponed pesticide resistance, decreased dosage of toxic fluoride phosphate and reduced cost.

The invention provides a formulation composite comprising toxic fluoride phosphate and at least one growth regulator. According to the weight part, the toxic fluoride phosphate accounts for 10%-80% of the formulation composite, the growth regulator accounts for 0.0001%-2% of the formulation composite, the formulation composite also comprises an assistant, an surface active agent, a solvent and a carrier accepted by agriculture, and the formulation composite can be prepared into wettable powder, suspending agent, missible oil, microemulsion, emulsion in water, water dispersible granule, dry suspending agent, seed coating agent and other agents. The formulation composite can be used for preventing and curing tobacco mosaic virus, cucumber mosaic virus, and various virus diseases caused by viruses on rice, such as tobacco mosaic, cucumber mosaic, tomato virus disease, pepper virus disease, rice virus disease and the like and achieves the effects of improving efficacy, treating in a compatible manner, delaying medicine resistance, reducing the using amount of toxic fluoride phosphate and the like.

The invention relates to a method for detecting tobacco viruses by a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) technology, which comprises the steps of tobacco virus total ribonucleic acid (RNA) extraction, RT-LAMP reaction, electrophoresis detection and virus type determination, wherein tobacco viruses are selected from two or more than two kinds of viruses from cucumber mosaic viruses (CMV), potato viruses Y (PVY), tobacco etch viruses (TEV), tobacco mosaic viruses (TMV) and tobacco vein banding mosaic viruses (TVBMV). LAMP primers are designed according to a case protein gene conserved region of the tobacco viruses, and a fast, sensitive and high-specificity method is built for detecting various main tobacco viruses by the one-step RT-LAMP technology. A newmeasure is provided for the fast detection of the tobacco viruses.

The invention discloses a tobacco PDS gene fragment-containing cucumber mosaic virus RNA2 attenuated mutant type plasmid vector and application thereof. The plasmid vector contains a CMVFny strain RNA2 complete sequence, and a tobacco PDS gene 199bp fragment is inserted behind a 2a protein termination codon. The plasmid vector and a wild CMVFny RNA1 and wild CMVFny RNA3-containing plasmid are inoculated to nicotiana benthamiana through an agrobacterium infiltration method, so that weakly pathogenic symptoms can be displayed, and the capacity of inducing gene silencing can be judged with nakedeyes without depending on an instrument; great convenience is provided for deep researches on attenuated vaccines.

The invention discloses a synthetic method and bioactivity of beta-amino acid esters which have optical activity and contain benzothiazole groups. The beta-amino acid esters which have optical activity and contain the benzothiazole groups have the structure shown in the general formula (I), and in the formula, R1 is a group of hydrogen, p-chloro, o-chloro, p-fluoro, o-fluoro, p-methyl, o-methoxy and the like; R2 is a group of 4-methyl, 6-methoxy and the like; and R3 is a group of methyl, ethyl, propyl, isopropyl and the like. The invention introduces Mannich reaction which uses organic catalyst of cinchona alkaloid thiourea to catalyze benzothiazole imine and malonate, the product is obtained by one-step synthesis, and the reaction yield and the selectivity to enantiomer are both high. Compounds c, d and e in the inveniton have high therapeutic and passivation inhibiting effects towards tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) and show good anti-plant virus activity.

The invention belongs to the field of biotechnology, and provides an RNAi vector pGEM-PCTRi capable of mediating the resistance to tobacco viral diseases in China. Analyzing the genome characteristics of main virus groups in China, which comprise Potato Virus Y (PVY), Cucumber Mosaic Virus (CMV) and Tobacco Mosaic Virus (TMV) and then selecting three segments of conserved effective sequences capable of inducing RNAi in PVY CP, CMV RNA1 and TMV CP; and integrating the three segments in a tabling manner and constructing an inverted repeat sequence so as to obtain an RNAi vector pGEM-PCTRi. Massproduction of dsRNA can be realized in escherichia coli by utilizing the vector, and the resistance of tobacco to PVY, CMV and TMV can be induced to generate after the tobacco treatment. The invention has the potential of being applicable to the prevention of viral diseases for tobacco farmland production, and endowed with the advantages of broad spectrum, high efficiency, no reference to transgenosis, and the like.

The invention provides a PCR primer combination for Passiflora edulia Sims cucumber mosaic virus and a detecting method. Particularly, the PCR primer combination comprises 3 Passiflora edulia Sims cucumber mosaic virus coat protein specific primers and 2 Passiflora edulia Sims general reference gene EF1a specific primers. Through the design of the 3 Passiflora edulia Sims cucumber mosaic virus coat protein specific primers, 3 specific sites of the virus can be detected at the same time, and occurrence of false positive is avoided; by using the two Passiflora edulia Sims EF1a specific primers,the Passiflora edulia Sims cDNA synthesis quality can be detected, and occurrence of false negative is avoided. The whole detection process can be completed within 4 hours, PCR result detection can beconducted by using 1% agarose, an expensive fluorescent quantatitive PCR instrument is not needed, and the PCR primer combination has the advantages of being fast, economical, high in sensitivity andhigh in stability.

The invention discloses an antiviral agent containing coriolus versicolor polysaccharide, comprising 5-7 wt% of coriolus versicolor polysaccharide, 2-3wt% of surfactant, 1-3 wt% of antifreeze agent, 0.1-0.5 wt% of preservative, 1-3 wt% of stabilizing agent, and the balance of water, wherein the summation of the weight percentages of the raw materials is 100 wt%. The antiviral agent containing coriolus versicolor polysaccharide is accord with the quality requirements of pesticide preparations, has good control effect on Cucumber mosaic virus, CMV, has growth promoting effect on crops, can improve the disease resistance of the crops. In addition, the antiviral agent disclosed herein has high safety for human, animal and environment, thus the antiviral agent is an ideal biopesticide for developing efficient, green and ecological agriculture.

The invention discloses a quinazoline-containing 1,4-pentadiene-3-ketone oxime ether derivative. The quinazoline-containing 1,4-pentadiene-3-ketone oxime ether derivative is characterized in that thegeneral formula is shown in the description, wherein R1 is phenyl, substituted phenyl or substituted heteroaromatic ring radical; R2 is phenyl, substituted phenyl or substituted heteroaromatic ring radical; R3 is one or more of hydrogen atoms, methoxyl groups, nitryl groups, methyl groups, trifluoromethyl groups or halogen atoms at 5-, 6-, 7- or 8-position of quinazoline. The compound provided bythe invention has good treatment and protection effects on cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV), has higher plant virus activity resistance, and can be applied to preparation ofpesticides for resisting plant viruses.

The invention discloses a preparation method and biological activity of a pentadiene ketone compound, containing 1,3,4-oxadiazole sulfo-ethyoxyl, with a function of preventing and treating a plant virus disease, namely a compound expressed in a general formula (I) and a preparation method of the compound. According to the method, by using substituted carboxylic acid, hydrazine hydrate, carbon disulfide, acetone, 4-substituted hydroxy benzaldehyde, substituted aromatic aldehyde, 1,2-dibromoethane and the like as raw materials, methanol, ethanol and N,N-dimethylformamide as solvents and potassium carbonate, sodium hydroxide and potassium hydroxide as catalysts, pentadiene ketone compound containing 1,3,4-oxadiazole sulfo-ethyoxyl is prepared through seven-step synthesis. The compounds I3, I4, I6, I11, I13, I14, I16 and I17 have good functions in cucumber mosaic virus, tobacco mosaic virus, southern rice black-streaked dwarf virus and the like. The formula is shown in the specification.

The invention relates to a method for cultivating tobacco capable of resisting various viruses by adopting an RNAi (RNA interference) technique. The method comprises the following steps of: obtaining relatively conservative areas of four kinds of viruses within a genome range through screening by comparing full-length sequences of multiple genomes of four kinds of viruses, i.e. CMV (cucumber mosaic virus), PVY (potato virus Y), PVX (potato virus X) and TMV (tobacco mosaic virus) in a genBank; selecting virus sequences and artificially synthesizing 800bp mosaic genes; and accordingly constructing RNAi plant expression carriers of the mosaic genes and transforming common tobacco through agrobacterium to obtain transgenic plants. The method for cultivating tobacco capable of resisting various viruses by adopting the RNAi technique has the characteristics that the four kinds of major tobacco viruses in China are selected as targets, the artificially constructed hairpin structures comprising the sequences of the four kinds of viruses are transformed into the tobacco by using the plant genetic engineering technique, a hairpin double-strand RNA structure transcribed by the tobacco is cut into siRNA (small interfering RNA) by the plant self mechanism, the normal duplication and the accumulation of target virus genes in tobacco plants are specifically interfered, degraded or silenced, and new tobacco materials capable of resisting various viruses can be obtained through screening.

The present invention relates to virus-like particles of plant virus Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising Th cell epitopes, in particular universal Th cell epitopes. Furthermore, these modified VLPs serve as, preferably, vaccine platform, for generating immune responses, in particular antibody responses, against antigens linked to said modified VLPs. The presence of the Th cell epitopes, in particular universal Th cell epitopes, led to a further increase in the generated immune response.

The invention discloses a duplex attenuated vaccine capable of resisting cucumber mosaic virus and potato virus X and application of the duplex attenuated vaccine. The duplex attenuated vaccine is a cucumber mosaic virus RNA2 attenuated mutant plasmid vector pCCFR2-2bPTII-PX1967-2166 inserted with a PVX gene segment; the nucleotide sequence of the PVX gene segment is shown as SEQ ID NO. 1, and thenucleotide sequence of the duplex attenuated vaccine is shown as SEQ ID NO. 2. The duplex attenuated vaccine has a cross protection effect on CMV and PVX; and a vaccine material and an effective prevention and treatment measure are provided for preventing and treating plant virus diseases caused by CMV and PVX in the field.

The invention discloses epsilon-polylysine-containing pesticidal composition for controlling plant virus diseases. The epsilon-polylysine-containing pesticidal composition comprises components as follows: epsilon-polylysine, glycine, cytosintetidemycin and filler. The pesticidal composition has the advantages as follows: 1, after epsilon-polylysine, glycine and cytosintetidemycin are mixed in a certain ratio, the antiviral synergism is remarkable; 2, the disease prevention spectrum is broad, and the pesticidal composition has obvious control effect on multiple virus diseases such as rice stripe virus, cucumber mosaic virus, tobacco mosaic virus and the like of multiple host plants; 3, the plants are induced to produce immunity, defense capability of the plants can be utilized sufficiently,use of chemical pesticides and risk of pesticide resistance generation are reduced, ecological environment is protected, and requirements of green plant protection and ecological civilization construction are met.

The invention discloses a quadruple RT-PCR method simultaneously detecting multiple kinds of pepper viruses and an application of the method.The method comprises the steps that firstly, tender pepper blades with mosaic symptoms in a filed are selected, saved on ice and brought back to a laboratory, and RNA is extracted through a Trizol method; secondly, the RNA obtained in the first step serves as a template, 6-base Random Primers serve as reverse primers, and by means of inverse transcription, cDNA is obtained; thirdly, the cDNA obtained in the second step serves as a template, and PCR amplification is conducted on the tender pepper blades through four pairs of primers; fourthly, PCR products are subjected to gel electrophoresis, a gel-imaging system takes pictures, and the virus infection situation is judged according to the size of a target fragment and positive control.According to the detection method, one RT-PCR is used for simultaneously detecting main pepper viruses including a cucumber mosaic virus (CMV), a tobacco mosaic virus (TMV), a potato virus Y (PVY) and a broad bean wild virus-2 (BBWV-2).The kinds of detected virus are increased, the detection efficiency is improved, and the detection cost is low.

The invention relates to a seedling growth matrix capable of reducing plant virus harm and a preparation method and application thereof. The seedling growth matrix is composed of mushroom residue fermentation rotten matters, grass carbon and vermiculite. The disease index of the seedling growth matrix is more than 40 percent lower than that of a grass carbon matrix on average. The average prevention efficiency of the seedling growth matrix on cucumber mosaic viruses, tomato yellow leaf curl viruses and tobacco mosaic viruses is respectively 40 percent, 40 percent and 50 percent. The seedling growth matrix is simple in production process, low in cost and capable of reducing plant virus harm and usage amount of the grass carbon, and has remarkable economic benefit and economic benefit.

The invention discloses a surface plasma resonance detecting method for PVY/CMV (Potato Virus Y/Cucumber Mosaic Virus) and belongs to a material detection method. The surface plasma resonance detecting method comprises the following steps: 1) preparing a PVY/CMV antigen and a monoclonal body; 2) preparing a biosensor chip; 3) preparing a standard curve and acquiring a virus computational formula; and then measuring the content of PVY/CMV by utilizing the virus computational formula. The surface plasma resonance detecting method provided by the invention has high sensitivity, can be used for qualitatively and quantitatively measuring PVY/CMV and is convenient in operation and low in cost.

The invention discloses a plant source antiviral agent which takes a phyllanthi fructus L. extractive as an effective component and a preparation method thereof. The preparation method comprises the following steps: crushing phyllanthi fructus L. and carrying out a certain processing process to prepare a phyllanthi fructus L. antiviral soluble liquid agent, an aqueous agent and micro-emulsion. The antiviral agent can be used for preventing and treating tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), potato virus X (PVX), potato virus Y (PVY) and the like on crops including tobaccos, chilies, tomatoes, pumpkins, potatoes and the like. The preparation contains 10%-30% of the phyllanthi fructus L. extractive and a residual amount of an auxiliary agent. The plant antiviral agent not only has a good antiviral effect, but also is safe to environments, human beings, livestock and natural enemies of pests, and other beneficial organisms. The plant antiviral agent is simple in preparation process and low in cost, and is suitable for popularization and application.

The invention relates to a pesticide composition which comprises the active ingredients such as aureonucleomycin and xinjunan, wherein the mass ratio of the aureonucleomycin to the xinjunan is (1-80):(80-1), and the content of the aureonucleomycin and xinjunan in the compound preparation is 1-80%. The pesticide composition can be prepared into such dosage forms as suspending agents, wettable powder, water dispersible granules, emulsion in water, microemulsion and the like by the method known by technicians in the field. The pesticide composition can be used for controlling various virus diseases of crops, such as tobacco mosaic virus diseases, cucumber mosaic virus diseases, maize dwarf mosaic virus diseases, rice stripe diseases, rice dwarf diseases and the like.

The invention discloses a TMV-CMV-PVY three-virus (tobacco mosaic virus, cucumber mosaic virus and potato virus) colloidal gold rapid test strip, which is composed of a sample pad, a colloidal gold pad, an NC membrane, a piece of water-absorbent filter paper and a backing, wherein a colloidal gold labeled TMV specific antibody, a CMV specific antibody and a PVY specific antibody are enveloped on the colloidal gold pad, and the three antibodies are secreted from hybridoma cell strains BALB/c 15 50, BALBc 15 27 and BALBc 15 8; a detection line and a control line are arranged on the NC membrane; specific antigens of three viruses are enveloped on the detection line; and a colloidal gold labeled second antibody is enveloped on the control line. The test strip is especially suitable for detecting the TMV, CMV and PVY in Guangdong tobacco-growing areas, and the test strip is rapid, sensitive, accurate, low in cost and convenient to operate; the test strip is capable of simultaneously diagnosing various viruses by conducting sampling once; the test strip is quite suitable for in-situ preliminary screening of a great batch of samples; and the test strip, in actual production, has a high application value, and the test strip has a good popularization and application prospect.

The invention relates to a pesticide composition whose effective components are Cytosinpeptidemycin and Xinjunan. A weight ratio of the Cytosinpeptidemycin and the Xinjunan is 1-80:80-1, and content of the Cytosinpeptidemycin and the Xinjunan in a compound preparation is 1-80%. By employing methods widely known by those skilled in the art, the pesticide composition can be prepared in various formulations such as suspending agent, wettability pulvis, water dispersion granule, emulsion in water, micron emulsion and the like. The pesticide composition in the invention can be used for controlling a plurality of crop viroses such as tobacco mosaic virus disease, cucumber mosaic virus disease, maize dwarf mosaic virus disease, rice stripe disease, rice dwarf disease and the like.

The invention provides a cucumber mosaic virus induced gene silencing (CMV-ZMBJ VIGS) system. The RNAs (ribonucleic acids) of an virion of the isolate CMV-ZMBJ which is separated from CMV naturally infecting corn in the corn field are used as raw materials, after the full-length cDNAs of RNA1, RNA2 and RNA3 in a genome are amplified by RT-PCR (reverse transcription-polymerase chain reaction), the full-length cDNAs are linked to binary expression vectors of a plant, such as pCass-Rz, and then is transformed in agrobacterium to achieve infectivity, and thus CMV-ZMBJ infected cDNA cloned pCMV101, pCMV201 and pCMV301 are constructed successfully. The constructed CMV-ZMBJ VIGS system is suitable for researching the functions of a variety of plant genes and can be used for silencing the genes easily, quickly and efficiently at a low cost only by inserting a part of segments of plant genes to be researched into a vector without carrying out genetic transformation. In addition, a CMV-ZMBJ VIGS vector can be used for researching the functions of corn genes, and compared with the existing BMV (brome mosaic virus) VIGS vector which is usually used for researching the functions of corn genes; the CMV-ZMBJ VIGS vector is applicable to more hosts, including corn varieties such as Va35, B73, Zheng 58 and Mo17.

The invention provides an InDel marker of a cucumber anti-cucumber-mosaic-virus-disease gene cmv and its application, belonging to the field of biotechnological assistant breeding. The nucleotide sequence of a primer InDel-cmv3-F/ InDel-cmv3-R for the InDel marker closely linked with the cucumber anti-cucumber-mosaic-virus-disease gene cmv is as described in the specification; a characteristic band amplified by the InDel marker and closely linked with the anti-cucumber-mosaic-virus-disease gene cmv has a length of 223 bp; and a characteristic band amplified by the InDel marker and closely linked with a cucumber-mosaic-virus-disease susceptibility gene CMV has a length of 245bp. The marker provided by the invention has the advantages of high efficiency and little limitation, improves the efficiency of breeding of anti-cucumber-mosaic-virus-disease cucumber materials and shortens a breeding cycle.

The invention discloses a preparation method of test strip for rapid field detection of tobacco cucumber mosaic virus, which comprises the following steps of: preparing an antibody, preparing colloidal gold, preparing a colloidal gold-antibody conjugate, preparing a nitrocellulose filter, and preparing a colloidal gold chromatographic test strip. The prepared test strip for rapid field detection of tobacco cucumber mosaic virus according to the technical proposal has an optimum detection temperature of 15-35DEG C, sample detection amount of 0.1-0.3g and tobacco sample area of 3-5cm<2>. The detection method comprises charging tobacco leaf in a plastic bag containing buffer, grinding, immersing the test strip in the buffer, reacting for 30min, and observing the result. The false positive rate is less than 1%. The invention has the advantages of convenient application and high reliability; and is especially suitable for rapid field detection.