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3 results about "Cucumber mosaic virus" patented technology
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Cucumber mosaic virus (CMV) is a plant pathogenic virus in the family Bromoviridae. It is the type member of the plant virus genus, Cucumovirus. This virus has a worldwide distribution and a very wide host range. In fact it has the reputation of having the widest host range of any known plant virus. It can be transmitted from plant to plant both mechanically by sap and by aphids in a stylet-borne fashion. It can also be transmitted in seeds and by the parasitic weeds, Cuscuta sp. (dodder).
A plasmid containing modified satellite RNA, construction method and application thereof
The application provides a plasmid containing modified satellite RNA and a construction method and application, and belongs to the field of plant virology and molecular biology. Each copy number of the modified satellite RNA is composed of a modified satRNA TA-Tb fragment and an HDV ribozyme fragment, and the copy number of the modified satellite RNA in the plasmid is 10. The nucleotide sequence of the modified satellite RNA is shown in SEQ No. 3. After the plasmid of the application is inoculated by agrobacterium injection, the symptoms caused by cucumber mosaic virus on different strains and the same strain (CMV Fny ) on different hosts can be significantly reduced. An effective control measure for field control of cucumber mosaic virus is provided.
Owner:QINGDAO AGRI UNIV
Fusion-modified virus-like particles of cmv
The present invention relates to a modified virus-like particle (VLP) of Cucumber Mosaic Virus (CMV) comprising at least one fusion protein, wherein the at least one fusion protein comprises or preferably consists of b) a chimeric CMV polypeptide, wherein the chimeric CMV polypeptide comprises or preferably consists of (iii) a CMV polypeptide, wherein the CMV polypeptide comprises a coat protein of CMV; and (iv) an antigen polypeptide, wherein the antigen polypeptide is inserted into the CMV polypeptide, wherein the insertion of the antigen polypeptide is between amino acid residues corresponding to position 84 and position 85 of SEQ ID NO: 62 of the CMV polypeptide; and (iii) a T helper cell epitope, wherein the T helper cell epitope replaces an N-terminal region of the CMV polypeptide, and wherein preferably the N-terminal region of the CMV polypeptide corresponds to amino acids 2-12 of SEQ ID NO: 62.
Owner:SAIBA AG
Preparation method of artificial defective interfering RNA and application thereof in anti-cucumber mosaic virus
PendingCN122104711AVector-based foreign material introductionAngiosperms/flowering plantsOpen reading frameCucumber mosaic virus
The application belongs to the technical field of biology, and particularly relates to a preparation method of artificial defective interfering RNA and application thereof in resisting cucumber mosaic virus. The application discloses an artificial defective interfering RNA, which comprises the following elements in the direction from the 5' end to the 3' end: a 5' end UTR of a CMV genomic RNA3, an IGR, a CP open reading frame and a 3' end UTR sequence. The application also simultaneously provides a recombinant DNA for coding the artificial defective interfering RNA. The application also simultaneously provides a recombinant expression vector containing the above-mentioned recombinant DNA. The application also simultaneously provides application of the above-mentioned artificial defective interfering RNA, the recombinant DNA and the recombinant expression vector in resisting cucumber mosaic virus.
Owner:ZHEJIANG SCI-TECH UNIV